{"id":71088,"date":"2026-11-15T00:09:10","date_gmt":"2026-11-14T16:09:10","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=71088"},"modified":"2026-11-15T00:09:10","modified_gmt":"2026-11-14T16:09:10","slug":"dorado-fast-hac-pod5-read-length-qscore-na24385","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/dorado-fast-hac-pod5-read-length-qscore-na24385\/","title":{"rendered":"Dorado \u5b9e\u8dd1\uff5c\u540c\u4e00\u6761\u7eb3\u7c73\u5b54\u539f\u59cb\u4fe1\u53f7\uff0c\u4e3a\u4ec0\u4e48\u4f1a\u5f97\u5230 4856 \u548c 5058 \u4e24\u79cd\u8bfb\u957f\uff1f"},"content":{"rendered":"
\n

\u5bf9\u540c\u4e00\u4efd POD5 \u91cd\u65b0\u8bc6\u522b\u78b1\u57fa\u540e\uff0c\u8bfb\u6bb5\u540d\u8fd8\u5728\uff0c\u5e8f\u5217\u5374\u53ef\u80fd\u53d8\u957f\uff0c\u5e73\u5747\u8d28\u91cf\u4e5f\u4f1a\u53d8\u5316\u3002\u624b\u5de5\u628a FASTQ \u8d28\u91cf\u503c\u76f8\u52a0\u518d\u53d6\u5e73\u5747\uff0c\u7ecf\u5e38\u590d\u73b0\u4e0d\u51fa\u8f6f\u4ef6\u62a5\u544a\u7684\u6570\u5b57\u3002\u6211\u4eec\u53d6 Oxford Nanopore \u516c\u5f00\u7684\u4e00\u6761\u771f\u5b9e\u539f\u59cb\u4fe1\u53f7\uff0c\u5728\u672c\u5730\u7528 Dorado \u5b8c\u6574\u8dd1\u4e86\u4e24\u6b21\u78b1\u57fa\u8bc6\u522b\uff0c\u5206\u522b\u4f7f\u7528\u5feb\u901f\u6a21\u578b\u4e0e\u9ad8\u51c6\u786e\u6a21\u578b\u3002\u968f\u540e\u9010\u78b1\u57fa\u91cd\u7b97\u6574\u6761\u8bfb\u6bb5\u7684\u8d28\u91cf\u5206\u6570\uff0c\u5e76\u5bf9\u7167\u540c\u4e00\u53c2\u8003\u6838\u7b97\u9519\u914d\u3001\u63d2\u5165\u548c\u5220\u9664\u3002\u4e0b\u9762\u7684\u6570\u5b57\u90fd\u53ef\u4ee5\u4ece\u4e24\u4efd\u8f93\u51fa BAM \u4e2d\u8bfb\u56de\u3002\u5b98\u65b9\u5355\u8bfb\u6bb5\u6d4b\u8bd5\u6570\u636e\u8bf4\u660e<\/a><\/p>\n

\u8ba9\u6bd4\u8f83\u843d\u5728\u540c\u4e00\u6761\u539f\u59cb\u5206\u5b50\u4e0a<\/h2>\n

\u8fd9\u6761\u4fe1\u53f7\u6765\u81ea\u516c\u5f00\u6837\u672c NA24385 \u7684\u4e00\u53f7\u67d3\u8272\u4f53\u533a\u57df\uff0c\u91c7\u7528\u6bcf\u79d2\u4e94\u5343\u4e2a\u91c7\u6837\u70b9\u7684\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6761\u4ef6\u3002\u5b98\u65b9\u8fd8\u7ed9\u51fa\u4e00\u6bb5\u4eba\u4e3a\u52a0\u5165\u4e86\u7b80\u5e76\u78b1\u57fa\u7b26\u53f7\u7684\u53c2\u8003\u5e8f\u5217\uff0c\u7528\u4e8e\u81ea\u5df1\u7684\u683c\u5f0f\u6d4b\u8bd5\u3002\u6211\u4eec\u4f7f\u7528\u540c\u4e00\u4e2a POD5\u3001\u540c\u4e00\u4efd\u53c2\u8003\u3001\u76f8\u540c\u8f6f\u4ef6\u7248\u672c\u4e0e\u8bbe\u5907\u8bbe\u7f6e\uff0c\u663e\u5f0f\u6307\u5b9a\u76f8\u540c\u7248\u672c\u7cfb\u5217\u4e0b\u7684\u4e24\u4e2a\u6a21\u578b\u3002\u5173\u95ed\u53ef\u9009\u5e8f\u5217\u4fee\u526a\uff0c\u4e24\u8f6e\u90fd\u76f4\u63a5\u8f93\u51fa\u5e26\u6bd4\u5bf9\u7684 BAM\u3002\u9996\u5148\u68c0\u67e5\u4e24\u6b21\u7684\u8bfb\u6bb5\u6807\u8bc6\u5b8c\u5168\u4e00\u81f4\uff0c\u624d\u80fd\u8ba4\u5b9a\u6bd4\u8f83\u7684\u662f\u540c\u4e00\u4efd\u539f\u59cb\u4fe1\u53f7\u3002\u518d\u770b\u8bfb\u7ec4\u4e2d\u7684\u6a21\u578b\u540d\u79f0\uff0c\u9632\u6b62\u81ea\u52a8\u9009\u62e9\u7248\u672c\u6216\u8005\u4e2d\u95f4\u6587\u4ef6\u4e32\u7528\u8ba9\u5b9e\u9a8c\u5931\u53bb\u53ef\u6bd4\u6027\u3002Dorado \u6a21\u578b\u548c\u78b1\u57fa\u8bc6\u522b\u8bf4\u660e<\/a><\/p>\n

\u5feb\u901f\u6a21\u578b\u7ed9\u51fa\u56db\u5343\u516b\u767e\u4e94\u5341\u516d\u4e2a\u78b1\u57fa\uff0c\u9ad8\u51c6\u786e\u6a21\u578b\u7ed9\u51fa\u4e94\u5343\u96f6\u4e94\u5341\u516b\u4e2a\uff0c\u76f8\u5dee\u4e8c\u767e\u96f6\u4e8c\u3002\u7535\u6d41\u91c7\u6837\u70b9\u548c\u6a21\u578b\u8f93\u51fa\u7684\u78b1\u57fa\u5c5e\u4e8e\u4e0d\u540c\u7684\u6d4b\u91cf\u5355\u4f4d\uff0c\u4e00\u6bb5\u4fe1\u53f7\u7531\u591a\u4e2a\u91c7\u6837\u70b9\u7ec4\u6210\uff0c\u6a21\u578b\u9700\u8981\u63a8\u65ad\u5176\u4e2d\u7684\u78b1\u57fa\u987a\u5e8f\u548c\u6570\u91cf\u3002\u8fd9\u91cc\u80fd\u76f4\u63a5\u786e\u8ba4\u7684\u662f\u540c\u4e00\u8bfb\u540d\u5f97\u5230\u4e0d\u540c\u7684\u78b1\u57fa\u6570\uff0c\u4e0d\u80fd\u5149\u9760\u957f\u5ea6\u5dee\u5224\u5b9a\u5feb\u901f\u6a21\u578b\u6070\u597d\u6f0f\u6389\u4e86\u4e8c\u767e\u96f6\u4e8c\u4e2a\u6307\u5b9a\u4f4d\u7f6e\u3002\u8981\u8fdb\u4e00\u6b65\u5b9a\u4f4d\u5dee\u5f02\uff0c\u8fd8\u5f97\u770b\u4e24\u6b21\u8f93\u51fa\u5404\u5bf9\u9f50\u5230\u54ea\u4e00\u6bb5\u53c2\u8003\uff0c\u526a\u5207\u6389\u4e86\u591a\u5c11\uff0c\u4e24\u8fb9\u5b8c\u5168\u53ef\u6bd4\u8f83\u7684\u533a\u95f4\u53c8\u6709\u591a\u957f\u3002<\/p>\n

\u4e3a\u4ec0\u4e48\u76f4\u63a5\u5e73\u5747\u8d28\u91cf\u503c\u4f1a\u9ad8\u51fa\u5341\u51e0\u5206<\/h2>\n

\u4e00\u4e2a\u78b1\u57fa\u7684\u8d28\u91cf\u5206\u6570\u662f\u9519\u8bef\u6982\u7387\u7684\u8d1f\u5bf9\u6570\u53d8\u6362\u3002\u53d6\u8bfb\u6bb5\u5e73\u5747\u8d28\u91cf\u65f6\uff0c\u5e94\u8be5\u5148\u5c06\u6bcf\u4e2a\u4f4d\u7f6e\u6362\u56de\u9519\u8bef\u6982\u7387\uff0c\u628a\u8fd9\u4e9b\u6982\u7387\u6c42\u7b97\u672f\u5e73\u5747\uff0c\u518d\u8f6c\u6210\u4e00\u4e2a\u8d28\u91cf\u5206\u6570\u3002\u62ff\u4e00\u534a\u78b1\u57fa\u8d28\u91cf\u4e3a\u5341\u3001\u4e00\u534a\u4e3a\u4e09\u5341\u7684\u4f8b\u5b50\uff0c\u9519\u8bef\u6982\u7387\u5206\u522b\u4e3a\u5341\u5206\u4e4b\u4e00\u548c\u5343\u5206\u4e4b\u4e00\uff0c\u5e73\u5747\u4e3a\u96f6\u70b9\u96f6\u4e94\u96f6\u4e94\u3002\u518d\u8f6c\u56de\u8d28\u91cf\u5927\u7ea6\u5341\u4e09\u3002\u76f4\u63a5\u5e73\u5747\u4e24\u4e2a\u8d28\u91cf\u5206\u6570\u4f1a\u5f97\u5230\u4e8c\u5341\uff0c\u663e\u8457\u4f4e\u4f30\u8fd9\u6279\u78b1\u57fa\u7684\u5e73\u5747\u9519\u8bef\u6982\u7387\u3002\u8fd9\u4e5f\u662f\u4e3a\u4ec0\u4e48\u4e00\u5c0f\u6bb5\u5dee\u8d28\u91cf\u5e8f\u5217\u80fd\u591f\u62c9\u4f4e\u6574\u6761\u8bfb\u6bb5\u7684\u5e73\u5747\u8d28\u91cf\uff0c\u5c3d\u7ba1\u4f59\u4e0b\u7684\u5927\u90e8\u5206\u4f4d\u7f6e\u8d28\u91cf\u90fd\u5f88\u9ad8\u3002<\/p>\n

\u8fd8\u8981\u590d\u73b0\u5b98\u65b9\u5bf9\u5e8f\u5217\u5f00\u5934\u7684\u5904\u7406\u3002\u5bf9\u4e8e\u957f\u5ea6\u8d85\u8fc7\u516d\u5341\u7684\u8bfb\u6bb5\uff0cDorado \u7684\u6574\u6761\u8d28\u91cf\u7edf\u8ba1\u5148\u7565\u53bb\u6d4b\u5e8f\u65b9\u5411\u4e0a\u7684\u524d\u516d\u5341\u4e2a\u78b1\u57fa\u3002\u53ef\u4ee5\u4ece BAM \u53d6\u56de\u539f\u59cb\u65b9\u5411\u7684\u9010\u78b1\u57fa\u8d28\u91cf\uff0c\u53ea\u4e3a\u8fd9\u4e2a\u7edf\u8ba1\u91cf\u622a\u53bb\u5f00\u5934\uff0c\u518d\u6362\u7b97\u6982\u7387\u3002\u8fd9\u4e2a\u6b65\u9aa4\u4e0d\u4f1a\u628a\u8f93\u51fa FASTA \u7684\u957f\u5ea6\u518d\u51cf\u516d\u5341\u3002\u5b9e\u8dd1\u4e2d\uff0c\u5feb\u901f\u6a21\u578b\u7684\u9010\u4f4d\u8d28\u91cf\u76f4\u63a5\u5e73\u5747\u4e3a\u4e8c\u5341\u4e5d\u70b9\u516b\u516d\uff0c\u8f6c\u6362\u4e3a\u6982\u7387\u5e76\u5728\u5168\u957f\u6c42\u5e73\u5747\u5f97\u5230\u5341\u516d\u70b9\u96f6\u56db\uff0c\u6309\u5b98\u65b9\u89c4\u5219\u7565\u53bb\u5f00\u5934\u540e\u5f97\u5230\u5341\u516d\u70b9\u4e8c\u4e03\uff0c\u4e0e BAM \u6807\u7b7e\u4e00\u81f4\u3002\u9ad8\u51c6\u786e\u6a21\u578b\u7684\u4e09\u4e2a\u6570\u5b57\u5206\u522b\u4e3a\u56db\u5341\u4e00\u70b9\u4e00\u56db\u3001\u4e8c\u5341\u4e00\u70b9\u4e03\u4e8c\u548c\u4e8c\u5341\u4e8c\u70b9\u56db\u4e8c\u3002Dorado \u5bf9\u5e73\u5747\u8d28\u91cf\u7684\u5b9a\u4e49<\/a><\/p>\n

\n\n\n\n\n\n\n\n\n\n
\u540c\u4e00\u6761\u771f\u5b9e\u4fe1\u53f7<\/th>\nfast 5.2.0<\/th>\nhac 5.2.0<\/th>\n<\/tr>\n<\/thead>\n
\u8f93\u51fa\u78b1\u57fa\u6570<\/td>\n4856<\/td>\n5058<\/td>\n<\/tr>\n
BAM \u6574\u6761\u8d28\u91cf qs<\/code><\/td>\n16.2727<\/td>\n22.4214<\/td>\n<\/tr>\n
\u76f4\u63a5\u5e73\u5747\u5168\u90e8\u9010\u78b1\u57fa\u8d28\u91cf<\/td>\n29.8589<\/td>\n41.1394<\/td>\n<\/tr>\n
\u6bd4\u5bf9\u533a\u5185\u66ff\u6362\u3001\u63d2\u5165\u78b1\u57fa\u3001\u5220\u9664\u78b1\u57fa<\/td>\n66\u300119\u300130<\/td>\n7\u30010\u30010<\/td>\n<\/tr>\n
\u6bd4\u5bf9\u8d28\u91cf MAPQ<\/code><\/td>\n60<\/td>\n60<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u540c\u4e00\u6761\u4fe1\u53f7\uff0c\u4e24\u79cd\u8bfb\u957f\u3002\u5728\u4e00\u6761\u771f\u5b9e
\u5728\u4e00\u6761\u771f\u5b9e NA24385 \u8bfb\u6bb5\u4e0a\u8fd0\u884c\u4e24\u79cd\u6a21\u578b\uff0c\u91cd\u65b0\u8ba1\u7b97\u6574\u6761\u8bfb\u6bb5\u7684\u8d28\u91cf\u3002<\/figcaption><\/figure>\n

\u5bfc\u51fa\u8d28\u91cf\u65f6\u8fd8\u4f1a\u9047\u5230\u65b9\u5411\u95ee\u9898\u3002\u4ee5\u53cd\u5411\u94fe\u6bd4\u5230\u53c2\u8003\u7684\u8bfb\u6bb5\uff0c\u5728\u6bd4\u5bf9\u6587\u4ef6\u91cc\u7ed9\u51fa\u7684\u5e8f\u5217\u548c\u8d28\u91cf\u53ef\u80fd\u5df2\u7ecf\u968f\u6bd4\u5bf9\u65b9\u5411\u53cd\u8f6c\u3002\u7565\u53bb\u524d\u516d\u5341\u4e2a\u4f4d\u7f6e\u65f6\u5e94\u4ece\u6d4b\u5e8f\u539f\u65b9\u5411\u5f00\u59cb\u3002\u5728\u5b9e\u73b0\u65f6\u76f4\u63a5\u53d6\u6062\u590d\u539f\u65b9\u5411\u7684\u8d28\u91cf\u6570\u7ec4\uff0c\u518d\u53e6\u884c\u8bfb\u53d6\u53c2\u8003\u65b9\u5411\u7684\u6bd4\u5bf9\u5217\uff0c\u4fbf\u53ef\u4f7f\u4e24\u4e2a\u7edf\u8ba1\u5bf9\u5e94\u5404\u81ea\u7684\u5750\u6807\u7ea6\u5b9a\u3002<\/p>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u628a\u8d28\u91cf\u4f30\u8ba1\u4e0e\u53c2\u8003\u6bd4\u5bf9\u5206\u5f00\u590d\u6838<\/h2>\n
dorado basecaller models\/dna_r10.4.1_e8.2_400bps_fast@v5.2.0 chr1_MAT.pod5 --device cpu --no-trim --reference chr1_MAT_iupac.fasta > fast.bam\r\ndorado basecaller models\/dna_r10.4.1_e8.2_400bps_hac@v5.2.0 chr1_MAT.pod5 --device cpu --no-trim --reference chr1_MAT_iupac.fasta > hac.bam\r\nsamtools view fast.bam | cut -f1-6\r\nsamtools view hac.bam | cut -f1-6\r\n<\/code><\/pre>\n
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