{"id":72658,"date":"2026-11-15T08:38:01","date_gmt":"2026-11-15T00:38:01","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72658"},"modified":"2026-11-15T08:48:45","modified_gmt":"2026-11-15T00:48:45","slug":"das-tool-bin-score-threshold-candidate-integration","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/das-tool-bin-score-threshold-candidate-integration\/","title":{"rendered":"DAS Tool \u600e\u6837\u6574\u5408\u591a\u4efd\u5206\u7bb1\u7ed3\u679c\uff1f\u5b9e\u8dd1\u6bd4\u8f83\u5206\u6570\u9608\u503c\uff0c\u5e76\u8ffd\u8e2a\u6bcf\u4e00\u6761\u4fdd\u7559\u4e0b\u6765\u7684\u7247\u6bb5"},"content":{"rendered":"
\n

\u540c\u4e00\u4efd\u5b8f\u57fa\u56e0\u7ec4\u7ec4\u88c5\u4ea4\u7ed9\u51e0\u4e2a\u5206\u7bb1\u5de5\u5177\uff0c\u5e38\u4f1a\u5f97\u5230\u6570\u91cf\u3001\u6210\u5458\u548c\u8d28\u91cf\u5404\u4e0d\u76f8\u540c\u7684\u5019\u9009\u7bb1\u3002\u67d0\u4e2a\u5de5\u5177\u628a\u51e0\u6761\u7247\u6bb5\u653e\u5728\u4e00\u8d77\uff0c\u53e6\u4e00\u4e2a\u5de5\u5177\u628a\u5b83\u4eec\u62c6\u5f00\uff0c\u8fd8\u6709\u7684\u7247\u6bb5\u5728\u591a\u4efd\u7ed3\u679c\u4e2d\u53cd\u590d\u51fa\u73b0\u3002\u6574\u5408\u4e4b\u524d\u9996\u5148\u9700\u8981\u786e\u5b9a\u8fd9\u4e9b\u6587\u4ef6\u662f\u4e0d\u662f\u63cf\u8ff0\u540c\u4e00\u6279\u5e8f\u5217\uff0c\u5176\u6b21\u624d\u662f\u9009\u62e9\u54ea\u4e9b\u5019\u9009\u66f4\u503c\u5f97\u4fdd\u7559\u3002\u6211\u4eec\u7528 DAS Tool \u4f5c\u8005\u63d0\u4f9b\u7684\u56db\u4efd\u793a\u4f8b\u7ed3\u679c\u8dd1\u4e86\u4e09\u4e2a\u5206\u6570\u9608\u503c\uff0c\u4ece\u8f93\u5165\u6210\u5458\u5173\u7cfb\u5230\u6700\u7ec8\u7bb1\u8868\u9010\u9879\u6838\u5bf9\uff0c\u8bf4\u660e\u7bb1\u6570\u589e\u52a0\u6216\u51cf\u5c11\u65f6\uff0c\u7a76\u7adf\u53d1\u751f\u4e86\u4ec0\u4e48\u3002<\/p>\n

\u6574\u5408\u4e4b\u524d\uff0c\u5148\u786e\u8ba4\u6240\u6709\u5de5\u5177\u8c08\u7684\u662f\u540c\u4e00\u6279\u7247\u6bb5<\/h2>\n

DAS Tool \u63a5\u6536\u539f\u59cb\u7247\u6bb5\u5e8f\u5217\u548c\u591a\u4efd\u201c\u7247\u6bb5\u5c5e\u4e8e\u54ea\u4e2a\u7bb1\u201d\u7684\u5bf9\u5e94\u8868\uff0c\u5229\u7528\u5355\u62f7\u8d1d\u6807\u8bb0\u7b49\u4fe1\u606f\u8bc4\u4ef7\u5019\u9009\uff0c\u518d\u8fdb\u884c\u53bb\u5197\u4f59\u3001\u9009\u62e9\u548c\u6574\u5408\u3002\u524d\u63d0\u662f\u4e0d\u540c\u5bf9\u5e94\u8868\u91cc\u7684\u540c\u4e00\u4e2a\u7247\u6bb5\u6807\u8bc6\uff0c\u786e\u5b9e\u6307\u5411\u540c\u4e00\u6761\u5e8f\u5217\u3002\u5982\u679c\u51e0\u4efd\u7ed3\u679c\u5206\u522b\u6765\u81ea\u4e0d\u540c\u7ec4\u88c5\uff0c\u53ea\u662f\u78b0\u5de7\u90fd\u51fa\u73b0\u7c7b\u4f3c\u7f16\u53f7\uff0c\u76f4\u63a5\u628a\u5bf9\u5e94\u8868\u4ea4\u8fdb\u53bb\uff0c\u5c31\u65e0\u6cd5\u5efa\u7acb\u6b63\u786e\u7684\u6210\u5458\u91cd\u53e0\u5173\u7cfb\u3002\u6700\u5f00\u59cb\u5e94\u56fa\u5b9a\u4e00\u4efd FASTA\uff0c\u6838\u5bf9\u6240\u6709\u6210\u5458\u6807\u8bc6\u90fd\u80fd\u5728\u5176\u4e2d\u627e\u5230\uff0c\u5e76\u68c0\u67e5\u6bcf\u4e2a\u539f\u59cb\u6587\u4ef6\u662f\u5426\u542b\u6709\u91cd\u590d\u884c\u3002DAS Tool \u539f\u59cb\u7814\u7a76<\/a> \u00b7 \u4f5c\u8005\u9879\u76ee\u4e0e\u4f7f\u7528\u8bf4\u660e<\/a><\/p>\n

\u5bf9\u4e00\u6761\u7247\u6bb5\u51fa\u73b0\u5728\u591a\u4e2a\u5de5\u5177\u7ed3\u679c\u4e2d\u7684\u60c5\u51b5\uff0c\u53ef\u4ee5\u7ee7\u7eed\u4fdd\u7559\u8fd9\u4e9b\u5019\u9009\u5f52\u5c5e\uff0c\u8ba9\u6574\u5408\u6b65\u9aa4\u8bc4\u4f30\u5b83\u4eec\u4e4b\u95f4\u7684\u91cd\u53e0\u3002\u5bf9\u540c\u4e00\u8f93\u5165\u8868\u91cc\u540c\u4e00\u7247\u6bb5\u91cd\u590d\u51fa\u73b0\uff0c\u5219\u5e94\u5355\u72ec\u68c0\u67e5\u5176\u5bf9\u5e94\u7bb1\u6807\u7b7e\u3002\u5b8c\u5168\u76f8\u540c\u7684\u6210\u5458\u5173\u7cfb\u53ef\u80fd\u6765\u81ea\u6587\u4ef6\u91cd\u590d\u62fc\u63a5\uff0c\u540c\u4e00\u6807\u8bc6\u5bf9\u5e94\u4e24\u4e2a\u7bb1\u5219\u63cf\u8ff0\u53e6\u4e00\u79cd\u6b67\u4e49\u3002\u628a\u8de8\u5de5\u5177\u91cd\u53e0\u4e0e\u5355\u4e2a\u8868\u5185\u90e8\u7684\u91cd\u590d\u5206\u522b\u7edf\u8ba1\uff0c\u80fd\u591f\u907f\u514d\u628a\u591a\u5199\u4e86\u51e0\u884c\u6587\u4ef6\u8bef\u5f53\u6210\u5f97\u5230\u4e86\u66f4\u591a\u72ec\u7acb\u8bc1\u636e\u3002\u8f93\u5165\u884c\u6570\u4e0e\u4e0d\u540c\u7247\u6bb5\u6570\uff0c\u4e5f\u5e94\u59cb\u7ec8\u4f5c\u4e3a\u4e24\u5217\u6307\u6807\u4fdd\u7559\u3002<\/p>\n

\"15
\u56fa\u5b9a\u5019\u9009\u4e0e\u6807\u8bb0\u8bc1\u636e\uff0c\u53ea\u6539\u53d8\u5206\u6570\u95e8\u69db<\/figcaption><\/figure>\n

\u5b9e\u8dd1\u7ecf\u9a8c \u00b7 \u56db\u4efd\u4f5c\u8005\u793a\u4f8b\u548c\u4e09\u4e2a\u5206\u6570\u9608\u503c<\/h2>\n

\u5b89\u88c5\u8fd0\u884c DAS Tool 1.1.7\uff0c\u4f7f\u7528\u8be5\u7248\u672c\u4ed3\u5e93\u968f\u9644\u7684\u4eba\u80a0\u9053\u793a\u4f8b\u7ec4\u88c5\uff0c\u4ee5\u53ca CONCOCT\u3001MaxBin 2\u3001MetaBAT \u548c tetraESOM \u7684\u56db\u4efd\u5019\u9009\u5206\u7bb1\u8868\u3002\u56db\u8868\u5408\u8ba1\u4e24\u5343\u4e00\u767e\u4e03\u5341\u4e03\u6761\u6210\u5458\u5173\u7cfb\uff0c\u6d89\u53ca\u4e00\u5343\u516b\u767e\u96f6\u4e5d\u4e2a\u4e0d\u540c\u7247\u6bb5\uff0c\u5176\u4e2d\u4e09\u767e\u4e94\u5341\u516b\u4e2a\u7247\u6bb5\u51fa\u73b0\u5728\u4e0d\u6b62\u4e00\u4efd\u5019\u9009\u8868\u4e2d\u3002\u53e6\u6709\u5341\u4e2a\u7247\u6bb5\u5728\u793a\u4f8b\u7684 MetaBAT \u5bf9\u5e94\u8868\u5185\u90e8\u91cd\u590d\u51fa\u73b0\u3002\u8fd9\u91cc\u63cf\u8ff0\u7684\u662f\u4f5c\u8005\u968f\u9644\u793a\u4f8b\u6587\u4ef6\u7684\u5b9e\u9645\u5185\u5bb9\uff0c\u4e0d\u80fd\u636e\u6b64\u6982\u62ec\u67d0\u4e2a\u5de5\u5177\u5728\u6240\u6709\u9879\u76ee\u4e2d\u7684\u8f93\u51fa\u4e60\u60ef\u3002<\/p>\n

\u672c\u6b21\u5c06\u4f5c\u8005\u63d0\u4f9b\u7684\u9884\u6d4b\u86cb\u767d\u590d\u5236\u5230\u72ec\u7acb\u5de5\u4f5c\u76ee\u5f55\uff0c\u4ee5\u8fd9\u4e9b\u86cb\u767d\u4f5c\u4e3a\u6b63\u5e38\u5165\u53e3\u7684\u8f93\u5165\uff0c\u5b9e\u9645\u6267\u884c\u5355\u62f7\u8d1d\u57fa\u56e0\u641c\u7d22\u548c\u540e\u7eed\u6574\u5408\u3002DIAMOND \u7248\u672c\u4e3a 2.1.9\uff0c\u6240\u7528\u53c2\u8003\u5e93\u6765\u81ea\u540c\u4e00\u4efd DAS Tool \u7248\u672c\u3002\u6ca1\u6709\u91cd\u65b0\u8fdb\u884c\u57fa\u56e0\u9884\u6d4b\uff0c\u907f\u514d\u628a\u86cb\u767d\u9884\u6d4b\u5dee\u5f02\u6df7\u8fdb\u9608\u503c\u5bf9\u7167\u3002\u4e09\u8f6e\u4fdd\u6301\u7247\u6bb5\u3001\u5019\u9009\u8868\u3001\u9884\u6d4b\u86cb\u767d\u548c\u5176\u4ed6\u53c2\u6570\u4e00\u81f4\uff0c\u53ea\u628a\u4fdd\u7559\u5206\u6570\u95e8\u69db\u8bbe\u4e3a\u96f6\u70b9\u4e94\u3001\u96f6\u70b9\u4e5d\u548c\u96f6\u70b9\u4e8c\u3002\u6bcf\u8f6e\u90fd\u4fdd\u7559\u8f93\u5165\u5019\u9009\u8bc4\u5206\u8868\u4e0e\u6700\u540e\u6210\u5458\u8868\uff0c\u9ed8\u8ba4\u95e8\u69db\u4e00\u8f6e\u8fd8\u5b9e\u9645\u5bfc\u51fa\u4e86\u7bb1 FASTA\u3002\u5b8c\u6574\u9a71\u52a8\u811a\u672c\u4e0e\u5b9e\u6d4b\u8f93\u51fa\u6458\u8981<\/a><\/p>\n

python3 112-dastool\/run.py DAS_Tool work112\r\n\r\n# \u67e5\u770b\u5b9e\u9645\u751f\u6210\u7684\u7ed3\u679c\u8868\r\nhead -n 5 work112\/default\/result_DASTool_summary.tsv\r\nhead -n 5 work112\/default\/result_DASTool_contig2bin.tsv\r\n<\/code><\/pre>\n
\n\n\n\n\n\n\n\n
\u5206\u6570\u95e8\u69db<\/th>\n\u6700\u540e\u4fdd\u7559\u7684\u7bb1<\/th>\n\u5199\u5165\u7ed3\u679c\u7684\u4e0d\u540c\u7247\u6bb5<\/th>\n\u540c\u4e00\u7247\u6bb5\u91cd\u590d\u5206\u914d<\/th>\n<\/tr>\n<\/thead>\n
0.5<\/td>\n15<\/td>\n1,498<\/td>\n0<\/td>\n<\/tr>\n
0.9<\/td>\n9<\/td>\n464<\/td>\n0<\/td>\n<\/tr>\n
0.2<\/td>\n16<\/td>\n1,720<\/td>\n0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u7247\u6bb5\u6570\u6309\u6700\u540e\u6210\u5458\u8868\u7684\u6807\u8bc6\u53bb\u91cd\u540e\u7edf\u8ba1\uff0c\u91cd\u590d\u5206\u914d\u5355\u72ec\u68c0\u67e5\u3002\u4e09\u4e2a\u8f93\u51fa\u5728\u8fd9\u4e2a\u68c0\u67e5\u4e0a\u90fd\u6ca1\u6709\u540c\u4e00\u7247\u6bb5\u8fdb\u5165\u591a\u4e2a\u6700\u7ec8\u7bb1\u3002\u8fd9\u4e2a\u7ed3\u679c\u8bf4\u660e\u6574\u5408\u8f93\u51fa\u5728\u6210\u5458\u5173\u7cfb\u4e0a\u8fbe\u5230\u4e86\u4e92\u65a5\u8981\u6c42\uff0c\u8fd8\u4e0d\u80fd\u8bf4\u660e\u6bcf\u6761\u7247\u6bb5\u90fd\u88ab\u5206\u5230\u4e86\u751f\u7269\u5b66\u4e0a\u7684\u6b63\u786e\u6765\u6e90\u3002\u6211\u4eec\u6ca1\u6709\u8fd9\u4e2a\u5b9e\u9645\u7fa4\u843d\u7684\u9010\u7247\u6bb5\u771f\u503c\uff0c\u56e0\u800c\u5c06\u5bf9\u7167\u89e3\u91ca\u9650\u5236\u5728\u5019\u9009\u7b5b\u9009\u3001\u8f93\u51fa\u7ec4\u6210\u53ca\u6807\u8bb0\u8bc4\u5206\u7684\u53d8\u5316\u4e0a\u3002\u5bf9\u771f\u5b9e\u6765\u6e90\u7684\u8fdb\u4e00\u6b65\u5224\u65ad\u8fd8\u9700\u8981\u72ec\u7acb\u5206\u7c7b\u3001\u8d28\u91cf\u8bc4\u4f30\u548c\u8bfb\u6bb5\u8bc1\u636e\u3002<\/p>\n
\u95e8\u69db\u964d\u5230\u96f6\u70b9\u4e8c\u4ee5\u540e\uff0c\u5177\u4f53\u591a\u51fa\u4e86\u4ec0\u4e48<\/h2>\n
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