{"id":72728,"date":"2026-11-15T10:12:54","date_gmt":"2026-11-15T02:12:54","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72728"},"modified":"2026-11-15T10:12:54","modified_gmt":"2026-11-15T02:12:54","slug":"metawrap-shared-contig-dereplication-bin-membership","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/metawrap-shared-contig-dereplication-bin-membership\/","title":{"rendered":"metaWRAP \u7cbe\u4fee\u540e\u4e3a\u4ec0\u4e48\u5c11\u4e86\u4e00\u6761 contig\uff1f\u5b9e\u8dd1\u5171\u4eab\u7247\u6bb5\u7684\u4e09\u79cd\u5f52\u5c5e\u7ed3\u679c"},"content":{"rendered":"
\n

\u628a\u591a\u4e2a\u5206\u7bb1\u7ed3\u679c\u6574\u5408\u8d77\u6765\u4ee5\u540e\uff0c\u540c\u4e00\u6761\u7ec4\u88c5\u7247\u6bb5\u53ef\u80fd\u540c\u65f6\u51fa\u73b0\u5728\u4e24\u4e2a\u5019\u9009\u7bb1\u4e2d\u3002\u63a5\u4e0b\u6765\u5e94\u8be5\u628a\u5b83\u4fdd\u7559\u7ed9\u54ea\u4e2a\u7bb1\uff0c\u6216\u8005\u662f\u5426\u5e94\u5f53\u4ece\u4e24\u4e2a\u7bb1\u91cc\u90fd\u5220\u9664\uff0c\u4f1a\u76f4\u63a5\u5f71\u54cd\u6700\u7ec8\u5e8f\u5217\u96c6\u5408\u3002\u53ea\u770b\u8f93\u51fa\u76ee\u5f55\u91cc\u8fd8\u6709\u591a\u5c11\u4e2a\u6587\u4ef6\uff0c\u5f88\u96be\u53d1\u73b0\u8fd9\u79cd\u53d8\u5316\u3002<\/p>\n

\u672c\u6587\u56f4\u7ed5 metaWRAP \u7cbe\u4fee\u6d41\u7a0b\u4e2d\u7684\u5171\u4eab\u7247\u6bb5\u5904\u7406\u73af\u8282\u5c55\u5f00\u3002\u6211\u4eec\u5b89\u88c5\u72ec\u7acb\u7684 Python 2.7.15 \u73af\u5883\uff0c\u8fd0\u884c\u4f5c\u8005\u4ed3\u5e93\u672a\u7ecf\u4fee\u6539\u7684\u539f\u59cb\u811a\u672c\uff0c\u7528\u516d\u6761\u53ef\u9010\u5b57\u6838\u5bf9\u7684\u6559\u5b66\u5e8f\u5217\u5b8c\u6210\u4e09\u7ec4\u5bf9\u7167\u3002\u5b9e\u9a8c\u5b9e\u9645\u6539\u53d8\u4e86\u5171\u4eab\u7247\u6bb5\u7684\u53bb\u5411\uff0c\u4e5f\u6d4b\u91cf\u4e86\u6bcf\u7ec4\u8f93\u51fa\u7684\u6210\u5458\u3001\u957f\u5ea6\u3001\u91cd\u590d\u5f52\u5c5e\u548c\u4e22\u5931\u60c5\u51b5\u3002<\/p>\n

\u672c\u6b21\u5b8c\u6574\u6267\u884c\u7684\u662f\u5171\u4eab\u7247\u6bb5\u53bb\u91cd\u9636\u6bb5\u3002\u8f93\u5165\u5b8c\u6574\u5ea6\u548c\u6c61\u67d3\u5ea6\u662f\u4e13\u95e8\u8bbe\u8ba1\u7684\u6559\u5b66\u6570\u503c\uff0c\u7528\u6765\u63a7\u5236\u5019\u9009\u6392\u5e8f\u3002\u672c\u4f8b\u6ca1\u6709\u8fd0\u884c\u5b8c\u6574\u7684\u539f\u59cb\u8bfb\u6bb5\u5904\u7406\u3001\u7ec4\u88c5\u53ca CheckM \u8d28\u91cf\u8bc4\u4f30\uff0c\u4e0b\u9762\u7684\u7ed3\u679c\u7528\u4e8e\u89e3\u91ca\u6210\u5458\u9009\u62e9\u89c4\u5219\u3002<\/p>\n

\"\u7bb1\u6570\u6ca1\u53d8\uff0c\u5e8f\u5217\u4e3a\u4f55\u5c11\u4e86\u3002\u5b9e\u8dd1\u5171\u4eab\u7247\u6bb5\u7684\u4fdd\u7559\u3001\u5220\u9664\u4e0e\u5f52\u5c5e\u7ffb\u8f6c\u3002\u9ed8\u8ba4\u4fdd\u7559\uff0c6
\u5b9e\u8dd1\u5171\u4eab\u7247\u6bb5\u7684\u4fdd\u7559\u3001\u5220\u9664\u4e0e\u5f52\u5c5e\u7ffb\u8f6c<\/figcaption><\/figure>\n

\u5148\u5f04\u6e05\u695a\u7cbe\u4fee\u5728\u5904\u7406\u54ea\u4e00\u5c42\u95ee\u9898<\/h2>\n

\u540c\u4e00\u4efd\u7ec4\u88c5\u53ef\u4ee5\u4ea4\u7ed9\u4e0d\u540c\u5206\u7bb1\u5668\uff0c\u5f97\u5230\u591a\u5957\u5019\u9009\u57fa\u56e0\u7ec4\u3002\u5b83\u4eec\u53ef\u80fd\u5bf9\u5927\u90e8\u5206\u7247\u6bb5\u8fbe\u6210\u4e00\u81f4\uff0c\u4e5f\u53ef\u80fd\u628a\u8fb9\u754c\u7247\u6bb5\u653e\u8fdb\u4e0d\u540c\u7684\u7bb1\u3002\u6574\u5408\u6d41\u7a0b\u9700\u8981\u540c\u65f6\u5904\u7406\u5019\u9009\u4e4b\u95f4\u7684\u91cd\u53e0\u3001\u8d28\u91cf\u8bc4\u4ef7\u548c\u6700\u7ec8\u6210\u5458\u5f52\u5c5e\u3002<\/p>\n

metaWRAP \u63d0\u4f9b\u4ece\u8bfb\u6bb5\u5904\u7406\u5230\u5b8f\u57fa\u56e0\u7ec4\u5206\u6790\u7684\u4e00\u7ec4\u6a21\u5757\uff0c\u5176\u4e2d\u5206\u7bb1\u7cbe\u4fee\u6a21\u5757\u53ef\u4ee5\u63a5\u6536\u591a\u5957\u5019\u9009\u5206\u7bb1\uff0c\u7ed3\u5408\u8d28\u91cf\u7edf\u8ba1\u8fdb\u884c\u6574\u5408\u3002\u4f7f\u7528\u65f6\u5e94\u533a\u5206\u5019\u9009\u7248\u672c\u7684\u9009\u62e9\u4e0e\u67d0\u6761\u5171\u4eab\u7247\u6bb5\u7684\u5904\u7406\uff0c\u8fd9\u4e24\u4e2a\u52a8\u4f5c\u90fd\u4f1a\u6539\u53d8\u6700\u7ec8\u8f93\u51fa\u3002\u9879\u76ee\u4e3b\u9875<\/a><\/p>\n

\u672c\u6587\u805a\u7126\u540e\u4e00\u4e2a\u52a8\u4f5c\u3002\u8fdb\u5165\u8fd9\u4e2a\u9636\u6bb5\u4ee5\u540e\uff0c\u7a0b\u5e8f\u5df2\u7ecf\u62ff\u5230\u4e00\u7ec4\u5019\u9009\u7bb1\u548c\u5bf9\u5e94\u7edf\u8ba1\u8868\uff0c\u63a5\u4e0b\u6765\u6839\u636e\u7247\u6bb5\u540d\u79f0\u67e5\u627e\u91cd\u590d\u6210\u5458\uff0c\u518d\u51b3\u5b9a\u8f93\u51fa\u65f6\u4fdd\u7559\u54ea\u4efd\u5f52\u5c5e\u3002\u7406\u89e3\u8fd9\u4e2a\u5c40\u90e8\u6b65\u9aa4\uff0c\u80fd\u5e2e\u52a9\u8bfb\u8005\u89e3\u91ca\u5b8c\u6574\u6d41\u7a0b\u7ed3\u675f\u540e\u67d0\u6761\u7247\u6bb5\u4e3a\u4f55\u6d88\u5931\uff0c\u6216\u8005\u4e3a\u4f55\u53ea\u7559\u5728\u53e6\u4e00\u4e2a\u7bb1\u4e2d\u3002<\/p>\n

\u51c6\u5907\u8f93\u5165\u65f6\uff0c\u51e0\u5957\u5206\u7bb1\u5e94\u4fdd\u6301\u4e0e\u539f\u59cb\u7ec4\u88c5\u7684\u540d\u79f0\u5bf9\u5e94\u5173\u7cfb\u3002\u5982\u679c\u540c\u4e00\u6761\u7247\u6bb5\u5728\u4e0d\u540c\u5019\u9009\u76ee\u5f55\u91cc\u4f7f\u7528\u4e86\u4e0d\u540c\u540d\u79f0\uff0c\u5c31\u9700\u8981\u5148\u6062\u590d\u7edf\u4e00\u6620\u5c04\u3002\u5bf9\u4e8e\u6765\u6e90\u4e0d\u540c\u7684\u72ec\u7acb\u7ec4\u88c5\uff0c\u5373\u4f7f\u67d0\u4e9b\u5e8f\u5217\u76f8\u4f3c\uff0c\u4e5f\u9700\u8981\u5148\u660e\u786e\u5982\u4f55\u5efa\u7acb\u540c\u4e00\u6027\u7684\u8bc1\u636e\u548c\u5bf9\u5e94\u5173\u7cfb\u3002<\/p>\n

\u516d\u6761\u5e8f\u5217\u600e\u6837\u5f62\u6210\u4e03\u6761\u6210\u5458\u8bb0\u5f55<\/h2>\n

\u672c\u6b21\u9a71\u52a8\u4f7f\u7528\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u751f\u6210\u516d\u6761\u5e8f\u5217\uff0c\u6bcf\u6761\u90fd\u662f\u4e00\u5343\u78b1\u57fa\uff0c\u540d\u79f0\u5206\u522b\u4e3a\u4e09\u4e2a\u7532\u7c7b\u7247\u6bb5\u3001\u4e24\u4e2a\u4e59\u7c7b\u7247\u6bb5\u4ee5\u53ca\u4e00\u6761\u5171\u4eab\u7247\u6bb5\u3002\u5171\u4eab\u7247\u6bb5\u5728\u4e24\u4e2a\u8f93\u5165\u7bb1\u4e2d\u5177\u6709\u5b8c\u5168\u76f8\u540c\u7684\u540d\u79f0\u548c\u5185\u5bb9\u3002<\/p>\n

\u7532\u7bb1\u5305\u542b\u56db\u6761\u5e8f\u5217\uff0c\u603b\u957f\u5ea6\u56db\u5343\u78b1\u57fa\u3002\u4e59\u7bb1\u5305\u542b\u4e09\u6761\u5e8f\u5217\uff0c\u603b\u957f\u5ea6\u4e09\u5343\u78b1\u57fa\u3002\u5982\u679c\u628a\u4e24\u4e2a\u6587\u4ef6\u7684\u8bb0\u5f55\u6570\u76f4\u63a5\u76f8\u52a0\uff0c\u4f1a\u5f97\u5230\u4e03\u6761\u8bb0\u5f55\u3002\u5982\u679c\u6309\u540d\u79f0\u548c\u5e8f\u5217\u5185\u5bb9\u6838\u5bf9\u5e76\u96c6\uff0c\u5219\u53ea\u6709\u516d\u6761\u552f\u4e00\u7247\u6bb5\uff0c\u603b\u957f\u5ea6\u516d\u5343\u78b1\u57fa\u3002<\/p>\n

\n\n\n\n\n\n\n
\u8f93\u5165\u7bb1<\/th>\n\u7247\u6bb5\u540d\u79f0<\/th>\n\u6587\u4ef6\u5185\u603b\u957f\u5ea6<\/th>\n\u6559\u5b66\u5b8c\u6574\u5ea6<\/th>\n\u6559\u5b66\u6c61\u67d3\u5ea6<\/th>\n<\/tr>\n<\/thead>\n
Alpha<\/td>\nA1\u3001A2\u3001A3\u3001shared<\/td>\n4000 bp<\/td>\n92<\/td>\n2<\/td>\n<\/tr>\n
Beta<\/td>\nB1\u3001B2\u3001shared<\/td>\n3000 bp<\/td>\n90<\/td>\n1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u8fd9\u4e2a\u7b80\u5355\u8f93\u5165\u63d0\u4f9b\u4e86\u4e24\u4e2a\u6838\u5bf9\u57fa\u51c6\u3002\u7b2c\u4e00\uff0c\u5408\u7406\u7684\u53bb\u91cd\u8f93\u51fa\u53ef\u4ee5\u4fdd\u7559\u516d\u6761\u552f\u4e00\u7247\u6bb5\uff0c\u540c\u65f6\u6d88\u9664\u91cd\u590d\u5f52\u5c5e\u3002\u7b2c\u4e8c\uff0c\u5982\u679c\u9009\u62e9\u5c06\u6b67\u4e49\u6210\u5458\u5168\u90e8\u5220\u9664\uff0c\u8f93\u51fa\u4f1a\u5c11\u6389\u5171\u4eab\u7247\u6bb5\uff0c\u552f\u4e00\u5e8f\u5217\u603b\u957f\u5ea6\u964d\u5230\u4e94\u5343\u78b1\u57fa\u3002\u4e24\u79cd\u7ed3\u679c\u90fd\u80fd\u7531\u5b9e\u9645\u6210\u5458\u5217\u8868\u76f4\u63a5\u8ba1\u7b97\u3002<\/p>\n

\u6559\u5b66\u5b8c\u6574\u5ea6\u548c\u6c61\u67d3\u5ea6\u53ea\u8d1f\u8d23\u89e6\u53d1\u9884\u671f\u7684\u6392\u5e8f\u5dee\u5f02\u3002\u8fd9\u4e9b\u77ed\u968f\u673a\u5e8f\u5217\u6ca1\u6709\u88ab\u8d4b\u4e88\u771f\u5b9e\u57fa\u56e0\u7ec4\u8d28\u91cf\u542b\u4e49\uff0c\u4e5f\u4e0d\u80fd\u636e\u6b64\u5224\u65ad\u54ea\u4e00\u4e2a\u7bb1\u5728\u751f\u7269\u5b66\u4e0a\u66f4\u6b63\u786e\u3002\u590d\u73b0\u65f6\u4fdd\u7559\u8fd9\u4e00\u8f93\u5165\u8bf4\u660e\uff0c\u624d\u80fd\u628a\u7b97\u6cd5\u884c\u4e3a\u548c\u8d28\u91cf\u6d4b\u91cf\u533a\u5206\u6e05\u695a\u3002<\/p>\n

\u539f\u59cb\u811a\u672c\u7528\u4ec0\u4e48\u6570\u503c\u51b3\u5b9a\u5f52\u5c5e<\/h2>\n

\u672c\u6b21\u56fa\u5b9a\u4f5c\u8005\u4ed3\u5e93\u63d0\u4ea4 08d8379d0193b0e29785b256b00602f4fdf4b601<\/code>\uff0c\u8fd0\u884c\u5176\u4e2d\u7684 dereplicate_contigs_in_bins.py<\/code>\u3002\u811a\u672c\u6821\u9a8c\u503c\u8bb0\u5f55\u5728\u4e0b\u8f7d\u5305\u4e2d\uff0c\u65b9\u4fbf\u786e\u8ba4\u8bfb\u53d6\u7684\u662f\u540c\u4e00\u4e2a\u7248\u672c\u3002<\/p>\n

\u5728\u8be5\u811a\u672c\u4e2d\uff0c\u6bcf\u4e2a\u7bb1\u7684\u8bc4\u5206\u7531\u5b8c\u6574\u5ea6\u3001\u6c61\u67d3\u5ea6\u548c\u4e00\u4e2a\u5f88\u5c0f\u7684\u8fde\u7eed\u6027\u9879\u7ec4\u6210\u3002\u5b9e\u9645\u8f93\u5165\u7edf\u8ba1\u8868\u7684\u7b2c\u516d\u5217\u662f\u957f\u5ea6\u6307\u6807\uff0c\u56e0\u6b64\u4f7f\u7528\u65f6\u8fd8\u5e94\u6838\u5bf9\u6587\u4ef6\u5217\u987a\u5e8f\u3002\u672c\u6b21\u8fd0\u884c\u7684\u539f\u59cb\u811a\u672c<\/a><\/p>\n

score = completeness - 5 * contamination + 1e-10 * N50\r\n\r\nAlpha = 92 - 5 * 2 + 1e-10 * 1000 = 82.0000001\r\nBeta  = 90 - 5 * 1 + 1e-10 * 1000 = 85.0000001\r\n<\/code><\/pre>\n
\u7532\u7684\u5b8c\u6574\u5ea6\u8f93\u5165\u66f4\u9ad8\uff0c\u4f46\u4e59\u7684\u6c61\u67d3\u5ea6\u8f93\u5165\u66f4\u4f4e\u3002\u6309\u7167\u672c\u6b21\u8bc4\u5206\u516c\u5f0f\uff0c\u4e59\u7684\u603b\u5206\u9ad8\u51fa\u4e09\u5206\uff0c\u56e0\u6b64\u5171\u4eab\u7247\u6bb5\u4f1a\u7559\u5728\u4e59\u7bb1\u3002\u8fd9\u4e2a\u7ed3\u679c\u8bf4\u660e\uff0c\u5728\u89e3\u91ca\u6210\u5458\u9009\u62e9\u65f6\uff0c\u5e94\u628a\u5b8c\u6574\u8bc4\u5206\u5217\u51fa\u6765\uff0c\u5355\u72ec\u6bd4\u8f83\u5b8c\u6574\u5ea6\u5f88\u5bb9\u6613\u8bef\u5224\u7a0b\u5e8f\u884c\u4e3a\u3002<\/p>\n
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\u811a\u672c\u9488\u5bf9\u91cd\u590d\u7684\u7247\u6bb5\u540d\u79f0\u5904\u7406\u5f52\u5c5e\u3002\u5b83\u672c\u8eab\u6ca1\u6709\u5728\u8fd9\u4e2a\u6b65\u9aa4\u91cd\u65b0\u8fdb\u884c\u5e8f\u5217\u6bd4\u5bf9\uff0c\u4e5f\u6ca1\u6709\u9010\u4e00\u6d4b\u91cf\u5171\u4eab\u7247\u6bb5\u4e0e\u5404\u4e2a\u7bb1\u7684\u751f\u7269\u5b66\u4e00\u81f4\u6027\u3002\u56e0\u6b64\uff0c\u5728\u5b83\u524d\u9762\u4fdd\u6301\u6b63\u786e\u7684\u540d\u79f0\u4e0e\u5e8f\u5217\u6620\u5c04\uff0c\u662f\u540e\u7eed\u89e3\u91ca\u6210\u7acb\u7684\u6761\u4ef6\u3002<\/p>\n
\u7b2c\u4e00\u7ec4\u5b9e\u8dd1\uff0c\u4fdd\u7559\u7ed9\u5206\u6570\u66f4\u9ad8\u7684\u7bb1<\/h2>\n
\u4e0b\u8f7d\u5305\u4e2d\u7684\u9a71\u52a8\u5148\u751f\u6210\u8f93\u5165\u5e8f\u5217\u548c\u7edf\u8ba1\u8868\uff0c\u7136\u540e\u901a\u8fc7 Python 2.7 \u8c03\u7528\u539f\u59cb\u7a0b\u5e8f\u3002\u4e0b\u9762\u5c55\u793a\u5b9e\u9645\u4f7f\u7528\u7684\u8c03\u7528\u5f62\u5f0f\uff0c\u7edf\u8ba1\u6587\u4ef6\u548c\u8f93\u5165\u76ee\u5f55\u5747\u7531\u9a71\u52a8\u521b\u5efa\uff0c\u8f93\u51fa\u653e\u5165\u72ec\u7acb\u76ee\u5f55\u3002<\/p>\n
python2.7 dereplicate_contigs_in_bins.py \\\r\n  best_score.stats input best_score\r\n<\/code><\/pre>\n
\u8f93\u51fa\u7532\u7bb1\u53ea\u5269\u4e09\u4e2a\u72ec\u6709\u7247\u6bb5\uff0c\u603b\u957f\u5ea6\u4e09\u5343\u78b1\u57fa\u3002\u4e59\u7bb1\u4fdd\u7559\u4e24\u4e2a\u72ec\u6709\u7247\u6bb5\u548c\u5171\u4eab\u7247\u6bb5\uff0c\u603b\u957f\u5ea6\u4e5f\u662f\u4e09\u5343\u78b1\u57fa\u3002\u6838\u5bf9\u4e24\u4e2a\u8f93\u51fa\u6587\u4ef6\u540e\uff0c\u5171\u5f97\u5230\u516d\u6761\u552f\u4e00\u7247\u6bb5\uff0c\u6ca1\u6709\u8de8\u7bb1\u91cd\u590d\uff0c\u4e5f\u6ca1\u6709\u4efb\u4f55\u8f93\u5165\u7247\u6bb5\u4ece\u5e76\u96c6\u4e2d\u4e22\u5931\u3002<\/p>\n
\u8fd9\u7ec4\u7ed3\u679c\u53ef\u4ee5\u56de\u7b54\u4e00\u4e2a\u7ecf\u5e38\u51fa\u73b0\u7684\u7591\u95ee\u3002\u7532\u7bb1\u53d8\u77ed\u4e86\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u6574\u5957\u7ed3\u679c\u635f\u5931\u4e86\u76f8\u540c\u957f\u5ea6\u7684\u552f\u4e00\u5e8f\u5217\u3002\u5171\u4eab\u7247\u6bb5\u4ecd\u7136\u4fdd\u5b58\u5728\u4e59\u7bb1\uff0c\u96c6\u5408\u5c42\u9762\u7684\u5e8f\u5217\u603b\u91cf\u4fdd\u6301\u516d\u5343\u78b1\u57fa\uff0c\u53ea\u662f\u540c\u4e00\u6761\u7247\u6bb5\u7684\u91cd\u590d\u5f52\u5c5e\u88ab\u6d88\u9664\u4e86\u3002<\/p>\n
\u56e0\u6b64\uff0c\u62a5\u544a\u7cbe\u4fee\u524d\u540e\u7684\u53d8\u5316\u65f6\uff0c\u53ef\u4ee5\u540c\u65f6\u7ed9\u51fa\u6bcf\u4e2a\u7bb1\u7684\u957f\u5ea6\u53d8\u5316\u548c\u6574\u4e2a\u96c6\u5408\u7684\u552f\u4e00\u5e8f\u5217\u53d8\u5316\u3002\u524d\u8005\u53cd\u6620\u7bb1\u7684\u6210\u5458\u8c03\u6574\uff0c\u540e\u8005\u53cd\u6620\u6700\u7ec8\u4fdd\u7559\u4e86\u591a\u5c11\u5185\u5bb9\u3002\u628a\u8fd9\u4e24\u4e2a\u5c42\u6b21\u5206\u5f00\uff0c\u624d\u80fd\u89e3\u91ca\u540c\u4e00\u9879\u5904\u7406\u4e3a\u4f55\u65e2\u8ba9\u67d0\u4e2a\u6587\u4ef6\u7f29\u77ed\uff0c\u53c8\u6ca1\u6709\u9020\u6210\u96c6\u5408\u5e8f\u5217\u4e22\u5931\u3002<\/p>\n
\u7b2c\u4e8c\u7ec4\u5b9e\u8dd1\uff0c\u4ece\u6240\u6709\u7bb1\u4e2d\u5220\u9664\u5171\u4eab\u6210\u5458<\/h2>\n
\u7b2c\u4e8c\u7ec4\u4fdd\u6301\u5e8f\u5217\u548c\u8bc4\u5206\u8868\u4e0d\u53d8\uff0c\u53ea\u7ed9\u539f\u59cb\u811a\u672c\u589e\u52a0\u5220\u9664\u6a21\u5f0f\u53c2\u6570\u3002\u8be5\u9636\u6bb5\u628a\u91cd\u590d\u51fa\u73b0\u7684\u6210\u5458\u6807\u8bb0\u4e3a\u4e0d\u4fdd\u7559\uff0c\u968f\u540e\u5199\u51fa\u6bcf\u4e2a\u7bb1\u5269\u4f59\u7684\u5e8f\u5217\u3002<\/p>\n
python2.7 dereplicate_contigs_in_bins.py \\\r\n  remove_both.stats input remove_both remove\r\n<\/code><\/pre>\n
\u5b9e\u9645\u8f93\u51fa\u4e2d\uff0c\u7532\u7bb1\u4fdd\u7559\u4e09\u4e2a\u7247\u6bb5\uff0c\u4e59\u7bb1\u53ea\u5269\u4e24\u4e2a\u3002\u4e24\u8005\u603b\u5171\u4e94\u6761\u552f\u4e00\u7247\u6bb5\uff0c\u957f\u5ea6\u4e94\u5343\u78b1\u57fa\u3002\u5171\u4eab\u7247\u6bb5\u51fa\u73b0\u5728\u8f93\u5165\u5e76\u96c6\u4e0e\u8f93\u51fa\u5e76\u96c6\u7684\u5dee\u96c6\u4e2d\uff0c\u7a0b\u5e8f\u4fdd\u5b58\u7684\u4e22\u5931\u540d\u5355\u4e5f\u660e\u786e\u8bb0\u5f55\u4e86\u5b83\u3002<\/p>\n
\u5b8c\u6574\u7cbe\u4fee\u6a21\u5757\u4e2d\uff0c\u5b98\u65b9\u63d0\u4f9b\u5220\u9664\u6b67\u4e49\u7247\u6bb5\u7684\u9009\u9879\uff0c\u5c06\u8fd9\u7c7b\u5904\u7406\u63a5\u5165\u6d41\u7a0b\u3002\u672c\u6b21\u9a8c\u8bc1\u7684\u662f\u5b83\u8c03\u7528\u7684\u6210\u5458\u53bb\u91cd\u884c\u4e3a\uff0c\u9009\u9879\u542b\u4e49\u53ef\u4ee5\u5728\u56fa\u5b9a\u7248\u672c\u7684\u6a21\u5757\u5e2e\u52a9\u4e0e\u6e90\u7801\u4e2d\u67e5\u9605\u3002\u5206\u7bb1\u7cbe\u4fee\u6a21\u5757\u8bf4\u660e\u4e0e\u5b9e\u73b0<\/a><\/p>\n
\u8fd9\u91cc\u51cf\u5c11\u4e86\u4e00\u5343\u78b1\u57fa\uff0c\u7ea6\u5360\u8f93\u5165\u552f\u4e00\u5e8f\u5217\u603b\u91cf\u7684\u516d\u5206\u4e4b\u4e00\u3002\u5982\u679c\u53ea\u6bd4\u8f83\u6587\u4ef6\u6570\u91cf\uff0c\u4e24\u7ec4\u7ed3\u679c\u59cb\u7ec8\u90fd\u662f\u4e24\u4e2a\u7bb1\uff0c\u5b8c\u5168\u770b\u4e0d\u51fa\u8fd9\u4e00\u5dee\u5f02\u3002\u5373\u4f7f\u5728\u771f\u5b9e\u9879\u76ee\u4e2d\u635f\u5931\u6bd4\u4f8b\u8f83\u5c0f\uff0c\u4e5f\u503c\u5f97\u4fdd\u7559\u5177\u4f53\u4e22\u5931\u540d\u5355\uff0c\u68c0\u67e5\u8fd9\u4e9b\u7247\u6bb5\u662f\u5426\u5e26\u6709\u9700\u8981\u89e3\u91ca\u7684\u529f\u80fd\u6216\u6807\u8bb0\u3002<\/p>\n
\u672c\u4f8b\u53ea\u6d4b\u91cf\u4e86\u6210\u5458\u5220\u9664\u540e\u7684\u5e8f\u5217\u53d8\u5316\u3002\u5220\u9664\u662f\u5426\u6709\u52a9\u4e8e\u6539\u5584\u5b9e\u9645\u6c61\u67d3\u4f30\u8ba1\uff0c\u53d6\u51b3\u4e8e\u7247\u6bb5\u5185\u5bb9\u53ca\u8d28\u91cf\u8bc4\u4f30\u8bc1\u636e\uff0c\u9700\u8981\u5bf9\u6700\u7ec8\u5e8f\u5217\u91cd\u65b0\u8bc4\u4ef7\u540e\u518d\u4f5c\u5224\u65ad\u3002<\/p>\n
\u7b2c\u4e09\u7ec4\u5b9e\u8dd1\uff0c\u53ea\u6539\u53d8\u8bc4\u5206\u5c31\u80fd\u7ffb\u8f6c\u5f52\u5c5e<\/h2>\n
\u7b2c\u4e09\u7ec4\u4ecd\u7136\u4f7f\u7528\u540c\u6837\u516d\u6761\u5e8f\u5217\uff0c\u4fdd\u7559\u9ed8\u8ba4\u5f52\u5c5e\u6a21\u5f0f\u3002\u6211\u4eec\u628a\u7532\u7bb1\u7684\u6559\u5b66\u5b8c\u6574\u5ea6\u6539\u4e3a\u4e5d\u5341\u516b\u3001\u6c61\u67d3\u5ea6\u6539\u4e3a\u4e00\uff0c\u4e59\u7bb1\u7684\u4e24\u4e2a\u6570\u503c\u4fdd\u6301\u4e0d\u53d8\u3002<\/p>\n
\u7532\u7684\u8bc4\u5206\u5347\u5230\u4e5d\u5341\u4e09\u70b9\u96f6\u96f6\u96f6\u96f6\u96f6\u96f6\u4e00\uff0c\u8d85\u8fc7\u4e59\u7684\u516b\u5341\u4e94\u70b9\u96f6\u96f6\u96f6\u96f6\u96f6\u96f6\u4e00\u3002\u5b9e\u9645\u7ed3\u679c\u4e2d\uff0c\u5171\u4eab\u7247\u6bb5\u8f6c\u800c\u7559\u5728\u7532\u7bb1\u3002\u7532\u7bb1\u6062\u590d\u56db\u5343\u78b1\u57fa\uff0c\u4e59\u7bb1\u7f29\u77ed\u5230\u4e24\u5343\u78b1\u57fa\uff0c\u96c6\u5408\u4ecd\u7136\u4fdd\u7559\u516d\u6761\u552f\u4e00\u7247\u6bb5\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u5b9e\u8dd1\u6761\u4ef6<\/th>\nshared \u6700\u7ec8\u53bb\u5411<\/th>\nAlpha \u957f\u5ea6<\/th>\nBeta \u957f\u5ea6<\/th>\n\u552f\u4e00\u7247\u6bb5\u603b\u6570<\/th>\n\u4e22\u5931\u7247\u6bb5<\/th>\n<\/tr>\n<\/thead>\n
\u521d\u59cb\u8bc4\u5206\uff0c\u9ed8\u8ba4\u4fdd\u7559<\/td>\nBeta<\/td>\n3000 bp<\/td>\n3000 bp<\/td>\n6<\/td>\n\u65e0<\/td>\n<\/tr>\n
\u521d\u59cb\u8bc4\u5206\uff0c\u5168\u90e8\u5220\u9664<\/td>\n\u65e0<\/td>\n3000 bp<\/td>\n2000 bp<\/td>\n5<\/td>\nshared<\/td>\n<\/tr>\n
\u63d0\u9ad8 Alpha \u8bc4\u5206<\/td>\nAlpha<\/td>\n4000 bp<\/td>\n2000 bp<\/td>\n6<\/td>\n\u65e0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8fd9\u9879\u5bf9\u7167\u628a\u5e8f\u5217\u5185\u5bb9\u56fa\u5b9a\u4e0b\u6765\uff0c\u53ea\u6539\u53d8\u8f93\u5165\u8d28\u91cf\u6570\u5b57\uff0c\u5c31\u80fd\u89c2\u5bdf\u5230\u5f52\u5c5e\u7ffb\u8f6c\u3002\u5b83\u8bf4\u660e\u8d28\u91cf\u7edf\u8ba1\u8868\u5c5e\u4e8e\u51b3\u5b9a\u7ed3\u679c\u7684\u5206\u6790\u8f93\u5165\uff0c\u5e94\u5f53\u548c\u5bf9\u5e94\u7684\u5019\u9009\u5e8f\u5217\u4e00\u8d77\u4fdd\u5b58\u3002\u91cd\u65b0\u8fd0\u884c\u8d28\u91cf\u8bc4\u4f30\u6216\u8005\u6539\u7528\u53e6\u4e00\u7248\u5019\u9009\u6587\u4ef6\u540e\uff0c\u90fd\u9700\u8981\u786e\u8ba4\u7edf\u8ba1\u8868\u4e0e\u5e8f\u5217\u4ecd\u7136\u914d\u5957\u3002<\/p>\n
\u5728\u771f\u5b9e\u6570\u636e\u4e2d\uff0c\u5019\u9009\u7bb1\u7684\u8d28\u91cf\u53d8\u5316\u901a\u5e38\u4f34\u968f\u5e8f\u5217\u5185\u5bb9\u53d8\u5316\uff0c\u60c5\u51b5\u4f1a\u66f4\u52a0\u590d\u6742\u3002\u672c\u4f8b\u6545\u610f\u628a\u4e8c\u8005\u5206\u5f00\uff0c\u4fbf\u4e8e\u89c2\u5bdf\u7a0b\u5e8f\u5bf9\u8bc4\u5206\u7684\u54cd\u5e94\u3002\u8bfb\u8005\u590d\u73b0\u65f6\u4e5f\u53ef\u4ee5\u91c7\u7528\u76f8\u540c\u601d\u8def\uff0c\u6bcf\u6b21\u53ea\u6539\u53d8\u4e00\u4e2a\u660e\u786e\u56e0\u7d20\uff0c\u518d\u6bd4\u8f83\u5b9e\u9645\u6210\u5458\u5dee\u5f02\u3002<\/p>\n
\u7ed9\u81ea\u5df1\u7684\u7ed3\u679c\u505a\u4e00\u6b21\u6210\u5458\u5ba1\u8ba1<\/h2>\n
\u6700\u57fa\u7840\u7684\u68c0\u67e5\u662f\u5efa\u7acb\u4e00\u5f20\u7247\u6bb5\u5230\u7bb1\u7684\u5bf9\u5e94\u8868\u3002\u6bcf\u8bfb\u5230\u4e00\u6761\u5e8f\u5217\uff0c\u5c31\u8bb0\u5f55\u5b83\u7684\u540d\u79f0\u3001\u5e8f\u5217\u957f\u5ea6\u548c\u6240\u5728\u7bb1\u3002\u5982\u679c\u540c\u4e00\u4e2a\u540d\u79f0\u51fa\u73b0\u591a\u6b21\uff0c\u8fd8\u5e94\u6838\u5bf9\u5185\u5bb9\u662f\u5426\u4e00\u81f4\uff0c\u907f\u514d\u628a\u547d\u540d\u51b2\u7a81\u8bef\u5f53\u4f5c\u771f\u5b9e\u5171\u4eab\u3002<\/p>\n
\u7136\u540e\u5206\u522b\u8ba1\u7b97\u8f93\u5165\u548c\u8f93\u51fa\u7684\u552f\u4e00\u7247\u6bb5\u96c6\u5408\u3002\u8f93\u51fa\u4e2d\u51fa\u73b0\u6b21\u6570\u5927\u4e8e\u4e00\u7684\u6210\u5458\u662f\u6b8b\u7559\u91cd\u590d\u5f52\u5c5e\uff0c\u8f93\u5165\u4e2d\u5b58\u5728\u800c\u8f93\u51fa\u4e2d\u6ca1\u6709\u7684\u6210\u5458\u662f\u4e22\u5931\u5bf9\u8c61\uff0c\u8f93\u51fa\u65b0\u589e\u800c\u8f93\u5165\u4e0d\u5b58\u5728\u7684\u540d\u79f0\u5219\u9700\u8981\u56de\u67e5\u6765\u6e90\u3002\u4e0b\u8f7d\u5305\u4e2d\u7684\u9a71\u52a8\u5b9e\u9645\u5b8c\u6210\u4e86\u8fd9\u4e9b\u68c0\u67e5\u3002<\/p>\n
\u672c\u6b21\u8fd8\u5bf9\u6bcf\u6761\u4fdd\u7559\u5e8f\u5217\u505a\u4e86\u9010\u5b57\u6bd4\u8f83\uff0c\u786e\u4fdd\u8f93\u51fa\u5185\u5bb9\u4e0e\u8f93\u5165\u4e00\u81f4\u3002\u8fd9\u4e00\u6b65\u80fd\u786e\u8ba4\u5b9e\u9a8c\u89c2\u5bdf\u5230\u7684\u662f\u6210\u5458\u4fdd\u7559\u548c\u5220\u9664\uff0c\u800c\u6ca1\u6709\u540c\u65f6\u53d1\u751f\u65e0\u6cd5\u89e3\u91ca\u7684\u5e8f\u5217\u4fee\u6539\u3002\u5bf9\u4e8e\u89c4\u6a21\u66f4\u5927\u7684\u6587\u4ef6\uff0c\u53ef\u4ee5\u5728\u4e00\u81f4\u7684\u5e8f\u5217\u6807\u51c6\u5316\u89c4\u5219\u4e0b\u8bb0\u5f55\u6821\u9a8c\u503c\u3002<\/p>\n
\u5ba1\u8ba1\u8868\u6700\u597d\u5305\u542b\u6539\u53d8\u524d\u540e\u7684\u7bb1\u540d\u79f0\u548c\u53d8\u5316\u539f\u56e0\u3002\u4f8b\u5982\u5171\u4eab\u7247\u6bb5\u539f\u6765\u540c\u65f6\u5c5e\u4e8e\u7532\u4e59\u4e24\u7bb1\uff0c\u9ed8\u8ba4\u8fd0\u884c\u540e\u53ea\u5c5e\u4e8e\u4e59\uff0c\u539f\u56e0\u662f\u4e59\u7684\u8f93\u5165\u8bc4\u5206\u66f4\u9ad8\u3002\u8fd9\u6837\u7684\u8bb0\u5f55\u6bd4\u53ea\u4fdd\u5b58\u6700\u7ec8\u76ee\u5f55\u66f4\u5bb9\u6613\u652f\u6301\u590d\u67e5\uff0c\u4e5f\u4fbf\u4e8e\u5411\u5408\u4f5c\u8005\u89e3\u91ca\u540e\u7eed\u529f\u80fd\u7edf\u8ba1\u4e3a\u4ec0\u4e48\u53d1\u751f\u53d8\u5316\u3002<\/p>\n
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