{"id":72755,"date":"2026-11-15T10:34:50","date_gmt":"2026-11-15T02:34:50","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72755"},"modified":"2026-11-15T10:34:50","modified_gmt":"2026-11-15T02:34:50","slug":"instrain-popani-consensus-allele-frequency-base-quality","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/instrain-popani-consensus-allele-frequency-base-quality\/","title":{"rendered":"inStrain \u7684 popANI \u4e3a 1\uff0c\u4e24\u4e2a\u6837\u672c\u5c31\u5b8c\u5168\u76f8\u540c\u5417\uff1f\u5b9e\u8dd1\u7b49\u4f4d\u57fa\u56e0\u6bd4\u4f8b\u3001\u5171\u8bc6\u5dee\u5f02\u4e0e\u78b1\u57fa\u8d28\u91cf"},"content":{"rendered":"
\n

\u4e24\u4e2a\u6837\u672c\u7684\u4f18\u52bf\u78b1\u57fa\u4e0d\u540c\uff0c\u4e3a\u4ec0\u4e48 inStrain \u4ecd\u7136\u62a5\u544a\u7fa4\u4f53\u5c42\u9762\u7684\u4e00\u81f4\u6027\u4e3a\u4e00\uff1f\u7406\u89e3\u8fd9\u4e2a\u95ee\u9898\uff0c\u9700\u8981\u540c\u65f6\u67e5\u770b\u6bcf\u4e2a\u4f4d\u70b9\u4fdd\u7559\u4e86\u54ea\u4e9b\u7b49\u4f4d\u57fa\u56e0\u3001\u5404\u81ea\u7684\u652f\u6301\u6570\u91cf\uff0c\u4ee5\u53ca\u4e24\u4e2a\u6837\u672c\u7a76\u7adf\u6bd4\u8f83\u4e86\u591a\u5c11\u4f4d\u7f6e\u3002<\/p>\n

\u672c\u6587\u5b89\u88c5 inStrain 1.12.0\uff0c\u751f\u6210\u56db\u7ec4\u914d\u5bf9\u6bd4\u5bf9\u6570\u636e\uff0c\u5b9e\u9645\u5b8c\u6210\u56db\u6b21 profile \u548c\u4e24\u6b21 compare\u3002\u5b9e\u9a8c\u628a\u53c2\u8003\u957f\u5ea6\u3001\u8bfb\u6bb5\u6570\u91cf\u4e0e\u4f4d\u7f6e\u56fa\u5b9a\u4e0b\u6765\uff0c\u53ea\u6539\u53d8\u4e00\u4e2a\u4f4d\u70b9\u7684\u78b1\u57fa\u7ec4\u6210\u6216\u78b1\u57fa\u8d28\u91cf\u3002\u6700\u7ec8\u8f93\u51fa\u53ef\u4ee5\u9010\u4f4d\u590d\u7b97\uff0c\u5e2e\u52a9\u8bfb\u8005\u7406\u89e3\u4e0d\u540c\u6307\u6807\u63cf\u8ff0\u7684\u542b\u4e49\u3002<\/p>\n

\"popANI
\u56db\u7ec4 profile \u4e0e\u4e24\u7ec4 compare \u5b9e\u9645\u6838\u5bf9<\/figcaption><\/figure>\n

\u4e24\u7c7b\u4e00\u81f4\u6027\u6307\u6807\u5728\u56de\u7b54\u4ec0\u4e48\u95ee\u9898<\/h2>\n

\u5b8f\u57fa\u56e0\u7ec4\u8bfb\u6bb5\u5f80\u5f80\u6765\u81ea\u540c\u4e00\u7c7b\u751f\u7269\u7684\u591a\u4e2a\u7ec6\u80de\uff0c\u56e0\u6b64\u5728\u4e00\u4e2a\u53c2\u8003\u4f4d\u7f6e\u4e0a\u53ef\u80fd\u540c\u65f6\u89c2\u5bdf\u5230\u4e0d\u540c\u78b1\u57fa\u3002\u4ee5\u6570\u91cf\u6700\u591a\u7684\u78b1\u57fa\u6784\u5efa\u5171\u8bc6\uff0c\u53ef\u4ee5\u5f97\u5230\u4e00\u4e2a\u7b80\u5316\u8868\u793a\uff0c\u4f46\u8fd9\u4e2a\u8868\u793a\u4f1a\u7565\u53bb\u4ecd\u7136\u5b58\u5728\u7684\u6b21\u8981\u7b49\u4f4d\u57fa\u56e0\u3002<\/p>\n

inStrain \u540c\u65f6\u63d0\u4f9b\u5171\u8bc6\u5c42\u9762\u4e0e\u7fa4\u4f53\u5c42\u9762\u7684\u6bd4\u8f83\u3002\u5171\u8bc6\u5c42\u9762\u5173\u6ce8\u4e24\u4e2a\u6837\u672c\u7684\u4f18\u52bf\u78b1\u57fa\u662f\u5426\u76f8\u540c\uff0c\u7fa4\u4f53\u5c42\u9762\u8fdb\u4e00\u6b65\u8003\u8651\u80fd\u591f\u901a\u8fc7\u6761\u4ef6\u7684\u7b49\u4f4d\u57fa\u56e0\u662f\u5426\u5171\u4eab\u3002\u9605\u8bfb\u7ed3\u679c\u65f6\uff0c\u5c06\u4e24\u79cd\u6307\u6807\u4e0e\u5b9e\u9645\u78b1\u57fa\u8ba1\u6570\u653e\u5728\u4e00\u8d77\uff0c\u901a\u5e38\u66f4\u5bb9\u6613\u89e3\u91ca\u5dee\u5f02\u3002\u5b98\u65b9\u6838\u5fc3\u6982\u5ff5\u8bf4\u660e<\/a><\/p>\n

\u5de5\u5177\u8f93\u5165\u901a\u5e38\u5305\u542b\u53c2\u8003\u5e8f\u5217\u4ee5\u53ca\u8bfb\u6bb5\u6bd4\u5bf9\u5230\u8be5\u53c2\u8003\u540e\u5f97\u5230\u7684 BAM\u3002\u53c2\u8003\u540d\u79f0\u3001\u53c2\u8003\u5185\u5bb9\u4e0e\u6bd4\u5bf9\u5934\u5fc5\u987b\u5bf9\u5e94\u3002\u591a\u4e2a\u6837\u672c\u8981\u6bd4\u8f83\u540c\u4e00\u6279\u4f4d\u7f6e\uff0c\u4e5f\u9700\u8981\u4fdd\u6301\u53c2\u8003\u4f53\u7cfb\u4e00\u81f4\u3002\u5b98\u65b9\u8f93\u5165\u4e0e\u8fd0\u884c\u8bf4\u660e<\/a><\/p>\n

\u56db\u7ec4\u8f93\u5165\u53ea\u5728\u4e00\u4e2a\u4f4d\u7f6e\u4e0a\u6709\u6240\u6539\u53d8<\/h2>\n

\u672c\u6b21\u6559\u5b66\u53c2\u8003\u957f\u4e24\u5343\u78b1\u57fa\uff0c\u4f7f\u7528\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u751f\u6210\u3002\u6bcf\u7ec4\u6570\u636e\u5305\u542b\u4e94\u767e\u5bf9\u8bfb\u6bb5\uff0c\u4e00\u5171\u4e00\u5343\u6761\u8bb0\u5f55\uff0c\u6bcf\u6761\u957f\u4e00\u767e\u78b1\u57fa\u3002\u914d\u5bf9\u6210\u5458\u76f8\u90bb\u6392\u5217\uff0c\u5728\u6574\u4e2a\u53c2\u8003\u4e0a\u5f62\u6210\u4e94\u5341\u500d\u5747\u5300\u8986\u76d6\u3002<\/p>\n

\u53c2\u8003\u7684\u7b2c\u4e94\u5341\u4e94\u4e2a\u4f4d\u7f6e\u662f\u78b1\u57fa T\u3002\u6211\u4eec\u5728\u7532\u6837\u672c\u4e2d\u5b89\u6392\u56db\u5341\u6761 T \u548c\u5341\u6761 A\uff0c\u5728\u4e59\u6837\u672c\u4e2d\u5b89\u6392\u5341\u6761 T \u548c\u56db\u5341\u6761 A\uff0c\u5728\u4e19\u6837\u672c\u4e2d\u5b89\u6392\u4e94\u5341\u6761 C\u3002\u4e09\u4e2a\u6837\u672c\u7684\u5176\u4ed6\u4f4d\u7f6e\u5168\u90e8\u4e0e\u53c2\u8003\u4e00\u81f4\u3002<\/p>\n

\u7b2c\u56db\u7ec4\u4fdd\u7559\u7532\u7684\u5e8f\u5217\u4e0e\u6bd4\u5bf9\u4f4d\u7f6e\uff0c\u53ea\u628a\u8be5\u4f4d\u70b9\u5341\u6761 A \u7684\u78b1\u57fa\u8d28\u91cf\u4ece\u56db\u5341\u964d\u5230\u4e8c\u5341\u3002\u5176\u4ed6\u78b1\u57fa\u8d28\u91cf\u4fdd\u6301\u56db\u5341\uff0c\u8bfb\u6bb5\u6bd4\u5bf9\u8d28\u91cf\u7edf\u4e00\u4e3a\u516d\u5341\u3002\u8fd9\u6837\u80fd\u591f\u72ec\u7acb\u89c2\u5bdf\u4f4d\u70b9\u8d28\u91cf\u8fc7\u6ee4\u7684\u5f71\u54cd\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n
\u8f93\u5165\u6837\u672c<\/th>\n\u76ee\u6807\u4f4d\u7f6e T<\/th>\n\u76ee\u6807\u4f4d\u7f6e A<\/th>\n\u76ee\u6807\u4f4d\u7f6e C<\/th>\nA \u7684\u78b1\u57fa\u8d28\u91cf<\/th>\n<\/tr>\n<\/thead>\n
\u7532<\/td>\n40<\/td>\n10<\/td>\n0<\/td>\nQ40<\/td>\n<\/tr>\n
\u4e59<\/td>\n10<\/td>\n40<\/td>\n0<\/td>\nQ40<\/td>\n<\/tr>\n
\u4e19<\/td>\n0<\/td>\n0<\/td>\n50<\/td>\n\u4e0d\u9002\u7528<\/td>\n<\/tr>\n
\u7532\u7684\u4f4e\u8d28\u91cf\u5bf9\u7167<\/td>\n40<\/td>\n10<\/td>\n0<\/td>\nQ20<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u8fd9\u4e9b\u662f\u76f4\u63a5\u6784\u9020\u7684\u6559\u5b66\u6bd4\u5bf9\u8bb0\u5f55\uff0c\u6ca1\u6709\u7ecf\u8fc7\u771f\u5b9e\u8bfb\u6bb5\u641c\u7d22\u3002\u5b83\u4eec\u7528\u6765\u9a8c\u8bc1\u5df2\u77e5\u8f93\u5165\u4e0b\u7684\u8fc7\u6ee4\u3001\u53d8\u5f02\u7edf\u8ba1\u548c\u6bd4\u8f83\u884c\u4e3a\u3002\u771f\u5b9e\u9879\u76ee\u8fd8\u9700\u8981\u8003\u8651\u9519\u914d\u4f4d\u7f6e\u3001\u91cd\u590d\u533a\u57df\u548c\u9519\u8bef\u6bd4\u5bf9\u7b49\u989d\u5916\u6765\u6e90\u3002<\/p>\n

\u5b89\u88c5\u4e0e\u8fd0\u884c\u65f6\u9700\u8981\u56fa\u5b9a\u54ea\u4e9b\u6761\u4ef6<\/h2>\n

\u672c\u6b21\u5728\u72ec\u7acb\u7684 Python 3.10 \u73af\u5883\u4e2d\u901a\u8fc7\u5b98\u65b9\u63d0\u4f9b\u7684\u5b89\u88c5\u65b9\u5f0f\u5b89\u88c5\u56fa\u5b9a\u7248\u672c\uff0c\u914d\u5957 pysam \u4e3a 0.24.1\u3002\u8bfb\u6bb5\u8bb0\u5f55\u3001\u6392\u5e8f\u548c\u7d22\u5f15\u7531 pysam \u5b9e\u9645\u751f\u6210\uff0c\u968f\u540e\u8fd0\u884c\u6b63\u5e38\u7684\u547d\u4ee4\u884c\u6d41\u7a0b\u3002\u9879\u76ee\u5b89\u88c5\u8bf4\u660e<\/a><\/p>\n

python -m pip install inStrain==1.12.0\r\ninStrain profile --version\r\n\r\ninStrain profile A.bam reference.fna \\\r\n  -o A.IS -p 2 -s genomes.stb \\\r\n  --skip_plot_generation \\\r\n  --skip_mm_profiling\r\n<\/code><\/pre>\n
\u672c\u6b21\u5173\u95ed\u7ed8\u56fe\uff0c\u5e76\u5173\u95ed\u6309\u8bfb\u6bb5\u9519\u914d\u6570\u91cf\u9010\u7ea7\u5c55\u5f00\u7684\u5206\u6790\uff0c\u4ee5\u96c6\u4e2d\u68c0\u67e5\u6700\u7ec8\u4fdd\u7559\u8bfb\u6bb5\u7684\u7ed3\u679c\u3002\u4f4d\u70b9\u8986\u76d6\u95e8\u69db\u3001\u53d8\u5f02\u9891\u7387\u95e8\u69db\u3001\u78b1\u57fa\u8d28\u91cf\u95e8\u69db\u548c\u5176\u4ed6\u8fc7\u6ee4\u53c2\u6570\u4fdd\u6301\u672c\u6b21\u7248\u672c\u9ed8\u8ba4\u503c\u3002<\/p>\n
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\u7248\u672c\u540c\u6837\u5c5e\u4e8e\u5206\u6790\u6761\u4ef6\u3002\u4f5c\u8005\u7684\u66f4\u65b0\u8bb0\u5f55\u8bf4\u660e\uff0c\u7b2c\u4e00\u70b9\u5341\u4e8c\u7248\u8c03\u6574\u4e86\u53d8\u5f02\u68c0\u51fa\u7684\u9608\u503c\u884c\u4e3a\uff0c\u590d\u73b0\u66f4\u65e9\u7ed3\u679c\u65f6\u9700\u8981\u5173\u6ce8\u76f8\u5e94\u517c\u5bb9\u53c2\u6570\u3002\u672c\u6b21\u91c7\u7528\u8fd9\u4e00\u7248\u672c\u7684\u6b63\u5e38\u9ed8\u8ba4\u884c\u4e3a\u3002\u5b98\u65b9\u7248\u672c\u53d8\u66f4\u8bb0\u5f55<\/a><\/p>\n
\u5148\u6838\u5bf9\u8bfb\u6bb5\u662f\u5426\u771f\u6b63\u8fdb\u5165\u4e86\u7edf\u8ba1<\/h2>\n
\u56db\u7ec4\u8f93\u5165\u7684\u914d\u5bf9\u8fc7\u6ee4\u7ed3\u679c\u90fd\u4fdd\u7559\u4e94\u767e\u5bf9\u8bfb\u6bb5\uff0c\u8f93\u5165\u8bb0\u5f55\u6570\u91cf\u5747\u4e3a\u4e00\u5343\u3002\u7532\u3001\u4e59\u3001\u4e19\u7684\u5e73\u5747\u8986\u76d6\u5747\u4e3a\u4e94\u5341\uff0c\u8986\u76d6\u5e7f\u5ea6\u4e3a\u4e00\uff0c\u8fbe\u5230\u4f4d\u70b9\u8986\u76d6\u95e8\u69db\u7684\u6bd4\u4f8b\u4e5f\u4e3a\u4e00\u3002<\/p>\n
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\u672c\u6b21\u4f4e\u8d28\u91cf\u5bf9\u7167\u7684\u8bfb\u6bb5\u5bf9\u4e5f\u5168\u90e8\u4fdd\u7559\uff0c\u4f46\u67d0\u4e2a\u4f4d\u7f6e\u4e0a\u7684\u90e8\u5206\u78b1\u57fa\u6ca1\u6709\u8fdb\u5165\u4f4d\u70b9\u8ba1\u6570\u3002\u8fd9\u8bf4\u660e\u8bfb\u6bb5\u5c42\u9762\u901a\u8fc7\u8fc7\u6ee4\uff0c\u4e0e\u6bcf\u4e2a\u78b1\u57fa\u90fd\u80fd\u53c2\u4e0e\u53d8\u5f02\u8ba1\u7b97\uff0c\u662f\u4e24\u4e2a\u9700\u8981\u5206\u522b\u68c0\u67e5\u7684\u73af\u8282\u3002<\/p>\n
\u7532\u4e59\u4f18\u52bf\u78b1\u57fa\u4e0d\u540c\uff0c\u4e3a\u4ec0\u4e48 popANI \u4ecd\u4e3a\u4e00<\/h2>\n
\u7532\u7684\u76ee\u6807\u4f4d\u7f6e\u4ee5 T \u4e3a\u4e3b\uff0c\u5360\u767e\u5206\u4e4b\u516b\u5341\uff0cA \u5360\u767e\u5206\u4e4b\u4e8c\u5341\u3002\u4e59\u7684\u76ee\u6807\u4f4d\u7f6e\u4ee5 A \u4e3a\u4e3b\uff0c\u5360\u767e\u5206\u4e4b\u516b\u5341\uff0cT \u5360\u767e\u5206\u4e4b\u4e8c\u5341\u3002\u5b9e\u9645\u4f4d\u70b9\u8f93\u51fa\u5b8c\u6574\u4fdd\u5b58\u4e86\u8fd9\u4e24\u7ec4\u8ba1\u6570\u3002<\/p>\n
\u63a5\u7740\u8fd0\u884c\u6b63\u5e38\u6bd4\u8f83\u547d\u4ee4\uff0c\u5e76\u8981\u6c42\u4fdd\u5b58\u5dee\u5f02\u4f4d\u7f6e\uff0c\u4ee5\u4fbf\u4ece\u6c47\u603b\u7ed3\u679c\u9000\u56de\u5177\u4f53\u4f4d\u70b9\u3002<\/p>\n
inStrain compare \\\r\n  -i A.IS B.IS C.IS \\\r\n  -o compare_all -p 2 \\\r\n  -s genomes.stb \\\r\n  --skip_plot_generation \\\r\n  --store_mismatch_locations\r\n<\/code><\/pre>\n
\u7532\u4e59\u5b9e\u9645\u6bd4\u8f83\u4e86\u5168\u90e8\u4e24\u5343\u4e2a\u4f4d\u70b9\u3002\u76ee\u6807\u4f4d\u7f6e\u7684\u5171\u8bc6\u78b1\u57fa\u5206\u522b\u4e3a T \u548c A\uff0c\u56e0\u6b64\u5171\u8bc6\u5dee\u5f02\u8ba1\u6570\u4e3a\u4e00\uff0c\u5bf9\u5e94\u5171\u8bc6\u4e00\u81f4\u6027\u4e3a\u4e00\u51cf\u53bb\u4e24\u5343\u5206\u4e4b\u4e00\uff0c\u4e5f\u5c31\u662f\u96f6\u70b9\u4e5d\u4e5d\u4e5d\u4e94\u3002<\/p>\n
\u7fa4\u4f53\u6bd4\u8f83\u4e2d\uff0c\u7532\u548c\u4e59\u90fd\u4fdd\u7559\u4e86 T \u4e0e A\uff0c\u5b58\u5728\u5171\u4eab\u7684\u7b49\u4f4d\u57fa\u56e0\u3002\u56e0\u6b64\u8be5\u4f4d\u7f6e\u6ca1\u6709\u88ab\u8ba1\u4e3a\u7fa4\u4f53\u5dee\u5f02\uff0c\u7fa4\u4f53\u5dee\u5f02\u8ba1\u6570\u4e3a\u96f6\uff0c\u7fa4\u4f53\u4e00\u81f4\u6027\u4e3a\u4e00\u3002<\/p>\n
compared_bases_count = 2000\r\nconsensus_SNPs       = 1\r\npopulation_SNPs      = 0\r\nconANI              = 0.9995\r\npopANI              = 1.0\r\n<\/code><\/pre>\n
\u8fd9\u4e2a\u7ed3\u679c\u4e0e\u4e24\u7ec4\u8bfb\u6bb5\u7ec4\u6210\u4e0d\u540c\u540c\u65f6\u6210\u7acb\u3002\u7fa4\u4f53\u4e00\u81f4\u6027\u4e3a\u4e00\uff0c\u8bf4\u660e\u6309\u7167\u672c\u6b21\u53ef\u6bd4\u8f83\u4f4d\u7f6e\u548c\u68c0\u51fa\u6761\u4ef6\uff0c\u6ca1\u6709\u89c2\u5bdf\u5230\u4e0d\u5171\u4eab\u7b49\u4f4d\u57fa\u56e0\u7684\u5dee\u5f02\u3002\u5b83\u6ca1\u6709\u628a\u4e24\u4e2a\u6837\u672c\u7684\u7b49\u4f4d\u57fa\u56e0\u6bd4\u4f8b\u89c4\u5b9a\u4e3a\u76f8\u540c\u3002<\/p>\n
\u5982\u679c\u7814\u7a76\u95ee\u9898\u6d89\u53ca\u83cc\u682a\u6bd4\u4f8b\u53d8\u5316\uff0c\u53ef\u4ee5\u8fdb\u4e00\u6b65\u6bd4\u8f83\u4f4d\u70b9\u9891\u7387\u3001\u591a\u4e2a\u8fde\u9501\u4f4d\u70b9\u4ee5\u53ca\u5176\u4ed6\u8bc1\u636e\u3002\u5355\u4e2a\u4e00\u81f4\u6027\u6570\u503c\u4fdd\u7559\u7684\u4fe1\u606f\u6709\u9650\uff0c\u9700\u8981\u56de\u5230\u8bfb\u6bb5\u652f\u6301\u6765\u56de\u7b54\u6bd4\u4f8b\u662f\u5426\u6539\u53d8\u3002<\/p>\n
\u52a0\u5165\u5b8c\u5168\u4e0d\u540c\u7684\u7b49\u4f4d\u57fa\u56e0\u540e\uff0c\u6307\u6807\u5982\u4f55\u54cd\u5e94<\/h2>\n
\u4e19\u6837\u672c\u5728\u76ee\u6807\u4f4d\u7f6e\u53ea\u4fdd\u7559 C\uff0c\u4e0e\u7532\u4e59\u7684 T \u548c A \u90fd\u4e0d\u5171\u4eab\u3002\u5b9e\u9645\u4f4d\u70b9\u8f93\u51fa\u663e\u793a\u4e94\u5341\u6761 C\uff0c\u53c2\u8003\u78b1\u57fa T \u7684\u8ba1\u6570\u4e3a\u96f6\u3002\u8be5\u4f4d\u70b9\u88ab\u8bb0\u5f55\u4e3a\u76f8\u5bf9\u53c2\u8003\u7684\u56fa\u5b9a\u66ff\u6362\u3002<\/p>\n
\u7532\u4e0e\u4e19\u3001\u4e59\u4e0e\u4e19\u7684\u6bd4\u8f83\u90fd\u8986\u76d6\u5168\u90e8\u4e24\u5343\u4e2a\u4f4d\u70b9\u3002\u4e24\u7ec4\u6bd4\u8f83\u7684\u5171\u8bc6\u5dee\u5f02\u548c\u7fa4\u4f53\u5dee\u5f02\u5747\u4e3a\u4e00\uff0c\u56e0\u6b64\u4e24\u4e2a\u4e00\u81f4\u6027\u6307\u6807\u90fd\u7b49\u4e8e\u96f6\u70b9\u4e5d\u4e5d\u4e5d\u4e94\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u6bd4\u8f83<\/th>\n\u5b9e\u9645\u6bd4\u8f83\u4f4d\u7f6e\u6570<\/th>\n\u5171\u8bc6\u5dee\u5f02<\/th>\n\u7fa4\u4f53\u5dee\u5f02<\/th>\nconANI<\/th>\npopANI<\/th>\n<\/tr>\n<\/thead>\n
\u7532\u4e0e\u4e59<\/td>\n2000<\/td>\n1<\/td>\n0<\/td>\n0.9995<\/td>\n1<\/td>\n<\/tr>\n
\u7532\u4e0e\u4e19<\/td>\n2000<\/td>\n1<\/td>\n1<\/td>\n0.9995<\/td>\n0.9995<\/td>\n<\/tr>\n
\u4e59\u4e0e\u4e19<\/td>\n2000<\/td>\n1<\/td>\n1<\/td>\n0.9995<\/td>\n0.9995<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u4e09\u7ec4\u6bd4\u8f83\u7684\u5171\u8bc6\u4e00\u81f4\u6027\u76f8\u540c\uff0c\u7fa4\u4f53\u4e00\u81f4\u6027\u5374\u80fd\u533a\u5206\u7532\u4e59\u4e4b\u95f4\u5171\u4eab\u7b49\u4f4d\u57fa\u56e0\u7684\u60c5\u51b5\u3002\u8fd9\u6b63\u662f\u4fdd\u5b58\u4e24\u7c7b\u6307\u6807\u7684\u4ef7\u503c\u3002\u53ea\u4fdd\u7559\u5176\u4e2d\u4e00\u4e2a\u5b57\u6bb5\uff0c\u4f1a\u5931\u53bb\u53e6\u4e00\u5c42\u53ef\u4ee5\u89e3\u91ca\u7fa4\u4f53\u7ec4\u6210\u7684\u4fe1\u606f\u3002<\/p>\n
\u672c\u6b21\u7a0b\u5e8f\u7684\u805a\u7c7b\u8f93\u51fa\u628a\u7532\u4e59\u653e\u5728\u540c\u4e00\u7ec4\uff0c\u4e19\u653e\u5728\u53e6\u4e00\u7ec4\u3002\u8fd9\u4e2a\u7ed3\u679c\u53ef\u4ee5\u4f5c\u4e3a\u8f6f\u4ef6\u5728\u6307\u5b9a\u6761\u4ef6\u4e0b\u7684\u5206\u7c7b\u8bb0\u5f55\uff0c\u4f46\u6559\u5b66\u53c2\u8003\u53ea\u6709\u4e24\u5343\u78b1\u57fa\uff0c\u4e0d\u80fd\u636e\u6b64\u7ed9\u771f\u5b9e\u9879\u76ee\u8bbe\u5b9a\u666e\u904d\u9002\u7528\u7684\u83cc\u682a\u5224\u5b9a\u754c\u9650\u3002<\/p>\n
\u78b1\u57fa\u8d28\u91cf\u4e3a\u4ec0\u4e48\u4f1a\u8ba9\u4e00\u4e2a\u53d8\u5f02\u6d88\u5931<\/h2>\n
\u4f4e\u8d28\u91cf\u5bf9\u7167\u4e0e\u7532\u62e5\u6709\u76f8\u540c\u7684\u8bfb\u6bb5\u5e8f\u5217\uff0c\u53ea\u628a\u5341\u6761 A \u5728\u76ee\u6807\u4f4d\u7f6e\u7684\u8d28\u91cf\u964d\u4f4e\u5230\u4e8c\u5341\u3002\u9ed8\u8ba4\u8d28\u91cf\u95e8\u69db\u4e3a\u4e09\u5341\uff0c\u8fd9\u4e9b\u78b1\u57fa\u4e0d\u518d\u8ba1\u5165\u8be5\u4f4d\u7f6e\u7684\u53d8\u5f02\u652f\u6301\uff0c\u56db\u5341\u6761\u9ad8\u8d28\u91cf T \u4ecd\u7136\u4fdd\u7559\u3002<\/p>\n
\u5b9e\u9645\u8f93\u51fa\u4e2d\uff0c\u4f4e\u8d28\u91cf\u5bf9\u7167\u7684\u53d8\u5f02\u8868\u4e3a\u7a7a\uff0c\u53d8\u5f02\u4f4d\u70b9\u8ba1\u6570\u4e3a\u96f6\u3002\u5e73\u5747\u8986\u76d6\u7531\u4e94\u5341\u964d\u5230\u56db\u5341\u4e5d\u70b9\u4e5d\u4e5d\u4e94\uff0c\u6070\u597d\u76f8\u5f53\u4e8e\u5168\u957f\u7d2f\u8ba1\u8986\u76d6\u51cf\u5c11\u5341\u4e2a\u78b1\u57fa\uff0c\u518d\u9664\u4ee5\u4e24\u5343\u3002<\/p>\n
\u539f\u6709\u7d2f\u8ba1\u8986\u76d6 = 50 \u00d7 2000 = 100000\r\n\u51cf\u5c11\u7684\u6709\u6548\u78b1\u57fa = 10\r\n\u65b0\u7684\u5e73\u5747\u8986\u76d6 = 99990 \/ 2000 = 49.995\r\n<\/code><\/pre>\n
\u5c3d\u7ba1\u5168\u5c40\u5e73\u5747\u8986\u76d6\u53ea\u4e0b\u964d\u4e86\u96f6\u70b9\u96f6\u96f6\u4e94\uff0c\u76ee\u6807\u4f4d\u7f6e\u7684\u6b21\u8981\u7b49\u4f4d\u57fa\u56e0\u5df2\u7ecf\u5b8c\u5168\u5931\u53bb\u6709\u6548\u652f\u6301\u3002\u56e0\u6b64\uff0c\u5bf9\u4e8e\u5173\u5fc3\u7684\u53d8\u5f02\uff0c\u5e94\u8be5\u68c0\u67e5\u4f4d\u70b9\u8d28\u91cf\u548c\u78b1\u57fa\u8ba1\u6570\uff0c\u5355\u770b\u5168\u957f\u5e73\u5747\u8986\u76d6\u53ef\u80fd\u770b\u4e0d\u51fa\u5f71\u54cd\u3002<\/p>\n
\u7532\u4e0e\u4f4e\u8d28\u91cf\u5bf9\u7167\u7684\u4e24\u4e2a\u4e00\u81f4\u6027\u6307\u6807\u90fd\u4e3a\u4e00\uff0c\u56e0\u4e3a\u5b83\u4eec\u7684\u5171\u8bc6\u4ecd\u662f T\uff0c\u7fa4\u4f53\u6bd4\u8f83\u4e5f\u5171\u4eab T\u3002\u4f46\u7532\u4fdd\u7559\u4e00\u4e2a\u591a\u6001\u4f4d\u70b9\uff0c\u4f4e\u8d28\u91cf\u5bf9\u7167\u6ca1\u6709\u68c0\u51fa\u8be5\u4f4d\u70b9\u7684\u591a\u6001\u6027\u3002\u518d\u6b21\u8bf4\u660e\uff0c\u4e0d\u540c\u8f93\u51fa\u5b57\u6bb5\u5173\u6ce8\u7684\u662f\u4e0d\u540c\u5c42\u9762\u7684\u95ee\u9898\u3002<\/p>\n
\u8c03\u6574\u8d28\u91cf\u95e8\u69db\u65f6\uff0c\u8fd8\u5e94\u540c\u65f6\u8003\u8651\u5bf9\u5e94\u9519\u8bef\u6a21\u578b\u548c\u6d4b\u5e8f\u5e73\u53f0\u7684\u8d28\u91cf\u542b\u4e49\u3002\u628a\u95e8\u69db\u8c03\u4f4e\u80fd\u591f\u7eb3\u5165\u66f4\u591a\u78b1\u57fa\uff0c\u4e5f\u6539\u53d8\u4e86\u5982\u4f55\u533a\u5206\u771f\u5b9e\u53d8\u5f02\u4e0e\u9519\u8bef\u7684\u6761\u4ef6\uff0c\u9700\u8981\u7ed3\u5408\u5b9e\u9645\u6570\u636e\u8fdb\u884c\u5224\u65ad\u3002<\/p>\n
\u591a\u6837\u6027\u6570\u503c\u4e5f\u80fd\u4ece\u672c\u4f8b\u590d\u7b97<\/h2>\n
\u7532\u4e59\u7684\u76ee\u6807\u4f4d\u7f6e\u90fd\u5b58\u5728\u767e\u5206\u4e4b\u516b\u5341\u4e0e\u767e\u5206\u4e4b\u4e8c\u5341\u4e24\u79cd\u78b1\u57fa\u3002\u7528\u4e00\u51cf\u53bb\u5404\u78b1\u57fa\u9891\u7387\u5e73\u65b9\u548c\u8ba1\u7b97\u8be5\u4f4d\u70b9\u7684\u591a\u6837\u6027\uff0c\u5f97\u5230\u96f6\u70b9\u4e09\u4e8c\u3002\u5176\u4ed6\u4e00\u5343\u4e5d\u767e\u4e5d\u5341\u4e5d\u4e2a\u4f4d\u70b9\u53ea\u6709\u4e00\u79cd\u78b1\u57fa\uff0c\u8d21\u732e\u4e3a\u96f6\u3002<\/p>\n
\u5c06\u8fd9\u4e2a\u4f4d\u70b9\u7684\u8d21\u732e\u5206\u644a\u5230\u5168\u90e8\u4e24\u5343\u4e2a\u8986\u76d6\u4f4d\u7f6e\uff0c\u5f97\u5230\u96f6\u70b9\u96f6\u96f6\u96f6\u4e00\u516d\u3002\u7a0b\u5e8f\u4e3a\u7532\u4e59\u62a5\u544a\u7684\u666e\u901a\u6838\u82f7\u9178\u591a\u6837\u6027\u90fd\u63a5\u8fd1\u8fd9\u4e00\u6570\u503c\uff0c\u6d6e\u70b9\u8868\u793a\u5bfc\u81f4\u672b\u4f4d\u7565\u6709\u5dee\u522b\u3002<\/p>\n
\u4e19\u53ea\u6709\u5355\u4e00 C\uff0c\u867d\u7136\u76f8\u5bf9\u53c2\u8003\u53d1\u751f\u56fa\u5b9a\u66ff\u6362\uff0c\u7fa4\u4f53\u5185\u90e8\u5728\u8fd9\u4e2a\u4f4d\u7f6e\u4ecd\u7136\u53ea\u6709\u4e00\u79cd\u78b1\u57fa\uff0c\u56e0\u6b64\u672c\u4f8b\u7684\u6838\u82f7\u9178\u591a\u6837\u6027\u4e3a\u96f6\u3002\u56fa\u5b9a\u66ff\u6362\u6570\u91cf\u4e0e\u7fa4\u4f53\u5185\u90e8\u591a\u6001\u7a0b\u5ea6\u53ef\u4ee5\u53d1\u751f\u4e0d\u540c\u53d8\u5316\uff0c\u89e3\u91ca\u65f6\u5e94\u5206\u522b\u67e5\u770b\u3002<\/p>\n
\u7ed3\u679c\u8fd8\u5305\u542b\u7ecf\u8fc7\u62bd\u6837\u5904\u7406\u7684\u591a\u6837\u6027\u5b57\u6bb5\u3002\u8fd9\u4e9b\u5b57\u6bb5\u6d89\u53ca\u989d\u5916\u968f\u673a\u8fc7\u7a0b\uff0c\u590d\u73b0\u65f6\u53ef\u80fd\u51fa\u73b0\u5c0f\u5e45\u53d8\u5316\u3002\u6bd4\u8f83\u672c\u4f8b\u7684\u786e\u5b9a\u6027\u7ed3\u8bba\uff0c\u5e94\u9996\u5148\u6838\u5bf9\u666e\u901a\u591a\u6837\u6027\u3001\u5b9e\u9645\u4f4d\u70b9\u8ba1\u6570\u548c\u53ef\u6bd4\u8f83\u4f4d\u7f6e\uff0c\u518d\u5355\u72ec\u89e3\u91ca\u62bd\u6837\u6307\u6807\u3002<\/p>\n
\u5728\u961f\u5217\u5206\u6790\u4e2d\uff0c\u53ef\u4ee5\u4e3a\u6bcf\u4e00\u5bf9\u6837\u672c\u5efa\u7acb\u4e00\u884c\u6838\u5bf9\u8bb0\u5f55\uff0c\u540c\u65f6\u4fdd\u5b58\u53c2\u8003\u7248\u672c\u3001\u6709\u6548\u6bd4\u8f83\u4f4d\u7f6e\u6570\u3001\u8986\u76d6\u6bd4\u4f8b\u3001\u4e24\u7c7b\u5dee\u5f02\u8ba1\u6570\u548c\u4e24\u4e2a\u4e00\u81f4\u6027\u6307\u6807\u3002\u8fd9\u6837\u5728\u67d0\u4e00\u5bf9\u6837\u672c\u7a81\u7136\u8868\u73b0\u5f97\u7279\u522b\u63a5\u8fd1\u65f6\uff0c\u53ef\u4ee5\u5148\u786e\u8ba4\u5b83\u4eec\u662f\u5426\u53ea\u662f\u5171\u540c\u8986\u76d6\u533a\u57df\u8f83\u5c11\uff0c\u518d\u68c0\u67e5\u4f4d\u70b9\u5c42\u9762\u7684\u8bc1\u636e\u3002<\/p>\n
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