{"id":71322,"date":"2026-11-15T00:25:44","date_gmt":"2026-11-14T16:25:44","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=71322"},"modified":"2026-11-15T00:25:44","modified_gmt":"2026-11-14T16:25:44","slug":"medaka-consensus-filled-gaps-vs-masked-n-coverage","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/medaka-consensus-filled-gaps-vs-masked-n-coverage\/","title":{"rendered":"Medaka \u5b9e\u8dd1\uff5c\u5171\u8bc6\u5e8f\u5217\u6709 3600 \u4e2a\u78b1\u57fa\uff0c\u6a21\u578b\u771f\u6b63\u9884\u6d4b\u4e86\u591a\u5c11\u4e2a\uff1f"},"content":{"rendered":"<div class=\"ysx-method-guide\" style=\"max-width:820px;min-width:0;margin:0 auto;color:#26374a;line-height:1.86;overflow-wrap:anywhere\">\n<p>\u4e00\u4efd\u629b\u5149\u540e\u7684 FASTA \u4ece\u5934\u5230\u5c3e\u5168\u662f\u6b63\u5e38\u78b1\u57fa\uff0c\u770b\u4e0d\u5230\u4e00\u4e2a N\uff0c\u5e38\u8ba9\u4eba\u4ee5\u4e3a\u6bcf\u4e00\u4f4d\u90fd\u5f97\u5230\u4e86\u8bfb\u6bb5\u652f\u6301\u3002Medaka \u53ef\u4ee5\u5728\u6ca1\u6709\u9884\u6d4b\u7684\u5730\u65b9\u6cbf\u7528\u539f\u8349\u56fe\uff0c\u6240\u4ee5\u8f93\u51fa\u770b\u4f3c\u5b8c\u6574\u65f6\uff0c\u6700\u9700\u8981\u67e5\u7684\u6587\u4ef6\u5f80\u5f80\u662f\u65c1\u8fb9\u90a3\u4efd\u7f3a\u53e3\u533a\u95f4\u8868\u3002\u6211\u4eec\u8ba9\u8f6f\u4ef6\u7684\u795e\u7ecf\u7f51\u7edc\u5b9e\u9645\u9884\u6d4b\u4e00\u6b21\uff0c\u518d\u4ece\u5b8c\u5168\u76f8\u540c\u7684\u9884\u6d4b\u6587\u4ef6\u751f\u6210\u4e09\u4efd\u8f93\u51fa\u3002\u5728\u6ca1\u6709\u6d4b\u5230\u7684\u540c\u4e00\u4e2a\u4f4d\u7f6e\uff0c\u6700\u7ec8\u53ef\u4ee5\u5f97\u5230 A\u3001N\uff0c\u6216\u8005\u6839\u672c\u6ca1\u6709\u8be5\u4f4d\u7f6e\u3002\u533a\u522b\u6765\u81ea\u751f\u6210\u5171\u8bc6\u65f6\u5982\u4f55\u8868\u793a\u7f3a\u53e3\u3002<a style=\"color:#5d558d;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/nanoporetech\/medaka\/blob\/v2.2.2\/README.md\">Medaka 2.2.2 \u7684\u5de5\u4f5c\u6d41\u7a0b<\/a><\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u628a\u4e00\u4e2a\u9519\u8bef\u653e\u5728\u6709\u8bfb\u6bb5\u5904\uff0c\u53e6\u4e00\u4e2a\u653e\u5728\u65e0\u4eba\u8986\u76d6\u5904<\/h2>\n<p>\u751f\u6210\u4e00\u6761\u4e09\u5343\u516d\u767e\u78b1\u57fa\u7684\u4eba\u5de5\u771f\u503c\uff0c\u5728\u7b2c\u4e00\u5343\u4e94\u767e\u4f4d\u548c\u7b2c\u4e09\u5343\u4e00\u767e\u4f4d\u5404\u628a\u539f\u6765\u7684 T \u6539\u6210 A\uff0c\u5f97\u5230\u5f85\u629b\u5149\u8349\u56fe\u3002\u4ece\u771f\u503c\u800c\u975e\u8349\u56fe\u53d6\u56db\u5341\u56db\u6761\u957f\u4e00\u5343\u4e8c\u767e\u78b1\u57fa\u7684\u8bfb\u6bb5\uff0c\u6b63\u53cd\u5404\u4e8c\u5341\u4e8c\u6761\uff0c\u6240\u6709\u8bfb\u6bb5\u90fd\u843d\u5728\u8349\u56fe\u4e2d\u90e8\u3002\u6700\u5de6\u8fb9\u7684\u8bfb\u6bb5\u4ece\u96f6\u57fa\u7b2c\u56db\u767e\u4f4d\u5f00\u59cb\uff0c\u6700\u53f3\u7684\u8bfb\u6bb5\u672b\u7aef\u5230\u7b2c\u4e8c\u5343\u516d\u767e\u4f4d\u4e4b\u524d\u3002\u8fd9\u6837\u7b2c\u4e00\u4e2a\u9519\u8bef\u6709\u591a\u6761\u89c2\u6d4b\u7a7f\u8fc7\uff0c\u7b2c\u4e8c\u4e2a\u9519\u8bef\u8fde\u4e00\u6761\u4e5f\u6ca1\u6709\u3002\u6a21\u62df\u8bfb\u6bb5\u6545\u610f\u4e0d\u542b\u6d4b\u5e8f\u9519\u8bef\uff0c\u76ee\u7684\u5728\u4e8e\u9a8c\u8bc1\u6709\u8bc1\u636e\u548c\u65e0\u8bc1\u636e\u4f4d\u7f6e\u7684\u8f93\u51fa\u6765\u6e90\u3002<\/p>\n<p>\u5148\u7528 minimap2 \u628a\u8bfb\u6bb5\u6bd4\u56de\u8349\u56fe\uff0c\u6392\u5e8f\u5efa\u7d22\u5f15\uff0c\u518d\u7528\u5b89\u88c5\u7684 Medaka 2.2.2 \u5728\u4e2d\u592e\u5904\u7406\u5668\u4e0a\u6267\u884c\u5b9e\u9645\u795e\u7ecf\u7f51\u7edc\u63a8\u7406\u3002\u663e\u5f0f\u4f7f\u7528\u8f6f\u4ef6\u9644\u5e26\u7684\u7eb3\u7c73\u5b54\u8d85\u9ad8\u51c6\u786e\u5ea6\u78b1\u57fa\u8bc6\u522b\u6a21\u5f0f SUP \u5bf9\u5e94\u7684\u629b\u5149\u6a21\u578b\uff0c\u7248\u672c\u4e3a\u4e94\u70b9\u4e8c\u3002\u8fd9\u91cc\u7684\u8bfb\u6bb5\u6765\u81ea\u6a21\u62df\u5668\uff0c\u5e76\u6ca1\u6709\u7ecf\u8fc7\u771f\u5b9e\u78b1\u57fa\u8bc6\u522b\u6a21\u578b\u3002\u8fd9\u79cd\u8bbe\u7f6e\u5141\u8bb8\u68c0\u9a8c\u7a0b\u5e8f\u5728\u5212\u5b9a\u7684\u8986\u76d6\u8303\u56f4\u5185\u5982\u4f55\u751f\u6210\u548c\u62fc\u63a5\u9884\u6d4b\uff0c\u4e0d\u80fd\u7528\u6765\u8bc4\u4f30\u8be5\u6a21\u578b\u5904\u7406\u67d0\u4e00\u7c7b\u771f\u5b9e\u5316\u5b66\u6761\u4ef6\u7684\u51c6\u786e\u7387\u3002\u6b63\u5f0f\u9879\u76ee\u5e94\u6839\u636e\u5b9e\u9645\u78b1\u57fa\u8bc6\u522b\u6a21\u578b\u548c\u6587\u5e93\u9009\u62e9\u76f8\u5e94\u7684\u629b\u5149\u6a21\u578b\uff0c\u5e76\u628a\u4e8c\u8005\u5b8c\u6574\u540d\u79f0\u5199\u8fdb\u65e5\u5fd7\u3002<a style=\"color:#5d558d;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/nanoporetech\/medaka\/blob\/v2.2.2\/medaka\/options.py\">Medaka \u7684\u6a21\u578b\u5bf9\u5e94\u8868<\/a><\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u4ece\u4e00\u4e2a\u9884\u6d4b\u6587\u4ef6\u751f\u6210\u4e09\u79cd\u53ef\u6838\u5bf9\u7684\u8f93\u51fa<\/h2>\n<p>\u795e\u7ecf\u7f51\u7edc\u65e5\u5fd7\u663e\u793a\uff0c\u8bf7\u6c42\u9884\u6d4b\u4ece\u96f6\u5230\u4e09\u5343\u516d\u767e\u7684\u6574\u6761\u8349\u56fe\uff0c\u5b9e\u9645\u6709\u5806\u53e0\u89c2\u6d4b\u7684\u4f4d\u7f6e\u53ea\u4ece\u56db\u767e\u5230\u4e8c\u5343\u4e94\u767e\u4e5d\u5341\u4e5d\uff0c\u4e24\u7aef\u5747\u4e3a\u96f6\u57fa\u4f4d\u7f6e\u3002\u5c06\u540e\u8005\u5199\u6210\u5de6\u95ed\u53f3\u5f00\u533a\u95f4\u5c31\u662f\u4ece\u56db\u767e\u5230\u4e8c\u5343\u516d\u767e\uff0c\u5171\u4e8c\u5343\u4e8c\u767e\u4e2a\u78b1\u57fa\u3002\u968f\u540e\u4e09\u8f6e\u751f\u6210\u5171\u8bc6\u90fd\u8bfb\u53d6\u540c\u4e00\u4efd\u9884\u6d4b\u6587\u4ef6\uff0c\u6ca1\u6709\u91cd\u65b0\u6bd4\u5bf9\u6216\u91cd\u65b0\u8fd0\u884c\u795e\u7ecf\u7f51\u7edc\u3002\u8fd9\u6837\u4efb\u4f55\u5dee\u5f02\u90fd\u53ef\u4ee5\u8ffd\u5230\u8f93\u51fa\u9636\u6bb5\u3002\u9605\u8bfb\u65e5\u5fd7\u65f6\u8981\u6ce8\u610f\u672b\u4f4d\u7684\u4e66\u5199\u7ea6\u5b9a\uff0c\u5426\u5219\u5f88\u5bb9\u6613\u628a\u6700\u540e\u4e00\u4e2a\u6709\u8bfb\u6bb5\u7684\u78b1\u57fa\u5212\u8fdb\u7f3a\u53e3\u3002<\/p>\n<p>\u9ed8\u8ba4\u751f\u6210\u5b8c\u6574\u5e8f\u5217\uff0c\u7ed3\u679c\u957f\u4e09\u5343\u516d\u767e\uff0c\u7b2c\u4e00\u4e2a\u9519\u8bef\u4fee\u56de T\uff0c\u7b2c\u4e8c\u4e2a\u4f4d\u7f6e\u5374\u4ecd\u4e3a\u8349\u56fe\u9519\u8bef\u7684 A\u3002\u5bf9\u7167\u771f\u503c\uff0c\u5176\u4f59\u78b1\u57fa\u5168\u90e8\u76f8\u540c\u3002\u5c06\u7f3a\u53e3\u586b\u5145\u5b57\u7b26\u6539\u4e3a N\uff0c\u518d\u5f97\u5230\u4e00\u6761\u540c\u6837\u957f\u7684\u5e8f\u5217\uff0c\u5176\u4e2d\u6070\u6709\u4e00\u5343\u56db\u767e\u4e2a N\uff0c\u539f\u6765\u7b2c\u4e09\u5343\u4e00\u767e\u4f4d\u6240\u5728\u7684\u533a\u57df\u4e5f\u5728\u5176\u4e2d\u3002\u5173\u95ed\u7f3a\u53e3\u586b\u5145\u53ea\u8f93\u51fa\u6709\u9884\u6d4b\u7684\u7247\u6bb5\uff0c\u5f97\u5230\u4e00\u6761\u4e8c\u5343\u4e8c\u767e\u78b1\u57fa\u7684\u5e8f\u5217\uff0c\u4e0e\u771f\u503c\u4e2d\u90e8\u9010\u5b57\u76f8\u540c\u3002\u77ed\u6587\u4ef6\u6807\u9898\u9644\u6709\u539f\u8349\u56fe\u533a\u95f4\uff0c\u53ef\u4ee5\u5c06\u5c40\u90e8\u4f4d\u7f6e\u6362\u56de\u539f\u5750\u6807\u3002\u628a\u8fd9\u4e09\u6761 FASTA \u7684\u957f\u5ea6\u76f4\u63a5\u753b\u6210\u6a21\u578b\u6548\u679c\u6392\u540d\uff0c\u4f1a\u7ed9\u540c\u4e00\u6b21\u63a8\u7406\u7f16\u9020\u51fa\u4e09\u4e2a\u4e0d\u540c\u7684\u51c6\u786e\u7387\u3002<\/p>\n<div class=\"guide-table\" style=\"max-width:100%;overflow-x:auto;margin:18px 0\">\n<table style=\"display:table;width:100%;max-width:100%;min-width:0;table-layout:fixed;border-collapse:collapse;font-size:.92em;line-height:1.6\">\n<thead>\n<tr>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#f6f2ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u4f7f\u7528\u540c\u4e00\u4efd\u9884\u6d4b<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#f6f2ff;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">FASTA \u957f\u5ea6<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#f6f2ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u539f\u7b2c 1500 \u4f4d<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#f6f2ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u539f\u7b2c 3100 \u4f4d<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u9ed8\u8ba4\u7528\u8349\u56fe\u586b\u8865<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">3600<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">T\uff0c\u7ea0\u6b63<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">A\uff0c\u6cbf\u7528\u8349\u56fe<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u7f3a\u53e3\u586b N<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">3600<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">T\uff0c\u7ea0\u6b63<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">N<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u53ea\u4fdd\u7559\u6709\u9884\u6d4b\u7684\u7247\u6bb5<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">2200<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">T\uff0c\u4f4d\u4e8e\u8f93\u51fa\u7b2c 1100 \u4f4d<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u6ca1\u6709\u5bf9\u5e94\u78b1\u57fa<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<figure class=\"guide-figure\" style=\"margin:24px 0 28px\"><img loading=\"lazy\" decoding=\"async\" src=\"\/wordpress\/wp-content\/uploads\/2026\/11\/medaka-consensus-filled-gaps-vs-masked-n-coverage-guide.png\" alt=\"\u5b8c\u6574\u7684 FASTA \u4ece\u54ea\u91cc\u6765\u3002\u540c\u4e00\u4e2a\u795e\u7ecf\u7f51\u7edc\u9884\u6d4b\u6587\u4ef6\u751f\u6210\u4e09\u79cd\u5171\u8bc6\uff0c\u68c0\u67e5\u8986\u76d6\u5185\u5916\u7684\u4e24\u4e2a\u5df2\u77e5\u9519\u8bef\u3002\u9ed8\u8ba4\u56de\u586b\uff0c3600 bp \u00b7 \u65e0 N\uff0c\u7b2c 1500 \u4f4d\u4fee\u56de T\uff0c\u7b2c 3100 \u4f4d\u4ecd\u4fdd\u7559\u8349\u56fe\u7684 A\u3002\u7528 N \u5c4f\u853d\uff0c1400 \u4e2a N\uff0c\u4e24\u4e2a\u7f3a\u53e3\u5206\u522b\u957f 400 \u548c 1000 bp\uff0c\u6b63\u597d\u5bf9\u4e0a\u3002\u53ea\u7559\u9884\u6d4b\u5757\uff0c2200 bp\uff0c\u4e2d\u6bb5\u4e0e\u771f\u503c\u76f8\u540c\uff0c\u539f\u7b2c 3100 \u4f4d\u5df2\u4e0d\u5728\u8f93\u51fa\u91cc\u3002\" width=\"1200\" height=\"840\" style=\"display:block;width:100%;height:auto;border-radius:10px\"><figcaption style=\"font-size:14px;line-height:1.6;margin:9px 2px 0;color:#475569\">\u540c\u4e00\u4e2a\u795e\u7ecf\u7f51\u7edc\u9884\u6d4b\u6587\u4ef6\u751f\u6210\u4e09\u79cd\u5171\u8bc6\uff0c\u68c0\u67e5\u8986\u76d6\u5185\u5916\u7684\u4e24\u4e2a\u5df2\u77e5\u9519\u8bef\u3002<\/figcaption><\/figure>\n<p>\u82e5\u628a\u629b\u5149\u7ed3\u679c\u76f4\u63a5\u9001\u53bb\u6ce8\u91ca\uff0c\u4fdd\u7559\u8349\u56fe\u7684\u7248\u672c\u6709\u5229\u4e8e\u7ef4\u6301\u5e8f\u5217\u540d\u79f0\u4e0e\u539f\u6709\u5750\u6807\uff0c\u5c4f\u853d\u7248\u5219\u80fd\u63d0\u9192\u6ce8\u91ca\u4eba\u5458\u67d0\u4e2a\u5916\u663e\u5b50\u4e2d\u7684\u5b57\u6bcd\u7f3a\u5c11\u65b0\u589e\u8bc1\u636e\u3002\u6b63\u5f0f\u6570\u636e\u82e5\u53d1\u751f\u63d2\u5165\u6216\u5220\u9664\uff0c\u6700\u7ec8\u5171\u8bc6\u7684\u5750\u6807\u8fd8\u4f1a\u76f8\u5bf9\u8349\u56fe\u5e73\u79fb\u3002\u7f3a\u53e3\u6587\u4ef6\u660e\u786e\u4ee5\u8349\u56fe\u4e3a\u5750\u6807\u7cfb\uff0c\u53e0\u52a0\u5230\u65b0\u7248\u6ce8\u91ca\u524d\u5e94\u5efa\u7acb\u4e24\u7248\u4e4b\u95f4\u7684\u6620\u5c04\u3002\u672c\u4f8b\u53ea\u6709\u7b49\u957f\u66ff\u6362\uff0c\u7279\u610f\u8ba9\u5750\u6807\u53d8\u6362\u4e0d\u5e72\u6270\u5bf9\u7f3a\u53e3\u6765\u6e90\u7684\u5224\u65ad\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u5148\u7528 BED \u5bf9\u8d26\uff0c\u518d\u628a\u533a\u95f4\u4ea4\u7ed9\u4e0b\u6e38<\/h2>\n<pre style=\"max-width:100%;box-sizing:border-box;overflow-x:auto;margin:18px 0;padding:14px 16px;background:#f3f6fa;color:#1a293c;border:1px solid #d8e1ea;border-radius:8px;line-height:1.55;white-space:pre;overflow-wrap:normal;word-break:normal\"><code class=\"language-bash\">medaka inference input.bam inference.hdf --model r1041_e82_400bps_sup_v5.2.0 --threads 2 --batch_size 4\r\nmedaka sequence inference.hdf draft.fa filled.fa\r\nmedaka sequence inference.hdf draft.fa masked.fa --fill_char N\r\nmedaka sequence inference.hdf draft.fa blocks.fa --no-fillgaps\r\ncat filled.fa.gaps_in_draft_coords.bed\r\n<\/code><\/pre>\n<p>\u9ed8\u8ba4\u8f93\u51fa\u4e0e\u586b N \u8f93\u51fa\u90fd\u9644\u5e26\u8349\u56fe\u5750\u6807\u4e0b\u7684\u7f3a\u53e3\u6587\u4ef6\u3002\u4e24\u4efd\u5185\u5bb9\u4e00\u81f4\uff0c\u5206\u522b\u662f\u4ece\u96f6\u5230\u56db\u767e\u3001\u4ece\u4e8c\u5343\u516d\u767e\u5230\u4e09\u5343\u516d\u767e\uff0c\u957f\u5ea6\u76f8\u52a0\u4e00\u5343\u56db\u767e\uff0c\u6070\u597d\u7b49\u4e8e\u586b N \u8f93\u51fa\u7684 N \u6570\u3002\u5c06\u4e24\u4e2a\u7f3a\u53e3\u4e0e\u4e2d\u592e\u7684\u4e8c\u5343\u4e8c\u767e\u76f8\u52a0\uff0c\u6b63\u597d\u6062\u590d\u6574\u6761\u8349\u56fe\u7684\u4e09\u5343\u516d\u767e\u3002\u53ea\u4fdd\u7559\u9884\u6d4b\u7247\u6bb5\u65f6\uff0c\u6211\u4eec\u5728\u8be5\u6b21\u72ec\u7acb\u8f93\u51fa\u8def\u5f84\u4e2d\u6ca1\u6709\u5f97\u5230\u7f3a\u53e3 BED\uff0c\u56e0\u6b64\u9700\u8981\u5750\u6807\u6620\u5c04\u65f6\uff0c\u5e94\u4fdd\u7559\u751f\u6210\u5b8c\u6574\u5e8f\u5217\u65f6\u5bf9\u5e94\u7684\u533a\u95f4\u6587\u4ef6\u3002\u771f\u5b9e\u57fa\u56e0\u7ec4\u8349\u56fe\u672c\u8eab\u53ef\u80fd\u5df2\u7ecf\u5305\u542b N\uff0c\u4e0d\u80fd\u628a\u6587\u4ef6\u91cc\u6240\u6709 N \u7684\u6570\u76ee\u76f4\u63a5\u5f53\u6210\u65b0\u589e\u7f3a\u53e3\uff0c\u6700\u597d\u5206\u522b\u7edf\u8ba1\u539f\u6709\u6a21\u7cca\u78b1\u57fa\u4e0e\u6b64\u6b21\u672a\u9884\u6d4b\u533a\u95f4\u3002<a style=\"color:#5d558d;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/nanoporetech\/medaka\/blob\/v2.2.2\/medaka\/stitch.py\">Medaka \u5171\u8bc6\u62fc\u63a5\u548c\u7f3a\u53e3\u5904\u7406\u5b9e\u73b0<\/a><\/p>\n<p>\u82e5\u76ee\u6807\u57fa\u56e0\u7b2c\u4e00\u4e2a\u53ef\u7591\u66ff\u6362\u4f4d\u4e8e\u88ab\u8bfb\u6bb5\u8986\u76d6\u7684\u533a\u57df\uff0c\u4ecd\u7136\u8981\u68c0\u67e5\u5b9e\u9645\u6df1\u5ea6\u3001\u4e24\u6761\u94fe\u7684\u652f\u6301\u548c\u6bd4\u5bf9\u4f4d\u7f6e\u662f\u5426\u552f\u4e00\u3002\u76f8\u90bb\u91cd\u590d\u53ef\u80fd\u628a\u540c\u4e00\u6279\u8bfb\u6bb5\u653e\u5230\u9519\u8bef\u62f7\u8d1d\u4e0a\uff0c\u9020\u6210\u770b\u4f3c\u6709\u9884\u6d4b\u5374\u7f3a\u5c11\u72ec\u7acb\u652f\u6301\u3002\u82e5\u53e6\u4e00\u4e2a\u4f4d\u70b9\u843d\u5728\u7f3a\u53e3\u5185\uff0c\u5373\u4f7f\u6700\u7ec8 FASTA \u7ed9\u51fa\u4e00\u4e2a\u660e\u786e\u5b57\u6bcd\uff0c\u4e5f\u53ea\u80fd\u8ffd\u6eaf\u5230\u539f\u8349\u56fe\u3002\u62a5\u544a\u65f6\u53ef\u4ee5\u628a\u4f4d\u70b9\u8868\u4e0e\u7f3a\u53e3\u76f8\u4ea4\uff0c\u4e3a\u6bcf\u6761\u8bb0\u5f55\u589e\u52a0\u4e00\u4e2a\u201c\u78b1\u57fa\u6765\u81ea\u629b\u5149\u533a\u8fd8\u662f\u8349\u56fe\u56de\u586b\u533a\u201d\u7684\u5b57\u6bb5\uff0c\u518d\u6309\u6765\u6e90\u89e3\u91ca\u5dee\u5f02\uff0c\u907f\u514d\u628a\u56de\u586b\u533a\u5199\u6210\u7ecf\u65b0\u6d4b\u5e8f\u8bc1\u5b9e\u7684\u7b49\u4f4d\u3002<\/p>\n<p>\u53e6\u4e00\u79cd\u5bb9\u6613\u5ffd\u7565\u7684\u60c5\u51b5\u662f\u7edf\u8ba1\u5206\u6bcd\u53d8\u5316\u3002\u53ea\u4fdd\u7559\u4e2d\u592e\u7247\u6bb5\u65f6\uff0c\u4e0e\u771f\u503c\u7684\u76f8\u4f3c\u5ea6\u5728\u672c\u4f8b\u4e2d\u8fbe\u5230\u5168\u957f\u7cbe\u786e\u5339\u914d\uff0c\u4f46\u5b83\u53ea\u8986\u76d6\u539f\u6a21\u677f\u7684\u4e00\u90e8\u5206\u3002\u6cbf\u7528\u8349\u56fe\u7684\u8f93\u51fa\u8986\u76d6\u5b8c\u6574\uff0c\u5374\u628a\u4e00\u4e2a\u5df2\u77e5\u9519\u8bef\u4e5f\u4fdd\u7559\u4e0b\u6765\u3002\u62a5\u544a\u5c40\u90e8\u78b1\u57fa\u4e00\u81f4\u7387\u65f6\u540c\u65f6\u7ed9\u51fa\u53c2\u4e0e\u6bd4\u8f83\u7684\u957f\u5ea6\u3001\u539f\u6a21\u677f\u957f\u5ea6\u548c\u672a\u8bc4\u4f30\u957f\u5ea6\uff0c\u5e76\u5c3d\u91cf\u4fdd\u7559\u4e00\u4efd\u5e26\u5750\u6807\u7684\u5c4f\u853d\u7248\u672c\uff0c\u624d\u80fd\u8ba9\u4e0b\u4e00\u4f4d\u4f7f\u7528\u8005\u6839\u636e\u81ea\u5df1\u7684\u7814\u7a76\u95ee\u9898\u9009\u62e9\u662f\u5426\u7eb3\u5165\u8fd9\u4e9b\u533a\u57df\u3002<\/p>\n<p class=\"guide-end\" style=\"margin-top:1.6em;padding-top:1.0em;border-top:1px solid #d7e0e9\">\u9700\u8981\u4e3a\u591a\u4efd\u7eb3\u7c73\u5b54\u7ec4\u88c5\u7edf\u4e00\u4fdd\u5b58\u8349\u56fe\u3001\u9884\u6d4b\u3001\u7f3a\u53e3\u533a\u95f4\u548c\u629b\u5149\u7ed3\u679c\u65f6\uff0c\u53ef\u4f7f\u7528<a data-ysx-event=\"contact_click\" style=\"color:#5d558d;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/www.biocloudservice.com\/wordpress\/compute-services\/shared-research-workspace\/\">\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u5f00\u5c55\u961f\u5217\u590d\u6838\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u5b9e\u8dd1 Medaka \u63a8\u7406\u4e0e\u4e09\u79cd\u5171\u8bc6\u8f93\u51fa\uff0c\u4ece BED \u7f3a\u53e3\u548c\u4e24\u5904\u4eba\u5de5\u66ff\u6362\u533a\u5206\u6a21\u578b\u9884\u6d4b\u3001\u539f\u8349\u56fe\u56de\u586b\u548c\u672a\u77e5\u78b1\u57fa\u3002<\/p>\n","protected":false},"author":1,"featured_media":71321,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[1058,1059,1060,415],"class_list":["post-71322","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-medaka","tag-1059","tag-1060","tag-415"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/medaka-consensus-filled-gaps-vs-masked-n-coverage-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/71322","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=71322"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/71322\/revisions"}],"predecessor-version":[{"id":71325,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/71322\/revisions\/71325"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/71321"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=71322"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=71322"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=71322"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}