{"id":71350,"date":"2026-11-15T00:35:16","date_gmt":"2026-11-14T16:35:16","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=71350"},"modified":"2026-11-15T00:35:16","modified_gmt":"2026-11-14T16:35:16","slug":"purge-dups-internal-terminal-get-seqs-23-n-gap","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/purge-dups-internal-terminal-get-seqs-23-n-gap\/","title":{"rendered":"purge_dups \u5b9e\u8dd1\uff5c\u540c\u662f\u5220\u9664 6000 bp\uff0c\u4e3a\u4f55\u4e3b\u5e8f\u5217\u91cc\u4f1a\u591a\u51fa 23 \u4e2a N\uff1f"},"content":{"rendered":"<div class=\"ysx-method-guide\" style=\"max-width:820px;min-width:0;margin:0 auto;color:#26374a;line-height:1.86;overflow-wrap:anywhere\">\n<p>\u7ec4\u88c5\u53bb\u5197\u4f59\u4e4b\u540e\uff0c\u6709\u4eba\u7528\u539f\u59cb\u603b\u957f\u51cf\u65b0\u603b\u957f\uff0c\u60f3\u5f97\u5230\u201c\u53bb\u6389\u4e86\u591a\u5c11\u91cd\u590d\u78b1\u57fa\u201d\uff0c\u7ed3\u679c\u603b\u5dee\u4e00\u70b9\u3002\u8fd8\u6709\u4eba\u53d1\u73b0\u4e00\u4e2a\u4f4d\u4e8e\u5019\u9009\u533a\u53f3\u8fb9\u7684\u57fa\u56e0\u5c45\u7136\u8de8\u8fc7\u4e86\u4e00\u4e32 N\u3002\u4e24\u79cd\u73b0\u8c61\u90fd\u8981\u56de\u5230\u53bb\u5197\u4f59\u64cd\u4f5c\u662f\u600e\u6837\u6539\u5199 FASTA \u7684\u3002\u6211\u4eec\u7528 purge_dups 1.2.6 \u5b98\u65b9\u7684\u5e8f\u5217\u63d0\u53d6\u7a0b\u5e8f\uff0c\u7ed9\u5b83\u4e00\u6bb5\u5185\u90e8\u5019\u9009\u548c\u4e00\u6bb5\u7aef\u90e8\u5019\u9009\uff0c\u5b9e\u9645\u8fd0\u884c\u4e09\u79cd\u65b9\u5f0f\u3002\u4e24\u4e2a\u5019\u9009\u533a\u95f4\u81ea\u59cb\u81f3\u7ec8\u76f8\u540c\uff0c\u6700\u7ec8\u4e3b\u6587\u4ef6\u7684\u6761\u6570\u3001\u540d\u79f0\u548c\u5750\u6807\u5374\u4e0d\u540c\u3002<a style=\"color:#744c6a;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/dfguan\/purge_dups\/blob\/v1.2.6\/README.md\">purge_dups \u4f5c\u8005\u7684\u7528\u6cd5\u8bf4\u660e<\/a><\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u628a\u8bc6\u522b\u5019\u9009\u548c\u5982\u4f55\u8f93\u51fa\u5019\u9009\u5206\u5f00\u68c0\u67e5<\/h2>\n<p>purge_dups \u5de5\u4f5c\u6d41\u53ef\u4ee5\u7ed3\u5408\u8bfb\u6bb5\u6df1\u5ea6\u4e0e\u7ec4\u88c5\u81ea\u6bd4\u5bf9\u6765\u5bfb\u627e\u53ef\u80fd\u91cd\u590d\u8868\u793a\u7684\u5355\u500d\u578b\u533a\u57df\uff0c\u4e4b\u540e\u518d\u6839\u636e\u5019\u9009\u533a\u95f4\u751f\u6210\u4fdd\u7559\u7684\u4e3b\u5e8f\u5217\u4e0e\u8f6c\u51fa\u7684\u7247\u6bb5\u3002\u8fd9\u91cc\u4e13\u95e8\u9a8c\u8bc1\u6700\u540e\u4e00\u9879\u64cd\u4f5c\uff0c\u8f93\u5165\u4f7f\u7528\u4eba\u4e3a\u6307\u5b9a\u7684 BED\uff0c\u6240\u4ee5\u5b9e\u9a8c\u4e0d\u5bf9\u8f6f\u4ef6\u53d1\u73b0\u91cd\u590d\u7684\u51c6\u786e\u7387\u4f5c\u4f30\u8ba1\u3002\u8fd9\u4e2a\u62c6\u5206\u5bf9\u771f\u5b9e\u9879\u76ee\u4e5f\u5f88\u5b9e\u7528\u3002\u4e00\u4e2a\u57fa\u56e0\u4ece\u4e3b\u7ec4\u88c5\u4e2d\u6d88\u5931\u65f6\uff0c\u5148\u786e\u8ba4\u5b83\u662f\u4e0d\u662f\u5df2\u7ecf\u51fa\u73b0\u5728\u5019\u9009 BED\uff0c\u7136\u540e\u68c0\u67e5\u5373\u4f7f\u7ed9\u5b9a\u540c\u4e00\u4efd\u5019\u9009\uff0c\u8f93\u51fa\u6a21\u5f0f\u672c\u8eab\u4f1a\u600e\u6837\u6539\u53d8\u8fd9\u6761\u5e8f\u5217\u3002<\/p>\n<p>\u6211\u4eec\u751f\u6210\u4e09\u6761\u65e0\u6a21\u7cca\u78b1\u57fa\u7684\u968f\u673a\u5e8f\u5217\uff0c\u5206\u522b\u957f\u4e94\u4e07\u3001\u4e09\u4e07\u4e94\u5343\u3001\u4e09\u4e07\u3002\u7b2c\u4e00\u6761\u7684\u5185\u90e8\u4ece\u96f6\u57fa\u4e8c\u4e07\u5230\u4e8c\u4e07\u516d\u5343\u6807\u4e3a\u5019\u9009\uff0c\u957f\u516d\u5343\u3002\u7b2c\u4e8c\u6761\u7684\u5f00\u5934\u4ece\u96f6\u5230\u4e94\u5343\u4e5f\u6807\u4e3a\u5019\u9009\u3002\u7b2c\u4e09\u6761\u6ca1\u6709\u4efb\u4f55\u5019\u9009\uff0c\u4f5c\u4e3a\u5e8f\u5217\u5b8c\u5168\u4e0d\u8be5\u53d8\u5316\u7684\u5bf9\u7167\u3002\u7531\u4e8e\u53ea\u6d4b\u8bd5\u533a\u95f4\u5904\u7406\uff0c\u8fd9\u4e9b\u968f\u673a\u5b57\u6bcd\u4e0d\u9700\u8981\u771f\u7684\u4e92\u4e3a\u91cd\u590d\u3002\u9a8c\u6536\u65f6\u5c06\u6240\u6709\u8f93\u51fa\u91cd\u65b0\u9010\u5b57\u5339\u914d\u5230\u539f\u4e09\u6761\u5e8f\u5217\uff0c\u6b63\u597d\u53ef\u4ee5\u7b97\u51fa\u54ea\u4e9b\u78b1\u57fa\u8f6c\u51fa\u4e86\u4e3b\u6587\u4ef6\u3001\u54ea\u4e9b\u662f\u771f\u6b63\u65b0\u589e\u7684\u5360\u4f4d\uff0c\u4ee5\u53ca\u91cd\u65b0\u547d\u540d\u4ee5\u540e\u6bcf\u6bb5\u5750\u6807\u5bf9\u5e94\u54ea\u91cc\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u4e3a\u4ec0\u4e48\u4fa7\u679d\u6587\u4ef6\u548c\u4e3b\u6587\u4ef6\u5fc5\u987b\u4e00\u8d77\u6570<\/h2>\n<p>\u5148\u4f7f\u7528\u53ea\u5904\u7406\u4e24\u7aef\u7684\u9009\u9879\u3002\u7ed3\u679c\u4e2d\u7aef\u90e8\u5019\u9009\u4e94\u5343\u4e2a\u78b1\u57fa\u8fdb\u5165\u53e6\u4e00\u4e2a FASTA\uff0c\u8be5\u539f\u7247\u6bb5\u5269\u4f59\u4e09\u4e07\u78b1\u57fa\u8fdb\u5165\u4e3b\u6587\u4ef6\u3002\u4e94\u4e07\u78b1\u57fa\u957f\u7684\u5185\u90e8\u5019\u9009\u6240\u5728\u5e8f\u5217\u6574\u6761\u4fdd\u7559\uff0c\u672a\u51fa\u73b0 N\u3002\u7b2c\u4e09\u6761\u4e09\u4e07\u78b1\u57fa\u5bf9\u7167\u4e5f\u5b8c\u6574\u4fdd\u7559\u3002\u6b64\u65f6\u4e3b\u6587\u4ef6\u5171\u5341\u4e00\u4e07\u4e2a\u5df2\u77e5\u78b1\u57fa\uff0c\u8f6c\u51fa\u6587\u4ef6\u4e94\u5343\uff0c\u4e8c\u8005\u52a0\u8d77\u6765\u6070\u4e3a\u8f93\u5165\u7684\u5341\u4e00\u4e07\u4e94\u5343\u3002\u6362\u53e5\u8bdd\u8bf4\uff0c\u5185\u90e8\u5019\u9009\u51fa\u73b0\u5728 BED\uff0c\u548c\u5185\u90e8\u5019\u9009\u786e\u5b9e\u88ab\u8fd9\u6b21\u8f93\u51fa\u5254\u9664\uff0c\u662f\u4e24\u4e2a\u9700\u8981\u5206\u522b\u6838\u5bf9\u7684\u4e8b\u5b9e\u3002<\/p>\n<p>\u9ed8\u8ba4\u8fd0\u884c\u65f6\u4e24\u6bb5\u5019\u9009\u90fd\u8f6c\u51fa\uff0c\u603b\u8ba1\u4e00\u4e07\u4e00\u5343\u78b1\u57fa\u3002\u5185\u90e8\u5019\u9009\u539f\u6765\u4e24\u4fa7\u7684\u5df2\u77e5\u5e8f\u5217\u5206\u522b\u957f\u4e8c\u4e07\u548c\u4e8c\u4e07\u56db\u5343\uff0c\u7a0b\u5e8f\u5c06\u5b83\u4eec\u7559\u5728\u540c\u4e00\u6761\u4e3b\u5e8f\u5217\u4e2d\uff0c\u4e2d\u95f4\u52a0\u5165\u4e8c\u5341\u4e09\u4e2a N\uff0c\u4e8e\u662f\u8fd9\u4e00\u6761\u53d8\u6210\u56db\u4e07\u56db\u5343\u96f6\u4e8c\u5341\u4e09\u3002\u7aef\u90e8\u5019\u9009\u53ea\u5f71\u54cd\u7b2c\u4e8c\u6761\u5f00\u5934\uff0c\u65e0\u987b\u5728\u4e24\u4e2a\u5269\u4f59\u7247\u6bb5\u4e4b\u95f4\u63d2\u6807\u8bb0\u3002\u5bf9\u7167\u4ecd\u7136\u6b63\u786e\u3002\u82e5\u53ea\u7528\u524d\u540e\u7684\u4e3b FASTA \u603b\u957f\u76f8\u51cf\uff0c\u4f1a\u5f97\u5230\u4e00\u4e07\u96f6\u4e5d\u767e\u4e03\u5341\u4e03\uff0c\u5c11\u7b97\u4e8c\u5341\u4e09\u3002\u6b63\u786e\u7684\u53bb\u5411\u53ef\u4ee5\u9010\u9879\u5bf9\u4e0a\uff0c\u539f\u8f93\u5165\u51cf\u771f\u6b63\u8f6c\u51fa\u7684\u78b1\u57fa\uff0c\u518d\u52a0\u65b0\u51fa\u73b0\u7684\u5360\u4f4d\u6570\uff0c\u7b49\u4e8e\u8f93\u51fa\u4e3b\u6587\u4ef6\u7684\u603b\u957f\u3002<a style=\"color:#744c6a;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/dfguan\/purge_dups\/blob\/v1.2.6\/src\/get_seqs.c\">\u8be5\u7248\u672c get_seqs \u7684\u533a\u95f4\u8f93\u51fa\u5b9e\u73b0<\/a><\/p>\n<div class=\"guide-table\" style=\"max-width:100%;overflow-x:auto;margin:18px 0\">\n<table style=\"display:table;width:100%;max-width:100%;min-width:0;table-layout:fixed;border-collapse:collapse;font-size:.92em;line-height:1.6\">\n<thead>\n<tr>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#faf0f7;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u540c\u4e00\u4efd\u5019\u9009 BED<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#faf0f7;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u5185\u90e8\u5019\u9009\u6240\u5728\u4e3b\u5e8f\u5217<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#faf0f7;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">\u4e3b\u6587\u4ef6\u603b\u957f<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#faf0f7;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">\u8f6c\u51fa\u78b1\u57fa\u6570<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\"><code style=\"white-space:normal;overflow-wrap:anywhere;word-break:break-word;font-size:.88em\">-e<\/code>\uff0c\u53ea\u5904\u7406\u4e24\u7aef<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u4fdd\u7559\u6574\u6761 50000<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">110000<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">5000<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u9ed8\u8ba4<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">20000 + 23 \u4e2a N + 24000<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">104023<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">11000<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\"><code style=\"white-space:normal;overflow-wrap:anywhere;word-break:break-word;font-size:.88em\">-s<\/code>\uff0c\u5206\u5f00\u8f93\u51fa<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">20000 \u4e0e 24000 \u4e24\u6761\u8bb0\u5f55<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">104000<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">11000<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<figure class=\"guide-figure\" style=\"margin:24px 0 28px\"><img loading=\"lazy\" decoding=\"async\" src=\"\/wordpress\/wp-content\/uploads\/2026\/11\/purge-dups-internal-terminal-get-seqs-23-n-gap-guide.png\" alt=\"\u6e05\u7406 6000 bp \u540e\u591a\u4e86 23 \u4e2a N\u3002\u540c\u4e00\u4efd\u5185\u90e8\u548c\u7aef\u90e8\u5019\u9009\u4ea4\u7ed9 get_seqs\uff0c\u5206\u522b\u6838\u5bf9\u8f6c\u51fa\u5e8f\u5217\u548c\u4fdd\u7559\u7684\u4fa7\u7ffc\u3002\u53ea\u5904\u7406\u4e24\u7aef\uff0c\u5185\u90e8 50000 bp \u5168\u4fdd\u7559\uff0c\u7aef\u90e8 5000 bp \u8fdb\u5165\u5355\u500d\u578b\u7247\u6bb5\u6587\u4ef6\u3002\u9ed8\u8ba4\u5904\u7406\uff0c20000 + 23 N + 24000\uff0c\u5185\u90e8\u5df2\u77e5\u78b1\u57fa\u8f6c\u51fa\uff0c\u4fdd\u7559\u7684\u4e24\u4fa7\u7528\u5360\u4f4d\u8fde\u63a5\u3002\u5206\u5f00\u8f93\u51fa\uff0c20000\u300124000 \u4e24\u6bb5\uff0c\u5206\u5f00\u540e\u4e3b\u6587\u4ef6\u6ca1\u6709\u65b0 N\uff0c\u8f6c\u51fa\u7684\u5019\u9009\u4e0e\u9ed8\u8ba4\u76f8\u540c\u3002\" width=\"1200\" height=\"840\" style=\"display:block;width:100%;height:auto;border-radius:10px\"><figcaption style=\"font-size:14px;line-height:1.6;margin:9px 2px 0;color:#475569\">\u540c\u4e00\u4efd\u5185\u90e8\u548c\u7aef\u90e8\u5019\u9009\u4ea4\u7ed9 get_seqs\uff0c\u5206\u522b\u6838\u5bf9\u8f6c\u51fa\u5e8f\u5217\u548c\u4fdd\u7559\u7684\u4fa7\u7ffc\u3002<\/figcaption><\/figure>\n<p>\u5728\u6279\u91cf\u7edf\u8ba1\u65f6\uff0c\u4e0d\u5fc5\u5148\u628a\u6240\u6709\u5e8f\u5217\u5934\u6539\u6210\u7edf\u4e00\u5f62\u5f0f\u3002\u5bf9\u4e24\u5957\u7ed3\u679c\u5206\u522b\u5efa\u7acb\u6309\u8f93\u5165\u7247\u6bb5\u540d\u7d22\u5f15\u7684\u8868\uff0c\u5c06\u8349\u56fe\u4e2d\u7684\u5b9e\u9645\u78b1\u57fa\u3001\u8f6c\u51fa\u90e8\u5206\u7684\u5b9e\u9645\u78b1\u57fa\u3001\u8f93\u51fa\u4e2d\u65b0\u52a0\u5165\u7684\u672a\u77e5\u5b57\u7b26\u5206\u522b\u5217\u51fa\u3002\u5f53\u539f\u59cb\u8349\u56fe\u4e5f\u542b\u6709\u7f3a\u53e3\u65f6\uff0c\u8fd8\u5e94\u5355\u5217\u539f\u6709\u672a\u77e5\u5b57\u7b26\uff0c\u907f\u514d\u5c06\u5b83\u4eec\u8bef\u7b97\u6210\u8fd9\u6b21\u65b0\u6dfb\u7684\u5360\u4f4d\u3002\u628a\u6bcf\u6761\u8bb0\u5f55\u5404\u81ea\u7ed3\u6e05\uff0c\u518d\u505a\u6837\u672c\u6c47\u603b\uff0c\u53ef\u4ee5\u9632\u6b62\u4e00\u6761\u591a\u7b97\u3001\u53e6\u4e00\u6761\u5c11\u7b97\u5728\u603b\u6570\u4e0a\u521a\u597d\u62b5\u6d88\u3002<\/p>\n<p>\u7b2c\u4e09\u6761\u65e0\u5019\u9009\u5bf9\u7167\u4e5f\u9700\u8981\u505a\u5b8c\u6574\u6bd4\u5bf9\u3002\u5b83\u5728\u4e0d\u540c\u6a21\u5f0f\u4e0b\u5373\u4f7f\u88ab\u52a0\u4e0a\u540d\u79f0\u540e\u7f00\uff0c\u5b9e\u9645\u78b1\u57fa\u4ecd\u5e94\u9010\u5b57\u4e00\u81f4\u3002\u82e5\u8fd9\u4e00\u6b65\u4e0d\u6210\u7acb\uff0c\u5148\u6838\u5bf9 BED \u7684\u67d3\u8272\u4f53\u547d\u540d\u4e0e FASTA \u7248\u672c\uff0c\u6392\u9664\u628a\u4e0a\u4e00\u8f6e\u7ec4\u88c5\u7684\u5750\u6807\u62ff\u5230\u8fd9\u4e00\u8f6e\u5904\u7406\u7684\u53ef\u80fd\uff0c\u518d\u53bb\u89e3\u91ca\u4fdd\u7559\u6216\u8f6c\u51fa\u7684\u751f\u7269\u5b66\u610f\u4e49\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u5728 BED\u3001\u4e3b\u5e8f\u5217\u548c\u8f6c\u51fa\u6587\u4ef6\u4e4b\u95f4\u505a\u5e73\u8861\u8868<\/h2>\n<pre style=\"max-width:100%;box-sizing:border-box;overflow-x:auto;margin:18px 0;padding:14px 16px;background:#f3f6fa;color:#1a293c;border:1px solid #d8e1ea;border-radius:8px;line-height:1.55;white-space:pre;overflow-wrap:normal;word-break:normal\"><code class=\"language-bash\">get_seqs -e -p ends calls.bed primary.fa\r\nget_seqs -p joined calls.bed primary.fa\r\nget_seqs -s -p split calls.bed primary.fa\r\nsamtools faidx joined.purged.fa\r\nsamtools faidx joined.hap.fa\r\n<\/code><\/pre>\n<p>\u5206\u5f00\u8f93\u51fa\u7684\u8fd0\u884c\u628a\u5185\u90e8\u5019\u9009\u4e24\u4fa7\u53d8\u6210\u4e24\u6761\u72ec\u7acb\u7684\u4e3b\u8bb0\u5f55\uff0c\u4e24\u6bb5\u957f\u5ea6\u51c6\u786e\u76f8\u52a0\u4e3a\u56db\u4e07\u56db\u5343\uff0c\u91cc\u9762\u6ca1\u6709\u65b0 N\u3002\u8f6c\u51fa\u6587\u4ef6\u91cc\u4ecd\u662f\u540c\u4e00\u5bf9\u5019\u9009\uff0c\u5206\u522b\u4e3a\u516d\u5343\u548c\u4e94\u5343\uff0c\u9010\u78b1\u57fa\u4e0e\u539f BED \u5b8c\u5168\u4e00\u81f4\u3002\u56e0\u6b64\u9ed8\u8ba4\u548c\u5206\u5f00\u4e24\u79cd\u65b9\u5f0f\u5bf9\u88ab\u5220\u5df2\u77e5\u5e8f\u5217\u7684\u5224\u65ad\u76f8\u540c\uff0c\u5bf9\u4e0b\u6e38\u600e\u6837\u8868\u8fbe\u4e24\u4fa7\u8fde\u63a5\u7684\u5904\u7406\u4e0d\u540c\u3002\u82e5\u4e0b\u6e38\u7b97\u6cd5\u628a\u4e00\u6761 FASTA \u8bb0\u5f55\u5f53\u4f5c\u4e0d\u53ef\u518d\u5206\u5272\u7684\u8fde\u7eed\u5355\u4f4d\uff0c\u5219\u5e94\u4f7f\u7528\u5b9e\u9645\u8f93\u51fa\u7684\u7247\u6bb5\u53ca\u7f3a\u53e3\u5b9a\u4e49\u6765\u8bf4\u660e\u65ad\u70b9\u5982\u4f55\u8868\u793a\u3002\u76f2\u76ee\u53bb\u6389\u6240\u6709 N \u540e\u518d\u62fc\u63a5\u5e8f\u5217\uff0c\u4f1a\u5728\u6ca1\u6709\u989d\u5916\u8bc1\u636e\u7684\u60c5\u51b5\u4e0b\u5236\u9020\u4e00\u4e2a\u76f4\u63a5\u8fde\u63a5\u3002<\/p>\n<p>\u8fc1\u79fb\u57fa\u56e0\u5750\u6807\u65f6\u53ef\u6311\u4e00\u4e2a\u9760\u8fd1\u8fb9\u754c\u7684\u5b57\u6bcd\u9a8c\u7b97\u3002\u539f\u7b2c\u4e00\u6761\u4e0a\u4e00\u57fa\u4e8c\u4e07\u516d\u5343\u96f6\u4e00\uff0c\u662f\u5019\u9009\u4e4b\u540e\u7b2c\u4e00\u4e2a\u4fdd\u7559\u78b1\u57fa\u3002\u9ed8\u8ba4\u7ed3\u679c\u4e2d\u5b83\u5e94\u6392\u5728\u4e24\u4e07\u4e2a\u5de6\u4fa7\u78b1\u57fa\u548c\u4e8c\u5341\u4e09\u4e2a N \u4e4b\u540e\uff0c\u5373\u7b2c\u4e00\u6761\u7684\u65b0\u4e8c\u4e07\u96f6\u4e8c\u5341\u56db\u4f4d\u3002\u5206\u5f00\u8f93\u51fa\u65f6\u5b83\u5374\u662f\u53f3\u7247\u6bb5\u7684\u7b2c\u4e00\u4f4d\u3002\u82e5\u4f7f\u7528\u65e7\u7248\u6ce8\u91ca\u6309\u539f\u5750\u6807\u67e5\u8be2\u65b0\u7248 FASTA\uff0c\u5373\u4f7f\u540c\u540d\u57fa\u56e0\u4ecd\u5728\uff0c\u4e5f\u4f1a\u5728\u9519\u8bef\u4f4d\u7f6e\u8bfb\u53d6\u5e8f\u5217\u3002\u8fd9\u6b21\u4e0d\u540c\u6a21\u5f0f\u8fde\u90e8\u4ef6\u540d\u79f0\u7684\u540e\u7f00\u90fd\u53ef\u80fd\u6539\u53d8\uff0c\u56e0\u6b64\u8f6c\u6362\u8868\u5e94\u4fdd\u5b58\u7248\u53f7\u3001\u539f\u540d\u3001\u539f\u59cb\u533a\u95f4\u3001\u65b0\u540d\u3001\u5728\u65b0\u5e8f\u5217\u4e2d\u7684\u533a\u95f4\u4ee5\u53ca\u65b9\u5411\u3002<\/p>\n<p>\u5b9e\u9645\u51b3\u5b9a\u67d0\u4e2a\u5185\u90e8\u5019\u9009\u662f\u5426\u5e94\u8be5\u6e05\u7406\u65f6\uff0c\u56de\u5230\u652f\u6301\u5b83\u7684\u751f\u7269\u5b66\u6570\u636e\u3002\u5355\u500d\u578b\u91cd\u590d\u8868\u793a\u3001\u8fd1\u671f\u57fa\u56e0\u5bb6\u65cf\u6269\u589e\u3001\u591a\u500d\u4f53\u7684\u540c\u6e90\u533a\u57df\u90fd\u53ef\u80fd\u4ea7\u751f\u76f8\u8fd1\u6bd4\u5bf9\u3002\u68c0\u67e5\u539f\u59cb\u8bfb\u6bb5\u5728\u533a\u57df\u4e24\u4fa7\u7684\u8986\u76d6\u4e0e\u8de8\u8d8a\u652f\u6301\uff0c\u4e5f\u53ef\u4ee5\u7ed3\u5408\u4eb2\u672c\u6807\u8bb0\u4e0e\u62f7\u8d1d\u6570\u8bc1\u636e\u3002\u6279\u91cf\u64cd\u4f5c\u524d\u5148\u53d6\u542b\u7aef\u90e8\u3001\u5185\u90e8\u3001\u65e0\u5019\u9009\u7684\u4e09\u6761\u505a\u590d\u67e5\uff0c\u786e\u8ba4\u5e8f\u5217\u4fdd\u7559\u65b9\u6cd5\u7b26\u5408\u9879\u76ee\u60f3\u4ea4\u4ed8\u7684\u5750\u6807\u7cfb\u7edf\uff0c\u518d\u628a\u540c\u4e00\u7ec4\u89c4\u5219\u7528\u4e8e\u5168\u91cf\u7ec4\u88c5\u3002<\/p>\n<p 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