{"id":72614,"date":"2026-11-15T08:16:26","date_gmt":"2026-11-15T00:16:26","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72614"},"modified":"2026-11-15T08:16:26","modified_gmt":"2026-11-15T00:16:26","slug":"metabat2-min-contig-bin-size-unbinned-audit","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/metabat2-min-contig-bin-size-unbinned-audit\/","title":{"rendered":"MetaBAT 2 \u5c11\u8f93\u51fa\u4e00\u4e2a bin\uff0c\u76ee\u6807\u83cc\u5c31\u6ca1\u4e86\u5417\uff1f\u5206\u6e05\u7247\u6bb5\u95e8\u69db\u3001\u805a\u7c7b\u7ed3\u679c\u548c\u8f93\u51fa\u5927\u5c0f"},"content":{"rendered":"
\n

\u5b8f\u57fa\u56e0\u7ec4\u5206\u7bb1\u7ed3\u675f\u540e\uff0c\u8f93\u51fa\u76ee\u5f55\u53ea\u6709\u4e00\u4e2a\u57fa\u56e0\u7ec4\u7bb1\uff0c\u4f46\u5206\u7c7b\u5206\u6790\u663e\u793a\u6837\u672c\u91cc\u8fd8\u6709\u53e6\u4e00\u79cd\u4f18\u52bf\u6210\u5458\u3002\u8fd9\u65f6\u4e0d\u80fd\u76f4\u63a5\u628a\u7f3a\u5c11\u7684\u90a3\u4e2a\u6587\u4ef6\u89e3\u91ca\u6210\u76ee\u6807\u4e0d\u5b58\u5728\u3002\u4e00\u4e2a\u7247\u6bb5\u53ef\u80fd\u592a\u77ed\uff0c\u6ca1\u6709\u8fdb\u5165\u5206\u7bb1\uff0c\u4e5f\u53ef\u80fd\u8fdb\u5165\u4e86\u8ba1\u7b97\u5374\u6ca1\u6709\u83b7\u5f97\u7a33\u5b9a\u5f52\u5c5e\uff0c\u8fd8\u53ef\u80fd\u4e0e\u540c\u6765\u6e90\u7247\u6bb5\u5f62\u6210\u4e86\u8f83\u5c0f\u7684\u96c6\u5408\uff0c\u6700\u540e\u56e0\u8f93\u51fa\u5927\u5c0f\u95e8\u69db\u800c\u6ca1\u6709\u6210\u4e3a\u6587\u4ef6\u3002\u8fd9\u51e0\u79cd\u60c5\u51b5\u90fd\u4f1a\u8ba9\u6700\u7ec8\u76ee\u5f55\u770b\u8d77\u6765\u201c\u5c11\u4e86\u5e8f\u5217\u201d\uff0c\u5e94\u8be5\u9010\u6b65\u8ffd\u67e5\u3002\u6211\u4eec\u7528\u516b\u5341\u4e03\u6761\u5df2\u77e5\u6765\u6e90\u7684\u7247\u6bb5\u548c\u56db\u5217\u8bbe\u8ba1\u6df1\u5ea6\uff0c\u5b9e\u9645\u8dd1\u4e86\u4e09\u6b21 MetaBAT 2\uff0c\u89c2\u5bdf\u4e24\u4e2a\u95e8\u69db\u5206\u522b\u5728\u4ec0\u4e48\u5730\u65b9\u6539\u53d8\u7ed3\u679c\u3002<\/p>\n

\u5148\u7406\u89e3\u5206\u7bb1\u7a76\u7adf\u5229\u7528\u4ec0\u4e48\u4fe1\u606f<\/h2>\n

MetaBAT 2 \u7ed3\u5408\u5e8f\u5217\u7ec4\u6210\u4e0e\u4e0d\u540c\u6837\u672c\u4e2d\u7684\u8986\u76d6\u4fe1\u606f\uff0c\u628a\u53ef\u80fd\u540c\u6765\u6e90\u7684\u7247\u6bb5\u805a\u5728\u4e00\u8d77\u3002\u7ec4\u6210\u4fe1\u606f\u53ef\u4ee5\u5e2e\u52a9\u533a\u5206\u957f\u671f\u6f14\u5316\u5f62\u6210\u7684\u5e8f\u5217\u7279\u5f81\uff0c\u8986\u76d6\u4fe1\u606f\u5219\u5229\u7528\u540c\u4e00\u6210\u5458\u5728\u5404\u6837\u672c\u4e2d\u5f80\u5f80\u4e00\u8d77\u53d8\u5316\u7684\u89c4\u5f8b\u3002\u5047\u8bbe\u7532\u83cc\u5728\u524d\u540e\u4e24\u4e2a\u6837\u672c\u591a\uff0c\u4e2d\u95f4\u6837\u672c\u5c11\uff0c\u6765\u81ea\u7532\u83cc\u7684\u4e0d\u540c\u7247\u6bb5\u53ef\u80fd\u5177\u6709\u76f8\u4f3c\u7684\u53d8\u5316\u65b9\u5411\u3002\u82e5\u53e6\u4e00\u6210\u5458\u5448\u76f8\u53cd\u8d8b\u52bf\uff0c\u591a\u4e2a\u6837\u672c\u5c31\u63d0\u4f9b\u4e86\u53ef\u533a\u5206\u7684\u7ebf\u7d22\u3002\u53ea\u6709\u4e00\u4e2a\u6837\u672c\u65f6\u4ecd\u53ef\u4ee5\u8fd0\u884c\uff0c\u4f46\u5c11\u4e86\u8fd9\u90e8\u5206\u8de8\u6837\u672c\u53d8\u5316\u4fe1\u606f\uff0c\u7ed3\u679c\u53d6\u51b3\u4e8e\u5f53\u524d\u7ec4\u6210\u548c\u6df1\u5ea6\u80fd\u5426\u533a\u5206\u5019\u9009\u7247\u6bb5\u3002MetaBAT 2 \u539f\u59cb\u7814\u7a76<\/a> \u00b7 \u4f5c\u8005\u9879\u76ee\u8bf4\u660e<\/a><\/p>\n

\u8fd9\u4e9b\u89c4\u5f8b\u5b58\u5728\u4f8b\u5916\u3002\u76f8\u8fd1\u83cc\u682a\u53ef\u80fd\u6709\u76f8\u4f3c\u7684\u5e8f\u5217\u7ec4\u6210\u548c\u51e0\u4e4e\u76f8\u540c\u7684\u4e30\u5ea6\u53d8\u5316\uff0c\u8d28\u7c92\u62f7\u8d1d\u6570\u53ef\u80fd\u504f\u79bb\u5bbf\u4e3b\u67d3\u8272\u4f53\uff0c\u91cd\u590d\u533a\u57df\u7684\u56de\u8d34\u6df1\u5ea6\u4e5f\u4f1a\u5f02\u5e38\u3002\u56e0\u6b64\uff0c\u4e00\u4e2a\u7247\u6bb5\u88ab\u5206\u8fdb\u67d0\u4e2a\u7bb1\u9996\u5148\u7ed9\u51fa\u4e86\u53ef\u7ee7\u7eed\u6838\u9a8c\u7684\u5f52\u5c5e\u5047\u8bbe\u3002\u540e\u7eed\u8fd8\u5e94\u68c0\u67e5\u57fa\u56e0\u6807\u8bb0\u3001\u5206\u7c7b\u4e00\u81f4\u6027\u3001\u8986\u76d6\u5f02\u5e38\u4ee5\u53ca\u4e0e\u5176\u4ed6\u7247\u6bb5\u7684\u8fde\u63a5\u3002\u7406\u89e3\u8f93\u5165\u4fe1\u606f\u7684\u6765\u6e90\uff0c\u6709\u52a9\u4e8e\u5224\u65ad\u54ea\u7c7b\u9519\u8bef\u53ef\u4ee5\u9760\u8865\u6837\u672c\u6539\u5584\uff0c\u54ea\u7c7b\u9700\u8981\u957f\u8bfb\u957f\u6216\u66f4\u6709\u8fa8\u522b\u529b\u7684\u5e8f\u5217\u8bc1\u636e\u3002<\/p>\n

\"\u5c11\u4e00\u4e2a
\u6700\u77ed\u7247\u6bb5\u4e0e\u6700\u5c0f\u8f93\u51fa\u7bb1\uff0c\u5206\u4e24\u8f6e\u6539\u53d8<\/figcaption><\/figure>\n

\u5b9e\u8dd1\u7ecf\u9a8c \u00b7 \u7528\u4e24\u79cd\u6765\u6e90\u548c\u56db\u5217\u6df1\u5ea6\u5206\u522b\u6539\u53d8\u95e8\u69db<\/h2>\n

\u5b89\u88c5\u8fd0\u884c\u7684\u662f Ubuntu \u6253\u5305\u7684 MetaBAT 2.15\uff0c\u5305\u7248\u672c\u4e3a 2.15-4\u3002\u8be5\u6784\u5efa\u7684\u5e2e\u52a9\u5934\u90e8\u628a\u7248\u672c\u663e\u793a\u4e3a\u5e26\u6709\u6784\u5efa\u5360\u4f4d\u6807\u8bb0\u7684\u5f62\u5f0f\uff0c\u56e0\u6b64\u590d\u73b0\u8bb0\u5f55\u540c\u65f6\u4fdd\u5b58\u5305\u7248\u672c\u4e0e\u5b8c\u6574\u5e2e\u52a9\u8f93\u51fa\u3002\u8f93\u5165\u7531\u56fa\u5b9a\u79cd\u5b50\u751f\u6210\u3002\u7532\u6765\u6e90\u6709\u56db\u5341\u516d\u6761\u4e03\u5343\u78b1\u57fa\u957f\u7247\u6bb5\u4e0e\u5341\u6761\u4e24\u5343\u78b1\u57fa\u77ed\u7247\u6bb5\uff0c\u4e59\u6765\u6e90\u6709\u4e8c\u5341\u4e00\u6761\u957f\u7247\u6bb5\u4e0e\u5341\u6761\u77ed\u7247\u6bb5\uff0c\u603b\u8ba1\u516b\u5341\u4e03\u6761\u3001\u4e94\u5341\u4e07\u4e5d\u5343\u78b1\u57fa\u3002\u7532\u4e59\u7684\u8bbe\u8ba1\u78b1\u57fa\u7ec4\u6210\u4e0d\u540c\uff0c\u56db\u4e2a\u6837\u672c\u4e2d\u7684\u8bbe\u8ba1\u6df1\u5ea6\u65b9\u5411\u4e5f\u4e0d\u540c\uff0c\u5404\u7247\u6bb5\u52a0\u5165\u5c11\u91cf\u6570\u503c\u6270\u52a8\uff0c\u4ee5\u4fbf\u89c2\u5bdf\u7b97\u6cd5\u786e\u5b9e\u533a\u5206\u4e86\u54ea\u4e00\u4e9b\u8bb0\u5f55\u3002<\/p>\n

\u8fd9\u4efd\u6df1\u5ea6\u6587\u4ef6\u76f4\u63a5\u6309\u8f6f\u4ef6\u8981\u6c42\u5199\u5165\u5747\u503c\u4e0e\u65b9\u5dee\u5217\uff0c\u6ca1\u6709\u4ece\u6bd4\u5bf9\u6587\u4ef6\u4f30\u8ba1\u771f\u5b9e\u6d4b\u5e8f\u8986\u76d6\u3002\u6bcf\u4e2a\u6837\u672c\u6709\u4e00\u5bf9\u6570\u503c\u5217\uff0c\u5148\u5747\u503c\u540e\u65b9\u5dee\uff0c\u9996\u5217\u7247\u6bb5\u6807\u8bc6\u4e0e FASTA \u4e00\u81f4\u3002\u6f14\u793a\u4e2d\u7684\u65b9\u5dee\u56fa\u5b9a\u4e3a\u4e8c\uff0c\u53ea\u662f\u8f93\u5165\u53c2\u6570\u3002\u6211\u4eec\u6ca1\u6709\u5c06\u5b83\u89e3\u91ca\u6210\u67d0\u4e2a\u771f\u5b9e\u6587\u5e93\u7684\u79bb\u6563\u7a0b\u5ea6\u3002\u4eba\u5de5\u8bbe\u8ba1\u7684\u7ec4\u6210\u548c\u6df1\u5ea6\u8ba9\u5bf9\u7167\u5bb9\u6613\u91cd\u8dd1\uff0c\u4e5f\u610f\u5473\u7740\u4e0d\u80fd\u7528\u8fd9\u4e24\u7ec4\u7684\u5206\u79bb\u6548\u679c\u4f30\u7b97\u81ea\u7136\u7fa4\u843d\u7684\u51c6\u786e\u7387\u3002\u8f93\u5165\u751f\u6210\u4e0e\u4e09\u8f6e\u8c03\u7528\u811a\u672c<\/a><\/p>\n

python3 107-metabat2\/run.py "$(command -v metabat2)" work107\r\n# \u9ed8\u8ba4\u5927\u5c0f\u95e8\u69db\u8fd9\u4e00\u8f6e\uff0c\u53e6\u7528\u65b0\u8f93\u51fa\u76ee\u5f55\u6838\u5bf9\r\nmkdir -p verify107\r\nmetabat2 -i work107\/contigs.fa -a work107\/depth.tsv \\\r\n  -o verify107\/bin -m 2500 -s 200000 -t 2 \\\r\n  --seed 317 --noAdd --unbinned\r\n<\/code><\/pre>\n
\u4e09\u8f6e\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u3001\u7ebf\u7a0b\u6570\u548c\u5173\u95ed\u9644\u52a0\u56de\u6536\u6b65\u9aa4\u7684\u8bbe\u7f6e\u3002\u7b2c\u4e00\u8f6e\u7684\u6700\u77ed\u7247\u6bb5\u4e3a\u4e24\u5343\u4e94\u767e\u78b1\u57fa\uff0c\u6700\u5c0f\u8f93\u51fa\u7bb1\u4e3a\u4e8c\u5341\u4e07\u78b1\u57fa\u3002\u7b2c\u4e8c\u8f6e\u53ea\u628a\u6700\u5c0f\u8f93\u51fa\u7bb1\u6539\u4e3a\u5341\u4e07\u3002\u7b2c\u4e09\u8f6e\u4fdd\u7559\u5341\u4e07\u7684\u7bb1\u95e8\u69db\uff0c\u518d\u628a\u6700\u77ed\u7247\u6bb5\u6539\u4e3a\u4e00\u5343\u4e94\u767e\u3002\u660e\u786e\u5173\u95ed\u9644\u52a0\u56de\u6536\uff0c\u53ef\u4ee5\u4f7f\u8fd9\u6b21\u5bf9\u7167\u96c6\u4e2d\u5728\u53c2\u4e0e\u805a\u7c7b\u7684\u7247\u6bb5\u548c\u8f93\u51fa\u95e8\u69db\u3002\u5b9e\u9645\u9879\u76ee\u4f7f\u7528\u5176\u4ed6\u9644\u52a0\u9009\u9879\u65f6\uff0c\u5e94\u91cd\u65b0\u68c0\u67e5\u5176\u5bf9\u5c0f\u7247\u6bb5\u5f52\u5c5e\u7684\u5f71\u54cd\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u6761\u4ef6<\/th>\n\u7532\u6765\u6e90\u5199\u5165\u7684\u957f\u7247\u6bb5\u3001\u77ed\u7247\u6bb5<\/th>\n\u4e59\u6765\u6e90\u5199\u5165\u7684\u957f\u7247\u6bb5\u3001\u77ed\u7247\u6bb5<\/th>\n\u5199\u51fa\u7684\u7bb1\u5927\u5c0f<\/th>\n<\/tr>\n<\/thead>\n
\u6700\u77ed 2,500\uff0c\u7bb1\u81f3\u5c11 200,000<\/td>\n45\u30010<\/td>\n0\u30010<\/td>\n315,000<\/td>\n<\/tr>\n
\u6700\u77ed 2,500\uff0c\u7bb1\u81f3\u5c11 100,000<\/td>\n45\u30010<\/td>\n19\u30010<\/td>\n315,000 \u548c 133,000<\/td>\n<\/tr>\n
\u6700\u77ed 1,500\uff0c\u7bb1\u81f3\u5c11 100,000<\/td>\n46\u30019<\/td>\n21\u30017<\/td>\n340,000 \u548c 161,000<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u6bcf\u4e2a\u8f93\u51fa\u7bb1\u90fd\u6309\u539f\u59cb\u7247\u6bb5\u6807\u8bc6\u6838\u5bf9\u6210\u5458\uff0c\u5e76\u5206\u522b\u7edf\u8ba1\u7532\u4e59\u6765\u6e90\u3002\u4e09\u8f6e\u5b9e\u9645\u5199\u51fa\u7684\u7bb1\u5747\u6ca1\u6709\u6df7\u5408\u4e24\u79cd\u8bbe\u8ba1\u6765\u6e90\u3002\u8fd9\u91cc\u7684\u201c\u6ca1\u6709\u6df7\u5408\u201d\u53ea\u9488\u5bf9\u6211\u4eec\u4e8b\u5148\u77e5\u9053\u7684\u6a21\u62df\u6807\u7b7e\uff0c\u8fd8\u4e0d\u80fd\u4ee3\u8868\u771f\u5b9e\u57fa\u56e0\u7ec4\u5b8c\u6574\u5ea6\u3002\u7532\u6765\u6e90\u5171\u6709\u4e09\u5341\u56db\u4e07\u4e8c\u5343\u78b1\u57fa\uff0c\u7b2c\u4e09\u8f6e\u5199\u51fa\u4e09\u5341\u56db\u4e07\uff0c\u5c11\u7684\u662f\u4e00\u4e2a\u4e24\u5343\u78b1\u57fa\u77ed\u7247\u6bb5\u3002\u4e59\u6765\u6e90\u539f\u6709\u5341\u516d\u4e07\u4e03\u5343\u78b1\u57fa\uff0c\u5199\u51fa\u5341\u516d\u4e07\u4e00\u5343\uff0c\u5c11\u4e86\u4e09\u4e2a\u77ed\u7247\u6bb5\u3002\u628a\u672a\u5199\u5165\u96c6\u5408\u7684\u539f\u59cb\u6807\u8bc6\u7559\u4e0b\uff0c\u5c31\u80fd\u660e\u786e\u590d\u6838\u9057\u6f0f\u7a76\u7adf\u53d1\u751f\u5728\u54ea\u4e9b\u8bb0\u5f55\u4e0a\u3002<\/p>\n
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