{"id":72772,"date":"2026-11-15T10:44:23","date_gmt":"2026-11-15T02:44:23","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72772"},"modified":"2026-11-15T10:47:20","modified_gmt":"2026-11-15T02:47:20","slug":"mothur-otu-linkage-distance-threshold-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/mothur-otu-linkage-distance-threshold-practice\/","title":{"rendered":"mothur \u540c\u6837\u6309 0.03 \u8ddd\u79bb\u805a\u7c7b\uff0c\u4e3a\u4ec0\u4e48 OTU \u6570\u91cf\u4e0d\u540c\uff1f\u56db\u6761\u5e8f\u5217\u8dd1\u61c2\u4e09\u79cd\u65b9\u6cd5"},"content":{"rendered":"
\n

\u62ff\u5230\u4e24\u4efd\u6269\u589e\u5b50\u5206\u6790\u7ed3\u679c\u65f6\uff0c\u5982\u679c\u90fd\u5199\u7740\u201c\u6309\u767e\u5206\u4e4b\u4e09\u8ddd\u79bb\u5212\u5206 OTU\u201d\uff0c\u5f88\u591a\u4eba\u4f1a\u4ee5\u4e3a\u5b83\u4eec\u4f7f\u7528\u4e86\u76f8\u540c\u89c4\u5219\u3002\u4f46\u8ddd\u79bb\u9608\u503c\u53ea\u8bf4\u660e\u6bd4\u8f83\u5c3a\u5ea6\uff0c\u5e8f\u5217\u5982\u4f55\u7ec4\u6210\u4e00\u4e2a\u7c07\uff0c\u8fd8\u53d6\u51b3\u4e8e\u805a\u7c7b\u65b9\u6cd5\u3002\u67d0\u4e9b\u65b9\u6cd5\u5141\u8bb8\u901a\u8fc7\u4e2d\u95f4\u6210\u5458\u628a\u8ddd\u79bb\u8f83\u8fdc\u7684\u5e8f\u5217\u8fde\u63a5\u8d77\u6765\uff0c\u53e6\u5916\u4e00\u4e9b\u65b9\u6cd5\u5219\u8981\u6c42\u6240\u6709\u6210\u5458\u4e4b\u95f4\u90fd\u8db3\u591f\u63a5\u8fd1\u3002<\/p>\n

\u672c\u6587\u5b89\u88c5\u5e76\u5b9e\u9645\u8fd0\u884c mothur 1.48.6\uff0c\u7528\u56db\u6761\u5173\u7cfb\u5df2\u77e5\u7684\u5e8f\u5217\u8ba1\u7b97\u8ddd\u79bb\uff0c\u518d\u6bd4\u8f83\u6700\u8fd1\u90bb\u3001\u6700\u8fdc\u90bb\u548c\u5e73\u5747\u90bb\u5c45\u4e09\u79cd\u65b9\u6cd5\u3002\u6211\u4eec\u8fd8\u6269\u5927\u8ba1\u7b97\u8303\u56f4\u91cd\u65b0\u8fd0\u884c\uff0c\u8bfb\u53d6\u6307\u5b9a\u8ddd\u79bb\u4e0b\u7684\u4e30\u5ea6\u8868\uff0c\u68c0\u67e5\u8f93\u51fa\u6807\u7b7e\u4e0e\u5b9e\u9645\u5206\u7ec4\u662f\u5426\u4e00\u81f4\u3002\u8bfb\u5b8c\u53ef\u4ee5\u81ea\u5df1\u590d\u6838\u4e00\u4efd\u805a\u7c7b\u7ed3\u679c\uff0c\u800c\u4e0d\u53ea\u662f\u8bb0\u4f4f\u51e0\u4e2a\u53c2\u6570\u540d\u79f0\u3002<\/p>\n

\"\u540c\u6837\u7684\u9608\u503c\uff0c\u5206\u7ec4\u4e3a\u4f55\u4e0d\u540c\u3002\u56db\u6761\u5e8f\u5217\u5b9e\u8dd1\u4e09\u79cd\u805a\u7c7b\u65b9\u6cd5\u3002\u6700\u8fd1\u90bb\uff0c\u4e24\u4e2a\u5206\u7ec4\uff0cA\u3001B\u3001C
\u56db\u6761\u5e8f\u5217\u5b9e\u8dd1\u4e09\u79cd\u805a\u7c7b\u65b9\u6cd5<\/figcaption><\/figure>\n

\u5148\u628a\u5e8f\u5217\u3001\u8ddd\u79bb\u548c\u805a\u7c7b\u7ed3\u679c\u5206\u5f00<\/h2>\n

\u5e8f\u5217\u6587\u4ef6\u4fdd\u5b58\u78b1\u57fa\uff0c\u8ddd\u79bb\u77e9\u9635\u4fdd\u5b58\u5e8f\u5217\u4e4b\u95f4\u7684\u5dee\u5f02\uff0c\u805a\u7c7b\u7ed3\u679c\u4fdd\u5b58\u54ea\u4e9b\u5e8f\u5217\u88ab\u5f52\u5165\u540c\u4e00\u4e2a\u5355\u4f4d\u3002\u8fd9\u4e09\u5c42\u5bf9\u8c61\u76f8\u4e92\u5173\u8054\uff0c\u4f46\u4e0d\u80fd\u4e92\u76f8\u66ff\u4ee3\u3002\u53ea\u77e5\u9053\u4e24\u4e2a\u5e8f\u5217\u88ab\u5206\u5230\u4e00\u8d77\uff0c\u65e0\u6cd5\u76f4\u63a5\u63a8\u65ad\u5b83\u4eec\u5177\u6709\u5b8c\u5168\u76f8\u540c\u7684\u5e8f\u5217\uff0c\u4e5f\u65e0\u6cd5\u76f4\u63a5\u63a8\u65ad\u5b83\u4eec\u5c5e\u4e8e\u540c\u4e00\u4e2a\u7269\u79cd\u3002<\/p>\n

\u6211\u4eec\u6784\u9020\u56db\u6761\u957f\u5ea6\u5747\u4e3a\u4e00\u5343\u78b1\u57fa\u3001\u6ca1\u6709\u7f3a\u53e3\u7684\u5df2\u5bf9\u9f50\u5e8f\u5217\u3002\u57fa\u7840\u5e8f\u5217\u547d\u540d\u4e3a A\uff0c\u5728\u5176\u4e2d\u4e8c\u5341\u4e2a\u4f4d\u7f6e\u4fee\u6539\u78b1\u57fa\u5f97\u5230 B\uff0c\u518d\u5728\u53e6\u5916\u4e8c\u5341\u4e94\u4e2a\u4f4d\u7f6e\u4fee\u6539 B \u5f97\u5230 C\u3002\u6700\u540e\u4ece A \u51fa\u53d1\uff0c\u5728\u4e0d\u91cd\u53e0\u7684\u516b\u5341\u4e2a\u4f4d\u7f6e\u4fee\u6539\u78b1\u57fa\u5f97\u5230 D\u3002<\/p>\n

\u8fd9\u6837\u7684\u8bbe\u8ba1\u8ba9\u8ddd\u79bb\u5173\u7cfb\u80fd\u591f\u72ec\u7acb\u590d\u7b97\u3002A \u4e0e B \u76f8\u5dee\u4e8c\u5341\u4e2a\u4f4d\u7f6e\uff0cB \u4e0e C \u76f8\u5dee\u4e8c\u5341\u4e94\u4e2a\u4f4d\u7f6e\uff0c\u800c A \u4e0e C \u7684\u5dee\u5f02\u7d2f\u79ef\u4e3a\u56db\u5341\u4e94\u4e2a\u4f4d\u7f6e\u3002\u524d\u4e09\u6761\u5e8f\u5217\u5f62\u6210\u4e00\u6761\u7531\u8fd1\u5230\u8fdc\u7684\u8fde\u63a5\u94fe\uff0cD \u5219\u4f5c\u4e3a\u8f83\u8fdc\u7684\u5bf9\u7167\u3002<\/p>\n

\u8fd9\u4e9b\u662f\u4e3a\u4e86\u68c0\u9a8c\u7b97\u6cd5\u800c\u8bbe\u8ba1\u7684\u5e8f\u5217\uff0c\u6ca1\u6709\u771f\u5b9e\u5206\u7c7b\u540d\u79f0\u3001\u6837\u672c\u5206\u7ec4\u6216\u751f\u6001\u5b66\u80cc\u666f\u3002\u5b83\u4eec\u9002\u5408\u89e3\u91ca\u53c2\u6570\u600e\u6837\u6539\u53d8\u5206\u7ec4\uff0c\u4e0d\u9002\u5408\u636e\u6b64\u8bc4\u4ef7\u67d0\u79cd\u65b9\u6cd5\u5728\u771f\u5b9e\u5fae\u751f\u7269\u7fa4\u843d\u4e2d\u7684\u603b\u4f53\u51c6\u786e\u7387\u3002<\/p>\n

\u5b89\u88c5\u540e\u5148\u6838\u5bf9\u5b9e\u9645\u8fd0\u884c\u7684\u7248\u672c<\/h2>\n

\u672c\u6b21\u4e0b\u8f7d\u5b98\u65b9\u53d1\u5e03\u9875\u63d0\u4f9b\u7684 Ubuntu 22 x86_64 \u65e0 Readline \u4f9d\u8d56\u7248\u672c\uff0c\u89e3\u538b\u540e\u5728\u72ec\u7acb\u76ee\u5f55\u8fd0\u884c\u3002\u7248\u672c\u547d\u4ee4\u5b9e\u9645\u8fd4\u56de 1.48.6\uff0c\u4e0b\u8f7d\u5305\u8fd8\u8bb0\u5f55\u4e86\u53ef\u6267\u884c\u6587\u4ef6\u7684\u6821\u9a8c\u503c\uff0c\u4fbf\u4e8e\u533a\u5206\u540c\u540d\u7a0b\u5e8f\u6216\u4e0d\u540c\u6784\u5efa\u3002mothur 1.48.6 \u5b98\u65b9\u53d1\u5e03\u9875<\/a><\/p>\n

unzip Mothur.Ubuntu_22.x86_64.noReadline.zip -d mothur1486\r\nchmod +x mothur1486\/mothur\/mothur\r\nmothur1486\/mothur\/mothur --version\r\n<\/code><\/pre>\n
\u590d\u73b0\u5305\u4e2d\u7684 Python \u9a71\u52a8\u8d1f\u8d23\u751f\u6210\u5e8f\u5217\u3001\u8c03\u7528\u7a0b\u5e8f\u548c\u8bfb\u53d6\u8f93\u51fa\u3002\u5b83\u4e0d\u66ff\u4ee3 mothur \u7684\u8ddd\u79bb\u8ba1\u7b97\u6216\u805a\u7c7b\u7b97\u6cd5\u3002\u8f93\u5165\u6784\u9020\u4e2d\u7684\u968f\u673a\u79cd\u5b50\u56fa\u5b9a\uff0c\u5177\u4f53\u4fee\u6539\u4f4d\u7f6e\u4e5f\u56fa\u5b9a\uff0c\u56e0\u6b64\u53ef\u4ee5\u4ece\u5934\u91cd\u65b0\u751f\u6210\u76f8\u540c\u8f93\u5165\u3002<\/p>\n
\u5b89\u88c5\u73af\u8282\u5bb9\u6613\u51fa\u73b0\u7684\u6df7\u6dc6\u662f\u7ec8\u7aef\u4e2d\u8c03\u7528\u4e86\u53e6\u4e00\u4e2a\u76ee\u5f55\u91cc\u7684\u7a0b\u5e8f\u3002\u590d\u73b0\u65f6\u53ef\u4ee5\u76f4\u63a5\u4f20\u5165\u53ef\u6267\u884c\u6587\u4ef6\u7684\u5b8c\u6574\u8def\u5f84\uff0c\u518d\u628a\u7248\u672c\u8f93\u51fa\u4e0e\u6587\u4ef6\u6821\u9a8c\u503c\u653e\u5230\u7ed3\u679c\u8bb0\u5f55\u4e2d\u3002\u8fd9\u6837\u68c0\u67e5\u53c2\u6570\u5dee\u5f02\u65f6\uff0c\u624d\u4e0d\u5bb9\u6613\u628a\u7248\u672c\u53d8\u5316\u8bef\u8ba4\u4e3a\u65b9\u6cd5\u53d8\u5316\u3002<\/p>\n
\u5b9e\u9645\u8ba1\u7b97\u7684\u8ddd\u79bb\u4e0e\u624b\u7b97\u662f\u5426\u76f8\u540c<\/h2>\n
\u672c\u6b21\u5148\u8fd0\u884c\u8ddd\u79bb\u8ba1\u7b97\uff0c\u8f93\u51fa\u4e0b\u4e09\u89d2\u77e9\u9635\uff0c\u4fdd\u7559\u516d\u7ec4\u4e24\u4e24\u6bd4\u8f83\u7ed3\u679c\u3002<\/p>\n
mothur "#dist.seqs(fasta=aligned.fasta,output=lt,processors=2,cutoff=0.2)"\r\ncp aligned.phylip.dist input.phylip.dist\r\n<\/code><\/pre>\n
\u5bf9\u4e8e\u672c\u4f8b\u7684\u7b49\u957f\u3001\u65e0\u7f3a\u53e3\u5e8f\u5217\uff0c\u9010\u4f4d\u6bd4\u8f83\u5f97\u5230\u7684\u5dee\u5f02\u6bd4\u4f8b\u4e0e\u7a0b\u5e8f\u8f93\u51fa\u4e00\u81f4\u3002\u516d\u7ec4\u8ddd\u79bb\u5206\u522b\u4e3a A \u4e0e B \u7684 0.020\u3001A \u4e0e C \u7684 0.045\u3001A \u4e0e D \u7684 0.080\u3001B \u4e0e C \u7684 0.025\u3001B \u4e0e D \u7684 0.100\uff0c\u4ee5\u53ca C \u4e0e D \u7684 0.125\u3002<\/p>\n
\u8fd9\u91cc\u6709\u4e00\u4e2a\u503c\u5f97\u8bb0\u4f4f\u7684\u53c2\u6570\u7ec6\u8282\u3002\u5b98\u65b9\u8bf4\u660e\u4e2d\uff0c\u4e0b\u4e09\u89d2\u6216\u65b9\u9635\u8f93\u51fa\u4f1a\u5ffd\u7565\u8ddd\u79bb\u8ba1\u7b97\u547d\u4ee4\u7684\u622a\u65ad\u9009\u9879\u3002\u56e0\u6b64\uff0c\u672c\u6b21\u4f7f\u7528\u4e0b\u4e09\u89d2\u683c\u5f0f\u65f6\u83b7\u5f97\u5b8c\u6574\u8ddd\u79bb\u77e9\u9635\uff0c\u4e0d\u80fd\u58f0\u79f0\u9760\u540c\u4e00\u6761\u547d\u4ee4\u4e2d\u7684\u622a\u65ad\u503c\u51cf\u5c11\u4e86\u77e9\u9635\u5185\u5bb9\u3002dist.seqs \u53c2\u6570\u8bf4\u660e<\/a><\/p>\n
\u5b9e\u9645\u6269\u589e\u5b50\u6570\u636e\u5e38\u5305\u542b\u5bf9\u9f50\u7f3a\u53e3\u3001\u7aef\u90e8\u957f\u5ea6\u5dee\u5f02\u4ee5\u53ca\u9700\u8981\u8fc7\u6ee4\u7684\u4f4d\u7f6e\u3002\u6b64\u65f6\u8ddd\u79bb\u7684\u5b9a\u4e49\u8fd8\u53d7\u5230\u7f3a\u53e3\u5904\u7406\u4e0e\u6709\u6548\u6bd4\u8f83\u8303\u56f4\u5f71\u54cd\uff0c\u4e0d\u80fd\u7167\u642c\u672c\u4f8b\u201c\u5dee\u5f02\u4f4d\u7f6e\u6570\u9664\u4ee5\u4e00\u5343\u201d\u7684\u7b80\u5316\u7b97\u6cd5\u3002\u5148\u786e\u8ba4\u8f93\u5165\u5df2\u7ecf\u8986\u76d6\u53ef\u6bd4\u8f83\u533a\u57df\uff0c\u518d\u89e3\u91ca\u8ddd\u79bb\u9608\u503c\uff0c\u901a\u5e38\u6bd4\u76f4\u63a5\u8c03\u6574\u805a\u7c7b\u547d\u4ee4\u66f4\u6709\u4ef7\u503c\u3002<\/p>\n
\u6700\u8fd1\u90bb\u4e3a\u4ec0\u4e48\u628a A\u3001B\u3001C \u653e\u5230\u4e00\u8d77<\/h2>\n
\u6211\u4eec\u9996\u5148\u6307\u5b9a\u6700\u8fd1\u90bb\u65b9\u6cd5\uff0c\u89c2\u5bdf\u767e\u5206\u4e4b\u4e09\u8ddd\u79bb\u9644\u8fd1\u7684\u5206\u7ec4\u3002<\/p>\n
mothur "#cluster(phylip=input.phylip.dist,method=nearest,cutoff=0.03)"\r\n<\/code><\/pre>\n
\u5b9e\u9645\u7ed3\u679c\u4e2d\uff0cA \u4e0e B \u5148\u5408\u5e76\u3002\u968f\u540e B \u4e0e C \u7684\u8ddd\u79bb\u4e3a\u767e\u5206\u4e4b\u4e8c\u70b9\u4e94\uff0c\u8db3\u591f\u8fd1\uff0cC \u4e5f\u52a0\u5165\u540c\u4e00\u4e2a\u7c07\u3002\u6700\u7ec8\u5f62\u6210\u5305\u542b A\u3001B\u3001C \u7684\u4e00\u4e2a\u7c07\uff0c\u4ee5\u53ca\u53ea\u5305\u542b D \u7684\u53e6\u4e00\u4e2a\u7c07\u3002<\/p>\n
\u4f46 A \u4e0e C \u7684\u8ddd\u79bb\u4e3a\u767e\u5206\u4e4b\u56db\u70b9\u4e94\uff0c\u5df2\u7ecf\u9ad8\u4e8e\u767e\u5206\u4e4b\u4e09\u3002\u4e8c\u8005\u4ecd\u7136\u540c\u7c07\uff0c\u662f\u56e0\u4e3a\u6700\u8fd1\u90bb\u5141\u8bb8\u901a\u8fc7\u5176\u4ed6\u6210\u5458\u5efa\u7acb\u8fde\u63a5\u3002\u672c\u6b21\u7ed3\u679c\u6070\u597d\u63d0\u4f9b\u4e86\u4e00\u4e2a\u80fd\u591f\u76f4\u63a5\u68c0\u67e5\u7684\u4f8b\u5b50\uff0c\u907f\u514d\u628a\u201c\u540c\u7c07\u201d\u9519\u8bef\u89e3\u91ca\u4e3a\u201c\u4efb\u610f\u4e24\u6761\u90fd\u4f4e\u4e8e\u9608\u503c\u201d\u3002<\/p>\n
\u8fd9\u79cd\u8fde\u63a5\u89c4\u5219\u5bb9\u6613\u7406\u89e3\uff0c\u4e5f\u80fd\u628a\u6cbf\u7740\u5c40\u90e8\u76f8\u4f3c\u5173\u7cfb\u76f8\u8fde\u7684\u5e8f\u5217\u805a\u5230\u4e00\u8d77\u3002\u4f7f\u7528\u65f6\u9700\u8981\u6ce8\u610f\u94fe\u5f0f\u6269\u5c55\u7684\u5f71\u54cd\u3002\u5982\u679c\u7814\u7a76\u95ee\u9898\u8981\u6c42\u6bcf\u5bf9\u6210\u5458\u90fd\u6ee1\u8db3\u76f8\u8fd1\u7a0b\u5ea6\uff0c\u5c31\u5e94\u660e\u786e\u68c0\u67e5\u6240\u7528\u65b9\u6cd5\u662f\u5426\u4fdd\u8bc1\u8fd9\u4e00\u70b9\uff0c\u800c\u4e0d\u662f\u4ec5\u51ed\u9608\u503c\u540d\u79f0\u5224\u65ad\u3002<\/p>\n
\u6700\u8fdc\u90bb\u548c\u5e73\u5747\u90bb\u5c45\u4e3a\u4ec0\u4e48\u4ecd\u7136\u4fdd\u7559\u4e09\u4e2a\u7c07<\/h2>\n
\u6700\u8fdc\u90bb\u6bd4\u8f83\u7c07\u4e4b\u95f4\u6700\u8fdc\u7684\u6210\u5458\u3002A \u4e0e B \u5408\u5e76\u540e\uff0c\u82e5\u628a C \u52a0\u8fdb\u6765\uff0c\u5c31\u5fc5\u987b\u9762\u5bf9 A \u4e0e C \u7684\u767e\u5206\u4e4b\u56db\u70b9\u4e94\u8ddd\u79bb\u3002\u56e0\u6b64\uff0c\u5728\u672c\u6b21\u89c2\u5bdf\u7684\u767e\u5206\u4e4b\u4e09\u6c34\u5e73\uff0cC \u4fdd\u6301\u5355\u72ec\uff0c\u6700\u7ec8\u5206\u7ec4\u4e3a A\u3001B \u4e00\u4e2a\u7c07\uff0cC \u4e00\u4e2a\u7c07\uff0cD \u4e00\u4e2a\u7c07\u3002<\/p>\n
\u5e73\u5747\u90bb\u5c45\u5728\u672c\u4f8b\u4e2d\u4e5f\u7559\u4e0b\u4e09\u4e2a\u7c07\uff0c\u4f46\u539f\u56e0\u9700\u8981\u5355\u72ec\u8ba1\u7b97\u3002A\u3001B \u7ec4\u6210\u7684\u7c07\u4e0e C \u7684\u5e73\u5747\u8ddd\u79bb\uff0c\u662f\u767e\u5206\u4e4b\u56db\u70b9\u4e94\u548c\u767e\u5206\u4e4b\u4e8c\u70b9\u4e94\u7684\u5e73\u5747\u503c\uff0c\u4e5f\u5c31\u662f\u767e\u5206\u4e4b\u4e09\u70b9\u4e94\uff0c\u4ecd\u7136\u9ad8\u4e8e\u76ee\u6807\u6c34\u5e73\u3002<\/p>\n
mothur "#cluster(phylip=input.phylip.dist,method=furthest,cutoff=0.03)"\r\nmothur "#cluster(phylip=input.phylip.dist,method=average,cutoff=0.03)"\r\n<\/code><\/pre>\n
\u6700\u8fdc\u90bb\u7684\u5206\u7ec4\u6761\u4ef6\u8f83\u5bb9\u6613\u7528\u4e8e\u68c0\u67e5\u7c07\u5185\u6700\u5927\u5dee\u5f02\uff0c\u4f46\u4e5f\u53ef\u80fd\u8ba9\u4e00\u4e9b\u901a\u8fc7\u5c40\u90e8\u76f8\u4f3c\u6027\u76f8\u8fde\u7684\u5e8f\u5217\u4fdd\u6301\u5206\u79bb\u3002\u5e73\u5747\u90bb\u5c45\u7efc\u5408\u591a\u4e2a\u6210\u5458\u95f4\u7684\u8ddd\u79bb\uff0c\u7ed3\u679c\u53d7\u7c07\u7ec4\u6210\u5f71\u54cd\u3002\u672c\u6587\u7684\u8ba1\u7b97\u8bf4\u660e\u4e86\u4e09\u79cd\u89c4\u5219\u4e3a\u4f55\u4e0d\u540c\uff0c\u6ca1\u6709\u7ed9\u51fa\u4e00\u79cd\u80fd\u591f\u9002\u7528\u4e8e\u6240\u6709\u6269\u589e\u5b50\u4efb\u52a1\u7684\u4f18\u80dc\u65b9\u6cd5\u3002<\/p>\n
\u53e6\u5916\uff0c\u5f53\u524d\u5b98\u65b9\u6587\u6863\u5217\u51fa\u7684\u9ed8\u8ba4\u65b9\u6cd5\u4e3a OptiClust\u3002\u672c\u6b21\u4e3a\u4e86\u5c55\u793a\u8ddd\u79bb\u8fde\u63a5\u89c4\u5219\uff0c\u660e\u786e\u6307\u5b9a\u4e86\u4e0a\u8ff0\u4e09\u79cd\u65b9\u6cd5\uff0c\u8bfb\u8005\u4e0d\u80fd\u628a\u8fd9\u91cc\u7684\u7ed3\u679c\u5f53\u4f5c\u7701\u7565\u65b9\u6cd5\u53c2\u6570\u65f6\u7684\u9ed8\u8ba4\u8f93\u51fa\u3002cluster \u65b9\u6cd5\u4e0e\u9ed8\u8ba4\u53c2\u6570<\/a><\/p>\n
\u4e3a\u4ec0\u4e48\u8fd8\u8981\u628a\u8ba1\u7b97\u8303\u56f4\u6269\u5927\u540e\u518d\u8dd1\u4e00\u6b21<\/h2>\n
\u521d\u6b21\u5bf9\u7167\u5c06\u805a\u7c7b\u547d\u4ee4\u7684\u622a\u65ad\u503c\u8bbe\u4e3a 0.03\uff0c\u80fd\u76f4\u63a5\u770b\u5230\u672c\u4f8b\u7684\u533a\u522b\u3002\u4f46\u6b63\u5f0f\u6d41\u7a0b\u4e2d\uff0c\u5e94\u533a\u5206\u8ba1\u7b97\u65f6\u4fdd\u7559\u7684\u8ddd\u79bb\u8303\u56f4\u4e0e\u6700\u540e\u51c6\u5907\u8bfb\u53d6\u7684\u5206\u7ec4\u6c34\u5e73\u3002\u5bf9\u4e8e\u8fd9\u91cc\u7684\u4e09\u79cd\u90bb\u5c45\u65b9\u6cd5\uff0c\u5b98\u65b9\u5efa\u8bae\u8ba1\u7b97\u8303\u56f4\u9ad8\u4e8e\u6700\u7ec8\u5173\u6ce8\u7684\u8ddd\u79bb\u3002<\/p>\n
\u4e3a\u68c0\u67e5\u521d\u6b21\u7ed3\u679c\u662f\u5426\u53d7\u5230\u8303\u56f4\u8bbe\u7f6e\u5f71\u54cd\uff0c\u6211\u4eec\u53c8\u628a\u4e09\u79cd\u65b9\u6cd5\u5168\u90e8\u6539\u4e3a 0.2 \u8fd0\u884c\u3002\u5b8c\u6574\u77e9\u9635\u4fdd\u6301\u76f8\u540c\uff0c\u8f93\u5165\u5e8f\u5217\u548c\u65b9\u6cd5\u540d\u79f0\u4e5f\u4fdd\u6301\u76f8\u540c\uff0c\u53ea\u6269\u5927\u8ba1\u7b97\u8303\u56f4\uff0c\u518d\u4ece\u8f93\u51fa\u4e2d\u8bfb\u53d6\u76ee\u6807\u8ddd\u79bb\u9644\u8fd1\u7684\u72b6\u6001\u3002<\/p>\n
\u590d\u73b0\u9a71\u52a8\u4e3a\u6bcf\u4e2a\u65b9\u6cd5\u548c\u8ba1\u7b97\u8303\u56f4\u5efa\u7acb\u72ec\u7acb\u76ee\u5f55\uff0c\u5e76\u628a\u540c\u4e00\u4efd\u77e9\u9635\u590d\u5236\u4e3a\u4e0b\u9762\u547d\u4ee4\u4f7f\u7528\u7684\u6587\u4ef6\u540d\uff0c\u907f\u514d\u4e0d\u540c\u5bf9\u7167\u8986\u76d6\u8f93\u51fa\u3002\u6269\u5927\u8303\u56f4\u7684\u5e73\u5747\u90bb\u5c45\u547d\u4ee4\u5982\u4e0b\uff0c\u53e6\u5916\u4e24\u79cd\u65b9\u6cd5\u53ea\u66ff\u6362\u65b9\u6cd5\u540d\u79f0\u3002<\/p>\n
mothur "#cluster(phylip=input.phylip.dist,method=average,cutoff=0.2)"\r\n<\/code><\/pre>\n
\u6269\u5927\u8303\u56f4\u540e\uff0c\u6700\u8fd1\u90bb\u4f9d\u65e7\u5728\u6807\u7b7e 0.03 \u5bf9\u5e94\u4e24\u4e2a\u7c07\u3002\u5e73\u5747\u90bb\u5c45\u5728\u6807\u7b7e 0.02 \u65f6\u662f\u4e09\u4e2a\u7c07\uff0c\u5230\u6807\u7b7e 0.04 \u624d\u5408\u5e76\u6210\u4e24\u4e2a\u3002\u6700\u8fdc\u90bb\u540c\u6837\u5148\u4fdd\u6301\u4e09\u4e2a\u7c07\uff0c\u5230\u6807\u7b7e 0.05 \u624d\u53d8\u4e3a\u4e24\u4e2a\u3002\u8fd9\u4e9b\u53d8\u5316\u4e0e\u524d\u9762\u72ec\u7acb\u8ba1\u7b97\u7684\u8ddd\u79bb\u5173\u7cfb\u76f8\u7b26\u3002<\/p>\n
\u672c\u6b21\u8f93\u51fa\u4fdd\u7559\u4e86\u9ed8\u8ba4\u7cbe\u5ea6\u4e0b\u7684\u6807\u7b7e\uff0c\u6240\u4ee5\u767e\u5206\u4e4b\u4e09\u70b9\u4e94\u548c\u767e\u5206\u4e4b\u56db\u70b9\u4e94\u5bf9\u5e94\u7684\u53d8\u5316\uff0c\u5206\u522b\u663e\u793a\u5728 0.04 \u548c 0.05 \u884c\u3002\u5e94\u4fdd\u7559\u539f\u59cb\u8ddd\u79bb\u548c\u8f93\u51fa\u6807\u7b7e\u4e24\u4efd\u8bc1\u636e\uff0c\u4e0d\u8981\u628a\u6253\u5370\u6807\u7b7e\u7684\u4f4d\u6570\u5f53\u4f5c\u539f\u59cb\u8ddd\u79bb\u7684\u5168\u90e8\u7cbe\u5ea6\u3002<\/p>\n
\u6307\u5b9a\u8bfb\u53d6 0.03\uff0c\u6587\u4ef6\u91cc\u4e3a\u4ec0\u4e48\u51fa\u73b0 0.02<\/h2>\n
\u8fdb\u4e00\u6b65\u8bfb\u53d6\u7ed3\u679c\u65f6\uff0c\u6211\u4eec\u4e3a\u56db\u6761\u5e8f\u5217\u5efa\u7acb\u540c\u4e00\u4e2a\u6f14\u793a\u6837\u672c\u7684\u5f52\u5c5e\u6587\u4ef6\uff0c\u518d\u8fd0\u884c\u4e30\u5ea6\u8868\u751f\u6210\u547d\u4ee4\u3002\u8fd9\u6837\u53ef\u4ee5\u540c\u65f6\u68c0\u67e5\u5206\u7ec4\u6570\u91cf\u548c\u6bcf\u4e2a\u5206\u7ec4\u4e2d\u5305\u542b\u7684\u5e8f\u5217\u6570\u91cf\u3002<\/p>\n
printf 'A\\tdemo\\nB\\tdemo\\nC\\tdemo\\nD\\tdemo\\n' > input.groups\r\nmothur "#make.shared(list=input.phylip.an.list,group=input.groups,label=0.03)"\r\n<\/code><\/pre>\n
\u5e73\u5747\u90bb\u5c45\u7684\u5217\u8868\u4e2d\u6ca1\u6709\u5355\u72ec\u7684 0.03 \u884c\uff0c\u56e0\u4e3a\u5728\u8be5\u4f4d\u7f6e\u4e4b\u524d\uff0c\u5206\u7ec4\u4ecd\u7136\u4fdd\u6301 A\u3001B \u5408\u5e76\u800c C\u3001D \u72ec\u7acb\u7684\u72b6\u6001\u3002\u5b9e\u9645\u751f\u6210\u7684\u4e30\u5ea6\u8868\u4f7f\u7528\u4e86 0.02 \u6807\u7b7e\uff0c\u4e09\u4e2a\u5206\u7ec4\u7684\u8ba1\u6570\u5206\u522b\u4e3a\u4e8c\u3001\u4e00\u3001\u4e00\u3002<\/p>\n
\u6700\u8fdc\u90bb\u540c\u6837\u8fd4\u56de 0.02 \u6807\u7b7e\u4e0e\u4e09\u4e2a\u5206\u7ec4\u3002\u6700\u8fd1\u90bb\u5219\u8fd4\u56de 0.03 \u6807\u7b7e\uff0c\u4e24\u4e2a\u5206\u7ec4\u7684\u8ba1\u6570\u4e3a\u4e09\u548c\u4e00\u3002\u8fd9\u4e9b\u90fd\u662f\u7a0b\u5e8f\u5b9e\u9645\u5199\u51fa\u7684\u7ed3\u679c\uff0c\u6ca1\u6709\u4e3a\u4e86\u8ba9\u8868\u683c\u6574\u9f50\u800c\u5c06\u6807\u7b7e\u624b\u52a8\u6539\u6210\u76f8\u540c\u6570\u5b57\u3002make.shared \u8f93\u5165\u4e0e\u6807\u7b7e\u9009\u62e9<\/a><\/p>\n
\n\n\n\n\n\n\n\n
\u65b9\u6cd5<\/th>\n\u8bf7\u6c42\u6807\u7b7e<\/th>\n\u8f93\u51fa\u6807\u7b7e<\/th>\n\u5206\u7ec4\u6570<\/th>\n\u6210\u5458\u5173\u7cfb<\/th>\n<\/tr>\n<\/thead>\n
\u6700\u8fd1\u90bb<\/td>\n0.03<\/td>\n0.03<\/td>\n2<\/td>\nA\u3001B\u3001C \u540c\u7ec4\uff0cD \u72ec\u7acb<\/td>\n<\/tr>\n
\u6700\u8fdc\u90bb<\/td>\n0.03<\/td>\n0.02<\/td>\n3<\/td>\nA\u3001B \u540c\u7ec4\uff0cC \u4e0e D \u5404\u81ea\u72ec\u7acb<\/td>\n<\/tr>\n
\u5e73\u5747\u90bb\u5c45<\/td>\n0.03<\/td>\n0.02<\/td>\n3<\/td>\nA\u3001B \u540c\u7ec4\uff0cC \u4e0e D \u5404\u81ea\u72ec\u7acb<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u6392\u67e5\u81ea\u5df1\u7684\u8f93\u51fa\u65f6\uff0c\u5148\u67e5\u770b\u5217\u8868\u4e2d\u5b9e\u9645\u6709\u54ea\u4e9b\u6807\u7b7e\uff0c\u518d\u6838\u5bf9\u8bfb\u53d6\u547d\u4ee4\u548c\u65e5\u5fd7\u3002\u67d0\u4e00\u884c\u6ca1\u6709\u51fa\u73b0\uff0c\u5e76\u4e0d\u81ea\u52a8\u4ee3\u8868\u7a0b\u5e8f\u6f0f\u7b97\u6216\u8fd0\u884c\u5931\u8d25\u3002\u672c\u6b21\u901a\u8fc7\u6269\u5927\u8303\u56f4\u548c\u5b9e\u9645\u751f\u6210\u4e30\u5ea6\u8868\uff0c\u786e\u8ba4\u4e86\u76ee\u6807\u6c34\u5e73\u5bf9\u5e94\u7684\u5206\u7ec4\u72b6\u6001\u3002<\/p>\n
\u600e\u6837\u6bd4\u8f83\u4e24\u4efd\u771f\u5b9e\u9879\u76ee\u7ed3\u679c<\/h2>\n
\u62ff\u5230\u7ed3\u679c\u540e\uff0c\u7b2c\u4e00\u6b65\u53ef\u4ee5\u68c0\u67e5\u4ee3\u8868\u5e8f\u5217\u662f\u5426\u76f8\u540c\u3002\u5982\u679c\u8f93\u5165\u672c\u8eab\u7ecf\u8fc7\u4e0d\u540c\u7684\u8d28\u91cf\u8fc7\u6ee4\u3001\u53bb\u5d4c\u5408\u4f53\u6216\u53bb\u5197\u4f59\u5904\u7406\uff0c\u5373\u4f7f\u540e\u7eed\u65b9\u6cd5\u4e0e\u9608\u503c\u76f8\u540c\uff0c\u4e5f\u53ef\u80fd\u5f97\u5230\u4e0d\u540c\u6570\u91cf\u3002\u6b64\u65f6\u53ea\u6bd4\u8f83\u6700\u7ec8 OTU \u6570\uff0c\u96be\u4ee5\u627e\u5230\u5dee\u5f02\u6765\u81ea\u54ea\u91cc\u3002<\/p>\n
\u7b2c\u4e8c\u6b65\u6838\u5bf9\u8ddd\u79bb\u77e9\u9635\u7684\u5b9a\u4e49\u3002\u786e\u8ba4\u5bf9\u9f50\u8303\u56f4\u3001\u7f3a\u53e3\u5904\u7406\u548c\u4f7f\u7528\u7684\u5e8f\u5217\u96c6\u5408\uff0c\u5c24\u5176\u6ce8\u610f\u662f\u5426\u5c06\u4e0d\u540c\u6807\u8bb0\u533a\u57df\u6216\u957f\u5ea6\u5dee\u5f02\u5f88\u5927\u7684\u7247\u6bb5\u76f4\u63a5\u653e\u5230\u4e00\u8d77\u3002\u8ddd\u79bb\u5b9a\u4e49\u4e0d\u540c\uff0c\u540e\u9762\u7684\u9608\u503c\u5c31\u4e0d\u5177\u6709\u5b8c\u5168\u76f8\u540c\u7684\u542b\u4e49\u3002<\/p>\n
\u7b2c\u4e09\u6b65\u68c0\u67e5\u805a\u7c7b\u65b9\u6cd5\u548c\u8ba1\u7b97\u8303\u56f4\u3002\u672c\u6b21\u4e09\u79cd\u65b9\u6cd5\u5df2\u7ecf\u8bf4\u660e\uff0c\u540c\u4e00\u4e2a\u76ee\u6807\u8ddd\u79bb\u65e0\u6cd5\u552f\u4e00\u786e\u5b9a\u5206\u7ec4\u3002\u5bf9\u7167\u5b9e\u9a8c\u5e94\u5148\u56fa\u5b9a\u8f93\u5165\u548c\u8ddd\u79bb\u77e9\u9635\uff0c\u9010\u9879\u6539\u53d8\u65b9\u6cd5\uff0c\u518d\u68c0\u67e5\u54ea\u4e9b\u6210\u5458\u53d1\u751f\u79fb\u52a8\uff0c\u624d\u5bb9\u6613\u7406\u89e3\u53d8\u5316\u673a\u5236\u3002<\/p>\n
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