{"id":72757,"date":"2026-11-15T10:34:51","date_gmt":"2026-11-15T02:34:51","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72757"},"modified":"2026-11-15T10:34:51","modified_gmt":"2026-11-15T02:34:51","slug":"qiime2-feature-filter-rarefaction-sample-loss-provenance","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/qiime2-feature-filter-rarefaction-sample-loss-provenance\/","title":{"rendered":"QIIME 2 \u62bd\u5e73\u540e\u6837\u672c\u4e3a\u4ec0\u4e48\u5c11\u4e86\uff1f\u5b9e\u8dd1\u8ba1\u6570\u5bfc\u5165\u3001\u7279\u5f81\u8fc7\u6ee4\u3001\u62bd\u6837\u4e0e\u6765\u6e90\u8ffd\u8e2a"},"content":{"rendered":"
\n

\u6269\u589e\u5b50\u5206\u6790\u8fdb\u5165\u591a\u6837\u6027\u8ba1\u7b97\u4e4b\u524d\uff0c\u5e38\u5e38\u9700\u8981\u68c0\u67e5\u7279\u5f81\u8868\u548c\u6837\u672c\u6d4b\u5e8f\u91cf\u3002\u4e00\u4e2a\u5bb9\u6613\u4ee4\u4eba\u56f0\u60d1\u7684\u60c5\u51b5\u662f\uff0c\u539f\u6765\u6709\u4e09\u4e2a\u6837\u672c\uff0c\u7ecf\u8fc7\u8fc7\u6ee4\u548c\u62bd\u5e73\u4e4b\u540e\u53ea\u5269\u4e00\u4e2a\uff0c\u8fd0\u884c\u65e5\u5fd7\u5374\u6ca1\u6709\u62a5\u9519\u3002\u8981\u89e3\u91ca\u8fd9\u79cd\u53d8\u5316\uff0c\u9700\u8981\u628a\u6bcf\u4e00\u6b65\u4f7f\u7528\u7684\u8ba1\u6570\u8868\u548c\u4fdd\u7559\u6761\u4ef6\u8fde\u63a5\u8d77\u6765\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u5b89\u88c5 QIIME 2 \u7684 2026.10 \u5b98\u65b9\u53d1\u884c\u73af\u5883\uff0c\u4ece\u4e00\u5f20\u56db\u4e2a\u7279\u5f81\u3001\u4e09\u4e2a\u6837\u672c\u7684\u4eba\u5de5\u8ba1\u6570\u8868\u5f00\u59cb\uff0c\u8fd0\u884c\u5bfc\u5165\u3001\u6c47\u603b\u3001\u8fc7\u6ee4\u3001\u56fa\u5b9a\u79cd\u5b50\u62bd\u5e73\u3001\u76f8\u5bf9\u9891\u7387\u8f6c\u6362\u53ca\u5bfc\u51fa\u6838\u5bf9\u3002\u5b9e\u9a8c\u540c\u65f6\u68c0\u67e5\u6570\u636e\u7c7b\u578b\u4e0e\u6765\u6e90\u8bb0\u5f55\uff0c\u8bfb\u8005\u53ef\u4ee5\u7528\u5f88\u5c0f\u7684\u6570\u636e\u7406\u89e3\u5b8c\u6574\u7684\u8868\u683c\u5904\u7406\u8fc7\u7a0b\u3002<\/p>\n

\"\u62bd\u5e73\u540e\uff0c\u6837\u672c\u4e3a\u4ec0\u4e48\u5c11\u4e86\u3002QIIME
QIIME 2 2026.10 \u5bfc\u5165\u3001\u8fc7\u6ee4\u4e0e\u62bd\u6837\u5b9e\u8dd1<\/figcaption><\/figure>\n

\u5148\u786e\u8ba4\u624b\u91cc\u7684\u662f\u54ea\u4e00\u79cd\u8868<\/h2>\n

\u7279\u5f81\u8ba1\u6570\u8868\u8bb0\u5f55\u6bcf\u4e2a\u6837\u672c\u4e2d\u5404\u4e2a\u7279\u5f81\u51fa\u73b0\u591a\u5c11\u6b21\u3002\u76f8\u5bf9\u9891\u7387\u8868\u5219\u628a\u6bcf\u5217\u9664\u4ee5\u8be5\u6837\u672c\u7684\u603b\u8ba1\u6570\uff0c\u4f7f\u6bcf\u4e2a\u6837\u672c\u7684\u5408\u8ba1\u53d8\u4e3a\u4e00\u3002\u4e24\u8005\u53ef\u80fd\u91c7\u7528\u7c7b\u4f3c\u6587\u4ef6\u683c\u5f0f\uff0c\u4f46\u6570\u503c\u542b\u4e49\u4e0d\u540c\u3002<\/p>\n

QIIME 2 \u7684\u4ea7\u7269\u4f1a\u8bb0\u5f55\u76f8\u5e94\u7c7b\u578b\u3002\u8ba1\u6570\u8868\u4f7f\u7528 FeatureTable[Frequency]<\/code>\uff0c\u76f8\u5bf9\u9891\u7387\u4f7f\u7528 FeatureTable[RelativeFrequency]<\/code>\u3002\u5bfc\u5165\u65f6\u6b63\u786e\u58f0\u660e\u7c7b\u578b\uff0c\u540e\u7eed\u547d\u4ee4\u624d\u80fd\u4f9d\u636e\u6570\u636e\u542b\u4e49\u68c0\u67e5\u662f\u5426\u63a5\u53d7\u8f93\u5165\u3002\u5b98\u65b9\u7279\u5f81\u8868\u5bfc\u5165\u8bf4\u660e<\/a><\/p>\n

\u5f00\u59cb\u5206\u6790\u524d\uff0c\u53ef\u4ee5\u5148\u68c0\u67e5\u6700\u57fa\u672c\u7684\u4e09\u4e2a\u95ee\u9898\u3002\u884c\u662f\u5426\u4ee3\u8868\u7279\u5f81\uff0c\u5217\u662f\u5426\u4ee3\u8868\u6837\u672c\uff0c\u6570\u503c\u662f\u539f\u59cb\u8ba1\u6570\u8fd8\u662f\u5df2\u7ecf\u5f52\u4e00\u5316\u3002\u5c24\u5176\u662f\u4ece\u5176\u4ed6\u8f6f\u4ef6\u5bfc\u51fa\u7684\u8868\u683c\uff0c\u6587\u4ef6\u540d\u4e2d\u5199\u7740\u4e30\u5ea6\uff0c\u5e76\u4e0d\u80fd\u5b8c\u6574\u8bf4\u660e\u8fd9\u4e9b\u95ee\u9898\u3002<\/p>\n

\u672c\u6b21\u56db\u4e2a\u884c\u540d\u53ea\u662f\u6559\u5b66\u6807\u7b7e\uff0c\u6ca1\u6709\u5bf9\u5e94\u771f\u5b9e\u6269\u589e\u5b50\u5e8f\u5217\u3002\u6211\u4eec\u4ece\u8ba1\u6570\u8868\u5f00\u59cb\uff0c\u56e0\u6b64\u5b9e\u9a8c\u8303\u56f4\u4e0d\u5305\u542b\u8bfb\u6bb5\u8d28\u63a7\u3001\u53bb\u566a\u6216\u5206\u7c7b\uff0c\u4e5f\u4e0d\u628a\u8fd9\u4e9b\u6807\u7b7e\u5f53\u4f5c\u5df2\u7ecf\u9a8c\u8bc1\u7684\u751f\u7269\u5b66\u7279\u5f81\u3002<\/p>\n

\u4e09\u4e2a\u6837\u672c\u7684\u8f93\u5165\u8ba1\u6570\u600e\u6837\u5b89\u6392<\/h2>\n

\u7532\u6837\u672c\u7684\u603b\u8ba1\u6570\u4e3a\u4e00\u767e\uff0c\u4e59\u4e3a\u5341\uff0c\u4e19\u4e3a\u5341\u4e8c\u3002\u7b2c\u56db\u4e2a\u7279\u5f81\u53ea\u5728\u4e19\u4e2d\u51fa\u73b0\u4e24\u6b21\uff0c\u5176\u4ed6\u4e09\u4e2a\u7279\u5f81\u5206\u522b\u51fa\u73b0\u5728\u81f3\u5c11\u4e24\u4e2a\u6837\u672c\u4e2d\u3002\u8fd9\u4e2a\u5b89\u6392\u8ba9\u7279\u5f81\u8fc7\u6ee4\u4e0e\u6837\u672c\u603b\u91cf\u4e4b\u95f4\u7684\u5173\u7cfb\u80fd\u591f\u76f4\u63a5\u8ba1\u7b97\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n\n
\u7279\u5f81<\/th>\nSample_A<\/th>\nSample_B<\/th>\nSample_C<\/th>\n\u51fa\u73b0\u7684\u6837\u672c\u6570<\/th>\n<\/tr>\n<\/thead>\n
ASV1<\/td>\n80<\/td>\n8<\/td>\n0<\/td>\n2<\/td>\n<\/tr>\n
ASV2<\/td>\n15<\/td>\n2<\/td>\n5<\/td>\n3<\/td>\n<\/tr>\n
ASV3<\/td>\n5<\/td>\n0<\/td>\n5<\/td>\n2<\/td>\n<\/tr>\n
ASV4<\/td>\n0<\/td>\n0<\/td>\n2<\/td>\n1<\/td>\n<\/tr>\n
\u603b\u8ba1\u6570<\/td>\n100<\/td>\n10<\/td>\n12<\/td>\n\u4e0d\u9002\u7528<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u4e0b\u8f7d\u5305\u4e2d\u7684\u811a\u672c\u4f7f\u7528 BIOM \u5e93\u751f\u6210\u6587\u4ef6\uff0c\u5c06\u884c\u5217\u540d\u79f0\u4e0e\u6570\u503c\u4e00\u540c\u4fdd\u5b58\u3002\u8bfb\u8005\u4e5f\u53ef\u4ee5\u5148\u5728\u7eb8\u4e0a\u8ba1\u7b97\u6bcf\u5217\u603b\u548c\uff0c\u518d\u4e0e\u5bfc\u5165\u540e\u7684\u7ed3\u679c\u6838\u5bf9\u3002\u5bf9\u4e8e\u771f\u5b9e\u8868\u683c\uff0c\u8fd9\u79cd\u6838\u5bf9\u6709\u52a9\u4e8e\u53d1\u73b0\u8f6c\u7f6e\u3001\u9057\u6f0f\u6837\u672c\u548c\u9519\u8bef\u5f52\u4e00\u5316\u7b49\u95ee\u9898\u3002<\/p>\n

\u8fd9\u91cc\u91c7\u7528\u5341\u548c\u5341\u4e00\u4e24\u4e2a\u5f88\u5c0f\u7684\u62bd\u6837\u6df1\u5ea6\uff0c\u53ea\u4e3a\u8bf4\u660e\u7a0b\u5e8f\u884c\u4e3a\u3002\u6b63\u5f0f\u6269\u589e\u5b50\u9879\u76ee\u9700\u8981\u6839\u636e\u5b9e\u9645\u4fdd\u7559\u8bfb\u6bb5\u3001\u5206\u6790\u76ee\u6807\u548c\u6837\u672c\u5206\u5e03\u51b3\u5b9a\u6df1\u5ea6\uff0c\u4e0d\u80fd\u76f4\u63a5\u6cbf\u7528\u672c\u4f8b\u7684\u6559\u5b66\u6570\u503c\u3002<\/p>\n

\u5148\u56fa\u5b9a\u53d1\u884c\u73af\u5883\uff0c\u518d\u6267\u884c\u5bfc\u5165<\/h2>\n

\u672c\u6b21\u4f7f\u7528\u5b98\u65b9\u7684 quay.io\/qiime2\/qiime2:2026.10<\/code> \u955c\u50cf\u3002\u5b9e\u9645\u73af\u5883\u663e\u793a Python 3.12.16\uff0c\u6846\u67b6\u7248\u672c\u4e3a rachis 2026.10.1\uff0c\u547d\u4ee4\u884c\u5305\u4e3a rachis-cli 2026.10.0\uff0c\u7279\u5f81\u8868\u63d2\u4ef6\u4e3a 2026.10.0\u3002\u955c\u50cf\u6458\u8981\u8bb0\u5f55\u5728\u4e0b\u8f7d\u5305\u4e2d\u3002\u5b98\u65b9\u5b89\u88c5\u8bf4\u660e<\/a><\/p>\n

\u547d\u4ee4\u540d\u79f0\u4ecd\u7136\u53ef\u4ee5\u4f7f\u7528 qiime<\/code>\u3002\u590d\u73b0\u65f6\u5148\u8fd0\u884c\u73af\u5883\u4fe1\u606f\u547d\u4ee4\uff0c\u518d\u67e5\u770b\u5177\u4f53\u63d2\u4ef6\u7684\u5e2e\u52a9\uff0c\u53ef\u4ee5\u786e\u8ba4\u624b\u5934\u7248\u672c\u63d0\u4f9b\u54ea\u4e9b\u8f93\u5165\u3001\u8f93\u51fa\u548c\u53c2\u6570\u3002<\/p>\n

qiime info\r\n\r\nqiime tools import \\\r\n  --input-path counts.biom \\\r\n  --type 'FeatureTable[Frequency]' \\\r\n  --input-format BIOMV210Format \\\r\n  --output-path counts.qza\r\n<\/code><\/pre>\n
\u8f93\u5165\u683c\u5f0f\u63cf\u8ff0\u6587\u4ef6\u5982\u4f55\u5b58\u50a8\uff0c\u4ea7\u7269\u7c7b\u578b\u63cf\u8ff0\u6570\u636e\u4ee3\u8868\u4ec0\u4e48\u3002\u8fd9\u91cc\u7684\u6587\u4ef6\u91c7\u7528 BIOM \u7684\u76f8\u5e94\u5b58\u50a8\u683c\u5f0f\uff0c\u6570\u503c\u542b\u4e49\u662f\u8ba1\u6570\uff0c\u4e24\u9879\u90fd\u4e0e\u5b9e\u9645\u8f93\u5165\u4e00\u81f4\u3002<\/p>\n
\u5bfc\u5165\u4ee5\u540e\uff0c\u6211\u4eec\u8fd8\u4f1a\u628a\u4ea7\u7269\u5bfc\u51fa\u5e76\u91cd\u65b0\u8bfb\u53d6\u8ba1\u6570\u77e9\u9635\u3002\u8fd9\u6837\u53ef\u4ee5\u540c\u65f6\u6838\u5bf9\u6837\u672c\u540d\u79f0\u3001\u7279\u5f81\u540d\u79f0\u548c\u6570\u503c\uff0c\u786e\u8ba4\u540e\u7eed\u6b65\u9aa4\u662f\u5728\u9884\u671f\u8f93\u5165\u4e0a\u8fd0\u884c\u3002\u53ea\u770b\u6587\u4ef6\u5b58\u5728\u6216\u547d\u4ee4\u8fd4\u56de\u6210\u529f\uff0c\u65e0\u6cd5\u5b8c\u6210\u8fd9\u4e9b\u5185\u5bb9\u5c42\u9762\u7684\u68c0\u67e5\u3002<\/p>\n
\u6c47\u603b\u7ed3\u679c\u600e\u6837\u5e2e\u52a9\u9009\u62e9\u4e0b\u4e00\u6b65<\/h2>\n
\u672c\u6b21\u7248\u672c\u7684\u6c47\u603b\u547d\u4ee4\u751f\u6210\u4e09\u4e2a\u8f93\u51fa\uff0c\u5206\u522b\u4fdd\u5b58\u7279\u5f81\u9891\u6570\u3001\u6837\u672c\u9891\u6570\u4ee5\u53ca\u53ef\u89c6\u5316\u7ed3\u679c\u3002\u8fd0\u884c\u65f6\u660e\u786e\u5199\u51fa\u5404\u4e2a\u6587\u4ef6\uff0c\u4fbf\u4e8e\u540e\u7eed\u6309\u7528\u9014\u67e5\u627e\u3002<\/p>\n
qiime feature-table summarize \\\r\n  --i-table counts.qza \\\r\n  --o-feature-frequencies feature-frequencies.qza \\\r\n  --o-sample-frequencies sample-frequencies.qza \\\r\n  --o-summary summary.qzv\r\n<\/code><\/pre>\n
\u6837\u672c\u9891\u6570\u53ef\u4ee5\u5e2e\u52a9\u786e\u8ba4\u603b\u8ba1\u6570\u5206\u5e03\uff0c\u7279\u5f81\u9891\u6570\u53ef\u4ee5\u5e2e\u52a9\u68c0\u67e5\u7279\u5f81\u51fa\u73b0\u8303\u56f4\uff0c\u53ef\u89c6\u5316\u6587\u4ef6\u7528\u4e8e\u6d4f\u89c8\u6c47\u603b\u5185\u5bb9\u3002\u914d\u5408\u539f\u59cb\u77e9\u9635\u67e5\u770b\uff0c\u4f1a\u66f4\u5bb9\u6613\u7406\u89e3\u54ea\u4e9b\u6837\u672c\u63a5\u8fd1\u540e\u7eed\u95e8\u69db\u3002<\/p>\n
\u5728\u81ea\u5df1\u7684\u9879\u76ee\u4e2d\uff0c\u53ef\u4ee5\u5148\u5217\u51fa\u5019\u9009\u62bd\u6837\u6df1\u5ea6\uff0c\u4ee5\u53ca\u6bcf\u4e2a\u6df1\u5ea6\u4f1a\u4fdd\u7559\u591a\u5c11\u6837\u672c\uff0c\u518d\u628a\u6837\u672c\u5206\u7ec4\u4fe1\u606f\u63a5\u5165\u8fd9\u5f20\u8868\u3002\u5982\u679c\u4e22\u5931\u7684\u6837\u672c\u96c6\u4e2d\u5728\u67d0\u4e2a\u7ec4\uff0c\u540e\u7eed\u6bd4\u8f83\u7684\u6570\u636e\u7ec4\u6210\u5c31\u4f1a\u6539\u53d8\uff0c\u9700\u8981\u5728\u5206\u6790\u8bbe\u8ba1\u4e2d\u660e\u786e\u5904\u7406\u3002<\/p>\n
\u672c\u6b21\u8868\u683c\u89c4\u6a21\u5f88\u5c0f\uff0c\u5341\u548c\u5341\u4e00\u4e4b\u95f4\u53ea\u5dee\u4e00\u4e2a\u8ba1\u6570\uff0c\u5374\u8db3\u4ee5\u6539\u53d8\u4e59\u6837\u672c\u662f\u5426\u4fdd\u7559\u3002\u56e0\u6b64\uff0c\u5728\u62a5\u544a\u6df1\u5ea6\u65f6\u7ed9\u51fa\u5b8c\u6574\u6570\u503c\uff0c\u5e76\u4fdd\u5b58\u5b9e\u9645\u4fdd\u7559\u6837\u672c\u5217\u8868\uff0c\u6bd4\u53ea\u5199\u201c\u5927\u7ea6\u76f8\u540c\u7684\u6d4b\u5e8f\u91cf\u201d\u66f4\u4fbf\u4e8e\u590d\u67e5\u3002<\/p>\n
\u7b2c\u4e00\u7ec4\u62bd\u5e73\uff0c\u6df1\u5ea6\u4e3a\u5341\u65f6\u4e09\u4e2a\u6837\u672c\u90fd\u4fdd\u7559<\/h2>\n
\u62bd\u5e73\u4ece\u5404\u4e2a\u6837\u672c\u7684\u8ba1\u6570\u4e2d\u62bd\u53d6\u6307\u5b9a\u603b\u91cf\u3002\u672c\u6b21\u91c7\u7528\u9ed8\u8ba4\u7684\u4e0d\u653e\u56de\u65b9\u5f0f\uff0c\u5e76\u663e\u5f0f\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u3002\u6837\u672c\u9700\u8981\u62e5\u6709\u8db3\u591f\u8ba1\u6570\u624d\u80fd\u8fbe\u5230\u6240\u8bbe\u6df1\u5ea6\u3002<\/p>\n
qiime feature-table rarefy \\\r\n  --i-table counts.qza \\\r\n  --p-sampling-depth 10 \\\r\n  --p-random-seed 121 \\\r\n  --o-rarefied-table raw10.qza\r\n<\/code><\/pre>\n
\u5b9e\u9645\u8f93\u51fa\u4fdd\u7559\u7532\u3001\u4e59\u3001\u4e19\u4e09\u4e2a\u6837\u672c\uff0c\u6bcf\u4e2a\u6837\u672c\u5408\u8ba1\u5341\u3002\u4e59\u539f\u6765\u6b63\u597d\u6709\u5341\u4e2a\u8ba1\u6570\uff0c\u8fbe\u5230\u95e8\u69db\uff0c\u56e0\u6b64\u4fdd\u7559\u3002\u7532\u548c\u4e19\u7684\u8ba1\u6570\u603b\u91cf\u9ad8\u4e8e\u5341\uff0c\u8f93\u51fa\u5219\u662f\u62bd\u53d6\u540e\u7684\u7ec4\u6210\u3002<\/p>\n
\u8fd9\u91cc\u5e94\u533a\u5206\u4e24\u4e2a\u68c0\u67e5\u95ee\u9898\u3002\u603b\u8ba1\u6570\u662f\u5426\u7b49\u4e8e\u6307\u5b9a\u6df1\u5ea6\uff0c\u53ef\u4ee5\u6309\u5217\u6c42\u548c\u786e\u8ba4\u3002\u6bcf\u4e2a\u7279\u5f81\u62bd\u5230\u591a\u5c11\uff0c\u5219\u4e0e\u8f93\u5165\u7ec4\u6210\u548c\u968f\u673a\u62bd\u6837\u6709\u5173\uff0c\u9700\u8981\u8bfb\u53d6\u5b9e\u9645\u77e9\u9635\u3002\u603b\u91cf\u76f8\u540c\u7684\u4e24\u4e2a\u7ed3\u679c\uff0c\u5177\u4f53\u7279\u5f81\u8ba1\u6570\u4ecd\u7136\u53ef\u80fd\u4e0d\u540c\u3002<\/p>\n
\u672c\u6b21\u901a\u8fc7\u6b63\u5e38\u5bfc\u51fa\u547d\u4ee4\u5f97\u5230 BIOM \u6587\u4ef6\uff0c\u518d\u72ec\u7acb\u8bfb\u53d6\u6bcf\u4e2a\u6837\u672c\u7684\u603b\u8ba1\u6570\u3002\u8fd9\u4e2a\u6838\u5bf9\u8fc7\u7a0b\u53ef\u4ee5\u7528\u5728\u540e\u7eed\u4efb\u4f55\u8868\u683c\u6b65\u9aa4\u4e2d\uff0c\u5e2e\u52a9\u786e\u8ba4\u8f93\u51fa\u6570\u503c\u7b26\u5408\u9884\u671f\u3002<\/p>\n
\u7b2c\u4e8c\u7ec4\u628a\u6df1\u5ea6\u63d0\u9ad8\u5230\u5341\u4e00\uff0c\u4e59\u4e3a\u4f55\u6d88\u5931<\/h2>\n
\u7b2c\u4e8c\u7ec4\u76f4\u63a5\u4f7f\u7528\u540c\u4e00\u4efd\u539f\u59cb\u8ba1\u6570\u4ea7\u7269\uff0c\u5c06\u6df1\u5ea6\u6539\u4e3a\u5341\u4e00\uff0c\u5176\u4ed6\u6761\u4ef6\u4fdd\u6301\u4e00\u81f4\u3002\u7532\u6709\u4e00\u767e\u3001\u4e19\u6709\u5341\u4e8c\uff0c\u80fd\u591f\u8fbe\u5230\u95e8\u69db\uff0c\u4e59\u53ea\u6709\u5341\uff0c\u65e0\u6cd5\u4ece\u73b0\u6709\u8ba1\u6570\u4e2d\u62bd\u51fa\u5341\u4e00\u4e2a\u4e0d\u540c\u8ba1\u6570\u5355\u4f4d\u3002<\/p>\n
\u5b9e\u9645\u8f93\u51fa\u53ea\u4fdd\u7559\u7532\u548c\u4e19\uff0c\u4e24\u5217\u603b\u8ba1\u6570\u5747\u4e3a\u5341\u4e00\u3002\u4e59\u6ca1\u6709\u51fa\u73b0\u5728\u7ed3\u679c\u6837\u672c\u5217\u8868\u4e2d\uff0c\u8fd9\u662f\u5f53\u524d\u6761\u4ef6\u4e0b\u7684\u6b63\u5e38\u884c\u4e3a\u3002\u7279\u5f81\u8868\u63d2\u4ef6\u4e0e\u62bd\u5e73\u53c2\u6570\u8bf4\u660e<\/a><\/p>\n
\u56e0\u6b64\uff0c\u5982\u679c\u67d0\u4e2a\u6837\u672c\u5728\u62bd\u5e73\u540e\u6d88\u5931\uff0c\u53ef\u4ee5\u5148\u6838\u5bf9\u62bd\u5e73\u547d\u4ee4\u5b9e\u9645\u63a5\u6536\u7684\u8f93\u5165\u8868\uff0c\u518d\u8ba1\u7b97\u8be5\u6837\u672c\u5728\u90a3\u5f20\u8868\u4e2d\u7684\u603b\u91cf\u3002\u5373\u4f7f\u65e9\u671f\u6d4b\u5e8f\u7edf\u8ba1\u663e\u793a\u8bfb\u6bb5\u8f83\u591a\uff0c\u7ecf\u8fc7\u5176\u4ed6\u8fc7\u6ee4\u540e\u4f20\u5165\u7684\u8ba1\u6570\u4e5f\u53ef\u80fd\u5df2\u7ecf\u51cf\u5c11\u3002<\/p>\n
\u8fd9\u4e00\u70b9\u5728\u8f83\u957f\u6d41\u7a0b\u4e2d\u7279\u522b\u6709\u7528\u3002\u539f\u59cb\u8bfb\u6bb5\u6570\u3001\u901a\u8fc7\u53bb\u566a\u7684\u6570\u91cf\u3001\u53bb\u9664\u7279\u5b9a\u7279\u5f81\u540e\u7684\u603b\u91cf\uff0c\u4ee5\u53ca\u6700\u7ec8\u62bd\u5e73\u524d\u7684\u8ba1\u6570\uff0c\u5c5e\u4e8e\u4e0d\u540c\u9636\u6bb5\u3002\u6392\u67e5\u6837\u672c\u4e22\u5931\u65f6\uff0c\u5e94\u627e\u5230\u7d27\u90bb\u8be5\u6b65\u9aa4\u7684\u8f93\u5165\u6570\u503c\u3002<\/p>\n
\u7279\u5f81\u8fc7\u6ee4\u4e3a\u4ec0\u4e48\u53c8\u8ba9\u4e19\u65e0\u6cd5\u901a\u8fc7<\/h2>\n
\u7b2c\u4e09\u7c7b\u5bf9\u7167\u5148\u8fc7\u6ee4\u7279\u5f81\uff0c\u8981\u6c42\u6bcf\u4e2a\u7279\u5f81\u81f3\u5c11\u51fa\u73b0\u5728\u4e24\u4e2a\u6837\u672c\u4e2d\u3002\u7b2c\u56db\u4e2a\u7279\u5f81\u53ea\u5728\u4e19\u4e2d\u51fa\u73b0\uff0c\u56e0\u6b64\u88ab\u5220\u9664\u3002\u5176\u4ed6\u4e09\u4e2a\u7279\u5f81\u6ee1\u8db3\u6761\u4ef6\uff0c\u5168\u90e8\u4fdd\u7559\u3002<\/p>\n
qiime feature-table filter-features \\\r\n  --i-table counts.qza \\\r\n  --p-min-samples 2 \\\r\n  --o-filtered-table filtered.qza\r\n<\/code><\/pre>\n
\u5b9e\u9645\u5bfc\u51fa\u7684\u8fc7\u6ee4\u8868\u4ecd\u7136\u5305\u542b\u4e09\u4e2a\u6837\u672c\uff0c\u4f46\u603b\u8ba1\u6570\u53d8\u6210\u4e00\u767e\u3001\u5341\u3001\u5341\u3002\u4e19\u4e22\u6389\u7b2c\u56db\u4e2a\u7279\u5f81\u7684\u4e24\u6b21\u8ba1\u6570\uff0c\u4ece\u5341\u4e8c\u964d\u5230\u5341\u3002\u6b64\u65f6\u518d\u6b21\u8bbe\u7f6e\u62bd\u5e73\u6df1\u5ea6\u5341\u4e00\uff0c\u5b9e\u9645\u7ed3\u679c\u53ea\u5269\u7532\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u8f93\u5165\u4e0e\u6761\u4ef6<\/th>\n\u62bd\u5e73\u524d\u603b\u8ba1\u6570<\/th>\n\u62bd\u5e73\u540e\u4fdd\u7559\u6837\u672c<\/th>\n<\/tr>\n<\/thead>\n
\u539f\u59cb\u8868\uff0c\u6df1\u5ea6 10<\/td>\n100\u300110\u300112<\/td>\n\u7532\u3001\u4e59\u3001\u4e19<\/td>\n<\/tr>\n
\u539f\u59cb\u8868\uff0c\u6df1\u5ea6 11<\/td>\n100\u300110\u300112<\/td>\n\u7532\u3001\u4e19<\/td>\n<\/tr>\n
\u5148\u505a\u7279\u5f81\u8fc7\u6ee4\uff0c\u6df1\u5ea6 11<\/td>\n100\u300110\u300110<\/td>\n\u7532<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8fd9\u7ec4\u7ed3\u679c\u8bf4\u660e\uff0c\u6837\u672c\u662f\u5426\u80fd\u901a\u8fc7\u62bd\u5e73\uff0c\u53d6\u51b3\u4e8e\u8be5\u6b65\u9aa4\u5b9e\u9645\u770b\u5230\u7684\u8ba1\u6570\u3002\u7279\u5f81\u8fc7\u6ee4\u867d\u7136\u76f4\u63a5\u4f5c\u7528\u4e8e\u884c\uff0c\u4e5f\u4f1a\u6539\u53d8\u5404\u6837\u672c\u7684\u5217\u603b\u548c\uff0c\u56e0\u6b64\u53ef\u80fd\u5f71\u54cd\u540e\u9762\u7684\u6837\u672c\u4fdd\u7559\u3002<\/p>\n
\u89e3\u91ca\u8fc7\u6ee4\u89c4\u5219\u65f6\uff0c\u8fd8\u5e94\u786e\u8ba4\u201c\u81f3\u5c11\u4e24\u4e2a\u6837\u672c\u201d\u5728\u54ea\u4e00\u7ec4\u6837\u672c\u8303\u56f4\u5185\u8ba1\u7b97\u3002\u5982\u679c\u4e4b\u524d\u5df2\u7ecf\u5220\u6389\u67d0\u4e9b\u6837\u672c\uff0c\u7279\u5f81\u7684\u51fa\u73b0\u6b21\u6570\u4e5f\u4f1a\u53d8\u5316\u3002\u672c\u6b21\u8fc7\u6ee4\u660e\u786e\u5728\u539f\u59cb\u4e09\u4e2a\u6837\u672c\u4e0a\u6267\u884c\uff0c\u4e0b\u8f7d\u5305\u4fdd\u7559\u4e86\u8f93\u5165\u4ea7\u7269\u4e0e\u53c2\u6570\u5173\u7cfb\u3002<\/p>\n
\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u540e\uff0c\u5e94\u8be5\u6bd4\u8f83\u4ec0\u4e48\u6765\u5224\u65ad\u590d\u73b0<\/h2>\n
\u672c\u6b21\u5bf9\u539f\u59cb\u8868\u6309\u6df1\u5ea6\u5341\u4e00\u3001\u79cd\u5b50\u4e00\u767e\u4e8c\u5341\u4e00\u91cd\u590d\u8fd0\u884c\u4e24\u6b21\u3002\u5bfc\u51fa\u540e\uff0c\u4e24\u6b21\u7684\u6837\u672c\u540d\u79f0\u3001\u7279\u5f81\u540d\u79f0\u548c\u8ba1\u6570\u77e9\u9635\u4e00\u81f4\u3002\u6211\u4eec\u53e6\u7528\u79cd\u5b50\u4e00\u767e\u4e8c\u5341\u4e8c\u518d\u8fd0\u884c\u4e00\u6b21\uff0c\u4fdd\u5b58\u4e86\u5bf9\u5e94\u7ed3\u679c\uff0c\u4f9b\u8bfb\u8005\u68c0\u67e5\u62bd\u6837\u6761\u4ef6\u53d8\u5316\u3002<\/p>\n
\u56fa\u5b9a\u79cd\u5b50\u9002\u5408\u8ba9\u540c\u4e00\u8f93\u5165\u3001\u540c\u4e00\u5b9e\u73b0\u4e0b\u7684\u968f\u673a\u6b65\u9aa4\u5f97\u5230\u53ef\u91cd\u590d\u7ed3\u679c\u3002\u4fdd\u7559\u79cd\u5b50\u65f6\uff0c\u8fd8\u5e94\u4e00\u540c\u8bb0\u5f55\u7248\u672c\u4e0e\u8f93\u5165\u4ea7\u7269\uff0c\u56e0\u4e3a\u5b83\u4eec\u5171\u540c\u51b3\u5b9a\u5b9e\u9645\u8ba1\u7b97\u6761\u4ef6\u3002<\/p>\n
\u4e24\u6b21\u76f8\u540c\u79cd\u5b50\u7684\u8fd0\u884c\u4f1a\u751f\u6210\u65b0\u7684\u4ea7\u7269\u6807\u8bc6\u548c\u8fd0\u884c\u8bb0\u5f55\u3002\u56e0\u6b64\uff0c\u6574\u4e2a\u538b\u7f29\u4ea7\u7269\u7684\u5b57\u8282\u5185\u5bb9\u53ef\u4ee5\u4e0d\u540c\uff0c\u5373\u4f7f\u5176\u4e2d\u7684\u8ba1\u6570\u77e9\u9635\u4e00\u81f4\u3002\u5224\u65ad\u672c\u4f8b\u7684\u6570\u503c\u590d\u73b0\u65f6\uff0c\u6211\u4eec\u6bd4\u8f83\u7684\u662f\u89e3\u51fa\u540e\u7684\u540d\u79f0\u4e0e\u77e9\u9635\uff0c\u540c\u65f6\u68c0\u67e5\u6765\u6e90\u4e2d\u8bb0\u5f55\u7684\u8f93\u5165\u548c\u53c2\u6570\u3002<\/p>\n
\u8fd9\u79cd\u6bd4\u8f83\u65b9\u5f0f\u4e5f\u9002\u5408\u534f\u4f5c\u3002\u4ea4\u4ed8\u7ed3\u679c\u65f6\uff0c\u53ef\u4ee5\u628a\u6570\u636e\u5185\u5bb9\u7684\u6838\u5bf9\u4e0e\u8fd0\u884c\u8bb0\u5f55\u7684\u6838\u5bf9\u5206\u5f00\u5b8c\u6210\u3002\u524d\u8005\u786e\u8ba4\u5177\u4f53\u6570\u503c\uff0c\u540e\u8005\u786e\u8ba4\u8fd9\u4e9b\u6570\u503c\u901a\u8fc7\u54ea\u4e9b\u6761\u4ef6\u4ea7\u751f\uff0c\u4e8c\u8005\u90fd\u5bf9\u590d\u67e5\u6709\u7528\u3002<\/p>\n
\u672c\u6b21\u4e24\u4e2a\u79cd\u5b50\u7684\u5b9e\u9645\u5dee\u522b\u53ef\u4ee5\u5728\u7532\u6837\u672c\u4e2d\u76f4\u63a5\u770b\u5230\u3002\u79cd\u5b50\u4e00\u767e\u4e8c\u5341\u4e00\u62bd\u5230\u7684\u524d\u4e09\u4e2a\u7279\u5f81\u8ba1\u6570\u5206\u522b\u4e3a\u4e5d\u3001\u4e8c\u3001\u96f6\uff0c\u79cd\u5b50\u4e00\u767e\u4e8c\u5341\u4e8c\u5219\u4e3a\u516b\u3001\u4e8c\u3001\u4e00\uff0c\u5408\u8ba1\u90fd\u4e3a\u5341\u4e00\u3002\u7b2c\u4e09\u4e2a\u7279\u5f81\u5728\u539f\u59cb\u7532\u6837\u672c\u4e2d\u6709\u4e94\u4e2a\u8ba1\u6570\uff0c\u4f46\u4e00\u6b21\u62bd\u6837\u4e2d\u6ca1\u6709\u88ab\u62bd\u5230\uff0c\u53e6\u4e00\u6b21\u5219\u88ab\u62bd\u5230\u4e00\u4e2a\u3002<\/p>\n
\u56e0\u6b64\uff0c\u62bd\u5e73\u540e\u7684\u96f6\u503c\u8fd8\u9700\u8981\u7ed3\u5408\u4e0a\u6e38\u8868\u683c\u89e3\u91ca\u3002\u5b83\u53ef\u80fd\u8868\u793a\u539f\u59cb\u8868\u4e2d\u5c31\u6ca1\u6709\u89c2\u6d4b\uff0c\u4e5f\u53ef\u80fd\u6765\u81ea\u8fc7\u6ee4\u6216\u672c\u6b21\u968f\u673a\u62bd\u6837\u3002\u8ba8\u8bba\u67d0\u4e2a\u7279\u5f81\u662f\u5426\u5b58\u5728\u65f6\uff0c\u4fdd\u7559\u62bd\u5e73\u524d\u7684\u8ba1\u6570\u4e0e\u5904\u7406\u6761\u4ef6\uff0c\u53ef\u4ee5\u5e2e\u52a9\u533a\u5206\u8fd9\u4e9b\u6765\u6e90\u3002\u672c\u6b21\u8fc7\u6ee4\u540e\u518d\u62bd\u5e73\u7684\u7ed3\u679c\u53ea\u5269\u7532\uff0c\u7b2c\u4e09\u4e2a\u7279\u5f81\u5728\u4fdd\u7559\u6837\u672c\u4e2d\u5168\u4e3a\u96f6\uff0c\u6700\u7ec8\u8868\u4e5f\u53ea\u4fdd\u7559\u4e86\u524d\u4e24\u4e2a\u7279\u5f81\u3002<\/p>\n
\u628a\u76f8\u5bf9\u9891\u7387\u8868\u62ff\u53bb\u62bd\u5e73\uff0c\u4f1a\u53d1\u751f\u4ec0\u4e48<\/h2>\n
\u672c\u6b21\u8fd8\u6b63\u5e38\u751f\u6210\u4e86\u76f8\u5bf9\u9891\u7387\u4ea7\u7269\uff0c\u5404\u6837\u672c\u6309\u5217\u5f52\u4e00\u5316\u3002\u968f\u540e\u5c1d\u8bd5\u628a\u5b83\u4f20\u7ed9\u540c\u4e00\u4e2a\u62bd\u5e73\u547d\u4ee4\uff0c\u7a0b\u5e8f\u56e0\u8f93\u5165\u7c7b\u578b\u4e0d\u5339\u914d\u800c\u62d2\u7edd\u6267\u884c\u3002<\/p>\n
\u8fd9\u9879\u5bf9\u7167\u5c55\u793a\u4e86\u7c7b\u578b\u4fe1\u606f\u7684\u5b9e\u9645\u4f5c\u7528\u3002\u62bd\u5e73\u9700\u8981\u8ba1\u6570\u5f62\u5f0f\u7684\u8f93\u5165\uff0c\u76f8\u5bf9\u9891\u7387\u8868\u8868\u793a\u6bd4\u4f8b\uff0c\u5373\u4f7f\u4e5f\u5b58\u653e\u5728 BIOM \u6587\u4ef6\u4e2d\uff0c\u4ecd\u7136\u4e0d\u80fd\u636e\u6b64\u6062\u590d\u539f\u59cb\u89c2\u6d4b\u6570\u91cf\u3002\u4fdd\u7559\u539f\u59cb\u8ba1\u6570\u8868\uff0c\u53ef\u4ee5\u907f\u514d\u540e\u7eed\u9700\u8981\u8ba1\u6570\u65f6\u5931\u53bb\u6765\u6e90\u3002<\/p>\n
qiime feature-table relative-frequency \\\r\n  --i-table counts.qza \\\r\n  --o-relative-frequency-table relative.qza\r\n<\/code><\/pre>\n
\u9047\u5230\u8fd9\u7c7b\u9519\u8bef\u65f6\uff0c\u5e94\u56de\u5230\u5206\u6790\u542b\u4e49\u9009\u62e9\u6b63\u786e\u8f93\u5165\u3002\u5c06\u6587\u4ef6\u6539\u540d\u3001\u4fee\u6539\u540e\u7f00\u6216\u8005\u628a\u6bd4\u4f8b\u968f\u610f\u4e58\u4ee5\u4e00\u4e2a\u5e38\u6570\uff0c\u90fd\u4e0d\u80fd\u4fdd\u8bc1\u6062\u590d\u539f\u6765\u7684\u89c2\u6d4b\u8ba1\u6570\u3002\u5e94\u8be5\u627e\u5230\u4ecd\u7136\u4fdd\u7559\u771f\u5b9e\u8ba1\u6570\u7684\u4ea7\u7269\uff0c\u518d\u6267\u884c\u9700\u8981\u8ba1\u6570\u7684\u6b65\u9aa4\u3002<\/p>\n
\u672c\u4f8b\u5c06\u9884\u671f\u5931\u8d25\u7684\u7c7b\u578b\u68c0\u67e5\u4fdd\u5b58\u5728\u65e5\u5fd7\u4e2d\uff0c\u968f\u540e\u7ee7\u7eed\u5b8c\u6210\u4ea7\u7269\u9a8c\u8bc1\u3002\u590d\u73b0\u811a\u672c\u4f1a\u660e\u786e\u68c0\u67e5\u8fd9\u4e00\u6b65\u786e\u5b9e\u8fd4\u56de\u5931\u8d25\u72b6\u6001\uff0c\u907f\u514d\u628a\u9884\u671f\u7684\u8f93\u5165\u62d2\u7edd\u8bef\u8bfb\u4e3a\u5b9e\u9a8c\u4e2d\u65ad\u3002<\/p>\n
\u7528\u6765\u6e90\u8bb0\u5f55\u4e32\u8d77\u6574\u4e2a\u5904\u7406\u8fc7\u7a0b<\/h2>\n
\u672c\u6b21\u8bfb\u53d6\u4ea7\u7269\u4e2d\u7684\u6765\u6e90\u4fe1\u606f\uff0c\u6838\u5bf9\u62bd\u5e73\u6b65\u9aa4\u4f7f\u7528\u7684\u8f93\u5165\u4ea7\u7269\u3001\u6df1\u5ea6\u4e0e\u79cd\u5b50\uff0c\u4ee5\u53ca\u8fc7\u6ee4\u6b65\u9aa4\u5f62\u6210\u7684\u4e0a\u6e38\u5173\u7cfb\u3002\u8fd9\u6837\u53ef\u4ee5\u89e3\u91ca\u4e3a\u4ec0\u4e48\u4e24\u5f20\u770b\u4f3c\u76f8\u4f3c\u7684\u62bd\u5e73\u8868\uff0c\u6700\u7ec8\u4fdd\u7559\u7684\u6837\u672c\u4e0d\u540c\u3002<\/p>\n
\u540c\u65f6\uff0c\u6211\u4eec\u901a\u8fc7\u6b63\u5e38\u5bfc\u51fa\u83b7\u53d6\u5185\u90e8\u6570\u636e\uff0c\u518d\u8bfb\u53d6\u6837\u672c\u5217\u8868\u3001\u7279\u5f81\u5217\u8868\u53ca\u77e9\u9635\u3002\u5bfc\u51fa\u6570\u636e\u9002\u5408\u7ee7\u7eed\u4ea4\u7ed9\u5176\u4ed6\u5de5\u5177\u5904\u7406\uff0c\u539f\u59cb\u4ea7\u7269\u9002\u5408\u4fdd\u7559\u6765\u6e90\u5173\u7cfb\uff0c\u4e24\u8005\u53ef\u4ee5\u4e00\u8d77\u4fdd\u5b58\u3002\u5b98\u65b9\u5bfc\u51fa\u8bf4\u660e<\/a><\/p>\n
\u6765\u6e90\u8bb0\u5f55\u4ece\u5bfc\u5165\u4e4b\u540e\u7684\u6d41\u7a0b\u5f00\u59cb\u63d0\u4f9b\u652f\u6301\u3002\u5bf9\u4e8e\u672c\u6b21\u4eba\u5de5\u751f\u6210\u7684\u8ba1\u6570\uff0c\u751f\u6210\u811a\u672c\u4e5f\u9700\u8981\u4e00\u540c\u4fdd\u5b58\u3002\u771f\u5b9e\u9879\u76ee\u82e5\u5728\u5bfc\u5165\u524d\u8fdb\u884c\u4e86\u5176\u4ed6\u5904\u7406\uff0c\u540c\u6837\u5e94\u4fdd\u5b58\u76f8\u5e94\u65b9\u6cd5\u548c\u8f93\u5165\uff0c\u624d\u80fd\u5f62\u6210\u8fde\u7eed\u7684\u5206\u6790\u8bb0\u5f55\u3002<\/p>\n
QIIME 2 \u7684\u4ef7\u503c\u5728\u4e8e\u5c06\u7c7b\u578b\u68c0\u67e5\u3001\u63d2\u4ef6\u64cd\u4f5c\u548c\u6765\u6e90\u8bb0\u5f55\u8fde\u63a5\u8d77\u6765\u3002\u4f7f\u7528\u65f6\u9700\u8981\u6295\u5165\u4e00\u4e9b\u7cbe\u529b\u7406\u89e3\u8fd9\u4e9b\u5bf9\u8c61\uff0c\u4f46\u638c\u63e1\u4e4b\u540e\uff0c\u6837\u672c\u4e22\u5931\u3001\u8f93\u5165\u8bef\u7528\u4ee5\u53ca\u53c2\u6570\u5dee\u5f02\u90fd\u4f1a\u66f4\u5bb9\u6613\u8ffd\u8e2a\u3002<\/p>\n
\u672c\u6b21\u4e0b\u8f7d\u5305\u5305\u542b\u8ba1\u6570\u751f\u6210\u811a\u672c\u3001\u5168\u90e8\u547d\u4ee4\u3001\u4e94\u7ec4\u62bd\u5e73\u7ed3\u679c\u3001\u7c7b\u578b\u68c0\u67e5\u548c\u4ea7\u7269\u6765\u6e90\u8bb0\u5f55\uff0c\u8bfb\u8005\u53ef\u4ee5\u4fee\u6539\u539f\u59cb\u8ba1\u6570\u6216\u95e8\u69db\u540e\u9010\u9879\u590d\u7b97\u3002\u4e0b\u8f7d\u5b9e\u8dd1\u811a\u672c\u4e0e\u672c\u6b21\u7ed3\u679c<\/a><\/p>\n
\u5f53\u5206\u6790\u9700\u8981\u6301\u7eed\u7ba1\u7406\u591a\u4e2a\u6279\u6b21\u7684\u6269\u589e\u5b50\u6570\u636e\u3001\u63d2\u4ef6\u73af\u5883\u548c\u53ef\u8ffd\u6eaf\u7ed3\u679c\u65f6\uff0c\u53ef\u4e86\u89e3\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u8ba1\u7b97\u670d\u52a1<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u5b9e\u8dd1 QIIME 2 \u8ba1\u6570\u5bfc\u5165\u3001\u7279\u5f81\u8fc7\u6ee4\u548c\u4e94\u7ec4\u62bd\u5e73\uff0c\u89e3\u91ca\u6837\u672c\u4e22\u5931\u3001\u76f8\u5bf9\u9891\u7387\u7c7b\u578b\u9519\u8bef\uff0c\u4ee5\u53ca\u5982\u4f55\u6838\u5bf9\u6570\u503c\u548c\u6765\u6e90\u3002<\/p>\n","protected":false},"author":1,"featured_media":72756,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[1278,1279,1280],"class_list":["post-72757","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-qiime2","tag-1279","tag-1280"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/qiime2-feature-filter-rarefaction-sample-loss-provenance-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72757","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=72757"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72757\/revisions"}],"predecessor-version":[{"id":72760,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72757\/revisions\/72760"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/72756"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=72757"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=72757"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=72757"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}