{"id":71372,"date":"2026-11-15T00:39:46","date_gmt":"2026-11-14T16:39:46","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=71372"},"modified":"2026-11-15T01:14:39","modified_gmt":"2026-11-14T17:14:39","slug":"merqury-qv-read-coverage-gap-kmer-assembly","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/merqury-qv-read-coverage-gap-kmer-assembly\/","title":{"rendered":"Merqury \u5b9e\u8dd1\uff5c\u540c\u4e00\u4efd\u7ec4\u88c5\uff0c\u4e3a\u4ec0\u4e48\u6362\u4e00\u6279\u8bc4\u4f30\u8bfb\u6bb5\uff0cQV \u4ece 34 \u964d\u5230 23\uff1f"},"content":{"rendered":"
\n

\u5047\u8bbe\u4f60\u62ff\u5230\u540c\u4e00\u4e2a\u6837\u672c\u7684\u4e24\u4efd\u8d28\u63a7\u62a5\u544a\uff0c\u7ec4\u88c5\u6587\u4ef6\u7684\u6821\u9a8c\u548c\u4e00\u6837\uff0c\u4e00\u4efd\u62a5 QV 34\uff0c\u53e6\u4e00\u4efd\u53ea\u6709 23\u3002\u56de\u5934\u68c0\u67e5\u629b\u5149\u7a0b\u5e8f\uff0c\u5f88\u53ef\u80fd\u67e5\u9519\u4e86\u5730\u65b9\u3002Merqury \u7528\u51c6\u786e\u8bfb\u6bb5\u4e3a\u7ec4\u88c5\u4e2d\u7684\u77ed\u5e8f\u5217\u4f5c\u8bc1\u3002\u5b83\u65e2\u4f1a\u5bdf\u89c9\u5199\u9519\u7684\u78b1\u57fa\uff0c\u4e5f\u4f1a\u5bdf\u89c9\u8bfb\u6bb5\u5e93\u6ca1\u80fd\u66ff\u4e00\u6bb5\u6b63\u786e\u5e8f\u5217\u4f5c\u8bc1\u3002\u6211\u4eec\u5728\u4e00\u6761\u5df2\u77e5\u771f\u503c\u7684\u5e8f\u5217\u4e0a\u5206\u522b\u5236\u9020\u8fd9\u4e24\u79cd\u60c5\u51b5\uff0c\u8ffd\u8e2a\u603b\u5206\u3001\u77ed\u5e8f\u5217\u8ba1\u6570\u548c\u9519\u8bef\u533a\u95f4\u5982\u4f55\u4e00\u8d77\u53d8\u5316\u3002Merqury \u65b9\u6cd5\u8bba\u6587<\/a><\/p>\n

\u5148\u95ee\u54ea\u4e2a\u96c6\u5408\u5728\u505a\u5206\u6bcd<\/h2>\n

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\u4e00\u4e2a\u5b64\u7acb\u7684\u78b1\u57fa\u5199\u9519\uff0c\u4f1a\u7834\u574f\u76d6\u4f4f\u5b83\u7684\u4e00\u4e32\u7a97\u53e3\u3002\u53ea\u8981\u79bb\u4e24\u7aef\u8db3\u591f\u8fdc\uff0c\u957f\u5ea6\u4e3a\u4e8c\u5341\u4e00\u7684\u7a97\u53e3\u6700\u591a\u6709\u4e8c\u5341\u4e00\u4e2a\u76d6\u4f4f\u8fd9\u4e2a\u4f4d\u7f6e\u3002\u628a\u4e8c\u5341\u4e00\u4e2a\u5f02\u5e38\u8bcd\u9664\u4ee5\u7ec4\u88c5\u957f\u5ea6\uff0c\u4f1a\u628a\u540c\u4e00\u5904\u6539\u53d8\u7b97\u4e0a\u5f88\u591a\u904d\u3002\u811a\u672c\u5148\u4f30\u8ba1\u4e00\u4e2a\u5b8c\u6574\u7a97\u53e3\u88ab\u652f\u6301\u7684\u6bd4\u4f8b\uff0c\u518d\u6309\u7a97\u53e3\u957f\u5ea6\u6298\u56de\u5230\u5355\u78b1\u57fa\u9519\u8bef\u6982\u7387\uff0c\u6700\u540e\u8f6c\u6210\u5bf9\u6570\u5206\u6570\u3002\u76f8\u90bb\u9519\u8bef\u5171\u4eab\u7a97\u53e3\uff0c\u91cd\u590d\u5e8f\u5217\u53ef\u80fd\u5728\u522b\u5904\u83b7\u5f97\u652f\u6301\uff0c\u771f\u5b9e\u57fa\u56e0\u7ec4\u5f88\u5c11\u6ee1\u8db3\u6700\u7b80\u5355\u7684\u72ec\u7acb\u6a21\u578b\u3002\u628a\u5206\u6570\u8bf4\u660e\u4e3a\u57fa\u4e8e\u8bfb\u6bb5\u652f\u6301\u7684\u4f30\u8ba1\uff0c\u8bfb\u8005\u624d\u80fd\u5224\u65ad\u5b83\u80fd\u56de\u7b54\u591a\u7ec6\u7684\u95ee\u9898\u3002<\/p>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u7ec4\u88c5\u91cc\u59cb\u7ec8\u53ea\u6709\u4e00\u4e2a\u66ff\u6362<\/h2>\n

\u6211\u4eec\u7528\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u751f\u6210\u957f\u4e24\u5343\u516d\u767e\u78b1\u57fa\u7684\u771f\u503c\uff0c\u590d\u5236\u4e00\u4efd\uff0c\u5728\u4ece\u4e00\u5f00\u59cb\u8ba1\u6570\u7684\u7b2c\u4e00\u5343\u4e09\u767e\u96f6\u4e00\u4f4d\u6539\u5199\u4e00\u4e2a\u78b1\u57fa\u3002\u8bc4\u4f30\u8bfb\u6bb5\u59cb\u7ec8\u53d6\u81ea\u539f\u59cb\u771f\u503c\uff0c\u6bcf\u6761\u4e00\u767e\u4e94\u5341\u78b1\u57fa\u3002\u7b2c\u4e00\u7ec4\u4ee5\u4e94\u5341\u78b1\u57fa\u6b65\u957f\u5e73\u94fa\u5230\u672b\u7aef\uff0c\u5171\u4e94\u5341\u6761\u3002\u7b2c\u4e8c\u7ec4\u5206\u522b\u94fa\u5728\u9884\u5148\u9009\u5b9a\u7684\u7f3a\u53e3\u4e24\u4fa7\uff0c\u5171\u56db\u5341\u56db\u6761\uff0c\u4e2d\u95f4\u4e24\u767e\u4e2a\u78b1\u57fa\u6ca1\u6709\u4efb\u4f55\u8bfb\u6bb5\u7ecf\u8fc7\u3002\u4e24\u6b21\u8fd0\u884c\u8bfb\u5165\u540c\u4e00\u4efd\u7ec4\u88c5\uff0c\u5747\u7528 meryl 1.4.2 \u5efa\u5e93\uff0c\u518d\u8c03\u7528 Merqury 1.4.1 \u81ea\u5e26\u811a\u672c\u3002\u8fd9\u4e2a\u8f93\u5165\u523b\u610f\u6d88\u9664\u4e86\u6d4b\u5e8f\u566a\u58f0\uff0c\u8ba9\u7f3a\u5c11\u53d6\u6837\u8fd9\u4ef6\u4e8b\u53ef\u4ee5\u5355\u72ec\u89c2\u5bdf\u3002<\/p>\n

\n\n\n\n\n\n\n
\u8bfb\u6bb5\u6761\u4ef6<\/th>\n\u7ec4\u88c5\u72ec\u6709\u8bcd \/ \u7ec4\u88c5\u8bcd<\/th>\nQV<\/th>\n\u811a\u672c\u8f93\u51fa\u7684\u96f6\u8d77\u70b9\u534a\u5f00\u533a\u95f4<\/th>\n<\/tr>\n<\/thead>\n
\u5b8c\u6574\u94fa\u8fc7\u771f\u503c<\/td>\n21 \/ 2580<\/td>\n34.0993<\/td>\n[1280,1321)<\/td>\n<\/tr>\n
\u4e2d\u95f4\u7f3a\u5c11 200 bp \u8bfb\u6bb5\u8986\u76d6<\/td>\n241 \/ 2580<\/td>\n23.3171<\/td>\n[1280,1321) \u548c [1780,2020)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u8fd9\u4e2a\u7279\u5236\u5e8f\u5217\u7684\u7ec4\u88c5\u7a97\u53e3\u6070\u597d\u5168\u90e8\u4e0d\u540c\uff0c\u56e0\u6b64\u5206\u6bcd\u4e24\u5343\u4e94\u767e\u516b\u5341\u4e5f\u53ef\u4ee5\u4ece\u5e8f\u5217\u957f\u5ea6\u51cf\u53bb\u4e8c\u5341\u76f4\u63a5\u590d\u6838\u3002\u7b2c\u4e00\u7ec4\u53ea\u6709\u8de8\u8fc7\u66ff\u6362\u7684\u4e8c\u5341\u4e00\u4e2a\u8bcd\u5339\u914d\u5931\u8d25\uff0c\u6a21\u578b\u7ed9\u51fa\u7684\u78b1\u57fa\u9519\u8bef\u7387\u7ea6\u4e3a\u4e07\u5206\u4e4b\u4e09\u70b9\u4e5d\uff0c\u548c\u771f\u503c\u4e2d\u7684\u4e00\u4e2a\u9519\u8bef\u9664\u4ee5\u4e24\u5343\u516d\u767e\u76f8\u8fd1\u3002\u7b2c\u4e8c\u7ec4\u591a\u51fa\u4e8c\u767e\u4e8c\u5341\u4e2a\u7ec4\u88c5\u72ec\u6709\u8bcd\uff0c\u539f\u6765\u7684\u66ff\u6362\u4ecd\u5728\u90a3\u91cc\uff0c\u4f46\u65b0\u5f02\u5e38\u6765\u81ea\u62bd\u6837\u7f3a\u53e3\u3002\u4ece\u4e0a\u6e38\u518d\u505a\u4e00\u8f6e\u629b\u5149\uff0c\u4e5f\u65e0\u6cd5\u51ed\u7a7a\u8865\u56de\u8bfb\u6bb5\u5e93\u91cc\u7f3a\u5c11\u7684\u8bc1\u636e\u3002<\/p>\n

\"\u4e00\u4efd\u7ec4\u88c5\uff0c\u4e24\u79cd
\u540c\u4e00\u4e2a\u66ff\u6362\u4fdd\u7559\u4e0d\u52a8\uff0c\u53ea\u5728\u8bc4\u4f30\u8bfb\u6bb5\u4e2d\u7559\u4e00\u5904\u7f3a\u53e3\u3002<\/figcaption><\/figure>\n

\u533a\u95f4\u7684\u8fb9\u754c\u5c24\u5176\u5bb9\u6613\u8bef\u8bfb\u3002\u7b2c\u4e00\u7ec4\u7684\u5f02\u5e38\u533a\u95f4\u957f\u56db\u5341\u4e00\u4e2a\u78b1\u57fa\uff0c\u77e5\u9053\u7b54\u6848\u7684\u6211\u4eec\u5374\u53ea\u6539\u8fc7\u6b63\u4e2d\u4e00\u4e2a\u4f4d\u7f6e\u3002\u811a\u672c\u62a5\u544a\u7684\u662f\u8fde\u7eed\u5f02\u5e38\u8bcd\u8986\u76d6\u8303\u56f4\u7684\u5e76\u96c6\uff0c\u6700\u5de6\u8fb9\u7684\u8bcd\u4ece\u66ff\u6362\u70b9\u4e0a\u6e38\u4e8c\u5341\u4f4d\u5f00\u59cb\uff0c\u6700\u53f3\u8fb9\u7684\u8bcd\u53c8\u5411\u4e0b\u6e38\u5ef6\u4f38\u4e8c\u5341\u4f4d\u3002\u7b2c\u4e8c\u7ec4\u65b0\u589e\u533a\u95f4\u957f\u4e8c\u767e\u56db\u5341\u4e2a\u78b1\u57fa\uff0c\u800c\u4eba\u5de5\u7559\u4e0b\u7684\u7f3a\u53e3\u53ea\u6709\u4e8c\u767e\u4e2a\u3002\u628a\u8fd9\u4efd\u533a\u95f4\u6e05\u5355\u76f4\u63a5\u5f53\u4f5c\u9700\u8981\u526a\u9664\u7684\u5e8f\u5217\uff0c\u4f1a\u5728\u4e24\u8fb9\u4e00\u5e76\u4e22\u6389\u6709\u8bc1\u636e\u7684\u78b1\u57fa\u3002\u5e94\u5148\u56de\u5230\u539f\u59cb\u6bd4\u5bf9\u6216\u6df1\u5ea6\u8f68\u8ff9\uff0c\u67e5\u770b\u5185\u90e8\u4e0e\u8fb9\u7f18\u5206\u522b\u53d1\u751f\u4e86\u4ec0\u4e48\u3002<\/p>\n

\u600e\u6837\u590d\u7b97\u62a5\u544a\u4e0a\u7684\u6570<\/h2>\n
meryl count threads=2 memory=1 k=21 output reads.meryl reads.fa\r\nbash "$MERQURY\/eval\/qv.sh" "$PWD\/reads.meryl" "$PWD\/draft.fa" report\r\ncat report.qv\r\ncat report.draft.qv\r\ncat report_only.bed\r\n<\/code><\/pre>\n
\u6211\u4eec\u53e6\u5916\u5199\u4e86\u4e00\u4e2a\u77ed\u7a0b\u5e8f\uff0c\u5bf9\u6b63\u5411\u8bcd\u4e0e\u53cd\u5411\u4e92\u8865\u8bcd\u53d6\u5b57\u5178\u5e8f\u8f83\u5c0f\u8005\uff0c\u5efa\u7acb\u4e24\u4e2a\u96c6\u5408\uff0c\u76f4\u63a5\u6570\u51fa\u53ea\u5728\u7ec4\u88c5\u91cc\u51fa\u73b0\u7684\u5143\u7d20\u3002\u4e24\u79cd\u8f93\u5165\u7684\u6570\u91cf\u90fd\u548c\u811a\u672c\u76f8\u7b49\u3002\u518d\u7528\u7ec4\u88c5\u72ec\u6709\u8bcd\u6570\u9664\u4ee5\u603b\u8bcd\u6570\uff0c\u53d6\u5176\u8865\u6570\u7684\u4e8c\u5341\u4e00\u6b21\u65b9\u6839\uff0c\u7528\u4e00\u51cf\u53bb\u8fd9\u4e2a\u6839\u5c31\u5f97\u5230\u4f30\u8ba1\u9519\u8bef\u7387\u3002\u4ee5\u5341\u4e3a\u5e95\u53d6\u5bf9\u6570\u540e\u4e58\u8d1f\u5341\uff0c\u4e24\u7ec4\u6570\u5b57\u90fd\u80fd\u590d\u7b97\u5230\u5c0f\u6570\u70b9\u540e\u56db\u4f4d\u3002\u771f\u5b9e\u9879\u76ee\u5305\u542b\u91cd\u590d\u8bcd\u65f6\uff0c\u5e94\u7167\u6240\u7528\u7248\u672c\u811a\u672c\u6838\u5bf9\u8ba1\u6570\u5b9a\u4e49\uff0c\u4e0d\u80fd\u628a\u8fd9\u6761\u968f\u673a\u5e8f\u5217\u4e0a\u78b0\u5de7\u76f8\u7b49\u7684\u201c\u7a97\u53e3\u6570\u201d\u548c\u201c\u4e0d\u540c\u8bcd\u6570\u201d\u76f4\u63a5\u4e92\u6362\u3002meryl \u8ba1\u6570\u5de5\u5177<\/a><\/p>\n
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