{"id":72616,"date":"2026-11-15T08:16:27","date_gmt":"2026-11-15T00:16:27","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72616"},"modified":"2026-11-15T08:16:27","modified_gmt":"2026-11-15T00:16:27","slug":"maxbin2-marker-completeness-reference-fragment-audit","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/maxbin2-marker-completeness-reference-fragment-audit\/","title":{"rendered":"MaxBin 2 \u5df2\u7ecf\u627e\u56de\u6574\u6761\u53c2\u8003\uff0c\u4e3a\u4ec0\u4e48\u5b8c\u6574\u5ea6\u53ea\u6709 98.1%\uff1f\u628a\u6807\u8bb0\u57fa\u56e0\u3001\u7247\u6bb5\u8fc7\u6ee4\u548c\u771f\u5b9e\u8986\u76d6\u5206\u5f00\u6838\u7b97"},"content":{"rendered":"
\n

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\u4ece\u539f\u59cb\u7247\u6bb5\u5230\u8d28\u91cf\u767e\u5206\u6bd4\uff0c\u4e2d\u95f4\u7ecf\u8fc7\u4e86\u54ea\u4e9b\u5224\u65ad<\/h2>\n

MaxBin 2 \u5229\u7528\u7247\u6bb5\u7684\u5e8f\u5217\u7ec4\u6210\u548c\u8986\u76d6\u4e30\u5ea6\u8fdb\u884c\u5206\u7bb1\uff0c\u6807\u8bb0\u57fa\u56e0\u53c8\u53c2\u4e0e\u5019\u9009\u6765\u6e90\u53ca\u8d28\u91cf\u8bc4\u4f30\u3002\u8f93\u5165\u9700\u8981\u6709\u7edf\u4e00\u7684\u7247\u6bb5\u8eab\u4efd\uff0c\u4e30\u5ea6\u53ef\u4ee5\u7531\u539f\u59cb\u8bfb\u6bb5\u56de\u8d34\u4f30\u8ba1\uff0c\u4e5f\u53ef\u4ee5\u6309\u8981\u6c42\u63d0\u4f9b\u73b0\u6210\u7684\u4e30\u5ea6\u6587\u4ef6\u3002\u591a\u4e2a\u6837\u672c\u80fd\u63d0\u4f9b\u540c\u4e00\u6765\u6e90\u968f\u6837\u672c\u5171\u540c\u53d8\u5316\u7684\u7ebf\u7d22\u3002\u6700\u7ec8\u4e00\u4e2a\u7bb1\u5bf9\u5e94\u54ea\u4e9b\u7247\u6bb5\uff0c\u662f\u7531\u8fd9\u4e9b\u4fe1\u606f\u5171\u540c\u5f71\u54cd\u7684\u3002\u56e0\u6b64\u5728\u89e3\u91ca\u7ed3\u679c\u65f6\uff0c\u8981\u540c\u65f6\u4fdd\u7559\u5206\u7ec4\u6210\u5458\u3001\u4e30\u5ea6\u8f93\u5165\u548c\u4f7f\u7528\u7684\u6807\u8bb0\u96c6\u5408\uff0c\u4e0d\u80fd\u53ea\u6709\u6c47\u603b\u767e\u5206\u6bd4\u3002MaxBin 2 \u65b9\u6cd5\u8bba\u6587<\/a> \u00b7 \u4f5c\u8005\u53d1\u5e03\u9875\u4e0e\u8bf4\u660e<\/a><\/p>\n

\u6807\u8bb0\u57fa\u56e0\u4e4b\u6240\u4ee5\u80fd\u4f30\u8ba1\u5b8c\u6574\u5ea6\uff0c\u662f\u56e0\u4e3a\u67d0\u4e9b\u4fdd\u5b88\u57fa\u56e0\u5728\u5bf9\u5e94\u7c7b\u7fa4\u7684\u5b8c\u6574\u57fa\u56e0\u7ec4\u4e2d\u5177\u6709\u53ef\u9884\u6d4b\u7684\u5b58\u5728\u60c5\u51b5\u3002\u5de5\u5177\u80fd\u8bc6\u522b\u5230\u591a\u5c11\uff0c\u5c31\u63d0\u4f9b\u4e86\u5173\u4e8e\u7f3a\u5931\u7a0b\u5ea6\u7684\u7ebf\u7d22\u3002\u5b9e\u9645\u8bc6\u522b\u8fd8\u7ecf\u8fc7\u7f16\u7801\u5e8f\u5217\u9884\u6d4b\u3001\u86cb\u767d\u5339\u914d\u548c\u9608\u503c\u7b5b\u9009\u3002\u7247\u6bb5\u8fb9\u754c\u843d\u5728\u4e00\u4e2a\u57fa\u56e0\u4e2d\u95f4\uff0c\u53ef\u80fd\u5f71\u54cd\u5176\u5b8c\u6574\u8bc6\u522b\uff0c\u5e8f\u5217\u672c\u8eab\u4e0e\u6a21\u578b\u5dee\u5f02\u8f83\u5927\uff0c\u4e5f\u53ef\u80fd\u4f7f\u5339\u914d\u4e0d\u591f\u5145\u5206\u3002\u5373\u4f7f\u53c2\u8003\u5e8f\u5217\u7684\u6bcf\u4e2a\u78b1\u57fa\u90fd\u5728\u8f93\u5165\u91cc\uff0c\u5de5\u5177\u6240\u770b\u5230\u7684\u6807\u8bb0\u4fe1\u53f7\u4ecd\u7136\u53ef\u80fd\u968f\u7740\u7247\u6bb5\u5316\u65b9\u5f0f\u53d1\u751f\u53d8\u5316\u3002<\/p>\n

\"\u5b8c\u6574\u53c2\u8003\u4e3a\u4f55\u53ea\u6709
\u7528\u5df2\u77e5\u53c2\u8003\u56de\u6536\u91cf\u68c0\u9a8c\u6807\u8bb0\u5b8c\u6574\u5ea6\u7684\u542b\u4e49<\/figcaption><\/figure>\n

\u5b9e\u8dd1\u7ecf\u9a8c \u00b7 \u4e24\u6761\u516c\u5f00\u53c2\u8003\u3001\u4e24\u79cd\u5207\u7247\u957f\u5ea6\u548c\u4e24\u5957\u6807\u8bb0<\/h2>\n

\u8fd9\u6b21\u8fd0\u884c MaxBin 2.2.7\uff0c\u4f7f\u7528\u5927\u80a0\u6746\u83cc K-12 MG1655 \u548c\u67af\u8349\u82bd\u5b62\u6746\u83cc 168 \u7684\u516c\u5f00\u53c2\u8003\uff0c\u767b\u5f55\u53f7\u5206\u522b\u4e3a NC_000913.3 \u548c NC_000964.3\u3002\u8bfb\u5165\u7684\u5e8f\u5217\u957f\u5ea6\u5206\u522b\u4e3a\u56db\u767e\u516d\u5341\u56db\u4e07\u4e00\u5343\u516d\u767e\u4e94\u5341\u4e8c\u548c\u56db\u767e\u4e8c\u5341\u4e00\u4e07\u4e94\u5343\u516d\u767e\u96f6\u516d\u78b1\u57fa\u3002\u811a\u672c\u4fdd\u5b58\u4e86\u89c4\u8303\u5316\u5e8f\u5217\u7684\u6821\u9a8c\u503c\uff0c\u65b9\u4fbf\u590d\u8dd1\u65f6\u786e\u8ba4\u53d6\u5230\u7684\u662f\u540c\u4e00\u7248\u78b1\u57fa\u5e8f\u5217\u3002\u4e24\u6761\u53c2\u8003\u90fd\u5b8c\u6574\u8fdb\u5165\u4e86\u5207\u7247\u6b65\u9aa4\uff0c\u4e4b\u95f4\u6ca1\u6709\u4eba\u4e3a\u6316\u53bb\u4efb\u4f55\u533a\u6bb5\u3002\u5927\u80a0\u6746\u83cc\u53c2\u8003\u8bb0\u5f55<\/a> \u00b7 \u67af\u8349\u82bd\u5b62\u6746\u83cc\u53c2\u8003\u8bb0\u5f55<\/a><\/p>\n

\u7b2c\u4e00\u79cd\u8f93\u5165\u4ece\u6bcf\u6761\u53c2\u8003\u5f00\u5934\u4f9d\u6b21\u5207\u51fa\u4e24\u4e07\u78b1\u57fa\u7684\u7247\u6bb5\uff0c\u6700\u540e\u4e0d\u8db3\u4e24\u4e07\u7684\u90e8\u5206\u4fdd\u7559\uff0c\u603b\u8ba1\u56db\u767e\u56db\u5341\u56db\u6761\u3002\u7b2c\u4e8c\u79cd\u6bcf\u4e94\u5343\u78b1\u57fa\u5207\u4e00\u6b21\uff0c\u603b\u8ba1\u4e00\u5343\u4e03\u767e\u4e03\u5341\u4e09\u6761\u3002\u4e24\u79cd\u8f93\u5165\u603b\u957f\u5b8c\u5168\u76f8\u540c\u3002\u4e3a\u4e24\u79cd\u6765\u6e90\u8bbe\u8ba1\u4e24\u5217\u8986\u76d6\uff0c\u5927\u80a0\u6746\u83cc\u5206\u522b\u4e3a\u4e09\u5341\u4e94\u548c\u516b\uff0c\u67af\u8349\u82bd\u5b62\u6746\u83cc\u5206\u522b\u4e3a\u4e03\u548c\u4e09\u5341\u516b\u3002\u76f8\u540c\u6765\u6e90\u7684\u5404\u7247\u6bb5\u4f7f\u7528\u76f8\u540c\u7684\u8bbe\u8ba1\u6570\u503c\u3002\u8fd9\u4e2a\u5b9e\u9a8c\u4ece\u5df2\u77e5\u5e8f\u5217\u548c\u53ef\u63a7\u4e30\u5ea6\u5f00\u59cb\uff0c\u6ca1\u6709\u4ece\u6d4b\u5e8f\u539f\u59cb\u8bfb\u6bb5\u4f30\u8ba1\u8986\u76d6\uff0c\u4e5f\u4e0d\u6d89\u53ca\u771f\u5b9e\u7fa4\u843d\u7ec4\u88c5\u7684\u8bef\u5dee\u3002\u8fd0\u884c\u811a\u672c\u3001\u53c2\u8003\u6821\u9a8c\u503c\u4e0e\u4e09\u8f6e\u7ed3\u679c<\/a><\/p>\n

\u4e09\u8f6e\u5206\u522b\u4f7f\u7528\u4e24\u4e07\u5207\u7247\u52a0\u4e00\u767e\u96f6\u4e03\u5957\u6807\u8bb0\u3001\u4e94\u5343\u5207\u7247\u52a0\u540c\u6837\u6807\u8bb0\uff0c\u4ee5\u53ca\u4e24\u4e07\u5207\u7247\u52a0\u56db\u5341\u5957\u6807\u8bb0\u3002\u6700\u77ed\u53c2\u4e0e\u7247\u6bb5\u5747\u4e3a\u4e00\u5343\u78b1\u57fa\uff0c\u7ebf\u7a0b\u6570\u56fa\u5b9a\u4e3a\u4e8c\u3002\u5b9e\u9645\u901a\u8fc7 MaxBin \u7684\u6b63\u5e38\u547d\u4ee4\u5165\u53e3\u8fdb\u884c\u57fa\u56e0\u9884\u6d4b\u3001\u6807\u8bb0\u641c\u7d22\u548c\u5206\u7bb1\u3002\u4f9d\u8d56\u7a0b\u5e8f\u5305\u542b FragGeneScan\u3001HMMER \u548c Bowtie2\uff0c\u672c\u4f8b\u5df2\u7ecf\u63d0\u4f9b\u4e30\u5ea6\uff0c\u8bfb\u6bb5\u56de\u8d34\u6b65\u9aa4\u65e0\u9700\u91cd\u590d\u6267\u884c\u3002\u5b89\u88c5\u540e\u5148\u68c0\u67e5\u5165\u53e3\u5e2e\u52a9\u548c\u8fd9\u4e9b\u4f9d\u8d56\u80fd\u5426\u6b63\u5e38\u542f\u52a8\uff0c\u518d\u63d0\u4ea4\u5b8c\u6574\u8f93\u5165\uff0c\u80fd\u591f\u8f83\u65e9\u53d1\u73b0\u8def\u5f84\u6216\u8fd0\u884c\u5e93\u7f3a\u5931\u3002<\/p>\n

python3 108-maxbin2\/run.py "$(command -v run_MaxBin.pl)" \\\r\n  EC.fasta BS.fasta work108\r\n\r\n# \u5176\u4e2d\u4e00\u8f6e\u7684\u7b49\u4ef7\u547d\u4ee4\uff0c\u53e6\u9009\u65b0\u7ed3\u679c\u524d\u7f00\r\nrun_MaxBin.pl -contig work108\/chunks20k_107\/assembly.fa \\\r\n  -abund_list work108\/chunks20k_107\/abund_list.txt \\\r\n  -out work108\/recheck -min_contig_length 1000 -markerset 107 -thread 2\r\n<\/code><\/pre>\n
\n\n\n\n\n\n\n\n
\u8f93\u5165\u4e0e\u6807\u8bb0\u8bbe\u7f6e<\/th>\n\u5927\u80a0\u6746\u83cc\u56de\u6536\u78b1\u57fa<\/th>\n\u5927\u80a0\u6746\u83cc\u5b8c\u6574\u5ea6<\/th>\n\u67af\u8349\u82bd\u5b62\u6746\u83cc\u56de\u6536\u78b1\u57fa<\/th>\n\u67af\u8349\u82bd\u5b62\u6746\u83cc\u5b8c\u6574\u5ea6<\/th>\n<\/tr>\n<\/thead>\n
\u4e24\u4e07\u5207\u7247\uff0c107 \u6807\u8bb0<\/td>\n4,641,652<\/td>\n98.1%<\/td>\n4,215,606<\/td>\n100.0%<\/td>\n<\/tr>\n
\u4e94\u5343\u5207\u7247\uff0c107 \u6807\u8bb0<\/td>\n4,641,652<\/td>\n97.2%<\/td>\n4,215,000<\/td>\n96.3%<\/td>\n<\/tr>\n
\u4e24\u4e07\u5207\u7247\uff0c40 \u6807\u8bb0<\/td>\n4,641,652<\/td>\n97.5%<\/td>\n4,215,606<\/td>\n100.0%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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