{"id":72770,"date":"2026-11-15T10:44:21","date_gmt":"2026-11-15T02:44:21","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72770"},"modified":"2026-11-15T10:47:17","modified_gmt":"2026-11-15T02:47:17","slug":"dada2-truncation-overlap-filtering-merging-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/dada2-truncation-overlap-filtering-merging-practice\/","title":{"rendered":"DADA2 \u622a\u77ed\u540e\u901a\u8fc7\u8d28\u63a7\u7684\u8bfb\u6bb5\u66f4\u591a\uff0c\u4e3a\u4ec0\u4e48\u5408\u5e76\u7ed3\u679c\u53cd\u800c\u4e3a\u96f6\uff1f\u7528\u4e24\u4e2a\u793a\u4f8b\u6837\u672c\u5b9e\u8dd1\u6392\u67e5"},"content":{"rendered":"
\n

\u6269\u589e\u5b50\u53cc\u7aef\u5206\u6790\u4e2d\uff0c\u622a\u6389\u4f4e\u8d28\u91cf\u5c3e\u90e8\u5f80\u5f80\u80fd\u591f\u63d0\u9ad8\u8fc7\u6ee4\u901a\u8fc7\u7387\u3002\u4f46\u5982\u679c\u53ea\u89c2\u5bdf\u8fc7\u6ee4\u540e\u7684\u8bfb\u6bb5\u6570\u91cf\uff0c\u53ef\u80fd\u9009\u51fa\u4e00\u7ec4\u6700\u7ec8\u65e0\u6cd5\u5408\u5e76\u7684\u53c2\u6570\u3002\u524d\u540e\u4e24\u4e2a\u65b9\u5411\u5206\u522b\u901a\u8fc7\u8d28\u91cf\u68c0\u67e5\uff0c\u5e76\u4e0d\u4fdd\u8bc1\u5b83\u4eec\u4ecd\u7136\u62e5\u6709\u8db3\u591f\u7684\u91cd\u53e0\u533a\u57df\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u8fd0\u884c DADA2 1.38.0\uff0c\u4f7f\u7528\u8f6f\u4ef6\u5305\u81ea\u5e26\u7684\u4e24\u4e2a\u914d\u5bf9 FASTQ \u793a\u4f8b\u3002\u6211\u4eec\u5b8c\u6210\u8fc7\u6ee4\u3001\u9519\u8bef\u7387\u5b66\u4e60\u3001\u53bb\u5197\u4f59\u3001\u53bb\u566a\u3001\u53cc\u7aef\u5408\u5e76\u548c\u53bb\u5d4c\u5408\u4f53\u68c0\u67e5\uff0c\u5e76\u6bd4\u8f83\u4e24\u7ec4\u622a\u65ad\u957f\u5ea6\u4e0e\u4e24\u4e2a\u5408\u5e76\u91cd\u53e0\u95e8\u69db\u3002\u6bcf\u4e00\u6b65\u7684\u4fdd\u7559\u6570\u91cf\u548c\u5019\u9009\u5408\u5e76\u8bb0\u5f55\u90fd\u4fdd\u5b58\u4e0b\u6765\uff0c\u4fbf\u4e8e\u89e3\u91ca\u8bfb\u6bb5\u5728\u54ea\u4e2a\u73af\u8282\u51cf\u5c11\u3002<\/p>\n

\"\u8d28\u63a7\u901a\u8fc7\u66f4\u591a\uff0c\u5408\u5e76\u4e3a\u4f55\u4e3a\u96f6\u3002DADA2
DADA2 \u4e24\u4e2a\u793a\u4f8b\u6837\u672c\u7684\u5b8c\u6574\u6570\u91cf\u6838\u5bf9<\/figcaption><\/figure>\n

\u4e3a\u4ec0\u4e48\u8981\u628a\u8d28\u91cf\u548c\u91cd\u53e0\u957f\u5ea6\u4e00\u8d77\u8003\u8651<\/h2>\n

\u53cc\u7aef\u8bfb\u6bb5\u6765\u81ea\u540c\u4e00\u4e2a\u6269\u589e\u5b50\u4e24\u7aef\u3002\u7ecf\u8fc7\u65b9\u5411\u8f6c\u6362\u540e\uff0c\u5982\u679c\u4e8c\u8005\u8986\u76d6\u8303\u56f4\u91cd\u53e0\uff0c\u5c31\u53ef\u4ee5\u5728\u91cd\u53e0\u533a\u6838\u5bf9\u5e8f\u5217\u5e76\u5c1d\u8bd5\u62fc\u63a5\u3002\u622a\u77ed\u4f1a\u51cf\u5c11\u4f4e\u8d28\u91cf\u4f4d\u7f6e\uff0c\u4e5f\u4f1a\u7f29\u77ed\u53ef\u7528\u4e8e\u91cd\u53e0\u7684\u90e8\u5206\u3002<\/p>\n

\u5bf9\u4e8e\u6ca1\u6709\u989d\u5916\u590d\u6742\u53d8\u5316\u7684\u7247\u6bb5\uff0c\u53ef\u4ee5\u5148\u7528\u524d\u5411\u4fdd\u7559\u957f\u5ea6\u52a0\u53cd\u5411\u4fdd\u7559\u957f\u5ea6\uff0c\u518d\u51cf\u53bb\u76ee\u6807\u7247\u6bb5\u957f\u5ea6\uff0c\u4f30\u7b97\u53ef\u80fd\u7684\u91cd\u53e0\u8303\u56f4\u3002\u8fd9\u4e2a\u4f30\u7b97\u9002\u5408\u5728\u6b63\u5f0f\u5904\u7406\u524d\u6392\u9664\u660e\u663e\u4e0d\u53ef\u884c\u7684\u7ec4\u5408\uff0c\u5b9e\u9645\u63a5\u53d7\u4e0e\u5426\u8fd8\u8981\u770b\u6bd4\u5bf9\u548c\u9519\u914d\u6761\u4ef6\u3002<\/p>\n

DADA2 \u7684\u6807\u51c6\u6d41\u7a0b\u5148\u5206\u522b\u5904\u7406\u4e24\u4e2a\u65b9\u5411\uff0c\u518d\u5408\u5e76\u5bf9\u5e94\u7684\u63a8\u65ad\u5e8f\u5217\u3002\u5b98\u65b9\u6559\u7a0b\u4e5f\u8981\u6c42\u8f93\u5165\u4fdd\u6301\u6b63\u786e\u7684\u6837\u672c\u5bf9\u5e94\u3001\u914d\u5bf9\u987a\u5e8f\u4ee5\u53ca\u9002\u5f53\u7684\u524d\u5904\u7406\u72b6\u6001\u3002\u5b98\u65b9\u6d41\u7a0b\u6559\u7a0b<\/a><\/p>\n

\u672c\u6b21\u91cd\u70b9\u68c0\u67e5\u622a\u65ad\u4e0e\u5408\u5e76\u7684\u5173\u7cfb\u3002\u4e24\u4e2a\u8f93\u5165\u6587\u4ef6\u4e2d\u7684\u6837\u672c\u53ea\u662f\u8f6f\u4ef6\u5305\u793a\u4f8b\uff0c\u6211\u4eec\u6ca1\u6709\u4e3a\u5b83\u4eec\u8865\u5145\u672a\u7ecf\u9a8c\u8bc1\u7684\u5b9e\u9a8c\u5206\u7ec4\u6216\u6765\u6e90\u80cc\u666f\uff0c\u4e5f\u6ca1\u6709\u4f7f\u7528\u8fd9\u4e9b\u5c0f\u6570\u636e\u63a8\u65ad\u771f\u5b9e\u7fa4\u843d\u5dee\u5f02\u3002<\/p>\n

\u672c\u6b21\u73af\u5883\u4e0e\u8f93\u5165\u600e\u6837\u56fa\u5b9a<\/h2>\n

\u5b9e\u9a8c\u73af\u5883\u4e2d R \u7684\u7248\u672c\u4e3a 4.5.2\uff0cDADA2 \u4e3a 1.38.0\u3002\u8f6f\u4ef6\u968f\u672c\u6b21\u5b89\u88c5\u7684\u5b98\u65b9 QIIME 2 \u53d1\u884c\u73af\u5883\u63d0\u4f9b\uff0c\u6211\u4eec\u76f4\u63a5\u8c03\u7528\u5176 R \u5305\u51fd\u6570\u5b8c\u6210\u5206\u6790\uff0c\u5b9e\u9645\u7248\u672c\u901a\u8fc7\u7a0b\u5e8f\u8bfb\u53d6\u5e76\u4fdd\u5b58\u3002<\/p>\n

\u8f93\u5165\u6765\u81ea DADA2 \u5b89\u88c5\u76ee\u5f55\u7684\u793a\u4f8b\u6587\u4ef6\uff0c\u6bcf\u4e2a\u6837\u672c\u5305\u542b\u524d\u5411\u548c\u53cd\u5411\u5404\u4e00\u4e2a\u538b\u7f29 FASTQ\u3002\u6bcf\u4e2a\u6837\u672c\u5b9e\u9645\u8bfb\u5165\u4e00\u5343\u4e94\u767e\u5bf9\u8bfb\u6bb5\u3002\u4e0b\u8f7d\u5305\u8bb0\u5f55\u4e86\u56db\u4e2a\u8f93\u5165\u6587\u4ef6\u7684\u6821\u9a8c\u503c\uff0c\u56e0\u6b64\u590d\u73b0\u65f6\u53ef\u4ee5\u786e\u8ba4\u662f\u5426\u4f7f\u7528\u4e86\u76f8\u540c\u6587\u4ef6\u3002<\/p>\n

library(dada2)\r\npackageVersion("dada2")\r\n\r\nexample <- system.file("extdata", package="dada2")\r\nsamples <- c("sam1", "sam2")\r\nfwd <- file.path(example, paste0(samples, "F.fastq.gz"))\r\nrev <- file.path(example, paste0(samples, "R.fastq.gz"))\r\ndir.create("filtered", showWarnings=FALSE)\r\nfiltF <- file.path("filtered", paste0(samples, "F.fq.gz"))\r\nfiltR <- file.path("filtered", paste0(samples, "R.fq.gz"))\r\n<\/code><\/pre>\n
\u793a\u4f8b\u6587\u4ef6\u9002\u5408\u68c0\u67e5\u64cd\u4f5c\u548c\u7ed3\u679c\u5173\u7cfb\u3002\u6b63\u5f0f\u9879\u76ee\u4e2d\u7684\u9519\u8bef\u7387\u6a21\u578b\u9700\u8981\u7ed3\u5408\u5b9e\u9645\u6d4b\u5e8f\u6279\u6b21\u3001\u8bfb\u6bb5\u8d28\u91cf\u4e0e\u8db3\u591f\u6570\u636e\u8bc4\u4f30\u3002\u672c\u6b21\u6570\u636e\u91cf\u5f88\u5c0f\uff0c\u540e\u6587\u8ba8\u8bba\u7684\u662f\u8fd9\u4e9b\u8f93\u5165\u4e0b\u7684\u5b9e\u9645\u884c\u4e3a\uff0c\u6ca1\u6709\u628a\u5b83\u5f53\u4f5c\u751f\u4ea7\u5206\u6790\u7684\u8d28\u91cf\u57fa\u51c6\u3002DADA2 \u9879\u76ee\u4e0e\u5b89\u88c5\u8d44\u6599<\/a><\/p>\n
\u7b2c\u4e00\u7ec4\u4fdd\u7559\u524d\u5411\u4e8c\u767e\u56db\u5341\u3001\u53cd\u5411\u4e00\u767e\u516d\u5341\u78b1\u57fa<\/h2>\n
\u7b2c\u4e00\u7ec4\u5c06\u524d\u5411\u8bfb\u6bb5\u622a\u5230\u4e8c\u767e\u56db\u5341\u3001\u53cd\u5411\u622a\u5230\u4e00\u767e\u516d\u5341\u3002\u4e24\u4e2a\u65b9\u5411\u7684\u6700\u5927\u671f\u671b\u9519\u8bef\u6570\u90fd\u8bbe\u4e3a\u4e8c\uff0c\u4e0d\u5141\u8bb8\u672a\u77e5\u78b1\u57fa\uff0c\u5176\u4ed6\u4e3b\u8981\u8fc7\u6ee4\u6761\u4ef6\u6309\u4ee3\u7801\u56fa\u5b9a\u3002<\/p>\n
filtered <- filterAndTrim(\r\n  fwd, filtF, rev, filtR,\r\n  truncLen=c(240, 160),\r\n  maxN=0,\r\n  maxEE=c(2, 2),\r\n  truncQ=2,\r\n  rm.phix=TRUE,\r\n  compress=TRUE,\r\n  multithread=FALSE\r\n)\r\n<\/code><\/pre>\n
\u5b9e\u9645\u8fc7\u6ee4\u540e\uff0c\u6837\u672c\u4e00\u4fdd\u7559\u4e00\u5343\u96f6\u4e8c\u5341\u4e00\u5bf9\uff0c\u6837\u672c\u4e8c\u4fdd\u7559\u4e00\u5343\u96f6\u4e5d\u5bf9\u3002\u8fd9\u91cc\u7684\u6570\u91cf\u662f\u914d\u5bf9\u8fc7\u6ee4\u540e\u4fdd\u7559\u7684\u5bf9\u6570\uff0c\u9700\u8981\u6309\u4e24\u4e2a\u65b9\u5411\u914d\u5957\u7406\u89e3\uff0c\u4e0d\u80fd\u628a\u524d\u540e\u5411\u6587\u4ef6\u4e2d\u7684\u8bb0\u5f55\u6570\u518d\u76f8\u52a0\u5f53\u4f5c\u6837\u672c\u7684\u72ec\u7acb\u7247\u6bb5\u6570\u3002<\/p>\n
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\u6392\u67e5\u81ea\u5df1\u7684\u6570\u636e\u65f6\uff0c\u53ef\u4ee5\u4fdd\u7559\u6bcf\u4e2a\u6837\u672c\u7684\u8f93\u5165\u91cf\u3001\u8fc7\u6ee4\u91cf\u53ca\u4fdd\u7559\u6bd4\u4f8b\uff0c\u518d\u7ed3\u5408\u8d28\u91cf\u56fe\u9009\u62e9\u5019\u9009\u622a\u65ad\u7ec4\u5408\u3002\u6b64\u65f6\u5f97\u5230\u7684\u662f\u8fdb\u5165\u540e\u7eed\u5206\u6790\u7684\u5019\u9009\u8f93\u5165\uff0c\u6700\u7ec8\u80fd\u4fdd\u7559\u591a\u5c11\u7247\u6bb5\u8fd8\u9700\u8981\u7ee7\u7eed\u5f80\u4e0b\u68c0\u67e5\u3002<\/p>\n
\u9519\u8bef\u7387\u5b66\u4e60\u548c\u53bb\u566a\u5728\u672c\u4f8b\u4e2d\u600e\u6837\u8fd0\u884c<\/h2>\n
\u6211\u4eec\u7528\u7b2c\u4e00\u7ec4\u8fc7\u6ee4\u540e\u7684\u4e24\u4e2a\u6837\u672c\u5206\u522b\u5b66\u4e60\u524d\u5411\u548c\u53cd\u5411\u9519\u8bef\u7387\uff0c\u518d\u4fdd\u5b58\u4e24\u4e2a\u6a21\u578b\u3002\u968f\u540e\u5bf9\u4e24\u4e2a\u65b9\u5411\u5206\u522b\u53bb\u5197\u4f59\uff0c\u5e76\u5b9e\u9645\u8c03\u7528\u53bb\u566a\u51fd\u6570\u3002<\/p>\n
errF <- learnErrors(\r\n  filtF, nbases=1000000,\r\n  multithread=2, randomize=FALSE\r\n)\r\nerrR <- learnErrors(\r\n  filtR, nbases=1000000,\r\n  multithread=2, randomize=FALSE\r\n)\r\n\r\nderepF <- derepFastq(filtF)\r\nderepR <- derepFastq(filtR)\r\ndadaF <- dada(derepF, err=errF, multithread=2)\r\ndadaR <- dada(derepR, err=errR, multithread=2)\r\n<\/code><\/pre>\n
\u5b9e\u9645\u53bb\u566a\u540e\uff0c\u6837\u672c\u4e00\u524d\u5411\u4fdd\u7559\u4e00\u5343\u96f6\u4e00\u5341\u4e03\u4e2a\u8bfb\u6bb5\uff0c\u53cd\u5411\u4fdd\u7559\u4e00\u5343\u96f6\u4e8c\u5341\u4e00\u4e2a\u3002\u6837\u672c\u4e8c\u524d\u5411\u4fdd\u7559\u4e00\u5343\u96f6\u4e94\u4e2a\uff0c\u53cd\u5411\u4fdd\u7559\u4e00\u5343\u96f6\u516d\u4e2a\u3002\u8fd9\u4e9b\u662f\u4e24\u4e2a\u65b9\u5411\u5404\u81ea\u7684\u7ed3\u679c\uff0c\u4e0b\u4e00\u6b65\u8fd8\u8981\u4f9d\u636e\u539f\u59cb\u914d\u5bf9\u5173\u7cfb\u8fde\u63a5\u3002<\/p>\n
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\u5c06\u6a21\u578b\u6587\u4ef6\u548c\u53bb\u566a\u7ed3\u679c\u4e00\u8d77\u4fdd\u5b58\uff0c\u8fd8\u80fd\u5e2e\u52a9\u590d\u67e5\u4e00\u4e2a\u53c2\u6570\u5bf9\u7167\u662f\u5426\u6df7\u5165\u4e86\u5176\u4ed6\u53d8\u5316\u3002\u82e5\u6bcf\u6b21\u540c\u65f6\u6539\u53d8\u8fc7\u6ee4\u3001\u5b66\u4e60\u6570\u636e\u548c\u5408\u5e76\u95e8\u69db\uff0c\u5c31\u96be\u4ee5\u89e3\u91ca\u6700\u7ec8\u5dee\u5f02\u4e3b\u8981\u6765\u81ea\u54ea\u91cc\u3002<\/p>\n
\u6b63\u5e38\u91cd\u53e0\u95e8\u69db\u4e0b\uff0c\u5b9e\u9645\u5408\u5e76\u4e86\u591a\u5c11\u8bfb\u6bb5<\/h2>\n
\u7b2c\u4e00\u7ec4\u91c7\u7528\u5341\u4e8c\u4e2a\u78b1\u57fa\u7684\u6700\u5c0f\u91cd\u53e0\uff0c\u5141\u8bb8\u7684\u91cd\u53e0\u533a\u9519\u914d\u6570\u4e3a\u96f6\uff0c\u5e76\u4fdd\u7559\u88ab\u62d2\u7edd\u7684\u5019\u9009\u8bb0\u5f55\u3002<\/p>\n
mergers <- mergePairs(\r\n  dadaF, derepF, dadaR, derepR,\r\n  minOverlap=12,\r\n  maxMismatch=0,\r\n  returnRejects=TRUE,\r\n  verbose=TRUE\r\n)\r\n<\/code><\/pre>\n
\u5b9e\u9645\u5408\u5e76\u540e\uff0c\u6837\u672c\u4e00\u4fdd\u7559\u4e5d\u767e\u516d\u5341\u4e94\u5bf9\uff0c\u6837\u672c\u4e8c\u4fdd\u7559\u4e5d\u767e\u4e00\u5341\u4e00\u5bf9\u3002\u5408\u5e76\u5e8f\u5217\u8868\u5171\u5f97\u5230\u516b\u4e2a\u4e0d\u540c\u5e8f\u5217\uff0c\u540e\u7eed\u53bb\u5d4c\u5408\u4f53\u6b65\u9aa4\u6ca1\u6709\u4ece\u8fd9\u516b\u4e2a\u5e8f\u5217\u4e2d\u68c0\u51fa\u9700\u8981\u79fb\u9664\u7684\u5bf9\u8c61\uff0c\u56e0\u6b64\u4e24\u4efd\u6837\u672c\u7684\u6700\u7ec8\u8ba1\u6570\u4fdd\u6301\u4e0d\u53d8\u3002<\/p>\n
\u5927\u90e8\u5206\u63a5\u53d7\u5e8f\u5217\u7684\u957f\u5ea6\u4e3a\u4e8c\u767e\u4e94\u5341\u4e09\uff0c\u53e6\u6709\u4e00\u4e2a\u4e8c\u767e\u4e94\u5341\u4e8c\u78b1\u57fa\u7684\u5e8f\u5217\u3002\u5bf9\u4e8e\u4e8c\u767e\u4e94\u5341\u4e09\u78b1\u57fa\u7684\u7247\u6bb5\uff0c\u524d\u5411\u4e8c\u767e\u56db\u5341\u52a0\u53cd\u5411\u4e00\u767e\u516d\u5341\uff0c\u518d\u51cf\u53bb\u4e8c\u767e\u4e94\u5341\u4e09\uff0c\u5f97\u5230\u4e00\u767e\u56db\u5341\u4e03\u78b1\u57fa\u7684\u91cd\u53e0\u4f30\u7b97\u3002<\/p>\n
\u5019\u9009\u5408\u5e76\u8868\u91cc\uff0c\u591a\u4e2a\u5b9e\u9645\u63a5\u53d7\u8bb0\u5f55\u7684\u5339\u914d\u6570\u6b63\u597d\u4e3a\u4e00\u767e\u56db\u5341\u4e03\uff0c\u9519\u914d\u548c\u63d2\u5165\u7f3a\u5931\u6570\u5747\u4e3a\u96f6\u3002\u8fd9\u8ba9\u622a\u65ad\u957f\u5ea6\u3001\u6700\u7ec8\u5e8f\u5217\u957f\u5ea6\u4e0e\u5b9e\u9645\u5408\u5e76\u8bc1\u636e\u76f8\u4e92\u5bf9\u5e94\u8d77\u6765\u3002<\/p>\n
\u53ea\u63d0\u9ad8\u91cd\u53e0\u95e8\u69db\uff0c\u4e5f\u80fd\u8ba9\u5168\u90e8\u5408\u5e76\u5931\u8d25<\/h2>\n
\u63a5\u4e0b\u6765\u4fdd\u6301\u7b2c\u4e00\u7ec4\u8fc7\u6ee4\u548c\u53bb\u566a\u7ed3\u679c\u4e0d\u53d8\uff0c\u53ea\u5c06\u6700\u5c0f\u91cd\u53e0\u8981\u6c42\u63d0\u9ad8\u5230\u4e00\u767e\u4e94\u5341\u3002\u5b9e\u9645\u5408\u5e76\u6570\u5728\u4e24\u4e2a\u6837\u672c\u4e2d\u90fd\u53d8\u6210\u96f6\u3002<\/p>\n
\u524d\u9762\u5177\u6709\u4e00\u767e\u56db\u5341\u4e03\u4e2a\u5339\u914d\u4f4d\u7f6e\u7684\u4e3b\u8981\u5019\u9009\u4ecd\u7136\u5b58\u5728\uff0c\u5e8f\u5217\u5185\u5bb9\u4e5f\u6ca1\u6709\u53d8\u5316\uff0c\u53ea\u662f\u65e0\u6cd5\u8fbe\u5230\u65b0\u7684\u91cd\u53e0\u6761\u4ef6\u3002\u4e8c\u767e\u4e94\u5341\u4e8c\u78b1\u57fa\u7247\u6bb5\u5bf9\u5e94\u7684\u957f\u5ea6\u5173\u7cfb\u540c\u6837\u4f4e\u4e8e\u8fd9\u4e00\u95e8\u69db\u3002<\/p>\n
\u8fd9\u7ec4\u5bf9\u7167\u8bf4\u660e\uff0c\u5373\u4f7f\u8bfb\u6bb5\u8d28\u91cf\u4e0e\u53bb\u566a\u7ed3\u679c\u90fd\u76f8\u540c\uff0c\u5408\u5e76\u9636\u6bb5\u7684\u8981\u6c42\u672c\u8eab\u4e5f\u8db3\u4ee5\u6539\u53d8\u6700\u7ec8\u4fdd\u7559\u91cf\u3002\u56e0\u6b64\uff0c\u53d1\u73b0\u5408\u5e76\u6570\u5927\u5e45\u4e0b\u964d\u65f6\uff0c\u5e94\u67e5\u770b\u5b9e\u9645\u4f7f\u7528\u7684\u5408\u5e76\u53c2\u6570\u548c\u5019\u9009\u8bb0\u5f55\uff0c\u518d\u51b3\u5b9a\u662f\u5426\u9700\u8981\u8fd4\u56de\u524d\u9762\u7684\u6b65\u9aa4\u3002<\/p>\n
\u8bbe\u7f6e\u66f4\u4e25\u683c\u7684\u95e8\u69db\u53ef\u4ee5\u8868\u8fbe\u4e0d\u540c\u7684\u63a5\u53d7\u6761\u4ef6\uff0c\u4f46\u9700\u8981\u4e0e\u76ee\u6807\u7247\u6bb5\u957f\u5ea6\u76f8\u5bb9\u3002\u5982\u679c\u8f93\u5165\u8bbe\u8ba1\u672c\u6765\u5c31\u65e0\u6cd5\u63d0\u4f9b\u6240\u8981\u6c42\u7684\u91cd\u53e0\uff0c\u63d0\u9ad8\u95e8\u69db\u53ea\u4f1a\u4f7f\u8fd9\u4e9b\u5019\u9009\u88ab\u62d2\u7edd\uff0c\u65e0\u6cd5\u51ed\u7a7a\u589e\u52a0\u53ef\u7528\u8bc1\u636e\u3002<\/p>\n
\u622a\u5230\u4e00\u767e\u4e8c\u5341\u4ee5\u540e\uff0c\u8fc7\u6ee4\u66f4\u591a\u3001\u5408\u5e76\u5374\u4e3a\u96f6<\/h2>\n
\u7b2c\u4e8c\u7ec4\u5c06\u4e24\u4e2a\u65b9\u5411\u90fd\u622a\u5230\u4e00\u767e\u4e8c\u5341\u78b1\u57fa\uff0c\u6700\u5927\u671f\u671b\u9519\u8bef\u7b49\u8fc7\u6ee4\u6761\u4ef6\u4e0e\u524d\u9762\u4fdd\u6301\u4e00\u81f4\u3002\u5b9e\u9645\u8fc7\u6ee4\u901a\u8fc7\u91cf\u660e\u663e\u4e0a\u5347\uff0c\u6837\u672c\u4e00\u4fdd\u7559\u4e00\u5343\u56db\u767e\u4e00\u5341\u516b\u5bf9\uff0c\u6837\u672c\u4e8c\u4fdd\u7559\u4e00\u5343\u56db\u767e\u4e00\u5341\u516d\u5bf9\u3002<\/p>\n
\u53bb\u566a\u540e\u7684\u6570\u91cf\u4e5f\u8f83\u9ad8\u3002\u6837\u672c\u4e00\u524d\u540e\u5411\u5206\u522b\u4fdd\u7559\u4e00\u5343\u56db\u767e\u4e00\u5341\u516d\u548c\u4e00\u5343\u56db\u767e\u4e00\u5341\u4e03\uff0c\u6837\u672c\u4e8c\u5206\u522b\u4fdd\u7559\u4e00\u5343\u56db\u767e\u96f6\u4e5d\u548c\u4e00\u5343\u56db\u767e\u4e00\u5341\u516d\u3002\u4ec5\u67e5\u770b\u8fd9\u4e9b\u4e2d\u95f4\u6570\u91cf\uff0c\u77ed\u622a\u65ad\u7ec4\u4f3c\u4e4e\u66f4\u6709\u5229\u3002<\/p>\n
\u4f46\u4e24\u4e2a\u65b9\u5411\u603b\u5171\u53ea\u6709\u4e8c\u767e\u56db\u5341\u78b1\u57fa\uff0c\u5bf9\u4e3b\u8981\u7684\u4e8c\u767e\u4e94\u5341\u4e09\u78b1\u57fa\u7247\u6bb5\u5df2\u7ecf\u65e0\u6cd5\u5f62\u6210\u5b8c\u6574\u91cd\u53e0\u3002\u5b9e\u9645\u4f7f\u7528\u5341\u4e8c\u78b1\u57fa\u6700\u5c0f\u91cd\u53e0\u8981\u6c42\u65f6\uff0c\u4e24\u4efd\u6837\u672c\u7684\u5408\u5e76\u6570\u90fd\u4e3a\u96f6\uff0c\u5019\u9009\u8bb0\u5f55\u4e2d\u7684\u4e3b\u8981\u914d\u5bf9\u4e5f\u6ca1\u6709\u5339\u914d\u91cd\u53e0\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n\n\n
\u6761\u4ef6<\/th>\n\u6837\u672c<\/th>\n\u8fc7\u6ee4\u540e\u5bf9\u6570<\/th>\n\u5408\u5e76\u540e\u5bf9\u6570<\/th>\n<\/tr>\n<\/thead>\n
240\/160\uff0c\u6700\u5c0f\u91cd\u53e0 12<\/td>\nsam1<\/td>\n1021<\/td>\n965<\/td>\n<\/tr>\n
240\/160\uff0c\u6700\u5c0f\u91cd\u53e0 12<\/td>\nsam2<\/td>\n1009<\/td>\n911<\/td>\n<\/tr>\n
240\/160\uff0c\u6700\u5c0f\u91cd\u53e0 150<\/td>\nsam1<\/td>\n1021<\/td>\n0<\/td>\n<\/tr>\n
240\/160\uff0c\u6700\u5c0f\u91cd\u53e0 150<\/td>\nsam2<\/td>\n1009<\/td>\n0<\/td>\n<\/tr>\n
120\/120\uff0c\u6700\u5c0f\u91cd\u53e0 12<\/td>\nsam1<\/td>\n1418<\/td>\n0<\/td>\n<\/tr>\n
120\/120\uff0c\u6700\u5c0f\u91cd\u53e0 12<\/td>\nsam2<\/td>\n1416<\/td>\n0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u77ed\u622a\u65ad\u7ec4\u4e24\u6837\u672c\u5408\u8ba1\u901a\u8fc7\u8fc7\u6ee4\u4e24\u5343\u516b\u767e\u4e09\u5341\u56db\u5bf9\uff0c\u957f\u622a\u65ad\u7ec4\u5408\u8ba1\u4e24\u5343\u96f6\u4e09\u5341\u5bf9\uff0c\u4f46\u6700\u7ec8\u80fd\u591f\u4f7f\u7528\u7684\u5408\u5e76\u7247\u6bb5\u6765\u81ea\u957f\u622a\u65ad\u7ec4\u3002\u8fd9\u6b63\u662f\u9700\u8981\u8ffd\u8e2a\u5168\u6d41\u7a0b\u4fdd\u7559\u6570\u91cf\u7684\u539f\u56e0\u3002<\/p>\n
\u88ab\u62d2\u7edd\u7684\u5019\u9009\u8868\u8fd8\u53ef\u4ee5\u544a\u8bc9\u6211\u4eec\u4ec0\u4e48<\/h2>\n
\u672c\u6b21\u4fdd\u7559\u4e86\u6240\u6709\u5019\u9009\u5408\u5e76\u8bb0\u5f55\uff0c\u8bfb\u8005\u53ef\u4ee5\u540c\u65f6\u68c0\u67e5\u4e30\u5ea6\u3001\u524d\u540e\u5411\u63a8\u65ad\u5e8f\u5217\u7f16\u53f7\u3001\u5339\u914d\u6570\u3001\u9519\u914d\u6570\u3001\u63d2\u5165\u7f3a\u5931\u6570\u53ca\u662f\u5426\u63a5\u53d7\u3002<\/p>\n
\u4f8b\u5982\uff0c\u6837\u672c\u4e8c\u5728\u6b63\u5e38\u91cd\u53e0\u95e8\u69db\u4e0b\uff0c\u6709\u4e00\u4e2a\u4e30\u5ea6\u4e3a\u4e94\u5341\u4e03\u7684\u5019\u9009\uff0c\u8bb0\u5f55\u4e86\u4e00\u767e\u56db\u5341\u516d\u4e2a\u5339\u914d\u548c\u4e00\u4e2a\u9519\u914d\u3002\u5728\u672c\u6b21\u96f6\u9519\u914d\u8981\u6c42\u4e0b\uff0c\u5b83\u88ab\u62d2\u7edd\u3002\u8fd9\u7c7b\u8bb0\u5f55\u80fd\u591f\u5e2e\u52a9\u533a\u5206\u201c\u91cd\u53e0\u4e0d\u8db3\u201d\u4e0e\u201c\u91cd\u53e0\u533a\u4e0d\u4e00\u81f4\u201d\u3002<\/p>\n
\u5982\u679c\u53ea\u4fdd\u5b58\u6700\u7ec8\u63a5\u53d7\u5e8f\u5217\uff0c\u5c31\u770b\u4e0d\u5230\u8fd9\u4e9b\u5931\u8d25\u539f\u56e0\u3002\u9002\u5f53\u4fdd\u5b58\u5019\u9009\u8868\uff0c\u53ef\u4ee5\u8ba9\u5206\u6790\u8005\u5224\u65ad\u8bfb\u6bb5\u635f\u5931\u4e3b\u8981\u96c6\u4e2d\u5728\u54ea\u4e00\u79cd\u60c5\u51b5\uff0c\u518d\u51b3\u5b9a\u662f\u5426\u9700\u8981\u68c0\u67e5\u914d\u5bf9\u3001\u524d\u5904\u7406\u6216\u53c2\u6570\u8bbe\u7f6e\u3002<\/p>\n
\u672c\u6b21\u6ca1\u6709\u4e3a\u4e86\u589e\u52a0\u5408\u5e76\u6570\u91cf\u800c\u4fee\u6539\u9519\u914d\u5141\u8bb8\u503c\u3002\u771f\u5b9e\u9879\u76ee\u662f\u5426\u8c03\u6574\u8fd9\u4e00\u6761\u4ef6\uff0c\u9700\u8981\u7ed3\u5408\u8d28\u91cf\u3001\u9519\u8bef\u6a21\u578b\u4ee5\u53ca\u7814\u7a76\u8bbe\u8ba1\u5224\u65ad\uff0c\u5e76\u8bb0\u5f55\u6539\u53d8\u540e\u5177\u4f53\u63a5\u7eb3\u4e86\u54ea\u4e9b\u5019\u9009\u3002<\/p>\n
\u8fd8\u6709\u4e00\u4e2a\u5bb9\u6613\u5ffd\u7565\u7684\u64cd\u4f5c\u7ec6\u8282\u3002\u4fdd\u7559\u62d2\u7edd\u5019\u9009\u662f\u4e3a\u4e86\u8bca\u65ad\uff0c\u4f46\u6784\u5efa\u6700\u7ec8\u5e8f\u5217\u8868\u65f6\u4ecd\u987b\u53ea\u9009\u7528\u88ab\u63a5\u53d7\u7684\u5408\u5e76\u7ed3\u679c\u3002\u672c\u6b21\u9a71\u52a8\u5148\u6309\u63a5\u53d7\u5b57\u6bb5\u7b5b\u9009\uff0c\u518d\u8c03\u7528\u5efa\u8868\u548c\u53bb\u5d4c\u5408\u4f53\u51fd\u6570\u3002\u82e5\u628a\u8c03\u8bd5\u7528\u7684\u5168\u90e8\u5019\u9009\u76f4\u63a5\u5f53\u4f5c\u6b63\u5f0f\u7ed3\u679c\uff0c\u53ef\u80fd\u628a\u5931\u8d25\u8bb0\u5f55\u5e26\u5165\u540e\u7eed\u7edf\u8ba1\uff0c\u7834\u574f\u524d\u9762\u5efa\u7acb\u7684\u6570\u91cf\u5173\u7cfb\u3002<\/p>\n
\u672c\u6b21\u53bb\u5d4c\u5408\u4f53\u91c7\u7528\u8de8\u6837\u672c\u5171\u8bc6\u65b9\u5f0f\uff0c\u7ed3\u679c\u6ca1\u6709\u79fb\u9664\u5e8f\u5217\u3002\u8fd9\u53ea\u80fd\u63cf\u8ff0\u5f53\u524d\u4e24\u4e2a\u5c0f\u793a\u4f8b\u53ca\u5f53\u524d\u53c2\u6570\u4e0b\u7684\u8f93\u51fa\uff0c\u4e0d\u80fd\u636e\u6b64\u8ba4\u5b9a\u771f\u5b9e\u9879\u76ee\u4e0d\u5b58\u5728\u5d4c\u5408\u4f53\u3002\u6b63\u5f0f\u6570\u636e\u5e94\u540c\u65f6\u67e5\u770b\u88ab\u79fb\u9664\u7684\u72ec\u7279\u5e8f\u5217\u6570\u4e0e\u5bf9\u5e94\u8bfb\u6bb5\u4e30\u5ea6\uff0c\u907f\u514d\u53ea\u62a5\u544a\u4e00\u4e2a\u603b\u767e\u5206\u6bd4\u3002<\/p>\n
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\u6362\u6210\u81ea\u5df1\u7684\u6570\u636e\uff0c\u5148\u7b97\u54ea\u51e0\u7b14\u8d26<\/h2>\n
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