{"id":71452,"date":"2026-11-15T00:55:09","date_gmt":"2026-11-14T16:55:09","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=71452"},"modified":"2026-11-15T01:14:49","modified_gmt":"2026-11-14T17:14:49","slug":"clair3-pileup-full-alignment-global-quality-threshold-ties","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/clair3-pileup-full-alignment-global-quality-threshold-ties\/","title":{"rendered":"Clair3 \u5b9e\u8dd1\uff5c\u53c2\u6570\u5199\u4e86 30%\uff0c\u4e3a\u4ec0\u4e48\u4e8c\u5341\u4e2a\u5019\u9009\u91cc\u53ea\u6709\u4e24\u4e2a\u8fdb\u5165\u7cbe\u67e5\uff1f"},"content":{"rendered":"<div class=\"ysx-method-guide\" style=\"max-width:820px;min-width:0;margin:0 auto;color:#26374a;line-height:1.86;overflow-wrap:anywhere\">\n<p>\u7528 Clair3 \u8c03\u957f\u8bfb\u957f\u5c0f\u53d8\u5f02\u65f6\uff0c\u6709\u4eba\u4f1a\u62ff\u7b2c\u4e00\u9636\u6bb5\u7684\u4f4d\u70b9\u6570\u4e58\u4e00\u4e2a\u6bd4\u4f8b\uff0c\u9884\u4f30\u7b2c\u4e8c\u9636\u6bb5\u4f1a\u7cbe\u67e5\u591a\u5c11\u4e2a\u4f4d\u70b9\u3002\u5728\u6709\u4e8c\u5341\u884c\u8f93\u5165\u3001\u6bd4\u4f8b\u8bbe\u4e3a\u767e\u5206\u4e4b\u4e09\u5341\u7684\u6d4b\u8bd5\u91cc\uff0c\u8fd9\u79cd\u7b97\u6cd5\u4f1a\u7ed9\u51fa\u516d\u4e2a\u3002\u6211\u4eec\u4ece\u4f5c\u8005\u53d1\u5e03\u7684 2.0.5 \u7248\u5b9e\u9645\u8c03\u7528\u8d28\u91cf\u9608\u503c\u8ba1\u7b97\u548c\u5019\u9009\u9009\u62e9\u4e24\u4e2a\u6b65\u9aa4\uff0c\u5f97\u5230\u7684\u5374\u53ef\u4ee5\u662f\u4e24\u4e2a\u3001\u56db\u4e2a\u6216\u516d\u4e2a\u3002\u5dee\u5f02\u80fd\u8ffd\u8e2a\u5230\u5177\u4f53\u4f4d\u70b9\uff0c\u5b9e\u9a8c\u4e5f\u80fd\u89e3\u91ca\u4e3a\u4ec0\u4e48\u53ea\u770b\u547d\u4ee4\u91cc\u7684\u6bd4\u4f8b\u5bb9\u6613\u8bef\u5224\u3002<a style=\"color:#3f609e;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/HKU-BAL\/Clair3\">Clair3 \u5b98\u65b9\u9879\u76ee<\/a><\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5148\u5206\u6e05\u8fd9\u4e2a\u6bd4\u4f8b\u7528\u5728\u54ea\u91cc<\/h2>\n<p>\u957f\u8bfb\u957f\u6bd4\u5bf9\u5728\u591a\u6570\u4f4d\u7f6e\u63d0\u4f9b\u4e00\u81f4\u7684\u78b1\u57fa\u8bc1\u636e\uff0c\u53e6\u4e00\u4e9b\u4f4d\u7f6e\u53ef\u80fd\u6709\u540c\u805a\u7269\u3001\u9644\u8fd1\u63d2\u5165\u7f3a\u5931\u6216\u4e24\u6761\u5355\u500d\u578b\u4ea4\u53e0\u7684\u590d\u6742\u6a21\u5f0f\u3002Clair3 \u5148\u5229\u7528 pileup \u8868\u793a\u4f5c\u521d\u6b65\u5224\u65ad\uff0c\u518d\u628a\u90e8\u5206\u96be\u4ee5\u5224\u65ad\u7684\u4f4d\u7f6e\u4ea4\u7ed9\u80fd\u591f\u5229\u7528\u66f4\u591a\u8bfb\u6bb5\u7ec6\u8282\u7684\u5168\u6bd4\u5bf9\u6a21\u578b\u3002\u9001\u5f80\u540e\u4e00\u9636\u6bb5\uff0c\u662f\u6d41\u7a0b\u9009\u62e9\u5bf9\u54ea\u4e2a\u4f4d\u7f6e\u591a\u770b\u4e00\u904d\u3002\u8fd9\u4e2a\u6bd4\u4f8b\u672c\u8eab\u65e2\u4e0d\u80fd\u89e3\u91ca\u4e3a\u6700\u7ec8\u9633\u6027\u5360\u6bd4\uff0c\u4e5f\u4e0d\u80fd\u62ff\u6765\u8981\u6c42\u6700\u540e\u7684\u53d8\u5f02\u8868\u5fc5\u987b\u5220\u6389\u6216\u7559\u4e0b\u591a\u5c11\u884c\u3002<a style=\"color:#3f609e;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/HKU-BAL\/Clair3\/blob\/v2.0.5\/preprocess\/SelectCandidates.py\">\u4f5c\u8005\u53d1\u5e03\u7684\u5019\u9009\u9009\u62e9\u7a0b\u5e8f<\/a><\/p>\n<p>\u7b2c\u4e00\u9636\u6bb5\u62a5\u53c2\u8003\u578b\u7684\u4f4d\u7f6e\u4e5f\u53ef\u80fd\u503c\u5f97\u591a\u770b\u4e00\u904d\u3002\u5982\u679c\u4e00\u4e2a\u4f4d\u7f6e\u7684\u8bfb\u6bb5\u652f\u6301\u4e0d\u4e00\u81f4\uff0c\u521d\u6b65\u867d\u9009\u4e86\u53c2\u8003\uff0c\u6a21\u578b\u4e5f\u53ef\u80fd\u7ed9\u51fa\u8f83\u4f4e\u7f6e\u4fe1\u5ea6\u3002\u56e0\u6b64\u6d41\u7a0b\u91cc\u5206\u5f00\u5904\u7406\u521d\u5224\u4e3a\u53d8\u5f02\u7684\u4e00\u7ec4\u548c\u521d\u5224\u4e3a\u53c2\u8003\u7684\u4e00\u7ec4\u3002\u6211\u4eec\u7684\u5b9e\u9a8c\u7ed9\u4e24\u7ec4\u5404\u5341\u884c\uff0c\u5206\u522b\u8bbe\u7f6e\u767e\u5206\u4e4b\u4e09\u5341\uff0c\u4fdd\u7559\u8f6f\u4ef6\u539f\u672c\u7684\u8f93\u5165\u683c\u5f0f\u3002\u771f\u5b9e\u6570\u636e\u4e2d\u4e24\u7ec4\u901a\u5e38\u5927\u5c0f\u4e0d\u540c\uff0c\u60f3\u77e5\u9053\u6709\u591a\u5c11\u4f4d\u70b9\u8fdb\u5165\u4e0b\u4e00\u6b65\uff0c\u5e94\u5148\u628a\u8fd9\u4e24\u4e2a\u5206\u6bcd\u5206\u5f00\u8bb0\u5f55\u3002<a style=\"color:#3f609e;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/HKU-BAL\/Clair3\/blob\/v2.0.5\/preprocess\/SelectQual.py\">\u4f5c\u8005\u53d1\u5e03\u7684\u8d28\u91cf\u9608\u503c\u8ba1\u7b97\u7a0b\u5e8f<\/a><\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u56fa\u5b9a\u4e8c\u5341\u4e2a\u4f4d\u7f6e\uff0c\u53ea\u6539\u9608\u503c\u9644\u8fd1\u7684\u540c\u5206\u60c5\u51b5<\/h2>\n<p>\u7b2c\u4e00\u5957\u4eba\u5de5\u8f93\u5165\u4e2d\uff0c\u5341\u4e2a\u521d\u5224\u53d8\u5f02\u7684\u6700\u4f4e\u51e0\u6863\u8d28\u91cf\u4f9d\u6b21\u662f\u516d\u3001\u516b\u3001\u516b\u3001\u516b\uff0c\u5176\u4ed6\u5747\u9ad8\u4e8e\u516b\u3002\u5341\u4e2a\u521d\u5224\u53c2\u8003\u6700\u4f4e\u4e3a\u4e8c\u3001\u56db\u3001\u56db\u3001\u56db\uff0c\u5176\u4ed6\u90fd\u9ad8\u4e8e\u56db\u3002\u7b2c\u4e8c\u5957\u53ea\u628a\u8fd9\u51e0\u4e2a\u5206\u6570\u6253\u6563\uff0c\u53d8\u5f02\u7aef\u6700\u4f4e\u56db\u6863\u6539\u4e3a\u516d\u3001\u4e03\u3001\u516b\u3001\u4e5d\uff0c\u53c2\u8003\u7aef\u6539\u4e3a\u4e8c\u3001\u4e09\u3001\u56db\u3001\u4e94\u3002\u4e24\u5957\u6570\u636e\u7684\u5750\u6807\u3001\u521d\u5224\u7b49\u4f4d\u3001\u57fa\u56e0\u578b\u548c\u6bcf\u7ec4\u884c\u6570\u5b8c\u5168\u76f8\u540c\u3002\u6bcf\u4e2a\u4f4d\u70b9\u95f4\u9694\u4e09\u767e\u78b1\u57fa\uff0c\u4ee5\u4fbf\u4ece\u8f93\u51fa\u533a\u95f4\u72ec\u7acb\u5bf9\u5e94\u56de\u8f93\u5165\u884c\u3002<\/p>\n<p>\u6211\u4eec\u5148\u8ba9 <code>SelectQual<\/code> \u5bf9\u6574\u4efd\u8f93\u5165\u6c42\u9608\u503c\u5e76\u5199\u5165\u9009\u533a\u76ee\u5f55\uff0c\u518d\u6267\u884c <code>SelectCandidates<\/code>\u3002\u4e24\u5957\u8f93\u5165\u7684\u9608\u503c\u90fd\u662f\u53d8\u5f02\u516b\u5206\u3001\u53c2\u8003\u56db\u5206\u3002\u7136\u540e\u7528\u53e6\u4e00\u4e2a\u7a7a\u76ee\u5f55\u8fd0\u884c\u540c\u4e00\u5019\u9009\u6b65\u9aa4\uff0c\u8ba9\u7a0b\u5e8f\u8d70\u6ca1\u6709\u5168\u5c40\u9608\u503c\u6587\u4ef6\u65f6\u6309\u7ec4\u5185\u6392\u540d\u9009\u53d6\u7684\u5206\u652f\u3002\u4e24\u79cd\u8def\u5f84\u90fd\u6765\u81ea\u540c\u4e00\u4efd\u539f\u7248\u7a0b\u5e8f\u3002\u5b9e\u9a8c\u8303\u56f4\u5230\u9001\u5165\u5168\u6bd4\u5bf9\u7684\u5019\u9009\u533a\u95f4\u4e3a\u6b62\uff0c\u672c\u6b21\u4f7f\u7528\u4eba\u5de5\u6784\u9020\u7684\u521d\u5224\u8868\uff0c\u6ca1\u6709\u8fd0\u884c\u795e\u7ecf\u7f51\u7edc\u63a8\u7406\u6216\u8bc4\u4ef7\u6700\u7ec8\u53d8\u5f02\u51c6\u786e\u7387\u3002<\/p>\n<div class=\"guide-table\" style=\"max-width:100%;overflow-x:auto;margin:18px 0\">\n<table style=\"display:table;width:100%;max-width:100%;min-width:0;table-layout:fixed;border-collapse:collapse;font-size:.92em;line-height:1.6\">\n<thead>\n<tr>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#edf3ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u521d\u5224\u8d28\u91cf\u5206\u5e03<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#edf3ff;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">\u5df2\u6709\u5168\u5c40\u9608\u503c\u65f6\uff0c\u53d8\u5f02 \/ \u53c2\u8003\u5165\u9009<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#edf3ff;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">\u65e0\u5168\u5c40\u9608\u503c\u6587\u4ef6\u65f6\uff0c\u53d8\u5f02 \/ \u53c2\u8003\u5165\u9009<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u9608\u503c\u9644\u8fd1\u591a\u4eba\u540c\u5206<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">1 \/ 1<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">3 \/ 3<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u9608\u503c\u9644\u8fd1\u6253\u6563\u4e3a\u4e0d\u540c\u5206\u6570<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">2 \/ 2<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">3 \/ 3<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>\u89e3\u91ca\u6570\u5b57\u65f6\uff0c\u628a\u5341\u4e2a\u53d8\u5f02\u7684\u5206\u503c\u4ece\u5c0f\u5230\u5927\u5199\u4e0b\u6765\u3002\u62ff\u6392\u5728\u524d\u767e\u5206\u4e4b\u4e09\u5341\u7684\u524d\u4e09\u9879\u6c42\u8fb9\u754c\uff0c\u6700\u540e\u4e00\u9879\u662f\u516b\u3002\u8fd9\u4e2a\u7248\u672c\u968f\u540e\u7528\u7684\u662f\u201c\u8d28\u91cf\u4e25\u683c\u4f4e\u4e8e\u516b\u201d\u6765\u9009\u62e9\u6709\u5168\u5c40\u9608\u503c\u65f6\u7684\u5019\u9009\u3002\u7b2c\u4e00\u5957\u91cc\u53ea\u6709\u516d\u5206\u6ee1\u8db3\uff0c\u7b2c\u4e8c\u5957\u6709\u516d\u5206\u548c\u4e03\u5206\u6ee1\u8db3\u3002\u53c2\u8003\u7aef\u5b8c\u5168\u7c7b\u4f3c\u3002\u6ca1\u6709\u5168\u5c40\u9608\u503c\u65f6\u6539\u4e3a\u628a\u8f93\u5165\u6392\u5e8f\u3001\u76f4\u63a5\u622a\u53d6\u524d\u4e09\u9879\uff0c\u56e0\u6b64\u5373\u4fbf\u8fb9\u754c\u4e0a\u591a\u4eba\u540c\u5206\uff0c\u6570\u91cf\u4ecd\u80fd\u53d6\u6ee1\u4e09\u6761\u3002\u540c\u6837\u662f\u767e\u5206\u4e4b\u4e09\u5341\uff0c\u4e00\u4e2a\u5728\u4f30\u8ba1\u5206\u503c\u8fb9\u754c\uff0c\u53e6\u4e00\u4e2a\u5728\u622a\u53d6\u9884\u5b9a\u6761\u6570\uff0c\u9605\u8bfb\u4ee3\u7801\u548c\u67e5\u770b\u8f93\u51fa\u624d\u80fd\u77e5\u9053\u5b9e\u9645\u8d70\u4e86\u54ea\u6761\u8def\u5f84\u3002<\/p>\n<figure class=\"guide-figure\" style=\"margin:24px 0 28px\"><img loading=\"lazy\" decoding=\"async\" src=\"\/wordpress\/wp-content\/uploads\/2026\/11\/clair3-pileup-full-alignment-global-quality-threshold-ties-guide.png\" alt=\"\u5199\u4e86 30%\uff0c\u5b9e\u9645\u9009\u4e86\u591a\u5c11\u3002\u4e24\u7ec4\u5404 10 \u4e2a\u4f4d\u70b9\uff0c\u5355\u72ec\u68c0\u67e5\u521d\u5224\u7ed3\u679c\u5982\u4f55\u8fdb\u5165\u5168\u6bd4\u5bf9\u3002\u5168\u5c40\u9608\u503c\uff0c\u8fb9\u754c\u540c\u5206\u65f6\u53ea\u9009 2 \u4e2a\uff0c\u4f4e\u4e8e\u9608\u503c\u7684\u53d8\u5f02\u4e0e\u53c2\u8003\u5404 1 \u4e2a\u3002\u6253\u6563\u540c\u5206\uff0c\u9608\u503c\u4e0d\u53d8\uff0c\u5165\u9009 4 \u4e2a\uff0c\u4f4e\u4e8e\u9608\u503c\u7684\u53d8\u5f02\u4e0e\u53c2\u8003\u5404 2 \u4e2a\u3002\u5c40\u90e8\u6392\u5e8f\uff0c\u4e24\u7ec4\u5171\u9009 6 \u4e2a\uff0c\u7ec4\u5185\u76f4\u63a5\u622a\u53d6\uff0c\u53d8\u5f02\u4e0e\u53c2\u8003\u5404 3 \u4e2a\u3002\" width=\"1200\" height=\"840\" style=\"display:block;width:100%;height:auto;border-radius:10px\"><figcaption style=\"font-size:14px;line-height:1.6;margin:9px 2px 0;color:#475569\">\u4e24\u7ec4\u5404 10 \u4e2a\u4f4d\u70b9\uff0c\u5355\u72ec\u68c0\u67e5\u521d\u5224\u7ed3\u679c\u5982\u4f55\u8fdb\u5165\u5168\u6bd4\u5bf9\u3002<\/figcaption><\/figure>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u4e3a\u4ec0\u4e48\u5c11\u4e86\u4e00\u4e2a\u5019\u9009\uff0c\u503c\u5f97\u6cbf\u6587\u4ef6\u8ffd\u4e0b\u6765<\/h2>\n<p>\u5148\u533a\u5206\u6ca1\u8fdb\u5165\u7cbe\u67e5\u548c\u6700\u7ec8\u6ca1\u6709\u88ab\u62a5\u544a\u3002\u4e00\u6b21\u6d41\u7a0b\u53ef\u80fd\u7ee7\u7eed\u4fdd\u7559\u5408\u683c\u7684\u7b2c\u4e00\u9636\u6bb5\u8c03\u7528\uff0c\u4f60\u5728\u7cbe\u67e5\u533a\u95f4\u91cc\u6ca1\u770b\u5230\u67d0\u4e2a\u4f4d\u70b9\uff0c\u4e0d\u80fd\u7531\u6b64\u76f4\u63a5\u63a8\u65ad\u6700\u7ec8\u6587\u4ef6\u4f1a\u6ca1\u6709\u8fd9\u884c\u3002\u53cd\u8fc7\u6765\uff0c\u5df2\u7ecf\u8fdb\u5165\u7cbe\u67e5\u4e5f\u53ea\u8868\u793a\u7b97\u6cd5\u4e3a\u5b83\u8ba1\u7b97\u989d\u5916\u8bc1\u636e\uff0c\u672a\u5fc5\u786e\u8ba4\u6709\u53d8\u5f02\u3002\u8ffd\u8e2a\u5355\u4e2a\u4f4d\u70b9\u65f6\u5e94\u540c\u65f6\u67e5\u521d\u5224\u6587\u4ef6\u3001\u9608\u503c\u6587\u4ef6\u3001\u7cbe\u67e5\u533a\u95f4\u3001\u5168\u6bd4\u5bf9\u7ed3\u679c\u548c\u6700\u7ec8\u5408\u5e76\u6587\u4ef6\uff0c\u8bb0\u5f55\u5b83\u5728\u54ea\u4e00\u7ea7\u6539\u53d8\u4e86\u72b6\u6001\u3002\u8fd9\u6837\u624d\u77e5\u9053\u8981\u68c0\u67e5\u8bc1\u636e\u63d0\u53d6\u3001\u8d28\u91cf\u8fb9\u754c\u8fd8\u662f\u5408\u5e76\u8f93\u51fa\u3002<\/p>\n<pre style=\"max-width:100%;box-sizing:border-box;overflow-x:auto;margin:18px 0;padding:14px 16px;background:#f3f6fa;color:#1a293c;border:1px solid #d8e1ea;border-radius:8px;line-height:1.55;white-space:pre;overflow-wrap:normal;word-break:normal\"><code class=\"language-bash\">mkdir -p selected\r\ngzip -dc pileup.vcf.gz | python3 clair3.py SelectQual \\\r\n  --output_fn selected --var_pct_full 0.30 --ref_pct_full 0.30\r\ncat selected\/qual\r\npython3 clair3.py SelectCandidates --platform ont --ctgName chr1 \\\r\n  --pileup_vcf_fn pileup.vcf.gz --split_folder selected \\\r\n  --var_pct_full 0.30 --ref_pct_full 0.30\r\n<\/code><\/pre>\n<p>\u5bf9\u81ea\u5df1\u7684\u9879\u76ee\uff0c\u5148\u6570\u88ab\u5206\u6790\u67d3\u8272\u4f53\u4e0a\u521d\u5224\u53c2\u8003\u548c\u53d8\u5f02\u7684\u6709\u6548\u6761\u6570\uff0c\u4e3a\u4e24\u7ec4\u5404\u5217\u51fa\u4f4e\u4e8e\u9608\u503c\u3001\u6070\u7b49\u4e8e\u9608\u503c\u4ee5\u53ca\u9ad8\u4e8e\u9608\u503c\u7684\u6570\u91cf\u3002\u82e5\u7b49\u53f7\u5904\u5806\u4e86\u5f88\u591a\u6574\u6570\u5316\u7684\u8d28\u91cf\u503c\uff0c\u8ba1\u7b97\u4e25\u683c\u5c0f\u4e8e\u7684\u6761\u6570\u5e38\u80fd\u76f4\u63a5\u89e3\u91ca\u5dee\u8ddd\u3002\u518d\u786e\u8ba4\u9608\u503c\u662f\u5168\u6837\u672c\u4ea7\u751f\uff0c\u8fd8\u662f\u4ec5\u53d6\u76ee\u6807\u533a\u505a\u4e86\u7f29\u5c0f\u6d4b\u8bd5\u3002\u5355\u4e2a\u67d3\u8272\u4f53\u7684\u5206\u6570\u5206\u5e03\u4e0e\u6574\u4e2a\u6837\u672c\u4e0d\u540c\uff0c\u53ea\u7528\u5b83\u7684\u5c0f\u5206\u5e03\u53bb\u63a8\u5168\u5c40\u5165\u9009\u7387\uff0c\u4f1a\u6df7\u5165\u5206\u5e03\u5dee\u5f02\u3002\u5728\u5df2\u6709\u8fd0\u884c\u76ee\u5f55\u4e2d\u8bd5\u9a8c\u8fd8\u8981\u4e3a\u6bcf\u6b21\u8fd0\u884c\u7528\u72ec\u7acb\u5b50\u76ee\u5f55\uff0c\u65e7\u7684\u9608\u503c\u6587\u4ef6\u672c\u8eab\u5c31\u4f1a\u6539\u53d8\u8fd9\u4e2a\u6b65\u9aa4\u7684\u884c\u4e3a\u3002<\/p>\n<p>\u8bb0\u5f55\u5206\u6570\u65f6\u4fdd\u7559\u539f\u59cb\u5c0f\u6570\u3002\u4eba\u773c\u4ece\u65e5\u5fd7\u91cc\u770b\u5230\u7684\u6574\u6570\u9608\u503c\uff0c\u53ef\u80fd\u53ea\u662f\u7528\u4e8e\u663e\u793a\u7684\u56db\u820d\u4e94\u5165\uff0c\u800c\u7a0b\u5e8f\u5b9e\u9645\u4ece\u9608\u503c\u6587\u4ef6\u8bfb\u56de\u66f4\u7cbe\u786e\u7684\u6d6e\u70b9\u6570\u3002\u82e5\u4e24\u4e2a\u4f4d\u7f6e\u5206\u522b\u843d\u5728\u8fb9\u754c\u7684\u4e24\u4fa7\uff0c\u624b\u6284\u65e5\u5fd7\u4e3a\u6574\u6570\u518d\u4f5c\u6bd4\u8f83\uff0c\u5c31\u4f1a\u5236\u9020\u4e00\u7ec4\u6839\u672c\u4e0d\u5b58\u5728\u7684\u201c\u540c\u5206\u201d\u3002\u672c\u4f8b\u523b\u610f\u7528\u6574\u6570\u65b9\u4fbf\u624b\u7b97\uff0c\u6362\u6210\u771f\u5b9e\u6837\u672c\u65f6\u5e94\u4fdd\u5b58\u539f\u6587\u4ef6\u518d\u505a\u590d\u6838\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 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style=\"color:#3f609e;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/ysx-bioinformatics-practice-074-085-b0103eb15d21.zip\">\u4e0b\u8f7d\u672c\u7bc7\u5b9e\u9a8c\u811a\u672c\u4e0e\u7ed3\u679c\uff0812 \u7bc7\u5408\u96c6 ZIP\uff09<\/a>\uff0c\u89e3\u538b\u540e\u6253\u5f00 <code>077-clair3<\/code> \u76ee\u5f55\u3002<\/p>\n<p class=\"guide-end\" style=\"margin-top:1.6em;padding-top:1.0em;border-top:1px solid #d7e0e9\">\u591a\u6279\u957f\u8bfb\u957f\u9879\u76ee\u9700\u8981\u590d\u6838\u521d\u5224\u5230\u7cbe\u67e5\u7684\u4e2d\u95f4\u6587\u4ef6\u65f6\uff0c\u53ef\u4ee5\u5728<a data-ysx-event=\"contact_click\" style=\"color:#3f609e;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/www.biocloudservice.com\/wordpress\/compute-services\/shared-research-workspace\/\">\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u4fdd\u7559\u6bcf\u6279\u6a21\u578b\u3001\u9608\u503c\u548c\u5019\u9009\u533a\u95f4\uff0c\u65b9\u4fbf\u9010\u4f4d\u70b9\u56de\u770b\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd0\u884c Clair3 \u7684\u4e24\u4e2a\u5019\u9009\u5904\u7406\u6b65\u9aa4\uff0c\u9010\u884c\u6838\u5bf9\u9608\u503c\u3001\u5e76\u5217\u5206\u6570\u548c\u5b9e\u9645\u9001\u5f80\u5168\u6bd4\u5bf9\u7684\u533a\u95f4\u3002<\/p>\n","protected":false},"author":1,"featured_media":71451,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[493,1098,1097,416],"class_list":["post-71452","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-clair3","tag-1098","tag-1097","tag-416"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/clair3-pileup-full-alignment-global-quality-threshold-ties-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/71452","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=71452"}],"version-history":[{"count":2,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/71452\/revisions"}],"predecessor-version":[{"id":71532,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/71452\/revisions\/71532"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/71451"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=71452"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=71452"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=71452"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}