{"id":71354,"date":"2026-11-15T00:35:19","date_gmt":"2026-11-14T16:35:19","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=71354"},"modified":"2026-11-15T01:14:42","modified_gmt":"2026-11-14T17:14:42","slug":"quast-n50-nga50-translocations-equal-coverage","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/quast-n50-nga50-translocations-equal-coverage\/","title":{"rendered":"QUAST \u5b9e\u8dd1\uff5c\u4e24\u4e2a\u7ec4\u88c5\u7684 N50 \u548c\u8986\u76d6\u7387\u5b8c\u5168\u76f8\u540c\uff0c\u4e3a\u4ec0\u4e48\u4e00\u4e2a\u6709\u4e24\u5904\u9519\u62fc\uff1f"},"content":{"rendered":"
\n

\u53ea\u62ff\u5230\u4e00\u5f20\u7ec4\u88c5\u6307\u6807\u8868\u65f6\uff0c\u4eba\u5f88\u5bb9\u6613\u5148\u6311 N50 \u6700\u5927\u3001\u8986\u76d6\u6700\u5168\u7684\u4e00\u4efd\u3002\u53ef\u662f\u628a\u4e24\u6761\u67d3\u8272\u4f53\u5404\u526a\u5f00\u518d\u4e92\u6362\u4e00\u6bb5\uff0c\u53ea\u8981\u526a\u6cd5\u5bf9\u5f97\u5de7\uff0c\u7ec4\u88c5\u603b\u957f\u3001\u5e8f\u5217\u6761\u6570\u3001N50\u3001\u53c2\u8003\u8986\u76d6\u7387\u90fd\u53ef\u4ee5\u4e00\u6a21\u4e00\u6837\u3002\u7a76\u7adf\u54ea\u9879\u6307\u6807\u80fd\u8bc6\u522b\u8fd9\u79cd\u53d8\u5316\uff1f\u6211\u4eec\u5b89\u88c5 QUAST\uff0c\u5728\u540c\u4e00\u4efd\u6709\u4e24\u6761\u67d3\u8272\u4f53\u7684\u53c2\u8003\u4e0a\u8bc4\u4f30\u4e09\u5957\u5df2\u77e5\u7b54\u6848\u7684\u7ec4\u88c5\u3002\u7b2c\u4e00\u5957\u5168\u5bf9\uff0c\u7b2c\u4e8c\u5957\u4ea4\u6362\u4e24\u4e2a\u8de8\u67d3\u8272\u4f53\u63a5\u5934\uff0c\u7b2c\u4e09\u5957\u5355\u7eaf\u6253\u65ad\u4e00\u6761\u5e8f\u5217\u3002\u7531\u6b64\u53ef\u4ee5\u76f4\u63a5\u770b\u5230\u8fde\u5f97\u957f\u3001\u8fde\u5f97\u5bf9\u3001\u53c2\u8003\u6bcf\u4e00\u4f4d\u6709\u6ca1\u6709\u88ab\u8986\u76d6\u8fd9\u4e09\u79cd\u8bc4\u4ef7\u7684\u533a\u522b\u3002QUAST 5.3.0 \u624b\u518c<\/a><\/p>\n

\u5148\u628a\u6b63\u786e\u3001\u9519\u8bef\u63a5\u5408\u548c\u5355\u7eaf\u65ad\u5f00\u5206\u5f00\u8bbe\u8ba1<\/h2>\n

\u751f\u6210\u4e24\u6761\u968f\u673a\u53c2\u8003\u5e8f\u5217\uff0c\u7532\u5341\u4e8c\u4e07\u78b1\u57fa\uff0c\u4e59\u4e5d\u4e07\u78b1\u57fa\u3002\u7b2c\u4e00\u5957\u7ec4\u88c5\u662f\u5b83\u4eec\u7684\u51c6\u786e\u62f7\u8d1d\uff0c\u4f5c\u4e3a\u9633\u6027\u5bf9\u7167\u3002\u7b2c\u4e8c\u5957\u5c06\u7532\u5206\u6210\u4e24\u4e2a\u516d\u4e07\u78b1\u57fa\u7684\u7247\u6bb5\uff0c\u4e59\u5206\u6210\u4e09\u4e07\u4e0e\u516d\u4e07\u3002\u628a\u7532\u524d\u534a\u6bb5\u63a5\u5230\u4e59\u540e\u6bb5\u5f62\u6210\u4e00\u6761\u5341\u4e8c\u4e07\uff0c\u518d\u628a\u4e59\u524d\u6bb5\u63a5\u5230\u7532\u540e\u534a\u6bb5\u5f62\u6210\u53e6\u4e00\u6761\u4e5d\u4e07\u3002\u6bcf\u4e00\u6bb5\u90fd\u6765\u81ea\u539f\u59cb\u53c2\u8003\uff0c\u78b1\u57fa\u6ca1\u6709\u66ff\u6362\uff0c\u4e5f\u6ca1\u6709\u91cd\u590d\u6216\u9057\u6f0f\uff0c\u4f46\u4e24\u6761\u5e8f\u5217\u5404\u6709\u4e00\u4e2a\u8de8\u67d3\u8272\u4f53\u7684\u4eba\u5de5\u63a5\u5934\u3002\u7b2c\u4e09\u5957\u53ea\u5728\u7532\u7684\u4e2d\u95f4\u65ad\u5f00\uff0c\u5206\u522b\u4fdd\u7559\u4e24\u4e2a\u516d\u4e07\u53ca\u5b8c\u6574\u7684\u4e5d\u4e07\u4e59\uff0c\u4e0d\u589e\u52a0\u4efb\u4f55\u8fde\u63a5\u3002<\/p>\n

\u5c06\u4e09\u5957 FASTA \u653e\u8fdb\u540c\u4e00\u6b21 QUAST 5.3.0 \u8fd0\u884c\uff0c\u4f7f\u7528\u540c\u4e00\u4efd\u5df2\u77e5\u53c2\u8003\u3001\u76f8\u540c\u6700\u77ed\u7247\u6bb5\u89c4\u5219\u548c\u6bd4\u5bf9\u53c2\u6570\u3002\u8fd9\u6837\u7684\u8bbe\u7f6e\u53ef\u4ee5\u907f\u514d\u4e00\u7ec4\u56e0\u4f7f\u7528\u4e0d\u540c\u53c2\u8003\u7248\u672c\u6216\u4e0d\u540c\u957f\u5ea6\u8fc7\u6ee4\u800c\u663e\u5f97\u66f4\u597d\u3002\u4eba\u5de5\u5e8f\u5217\u8bbe\u8ba1\u6210\u65e0\u9884\u8bbe\u957f\u91cd\u590d\uff0c\u662f\u4e3a\u4e86\u8ba9\u4e24\u5904\u8de8\u67d3\u8272\u4f53\u8fde\u63a5\u80fd\u88ab\u6e05\u695a\u6307\u8ba4\u3002\u5728\u91cd\u590d\u591a\u3001\u4eb2\u7f18\u8f83\u8fdc\u6216\u5b58\u5728\u771f\u5b9e\u91cd\u6392\u7684\u6837\u672c\u4e2d\uff0c\u76f8\u540c\u6307\u6807\u7684\u751f\u7269\u5b66\u89e3\u91ca\u8fd8\u5f97\u52a0\u4e0a\u53c2\u8003\u8c31\u7cfb\u548c\u8bfb\u6bb5\u8bc1\u636e\u3002\u8fd9\u91cc\u7684\u9519\u8bef\u4e4b\u6240\u4ee5\u53ef\u4ee5\u786e\u5b9a\uff0c\u662f\u56e0\u4e3a\u6211\u4eec\u81ea\u5df1\u77e5\u9053\u6bcf\u4e2a\u78b1\u57fa\u7247\u6bb5\u539f\u6765\u7684\u8fde\u63a5\u4f4d\u7f6e\u3002<\/p>\n

\u4e3a\u4ec0\u4e48 N50 \u548c\u8986\u76d6\u7387\u4e00\u8d77\u88ab\u201c\u9a97\u8fc7\u201d<\/h2>\n

N50 \u7684\u8ba1\u7b97\u53ea\u9700\u8981\u5404\u5e8f\u5217\u957f\u5ea6\u3002\u628a\u7247\u6bb5\u4ece\u957f\u5230\u77ed\u6392\u5217\uff0c\u7d2f\u8ba1\u5230\u7ec4\u88c5\u957f\u5ea6\u4e00\u534a\u65f6\uff0c\u5f53\u524d\u7247\u6bb5\u7684\u957f\u5ea6\u5c31\u662f N50\u3002\u7b2c\u4e00\u3001\u4e8c\u5957\u7684\u957f\u5ea6\u5217\u8868\u90fd\u662f\u5341\u4e8c\u4e07\u548c\u4e5d\u4e07\uff0c\u603b\u957f\u4e8c\u5341\u4e00\u4e07\uff0c\u4e00\u534a\u662f\u5341\u4e07\u4e94\u5343\u3002\u6392\u7b2c\u4e00\u7684\u5341\u4e8c\u4e07\u5df2\u7ecf\u8d8a\u8fc7\u95e8\u69db\uff0c\u6240\u4ee5\u4e24\u5957\u90fd\u662f\u5341\u4e8c\u4e07\u3002\u4e2d\u95f4\u5230\u5e95\u63a5\u7684\u662f\u540c\u4e00\u6761\u67d3\u8272\u4f53\u8fd8\u662f\u53e6\u4e00\u6761\u67d3\u8272\u4f53\uff0c\u957f\u5ea6\u5217\u8868\u91cc\u6ca1\u6709\u8fd9\u9879\u4fe1\u606f\u3002\u7b2c\u4e09\u5957\u7684\u5217\u8868\u662f\u4e5d\u4e07\u3001\u516d\u4e07\u3001\u516d\u4e07\uff0c\u7b2c\u4e00\u6761\u5c1a\u672a\u8fc7\u7ebf\uff0c\u52a0\u5230\u7b2c\u4e8c\u6761\u65f6\u8fc7\u7ebf\uff0cN50 \u56e0\u800c\u964d\u4e3a\u516d\u4e07\u3002<\/p>\n

\u53c2\u8003\u8986\u76d6\u7387\u95ee\u7684\u662f\u53c2\u8003\u4e0a\u6709\u591a\u5c11\u4f4d\u7f6e\u81f3\u5c11\u843d\u5165\u7ec4\u88c5\u5bf9\u9f50\u4e2d\u3002\u4e09\u5957\u90fd\u5b8c\u6574\u4f7f\u7528\u4e86\u539f\u59cb\u53c2\u8003\u7684\u78b1\u57fa\uff0c\u56e0\u6b64\u5b9e\u9645\u62a5\u544a\u7684\u53c2\u8003\u8986\u76d6\u7387\u5168\u90e8\u4e3a\u767e\u5206\u4e4b\u767e\uff0c\u91cd\u590d\u6bd4\u4e5f\u90fd\u4e3a\u4e00\u70b9\u96f6\u96f6\u96f6\u3002\u7b2c\u4e8c\u5957\u4e71\u63a5\u7684\u4e24\u4e2a\u7247\u6bb5\u5206\u522b\u4ecd\u80fd\u6bd4\u56de\u5404\u81ea\u6765\u6e90\uff0c\u5b83\u4eec\u7684\u5e76\u96c6\u628a\u53c2\u8003\u8986\u76d6\u5f97\u5f88\u5b8c\u6574\u3002\u5728\u4e00\u6b21\u6279\u91cf\u7ec4\u88c5\u91cc\u9047\u5230\u767e\u5206\u4e4b\u767e\u8986\u76d6\u540c\u65f6\u8fd8\u6709\u91cd\u5927\u9519\u62fc\u7684\u63d0\u793a\uff0c\u8fd9\u4e24\u4e2a\u6570\u5b57\u662f\u53ef\u4ee5\u5171\u5b58\u7684\u3002\u8981\u9a8c\u8bc1\u9519\u8bef\u5230\u5e95\u5728\u54ea\uff0c\u9700\u8981\u67e5\u770b\u6bcf\u6761\u67e5\u8be2\u7684\u8fde\u7eed\u6bd4\u5bf9\u5757\u53ca\u5176\u76f8\u90bb\u5173\u7cfb\uff0c\u4e0d\u80fd\u671f\u5f85\u8986\u76d6\u7387\u76f4\u63a5\u8bf4\u51fa\u63a5\u5934\u662f\u5426\u53ef\u9760\u3002<\/p>\n

\n\n\n\n\n\n\n\n
\u7ec4\u88c5<\/th>\n\u5e8f\u5217\u6570<\/th>\nN50<\/th>\nQUAST \u8bc6\u522b\u7684\u91cd\u5927\u9519\u62fc<\/th>\nNGA50<\/th>\n\u53c2\u8003\u8986\u76d6<\/th>\n<\/tr>\n<\/thead>\n
\u539f\u6837\u4e24\u6761\u67d3\u8272\u4f53<\/td>\n2<\/td>\n120000<\/td>\n0<\/td>\n120000<\/td>\n100%<\/td>\n<\/tr>\n
\u4ea4\u6362\u4e24\u5904\u8de8\u67d3\u8272\u4f53\u63a5\u5934<\/td>\n2<\/td>\n120000<\/td>\n2<\/td>\n60000<\/td>\n100%<\/td>\n<\/tr>\n
\u4ec5\u628a\u7532\u5206\u6210\u4e24\u6bb5<\/td>\n3<\/td>\n60000<\/td>\n0<\/td>\n60000<\/td>\n100%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"N50
\u4e09\u5957\u7b49\u957f\u7684\u4eba\u5de5\u7ec4\u88c5\uff0c\u4e00\u5957\u6b63\u786e\uff0c\u4e00\u5957\u9519\u63a5\u4e24\u5904\uff0c\u4e00\u5957\u4fdd\u5b88\u5730\u65ad\u5f00\u3002<\/figcaption><\/figure>\n

\u82e5\u6bd4\u8f83\u591a\u4efd\u6765\u81ea\u4e0d\u540c\u4eb2\u7f18\u80cc\u666f\u7684\u6837\u672c\uff0c\u5148\u7ed9\u53c2\u8003\u5e8f\u5217\u5efa\u7acb\u540c\u4e00\u7248\u6e05\u5355\uff0c\u6ce8\u660e\u662f\u5426\u5305\u542b\u66ff\u4ee3\u5e8f\u5217\u3001\u7ec6\u80de\u5668\u548c\u672a\u5b9a\u4f4d\u7247\u6bb5\u3002\u53ea\u8981\u6539\u53d8\u53c2\u8003\u603b\u957f\u6216\u7eb3\u5165\u7684\u53c2\u8003\u96c6\u5408\uff0c\u8fbe\u5230\u4e00\u534a\u6240\u9700\u7684\u7d2f\u8ba1\u957f\u5ea6\u5c31\u53ef\u80fd\u6539\u53d8\u3002\u5bf9\u77ed\u7247\u6bb5\u8f83\u591a\u7684\u7ec4\u88c5\uff0c\u8fd8\u8981\u8bb0\u5f55\u6700\u77ed\u7eb3\u5165\u9608\u503c\u3002\u8fd9\u6837\u8bfb\u8005\u624d\u80fd\u6309\u62a5\u544a\u91cc\u7684\u539f\u59cb\u957f\u5ea6\u91cd\u65b0\u7b97\u51fa\u6bcf\u4e2a\u6570\uff0c\u5e76\u5224\u65ad\u4e24\u4e2a\u6837\u672c\u7684\u5dee\u5f02\u662f\u5426\u53d1\u751f\u5728\u540c\u4e00\u8bc4\u4f30\u8303\u56f4\u3002<\/p>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u628a NGA50 \u63a5\u56de\u539f\u59cb\u6bd4\u5bf9\u5757<\/h2>\n
quast.py -t 2 --no-plots --no-html --no-icarus --no-gc -r reference.fa -o results intact.fa translocated.fa split.fa\r\nawk -F '\\t' '$1=="N50" || $1=="NGA50" || $1=="# misassemblies" || $1=="Genome fraction (%)"' results\/report.tsv\r\n<\/code><\/pre>\n
QUAST \u8ba1\u7b97\u4e0e\u53c2\u8003\u6bd4\u5bf9\u6709\u5173\u7684\u8fde\u7eed\u6027\u65f6\uff0c\u4f1a\u5728\u76f8\u5e94\u7684\u9519\u62fc\u8fb9\u754c\u62c6\u5206\u6bd4\u5bf9\uff0c\u518d\u6309\u5bf9\u9f50\u5757\u957f\u5ea6\u7edf\u8ba1\u3002NGA50 \u7528\u53c2\u8003\u603b\u957f\u5ea6\u4f5c\u4e3a\u8fbe\u5230\u4e00\u534a\u65f6\u7684\u5206\u6bcd\u3002\u7b2c\u4e00\u5957\u4fdd\u7559\u4e86\u5b8c\u6574\u5341\u4e8c\u4e07\uff0c\u7b2c\u4e8c\u5957\u4e24\u6761\u4eba\u5de5\u8fde\u63a5\u88ab\u8bc6\u522b\u4e3a\u4e24\u5904\u91cd\u5927\u9519\u62fc\uff0c\u62c6\u5f00\u540e NGA50 \u53ea\u5269\u516d\u4e07\u3002\u7b2c\u4e09\u5957\u540c\u6837\u662f\u516d\u4e07\uff0c\u4f46\u5e76\u6ca1\u6709\u9519\u8bef\u8fde\u63a5\u3002\u4e8e\u662f\u5373\u4f7f\u62ff\u5230\u4e86\u4e00\u4e2a\u66f4\u91cd\u89c6\u6b63\u786e\u6027\u7684\u8fde\u7eed\u6307\u6807\uff0c\u4ecd\u8981\u4e0e\u9519\u62fc\u6570\u3001\u672a\u6bd4\u5bf9\u957f\u5ea6\u4e00\u8d77\u770b\uff0c\u624d\u80fd\u533a\u5206\u201c\u9519\u8bef\u63a5\u957f\u518d\u88ab\u62c6\u5f00\u201d\u548c\u201c\u539f\u672c\u5c31\u5f88\u4fdd\u5b88\u5730\u7559\u4e86\u65ad\u70b9\u201d\u3002QUAST \u4f5c\u8005\u9879\u76ee\u4e0e\u53c2\u8003\u8bc4\u4f30\u5de5\u5177<\/a><\/p>\n
\u8fd9\u6b21\u8fd8\u6709\u4e00\u4e2a\u5bb9\u6613\u5ffd\u7565\u7684\u7ec6\u8282\u3002\u7b2c\u4e8c\u5957\u4e2d\u6700\u957f\u7684\u5bf9\u9f50\u5757\u62a5\u544a\u4e3a\u516d\u4e07\u96f6\u4e00\u4e2a\u78b1\u57fa\uff0c\u800c\u4eba\u5de5\u62fc\u63a5\u65f6\u5236\u4f5c\u7684\u957f\u5757\u90fd\u662f\u516d\u4e07\u3002\u5c40\u90e8\u6bd4\u5bf9\u53ef\u4ee5\u5728\u8fb9\u754c\u5ef6\u4f38\uff0c\u6070\u597d\u76f8\u540c\u7684\u76f8\u90bb\u78b1\u57fa\u6216\u6bd4\u5bf9\u8fb9\u754c\u9009\u62e9\u4f1a\u4f7f\u62a5\u544a\u533a\u95f4\u4e0e\u624b\u5de5\u5207\u70b9\u76f8\u5dee\u4e00\u4f4d\u3002\u6ca1\u6709\u9010\u4f4d\u68c0\u67e5\u524d\uff0c\u4e0d\u80fd\u76f4\u63a5\u628a\u8fd9\u4e00\u4e2a\u78b1\u57fa\u89e3\u91ca\u6210\u53d1\u751f\u4e86\u4e00\u6b21\u63d2\u5165\u3002\u82e5\u8981\u62a5\u544a\u771f\u5b9e\u65ad\u70b9\uff0c\u5e94\u627e\u51fa\u67e5\u8be2\u4e2d\u7684\u5de6\u53f3\u951a\u70b9\u3001\u53c2\u8003\u843d\u70b9\u548c\u65b9\u5411\uff0c\u68c0\u67e5\u4e24\u4fa7\u662f\u5426\u6709\u4e00\u5c0f\u6bb5\u53ef\u4f9b\u591a\u79cd\u7b49\u4ef7\u653e\u7f6e\u7684\u5e8f\u5217\uff0c\u5e76\u628a\u53ef\u80fd\u8303\u56f4\u4fdd\u7559\u4e0b\u6765\u3002<\/p>\n
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