{"id":72726,"date":"2026-11-15T10:12:53","date_gmt":"2026-11-15T02:12:53","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72726"},"modified":"2026-11-15T10:14:28","modified_gmt":"2026-11-15T02:14:28","slug":"anvio-contig-split-collection-import-coverage-audit","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/anvio-contig-split-collection-import-coverage-audit\/","title":{"rendered":"anvi\u2019o \u5bfc\u5165\u5206\u7bb1\u4e3a\u4ec0\u4e48\u63d0\u793a\u540d\u79f0\u4e0d\u5339\u914d\uff1f\u4ece\u4e24\u4e2a contig\u3001\u4e09\u4e2a split \u5230\u53ef\u6838\u5bf9\u7684\u7ed3\u679c\u6c47\u603b"},"content":{"rendered":"
\n

\u628a\u5916\u90e8\u5206\u7bb1\u7ed3\u679c\u5bfc\u5165 anvi\u2019o \u65f6\uff0c\u4e00\u4e2a\u5e38\u89c1\u95ee\u9898\u662f\u7247\u6bb5\u540d\u79f0\u660e\u660e\u6765\u81ea\u540c\u4e00\u4efd\u7ec4\u88c5\uff0c\u7a0b\u5e8f\u5374\u63d0\u793a\u540d\u79f0\u65e0\u6cd5\u5339\u914d\u3002\u6392\u67e5\u65f6\uff0c\u9700\u8981\u540c\u65f6\u7406\u89e3\u539f\u59cb\u7247\u6bb5\u3001\u6570\u636e\u5e93\u4e2d\u7684\u5206\u5272\u5355\u5143\u548c\u5206\u7bb1\u96c6\u5408\u4e09\u8005\u4e4b\u95f4\u7684\u5173\u7cfb\u3002\u638c\u63e1\u8fd9\u5c42\u6620\u5c04\u4ee5\u540e\uff0c\u8986\u76d6\u7edf\u8ba1\u3001\u4eba\u5de5\u8c03\u6574\u548c\u6700\u7ec8\u5e8f\u5217\u5bfc\u51fa\u90fd\u66f4\u5bb9\u6613\u6838\u5bf9\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u5b89\u88c5 anvi\u2019o 9\uff0c\u7528\u4e00\u6bb5\u516c\u5f00\u53c2\u8003\u5e8f\u5217\u6784\u9020\u4e24\u4e2a\u7247\u6bb5\uff0c\u518d\u751f\u6210\u4e5d\u767e\u6761\u53ef\u7cbe\u786e\u6838\u7b97\u7684\u6bd4\u5bf9\u8bb0\u5f55\uff0c\u5b8c\u6210\u5efa\u5e93\u3001\u8986\u76d6\u5206\u6790\u3001\u5206\u7bb1\u5bfc\u5165\u3001\u96c6\u5408\u5bfc\u51fa\u548c\u5b8c\u6574\u7ed3\u679c\u6c47\u603b\u3002\u5b9e\u9a8c\u4fdd\u5b58\u4e86\u771f\u5b9e\u7684\u5206\u5272\u5750\u6807\u3001\u5e73\u5747\u8986\u76d6\u6df1\u5ea6\u53ca\u6700\u7ec8\u5bfc\u51fa\u5e8f\u5217\u7684\u6821\u9a8c\u7ed3\u679c\uff0c\u8bfb\u8005\u53ef\u4ee5\u6cbf\u7740\u540c\u4e00\u5957\u6587\u4ef6\u9010\u9879\u590d\u67e5\u3002<\/p>\n

\"\u4e24\u4e2a\u7247\u6bb5\u4e3a\u4f55\u5bfc\u51fa\u4e09\u884c\u5f52\u5c5e\u3002anvi\u2019o
anvi\u2019o 9 \u5efa\u5e93\u3001\u8986\u76d6\u3001\u96c6\u5408\u4e0e\u5e8f\u5217\u6838\u5bf9<\/figcaption><\/figure>\n

\u4e09\u7c7b\u6587\u4ef6\u5404\u81ea\u4fdd\u5b58\u4ec0\u4e48\u4fe1\u606f<\/h2>\n

\u5e8f\u5217\u6570\u636e\u5e93\u4fdd\u5b58\u7247\u6bb5\u3001\u57fa\u56e0\u9884\u6d4b\u3001\u5e8f\u5217\u7ec4\u6210\u4ee5\u53ca\u540e\u7eed\u53ef\u4ee5\u52a0\u5165\u7684\u6ce8\u91ca\u3002\u8986\u76d6\u6570\u636e\u5e93\u4fdd\u5b58\u6837\u672c\u7684\u6bd4\u5bf9\u7edf\u8ba1\u548c\u76f8\u5173\u5206\u6790\u7ed3\u679c\u3002\u5206\u7bb1\u96c6\u5408\u5219\u63cf\u8ff0\u54ea\u4e9b\u9879\u76ee\u5c5e\u4e8e\u540c\u4e00\u4e2a\u7bb1\u3002\u628a\u5b83\u4eec\u914d\u5408\u4f7f\u7528\uff0c\u53ef\u4ee5\u5c06\u5916\u90e8\u81ea\u52a8\u5206\u7bb1\u7ed3\u679c\u4e0e\u8986\u76d6\u3001\u5e8f\u5217\u7ec4\u6210\u7b49\u8bc1\u636e\u653e\u5230\u540c\u4e00\u4e2a\u5206\u6790\u73af\u5883\u4e2d\u3002\u5b98\u65b9\u5e8f\u5217\u6570\u636e\u5e93\u8bf4\u660e<\/a><\/p>\n

\u5176\u4e2d\u5bb9\u6613\u6df7\u6dc6\u7684\u662f\uff0c\u6570\u636e\u5e93\u4f1a\u628a\u8f83\u957f\u7247\u6bb5\u7ec4\u7ec7\u6210\u66f4\u5c0f\u7684\u5206\u5272\u5355\u5143\uff0c\u4e5f\u5c31\u662f split\u3002\u5916\u90e8\u5206\u7bb1\u5668\u901a\u5e38\u6309\u539f\u59cb\u7247\u6bb5\u8f93\u51fa\u5f52\u5c5e\u8868\uff0c\u800c anvi\u2019o \u7684\u96c6\u5408\u7ecf\u5e38\u4ee5\u8fd9\u4e9b\u5206\u5272\u5355\u5143\u4e3a\u8bb0\u5f55\u5bf9\u8c61\u3002\u56e0\u6b64\uff0c\u4e00\u4e2a\u539f\u59cb\u7247\u6bb5\u53ef\u4ee5\u5bf9\u5e94\u591a\u884c\u96c6\u5408\u8bb0\u5f55\u3002<\/p>\n

\u5efa\u7acb\u81ea\u5df1\u7684\u6587\u4ef6\u76ee\u5f55\u65f6\uff0c\u53ef\u4ee5\u628a\u53c2\u8003\u5e8f\u5217\u3001\u5e8f\u5217\u6570\u636e\u5e93\u3001\u6bcf\u4e2a\u6837\u672c\u7684\u8986\u76d6\u6570\u636e\u5e93\u548c\u96c6\u5408\u5f52\u5c5e\u8868\u5206\u522b\u4fdd\u5b58\uff0c\u5e76\u7528\u540c\u4e00\u4efd\u9879\u76ee\u8bb0\u5f55\u5173\u8054\u8d77\u6765\u3002\u6587\u4ef6\u540d\u53ef\u4ee5\u5e2e\u52a9\u8bc6\u522b\u7528\u9014\uff0c\u5b9e\u9645\u5bf9\u5e94\u5173\u7cfb\u8fd8\u5e94\u7531\u53c2\u8003\u6821\u9a8c\u503c\u3001\u6570\u636e\u5e93\u4fe1\u606f\u548c\u539f\u59cb\u540d\u79f0\u6620\u5c04\u5171\u540c\u786e\u8ba4\u3002<\/p>\n

\u672c\u6b21\u5b89\u88c5\u4e0e\u8f93\u5165\u600e\u6837\u56fa\u5b9a<\/h2>\n

\u672c\u6b21\u4ece\u5b98\u65b9\u53d1\u5e03\u9875\u5b89\u88c5\u7b2c\u4e5d\u7248\u6e90\u7801\u5305\uff0c\u5728\u72ec\u7acb\u7684 Python 3.10 \u73af\u5883\u4e2d\u8fd0\u884c\uff0c\u8bfb\u53d6\u6bd4\u5bf9\u6587\u4ef6\u7684 pysam \u7248\u672c\u4e3a 0.24.1\u3002\u8f6f\u4ef6\u5305\u9875\u9762\u548c\u5f53\u524d\u5f00\u53d1\u6587\u6863\u7684\u5185\u5bb9\u53ef\u80fd\u7ee7\u7eed\u53d8\u5316\uff0c\u590d\u73b0\u65f6\u5e94\u540c\u65f6\u8bb0\u5f55\u5b9e\u9645\u7248\u672c\u4e0e\u8fd0\u884c\u5e2e\u52a9\u8f93\u51fa\u3002\u5b98\u65b9 Linux \u5b89\u88c5\u8bf4\u660e<\/a><\/p>\n

\u5b9e\u9a8c\u4f7f\u7528\u5927\u80a0\u6746\u83cc\u53c2\u8003\u5e8f\u5217 NC_000913.3<\/code> \u7684\u524d\u516d\u4e07\u78b1\u57fa\u3002\u524d\u56db\u4e07\u4e94\u5343\u78b1\u57fa\u547d\u540d\u4e3a contig_A<\/code>\uff0c\u540e\u9762\u4e00\u4e07\u4e94\u5343\u78b1\u57fa\u547d\u540d\u4e3a contig_B<\/code>\u3002\u4e24\u4e2a\u7247\u6bb5\u90fd\u6765\u81ea\u56fa\u5b9a\u533a\u95f4\uff0c\u5185\u5bb9\u548c\u540d\u79f0\u5728\u6574\u4e2a\u5b9e\u9a8c\u4e2d\u4fdd\u6301\u4e00\u81f4\u3002<\/p>\n

\u8986\u76d6\u8f93\u5165\u4e13\u95e8\u8bbe\u8ba1\u4e3a\u53ef\u624b\u7b97\u7684\u6570\u636e\u3002\u7532\u7247\u6bb5\u6bcf\u4e00\u767e\u78b1\u57fa\u653e\u7f6e\u4e00\u6761\u5b8c\u6574\u5339\u914d\u8bfb\u6bb5\uff0c\u5171\u56db\u767e\u4e94\u5341\u6761\uff0c\u56e0\u6b64\u8986\u76d6\u6df1\u5ea6\u4e3a\u4e00\u3002\u4e59\u7247\u6bb5\u6bcf\u4e00\u767e\u78b1\u57fa\u653e\u7f6e\u4e09\u6761\u5b8c\u6574\u5339\u914d\u8bfb\u6bb5\uff0c\u4e5f\u6709\u56db\u767e\u4e94\u5341\u6761\uff0c\u8986\u76d6\u6df1\u5ea6\u4e3a\u4e09\u3002\u4e24\u8005\u6240\u6709\u4f4d\u7f6e\u90fd\u6709\u8986\u76d6\uff0c\u5408\u8ba1\u4e5d\u767e\u6761\u8bfb\u6bb5\u3002<\/p>\n

\u8fd9\u4e9b\u6570\u636e\u7528\u6765\u9a8c\u8bc1\u6570\u636e\u5e93\u548c\u96c6\u5408\u7684\u5bf9\u5e94\u5173\u7cfb\u3002\u5b9e\u9645\u5b8f\u57fa\u56e0\u7ec4\u5206\u6790\u8fd8\u4f1a\u9047\u5230\u9519\u914d\u3001\u4f4e\u8986\u76d6\u3001\u91cd\u590d\u533a\u57df\u53ca\u591a\u91cd\u6bd4\u5bf9\u7b49\u95ee\u9898\uff0c\u53ef\u4ee5\u5728\u7406\u89e3\u672c\u4f8b\u4e4b\u540e\u7ee7\u7eed\u52a0\u5165\u76f8\u5e94\u8fc7\u6ee4\u548c\u8bca\u65ad\u6b65\u9aa4\u3002<\/p>\n

\u5efa\u5e93\u540e\uff0c\u4e24\u6761\u7247\u6bb5\u5f62\u6210\u4e09\u4e2a split<\/h2>\n

\u672c\u6b21\u4f7f\u7528\u6b63\u5e38\u5efa\u5e93\u547d\u4ee4\uff0c\u542f\u7528\u9ed8\u8ba4\u57fa\u56e0\u9884\u6d4b\uff0c\u5c06\u76ee\u6807\u5206\u5272\u957f\u5ea6\u8bbe\u4e3a\u4e24\u4e07\uff0c\u5e76\u4f7f\u7528\u4e24\u4e2a\u7ebf\u7a0b\u3002\u7a0b\u5e8f\u5b9e\u9645\u9884\u6d4b\u4e86\u4e94\u5341\u516d\u4e2a\u57fa\u56e0\uff0c\u968f\u540e\u751f\u6210\u5e8f\u5217\u6570\u636e\u5e93\u3002\u5b98\u65b9\u5efa\u5e93\u547d\u4ee4\u8bf4\u660e<\/a><\/p>\n

anvi-gen-contigs-database \\\r\n  -f contigs.fna \\\r\n  -o CONTIGS.db \\\r\n  -n collection_practice \\\r\n  -L 20000 \\\r\n  -T 2\r\n<\/code><\/pre>\n
\u6570\u636e\u5e93\u4e2d\u5b9e\u9645\u51fa\u73b0\u4e09\u4e2a\u5206\u5272\u5355\u5143\u3002\u7532\u7247\u6bb5\u88ab\u5206\u6210\u4e24\u90e8\u5206\uff0c\u957f\u5ea6\u5206\u522b\u4e3a\u4e8c\u4e07\u4e8c\u5343\u4e09\u767e\u516b\u5341\u56db\u548c\u4e8c\u4e07\u4e8c\u5343\u516d\u767e\u4e00\u5341\u516d\u78b1\u57fa\u3002\u4e59\u7247\u6bb5\u4fdd\u7559\u4e3a\u4e00\u4e2a\u4e00\u4e07\u4e94\u5343\u78b1\u57fa\u7684\u5355\u5143\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u539f\u59cb\u7247\u6bb5<\/th>\n\u6570\u636e\u5e93\u4e2d\u7684 split<\/th>\n\u8d77\u70b9<\/th>\n\u7ec8\u70b9<\/th>\n\u957f\u5ea6<\/th>\n<\/tr>\n<\/thead>\n
contig_A<\/td>\ncontig_A_split_00001<\/td>\n0<\/td>\n22,384<\/td>\n22,384<\/td>\n<\/tr>\n
contig_A<\/td>\ncontig_A_split_00002<\/td>\n22,384<\/td>\n45,000<\/td>\n22,616<\/td>\n<\/tr>\n
contig_B<\/td>\ncontig_B_split_00001<\/td>\n0<\/td>\n15,000<\/td>\n15,000<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8868\u4e2d\u5750\u6807\u6765\u81ea\u6570\u636e\u5e93\uff0c\u91c7\u7528\u4ece\u96f6\u5f00\u59cb\u3001\u7ec8\u70b9\u4e0d\u5305\u542b\u5728\u533a\u95f4\u5185\u7684\u8868\u793a\u65b9\u5f0f\uff0c\u56e0\u6b64\u957f\u5ea6\u53ef\u4ee5\u7528\u7ec8\u70b9\u51cf\u8d77\u70b9\u6838\u5bf9\u3002\u7532\u7684\u4e24\u4e2a\u533a\u95f4\u76f8\u63a5\uff0c\u603b\u957f\u5ea6\u6070\u597d\u56db\u4e07\u4e94\u5343\u78b1\u57fa\uff0c\u4e59\u7684\u533a\u95f4\u8986\u76d6\u5b8c\u6574\u7247\u6bb5\u3002<\/p>\n
\u76ee\u6807\u5206\u5272\u957f\u5ea6\u662f\u7ec4\u7ec7\u7247\u6bb5\u7684\u53c2\u6570\uff0c\u5b9e\u9645\u8f93\u51fa\u8fd8\u4f1a\u53d7\u5230\u5206\u5272\u903b\u8f91\u548c\u57fa\u56e0\u4f4d\u7f6e\u7684\u5f71\u54cd\u3002\u5b98\u65b9\u5efa\u5e93\u8bf4\u660e\u6307\u51fa\uff0c\u9ed8\u8ba4\u5206\u5272\u4f1a\u8003\u8651\u9884\u6d4b\u57fa\u56e0\u7684\u4f4d\u7f6e\u3002\u56e0\u6b64\uff0c\u751f\u6210\u96c6\u5408\u65f6\u5e94\u4f7f\u7528\u7a0b\u5e8f\u5b9e\u9645\u5199\u51fa\u7684\u540d\u79f0\u548c\u5750\u6807\uff0c\u907f\u514d\u6839\u636e\u76ee\u6807\u957f\u5ea6\u81ea\u884c\u731c\u6d4b\u5206\u5272\u6570\u91cf\u6216\u62fc\u51fa\u540d\u79f0\u3002<\/p>\n
\u8986\u76d6\u6570\u636e\u5e93\u5982\u4f55\u4e0e\u8fd9\u4e9b\u5355\u5143\u8fde\u63a5<\/h2>\n
\u63a5\u4e0b\u6765\u5bf9\u6392\u5e8f\u5e76\u5efa\u7acb\u7d22\u5f15\u7684 BAM \u8fd0\u884c\u8986\u76d6\u5206\u6790\u3002\u4e3a\u4e86\u96c6\u4e2d\u68c0\u67e5\u8986\u76d6\u4e0e\u540d\u79f0\u6620\u5c04\uff0c\u672c\u6b21\u5173\u95ed\u5355\u6838\u82f7\u9178\u53d8\u5f02\u5206\u6790\uff0c\u5c06\u6700\u5c0f\u7247\u6bb5\u957f\u5ea6\u8bbe\u4e3a\u4e00\u5343\uff0c\u6837\u672c\u540d\u79f0\u8bbe\u4e3a\u56fa\u5b9a\u503c\u3002\u5b98\u65b9\u8986\u76d6\u5206\u6790\u8bf4\u660e<\/a><\/p>\n
anvi-profile \\\r\n  -i sample.bam \\\r\n  -c CONTIGS.db \\\r\n  -o PROFILE \\\r\n  -S CASE1 \\\r\n  -M 1000 \\\r\n  --skip-SNV-profiling \\\r\n  -T 2\r\n<\/code><\/pre>\n
\u5b9e\u9645\u4fdd\u5b58\u7684\u8986\u76d6\u7ed3\u679c\u4e0e\u8bbe\u8ba1\u4e00\u81f4\u3002\u7532\u7684\u4e24\u4e2a\u5206\u5272\u5355\u5143\u5e73\u5747\u6df1\u5ea6\u5747\u4e3a\u4e00\uff0c\u4e59\u7684\u5206\u5272\u5355\u5143\u5e73\u5747\u6df1\u5ea6\u4e3a\u4e09\uff0c\u4e09\u4e2a\u5355\u5143\u7684\u8986\u76d6\u68c0\u6d4b\u6bd4\u4f8b\u5747\u4e3a\u4e00\u3002\u8fd9\u4e2a\u7ed3\u679c\u8bf4\u660e\u6bd4\u5bf9\u8bb0\u5f55\u3001\u539f\u59cb\u7247\u6bb5\u4e0e\u6570\u636e\u5e93\u5206\u5272\u5355\u5143\u6b63\u786e\u8fde\u63a5\u8d77\u6765\u4e86\u3002<\/p>\n
\u5982\u679c\u5b9e\u9645\u9879\u76ee\u4e2d\u67d0\u4e9b\u5355\u5143\u5b8c\u5168\u6ca1\u6709\u8986\u76d6\uff0c\u5e94\u5148\u68c0\u67e5\u5bf9\u5e94\u7247\u6bb5\u662f\u5426\u5b58\u5728\u4e8e BAM \u53c2\u8003\u5934\u3001\u662f\u5426\u901a\u8fc7\u6700\u5c0f\u957f\u5ea6\u6761\u4ef6\u4ee5\u53ca\u8bfb\u6bb5\u8fc7\u6ee4\u8981\u6c42\uff0c\u518d\u8003\u8651\u771f\u5b9e\u7684\u4f4e\u4e30\u5ea6\u6216\u7f3a\u5931\u3002\u5206\u6790\u987a\u5e8f\u53ef\u4ee5\u4ece\u539f\u59cb\u540d\u79f0\u5bf9\u5e94\u5173\u7cfb\u5f00\u59cb\uff0c\u9010\u6b65\u7f29\u5c0f\u5230\u8986\u76d6\u548c\u751f\u7269\u5b66\u539f\u56e0\u3002<\/p>\n
\u540c\u4e00\u4e2a\u7ec4\u88c5\u5bf9\u5e94\u591a\u4e2a\u6837\u672c\u65f6\uff0c\u6bcf\u4e2a\u6837\u672c\u7684\u8986\u76d6\u5206\u6790\u90fd\u5e94\u4f7f\u7528\u914d\u5957\u7684\u5e8f\u5217\u6570\u636e\u5e93\u3002\u6837\u672c\u4e4b\u95f4\u7684\u540d\u79f0\u6216\u8f93\u5165\u53c2\u8003\u82e5\u5b58\u5728\u5dee\u5f02\uff0c\u9700\u8981\u5148\u786e\u8ba4\u6765\u6e90\uff0c\u518d\u8003\u8651\u5408\u5e76\u8986\u76d6\u6570\u636e\u5e93\u3002\u4fdd\u7559\u6bcf\u6b21\u6bd4\u5bf9\u4f7f\u7528\u7684\u53c2\u8003\u6821\u9a8c\u503c\uff0c\u53ef\u4ee5\u5728\u8fd9\u91cc\u63d0\u4f9b\u76f4\u63a5\u4f9d\u636e\u3002<\/p>\n
\u9519\u8bef\u5bfc\u5165\u4e0e\u6b63\u786e\u5bfc\u5165\u7684\u5b9e\u9645\u5dee\u522b<\/h2>\n
\u6211\u4eec\u51c6\u5907\u4e00\u5f20\u4e24\u884c\u7684\u5916\u90e8\u5206\u7bb1\u8868\uff0c\u628a\u7532\u7247\u6bb5\u653e\u5165\u7532\u7bb1\uff0c\u628a\u4e59\u7247\u6bb5\u653e\u5165\u4e59\u7bb1\u3002\u8868\u4e2d\u7b2c\u4e00\u5217\u91c7\u7528\u539f\u59cb\u7247\u6bb5\u540d\u79f0\uff0c\u7b2c\u4e8c\u5217\u91c7\u7528\u7bb1\u540d\u79f0\u3002<\/p>\n
printf 'contig_A\\tBin_A\\ncontig_B\\tBin_B\\n' > contig_bins.tsv\r\n<\/code><\/pre>\n
\u7b2c\u4e00\u6b21\u5bfc\u5165\u65f6\u4f7f\u7528\u9ed8\u8ba4\u8bbe\u7f6e\uff0c\u7a0b\u5e8f\u8fd4\u56de\u9519\u8bef\u72b6\u6001\uff0c\u65e5\u5fd7\u660e\u786e\u6307\u51fa\u8f93\u5165\u540d\u79f0\u4e0e\u6570\u636e\u5e93\u4e2d\u7684\u9879\u76ee\u540d\u79f0\u6ca1\u6709\u4ea4\u96c6\uff0c\u5e76\u63d0\u793a\u68c0\u67e5\u7247\u6bb5\u6a21\u5f0f\u3002\u8fd9\u4e2a\u9519\u8bef\u6ca1\u6709\u8981\u6c42\u91cd\u65b0\u6bd4\u5bf9\u6570\u636e\uff0c\u539f\u56e0\u53ef\u4ee5\u76f4\u63a5\u4ece\u540d\u79f0\u5c42\u7ea7\u627e\u5230\u3002<\/p>\n
\u7b2c\u4e8c\u6b21\u5bfc\u5165\u52a0\u5165\u7247\u6bb5\u6a21\u5f0f\u53c2\u6570\uff0c\u7a0b\u5e8f\u6210\u529f\u5c06\u539f\u59cb\u7247\u6bb5\u5f52\u5c5e\u8f6c\u6362\u6210\u4e09\u4e2a\u5206\u5272\u5355\u5143\u7684\u5f52\u5c5e\u3002\u7532\u7684\u4e24\u4e2a\u5206\u5272\u5355\u5143\u8fdb\u5165\u540c\u4e00\u4e2a\u7bb1\uff0c\u4e59\u7684\u5206\u5272\u5355\u5143\u8fdb\u5165\u53e6\u4e00\u4e2a\u7bb1\u3002\u5b98\u65b9\u96c6\u5408\u5bfc\u5165\u8bf4\u660e<\/a><\/p>\n
anvi-import-collection contig_bins.tsv \\\r\n  -c CONTIGS.db \\\r\n  -p PROFILE\/PROFILE.db \\\r\n  -C CORRECT \\\r\n  --contigs-mode\r\n<\/code><\/pre>\n
\u968f\u540e\u901a\u8fc7\u6b63\u5e38\u5bfc\u51fa\u547d\u4ee4\u6838\u5bf9\u96c6\u5408\u5185\u5bb9\u3002\u5b9e\u9645\u5bfc\u51fa\u7684\u8868\u6709\u4e09\u884c\uff0c\u7b2c\u4e00\u5217\u662f\u5206\u5272\u5355\u5143\u540d\u79f0\uff0c\u7b2c\u4e8c\u5217\u662f\u7bb1\u540d\u79f0\u3002\u7532\u5bf9\u5e94\u4e24\u884c\uff0c\u4e59\u5bf9\u5e94\u4e00\u884c\uff0c\u4e0e\u6570\u636e\u5e93\u4e2d\u7684\u5206\u5272\u5173\u7cfb\u5b8c\u5168\u4e00\u81f4\u3002<\/p>\n
anvi-export-collection \\\r\n  -p PROFILE\/PROFILE.db \\\r\n  -C CORRECT \\\r\n  -O exported_collection\r\n<\/code><\/pre>\n
\u8fd9\u91cc\u53ef\u4ee5\u8bbe\u7f6e\u4e00\u4e2a\u660e\u786e\u7684\u6838\u5bf9\u76ee\u6807\u3002\u5bf9\u6bcf\u4e2a\u5df2\u7ecf\u5206\u7bb1\u7684\u539f\u59cb\u7247\u6bb5\uff0c\u67e5\u51fa\u5b83\u5728\u6570\u636e\u5e93\u4e2d\u7684\u5168\u90e8\u5206\u5272\u5355\u5143\uff0c\u786e\u8ba4\u8fd9\u4e9b\u5355\u5143\u90fd\u8fdb\u5165\u9884\u671f\u7684\u7bb1\u3002\u5bf9\u5bfc\u51fa\u96c6\u5408\u4e2d\u7684\u6bcf\u4e2a\u5206\u5272\u5355\u5143\uff0c\u518d\u53cd\u5411\u67e5\u627e\u539f\u59cb\u7247\u6bb5\uff0c\u786e\u8ba4\u6ca1\u6709\u591a\u51fa\u6765\u6e90\u4e0d\u660e\u7684\u9879\u76ee\u3002<\/p>\n
\u7ed3\u679c\u6c47\u603b\u600e\u6837\u56de\u5230\u539f\u59cb\u5e8f\u5217<\/h2>\n
\u96c6\u5408\u5bfc\u5165\u4ee5\u540e\uff0c\u672c\u6b21\u8fd0\u884c\u5b8c\u6574\u6c47\u603b\u547d\u4ee4\uff0c\u8f93\u51fa\u6bcf\u4e2a\u7bb1\u7684\u5e8f\u5217\u3001\u8986\u76d6\u7edf\u8ba1\u3001\u957f\u5ea6\u6307\u6807\u548c\u53ef\u6253\u5f00\u7684\u7ed3\u679c\u7d22\u5f15\u3002\u5b98\u65b9\u6c47\u603b\u529f\u80fd\u4e5f\u9002\u5408\u751f\u6210\u4f9b\u534f\u4f5c\u8005\u9605\u8bfb\u7684\u5e73\u9762\u6587\u4ef6\u3002\u5b98\u65b9\u7ed3\u679c\u6c47\u603b\u8bf4\u660e<\/a><\/p>\n
anvi-summarize \\\r\n  -c CONTIGS.db \\\r\n  -p PROFILE\/PROFILE.db \\\r\n  -C CORRECT \\\r\n  -o SUMMARY\r\n<\/code><\/pre>\n
\u5b9e\u9645\u6c47\u603b\u4e2d\uff0c\u7532\u7bb1\u603b\u957f\u5ea6\u4e3a\u56db\u4e07\u4e94\u5343\u78b1\u57fa\uff0c\u5305\u542b\u4e00\u4e2a\u539f\u59cb\u7247\u6bb5\uff0c\u5e73\u5747\u6df1\u5ea6\u4e3a\u4e00\u3002\u4e59\u7bb1\u603b\u957f\u5ea6\u4e3a\u4e00\u4e07\u4e94\u5343\u78b1\u57fa\uff0c\u5305\u542b\u4e00\u4e2a\u539f\u59cb\u7247\u6bb5\uff0c\u5e73\u5747\u6df1\u5ea6\u4e3a\u4e09\u3002\u6211\u4eec\u8bfb\u53d6\u5bfc\u51fa\u7684\u4e24\u4e2a\u5e8f\u5217\u6587\u4ef6\uff0c\u5c06\u5e8f\u5217\u9010\u5b57\u4e0e\u8f93\u5165\u6bd4\u8f83\uff0c\u5747\u5b8c\u5168\u4e00\u81f4\u3002<\/p>\n
\u8fd9\u9879\u6838\u5bf9\u8fd8\u8bf4\u660e\uff0c\u96c6\u5408\u4e2d\u7684\u4e09\u884c\u5206\u5272\u5355\u5143\u5f52\u5c5e\uff0c\u4e0e\u6700\u7ec8\u4e24\u4e2a\u539f\u59cb\u7247\u6bb5\u7684\u5bfc\u51fa\u6570\u91cf\u80fd\u591f\u540c\u65f6\u6210\u7acb\u3002\u7edf\u8ba1\u7ed3\u679c\u65f6\uff0c\u5e94\u6ce8\u660e\u81ea\u5df1\u6570\u7684\u662f\u539f\u59cb\u7247\u6bb5\u3001\u5206\u5272\u5355\u5143\u8fd8\u662f\u7bb1\u3002\u82e5\u5728\u540c\u4e00\u5f20\u62a5\u544a\u91cc\u6df7\u7528\u8fd9\u4e9b\u6570\u91cf\uff0c\u5bb9\u6613\u8ba9\u4eba\u8bef\u4ee5\u4e3a\u5206\u6790\u8fc7\u7a0b\u4e2d\u589e\u52a0\u6216\u4e22\u5931\u4e86\u7247\u6bb5\u3002<\/p>\n
\u672c\u6b21\u6c47\u603b\u5173\u6ce8\u6210\u5458\u3001\u5e8f\u5217\u548c\u8986\u76d6\u3002\u529f\u80fd\u6ce8\u91ca\u3001\u5206\u7c7b\u548c\u57fa\u4e8e\u6807\u8bb0\u7684\u8d28\u91cf\u8bc4\u4ef7\uff0c\u9700\u8981\u76f8\u5e94\u8bc1\u636e\u52a0\u5165\u6570\u636e\u5e93\u540e\u518d\u89e3\u91ca\u3002\u62ff\u5230\u4e00\u4e2a\u6c47\u603b\u76ee\u5f55\u65f6\uff0c\u53ef\u4ee5\u5148\u68c0\u67e5\u5b83\u5b9e\u9645\u5305\u542b\u54ea\u4e9b\u7ed3\u679c\u6587\u4ef6\uff0c\u518d\u636e\u6b64\u7ec4\u7ec7\u540e\u7eed\u5206\u6790\u3002<\/p>\n
\u5982\u4f55\u533a\u5206\u6df1\u5ea6\u3001\u8986\u76d6\u8303\u56f4\u548c\u6210\u5458\u6570\u91cf<\/h2>\n
\u672c\u4f8b\u8fd8\u53ef\u4ee5\u7528\u6765\u8bad\u7ec3\u4e09\u4e2a\u6307\u6807\u7684\u8bfb\u6cd5\u3002\u7532\u4e59\u4e24\u4e2a\u7247\u6bb5\u7684\u8986\u76d6\u68c0\u6d4b\u6bd4\u4f8b\u90fd\u662f\u4e00\uff0c\u56e0\u4e3a\u6bcf\u4e2a\u4f4d\u7f6e\u81f3\u5c11\u88ab\u4e00\u6761\u8bfb\u6bb5\u8986\u76d6\u3002\u5b83\u4eec\u7684\u5e73\u5747\u6df1\u5ea6\u5206\u522b\u662f\u4e00\u548c\u4e09\uff0c\u56e0\u4e3a\u4e59\u7684\u6bcf\u4e2a\u4f4d\u7f6e\u90fd\u6709\u4e09\u6761\u91cd\u53e0\u8bfb\u6bb5\u3002\u540c\u6837\u7684\u8986\u76d6\u8303\u56f4\u53ef\u4ee5\u5bf9\u5e94\u4e0d\u540c\u6df1\u5ea6\uff0c\u6240\u4ee5\u6c47\u603b\u8868\u4e2d\u7684\u4e24\u4e2a\u5b57\u6bb5\u5e94\u8be5\u540c\u65f6\u4fdd\u7559\u3002<\/p>\n
\u8ba1\u7b97\u603b\u91cf\u65f6\u8fd8\u8981\u4fdd\u7559\u957f\u5ea6\u6743\u91cd\u3002\u7532\u7247\u6bb5\u6709\u56db\u4e07\u4e94\u5343\u4e2a\u4f4d\u7f6e\uff0c\u6bcf\u4e2a\u4f4d\u7f6e\u6df1\u5ea6\u4e00\uff0c\u7d2f\u79ef\u6df1\u5ea6\u4e3a\u56db\u4e07\u4e94\u5343\u3002\u4e59\u7247\u6bb5\u6709\u4e00\u4e07\u4e94\u5343\u4e2a\u4f4d\u7f6e\uff0c\u6bcf\u4e2a\u4f4d\u7f6e\u6df1\u5ea6\u4e09\uff0c\u7d2f\u79ef\u6df1\u5ea6\u540c\u6837\u4e3a\u56db\u4e07\u4e94\u5343\u3002\u56e0\u6b64\u4e24\u4e2a\u7247\u6bb5\u867d\u7136\u5e73\u5747\u6df1\u5ea6\u4e0d\u540c\uff0c\u672c\u6b21\u6d88\u8017\u7684\u6bd4\u5bf9\u78b1\u57fa\u6570\u76f8\u540c\uff0c\u8fd9\u4e5f\u4e0e\u5404\u81ea\u56db\u767e\u4e94\u5341\u6761\u4e00\u767e\u78b1\u57fa\u8bfb\u6bb5\u76f8\u7b26\u3002<\/p>\n
\u8fd9\u7ec4\u5173\u7cfb\u53ef\u4ee5\u5f62\u6210\u4e00\u4e2a\u5f88\u5c0f\u7684\u9a8c\u7b97\u94fe\u3002\u5148\u7528\u7247\u6bb5\u957f\u5ea6\u4e58\u5e73\u5747\u6df1\u5ea6\uff0c\u5f97\u5230\u8986\u76d6\u7684\u78b1\u57fa\u603b\u91cf\uff0c\u518d\u4e0e\u6bd4\u5bf9\u8bb0\u5f55\u7684\u6709\u6548\u5bf9\u9f50\u957f\u5ea6\u76f8\u52a0\u7ed3\u679c\u6bd4\u8f83\u3002\u672c\u6b21\u91c7\u7528\u5b8c\u6574\u5339\u914d\uff0c\u8ba1\u7b97\u8fc7\u7a0b\u5341\u5206\u76f4\u63a5\u3002\u771f\u5b9e\u6570\u636e\u82e5\u5305\u542b\u8f6f\u526a\u5207\u3001\u7f3a\u53e3\u3001\u91cd\u590d\u6bd4\u5bf9\u53ca\u4e0d\u540c\u8fc7\u6ee4\u53e3\u5f84\uff0c\u9700\u8981\u6309\u7167\u5b9e\u9645\u4fdd\u7559\u7684\u5bf9\u9f50\u90e8\u5206\u91cd\u65b0\u5b9a\u4e49\u8fd9\u6761\u6838\u5bf9\u5173\u7cfb\u3002<\/p>\n
\u9879\u76ee\u89c4\u6a21\u6269\u5927\u540e\uff0c\u53ef\u4ee5\u7ed9\u6bcf\u4e2a\u539f\u59cb\u7247\u6bb5\u4fdd\u7559\u4e00\u884c\u57fa\u672c\u4fe1\u606f\uff0c\u8bb0\u5f55\u540d\u79f0\u3001\u957f\u5ea6\u3001\u5e8f\u5217\u6821\u9a8c\u503c\u548c\u6700\u7ec8\u7bb1\u540d\u79f0\u3002\u53e6\u5916\u5355\u72ec\u4fdd\u5b58\u5206\u5272\u6620\u5c04\u8868\uff0c\u7528\u7236\u7247\u6bb5\u540d\u79f0\u8fde\u63a5\u4e24\u4e2a\u5c42\u7ea7\u3002\u8fd9\u6837\u65e2\u80fd\u6309\u539f\u59cb\u7247\u6bb5\u7edf\u8ba1\u6700\u7ec8\u5e8f\u5217\uff0c\u4e5f\u80fd\u6309\u5206\u5272\u5355\u5143\u68c0\u67e5\u5c40\u90e8\u8986\u76d6\u5f02\u5e38\uff0c\u6210\u5458\u6570\u91cf\u53d8\u5316\u53d1\u751f\u5728\u54ea\u4e00\u5c42\u4f1a\u66f4\u52a0\u6e05\u695a\u3002<\/p>\n
\u81ea\u5df1\u7684\u6570\u636e\u9047\u5230\u540d\u79f0\u4e0d\u5339\u914d\u65f6\u600e\u6837\u6392\u67e5<\/h2>\n
\u7b2c\u4e00\u6b65\uff0c\u53d6\u51fa\u8f93\u5165\u5f52\u5c5e\u8868\u4e2d\u7684\u51e0\u4e2a\u540d\u79f0\uff0c\u548c\u6570\u636e\u5e93\u5b9e\u9645\u4fdd\u5b58\u7684\u540d\u79f0\u6bd4\u8f83\u3002\u5982\u679c\u8f93\u5165\u662f\u539f\u59cb\u7247\u6bb5\uff0c\u6570\u636e\u5e93\u9879\u76ee\u662f\u5e26\u5206\u5272\u540e\u7f00\u7684\u540d\u79f0\uff0c\u53ef\u4ee5\u6838\u5bf9\u662f\u5426\u9700\u8981\u7247\u6bb5\u6a21\u5f0f\u3002\u5982\u679c\u4e24\u8fb9\u7684\u524d\u7f00\u90fd\u5b8c\u5168\u4e0d\u540c\uff0c\u5219\u5e94\u7ee7\u7eed\u68c0\u67e5\u662f\u5426\u7528\u4e86\u53e6\u4e00\u4efd\u7ec4\u88c5\u6216\u53e6\u4e00\u7248\u91cd\u547d\u540d\u6587\u4ef6\u3002<\/p>\n
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