{"id":71236,"date":"2026-11-15T00:18:22","date_gmt":"2026-11-14T16:18:22","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=71236"},"modified":"2026-11-15T00:18:22","modified_gmt":"2026-11-14T16:18:22","slug":"masurca-superreads-kmer-overlap-distinct-reference-bases","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/masurca-superreads-kmer-overlap-distinct-reference-bases\/","title":{"rendered":"MaSuRCA \u5b9e\u8dd1\uff5c8800 \u6761\u77ed\u8bfb\u6bb5\u53d8\u6210\u4e24\u6761 super-read\uff0c\u80fd\u5f53\u6210\u4e24\u6761\u72ec\u7acb\u957f\u5206\u5b50\u5417\uff1f"},"content":{"rendered":"
\n

\u505a\u6df7\u5408\u7ec4\u88c5\u65f6\uff0cMaSuRCA \u53ef\u4ee5\u628a\u5927\u91cf\u77ed\u8bfb\u6bb5\u7684\u91cd\u53e0\u4fe1\u606f\u6574\u7406\u4e3a\u5c11\u6570\u8f83\u957f\u7684 super-read\u3002\u8fd9\u91cc\u7684\u957f\u5e8f\u5217\u6709\u5b9e\u9645\u78b1\u57fa\u5185\u5bb9\uff0c\u53ef\u4ee5\u4f5c\u4e3a\u7ec4\u88c5\u8bc1\u636e\uff0c\u4e5f\u5f88\u5bb9\u6613\u88ab\u8bef\u5199\u6210\u539f\u59cb\u957f\u8bfb\u6bb5\u3002\u6211\u4eec\u7528\u56db\u5343\u56db\u767e\u5bf9\u6a21\u62df\u77ed\u8bfb\u6bb5\u6784\u5efa super-read\uff0c\u8ffd\u67e5\u6bcf\u6761\u8f83\u957f\u5e8f\u5217\u6765\u81ea\u54ea\u6bb5\u6a21\u677f\uff0c\u53c8\u8ba9\u4e09\u79cd\u56fe\u6784\u5efa\u53c2\u6570\u9762\u5bf9\u540c\u4e00\u4e2a\u4e94\u5341\u516d\u78b1\u57fa\u91cd\u590d\u3002\u5c24\u5176\u503c\u5f97\u590d\u6838\u7684\u662f\uff0c\u4e24\u4e2a\u6210\u529f\u8fd0\u884c\u7684\u53c2\u6570\u5404\u7ed9\u51fa\u4e24\u6761\u957f\u5e8f\u5217\uff0c\u603b\u957f\u5ea6\u5f88\u63a5\u8fd1\uff0c\u4f46\u540c\u4e00\u6bb5\u771f\u5b9e\u5e8f\u5217\u88ab\u8868\u793a\u4e86\u591a\u5c11\u6b21\u5e76\u4e0d\u76f8\u540c\u3002MaSuRCA \u4f5c\u8005\u9879\u76ee\u4e0e\u914d\u7f6e\u8bf4\u660e<\/a><\/p>\n

\u5148\u7ed9\u91cd\u590d\u533a\u4e00\u4e2a\u5df2\u77e5\u7b54\u6848<\/h2>\n

\u6784\u9020\u4e00\u6761\u957f\u4e8c\u4e07\u4e09\u5343\u4e00\u767e\u4e00\u5341\u4e8c\u78b1\u57fa\u7684\u7ebf\u6027\u6a21\u677f\uff0c\u5728\u4e09\u4e2a\u4e0d\u540c\u968f\u673a\u5e8f\u5217\u6bb5\u4e4b\u95f4\u63d2\u5165\u4e24\u4efd\u5b8c\u5168\u76f8\u540c\u7684\u4e94\u5341\u516d\u78b1\u57fa\u5e8f\u5217\u3002\u6bcf\u5bf9\u77ed\u8bfb\u6bb5\u6765\u81ea\u540c\u4e00\u4e2a\u6a21\u677f\u7247\u6bb5\uff0c\u4e24\u7aef\u5404\u957f\u4e00\u767e\u78b1\u57fa\uff0c\u7247\u6bb5\u957f\u5ea6\u56f4\u7ed5\u4e09\u767e\u78b1\u57fa\u53d8\u5316\u3002\u603b\u8f93\u5165\u516b\u5343\u516b\u767e\u4e2a\u8bfb\u6bb5\u7aef\u3001\u516b\u5341\u516b\u4e07\u6d4b\u5e8f\u78b1\u57fa\u3002\u8fd9\u4e2a\u6570\u9664\u4ee5\u6a21\u677f\u957f\u5ea6\u662f\u539f\u59cb\u5e73\u5747\u6d4b\u5e8f\u6df1\u5ea6\u7684\u8fd1\u4f3c\u503c\u3002\u628a\u5b83\u548c\u6700\u7ec8 super-read \u603b\u957f\u5ea6\u6df7\u7528\uff0c\u4f1a\u8bef\u4ee5\u4e3a\u540e\u8005\u6ca1\u80fd\u4fdd\u4f4f\u8f93\u5165\u78b1\u57fa\u3002\u6a21\u62df\u5668\u53cd\u590d\u4ece\u540c\u4e00\u6a21\u677f\u53d6\u6837\u672c\u6765\u5c31\u5236\u9020\u4e86\u8986\u76d6\u5197\u4f59\uff0c\u800c\u6784\u5efa\u957f\u5e8f\u5217\u6b63\u662f\u4e3a\u4e86\u538b\u7f29\u8fd9\u7c7b\u91cd\u590d\u89c2\u5bdf\u3002<\/p>\n

\u5b9e\u9a8c\u53ea\u6267\u884c\u5230\u914d\u7f6e\u5141\u8bb8\u7684 super-read \u751f\u6210\u9636\u6bb5\u3002\u8f93\u5165\u7684\u8bfb\u6bb5\u3001\u6587\u5e93\u7247\u6bb5\u5747\u503c\u4e0e\u6807\u51c6\u5dee\u3001\u7ebf\u7a0b\u6570\u548c\u8ba1\u6570\u914d\u7f6e\u59cb\u7ec8\u76f8\u540c\uff0c\u5206\u522b\u7528\u4e09\u5341\u4e00\u3001\u56db\u5341\u4e00\u548c\u4e03\u5341\u4e00\u78b1\u57fa\u7684\u77ed\u8bcd\u957f\u5ea6\u6784\u56fe\u3002\u8fd9\u91cc\u7684\u77ed\u8bcd\u957f\u5ea6\u63cf\u8ff0\u8bc6\u522b\u5c40\u90e8\u5e8f\u5217\u7684\u7a97\u53e3\uff0c\u65e2\u5f71\u54cd\u54ea\u4e9b\u91cd\u590d\u7a97\u53e3\u4f1a\u6df7\u5728\u4e00\u8d77\uff0c\u4e5f\u5f71\u54cd\u771f\u5b9e\u6d4b\u5e8f\u6709\u9519\u8bef\u65f6\u6709\u6548\u7a97\u53e3\u7684\u6570\u91cf\u3002\u6211\u4eec\u7684\u4eba\u5de5\u8f93\u5165\u6ca1\u6709\u6d4b\u5e8f\u9519\u8bef\uff0c\u5e76\u4e14\u542b\u6709\u4e00\u4e2a\u523b\u610f\u8bbe\u8ba1\u7684\u91cd\u590d\uff0c\u9002\u5408\u89c2\u5bdf\u53c2\u6570\u5e26\u6765\u7684\u5dee\u522b\u3002\u5b9e\u9645\u6742\u5408\u6216\u6709\u9519\u6570\u636e\u4e2d\uff0c\u8d38\u7136\u589e\u52a0\u7a97\u53e3\u957f\u5ea6\u53ef\u80fd\u4f7f\u539f\u672c\u5c31\u6d45\u7684\u8986\u76d6\u65ad\u5f00\uff0c\u56e0\u6b64\u4e0b\u9762\u7684\u6570\u5b57\u53ea\u4f5c\u4e3a\u89e3\u91ca\u6587\u4ef6\u7684\u5bf9\u7167\u3002<\/p>\n

\u5148\u628a\u4e24\u4e2a\u6b63\u786e\u7684\u7ed3\u679c\u653e\u5230\u540c\u4e00\u6761\u5750\u6807\u8f74<\/h2>\n

\u4e09\u5341\u4e00\u78b1\u57fa\u8bbe\u7f6e\u6210\u529f\u9000\u51fa\uff0c\u6700\u7ec8 FASTA \u91cc\u4e24\u6761\u5206\u522b\u4e3a\u4e00\u4e07\u4e94\u5343\u96f6\u4e03\u5341\u4e03\u548c\u4e00\u4e07\u56db\u5343\u96f6\u4e03\u5341\u4e09\u78b1\u57fa\uff0c\u603b\u8ba1\u4e8c\u4e07\u4e5d\u5343\u4e00\u767e\u4e94\u5341\u3002\u5c06\u6bcf\u6761\u5e8f\u5217\u7684\u6b63\u5411\u53ca\u53cd\u5411\u4e92\u8865\u90fd\u653e\u5230\u5df2\u77e5\u6a21\u677f\u4e0a\u627e\u7cbe\u786e\u5339\u914d\uff0c\u4e24\u6761\u90fd\u80fd\u9010\u5b57\u627e\u5230\u3002\u4e24\u6761\u8986\u76d6\u7684\u6a21\u677f\u5750\u6807\u53d6\u5e76\u96c6\u540e\u53ea\u5269\u4e8c\u4e07\u4e09\u5343\u96f6\u4e5d\u5341\u4e00\uff0c\u5dee\u989d\u662f\u516d\u5343\u96f6\u4e94\u5341\u4e5d\u4e2a\u91cd\u590d\u8ba1\u7b97\u7684\u78b1\u57fa\u3002\u8fd9\u4e2a\u91cd\u590d\u91cf\u8fdc\u5927\u4e8e\u4e94\u5341\u516d\uff0c\u8bf4\u660e\u88ab\u4e24\u6761\u8f93\u51fa\u5171\u540c\u8868\u793a\u7684\u533a\u57df\u4e0d\u6b62\u8bbe\u8ba1\u7684\u90a3\u4e2a\u5c0f\u91cd\u590d\u3002\u4e0d\u80fd\u628a\u603b\u957f\u51cf\u53bb\u6a21\u677f\u957f\u5ea6\u5c31\u5f53\u6210\u57fa\u56e0\u7ec4\u91cc\u65b0\u51fa\u73b0\u7684\u91cd\u590d\u62f7\u8d1d\u3002<\/p>\n

\u628a\u56fe\u7a97\u53e3\u6539\u4e3a\u56db\u5341\u4e00\u65f6\uff0c\u6700\u7ec8\u4ecd\u662f\u4e24\u6761\uff0c\u6bcf\u6761\u6bd4\u4e0a\u4e00\u7ec4\u957f\u5341\u4e2a\u78b1\u57fa\uff0c\u603b\u957f\u589e\u52a0\u4e8c\u5341\uff0c\u4f46\u4e24\u6761\u5171\u540c\u8986\u76d6\u7684\u72ec\u7acb\u5750\u6807\u6570\u5b8c\u5168\u76f8\u540c\u3002\u4ee5\u771f\u5b9e\u6a21\u677f\u4f5c\u5206\u6bcd\uff0c\u4e24\u79cd\u8bbe\u7f6e\u90fd\u8fd8\u6709\u4e8c\u5341\u4e00\u4e2a\u4f4d\u7f6e\u6ca1\u6709\u88ab\u6700\u7ec8\u957f\u5e8f\u5217\u8986\u76d6\u3002\u54ea\u4e00\u7ec4\u66f4\u9002\u5408\u540e\u7eed\uff0c\u4e0d\u80fd\u53ea\u770b\u54ea\u4e00\u4e2a\u603b\u957f\u66f4\u5927\u3002\u82e5\u76ee\u7684\u662f\u95ee\u7279\u5b9a\u57fa\u56e0\u533a\u6bb5\u662f\u5426\u88ab\u8986\u76d6\uff0c\u53ef\u67e5\u72ec\u7acb\u5750\u6807\u5e76\u96c6\u3002\u82e5\u76ee\u7684\u662f\u95ee\u4e24\u4e2a\u8fdc\u7aef\u4f4d\u70b9\u80fd\u5426\u53ef\u9760\u8fde\u63a5\uff0c\u5c31\u8981\u627e\u7ba1\u7ebf\u91cc\u652f\u6301\u8be5\u8def\u5f84\u7684\u77ed\u8bfb\u6bb5\u914d\u5bf9\u53ca\u65b9\u5411\uff0c\u5fc5\u8981\u65f6\u518d\u7528\u72ec\u7acb\u957f\u8bfb\u6bb5\u8de8\u8fc7\u63a5\u5934\u3002\u8fd9\u91cc\u6ca1\u6709\u8f93\u5165\u4efb\u4f55\u771f\u5b9e\u957f\u8bfb\u6bb5\uff0c\u4e0d\u80fd\u628a\u91cd\u53e0\u6784\u5efa\u51fa\u7684\u4e24\u6761\u5e8f\u5217\u8ba1\u4e3a\u4e24\u4efd\u72ec\u7acb\u7684\u957f\u5206\u5b50\u8bc1\u636e\u3002<\/p>\n

\n\n\n\n\n\n\n\n
\u56fe\u7a97\u53e3<\/th>\n\u9636\u6bb5\u9000\u51fa<\/th>\n\u6587\u4ef6\u91cc\u7684\u5e8f\u5217\u957f\u5ea6<\/th>\n\u7cbe\u786e\u6bd4\u56de\u6a21\u677f\u7684\u72ec\u7acb\u78b1\u57fa<\/th>\n<\/tr>\n<\/thead>\n
31<\/td>\n\u6210\u529f<\/td>\n15077\u300114073<\/td>\n23091<\/td>\n<\/tr>\n
41<\/td>\n\u6210\u529f<\/td>\n15087\u300114083<\/td>\n23091<\/td>\n<\/tr>\n
71<\/td>\n\u9000\u51fa\u7801 1<\/td>\n\u4e2d\u95f4\u6587\u4ef6\u4e00\u6761 23091\uff0c\u6700\u7ec8\u6b65\u9aa4\u672a\u5b8c\u6210<\/td>\n23091\uff0c\u6309\u4e2d\u95f4\u5e8f\u5217\u8ba1\u7b97<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u4e24\u6761\u957f\u5e8f\u5217\u5982\u4f55\u8ba1\u6570\u3002\u540c\u4e00\u4efd
\u540c\u4e00\u4efd 4400 \u5bf9\u77ed\u8bfb\u6bb5\uff0c\u7528\u4e09\u4e2a\u56fe\u7a97\u53e3\u751f\u6210 super-read \u5e76\u6bd4\u5bf9\u5df2\u77e5\u6a21\u677f\u3002<\/figcaption><\/figure>\n

\u62a5\u544a\u4e2d\u518d\u7ed9\u51fa\u6700\u957f\u4e00\u6761\u5e8f\u5217\u5360\u6a21\u677f\u7684\u591a\u5c11\uff0c\u4e5f\u6709\u52a9\u4e8e\u53d1\u73b0\u5206\u6bcd\u9519\u8bef\u3002\u67d0\u4e9b\u5de5\u5177\u4f1a\u628a\u4ea7\u7269\u5f53\u4f5c\u65b0\u7684\u8f93\u5165\u96c6\u5408\u91cd\u65b0\u7edf\u8ba1\u8986\u76d6\u6df1\u5ea6\uff0c\u5982\u679c\u628a\u5df2\u7ecf\u5408\u5e76\u7684\u5171\u8bc6\u5e8f\u5217\u6bcf\u6761\u53ea\u8d4b\u4e00\u7968\uff0c\u7ed3\u679c\u63cf\u8ff0\u7684\u662f\u8def\u5f84\u7684\u6761\u6570\uff0c\u65e0\u6cd5\u6062\u590d\u539f\u6765\u6709\u591a\u5c11\u7247\u6bb5\u63d0\u4f9b\u4e86\u652f\u6301\u3002<\/p>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u76ee\u5f55\u91cc\u5df2\u6709 FASTA\uff0c\u4ecd\u9700\u68c0\u67e5\u662f\u54ea\u4e00\u6b65\u5199\u7684<\/h2>\n
DATA\r\nPE= pe 300 18 \/data\/R1.fastq \/data\/R2.fastq\r\nEND\r\nPARAMETERS\r\nGRAPH_KMER_SIZE=31\r\nSTOP_AFTER_SUPERREADS=1\r\nUSE_LINKING_MATES=0\r\nNUM_THREADS=2\r\nJF_SIZE=1000000\r\nEND\r\n<\/code><\/pre>\n
\u8fd0\u884c\u65f6\u5148\u7528 MaSuRCA \u8bfb\u53d6\u4e0a\u8ff0\u914d\u7f6e\u751f\u6210\u7ec4\u88c5\u811a\u672c\uff0c\u518d\u5728\u76f8\u5e94\u76ee\u5f55\u6267\u884c\u811a\u672c\uff0c\u6bcf\u7ec4\u53c2\u6570\u7528\u72ec\u7acb\u76ee\u5f55\u3002\u4e03\u5341\u4e00\u78b1\u57fa\u90a3\u8f6e\u524d\u6bb5\u4e5f\u6210\u529f\u751f\u6210\u4e86\u6bd4\u5bf9\u653e\u7f6e\u8bb0\u5f55\u548c\u4e00\u6761\u4e2d\u95f4\u5e8f\u5217\uff0c\u957f\u5ea6\u6b63\u597d\u662f\u4e8c\u4e07\u4e09\u5343\u96f6\u4e5d\u5341\u4e00\u3002\u7ee7\u7eed\u8fd0\u884c\u65f6\uff0c\u540e\u9762\u7684\u7ea6\u7b80\u7a0b\u5e8f\u7ed9\u51fa\u7684\u4e34\u65f6\u8f93\u51fa\u662f\u7a7a\u6587\u4ef6\uff0c\u9a71\u52a8\u811a\u672c\u8981\u6c42\u8be5\u6587\u4ef6\u81f3\u5c11\u6709\u4e00\u4e2a\u5b57\u8282\uff0c\u56e0\u6b64\u8fd4\u56de\u5931\u8d25\u3002\u6211\u4eec\u76f4\u63a5\u68c0\u67e5\u5177\u4f53\u65e5\u5fd7\u3001\u9636\u6bb5\u6807\u8bb0\u548c\u5b9e\u9645\u6587\u4ef6\u8def\u5f84\u3002\u6b64\u5904\u53ef\u4ee5\u9a8c\u8bc1\u4e2d\u95f4\u90a3\u6761\u5e8f\u5217\u4e0e\u6a21\u677f\u76f8\u7b26\uff0c\u4e0d\u80fd\u628a\u8fd9\u8f6e\u63cf\u8ff0\u6210\u6210\u529f\u4ea7\u51fa\u6700\u7ec8 super-read\uff0c\u66f4\u4e0d\u80fd\u62ff\u5b83\u5f53\u4f5c\u5b8c\u6574\u57fa\u56e0\u7ec4\u7ec4\u88c5\u3002\u672c\u6b21\u4f7f\u7528\u7684 MaSuRCA 4.1.4<\/a><\/p>\n
\u6700\u5bb9\u6613\u8bef\u5224\u7684\u60c5\u51b5\u662f\uff0c\u53ea\u7edf\u8ba1\u76ee\u5f55\u91cc\u6700\u5927\u7684 FASTA\uff0c\u6216\u53ea\u67e5\u6587\u4ef6\u540d\u4e2d\u6709\u6ca1\u6709\u6210\u529f\u6807\u8bb0\u3002\u7a0b\u5e8f\u5728\u4e0d\u540c\u5b50\u6b65\u9aa4\u90fd\u53ef\u80fd\u7559\u4e0b\u6210\u529f\u6807\u8bb0\uff0c\u65e9\u671f\u6210\u529f\u4e0e\u665a\u671f\u5931\u8d25\u53ef\u4ee5\u540c\u65f6\u5b58\u5728\u3002\u6838\u9a8c\u65f6\u5148\u628a\u4e3b\u7a0b\u5e8f\u9000\u51fa\u72b6\u6001\u5199\u8fdb\u8868\uff0c\u518d\u5206\u522b\u767b\u8bb0\u8fdb\u5165\u7edf\u8ba1\u7684\u6587\u4ef6\u8def\u5f84\u548c\u8ba1\u7b97\u53e3\u5f84\u3002\u5bf9\u4e8e\u5dee\u5f02\u6700\u5927\u7684\u90a3\u4e00\u8f6e\uff0c\u91cd\u65b0\u7b97\u4e00\u904d\u72ec\u7acb\u57fa\u56e0\u7ec4\u5750\u6807\u8986\u76d6\uff0c\u518d\u6bd4\u8f83\u8bfb\u6bb5\u653e\u7f6e\u8bb0\u5f55\u7684\u884c\u6570\u3002\u672c\u4f8b\u4e09\u8f6e\u7684\u653e\u7f6e\u6587\u4ef6\u90fd\u662f\u516b\u5343\u4e03\u767e\u4e5d\u5341\u516b\u884c\uff0c\u8fd9\u4e0d\u4ee3\u8868\u5b83\u4eec\u6784\u51fa\u4e86\u76f8\u540c\u6570\u91cf\u7684\u6700\u7ec8\u957f\u5e8f\u5217\uff0c\u4e5f\u4e0d\u80fd\u628a\u884c\u6570\u76f4\u63a5\u6362\u7b97\u6210\u5b8c\u6574\u4fdd\u7559\u7684\u914d\u5bf9\u6570\u3002<\/p>\n
\u771f\u5b9e\u6570\u636e\u7f3a\u5c11\u5df2\u77e5\u6a21\u677f\u65f6\uff0c\u53ef\u4ee5\u6362\u6210\u4e24\u9879\u5916\u90e8\u8bc1\u636e\u3002\u7b2c\u4e00\u9879\u4ece\u672a\u53c2\u4e0e\u7ec4\u88c5\u7684\u9ad8\u51c6\u786e\u8bfb\u6bb5\u4e2d\u62bd\u53d6\u8de8\u63a5\u5206\u5b50\uff0c\u68c0\u67e5\u957f\u5e8f\u5217\u4e2d\u5019\u9009\u8fde\u63a5\u70b9\u7684\u4e24\u4fa7\u662f\u5426\u90fd\u80fd\u552f\u4e00\u951a\u5b9a\u3002\u7b2c\u4e8c\u9879\u5bf9\u65b0\u5e8f\u5217\u95f4\u505a\u76f8\u4e92\u6bd4\u5bf9\uff0c\u753b\u51fa\u957f\u8303\u56f4\u91cd\u53e0\u7684\u7247\u6bb5\u518d\u7edf\u8ba1\u603b\u957f\u5ea6\u3002\u62ff\u5230\u8fd9\u4e24\u4efd\u7ed3\u679c\uff0c\u624d\u80fd\u56de\u7b54\u4e00\u6bb5\u53d8\u957f\u7684 super-read \u4ee3\u8868\u66f4\u591a\u5df2\u9a8c\u8bc1\u7684\u8fde\u63a5\uff0c\u8fd8\u662f\u53ea\u628a\u4e24\u6761\u8f93\u51fa\u5171\u6709\u7684\u533a\u95f4\u7565\u5fae\u5ef6\u957f\u4e86\u3002\u539f\u59cb\u53cc\u7aef\u8bfb\u6bb5\u3001\u914d\u5bf9\u4fe1\u606f\u4e0e\u5b9e\u9645\u6784\u56fe\u53c2\u6570\u8fd9\u4e09\u4efd\u6750\u6599\u90fd\u503c\u5f97\u4e0e\u6700\u7ec8\u4ea7\u7269\u4e00\u8d77\u4fdd\u7559\u3002<\/p>\n
\u5f53\u591a\u4efd\u6837\u672c\u9700\u8981\u540c\u65f6\u4fdd\u5b58\u539f\u59cb\u53cc\u7aef\u6587\u5e93\u3001\u591a\u4e2a\u56fe\u7a97\u53e3\u7684\u4e2d\u95f4\u5e8f\u5217\u4e0e\u72ec\u7acb\u9a8c\u8bc1\u7ed3\u679c\u65f6\uff0c\u53ef\u4ee5\u4f7f\u7528\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u5f00\u5c55\u6279\u91cf\u7ec4\u88c5\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u5b9e\u8dd1 MaSuRCA \u7684 super-read \u751f\u6210\u9636\u6bb5\uff0c\u6bd4\u8f83\u4e09\u7ec4 k \u503c\uff0c\u533a\u5206\u91cd\u590d\u8868\u793a\u7684\u5750\u6807\u3001\u4e2d\u95f4\u5e8f\u5217\u548c\u6b63\u5f0f\u4ea7\u7269\u3002<\/p>\n","protected":false},"author":1,"featured_media":71235,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[1051,1053,572,1052],"class_list":["post-71236","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-masurca","tag-super-read","tag-572","tag-1052"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/masurca-superreads-kmer-overlap-distinct-reference-bases-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/71236","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=71236"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/71236\/revisions"}],"predecessor-version":[{"id":71237,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/71236\/revisions\/71237"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/71235"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=71236"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=71236"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=71236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}