{"id":71409,"date":"2026-11-15T00:49:54","date_gmt":"2026-11-14T16:49:54","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=71409"},"modified":"2026-11-15T01:14:45","modified_gmt":"2026-11-14T17:14:45","slug":"busco-proteome-completeness-duplicated-markers-ecoli","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/busco-proteome-completeness-duplicated-markers-ecoli\/","title":{"rendered":"BUSCO \u5b9e\u8dd1\uff5c\u86cb\u767d\u96c6\u5b8c\u6574\u5ea6\u540c\u4e3a 100%\uff0c\u5982\u4f55\u53d1\u73b0\u591a\u4ea4\u4e86\u4e09\u6761\u86cb\u767d\uff1f"},"content":{"rendered":"
\n

\u7ed9\u4e24\u7248\u6ce8\u91ca\u505a\u9a8c\u6536\u65f6\uff0c\u5e38\u89c1\u505a\u6cd5\u662f\u5148\u770b BUSCO \u5b8c\u6574\u5ea6\u3002\u5047\u5982\u4e24\u7248\u90fd\u662f\u767e\u5206\u4e4b\u767e\uff0c\u5176\u4e2d\u4e00\u7248\u5374\u6bd4\u53e6\u4e00\u7248\u591a\u51fa\u4e09\u6761\u86cb\u767d\uff0c\u4f60\u80fd\u4ece\u62a5\u544a\u91cc\u627e\u5230\u662f\u54ea\u4e09\u6761\u5417\uff1f\u6211\u4eec\u4e0b\u8f7d\u4e86\u5927\u80a0\u6746\u83cc K-12 MG1655 \u7684\u516c\u5f00\u86cb\u767d\u96c6\uff0c\u5148\u8dd1\u539f\u6837\u6570\u636e\uff0c\u518d\u4eba\u4e3a\u590d\u5236\u4e09\u6761\uff0c\u6700\u540e\u79fb\u9664\u53e6\u4e00\u6761\u3002\u4e09\u6b21\u4f7f\u7528\u540c\u4e00\u5957\u7ec6\u83cc\u6807\u8bb0\uff0c\u5dee\u522b\u65e2\u80fd\u5728\u6c47\u603b\u884c\u4e2d\u770b\u5230\uff0c\u4e5f\u80fd\u6cbf\u7740\u6807\u8bb0\u7f16\u53f7\u4e00\u76f4\u8ffd\u5230\u5177\u4f53\u86cb\u767d\u3002NCBI \u53c2\u8003\u7ec4\u88c5\u53ca\u86cb\u767d\u6570\u636e<\/a><\/p>\n

\u770b\u89c1\u5b8c\u6574\u5ea6\u524d\uff0c\u5148\u786e\u8ba4\u5728\u6570\u4ec0\u4e48<\/h2>\n

BUSCO \u4e3a\u6240\u9009\u8c31\u7cfb\u51c6\u5907\u4e86\u4e00\u7ec4\u9884\u671f\u5728\u5927\u591a\u6570\u6210\u5458\u4e2d\u51fa\u73b0\u3001\u901a\u5e38\u4e3a\u5355\u62f7\u8d1d\u7684\u76f4\u7cfb\u540c\u6e90\u6807\u8bb0\u3002\u86cb\u767d\u6a21\u5f0f\u628a\u8f93\u5165\u86cb\u767d\u4e0e\u6807\u8bb0\u6a21\u578b\u76f8\u6bd4\u8f83\uff0c\u6839\u636e\u5f97\u5206\u3001\u5339\u914d\u957f\u5ea6\u7b49\u6761\u4ef6\u51b3\u5b9a\u80fd\u5426\u627e\u5230\u8db3\u591f\u5b8c\u6574\u7684\u4ee3\u8868\u3002\u8868\u91cc\u6bcf\u4e2a\u6807\u8bb0\u53ea\u6709\u4e00\u4e2a\u5206\u7c7b\u3002\u5982\u679c\u4e00\u4e2a\u6807\u8bb0\u627e\u5230\u4e24\u4e2a\u8db3\u591f\u5b8c\u6574\u7684\u4ee3\u8868\uff0c\u4f1a\u5f52\u5230\u5b8c\u6574\u4e14\u91cd\u590d\u3002\u628a\u5b83\u548c\u5b8c\u6574\u5355\u62f7\u8d1d\u76f8\u52a0\uff0c\u4ecd\u7136\u53ea\u7ed9\u603b\u5b8c\u6574\u6807\u8bb0\u6570\u8d21\u732e\u4e00\u9879\u3002\u770b\u89c1\u5b8c\u6574\u5ea6\u5f88\u9ad8\uff0c\u53ea\u80fd\u8bf4\u8fd9\u7ec4\u6807\u8bb0\u5927\u591a\u627e\u5230\u4e86\uff0c\u65e0\u6cd5\u7531\u6b64\u6570\u51fa\u6574\u4efd\u6ce8\u91ca\u91cc\u6709\u591a\u5c11\u6761\u86cb\u767d\u91cd\u590d\u3002BUSCO \u7528\u6237\u6307\u5357<\/a><\/p>\n

\u8fd9\u4e00\u70b9\u51b3\u5b9a\u4e86\u5bf9\u7167\u600e\u4e48\u8bbe\u8ba1\u3002\u5355\u7eaf\u5411\u6587\u4ef6\u5c3e\u90e8\u52a0\u51e0\u6761\u4efb\u610f\u5e8f\u5217\uff0c\u6570\u5b57\u53ef\u80fd\u5b8c\u5168\u4e0d\u52a8\u3002\u8981\u770b\u91cd\u590d\u4f1a\u600e\u6837\u5f52\u7c7b\uff0c\u5e94\u5148\u8dd1\u539f\u59cb\u86cb\u767d\u96c6\uff0c\u4ece\u8be6\u7ec6\u8868\u9009\u51fa\u786e\u5b9e\u5c5e\u4e8e\u5b8c\u6574\u5355\u62f7\u8d1d\u3001\u4e14\u53ea\u547d\u4e2d\u4e00\u4e2a\u6807\u8bb0\u7684\u86cb\u767d\u3002\u590d\u5236\u65f6\u53ea\u6539\u8bb0\u5f55\u540d\uff0c\u6c28\u57fa\u9178\u5e8f\u5217\u9010\u5b57\u4fdd\u7559\u3002\u5982\u6b64\u4e00\u6765\uff0c\u82e5\u603b\u5b8c\u6574\u5ea6\u6ca1\u6709\u53d8\u5316\uff0c\u4fbf\u80fd\u533a\u5206\u662f\u65b0\u8bb0\u5f55\u6ca1\u6709\u547d\u4e2d\uff0c\u8fd8\u662f\u547d\u4e2d\u4e86\u4f46\u4ece\u5355\u62f7\u8d1d\u8f6c\u6210\u4e86\u91cd\u590d\u3002<\/p>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u4e09\u6b21\u4f7f\u7528\u540c\u4e00\u5957 124 \u4e2a\u7ec6\u83cc\u6807\u8bb0<\/h2>\n

\u8fd9\u6b21\u5b89\u88c5\u7684\u662f BUSCO 5.5.0\uff0c\u4f7f\u7528\u65e5\u671f\u4e3a 2024 \u5e74 1 \u6708 8 \u65e5\u7684 bacteria_odb10<\/code>\uff0c\u79bb\u7ebf\u8fd0\u884c\u86cb\u767d\u6a21\u5f0f\u3002\u539f\u59cb FASTA \u6709\u56db\u5343\u4e09\u767e\u4e2a\u4e0d\u540c\u7684\u86cb\u767d\u7f16\u53f7\uff0c\u7a0b\u5e8f\u5728\u4e00\u767e\u4e8c\u5341\u56db\u4e2a\u6807\u8bb0\u4e0a\u5168\u90e8\u627e\u5230\u5b8c\u6574\u5355\u62f7\u8d1d\u3002\u7b2c\u4e8c\u6b21\u4ece\u57fa\u7ebf\u8be6\u8868\u53d6\u4e09\u6761\u547d\u4e2d\u86cb\u767d\uff0c\u5404\u590d\u5236\u4e00\u4efd\uff0c\u4fdd\u7559\u539f\u4ef6\uff0c\u5e76\u7ed9\u65b0\u7f16\u53f7\u52a0\u540e\u7f00\u3002\u7b2c\u4e09\u6b21\u5728\u8fd9\u4e2a\u6587\u4ef6\u7684\u57fa\u7840\u4e0a\uff0c\u53e6\u5916\u5220\u9664\u4e00\u4e2a\u5148\u524d\u5b8c\u6574\u5355\u62f7\u8d1d\u6807\u8bb0\u5bf9\u5e94\u7684\u552f\u4e00\u8f93\u5165\u86cb\u767d\u3002\u4e09\u6b21\u90fd\u4ece\u5934\u8fd0\u884c\u641c\u7d22\uff0c\u6ca1\u6709\u6539\u5199\u62a5\u544a\u6570\u5b57\u3002BUSCO \u8c31\u7cfb\u6570\u636e\u76ee\u5f55\u4e0e\u7248\u672c\u8bb0\u5f55<\/a><\/p>\n

\n\n\n\n\n\n\n\n
\u8f93\u5165\u86cb\u767d<\/th>\n\u6761\u76ee\u6570<\/th>\n\u5b8c\u6574\u5355\u62f7\u8d1d<\/th>\n\u5b8c\u6574\u91cd\u590d<\/th>\n\u7f3a\u5931<\/th>\n\u5b8c\u6574\u5ea6<\/th>\n<\/tr>\n<\/thead>\n
\u539f\u59cb\u516c\u5f00\u86cb\u767d\u96c6<\/td>\n4300<\/td>\n124<\/td>\n0<\/td>\n0<\/td>\n100.0%<\/td>\n<\/tr>\n
\u4e3a\u4e09\u4e2a\u6807\u8bb0\u5404\u6dfb\u4e00\u4efd\u76f8\u540c\u5e8f\u5217<\/td>\n4303<\/td>\n121<\/td>\n3<\/td>\n0<\/td>\n100.0%<\/td>\n<\/tr>\n
\u518d\u5220\u9664\u7b2c\u56db\u4e2a\u6807\u8bb0\u7684\u86cb\u767d<\/td>\n4302<\/td>\n120<\/td>\n3<\/td>\n1<\/td>\n99.2%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u7b2c\u4e00\u773c\u770b\u6700\u540e\u4e00\u884c\u5f88\u5bb9\u6613\u5ffd\u7565\u4e00\u4ef6\u4e8b\uff0c\u8f93\u5165\u4ecd\u7136\u6bd4\u539f\u59cb\u86cb\u767d\u96c6\u591a\u4e24\u6761\uff0c\u5b8c\u6574\u5ea6\u5374\u4e0b\u964d\u4e86\u3002\u65b0\u589e\u7684\u4e09\u6761\u5168\u90e8\u843d\u5728\u5df2\u6709\u6807\u8bb0\u4e0a\uff0c\u8865\u4e0d\u56de\u53e6\u4e00\u4e2a\u6807\u8bb0\u3002\u8fd9\u91cc\u7684\u91cd\u590d\u7387\u7ea6\u4e3a\u767e\u5206\u4e4b\u4e8c\u70b9\u56db\uff0c\u5206\u5b50\u662f\u4e09\u4e2a\u5206\u7c7b\u4e3a\u91cd\u590d\u7684\u6807\u8bb0\uff0c\u5206\u6bcd\u662f\u4e00\u767e\u4e8c\u5341\u56db\u4e2a\u9884\u671f\u6807\u8bb0\u3002\u7528\u65b0\u589e\u7684\u4e09\u6761\u53bb\u9664\u56db\u5343\u591a\u6761\u8f93\u5165\u86cb\u767d\u7b97\u53e6\u4e00\u79cd\u6bd4\u4f8b\uff0c\u4e24\u79cd\u6570\u503c\u4f1a\u5dee\u5f88\u8fdc\u3002\u628a\u5206\u5b50\u3001\u5206\u6bcd\u548c\u6bcf\u4e2a\u6807\u8bb0\u7684\u72b6\u6001\u5199\u5168\uff0c\u8bfb\u8005\u624d\u80fd\u91cd\u7b97\u3002<\/p>\n

\"\u5b8c\u6574\u5ea6\u76f8\u540c\uff0c\u62f7\u8d1d\u6570\u4e0d\u540c\u3002\u5728
\u5728 4300 \u6761\u5927\u80a0\u6746\u83cc\u86cb\u767d\u4e2d\u590d\u5236\u4e09\u6761\uff0c\u518d\u5220\u9664\u7b2c\u56db\u4e2a\u6807\u8bb0\u3002<\/figcaption><\/figure>\n

\u4ece\u767e\u5206\u6bd4\u627e\u56de\u5177\u4f53\u86cb\u767d<\/h2>\n

\u4ee5 1009041at2<\/code> \u4e3a\u4f8b\u3002\u539f\u59cb\u62a5\u544a\u628a\u7ec6\u80de\u5206\u88c2\u86cb\u767d FtsZ \u7684 NP_414637.1<\/code> \u8bb0\u4e3a\u5b8c\u6574\uff0c\u5f97\u5206 260.1\uff0c\u547d\u4e2d\u957f\u5ea6\u680f\u4e3a 218\u3002\u590d\u5236\u540e\uff0c\u8be6\u7ec6\u8868\u4e2d\u540c\u4e00\u6807\u8bb0\u4e0b\u9762\u6709\u4e24\u884c\uff0c\u5206\u522b\u5bf9\u5e94\u539f\u7f16\u53f7\u548c\u5e26\u540e\u7f00\u7684\u65b0\u7f16\u53f7\uff0c\u4e24\u884c\u7684\u5f97\u5206\u548c\u547d\u4e2d\u957f\u5ea6\u76f8\u540c\u3002\u6c47\u603b\u8868\u5374\u53ea\u591a\u51fa\u4e00\u4e2a\u91cd\u590d\u6807\u8bb0\u3002\u5982\u679c\u4f60\u628a\u8be6\u7ec6\u8868\u6bcf\u4e00\u884c\u90fd\u5f53\u6210\u4e00\u4e2a\u4e0d\u540c\u6807\u8bb0\u8ba1\u6570\uff0c\u91cd\u590d\u6070\u597d\u4f1a\u88ab\u591a\u8ba1\u4e00\u904d\u3002\u6309\u6807\u8bb0\u5206\u7ec4\u4fdd\u7559\u6240\u6709\u86cb\u767d\u7f16\u53f7\uff0c\u518d\u6309\u72b6\u6001\u6c47\u603b\uff0c\u4e24\u5f20\u8868\u5c31\u80fd\u5bf9\u4e0a\u3002<\/p>\n

busco -i proteins.faa -m proteins -l \/data\/bacteria_odb10 \\\r\n  -o sample -c 4 --offline\r\ncat sample\/short_summary*.txt\r\nawk -F '\\t' '$1=="1009041at2"{print $1,$2,$3,$4,$5}' \\\r\n  sample\/run_bacteria_odb10\/full_table.tsv\r\n<\/code><\/pre>\n
\u62ff\u5230\u771f\u5b9e\u91cd\u590d\u5217\u8868\uff0c\u5148\u5728\u57fa\u56e0\u6ce8\u91ca\u91cc\u6309\u86cb\u767d\u7f16\u53f7\u627e\u8f6c\u5f55\u672c\u548c\u57fa\u56e0\u7f16\u53f7\u3002\u82e5\u4e24\u4e2a\u86cb\u767d\u6765\u81ea\u540c\u4e00\u4e2a\u57fa\u56e0\u7684\u4e0d\u540c\u8f6c\u5f55\u672c\uff0c\u628a\u6bcf\u57fa\u56e0\u4ee3\u8868\u8f6c\u5f55\u672c\u5355\u72ec\u5bfc\u51fa\u518d\u8dd1\u4e00\u6b21\uff0c\u5e76\u4fdd\u7559\u539f\u59cb\u5168\u96c6\u7684\u5206\u6570\u3002\u82e5\u5206\u522b\u4f4d\u4e8e\u4e0d\u540c\u533a\u6bb5\uff0c\u5c31\u6bd4\u8f83\u57fa\u56e0\u7ed3\u6784\u3001\u4e24\u4fa7\u90bb\u8fd1\u57fa\u56e0\u548c\u6d4b\u5e8f\u6df1\u5ea6\u3002\u4e24\u6bb5\u51e0\u4e4e\u76f8\u540c\u4e14\u4fa7\u7ffc\u4e5f\u6210\u7247\u91cd\u5408\uff0c\u4e0e\u76f8\u9694\u5f88\u8fdc\u3001\u4fa7\u7ffc\u4e0d\u540c\u7684\u4e24\u4e2a\u57fa\u56e0\uff0c\u9700\u8981\u5b8c\u5168\u4e0d\u540c\u7684\u540e\u7eed\u8bc1\u636e\u3002\u91cd\u590d\u53ef\u4ee5\u6765\u81ea\u771f\u5b9e\u590d\u5236\u3001\u7ec4\u88c5\u5197\u4f59\u6216\u6ce8\u91ca\u6761\u76ee\u7684\u7ec4\u7ec7\u65b9\u5f0f\uff0c\u5355\u51ed\u8be5\u5b57\u6bcd\u5206\u7c7b\u65e0\u6cd5\u51b3\u5b9a\u8be5\u5220\u54ea\u4e00\u6761\u3002<\/p>\n
\u8fd8\u6709\u4e00\u79cd\u5e38\u89c1\u7684\u5bf9\u7167\uff0c\u662f\u628a\u4e24\u7248\u6ce8\u91ca\u4e2d\u72b6\u6001\u90fd\u4e3a\u5b8c\u6574\u5355\u62f7\u8d1d\u7684\u6761\u76ee\u4e5f\u5e76\u6392\u67e5\u770b\u3002\u4e00\u4e2a\u6807\u8bb0\u5728\u7532\u7248\u547d\u4e2d\u4e00\u6761\u957f\u86cb\u767d\uff0c\u5728\u4e59\u7248\u547d\u4e2d\u53e6\u4e00\u6761\u8fb9\u754c\u4e0d\u540c\u7684\u86cb\u767d\uff0c\u4e24\u8fb9\u90fd\u53ef\u80fd\u8fbe\u5230\u6700\u4f4e\u8981\u6c42\u3002\u5982\u679c\u9879\u76ee\u76ee\u7684\u662f\u6539\u8fdb\u67d0\u4e2a\u57fa\u56e0\u5bb6\u65cf\u7684\u7ed3\u6784\uff0c\u5c31\u5e94\u6bd4\u8f83\u6c28\u57fa\u7aef\u3001\u7fa7\u57fa\u7aef\u3001\u529f\u80fd\u7ed3\u6784\u57df\u4ee5\u53ca\u8f6c\u5f55\u672c\u8bc1\u636e\u3002\u5206\u7c7b\u76f8\u540c\u53ea\u8bf4\u660e\u4e24\u7248\u90fd\u8d8a\u8fc7\u8be5\u6807\u8bb0\u7684\u5224\u65ad\u95e8\u69db\uff0c\u86cb\u767d\u8fb9\u754c\u4ecd\u53ef\u80fd\u6709\u503c\u5f97\u5904\u7406\u7684\u5dee\u5f02\u3002<\/p>\n
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\u7f3a\u5931\u6807\u8bb0\u5e94\u8be5\u56de\u5230\u54ea\u4e00\u5c42<\/h2>\n
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