{"id":72753,"date":"2026-11-15T10:34:48","date_gmt":"2026-11-15T02:34:48","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72753"},"modified":"2026-11-15T10:34:48","modified_gmt":"2026-11-15T02:34:48","slug":"kaiju-protein-search-lca-reference-database-controls","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/kaiju-protein-search-lca-reference-database-controls\/","title":{"rendered":"Kaiju \u4e3a\u4ec0\u4e48\u628a\u540c\u4e00\u6761\u8bfb\u6bb5\u5206\u5230\u5c5e\u6216\u79cd\uff1f\u5b9e\u8dd1\u7ffb\u8bd1\u641c\u7d22\u3001\u5339\u914d\u95e8\u69db\u548c\u53c2\u8003\u5e93\u53d8\u5316"},"content":{"rendered":"
\n

\u5b8f\u57fa\u56e0\u7ec4\u5206\u7c7b\u8868\u91cc\uff0c\u4e00\u4e2a\u5e38\u89c1\u73b0\u8c61\u662f\u67d0\u6761\u8bfb\u6bb5\u5728\u4e00\u4e2a\u53c2\u8003\u5e93\u4e2d\u53ea\u80fd\u5206\u5230\u5c5e\uff0c\u6362\u4e00\u4e2a\u5e93\u4ee5\u540e\u5374\u80fd\u5206\u5230\u79cd\u3002\u770b\u5230\u5206\u7c7b\u5c42\u7ea7\u53d8\u7ec6\uff0c\u9700\u8981\u7ee7\u7eed\u68c0\u67e5\u5339\u914d\u8bc1\u636e\u548c\u5019\u9009\u96c6\u5408\uff0c\u624d\u80fd\u5224\u65ad\u7ed3\u679c\u5e94\u8be5\u600e\u6837\u89e3\u91ca\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u7f16\u8bd1 Kaiju 1.10.4\uff0c\u6784\u9020\u4e24\u4e2a\u86cb\u767d\u53c2\u8003\u3001\u516b\u6761\u6838\u9178\u8bfb\u6bb5\u548c\u4e00\u68f5\u6559\u5b66\u5206\u7c7b\u6811\uff0c\u5b8c\u6210\u5efa\u7d22\u5f15\u3001\u516d\u7ec4\u5206\u7c7b\u53ca\u5c5e\u79cd\u5c42\u7ea7\u6c47\u603b\u3002\u6240\u6709\u5e8f\u5217\u548c\u5206\u7c7b\u540d\u79f0\u90fd\u662f\u6559\u5b66\u8bbe\u8ba1\uff0c\u76ee\u7684\u662f\u628a\u7ffb\u8bd1\u641c\u7d22\u3001\u5171\u4eab\u5339\u914d\u548c\u53c2\u6570\u53d8\u5316\u653e\u5230\u53ef\u4ee5\u9010\u6761\u6838\u5bf9\u7684\u4f8b\u5b50\u4e2d\u3002<\/p>\n

\"\u5206\u5230\u79cd\uff0c\u5c31\u4e00\u5b9a\u66f4\u51c6\u786e\u5417\u3002Kaiju
Kaiju \u516d\u7ec4\u5b9e\u8dd1\u6838\u5bf9\u7ffb\u8bd1\u4e0e\u5171\u4eab\u5339\u914d<\/figcaption><\/figure>\n

\u86cb\u767d\u5c42\u9762\u7684\u641c\u7d22\u80fd\u89e3\u51b3\u4ec0\u4e48\u95ee\u9898<\/h2>\n

Kaiju \u4f7f\u7528\u86cb\u767d\u53c2\u8003\u5e93\u5bf9\u5b8f\u57fa\u56e0\u7ec4\u8bfb\u6bb5\u8fdb\u884c\u5206\u7c7b\u3002\u6838\u9178\u8bfb\u6bb5\u7ecf\u8fc7\u7ffb\u8bd1\u540e\uff0c\u53ef\u4ee5\u4f9d\u636e\u6c28\u57fa\u9178\u5339\u914d\u83b7\u5f97\u5206\u7c7b\u652f\u6301\u3002\u5bf9\u4e8e\u7f16\u7801\u76f8\u540c\u6c28\u57fa\u9178\u3001\u4f46\u4f7f\u7528\u4e0d\u540c\u5bc6\u7801\u5b50\u7684\u6838\u9178\u7247\u6bb5\uff0c\u8fd9\u79cd\u641c\u7d22\u80fd\u591f\u4fdd\u7559\u6838\u9178\u5b57\u9762\u5dee\u5f02\u80cc\u540e\u7684\u5173\u8054\u3002Kaiju \u5b98\u65b9\u8bf4\u660e<\/a><\/p>\n

\u4e0e\u6b64\u540c\u65f6\uff0c\u86cb\u767d\u5e8f\u5217\u5728\u4e0d\u540c\u751f\u7269\u4e4b\u95f4\u53ef\u80fd\u9ad8\u5ea6\u4fdd\u5b88\u3002\u4e00\u6bb5\u6c28\u57fa\u9178\u5339\u914d\u8db3\u4ee5\u652f\u6301\u8f83\u9ad8\u5c42\u7ea7\u7684\u5206\u7c7b\uff0c\u5374\u672a\u5fc5\u80fd\u591f\u533a\u5206\u8fd1\u7f18\u7269\u79cd\u3002\u8bc4\u4ef7\u7ed3\u679c\u65f6\uff0c\u9700\u8981\u540c\u65f6\u67e5\u770b\u5339\u914d\u957f\u5ea6\u3001\u5019\u9009\u5206\u7c7b\u7f16\u53f7\u53ca\u5b83\u4eec\u5728\u5206\u7c7b\u6811\u4e2d\u7684\u5173\u7cfb\u3002<\/p>\n

\u5b9e\u9645\u9879\u76ee\u8fd8\u5e94\u5148\u660e\u786e\u95ee\u9898\u3002\u5e0c\u671b\u4e86\u89e3\u5e7f\u6cdb\u7684\u5206\u7c7b\u7ec4\u6210\uff0c\u53ef\u4ee5\u5173\u6ce8\u5408\u9002\u5c42\u7ea7\u7684\u6c47\u603b\u548c\u672a\u5206\u7c7b\u6bd4\u4f8b\u3002\u5e0c\u671b\u786e\u8ba4\u67d0\u4e2a\u8fd1\u7f18\u79cd\uff0c\u5219\u5e94\u8fdb\u4e00\u6b65\u68c0\u67e5\u591a\u4e2a\u533a\u57df\u7684\u652f\u6301\u3001\u53c2\u8003\u4e4b\u95f4\u7684\u533a\u5206\u80fd\u529b\u548c\u5176\u4ed6\u8bc1\u636e\u3002\u672c\u6b21\u5b9e\u9a8c\u4f1a\u5c55\u793a\uff0c\u5355\u6761\u5171\u4eab\u7247\u6bb5\u7684\u5206\u7c7b\u7ed3\u679c\u53ef\u4ee5\u968f\u7740\u6570\u636e\u5e93\u7ec4\u6210\u53d1\u751f\u53d8\u5316\u3002<\/p>\n

\u600e\u6837\u6784\u9020\u4e00\u4efd\u80fd\u624b\u5de5\u68c0\u67e5\u7684\u8f93\u5165<\/h2>\n

\u672c\u6b21\u751f\u6210\u4e24\u4e2a\u4e5d\u5341\u6c28\u57fa\u9178\u957f\u7684\u86cb\u767d\u3002\u524d\u56db\u5341\u4e94\u4e2a\u4f4d\u7f6e\u5404\u81ea\u72ec\u7acb\u751f\u6210\uff0c\u540e\u56db\u5341\u4e94\u4e2a\u4f4d\u7f6e\u5b8c\u5168\u76f8\u540c\u3002\u5b83\u4eec\u5206\u522b\u5f52\u5c5e\u4e8e\u6559\u5b66\u79cd\u7532\u3001\u6559\u5b66\u79cd\u4e59\uff0c\u4e24\u4e2a\u79cd\u5171\u4eab\u540c\u4e00\u4e2a\u6559\u5b66\u5c5e\u3002<\/p>\n

\u5206\u7c7b\u6811\u4e2d\u7684\u7f16\u53f7\u53ea\u7528\u4e8e\u8fd9\u4e2a\u5c01\u95ed\u7684\u6559\u5b66\u4f8b\u5b50\uff0c\u6ca1\u6709\u5bf9\u5e94\u771f\u5b9e\u7269\u79cd\u7684\u542b\u4e49\u3002\u86cb\u767d\u6807\u9898\u91c7\u7528\u7a0b\u5e8f\u652f\u6301\u7684\u7f16\u53f7\u683c\u5f0f\uff0c\u5206\u7c7b\u6811\u4fdd\u5b58\u7236\u5b50\u5173\u7cfb\uff0c\u540d\u79f0\u6587\u4ef6\u4fdd\u5b58\u663e\u793a\u540d\u79f0\uff0c\u4e09\u8005\u4fdd\u6301\u4e00\u81f4\u3002<\/p>\n

\u6211\u4eec\u4ece\u4e24\u4e2a\u86cb\u767d\u4e2d\u53d6\u51fa\u4e0d\u540c\u7247\u6bb5\uff0c\u518d\u6309\u6307\u5b9a\u5bc6\u7801\u5b50\u7f16\u7801\u6210\u516b\u6761\u6838\u9178\u8bfb\u6bb5\u3002\u7532\u7684\u72ec\u6709\u7247\u6bb5\u751f\u6210\u4e09\u79cd\u7248\u672c\uff0c\u5206\u522b\u91c7\u7528\u521d\u59cb\u5bc6\u7801\u5b50\u3001\u540c\u4e49\u5bc6\u7801\u5b50\u548c\u521d\u59cb\u5e8f\u5217\u7684\u53cd\u5411\u4e92\u8865\u3002\u53e6\u6709\u4e59\u7684\u72ec\u6709\u7247\u6bb5\u3001\u4e24\u8005\u5171\u4eab\u7247\u6bb5\u3001\u5e26\u4e00\u4e2a\u6c28\u57fa\u9178\u6539\u53d8\u7684\u7532\u7247\u6bb5\u3001\u4e00\u4e2a\u5f88\u77ed\u7684\u7532\u7247\u6bb5\u53ca\u4e00\u6761\u65e0\u5173\u5e8f\u5217\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n\n\n\n\n
\u8bfb\u6bb5<\/th>\n\u8bbe\u8ba1\u5185\u5bb9<\/th>\n\u6838\u9178\u957f\u5ea6<\/th>\n\u4e3b\u8981\u6838\u5bf9\u76ee\u6807<\/th>\n<\/tr>\n<\/thead>\n
a_exact<\/td>\n\u7532\u7684\u524d 40 \u4e2a\u6c28\u57fa\u9178<\/td>\n120 nt<\/td>\n\u5b8c\u5168\u5339\u914d<\/td>\n<\/tr>\n
a_synonymous<\/td>\n\u540c\u4e00\u86cb\u767d\u7247\u6bb5\u6362\u7528\u540c\u4e49\u5bc6\u7801\u5b50<\/td>\n120 nt<\/td>\n\u7ffb\u8bd1\u540e\u7684\u5bf9\u5e94\u5173\u7cfb<\/td>\n<\/tr>\n
a_reverse_complement<\/td>\n\u521d\u59cb\u7532\u8bfb\u6bb5\u7684\u53cd\u5411\u4e92\u8865<\/td>\n120 nt<\/td>\n\u65b9\u5411\u5904\u7406<\/td>\n<\/tr>\n
b_exact<\/td>\n\u4e59\u7684\u524d 40 \u4e2a\u6c28\u57fa\u9178<\/td>\n120 nt<\/td>\n\u72ec\u7acb\u5019\u9009<\/td>\n<\/tr>\n
shared<\/td>\n\u4e24\u4e2a\u86cb\u767d\u76f8\u540c\u7684\u540e\u534a\u6bb5<\/td>\n135 nt<\/td>\n\u5171\u4eab\u5339\u914d\u4e0e\u5206\u7c7b\u5c42\u7ea7<\/td>\n<\/tr>\n
one_aa_change<\/td>\n\u7532\u7247\u6bb5\u4e2d\u6539\u53d8\u4e00\u4e2a\u6c28\u57fa\u9178<\/td>\n120 nt<\/td>\n\u6a21\u5f0f\u4e0e\u95e8\u69db<\/td>\n<\/tr>\n
short_10aa<\/td>\n\u7532\u7684\u524d 10 \u4e2a\u6c28\u57fa\u9178<\/td>\n30 nt<\/td>\n\u957f\u5ea6\u4e0d\u8db3<\/td>\n<\/tr>\n
unrelated<\/td>\n\u72ec\u7acb\u751f\u6210\u7684\u86cb\u767d\u7247\u6bb5<\/td>\n120 nt<\/td>\n\u65e0\u5173\u8f93\u5165<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u8bfb\u6bb5\u6570\u91cf\u5f88\u5c0f\uff0c\u56e0\u6b64\u53ef\u4ee5\u4e3a\u6bcf\u4e00\u884c\u8f93\u51fa\u627e\u5230\u660e\u786e\u7684\u8f93\u5165\u6765\u6e90\u3002\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u540e\uff0c\u4e0b\u8f7d\u5305\u4f1a\u91cd\u65b0\u751f\u6210\u76f8\u540c\u5e8f\u5217\uff0c\u4fbf\u4e8e\u8bfb\u8005\u81ea\u884c\u6539\u53d8\u53c2\u8003\u3001\u5339\u914d\u95e8\u69db\u6216\u5bc6\u7801\u5b50\u65b9\u6848\u3002<\/p>\n

\u5b89\u88c5\u3001\u5efa\u5e93\u4e0e\u7b2c\u4e00\u6b21\u5b9e\u9645\u5206\u7c7b<\/h2>\n

\u672c\u6b21\u56fa\u5b9a\u4f5c\u8005\u4ed3\u5e93\u63d0\u4ea4 570830d737ce41d233c6a1269a9bd46b6353bea1<\/code>\uff0c\u6309\u9879\u76ee\u8bf4\u660e\u7f16\u8bd1\u6e90\u4ee3\u7801\u3002\u751f\u6210\u7684\u7a0b\u5e8f\u62a5\u544a\u7248\u672c\u4e3a 1.10.4\uff0c\u4e8c\u8fdb\u5236\u6821\u9a8c\u503c\u4fdd\u5b58\u5728\u7ed3\u679c\u6587\u4ef6\u4e2d\u3002\u5b98\u65b9\u6e90\u7801\u5b89\u88c5\u65b9\u6cd5<\/a><\/p>\n

\u86cb\u767d\u5e93\u7d22\u5f15\u5206\u4e24\u6b65\u6784\u5efa\uff0c\u5148\u751f\u6210\u53d8\u6362\u4e0e\u76f8\u5173\u6587\u4ef6\uff0c\u518d\u751f\u6210\u5206\u7c7b\u4f7f\u7528\u7684\u7d22\u5f15\u3002\u4e0b\u9762\u5c55\u793a\u672c\u6b21\u8c03\u7528\u5f62\u5f0f\uff0c\u5176\u4e2d\u5b57\u6bcd\u8868\u53ea\u5305\u542b\u4e8c\u5341\u79cd\u6807\u51c6\u6c28\u57fa\u9178\u3002<\/p>\n

kaiju-mkbwt -n 2 -l 1 \\\r\n  -a ACDEFGHIKLMNPQRSTVWY \\\r\n  -o both both.faa\r\nkaiju-mkfmi both\r\n<\/code><\/pre>\n
\u672c\u6b21\u7b2c\u4e00\u6b21\u5bf9\u6781\u5c0f\u7684\u6559\u5b66\u6587\u4ef6\u5efa\u7d22\u5f15\u65f6\uff0c\u7a0b\u5e8f\u63d0\u793a\u5e8f\u5217\u5206\u914d\u7a7a\u95f4\u4e0d\u8db3\u3002\u7ed9\u5efa\u7d22\u5f15\u547d\u4ee4\u663e\u5f0f\u6307\u5b9a\u957f\u5ea6\u9884\u7b97\u540e\uff0c\u4e24\u4e2a\u5c0f\u7d22\u5f15\u5747\u6210\u529f\u751f\u6210\u3002\u8fd9\u91cc\u7684\u957f\u5ea6\u9884\u7b97\u4ee5\u767e\u4e07\u5b57\u7b26\u4e3a\u5355\u4f4d\uff0c\u4e0d\u80fd\u628a\u53c2\u6570\u4e2d\u7684\u4e00\u76f4\u63a5\u7406\u89e3\u4e3a\u4e00\u4e2a\u6c28\u57fa\u9178\u3002<\/p>\n
\u8fd9\u9879\u7ecf\u5386\u63d0\u9192\u6211\u4eec\uff0c\u62a5\u9519\u9700\u8981\u7ed3\u5408\u8f93\u5165\u89c4\u6a21\u548c\u5177\u4f53\u6b65\u9aa4\u7406\u89e3\u3002\u5148\u770b\u54ea\u6761\u547d\u4ee4\u5931\u8d25\u3001\u8f93\u5165\u6587\u4ef6\u662f\u5426\u5b8c\u6574\u3001\u53c2\u6570\u542b\u4e49\u662f\u4ec0\u4e48\uff0c\u518d\u5224\u65ad\u539f\u56e0\u3002\u672c\u6b21\u5904\u7406\u65b9\u5f0f\u53ea\u9488\u5bf9\u8fd9\u4e2a\u5c0f\u6587\u4ef6\u5b9e\u9a8c\uff0c\u6b63\u5f0f\u53c2\u8003\u5e93\u5e94\u6309\u5b9e\u9645\u5e8f\u5217\u91cf\u8bbe\u7f6e\u76f8\u5e94\u9884\u7b97\u3002<\/p>\n
\u7b2c\u4e00\u7ec4\u4f7f\u7528\u4e24\u4e2a\u86cb\u767d\u53c2\u8003\uff0c\u9009\u62e9\u5b8c\u5168\u5339\u914d\u6a21\u5f0f\uff0c\u5c06\u6700\u5c0f\u5339\u914d\u957f\u5ea6\u8bbe\u4e3a\u4e8c\u5341\u4e94\u3002\u8be6\u7ec6\u8f93\u51fa\u4f1a\u4fdd\u7559\u5339\u914d\u4fe1\u606f\uff0c\u4fbf\u4e8e\u9010\u6761\u68c0\u67e5\u3002<\/p>\n
kaiju -t nodes.dmp -f both.fmi \\\r\n  -i reads.fna -o mem25.out \\\r\n  -a mem -m 25 -z 2 -v\r\n<\/code><\/pre>\n
\u6838\u9178\u4e00\u81f4\u6027\u53ea\u6709\u7ea6\u767e\u5206\u4e4b\u516d\u5341\u516b\uff0c\u4e3a\u4ec0\u4e48\u4ecd\u7136\u5339\u914d<\/h2>\n
\u7532\u7684\u521d\u59cb\u8bfb\u6bb5\u4e0e\u540c\u4e49\u5bc6\u7801\u5b50\u7248\u672c\u957f\u5ea6\u5747\u4e3a\u4e00\u767e\u4e8c\u5341\u4e2a\u78b1\u57fa\uff0c\u9010\u4f4d\u6bd4\u8f83\u53ea\u6709\u516b\u5341\u4e8c\u4e2a\u78b1\u57fa\u76f8\u540c\uff0c\u4e00\u81f4\u6bd4\u4f8b\u7ea6\u4e3a\u767e\u5206\u4e4b\u516d\u5341\u516b\u70b9\u4e09\u4e09\u3002\u6309\u8bbe\u8ba1\u7ffb\u8bd1\u540e\uff0c\u4e24\u6761\u8bfb\u6bb5\u5bf9\u5e94\u540c\u4e00\u4e2a\u56db\u5341\u6c28\u57fa\u9178\u7247\u6bb5\u3002<\/p>\n
\u5b9e\u9645\u8f93\u51fa\u4e2d\uff0c\u521d\u59cb\u7248\u672c\u3001\u540c\u4e49\u7248\u672c\u548c\u53cd\u5411\u4e92\u8865\u7248\u672c\u90fd\u88ab\u5206\u5230\u6559\u5b66\u79cd\u7532\u3002\u5b8c\u5168\u5339\u914d\u6a21\u5f0f\u7684\u8be6\u7ec6\u5217\u5747\u62a5\u544a\u56db\u5341\u6c28\u57fa\u9178\u7684\u652f\u6301\uff0c\u4e0e\u8f93\u5165\u8bbe\u8ba1\u4e00\u81f4\u3002<\/p>\n
\u8fd9\u4e2a\u7ed3\u679c\u53ef\u4ee5\u7528\u6765\u7406\u89e3\u86cb\u767d\u641c\u7d22\u7684\u4e00\u4e2a\u5177\u4f53\u4f18\u52bf\u3002\u6838\u9178\u5b57\u9762\u5dee\u5f02\u53ef\u80fd\u6765\u81ea\u5bc6\u7801\u5b50\u53d8\u5316\uff0c\u7ffb\u8bd1\u4e4b\u540e\u4ecd\u7136\u80fd\u591f\u4fdd\u7559\u76f8\u540c\u7684\u86cb\u767d\u7247\u6bb5\u3002\u8fd9\u91cc\u7684\u4e00\u81f4\u6bd4\u4f8b\u662f\u4e24\u6761\u4eba\u4e3a\u7f16\u7801\u8bfb\u6bb5\u4e4b\u95f4\u7684\u6bd4\u8f83\u503c\uff0c\u4e0d\u4ee3\u8868 Kaiju \u5728\u771f\u5b9e\u6570\u636e\u4e0a\u5b58\u5728\u67d0\u4e2a\u901a\u7528\u7684\u6838\u9178\u8bc6\u522b\u4e0b\u9650\u3002<\/p>\n
\u8fc1\u79fb\u5230\u5b9e\u9645\u8bfb\u6bb5\u65f6\uff0c\u8fd8\u8981\u8003\u8651\u8bfb\u957f\u3001\u7f16\u7801\u533a\u57df\u6bd4\u4f8b\u3001\u6d4b\u5e8f\u9519\u8bef\u548c\u53c2\u8003\u8986\u76d6\u3002\u5c24\u5176\u662f\u63d2\u5165\u7f3a\u5931\u53ef\u80fd\u6539\u53d8\u7ffb\u8bd1\u540e\u7684\u7247\u6bb5\u7ed3\u6784\uff0c\u672c\u6b21\u4ec5\u6d4b\u8bd5\u4e86\u540c\u4e49\u7f16\u7801\u4e0e\u4e00\u4e2a\u6c28\u57fa\u9178\u66ff\u6362\uff0c\u5176\u4ed6\u9519\u8bef\u5f62\u5f0f\u9700\u8981\u53e6\u5916\u8bbe\u8ba1\u5bf9\u7167\u3002<\/p>\n
\u5171\u4eab\u7247\u6bb5\u4e3a\u4ec0\u4e48\u88ab\u5f52\u5230\u5c5e<\/h2>\n
\u5171\u4eab\u8bfb\u6bb5\u5bf9\u5e94\u56db\u5341\u4e94\u4e2a\u6c28\u57fa\u9178\uff0c\u5728\u4e24\u4e2a\u86cb\u767d\u4e2d\u90fd\u5b58\u5728\u5b8c\u5168\u76f8\u540c\u7684\u5339\u914d\u3002\u8be6\u7ec6\u8f93\u51fa\u5217\u51fa\u4e86\u4e24\u4e2a\u5019\u9009\u79cd\u7684\u7f16\u53f7\uff0c\u6700\u7ec8\u5206\u7c7b\u7f16\u53f7\u5219\u6307\u5411\u5b83\u4eec\u5171\u540c\u7684\u5c5e\u3002<\/p>\n
\u8fd9\u6761\u8bfb\u6bb5\u5df2\u7ecf\u83b7\u5f97\u5206\u7c7b\u652f\u6301\uff0c\u53ea\u662f\u652f\u6301\u8303\u56f4\u65e0\u6cd5\u7ec6\u5316\u5230\u4e24\u4e2a\u5019\u9009\u79cd\u4e2d\u7684\u67d0\u4e00\u4e2a\u3002\u7b2c\u4e00\u7ec4\u516b\u6761\u8bfb\u6bb5\u4e2d\uff0c\u4e09\u6761\u5f52\u5230\u7532\u79cd\uff0c\u4e00\u6761\u5f52\u5230\u4e59\u79cd\uff0c\u4e00\u6761\u5f52\u5230\u5171\u540c\u5c5e\uff0c\u8fd8\u6709\u4e09\u6761\u672a\u5206\u7c7b\u3002<\/p>\n
\u6211\u4eec\u8fdb\u4e00\u6b65\u8fd0\u884c\u7a0b\u5e8f\u81ea\u5e26\u7684\u6c47\u603b\u5de5\u5177\uff0c\u5206\u522b\u751f\u6210\u79cd\u7ea7\u548c\u5c5e\u7ea7\u8868\u683c\u3002\u8fd9\u6837\u53ef\u4ee5\u770b\u5230\u540c\u4e00\u6279\u9010\u8bfb\u6bb5\u7ed3\u679c\u5728\u4e24\u4e2a\u5c42\u7ea7\u4e2d\u7684\u4e0d\u540c\u8868\u8fbe\u3002<\/p>\n
kaiju2table -t nodes.dmp -n names.dmp \\\r\n  -r species -o mem25-species.tsv mem25.out\r\nkaiju2table -t nodes.dmp -n names.dmp \\\r\n  -r genus -o mem25-genus.tsv mem25.out\r\n<\/code><\/pre>\n
\u79cd\u7ea7\u8868\u4e2d\uff0c\u7532\u79cd\u5360\u5168\u90e8\u516b\u6761\u8bfb\u6bb5\u7684\u767e\u5206\u4e4b\u4e09\u5341\u4e03\u70b9\u4e94\uff0c\u4e59\u79cd\u5360\u767e\u5206\u4e4b\u5341\u4e8c\u70b9\u4e94\u3002\u53e6\u6709\u767e\u5206\u4e4b\u5341\u4e8c\u70b9\u4e94\u5df2\u7ecf\u5206\u7c7b\uff0c\u4f46\u65e0\u6cd5\u5206\u914d\u5230\u79cd\u7ea7\uff0c\u8fd8\u6709\u767e\u5206\u4e4b\u4e09\u5341\u4e03\u70b9\u4e94\u672a\u5206\u7c7b\u3002<\/p>\n
\u5c5e\u7ea7\u8868\u4e2d\uff0c\u524d\u9762\u4e94\u6761\u5df2\u6709\u5206\u7c7b\u652f\u6301\u7684\u8bfb\u6bb5\u90fd\u6c47\u603b\u5230\u540c\u4e00\u5c5e\uff0c\u5360\u767e\u5206\u4e4b\u516d\u5341\u4e8c\u70b9\u4e94\u3002\u8fd9\u7ec4\u7ed3\u679c\u8bf4\u660e\uff0c\u6c47\u603b\u8868\u91cc\u201c\u65e0\u6cd5\u5230\u8fbe\u6240\u9009\u5c42\u7ea7\u201d\u548c\u201c\u5b8c\u5168\u672a\u5206\u7c7b\u201d\u5bf9\u5e94\u4e0d\u540c\u60c5\u51b5\uff0c\u4fdd\u7559\u8fd9\u4e24\u7c7b\u8bb0\u5f55\u6709\u52a9\u4e8e\u7406\u89e3\u7ed3\u679c\u7ec4\u6210\u3002<\/p>\n
\u53c2\u8003\u5e93\u7f3a\u5c11\u5019\u9009\u65f6\uff0c\u5206\u7c7b\u5c42\u7ea7\u4f1a\u53d1\u751f\u4ec0\u4e48\u53d8\u5316<\/h2>\n
\u7b2c\u4e09\u7c7b\u5bf9\u7167\u4fdd\u6301\u8bfb\u6bb5\u3001\u5206\u7c7b\u6811\u548c\u5339\u914d\u53c2\u6570\u4e0d\u53d8\uff0c\u4ec5\u4ece\u86cb\u767d\u53c2\u8003\u4e2d\u79fb\u9664\u4e59\u7684\u86cb\u767d\u3002\u968f\u540e\u91cd\u65b0\u5efa\u7d22\u5f15\uff0c\u91cd\u65b0\u8fd0\u884c\u540c\u6837\u7684\u5b8c\u5168\u5339\u914d\u5206\u7c7b\u3002<\/p>\n
\u5b9e\u9645\u7ed3\u679c\u4e2d\uff0c\u5171\u4eab\u7247\u6bb5\u4ece\u5171\u540c\u5c5e\u53d8\u4e3a\u6559\u5b66\u79cd\u7532\u3002\u8be6\u7ec6\u8f93\u51fa\u53ea\u5269\u7532\u79cd\u8fd9\u4e2a\u5019\u9009\uff0c\u5339\u914d\u957f\u5ea6\u4ecd\u4e3a\u56db\u5341\u4e94\u4e2a\u6c28\u57fa\u9178\u3002\u4e59\u7684\u72ec\u6709\u8bfb\u6bb5\u5219\u4ece\u539f\u5148\u7684\u4e59\u79cd\u53d8\u4e3a\u672a\u5206\u7c7b\u3002<\/p>\n
\u5171\u4eab\u7247\u6bb5\u7684\u5e8f\u5217\u6ca1\u6709\u6539\u53d8\uff0c\u5339\u914d\u957f\u5ea6\u4e5f\u6ca1\u6709\u589e\u52a0\uff0c\u5206\u7c7b\u5c42\u7ea7\u5374\u53d8\u7ec6\u4e86\u3002\u901a\u8fc7\u8fd9\u4e2a\u53ef\u63a7\u5bf9\u7167\uff0c\u53ef\u4ee5\u660e\u786e\u770b\u5230\u53d8\u5316\u6765\u81ea\u53c2\u8003\u5019\u9009\u96c6\u5408\u3002\u6570\u636e\u5e93\u4e2d\u53ea\u4fdd\u7559\u4e00\u4e2a\u5019\u9009\uff0c\u4f1a\u6539\u53d8\u7a0b\u5e8f\u80fd\u591f\u6bd4\u8f83\u7684\u8303\u56f4\u3002<\/p>\n
\u56e0\u6b64\uff0c\u6bd4\u8f83\u4e24\u4e2a\u9879\u76ee\u7684\u5206\u7c7b\u7ed3\u679c\u65f6\uff0c\u9700\u8981\u4fdd\u5b58\u53c2\u8003\u86cb\u767d\u5e93\u3001\u7d22\u5f15\u548c\u5206\u7c7b\u6811\u7684\u7248\u672c\u3002\u82e5\u6570\u636e\u5e93\u66f4\u65b0\u540e\u67d0\u4e9b\u6761\u76ee\u4ece\u79cd\u53d8\u6210\u5c5e\uff0c\u4e5f\u53ef\u80fd\u662f\u52a0\u5165\u4e86\u65b0\u7684\u8fd1\u7f18\u5019\u9009\uff0c\u4f7f\u539f\u6709\u7247\u6bb5\u7684\u5171\u4eab\u5173\u7cfb\u663e\u73b0\u51fa\u6765\u3002\u89e3\u91ca\u5177\u4f53\u53d8\u5316\u65f6\uff0c\u53ef\u4ee5\u6cbf\u7528\u672c\u4f8b\u7684\u529e\u6cd5\uff0c\u56de\u5230\u8be6\u7ec6\u8f93\u51fa\u68c0\u67e5\u65b0\u589e\u6216\u6d88\u5931\u7684\u5019\u9009\u3002<\/p>\n
\u672c\u6b21\u79cd\u7ea7\u6c47\u603b\u4e2d\uff0c\u7532\u7531\u4e09\u6761\u652f\u6301\u53d8\u6210\u56db\u6761\uff0c\u5360\u6bd4\u4ece\u767e\u5206\u4e4b\u4e09\u5341\u4e03\u70b9\u4e94\u53d8\u6210\u767e\u5206\u4e4b\u4e94\u5341\u3002\u6837\u672c\u8bfb\u6bb5\u5b8c\u5168\u76f8\u540c\uff0c\u53d8\u5316\u7531\u53c2\u8003\u5e93\u5f15\u8d77\u3002\u628a\u8fd9\u7c7b\u5dee\u5f02\u76f4\u63a5\u89e3\u91ca\u4e3a\u7fa4\u843d\u4e30\u5ea6\u53d8\u5316\uff0c\u4f1a\u6f0f\u6389\u51b3\u5b9a\u7ed3\u679c\u7684\u5206\u6790\u6761\u4ef6\u3002<\/p>\n
\u957f\u5ea6\u95e8\u69db\u548c\u641c\u7d22\u6a21\u5f0f\u600e\u6837\u5206\u522b\u5f71\u54cd\u7ed3\u679c<\/h2>\n
\u5c06\u5b8c\u5168\u5339\u914d\u6a21\u5f0f\u7684\u95e8\u69db\u4ece\u4e8c\u5341\u4e94\u63d0\u9ad8\u5230\u56db\u5341\u4e00\u4ee5\u540e\uff0c\u539f\u5148\u56db\u5341\u6c28\u57fa\u9178\u957f\u7684\u72ec\u6709\u5339\u914d\u5168\u90e8\u4f4e\u4e8e\u95e8\u69db\u3002\u56db\u5341\u4e94\u6c28\u57fa\u9178\u957f\u7684\u5171\u4eab\u7247\u6bb5\u4ecd\u7136\u4fdd\u7559\uff0c\u6700\u7ec8\u4ecd\u7136\u53ea\u80fd\u5f52\u5230\u5c5e\u3002\u95e8\u69db\u53d8\u9ad8\u5e76\u6ca1\u6709\u81ea\u52a8\u63d0\u9ad8\u672c\u4f8b\u7684\u79cd\u7ea7\u5206\u8fa8\u7387\u3002<\/p>\n
\u5e26\u4e00\u4e2a\u6c28\u57fa\u9178\u6539\u53d8\u7684\u7532\u8bfb\u6bb5\u63d0\u4f9b\u4e86\u53e6\u4e00\u7ec4\u68c0\u67e5\u673a\u4f1a\u3002\u6539\u53d8\u4f4d\u4e8e\u56db\u5341\u6c28\u57fa\u9178\u7247\u6bb5\u7684\u4e2d\u95f4\uff0c\u5c06\u6700\u957f\u8fde\u7eed\u5b8c\u5168\u76f8\u540c\u7247\u6bb5\u5206\u6210\u8f83\u77ed\u7684\u90e8\u5206\u3002\u6211\u4eec\u5728\u4e24\u79cd\u6a21\u5f0f\u4e0b\u5206\u522b\u6d4b\u8bd5\u957f\u5ea6\u95e8\u69db\u5341\u4e00\u548c\u4e8c\u5341\u4e94\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
\u6761\u4ef6<\/th>\n\u542b\u4e00\u4e2a\u6c28\u57fa\u9178\u6539\u53d8\u7684\u8bfb\u6bb5<\/th>\n\u8be6\u7ec6\u8f93\u51fa\u7b2c\u56db\u5217<\/th>\n<\/tr>\n<\/thead>\n
MEM\uff0c\u95e8\u69db 25<\/td>\n\u672a\u5206\u7c7b<\/td>\n\u65e0\u5339\u914d\u8bb0\u5f55<\/td>\n<\/tr>\n
MEM\uff0c\u95e8\u69db 11<\/td>\n\u5206\u5230\u7532\u79cd<\/td>\n\u5b8c\u5168\u5339\u914d\u957f\u5ea6 20<\/td>\n<\/tr>\n
Greedy\uff0c\u95e8\u69db 25<\/td>\n\u5206\u5230\u7532\u79cd<\/td>\n\u5339\u914d\u5206\u6570 226<\/td>\n<\/tr>\n
Greedy\uff0c\u95e8\u69db 11<\/td>\n\u5206\u5230\u7532\u79cd<\/td>\n\u5339\u914d\u5206\u6570 226<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8fd9\u5f20\u8868\u6765\u81ea\u989d\u5916\u5b9e\u8dd1\uff0c\u907f\u514d\u628a\u540c\u65f6\u6539\u53d8\u6a21\u5f0f\u548c\u95e8\u69db\u7684\u7ed3\u679c\u6df7\u5728\u4e00\u8d77\u89e3\u91ca\u3002\u5b8c\u5168\u5339\u914d\u6a21\u5f0f\u5bf9\u8fde\u7eed\u7247\u6bb5\u957f\u5ea6\u4f5c\u51fa\u54cd\u5e94\uff0c\u5141\u8bb8\u6269\u5c55\u7684\u6a21\u5f0f\u5728\u672c\u4f8b\u4e2d\u4fdd\u7559\u4e86\u542b\u66ff\u6362\u7684\u652f\u6301\u3002<\/p>\n
\u8fd8\u9700\u8981\u6ce8\u610f\u8be6\u7ec6\u8f93\u51fa\u7b2c\u56db\u5217\u7684\u5355\u4f4d\u3002\u5b8c\u5168\u5339\u914d\u6a21\u5f0f\u4e2d\u770b\u5230\u7684\u4e8c\u5341\u4ee3\u8868\u5339\u914d\u957f\u5ea6\uff0c\u53e6\u4e00\u6a21\u5f0f\u4e2d\u7684\u4e8c\u767e\u4e8c\u5341\u516d\u4ee3\u8868\u5206\u6570\u3002\u4e24\u8005\u4e0d\u80fd\u76f4\u63a5\u653e\u5728\u540c\u4e00\u6761\u957f\u5ea6\u5750\u6807\u4e0a\u6bd4\u8f83\u3002\u8bfb\u53d6\u7ed3\u679c\u65f6\uff0c\u5148\u8bb0\u5f55\u8fd0\u884c\u6a21\u5f0f\uff0c\u518d\u7ed9\u5b57\u6bb5\u5199\u6e05\u542b\u4e49\u3002<\/p>\n
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\u6c47\u603b\u524d\uff0c\u5148\u6838\u5bf9\u8bfb\u6bb5\u540d\u79f0\u4e0e\u8f93\u51fa\u5355\u4f4d<\/h2>\n
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