{"id":72774,"date":"2026-11-15T10:44:25","date_gmt":"2026-11-15T02:44:25","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72774"},"modified":"2026-11-15T10:47:23","modified_gmt":"2026-11-15T02:47:23","slug":"vsearch-dereplication-sizein-strand-abundance-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/vsearch-dereplication-sizein-strand-abundance-practice\/","title":{"rendered":"VSEARCH \u53bb\u5197\u4f59\u540e\u4e30\u5ea6\u4e3a\u4f55\u4ece 28 \u53d8\u6210 6\uff1f\u5b9e\u8dd1\u770b\u61c2 sizein\u3001\u94fe\u65b9\u5411\u4e0e\u4f4e\u4e30\u5ea6\u8fc7\u6ee4"},"content":{"rendered":"
\n

\u6269\u589e\u5b50\u6d41\u7a0b\u4e2d\uff0c\u53bb\u5197\u4f59\u901a\u5e38\u88ab\u770b\u4f5c\u4e00\u4e2a\u7b80\u5355\u6b65\u9aa4\u3002\u76f8\u540c\u5e8f\u5217\u5408\u5728\u4e00\u8d77\uff0c\u6587\u4ef6\u53d8\u5c0f\uff0c\u540e\u9762\u7ee7\u7eed\u5206\u6790\u3002\u4f46\u5982\u679c\u8f93\u5165\u5df2\u7ecf\u5e26\u6709\u4e30\u5ea6\u4fe1\u606f\uff0c\u800c\u547d\u4ee4\u6ca1\u6709\u6309\u9884\u671f\u8bfb\u53d6\u8fd9\u4e9b\u4fe1\u606f\uff0c\u8f93\u51fa\u53ef\u80fd\u770b\u8d77\u6765\u5f88\u6b63\u5e38\uff0c\u8ba1\u6570\u542b\u4e49\u5374\u5df2\u7ecf\u6539\u53d8\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u8fd0\u884c VSEARCH 2.30.4\uff0c\u4f7f\u7528\u516d\u6761\u8bbe\u8ba1\u5e8f\u5217\u5b8c\u6210\u516d\u7ec4\u5bf9\u7167\u3002\u6211\u4eec\u68c0\u67e5\u6b63\u53cd\u94fe\u3001\u5927\u5c0f\u5199\u3001\u8f93\u5165\u4e30\u5ea6\u3001\u8f93\u51fa\u4e30\u5ea6\u548c\u4f4e\u4e30\u5ea6\u8fc7\u6ee4\uff0c\u5e76\u5c06\u4f20\u7edf\u547d\u4ee4\u4e0e\u65b0\u7248\u6587\u6863\u5efa\u8bae\u7684\u5165\u53e3\u653e\u5728\u540c\u4e00\u7ec4\u6570\u636e\u4e0a\u590d\u6838\u3002\u91cd\u70b9\u662f\u8ba9\u8bfb\u8005\u80fd\u591f\u5224\u65ad\u6570\u91cf\u53d8\u5316\u53d1\u751f\u5728\u54ea\u4e00\u5c42\uff0c\u907f\u514d\u628a\u8ba1\u6570\u89c4\u5219\u53d8\u5316\u8bef\u8ba4\u4e3a\u8d28\u91cf\u8fc7\u6ee4\u6548\u679c\u3002<\/p>\n

\"\u4e30\u5ea6\u4ece\u4e8c\u5341\u516b\u53d8\u6210\u516d\u3002\u516d\u7ec4\u5b9e\u8dd1\u6838\u5bf9\u53bb\u5197\u4f59\u7684\u8ba1\u6570\u542b\u4e49\u3002\u8bfb\u53d6\u5df2\u6709\u4e30\u5ea6\uff0c\u603b\u91cf\u4e8c\u5341\u516b\uff0c\u53cc\u94fe\u5408\u5e76\u540e\u4e3a\u4e8c\u5341\u4e94\u3001\u4e8c\u3001\u4e00\u3002\u6309\u8f93\u5165\u8bb0\u5f55\u8ba1\u6570\uff0c\u603b\u91cf\u53d8\u6210\u516d\uff0c\u4ee3\u8868\u8bb0\u5f55\u7684\u6743\u91cd\u6ca1\u6709\u88ab\u7d2f\u52a0\u3002\u518d\u8fc7\u6ee4\u4f4e\u4e30\u5ea6\uff0c\u8ba1\u6570\u4ecd\u987b\u6838\u5bf9\uff0c\u6700\u7ec8\u79cd\u7c7b\u76f8\u540c\uff0c\u4e30\u5ea6\u53ef\u80fd\u4e0d\u540c\u3002\"
\u516d\u7ec4\u5b9e\u8dd1\u6838\u5bf9\u53bb\u5197\u4f59\u7684\u8ba1\u6570\u542b\u4e49<\/figcaption><\/figure>\n

\u5148\u786e\u8ba4\u81ea\u5df1\u60f3\u5408\u5e76\u7684\u662f\u4ec0\u4e48<\/h2>\n

\u5168\u957f\u53bb\u5197\u4f59\u9488\u5bf9\u7684\u662f\u5b8c\u5168\u76f8\u540c\u7684\u5e8f\u5217\u3002\u5b83\u80fd\u591f\u628a\u91cd\u590d\u8bb0\u5f55\u6536\u62e2\u4e3a\u4ee3\u8868\u5e8f\u5217\uff0c\u540c\u65f6\u4fdd\u5b58\u5bf9\u5e94\u8ba1\u6570\u3002\u5b83\u4e0e\u6309\u76f8\u4f3c\u5ea6\u805a\u7c7b\u3001\u6d4b\u5e8f\u9519\u8bef\u6821\u6b63\u548c\u53bb\u5d4c\u5408\u4f53\u5c5e\u4e8e\u4e0d\u540c\u64cd\u4f5c\uff0c\u4e0d\u80fd\u56e0\u4e3a\u8f93\u51fa\u8bb0\u5f55\u53d8\u5c11\uff0c\u5c31\u628a\u5b83\u4eec\u90fd\u79f0\u4e3a\u540c\u4e00\u79cd\u6e05\u7406\u3002<\/p>\n

\u8fd9\u91cc\u81f3\u5c11\u9700\u8981\u533a\u5206\u4e24\u4e2a\u6570\u91cf\u3002\u4e00\u4e2a\u662f\u6587\u4ef6\u4e2d\u7684\u5e8f\u5217\u8bb0\u5f55\u6570\uff0c\u53e6\u4e00\u4e2a\u662f\u8fd9\u4e9b\u8bb0\u5f55\u6240\u4ee3\u8868\u7684\u603b\u4e30\u5ea6\u3002\u5f53\u8f93\u5165\u5df2\u7ecf\u53bb\u8fc7\u4e00\u6b21\u5197\u4f59\uff0c\u6bcf\u6761\u8bb0\u5f55\u53ef\u80fd\u4ee3\u8868\u591a\u6b21\u89c2\u6d4b\uff0c\u4e8c\u8005\u901a\u5e38\u4e0d\u518d\u76f8\u540c\u3002<\/p>\n

\u672c\u4f8b\u6545\u610f\u8ba9\u516d\u6761\u8f93\u5165\u8bb0\u5f55\u4ee3\u8868\u4e8c\u5341\u516b\u6b21\u89c2\u6d4b\u3002\u8fd9\u6837\u5c31\u80fd\u7528\u5f88\u5c0f\u7684\u6570\u636e\u770b\u6e05\u7a0b\u5e8f\u5230\u5e95\u5728\u7d2f\u52a0\u5df2\u6709\u4e30\u5ea6\uff0c\u8fd8\u662f\u91cd\u65b0\u6309\u6bcf\u6761\u8bb0\u5f55\u4e00\u6b21\u8ba1\u6570\u3002\u6ca1\u6709\u660e\u786e\u8ba1\u6570\u5355\u4f4d\u4e4b\u524d\uff0c\u8ba8\u8bba\u4fdd\u7559\u7387\u548c\u7a00\u6709\u7279\u5f81\u90fd\u5bb9\u6613\u51fa\u73b0\u6b67\u4e49\u3002<\/p>\n

\u672c\u6b21\u8f93\u5165\u5982\u4f55\u6784\u9020<\/h2>\n

\u9a71\u52a8\u811a\u672c\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\uff0c\u751f\u6210\u4e00\u6761\u4e09\u767e\u78b1\u57fa\u7684\u57fa\u7840\u5e8f\u5217\u3002\u7b2c\u4e00\u6761\u8bb0\u5f55\u4fdd\u5b58\u5b83\uff0c\u4e30\u5ea6\u8bbe\u4e3a\u5341\u3002\u7b2c\u4e8c\u6761\u8bb0\u5f55\u4e0e\u5b83\u5b8c\u5168\u76f8\u540c\uff0c\u4e30\u5ea6\u4e3a\u4e94\u3002\u7b2c\u4e09\u6761\u8bb0\u5f55\u662f\u5b83\u7684\u53cd\u5411\u4e92\u8865\u5e8f\u5217\uff0c\u4e30\u5ea6\u4e3a\u4e09\u3002<\/p>\n

\u7b2c\u56db\u6761\u8bb0\u5f55\u4ecd\u7136\u662f\u57fa\u7840\u5e8f\u5217\uff0c\u4f46\u5168\u90e8\u5199\u6210\u5c0f\u5199\uff0c\u4e30\u5ea6\u8bbe\u4e3a\u4e03\u3002\u7b2c\u4e94\u6761\u53ea\u6539\u53d8\u4e00\u4e2a\u78b1\u57fa\uff0c\u4e30\u5ea6\u4e3a\u4e8c\u3002\u7b2c\u516d\u6761\u662f\u72ec\u7acb\u751f\u6210\u7684\u53e6\u4e00\u6761\u5e8f\u5217\uff0c\u4e30\u5ea6\u4e3a\u4e00\u3002\u516d\u4e2a\u4e30\u5ea6\u76f8\u52a0\u5f97\u5230\u4e8c\u5341\u516b\uff0c\u800c\u6587\u4ef6\u4e2d\u786e\u5b9e\u53ea\u6709\u516d\u4e2a\u8bb0\u5f55\u5934\u3002<\/p>\n

\u8fd9\u4e9b\u4e30\u5ea6\u5199\u5728 FASTA \u6807\u9898\u7684\u6807\u51c6\u6ce8\u91ca\u4f4d\u7f6e\uff0c\u590d\u73b0\u5305\u4f1a\u751f\u6210\u5b8c\u6574\u78b1\u57fa\u4e32\u5e76\u8bb0\u5f55\u6821\u9a8c\u503c\u3002\u4e0b\u9762\u53ea\u793a\u610f\u6807\u9898\uff0c\u5b9e\u9645\u5b9e\u9a8c\u6ca1\u6709\u4f7f\u7528\u7701\u7565\u540e\u7684\u5e8f\u5217\u3002<\/p>\n

>forward1;size=10;\r\n>forward2;size=5;\r\n>reverse;size=3;\r\n>lowercase;size=7;\r\n>variant;size=2;\r\n>unrelated;size=1;\r\n<\/code><\/pre>\n
\u8fd9\u79cd\u8f93\u5165\u540c\u65f6\u63d0\u4f9b\u51e0\u7c7b\u53ef\u68c0\u67e5\u5173\u7cfb\u3002\u5b8c\u5168\u76f8\u540c\u7684\u4e24\u6761\u8bb0\u5f55\u53ef\u4ee5\u68c0\u67e5\u57fa\u672c\u5408\u5e76\uff0c\u5c0f\u5199\u8bb0\u5f55\u53ef\u4ee5\u68c0\u67e5\u5927\u5c0f\u5199\u5904\u7406\uff0c\u53cd\u5411\u4e92\u8865\u8bb0\u5f55\u53ef\u4ee5\u68c0\u67e5\u65b9\u5411\u9009\u9879\uff0c\u5355\u78b1\u57fa\u53d8\u4f53\u5219\u7528\u4e8e\u68c0\u67e5\u662f\u5426\u53d1\u751f\u4e86\u8d85\u51fa\u5168\u957f\u5b8c\u5168\u76f8\u540c\u6761\u4ef6\u7684\u5408\u5e76\u3002<\/p>\n
\u8fd0\u884c\u73af\u5883\u4e0e\u547d\u4ee4\u7248\u672c<\/h2>\n
\u672c\u6b21\u4f7f\u7528\u5b89\u88c5\u7684\u5b98\u65b9 QIIME 2 \u53d1\u884c\u73af\u5883\u4e2d\u63d0\u4f9b\u7684 VSEARCH\uff0c\u5b9e\u9645\u53ef\u6267\u884c\u6587\u4ef6\u7248\u672c\u4e3a 2.30.4\u3002\u6bcf\u6b21\u5b9e\u9a8c\u90fd\u76f4\u63a5\u8c03\u7528\u547d\u4ee4\u884c\u7a0b\u5e8f\uff0c\u9a71\u52a8\u4fdd\u5b58\u7248\u672c\u3001\u6587\u4ef6\u6821\u9a8c\u503c\u3001\u8f93\u5165\u4fe1\u606f\u548c\u6bcf\u7ec4\u8f93\u51fa\u3002VSEARCH \u5b98\u65b9\u9879\u76ee<\/a><\/p>\n
vsearch --version\r\npython run.py result\r\n<\/code><\/pre>\n
\u590d\u73b0\u5305\u4e2d\u63d0\u4f9b\u5b8c\u6574\u73af\u5883\u83b7\u53d6\u65b9\u5f0f\u548c\u955c\u50cf\u6458\u8981\u3002\u5982\u679c\u5df2\u6709\u76f8\u540c\u7248\u672c\u7684\u53ef\u6267\u884c\u7a0b\u5e8f\uff0c\u4e5f\u53ef\u4ee5\u76f4\u63a5\u5728\u80fd\u591f\u8fd0\u884c Python \u7684\u73af\u5883\u4e2d\u6267\u884c\u9a71\u52a8\u3002\u5148\u6838\u5bf9\u7248\u672c\u6709\u52a9\u4e8e\u533a\u5206\u73af\u5883\u6539\u53d8\u4e0e\u53c2\u6570\u6539\u53d8\uff0c\u4e0d\u5fc5\u4e3a\u4e86\u590d\u73b0\u5c0f\u5b9e\u9a8c\u91cd\u65b0\u5efa\u7acb\u5b8c\u6574\u6269\u589e\u5b50\u6d41\u7a0b\u3002<\/p>\n
\u5728\u7ebf\u6587\u6863\u4f1a\u968f\u7248\u672c\u66f4\u65b0\u3002\u672c\u6587\u5b9e\u8dd1\u56fa\u5b9a\u5728\u4e0a\u8ff0\u7248\u672c\uff0c\u540e\u9762\u6d89\u53ca\u65b0\u7248\u6587\u6863\u5efa\u8bae\u65f6\u5355\u72ec\u8bf4\u660e\uff0c\u5e76\u989d\u5916\u9a8c\u8bc1\u76f8\u5173\u547d\u4ee4\u5728\u672c\u6b21\u73af\u5883\u4e2d\u7684\u5b9e\u9645\u8f93\u51fa\uff0c\u907f\u514d\u628a\u4e0d\u540c\u7248\u672c\u7684\u8bf4\u660e\u65e0\u6761\u4ef6\u6df7\u7528\u3002<\/p>\n
\u7b2c\u4e00\u7ec4\u53ea\u6bd4\u8f83\u6b63\u5411\uff0c\u4e3a\u4ec0\u4e48\u5408\u5e76\u6210\u56db\u6761<\/h2>\n
\u7b2c\u4e00\u7ec4\u660e\u786e\u8bfb\u53d6\u8f93\u5165\u4e30\u5ea6\u3001\u8f93\u51fa\u5408\u5e76\u540e\u7684\u4e30\u5ea6\uff0c\u5e76\u53ea\u6bd4\u8f83\u6b63\u5411\u3002<\/p>\n
vsearch --derep_fulllength input.fna \\\r\n  --output plus_sizein.fna \\\r\n  --uc plus_sizein.uc \\\r\n  --strand plus \\\r\n  --sizein --sizeout \\\r\n  --minuniquesize 1\r\n<\/code><\/pre>\n
\u5b9e\u9645\u8f93\u51fa\u56db\u6761\u4ee3\u8868\u5e8f\u5217\uff0c\u603b\u4e30\u5ea6\u4ecd\u7136\u4e3a\u4e8c\u5341\u516b\u3002\u5176\u4e2d\u57fa\u7840\u5e8f\u5217\u5bf9\u5e94\u7684\u4e30\u5ea6\u53d8\u6210\u4e8c\u5341\u4e8c\uff0c\u7b49\u4e8e\u5341\u3001\u4e94\u548c\u4e03\u76f8\u52a0\u3002\u8bf4\u660e\u5b8c\u5168\u76f8\u540c\u7684\u4e24\u6761\u8bb0\u5f55\u4e0e\u5c0f\u5199\u8bb0\u5f55\u5408\u5e76\u5230\u4e86\u4e00\u8d77\u3002<\/p>\n
\u53cd\u5411\u4e92\u8865\u8bb0\u5f55\u4fdd\u7559\u4e3a\u5355\u72ec\u4e00\u6761\uff0c\u4e30\u5ea6\u4e3a\u4e09\u3002\u5355\u78b1\u57fa\u53d8\u4f53\u4e0e\u53e6\u4e00\u6761\u72ec\u7acb\u5e8f\u5217\u4e5f\u5404\u81ea\u4fdd\u7559\uff0c\u4e30\u5ea6\u5206\u522b\u4e3a\u4e8c\u548c\u4e00\u3002\u5bf9\u4e8e\u8fd9\u4e00\u7ec4\uff0c\u5e8f\u5217\u8bb0\u5f55\u6570\u51cf\u5c11\u4e86\uff0c\u4f46\u603b\u4e30\u5ea6\u6ca1\u6709\u51cf\u5c11\u3002<\/p>\n
\u8fd9\u4e00\u6b65\u53ef\u4ee5\u5e2e\u52a9\u6392\u67e5\u4e00\u4e2a\u5e38\u89c1\u8bef\u4f1a\u3002\u53bb\u5197\u4f59\u540e\u6587\u4ef6\u4ece\u516d\u6761\u53d8\u6210\u56db\u6761\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u5220\u9664\u4e86\u4e24\u6b21\u89c2\u6d4b\u3002\u91cd\u590d\u8bb0\u5f55\u7684\u8ba1\u6570\u88ab\u6c47\u603b\u5230\u4ee3\u8868\u5e8f\u5217\u4e2d\uff0c\u539f\u6765\u7684\u89c2\u6d4b\u603b\u91cf\u4ecd\u80fd\u4ece\u8f93\u51fa\u4e30\u5ea6\u4e2d\u6062\u590d\u3002<\/p>\n
\u7b2c\u4e8c\u7ec4\u8003\u8651\u53cc\u94fe\uff0c\u54ea\u4e00\u6761\u8bb0\u5f55\u7ee7\u7eed\u5408\u5e76<\/h2>\n
\u7b2c\u4e8c\u7ec4\u53ea\u628a\u94fe\u65b9\u5411\u6539\u4e3a\u53cc\u94fe\uff0c\u5176\u4f59\u6761\u4ef6\u4e0d\u53d8\u3002\u5b9e\u9645\u8f93\u51fa\u4e09\u6761\u4ee3\u8868\u5e8f\u5217\uff0c\u603b\u4e30\u5ea6\u4ecd\u7136\u4e3a\u4e8c\u5341\u516b\u3002<\/p>\n
vsearch --derep_fulllength input.fna \\\r\n  --output both_sizein.fna \\\r\n  --uc both_sizein.uc \\\r\n  --strand both \\\r\n  --sizein --sizeout \\\r\n  --minuniquesize 1\r\n<\/code><\/pre>\n
\u6b64\u65f6\u53cd\u5411\u4e92\u8865\u8bb0\u5f55\u4e5f\u5e76\u5165\u57fa\u7840\u5e8f\u5217\uff0c\u4ee3\u8868\u4e30\u5ea6\u4ece\u4e8c\u5341\u4e8c\u589e\u52a0\u5230\u4e8c\u5341\u4e94\u3002\u5355\u78b1\u57fa\u53d8\u4f53\u4ecd\u7136\u662f\u4e8c\uff0c\u53e6\u4e00\u6761\u5e8f\u5217\u4ecd\u7136\u662f\u4e00\u3002\u65b9\u5411\u6539\u53d8\u5f71\u54cd\u4e86\u54ea\u4e9b\u8bb0\u5f55\u88ab\u89c6\u4e3a\u540c\u4e00\u5bf9\u8c61\uff0c\u4f46\u5728\u8bfb\u53d6\u4e30\u5ea6\u4e14\u4e0d\u505a\u4f4e\u4e30\u5ea6\u7b5b\u9009\u65f6\uff0c\u6ca1\u6709\u6539\u53d8\u4e30\u5ea6\u603b\u548c\u3002<\/p>\n
\u65b9\u5411\u53c2\u6570\u5e94\u4e0e\u5206\u6790\u76ee\u7684\u76f8\u7b26\u3002\u82e5\u7814\u7a76\u4e2d\u540c\u4e00\u7247\u6bb5\u7684\u4e24\u79cd\u65b9\u5411\u5e94\u5408\u5e76\uff0c\u53ef\u4ee5\u8003\u8651\u8fd9\u79cd\u5904\u7406\u3002\u5982\u679c\u524d\u9762\u5df2\u7ecf\u7edf\u4e00\u4e86\u65b9\u5411\uff0c\u5219\u5e94\u68c0\u67e5\u4e3a\u4f55\u8fd8\u4f1a\u51fa\u73b0\u5927\u91cf\u53cd\u5411\u4e92\u8865\u8bb0\u5f55\u3002\u628a\u65b9\u5411\u9009\u9879\u5199\u660e\uff0c\u6709\u52a9\u4e8e\u5728\u4e0d\u540c\u6d41\u7a0b\u4e4b\u95f4\u6bd4\u8f83\u7ed3\u679c\u3002<\/p>\n
\u672c\u4f8b\u7684\u5c0f\u5199\u8bb0\u5f55\u80fd\u591f\u5408\u5e76\uff0c\u5e76\u4e0d\u8868\u793a\u6240\u6709\u5b57\u7b26\u5dee\u5f02\u90fd\u53ef\u4ee5\u5ffd\u7565\u3002\u5355\u78b1\u57fa\u53d8\u4f53\u4f9d\u7136\u72ec\u7acb\uff0c\u8bf4\u660e\u5168\u957f\u53bb\u5197\u4f59\u6ca1\u6709\u628a\u4e00\u4e2a\u771f\u5b9e\u5b57\u7b26\u4e32\u5dee\u5f02\u81ea\u52a8\u5f53\u4f5c\u6d4b\u5e8f\u9519\u8bef\u6539\u6389\u3002\u662f\u5426\u9700\u8981\u8fdb\u4e00\u6b65\u53bb\u566a\uff0c\u5e94\u7531\u540e\u7eed\u65b9\u6cd5\u548c\u6570\u636e\u8bc1\u636e\u5224\u65ad\u3002<\/p>\n
\u4e0d\u8bfb\u53d6\u8f93\u5165\u4e30\u5ea6\uff0c\u4e3a\u4ec0\u4e48\u603b\u6570\u53d8\u6210\u516d<\/h2>\n
\u7b2c\u4e09\u7ec4\u4fdd\u7559\u53cc\u94fe\u6bd4\u8f83\u548c\u8f93\u51fa\u4e30\u5ea6\uff0c\u4f46\u53bb\u6389\u8bfb\u53d6\u8f93\u5165\u4e30\u5ea6\u7684\u9009\u9879\u3002\u7a0b\u5e8f\u6b63\u5e38\u7ed3\u675f\uff0c\u4ecd\u8f93\u51fa\u4e09\u6761\u4ee3\u8868\u5e8f\u5217\uff0c\u603b\u4e30\u5ea6\u5374\u53d8\u6210\u516d\u3002<\/p>\n
\u57fa\u7840\u5e8f\u5217\u5bf9\u5e94\u56db\u6761\u539f\u59cb\u8bb0\u5f55\uff0c\u56e0\u6b64\u8f93\u51fa\u4e30\u5ea6\u4e3a\u56db\u3002\u5355\u78b1\u57fa\u53d8\u4f53\u548c\u53e6\u4e00\u6761\u5e8f\u5217\u5404\u5bf9\u5e94\u4e00\u6761\u8bb0\u5f55\uff0c\u6240\u4ee5\u90fd\u53d8\u6210\u4e00\u3002\u8fd9\u7ec4\u516d\u6b63\u597d\u7b49\u4e8e\u8f93\u5165\u6587\u4ef6\u7684\u8bb0\u5f55\u6570\uff0c\u800c\u4e0d\u662f\u6807\u9898\u4e2d\u5df2\u6709\u4e30\u5ea6\u7684\u603b\u548c\u3002<\/p>\n
\u8fd9\u91cc\u7684\u53d8\u5316\u4e0d\u662f\u7a0b\u5e8f\u968f\u673a\u4e22\u4e86\u4e8c\u5341\u4e8c\u6761\u8bfb\u6bb5\uff0c\u4e5f\u4e0d\u662f\u78b1\u57fa\u8d28\u91cf\u53d8\u5dee\u3002\u8f93\u5165\u89e3\u91ca\u53d1\u751f\u4e86\u6539\u53d8\uff0c\u6bcf\u6761\u8bb0\u5f55\u88ab\u91cd\u65b0\u5f53\u4f5c\u4e00\u6b21\u89c2\u6d4b\u3002\u8f93\u51fa\u4e30\u5ea6\u9009\u9879\u8d1f\u8d23\u628a\u5f53\u524d\u8ba1\u6570\u5199\u51fa\u6765\uff0c\u5e76\u4e0d\u4f1a\u81ea\u52a8\u4fdd\u8bc1\u8fd9\u4e9b\u8ba1\u6570\u4f7f\u7528\u4e86\u8f93\u5165\u6807\u9898\u4e2d\u7684\u6743\u91cd\u3002<\/p>\n
\u5b9e\u9645\u6570\u636e\u4e2d\uff0c\u8fd9\u79cd\u9519\u8bef\u5c24\u5176\u5bb9\u6613\u53d1\u751f\u5728\u591a\u6b21\u53bb\u5197\u4f59\u6216\u8de8\u6587\u4ef6\u5408\u5e76\u65f6\u3002\u4e0a\u4e00\u6b65\u5df2\u7ecf\u628a\u51e0\u5343\u6761\u8bfb\u6bb5\u538b\u6210\u51e0\u5341\u6761\u4ee3\u8868\u5e8f\u5217\uff0c\u4e0b\u4e00\u6b65\u82e5\u91cd\u65b0\u6309\u4ee3\u8868\u5e8f\u5217\u5404\u4e00\u6b21\u8ba1\u6570\uff0c\u540e\u9762\u6240\u6709\u76f8\u5bf9\u4e30\u5ea6\u8ba1\u7b97\u90fd\u53ef\u80fd\u57fa\u4e8e\u9519\u8bef\u7684\u5206\u6bcd\u3002<\/p>\n
\u56e0\u6b64\uff0c\u68c0\u67e5\u4e00\u4e2a\u6d41\u6c34\u7ebf\u65f6\u5e94\u540c\u65f6\u8ffd\u8e2a\u8f93\u5165\u8bb0\u5f55\u6570\u3001\u8f93\u5165\u4e30\u5ea6\u548c\u8f93\u51fa\u4e30\u5ea6\u3002\u5982\u679c\u8bb0\u5f55\u6570\u51cf\u5c11\u800c\u4e30\u5ea6\u5b88\u6052\uff0c\u901a\u5e38\u7b26\u5408\u65e0\u7b5b\u9009\u53bb\u5197\u4f59\u7684\u9884\u671f\u3002\u5982\u679c\u8f93\u51fa\u4e30\u5ea6\u6070\u597d\u7b49\u4e8e\u4e0a\u4e00\u6b65\u7684\u4ee3\u8868\u5e8f\u5217\u6570\u91cf\uff0c\u5c31\u503c\u5f97\u68c0\u67e5\u8f93\u5165\u4e30\u5ea6\u662f\u5426\u88ab\u4f7f\u7528\u3002<\/p>\n
\u4f4e\u4e30\u5ea6\u8fc7\u6ee4\u7a76\u7adf\u4f9d\u636e\u54ea\u4e2a\u6570\u91cf<\/h2>\n
\u7b2c\u56db\u7ec4\u6062\u590d\u8bfb\u53d6\u8f93\u5165\u4e30\u5ea6\uff0c\u5e76\u8981\u6c42\u53bb\u5197\u4f59\u540e\u7684\u4e30\u5ea6\u81f3\u5c11\u4e3a\u4e09\u3002\u5b9e\u9645 FASTA \u8f93\u51fa\u53ea\u5269\u57fa\u7840\u5e8f\u5217\uff0c\u4e30\u5ea6\u4e3a\u4e8c\u5341\u4e94\u3002\u4e30\u5ea6\u4e3a\u4e8c\u548c\u4e00\u7684\u4e24\u4e2a\u4ee3\u8868\u5e8f\u5217\u4e0d\u518d\u5199\u5165\u8be5\u6587\u4ef6\u3002<\/p>\n
\u7b2c\u4e94\u7ec4\u4fdd\u6301\u76f8\u540c\u95e8\u69db\uff0c\u4f46\u518d\u6b21\u53bb\u6389\u8bfb\u53d6\u8f93\u5165\u4e30\u5ea6\u7684\u9009\u9879\u3002FASTA \u540c\u6837\u53ea\u5269\u4e00\u6761\u4ee3\u8868\u5e8f\u5217\uff0c\u4e30\u5ea6\u5374\u53ea\u6709\u56db\u3002\u4e24\u7ec4\u8f93\u51fa\u7684\u5e8f\u5217\u79cd\u7c7b\u76f8\u540c\uff0c\u8ba1\u6570\u542b\u4e49\u4ecd\u7136\u4e0d\u540c\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n\n\n
\u64cd\u4f5c\u7ec4\u5408<\/th>\n\u6700\u5c0f\u4e30\u5ea6<\/th>\n\u8f93\u51fa\u8bb0\u5f55\u6570<\/th>\n\u4e30\u5ea6\u5408\u8ba1<\/th>\n<\/tr>\n<\/thead>\n
\u6b63\u5411\uff0c\u8bfb\u53d6\u4e30\u5ea6<\/td>\n1<\/td>\n4<\/td>\n28<\/td>\n<\/tr>\n
\u53cc\u94fe\uff0c\u8bfb\u53d6\u4e30\u5ea6<\/td>\n1<\/td>\n3<\/td>\n28<\/td>\n<\/tr>\n
\u53cc\u94fe\uff0c\u6309\u8bb0\u5f55\u8ba1\u6570<\/td>\n1<\/td>\n3<\/td>\n6<\/td>\n<\/tr>\n
\u53cc\u94fe\uff0c\u8bfb\u53d6\u4e30\u5ea6<\/td>\n3<\/td>\n1<\/td>\n25<\/td>\n<\/tr>\n
\u53cc\u94fe\uff0c\u6309\u8bb0\u5f55\u8ba1\u6570<\/td>\n3<\/td>\n1<\/td>\n4<\/td>\n<\/tr>\n
\u65b0\u5165\u53e3\uff0c\u53cc\u94fe\uff0c\u8bfb\u53d6\u4e30\u5ea6<\/td>\n1<\/td>\n3<\/td>\n28<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8fd9\u8bf4\u660e\u53ea\u68c0\u67e5\u6700\u7ec8\u6709\u591a\u5c11\u6761\u4ee3\u8868\u5e8f\u5217\u8fd8\u4e0d\u591f\u3002\u672c\u4f8b\u4e24\u7ec4\u90fd\u662f\u4e00\u6761\uff0c\u770b\u8d77\u6765\u5b8c\u5168\u76f8\u540c\uff0c\u4f46\u540e\u7eed\u4f7f\u7528\u4e30\u5ea6\u505a\u5f52\u4e00\u5316\u6216\u6bd4\u8f83\u65f6\uff0c\u6570\u636e\u542b\u4e49\u5df2\u7ecf\u4e0d\u540c\u3002\u6570\u91cf\u6838\u5bf9\u81f3\u5c11\u5e94\u8be5\u540c\u65f6\u67e5\u770b\u4ee3\u8868\u5e8f\u5217\u6570\u4e0e\u603b\u4e30\u5ea6\u3002<\/p>\n
\u4f4e\u4e30\u5ea6\u4e5f\u4e0d\u80fd\u81ea\u52a8\u7b49\u540c\u4e8e\u9519\u8bef\u3002\u8fd9\u91cc\u7684\u5355\u78b1\u57fa\u53d8\u4f53\u662f\u4eba\u4e3a\u6784\u9020\u7684\u5df2\u77e5\u5dee\u5f02\uff0c\u4e30\u5ea6\u8bbe\u4e3a\u4e8c\u53ea\u662f\u4e3a\u4e86\u6d4b\u8bd5\u95e8\u69db\u3002\u771f\u5b9e\u6837\u672c\u662f\u5426\u5220\u9664\u4f4e\u4e30\u5ea6\u7279\u5f81\uff0c\u9700\u8981\u7ed3\u5408\u9634\u6027\u5bf9\u7167\u3001\u91cd\u590d\u6027\u3001\u6d4b\u5e8f\u8d28\u91cf\u548c\u7814\u7a76\u95ee\u9898\uff0c\u800c\u4e0d\u662f\u4ece\u8fd9\u4e2a\u6559\u5b66\u95e8\u69db\u63a8\u5bfc\u4e00\u4e2a\u901a\u7528\u89c4\u5219\u3002<\/p>\n
UC \u6587\u4ef6\u4e3a\u4ec0\u4e48\u8fd8\u5217\u7740\u88ab\u8fc7\u6ee4\u7684\u5e8f\u5217<\/h2>\n
\u672c\u6b21\u9664\u4e86 FASTA\uff0c\u8fd8\u4fdd\u5b58\u4e86 UC \u5f62\u5f0f\u7684\u6620\u5c04\u7ed3\u679c\u3002\u91cc\u9762\u7684\u79cd\u5b50\u8bb0\u5f55\u3001\u547d\u4e2d\u8bb0\u5f55\u548c\u5206\u7ec4\u6c47\u603b\u53ef\u4ee5\u5e2e\u52a9\u8ffd\u8e2a\u6bcf\u6761\u8f93\u5165\u53bb\u4e86\u54ea\u91cc\u3002\u4f8b\u5982\u53cd\u5411\u4e92\u8865\u8bb0\u5f55\u5728\u53cc\u94fe\u8fd0\u884c\u4e2d\u4ee5\u8d1f\u5411\u547d\u4e2d\u57fa\u7840\u5e8f\u5217\uff0c\u80fd\u591f\u76f4\u63a5\u652f\u6301\u524d\u9762\u7684\u65b9\u5411\u89e3\u91ca\u3002<\/p>\n
\u66f4\u5bb9\u6613\u8bef\u5224\u7684\u662f\u4f4e\u4e30\u5ea6\u7b5b\u9009\u540e\u7684\u8f93\u51fa\u3002\u672c\u6b21\u6700\u5c0f\u4e30\u5ea6\u8bbe\u4e3a\u4e09\u65f6\uff0cFASTA \u53ea\u6709\u4e00\u6761\u4ee3\u8868\u5e8f\u5217\uff0c\u4f46\u5bf9\u5e94 UC \u6587\u4ef6\u4ecd\u5217\u51fa\u4e09\u4e2a\u5206\u7ec4\uff0c\u5305\u62ec\u4e30\u5ea6\u4e3a\u4e8c\u548c\u4e00\u7684\u5bf9\u8c61\u3002\u5b83\u4fdd\u7559\u7684\u662f\u53bb\u5197\u4f59\u6620\u5c04\u5173\u7cfb\uff0c\u4e0d\u80fd\u76f4\u63a5\u5f53\u4f5c\u7b5b\u9009\u540e\u7684\u6700\u7ec8\u7279\u5f81\u6e05\u5355\u3002<\/p>\n
\u56e0\u6b64\uff0c\u5982\u679c\u81ea\u5df1\u7684\u811a\u672c\u4e00\u8fb9\u4f7f\u7528\u7b5b\u9009\u540e\u7684 FASTA\uff0c\u4e00\u8fb9\u6839\u636e\u5b8c\u6574 UC \u6587\u4ef6\u7edf\u8ba1\u7279\u5f81\u6570\uff0c\u5c31\u53ef\u80fd\u5f97\u5230\u76f8\u4e92\u77db\u76fe\u7684\u7ed3\u679c\u3002\u5e94\u5148\u660e\u786e\u6bcf\u4e2a\u8f93\u51fa\u6587\u4ef6\u7684\u7528\u9014\uff0c\u518d\u9009\u62e9\u6700\u7ec8\u4fdd\u7559\u7684\u4ee3\u8868\u5e8f\u5217\u96c6\u5408\u8fdb\u884c\u5173\u8054\u3002<\/p>\n
\u5b9e\u9645\u5ba1\u6838\u65f6\u53ef\u4ee5\u5148\u8bfb\u53d6\u6700\u7ec8 FASTA \u4e2d\u7684\u4ee3\u8868\u5e8f\u5217\uff0c\u5f62\u6210\u4fdd\u7559\u96c6\u5408\uff0c\u518d\u4e0e\u6620\u5c04\u7ed3\u679c\u8fde\u63a5\u3002\u8fd9\u6837\u65e2\u80fd\u8ffd\u8e2a\u539f\u59cb\u6210\u5458\uff0c\u53c8\u4e0d\u4f1a\u628a\u5df2\u7ecf\u4ece\u6700\u7ec8\u5e8f\u5217\u6587\u4ef6\u4e2d\u6392\u9664\u7684\u5206\u7ec4\u91cd\u65b0\u7b97\u56de\u6765\u3002\u672c\u6b21\u5168\u90e8\u6620\u5c04\u884c\u90fd\u4fdd\u5b58\u5728\u4e0b\u8f7d\u5305\u4e2d\uff0c\u8bfb\u8005\u53ef\u4ee5\u9010\u9879\u6838\u5bf9\u3002<\/p>\n
\u65b0\u7248\u63a8\u8350\u5165\u53e3\u4e5f\u5728\u672c\u6b21\u73af\u5883\u4e2d\u8dd1\u8fc7<\/h2>\n
\u6838\u5bf9\u5b98\u65b9\u5728\u7ebf\u6587\u6863\u65f6\uff0c\u5f53\u524d\u6587\u6863\u5df2\u5c06\u4f20\u7edf\u5168\u957f\u53bb\u5197\u4f59\u5165\u53e3\u6807\u4e3a\u5f03\u7528\uff0c\u5e76\u63a8\u8350\u80fd\u591f\u5904\u7406 FASTA \u4e0e FASTQ \u7684\u65b0\u5165\u53e3\u3002\u8fc1\u79fb\u65f6\u8f93\u51fa\u53c2\u6570\u540d\u79f0\u4e5f\u4e0d\u540c\uff0c\u4e0d\u80fd\u53ea\u66ff\u6362\u4e3b\u547d\u4ee4\u540d\u79f0\u3002\u5168\u957f\u53bb\u5197\u4f59\u5b98\u65b9\u8bf4\u660e<\/a><\/p>\n
\u6211\u4eec\u5728\u540c\u4e00\u4e2a 2.30.4 \u73af\u5883\u4e2d\u8865\u8dd1\u4e86\u65b0\u5165\u53e3\uff0c\u4ecd\u4f7f\u7528\u672c\u4f8b\u516d\u6761 FASTA \u8bb0\u5f55\u3001\u53cc\u94fe\u6bd4\u8f83\u548c\u8f93\u5165\u4e30\u5ea6\u3002\u5b9e\u9645\u5f97\u5230\u4e09\u6761\u4ee3\u8868\u5e8f\u5217\u53ca\u4e8c\u5341\u516b\u7684\u4e30\u5ea6\u548c\uff0c\u5206\u522b\u4e3a\u4e8c\u5341\u4e94\u3001\u4e8c\u548c\u4e00\uff0c\u4e0e\u524d\u9762\u7684\u53cc\u94fe\u52a0\u6743\u5bf9\u7167\u4e00\u81f4\u3002<\/p>\n
vsearch --fastx_uniques input.fna \\\r\n  --fastaout both_fastx_sizein.fna \\\r\n  --uc both_fastx_sizein.uc \\\r\n  --strand both \\\r\n  --sizein --sizeout \\\r\n  --minuniquesize 1\r\n<\/code><\/pre>\n
\u8fd9\u4e2a\u7ed3\u679c\u53ea\u9a8c\u8bc1\u4e86\u672c\u6b21\u8f93\u5165\u4e0e\u9009\u9879\u7ec4\u5408\u3002\u771f\u5b9e\u6d41\u7a0b\u8fc1\u79fb\u65f6\uff0c\u4ecd\u5e94\u6838\u5bf9\u957f\u5ea6\u7b5b\u9009\u3001\u8f93\u5165\u7c7b\u578b\u548c\u8d28\u91cf\u503c\u5904\u7406\u7b49\u6761\u4ef6\uff0c\u4e0d\u80fd\u56e0\u4e3a\u8fd9\u4e00\u7ec4\u7ed3\u679c\u76f8\u540c\uff0c\u5c31\u5047\u5b9a\u6240\u6709\u6570\u636e\u90fd\u4f1a\u9010\u5b57\u8282\u4e00\u81f4\u3002\u5c24\u5176\u662f\u5904\u7406\u77ed\u7247\u6bb5\u6216 FASTQ \u65f6\uff0c\u5e94\u6309\u7167\u6240\u7528\u7248\u672c\u7684\u5e2e\u52a9\u4fe1\u606f\u91cd\u65b0\u68c0\u67e5\u3002fastx_uniques \u5b98\u65b9\u8bf4\u660e<\/a><\/p>\n
\u600e\u6837\u628a\u8fd9\u6b21\u68c0\u67e5\u7528\u5230\u81ea\u5df1\u7684\u6d41\u7a0b<\/h2>\n
\u5efa\u8bae\u5148\u4ece\u4e00\u4e2a\u6837\u672c\u62bd\u51fa\u8db3\u591f\u5c0f\u3001\u80fd\u591f\u4eba\u5de5\u590d\u6838\u7684\u8f93\u5165\uff0c\u8bb0\u5f55\u6bcf\u6761\u4ee3\u8868\u5e8f\u5217\u539f\u6765\u627f\u8f7d\u7684\u4e30\u5ea6\u3002\u8fd0\u884c\u540e\u628a\u8f93\u51fa\u4e30\u5ea6\u76f8\u52a0\uff0c\u518d\u6839\u636e\u662f\u5426\u542f\u7528\u8fc7\u6ee4\u5224\u65ad\u603b\u91cf\u53d8\u5316\u662f\u5426\u5408\u7406\u3002\u8fd9\u4e00\u6b65\u80fd\u8f83\u65e9\u53d1\u73b0\u8ba1\u6570\u8bed\u4e49\u9519\u8bef\uff0c\u907f\u514d\u9519\u8bef\u7ee7\u7eed\u4f20\u5230\u591a\u6837\u6027\u6216\u5dee\u5f02\u5206\u6790\u3002<\/p>\n
\u5982\u679c\u8981\u5408\u5e76\u591a\u4e2a\u6837\u672c\uff0c\u8fd8\u9700\u8981\u4fdd\u7559\u6837\u672c\u5f52\u5c5e\u3002\u4e00\u4e2a\u5168\u5c40\u4ee3\u8868\u5e8f\u5217\u7684\u603b\u4e30\u5ea6\u4e0d\u80fd\u4ee3\u66ff\u9010\u6837\u672c\u8ba1\u6570\u8868\u3002\u540c\u4e00\u6761\u5e8f\u5217\u5728\u4e0d\u540c\u6837\u672c\u4e2d\u7684\u5206\u5e03\u5f80\u5f80\u662f\u540e\u7eed\u5206\u6790\u7684\u4e3b\u8981\u4fe1\u606f\uff0c\u5408\u5e76\u6587\u4ef6\u65f6\u4e0d\u5e94\u53ea\u7559\u4e0b\u8de8\u6837\u672c\u7d2f\u52a0\u540e\u7684\u4e00\u4e2a\u6570\u5b57\u3002<\/p>\n
\u6bd4\u8f83\u4e24\u6b21\u53bb\u5197\u4f59\u7ed3\u679c\u65f6\uff0c\u5e94\u4ee5\u5b8c\u6574\u5e8f\u5217\u548c\u5fc5\u8981\u7684\u65b9\u5411\u89c4\u8303\u4e3a\u4f9d\u636e\uff0c\u8c28\u614e\u4f7f\u7528\u4ee3\u8868\u540d\u79f0\u3002\u4ee3\u8868\u540d\u79f0\u53ef\u80fd\u6765\u81ea\u8f93\u5165\u4e2d\u7684\u67d0\u6761\u8bb0\u5f55\uff0c\u5b83\u80fd\u591f\u6807\u8bc6\u672c\u6b21\u8f93\u51fa\uff0c\u4f46\u4e0d\u662f\u8de8\u9879\u76ee\u901a\u7528\u7684\u751f\u7269\u5b66\u7f16\u53f7\u3002\u540d\u79f0\u76f8\u540c\u4e5f\u9700\u8981\u6838\u5bf9\u5b9e\u9645\u5e8f\u5217\uff0c\u540d\u79f0\u4e0d\u540c\u5219\u53ef\u80fd\u53ea\u662f\u8f93\u5165\u987a\u5e8f\u6216\u547d\u540d\u65b9\u5f0f\u4e0d\u540c\u3002<\/p>\n
VSEARCH \u7684\u4f18\u70b9\u662f\u63d0\u4f9b\u660e\u786e\u7684\u547d\u4ee4\u884c\u64cd\u4f5c\u548c\u53ef\u68c0\u67e5\u7684\u6587\u672c\u7ed3\u679c\uff0c\u9002\u5408\u653e\u8fdb\u91cd\u590d\u6267\u884c\u7684\u5e8f\u5217\u5904\u7406\u6d41\u7a0b\u3002\u4f7f\u7528\u4e0a\u7684\u5173\u952e\u9650\u5236\u662f\u6bcf\u4e2a\u5b50\u547d\u4ee4\u8d1f\u8d23\u7684\u4efb\u52a1\u4e0d\u540c\uff0c\u53bb\u5197\u4f59\u7ed3\u679c\u672c\u8eab\u4e0d\u63d0\u4f9b\u7269\u79cd\u8eab\u4efd\uff0c\u4e5f\u4e0d\u8bc1\u660e\u4f4e\u4e30\u5ea6\u53d8\u4f53\u662f\u9519\u8bef\u3002\u5e94\u628a\u5b83\u653e\u5230\u4e00\u4e2a\u8ba1\u6570\u5173\u7cfb\u660e\u786e\u3001\u6b65\u9aa4\u804c\u8d23\u6e05\u695a\u7684\u6d41\u7a0b\u4e2d\u4f7f\u7528\u3002<\/p>\n
\u4e0b\u8f7d\u5305\u5305\u542b\u516d\u6761\u5e8f\u5217\u7684\u751f\u6210\u811a\u672c\u3001\u516d\u7ec4\u5b9e\u9645\u8f93\u51fa\u3001\u5b8c\u6574\u4ee3\u8868\u5e8f\u5217\u3001\u4e30\u5ea6\u53ca UC \u6620\u5c04\u884c\uff0c\u80fd\u591f\u4ece\u8f93\u5165\u91cd\u65b0\u8fd0\u884c\u5e76\u590d\u7b97\u672c\u6587\u8868\u683c\u3002\u4e0b\u8f7d\u5b9e\u8dd1\u811a\u672c\u4e0e\u672c\u6b21\u7ed3\u679c<\/a><\/p>\n
\u5f53\u9879\u76ee\u9700\u8981\u7edf\u4e00\u5904\u7406\u591a\u4e2a\u6d4b\u5e8f\u6279\u6b21\uff0c\u5e76\u4fdd\u7559\u5e8f\u5217\u6620\u5c04\u3001\u4e30\u5ea6\u6838\u5bf9\u548c\u5b8c\u6574\u8fd0\u884c\u8bb0\u5f55\u65f6\uff0c\u53ef\u4e86\u89e3\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u8ba1\u7b97\u670d\u52a1<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u5b9e\u8dd1\u516d\u7ec4 VSEARCH \u5bf9\u7167\uff0c\u89e3\u91ca\u8f93\u5165\u4e30\u5ea6\u3001\u94fe\u65b9\u5411\u4e0e\u4f4e\u4e30\u5ea6\u7b5b\u9009\u5982\u4f55\u6539\u53d8\u4ee3\u8868\u5e8f\u5217\u548c\u8ba1\u6570\uff0c\u5e76\u68c0\u67e5 UC \u4e0e FASTA \u7684\u5dee\u5f02\u3002<\/p>\n","protected":false},"author":1,"featured_media":72773,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[1285,1286,1284],"class_list":["post-72774","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-vsearch","tag-1286","tag-1284"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/vsearch-dereplication-sizein-strand-abundance-practice-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72774","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=72774"}],"version-history":[{"count":2,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72774\/revisions"}],"predecessor-version":[{"id":72787,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72774\/revisions\/72787"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/72773"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=72774"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=72774"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=72774"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}