{"id":72808,"date":"2026-11-15T11:16:09","date_gmt":"2026-11-15T03:16:09","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72808"},"modified":"2026-11-15T11:16:09","modified_gmt":"2026-11-15T03:16:09","slug":"picrust2-copy-number-nsti-function-contribution-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/picrust2-copy-number-nsti-function-contribution-practice\/","title":{"rendered":"PICRUSt2 \u529f\u80fd\u9884\u6d4b\u4e3a\u4f55\u4f1a\u53cd\u8f6c\u6837\u672c\u9ad8\u4f4e\uff1f\u5b9e\u8dd1\u62c6\u89e3\u62f7\u8d1d\u6570\u6821\u6b63\u3001NSTI \u4e0e\u8d21\u732e\u8868"},"content":{"rendered":"
\n

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\u672c\u6587\u5b89\u88c5\u5e76\u8fd0\u884c PICRUSt2 2.6.4\uff0c\u56f4\u7ed5\u4e09\u6761 ASV\u3001\u4e24\u4e2a\u6837\u672c\u548c\u4e24\u4e2a\u8bbe\u8ba1\u529f\u80fd\u5b8c\u6210\u516d\u7ec4\u53c2\u6570\u5bf9\u7167\uff0c\u5e76\u4ece\u5b98\u65b9\u4e09\u5341\u4e03\u6761\u5e8f\u5217\u793a\u4f8b\u8dd1\u901a\u5b8c\u6574\u7684 EC \u4e0e\u901a\u8def\u9884\u6d4b\u3002\u6211\u4eec\u9010\u9879\u590d\u7b97\u6821\u6b63\u540e\u7684\u6743\u91cd\u3001\u529f\u80fd\u603b\u91cf\u548c\u6bcf\u6761 ASV \u7684\u8d21\u732e\uff0c\u540c\u65f6\u68c0\u67e5 NSTI \u95e8\u69db\u5982\u4f55\u6539\u53d8\u6837\u672c\u4e2d\u88ab\u4fdd\u7559\u7684\u90e8\u5206\u3002\u91cd\u70b9\u662f\u8bfb\u61c2\u7ed3\u679c\u4ece\u54ea\u4e9b\u8f93\u5165\u63a8\u5bfc\u800c\u6765\uff0c\u907f\u514d\u628a\u9884\u6d4b\u503c\u5199\u6210\u76f4\u63a5\u6d4b\u91cf\u3002<\/p>\n

\"\u540c\u4e00\u4efd\u8ba1\u6570\uff0c\u529f\u80fd\u9ad8\u4f4e\u4f1a\u53cd\u8f6c\u3002\u516d\u7ec4\u5b9e\u8dd1\u6838\u5bf9\u62f7\u8d1d\u6570\u3001NSTI
\u516d\u7ec4\u5b9e\u8dd1\u6838\u5bf9\u62f7\u8d1d\u6570\u3001NSTI \u4e0e\u8d21\u732e<\/figcaption><\/figure>\n

\u529f\u80fd\u9884\u6d4b\u9700\u8981\u54ea\u4e9b\u4e0d\u540c\u5c42\u6b21\u7684\u8f93\u5165<\/h2>\n

PICRUSt2 \u53ef\u4ee5\u6839\u636e\u6807\u8bb0\u57fa\u56e0\u5e8f\u5217\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u4fe1\u606f\u63a8\u6d4b\u529f\u80fd\u7ec4\u6210\u3002\u5178\u578b\u6d41\u7a0b\u9700\u8981\u5904\u7406\u5e8f\u5217\u653e\u7f6e\u3001\u6027\u72b6\u9884\u6d4b\u4ee5\u53ca\u6837\u672c\u5c42\u9762\u7684\u529f\u80fd\u6c47\u603b\u3002\u8fd9\u51e0\u4e2a\u6b65\u9aa4\u4ea7\u751f\u7684\u6587\u4ef6\u6709\u4e0d\u540c\u542b\u4e49\uff0c\u4e0d\u80fd\u628a\u5176\u4e2d\u4e00\u5f20\u8868\u79f0\u4e3a\u5168\u90e8\u6d4b\u5e8f\u7ed3\u679c\u3002<\/p>\n

\u672c\u6587\u4e3b\u5bf9\u7167\u76f4\u63a5\u4f7f\u7528\u8bbe\u8ba1\u597d\u7684\u6807\u8bb0\u57fa\u56e0\u62f7\u8d1d\u6570\u8868\u548c\u529f\u80fd\u62f7\u8d1d\u6570\u8868\uff0c\u8c03\u7528\u5df2\u5b89\u88c5\u8f6f\u4ef6\u7684\u529f\u80fd\u6c47\u603b\u7a0b\u5e8f\u3002\u8fd9\u6837\u6bcf\u4e00\u4e2a\u8f93\u5165\u6570\u5b57\u90fd\u6709\u660e\u786e\u6765\u6e90\uff0c\u53ef\u4ee5\u72ec\u7acb\u9a8c\u8bc1\u8ba1\u7b97\u8fc7\u7a0b\u3002\u8bbe\u8ba1\u6570\u5b57\u4e0d\u662f\u7531\u8fd9\u4e9b ASV \u7684\u771f\u5b9e\u57fa\u56e0\u7ec4\u6d4b\u5f97\uff0c\u4e5f\u4e0d\u662f\u672c\u6b21\u7528\u7cfb\u7edf\u6811\u63a8\u65ad\u51fa\u6765\u7684\u751f\u7269\u5b66\u4e8b\u5b9e\u3002<\/p>\n

\u6b63\u5f0f\u9879\u76ee\u4e2d\uff0c\u8fd9\u4e24\u7c7b\u9884\u6d4b\u8868\u901a\u5e38\u6765\u81ea\u524d\u9762\u7684\u6027\u72b6\u9884\u6d4b\u6b65\u9aa4\u3002\u628a\u9636\u6bb5\u5206\u5f00\u89e3\u91ca\uff0c\u6709\u52a9\u4e8e\u5b9a\u4f4d\u95ee\u9898\u3002\u82e5\u529f\u80fd\u603b\u91cf\u51fa\u73b0\u610f\u5916\u53d8\u5316\uff0c\u53ef\u4ee5\u5148\u68c0\u67e5\u6c47\u603b\u3001\u6821\u6b63\u548c\u7b5b\u9009\uff0c\u518d\u51b3\u5b9a\u662f\u5426\u9700\u8981\u56de\u5934\u68c0\u67e5\u5e8f\u5217\u653e\u7f6e\u3002\u5b98\u65b9\u529f\u80fd\u6c47\u603b\u6d41\u7a0b<\/a><\/p>\n

\u672c\u6b21\u5b89\u88c5\u4e0e\u590d\u73b0\u65b9\u5f0f<\/h2>\n

\u672c\u6b21\u4f7f\u7528 BioContainers \u63d0\u4f9b\u7684\u56fa\u5b9a\u7248\u672c\u955c\u50cf\uff0c\u5b9e\u9645\u7248\u672c\u547d\u4ee4\u8fd4\u56de 2.6.4\u3002\u590d\u73b0\u5305\u8bb0\u5f55\u955c\u50cf\u6458\u8981\uff0c\u5e76\u63d0\u4f9b\u72ec\u7acb\u9a71\u52a8\uff0c\u91cd\u65b0\u5199\u51fa\u4e09\u4efd\u8f93\u5165\u8868\u540e\u8c03\u7528\u771f\u5b9e\u547d\u4ee4\u884c\u7a0b\u5e8f\u3002<\/p>\n

docker pull quay.io\/biocontainers\/picrust2:2.6.4--pyhdfd78af_0\r\ndocker run --rm \\\r\n  quay.io\/biocontainers\/picrust2:2.6.4--pyhdfd78af_0 \\\r\n  metagenome_pipeline.py --version\r\n<\/code><\/pre>\n
\u8fd9\u91cc\u56fa\u5b9a\u7684\u662f\u672c\u6b21\u4f7f\u7528\u7684\u73af\u5883\uff0c\u5e76\u4e0d\u8981\u6c42\u6240\u6709\u9879\u76ee\u90fd\u91c7\u7528\u76f8\u540c\u5b89\u88c5\u65b9\u5f0f\u3002\u5df2\u6709\u53ef\u8fd0\u884c\u73af\u5883\u65f6\uff0c\u53ef\u4ee5\u5148\u6838\u5bf9\u7248\u672c\u548c\u547d\u4ee4\u5e2e\u52a9\uff0c\u518d\u8fd0\u884c\u4e0b\u8f7d\u5305\u4e2d\u7684\u5bf9\u7167\u3002\u6b63\u5f0f\u4ece\u5e8f\u5217\u5f00\u59cb\u5206\u6790\uff0c\u8fd8\u9700\u8981\u786e\u4fdd\u76f8\u5e94\u53c2\u8003\u6587\u4ef6\u548c\u5916\u90e8\u4f9d\u8d56\u5b8c\u6574\u3002\u5b98\u65b9\u5b89\u88c5\u4e0e\u793a\u4f8b\u8bf4\u660e<\/a><\/p>\n
\u4e09\u6761 ASV \u7684\u8ba1\u6570\u600e\u6837\u5b89\u6392<\/h2>\n
\u4e24\u4e2a\u6837\u672c\u5404\u6709\u4e00\u767e\u4e2a\u8f93\u5165\u8ba1\u6570\u3002\u6837\u672c\u4e00\u4e2d A\u3001B\u3001C \u4e09\u6761 ASV \u7684\u8ba1\u6570\u5206\u522b\u4e3a\u516b\u5341\u3001\u5341\u3001\u5341\uff0c\u6837\u672c\u4e8c\u5206\u522b\u4e3a\u4e8c\u5341\u3001\u516d\u5341\u3001\u4e8c\u5341\u3002<\/p>\n
\u8bbe\u8ba1\u7684\u6807\u8bb0\u57fa\u56e0\u62f7\u8d1d\u6570\u5206\u522b\u4e3a\u4e00\u3001\u4e8c\u3001\u4e94\u3002\u7b2c\u4e00\u4e2a\u529f\u80fd\u5728 A\u3001B\u3001C \u4e2d\u7684\u62f7\u8d1d\u6570\u5206\u522b\u4e3a\u4e00\u3001\u96f6\u3001\u5341\uff0c\u7b2c\u4e8c\u4e2a\u529f\u80fd\u5206\u522b\u4e3a\u96f6\u3001\u4e8c\u3001\u4e94\u3002\u5bf9\u5e94 NSTI \u8bbe\u4e3a 0.05\u30010.2 \u548c 1.5\uff0c\u7528\u4e8e\u89c2\u5bdf\u4e0d\u540c\u95e8\u69db\u7684\u4f5c\u7528\u3002<\/p>\n
\u8fd9\u4e9b\u503c\u523b\u610f\u8ba9\u4e0d\u540c ASV \u5728\u6807\u8bb0\u57fa\u56e0\u6570\u91cf\u4e0e\u529f\u80fd\u6570\u91cf\u4e0a\u4e0d\u4e00\u81f4\u3002\u5373\u4f7f\u4e24\u6761 ASV \u7684\u8bfb\u6bb5\u6570\u76f8\u540c\uff0c\u4e5f\u4e0d\u5fc5\u5728\u6821\u6b63\u540e\u627f\u62c5\u76f8\u540c\u6743\u91cd\uff0c\u66f4\u4e0d\u5fc5\u5bf9\u6bcf\u4e2a\u529f\u80fd\u8d21\u732e\u76f8\u540c\u6570\u91cf\u3002<\/p>\n
\u8f93\u5165\u8868\u7684\u5e8f\u5217\u6807\u8bc6\u5fc5\u987b\u80fd\u591f\u5bf9\u5e94\u8d77\u6765\u3002\u8ba1\u6570\u8868\u3001\u6807\u8bb0\u8868\u548c\u529f\u80fd\u8868\u82e5\u4f7f\u7528\u4e0d\u540c\u547d\u540d\u65b9\u5f0f\uff0c\u53ef\u80fd\u53ea\u5269\u90e8\u5206\u4ea4\u96c6\u3002\u5b9e\u9645\u64cd\u4f5c\u4e2d\u5e94\u5148\u6838\u5bf9\u8fd9\u4e9b\u6807\u8bc6\uff0c\u800c\u4e0d\u662f\u7b49\u5230\u6700\u7ec8\u529f\u80fd\u53d8\u5c11\u65f6\u624d\u6000\u7591\u9884\u6d4b\u7b97\u6cd5\u3002<\/p>\n
\u62f7\u8d1d\u6570\u6821\u6b63\u5148\u6539\u53d8\u6bcf\u6761 ASV \u7684\u6743\u91cd<\/h2>\n
\u9ed8\u8ba4\u6c47\u603b\u4f1a\u5229\u7528\u9884\u6d4b\u7684\u6807\u8bb0\u57fa\u56e0\u62f7\u8d1d\u6570\u8c03\u6574\u8f93\u5165\u8ba1\u6570\u3002\u672c\u4f8b\u6837\u672c\u4e00\u4ece\u516b\u5341\u3001\u5341\u3001\u5341\u53d8\u6210\u516b\u5341\u3001\u4e94\u3001\u4e8c\uff0c\u6837\u672c\u4e8c\u4ece\u4e8c\u5341\u3001\u516d\u5341\u3001\u4e8c\u5341\u53d8\u6210\u4e8c\u5341\u3001\u4e09\u5341\u3001\u56db\u3002<\/p>\n
metagenome_pipeline.py \\\r\n  -i counts.tsv -m marker.tsv -f functions.tsv \\\r\n  --max_nsti 2 --strat_out -o normalized\r\n<\/code><\/pre>\n
\u4f8b\u5982 B \u7684\u6807\u8bb0\u57fa\u56e0\u62f7\u8d1d\u6570\u4e3a\u4e8c\uff0c\u56e0\u6b64\u4e24\u4e2a\u6837\u672c\u4e2d\u5bf9\u5e94\u7684\u8ba1\u6570\u90fd\u9664\u4ee5\u4e8c\u3002C \u7684\u62f7\u8d1d\u6570\u4e3a\u4e94\uff0c\u5176\u6743\u91cd\u76f8\u5e94\u9664\u4ee5\u4e94\u3002\u8f6f\u4ef6\u5b9e\u9645\u8f93\u51fa\u7684\u6821\u6b63\u8868\u4e0e\u8fd9\u4e9b\u8ba1\u7b97\u4e00\u81f4\u3002<\/p>\n
\u6821\u6b63\u540e\u6837\u672c\u4e00\u7684\u6743\u91cd\u548c\u4e3a\u516b\u5341\u4e03\uff0c\u6837\u672c\u4e8c\u4e3a\u4e94\u5341\u56db\u3002\u867d\u7136\u4e24\u4e2a\u6837\u672c\u6700\u521d\u90fd\u6709\u4e00\u767e\u4e2a\u8ba1\u6570\uff0c\u6821\u6b63\u540e\u7684\u603b\u91cf\u5e76\u4e0d\u8981\u6c42\u76f8\u7b49\u3002\u56e0\u6b64\uff0c\u4e0d\u80fd\u7ee7\u7eed\u628a\u539f\u59cb\u7684\u4e00\u767e\u5f53\u4f5c\u6240\u6709\u540e\u7eed\u6bd4\u4f8b\u7684\u56fa\u5b9a\u5206\u6bcd\u3002<\/p>\n
\u8fd9\u4e9b\u6743\u91cd\u4e5f\u4e0d\u5e94\u76f4\u63a5\u5199\u6210\u6837\u672c\u4e2d\u7684\u7edd\u5bf9\u7ec6\u80de\u6570\u91cf\u3002\u5b83\u4eec\u4f9d\u8d56\u6807\u8bb0\u57fa\u56e0\u62f7\u8d1d\u6570\u9884\u6d4b\u4e0e\u5206\u6790\u5047\u8bbe\uff0c\u5c5e\u4e8e\u8be5\u8ba1\u7b97\u6d41\u7a0b\u4e2d\u7684\u6821\u6b63\u91cf\uff0c\u4ecd\u9700\u7ed3\u5408\u5b9e\u9a8c\u8bbe\u8ba1\u548c\u5176\u4ed6\u8bc1\u636e\u89e3\u91ca\u3002<\/p>\n
\u529f\u80fd\u603b\u91cf\u53ef\u4ee5\u600e\u6837\u624b\u7b97<\/h2>\n
\u529f\u80fd\u6c47\u603b\u76f8\u5f53\u4e8e\u628a\u6bcf\u6761 ASV \u7684\u6821\u6b63\u6743\u91cd\u4e58\u4ee5\u76f8\u5e94\u529f\u80fd\u7684\u9884\u6d4b\u62f7\u8d1d\u6570\uff0c\u518d\u5728\u6837\u672c\u5185\u76f8\u52a0\u3002<\/p>\n
\u5bf9\u4e8e\u7b2c\u4e00\u4e2a\u529f\u80fd\uff0c\u6837\u672c\u4e00\u7684\u7ed3\u679c\u662f\u516b\u5341\u4e58\u4e00\uff0c\u52a0\u4e94\u4e58\u96f6\uff0c\u518d\u52a0\u4e8c\u4e58\u5341\uff0c\u5f97\u5230\u4e00\u767e\u3002\u6837\u672c\u4e8c\u5219\u662f\u4e8c\u5341\u4e58\u4e00\uff0c\u52a0\u4e09\u5341\u4e58\u96f6\uff0c\u518d\u52a0\u56db\u4e58\u5341\uff0c\u5f97\u5230\u516d\u5341\u3002<\/p>\n
\u5bf9\u4e8e\u7b2c\u4e8c\u4e2a\u529f\u80fd\uff0c\u6837\u672c\u4e00\u5f97\u5230\u4e8c\u5341\uff0c\u6837\u672c\u4e8c\u5f97\u5230\u516b\u5341\u3002\u7a0b\u5e8f\u8f93\u51fa\u7684\u56db\u4e2a\u6570\u5b57\u4e0e\u624b\u7b97\u4e00\u81f4\uff0c\u8bf4\u660e\u6211\u4eec\u80fd\u591f\u4ece\u8f93\u5165\u8868\u8ffd\u8e2a\u5230\u6700\u7ec8\u529f\u80fd\u8868\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n\n\n
\u6c47\u603b\u6761\u4ef6<\/th>\n\u529f\u80fd<\/th>\n\u6837\u672c\u4e00<\/th>\n\u6837\u672c\u4e8c<\/th>\n<\/tr>\n<\/thead>\n
\u4f7f\u7528\u62f7\u8d1d\u6570\u6821\u6b63<\/td>\n\u529f\u80fd A<\/td>\n100<\/td>\n60<\/td>\n<\/tr>\n
\u4f7f\u7528\u62f7\u8d1d\u6570\u6821\u6b63<\/td>\n\u529f\u80fd B<\/td>\n20<\/td>\n80<\/td>\n<\/tr>\n
\u8df3\u8fc7\u62f7\u8d1d\u6570\u6821\u6b63<\/td>\n\u529f\u80fd A<\/td>\n180<\/td>\n220<\/td>\n<\/tr>\n
\u8df3\u8fc7\u62f7\u8d1d\u6570\u6821\u6b63<\/td>\n\u529f\u80fd B<\/td>\n70<\/td>\n220<\/td>\n<\/tr>\n
\u6821\u6b63\u4e14 NSTI \u4e0d\u9ad8\u4e8e 0.5<\/td>\n\u529f\u80fd A<\/td>\n80<\/td>\n20<\/td>\n<\/tr>\n
\u6821\u6b63\u4e14 NSTI \u4e0d\u9ad8\u4e8e 0.5<\/td>\n\u529f\u80fd B<\/td>\n10<\/td>\n60<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u5efa\u8bae\u5728\u771f\u5b9e\u7ed3\u679c\u4e2d\u6311\u9009\u5c11\u91cf\u529f\u80fd\uff0c\u624b\u52a8\u590d\u7b97\u51e0\u4e2a\u6837\u672c\u3002\u8fd9\u6837\u53ef\u4ee5\u8f83\u65e9\u53d1\u73b0\u8868\u683c\u65b9\u5411\u5f04\u53cd\u3001\u529f\u80fd\u5217\u8bfb\u9519\u6216\u8ba1\u6570\u5355\u4f4d\u6539\u53d8\u7b49\u95ee\u9898\uff0c\u6bd4\u53ea\u67e5\u770b\u6700\u540e\u7684\u6392\u5e8f\u56fe\u66f4\u5bb9\u6613\u5224\u65ad\u6d41\u7a0b\u662f\u5426\u6309\u9884\u671f\u6267\u884c\u3002<\/p>\n
\u8df3\u8fc7\u6821\u6b63\u540e\uff0c\u6837\u672c\u987a\u5e8f\u771f\u7684\u53d1\u751f\u4e86\u53cd\u8f6c<\/h2>\n
\u7b2c\u4e8c\u7ec4\u5b9e\u9645\u542f\u7528\u8df3\u8fc7\u6821\u6b63\u9009\u9879\uff0c\u5e76\u6309\u7a0b\u5e8f\u8981\u6c42\u4e0d\u518d\u4f20\u5165\u6807\u8bb0\u57fa\u56e0\u8868\u3002\u5176\u4ed6\u529f\u80fd\u8f93\u5165\u548c\u6837\u672c\u8ba1\u6570\u4fdd\u6301\u4e0d\u53d8\u3002<\/p>\n
metagenome_pipeline.py \\\r\n  -i counts.tsv -f functions.tsv \\\r\n  --skip_norm --max_nsti 2 \\\r\n  --strat_out -o skip_normalization\r\n<\/code><\/pre>\n
\u7b2c\u4e00\u4e2a\u529f\u80fd\u7684\u6837\u672c\u4e00\u7ed3\u679c\u53d8\u6210\u4e00\u767e\u516b\u5341\uff0c\u6837\u672c\u4e8c\u53d8\u6210\u4e8c\u767e\u4e8c\u5341\u3002\u4e0e\u9ed8\u8ba4\u6821\u6b63\u540e\u7684\u7b2c\u4e00\u7ec4\u76f8\u6bd4\uff0c\u9ad8\u4f4e\u987a\u5e8f\u786e\u5b9e\u53cd\u8f6c\u4e86\u3002<\/p>\n
\u539f\u56e0\u53ef\u4ee5\u76f4\u63a5\u8ffd\u5230 C\u3002\u5b83\u5728\u6837\u672c\u4e8c\u6709\u4e8c\u5341\u4e2a\u539f\u59cb\u8ba1\u6570\uff0c\u5e76\u88ab\u8bbe\u8ba1\u4e3a\u5177\u6709\u5341\u4e2a\u8be5\u529f\u80fd\u62f7\u8d1d\u3002\u8df3\u8fc7\u6821\u6b63\u65f6\uff0c\u8fd9\u90e8\u5206\u8d21\u732e\u8fbe\u5230\u4e8c\u767e\uff0c\u800c\u9ed8\u8ba4\u6821\u6b63\u540e\u53ea\u6709\u56db\u4e58\u5341\uff0c\u4e5f\u5c31\u662f\u56db\u5341\u3002<\/p>\n
\u8fd9\u4e2a\u5bf9\u7167\u8bf4\u660e\uff0c\u6539\u53d8\u5f52\u4e00\u5316\u65b9\u6cd5\u53ef\u80fd\u6539\u53d8\u540e\u7eed\u6bd4\u8f83\u65b9\u5411\u3002\u4f46\u672c\u4f8b\u53ea\u6709\u4e24\u4e2a\u8bbe\u8ba1\u6837\u672c\uff0c\u6ca1\u6709\u751f\u7269\u5b66\u91cd\u590d\uff0c\u56e0\u6b64\u4e0d\u8fdb\u884c\u663e\u8457\u6027\u5224\u65ad\uff0c\u4e5f\u4e0d\u628a\u67d0\u4e00\u79cd\u8f93\u51fa\u987a\u5e8f\u79f0\u4e3a\u771f\u5b9e\u6548\u5e94\u3002\u6b63\u5f0f\u9879\u76ee\u5e94\u9884\u5148\u660e\u786e\u6821\u6b63\u65b9\u6cd5\uff0c\u5e76\u5728\u89e3\u91ca\u65f6\u8bf4\u660e\u8f93\u5165\u4e0e\u5047\u8bbe\u3002<\/p>\n
NSTI \u95e8\u69db\u6539\u53d8\u7684\u662f\u54ea\u4e9b\u8f93\u5165\u8fd8\u80fd\u53c2\u4e0e<\/h2>\n
\u672c\u4f8b\u8fdb\u4e00\u6b65\u628a\u6700\u5927 NSTI \u8bbe\u4e3a 0.5\u3002C \u7684\u8bbe\u8ba1 NSTI \u4e3a 1.5\uff0c\u56e0\u6b64\u88ab\u6392\u9664\uff0cA \u4e0e B \u7ee7\u7eed\u53c2\u4e0e\u3002\u5b9e\u9645\u4e24\u4e2a\u529f\u80fd\u7684\u7ed3\u679c\u5206\u522b\u53d8\u4e3a\u6837\u672c\u4e00\u7684\u516b\u5341\u548c\u5341\uff0c\u4ee5\u53ca\u6837\u672c\u4e8c\u7684\u4e8c\u5341\u548c\u516d\u5341\u3002<\/p>\n
\u8fd9\u4e2a\u53d8\u5316\u540c\u65f6\u5f71\u54cd\u6837\u672c\u4fdd\u7559\u8303\u56f4\u3002\u6309\u539f\u59cb\u8f93\u5165\u8ba1\u6570\u6838\u5bf9\uff0c\u6837\u672c\u4e00\u4fdd\u7559\u4e5d\u5341\uff0c\u6837\u672c\u4e8c\u4fdd\u7559\u516b\u5341\u3002\u867d\u7136\u90fd\u5220\u9664\u540c\u4e00\u6761 ASV\uff0c\u4e24\u4e2a\u6837\u672c\u635f\u5931\u7684\u6bd4\u4f8b\u5e76\u4e0d\u76f8\u540c\u3002<\/p>\n
\u5982\u679c\u8fdb\u4e00\u6b65\u5c06\u95e8\u69db\u8bbe\u4e3a 0.1\uff0c\u5c31\u53ea\u7559\u4e0b A\u3002\u4e24\u4e2a\u6837\u672c\u5206\u522b\u53ea\u4fdd\u7559\u539f\u59cb\u8ba1\u6570\u7684\u516b\u6210\u548c\u4e24\u6210\uff0c\u7b2c\u4e00\u4e2a\u529f\u80fd\u8f93\u51fa\u516b\u5341\u4e0e\u4e8c\u5341\uff0c\u7b2c\u4e8c\u4e2a\u529f\u80fd\u4e0d\u518d\u51fa\u73b0\u5728\u6700\u7ec8\u529f\u80fd\u8868\u4e2d\u3002<\/p>\n
\u56e0\u6b64\uff0c\u62a5\u544a\u8fc7\u6ee4\u7ed3\u679c\u65f6\uff0c\u9664\u4e86\u7edf\u8ba1\u5220\u9664\u4e86\u591a\u5c11\u6761 ASV\uff0c\u8fd8\u5e94\u6838\u5bf9\u6bcf\u4e2a\u6837\u672c\u4e22\u5931\u4e86\u591a\u5c11\u4e30\u5ea6\u3002\u76f8\u540c\u7684\u7279\u5f81\u6570\u91cf\u53d8\u5316\uff0c\u53ef\u80fd\u5bf9\u4e0d\u540c\u6837\u672c\u4ea7\u751f\u5f88\u4e0d\u4e00\u6837\u7684\u5f71\u54cd\u3002<\/p>\n
NSTI \u4e0e\u53c2\u8003\u4ee3\u8868\u6027\u6709\u5173\uff0c\u4f46\u4e0d\u80fd\u88ab\u5f53\u4f5c\u6bcf\u4e2a\u529f\u80fd\u9884\u6d4b\u90fd\u5fc5\u7136\u51c6\u786e\u7684\u8bc1\u660e\u3002\u53c2\u8003\u8986\u76d6\u3001\u83cc\u682a\u95f4\u57fa\u56e0\u5dee\u5f02\u4ee5\u53ca\u6807\u8bb0\u57fa\u56e0\u5206\u8fa8\u80fd\u529b\u4ecd\u4f1a\u5f71\u54cd\u89e3\u91ca\u3002\u5b98\u65b9\u5c40\u9650\u8bf4\u660e<\/a><\/p>\n
\u52a0\u6743 NSTI \u4e3a\u4ec0\u4e48\u4e5f\u8981\u6838\u5bf9\u6743\u91cd<\/h2>\n
\u9ed8\u8ba4\u6821\u6b63\u7ec4\u5b9e\u9645\u8f93\u51fa\u7684\u6837\u672c\u52a0\u6743 NSTI \u5206\u522b\u7ea6\u4e3a 0.09195 \u548c 0.24074\u3002\u4ee5\u6837\u672c\u4e00\u4e3a\u4f8b\uff0c\u5c06\u516b\u5341\u4e58 0.05\u3001\u4e94\u4e58 0.2\u3001\u4e8c\u4e58 1.5 \u76f8\u52a0\uff0c\u5f97\u5230\u516b\uff0c\u518d\u9664\u4ee5\u6821\u6b63\u6743\u91cd\u548c\u516b\u5341\u4e03\uff0c\u5c31\u80fd\u590d\u7b97\u8be5\u503c\u3002<\/p>\n
\u8fd9\u8bf4\u660e\u672c\u6b21\u9ed8\u8ba4\u6c47\u603b\u4e2d\u7684\u52a0\u6743\u4f7f\u7528\u4e86\u6821\u6b63\u540e\u7684\u6570\u91cf\u3002\u5982\u679c\u76f4\u63a5\u62ff\u539f\u59cb\u8ba1\u6570\u8ba1\u7b97\uff0c\u4f1a\u5f97\u5230\u53e6\u4e00\u7ec4\u6570\u503c\u3002\u8df3\u8fc7\u6821\u6b63\u7684\u5b9e\u9645\u8fd0\u884c\u4e2d\uff0c\u4e24\u4e2a\u6837\u672c\u7684\u52a0\u6743 NSTI \u6b63\u597d\u4e3a 0.21 \u548c 0.43\u3002<\/p>\n
\u95e8\u69db\u964d\u4e3a 0.1 \u540e\uff0c\u4e24\u4efd\u6837\u672c\u7684\u52a0\u6743 NSTI \u90fd\u53d8\u6210 0.05\uff0c\u56e0\u4e3a\u53ea\u5269\u4e0b A\u3002\u6570\u5b57\u770b\u8d77\u6765\u66f4\u4f4e\uff0c\u4f46\u6837\u672c\u4e8c\u53ea\u4fdd\u7559\u4e86\u539f\u59cb\u8ba1\u6570\u7684\u4e24\u6210\u3002\u8bc4\u4ef7\u7ed3\u679c\u65f6\u5e94\u628a\u8fd9\u4e2a\u6570\u4e0e\u6837\u672c\u4fdd\u7559\u6bd4\u4f8b\u4e00\u8d77\u67e5\u770b\u3002<\/p>\n
\u4ec5\u9009\u62e9\u4e00\u4e2a\u770b\u8d77\u6765\u66f4\u597d\u7684\u5e73\u5747\u6307\u6807\uff0c\u5bb9\u6613\u5ffd\u7565\u88ab\u7b5b\u6389\u7684\u6570\u636e\u3002\u66f4\u5b8c\u6574\u7684\u8bb0\u5f55\u5e94\u5305\u542b\u6bcf\u6761 ASV \u7684 NSTI\u3001\u6821\u6b63\u65b9\u6cd5\u3001\u95e8\u69db\u548c\u6837\u672c\u4fdd\u7559\u91cf\uff0c\u8ba9\u8bfb\u8005\u80fd\u591f\u5224\u65ad\u6700\u7ec8\u9884\u6d4b\u8986\u76d6\u4e86\u8f93\u5165\u4e2d\u7684\u54ea\u4e00\u90e8\u5206\u3002<\/p>\n
\u8d21\u732e\u8868\u53ef\u4ee5\u89e3\u91ca\u8c01\u5728\u9a71\u52a8\u67d0\u4e2a\u529f\u80fd<\/h2>\n
\u5f00\u542f\u5206\u5c42\u8f93\u51fa\u540e\uff0c\u7a0b\u5e8f\u4f1a\u628a\u6837\u672c\u529f\u80fd\u91cf\u62c6\u5230\u5177\u4f53 ASV\u3002\u9ed8\u8ba4\u6821\u6b63\u7ec4\u4e2d\uff0c\u6837\u672c\u4e00\u7b2c\u4e00\u4e2a\u529f\u80fd\u7531 A \u8d21\u732e\u516b\u5341\u3001C \u8d21\u732e\u4e8c\u5341\uff0c\u4e8c\u8005\u5206\u522b\u5360\u8be5\u529f\u80fd\u603b\u91cf\u7684\u516b\u6210\u548c\u4e24\u6210\u3002<\/p>\n
\u6837\u672c\u4e8c\u5219\u7531 A \u8d21\u732e\u4e8c\u5341\u3001C \u8d21\u732e\u56db\u5341\u3002C \u5728\u6821\u6b63\u540e\u7684\u6837\u672c\u6743\u91cd\u4e2d\u53ea\u5360\u7ea6\u767e\u5206\u4e4b\u4e03\u70b9\u56db\uff0c\u5374\u8d21\u732e\u4e86\u8be5\u529f\u80fd\u603b\u91cf\u7684\u4e09\u5206\u4e4b\u4e8c\u3002\u539f\u56e0\u662f\u5b83\u5728\u8bbe\u8ba1\u8868\u4e2d\u5177\u6709\u8f83\u591a\u7684\u8be5\u529f\u80fd\u62f7\u8d1d\u3002<\/p>\n
\u56e0\u6b64\uff0cASV \u5728\u6837\u672c\u4e2d\u7684\u6bd4\u4f8b\u4e0e\u5b83\u5bf9\u67d0\u4e2a\u529f\u80fd\u7684\u8d21\u732e\u6bd4\u4f8b\u662f\u4e24\u79cd\u4e0d\u540c\u7684\u6570\u3002\u524d\u8005\u4ee5\u6837\u672c\u4e2d\u7684 ASV \u6743\u91cd\u4e3a\u5206\u6bcd\uff0c\u540e\u8005\u4ee5\u7279\u5b9a\u529f\u80fd\u7684\u603b\u91cf\u4e3a\u5206\u6bcd\uff0c\u4e0d\u80fd\u76f4\u63a5\u4e92\u6362\u3002<\/p>\n
\u9605\u8bfb\u8d21\u732e\u8868\u65f6\uff0c\u5e94\u660e\u786e\u81ea\u5df1\u53d6\u7684\u662f\u54ea\u4e00\u5217\u3002\u672c\u6b21\u7528\u4e8e\u590d\u7b97\u529f\u80fd\u8d21\u732e\u7684\u662f\u6bcf\u6761 ASV \u5bf9\u8be5\u529f\u80fd\u7684\u8d21\u732e\u91cf\uff0c\u4ee5\u53ca\u5728\u8be5\u529f\u80fd\u5185\u90e8\u5f52\u4e00\u5316\u7684\u8d21\u732e\u6bd4\u4f8b\u3002\u540d\u79f0\u4e2d\u542b\u6709\u76f8\u5bf9\u91cf\u7684\u5176\u4ed6\u5217\uff0c\u4e5f\u9700\u8981\u7ed3\u5408\u5206\u6bcd\u89e3\u91ca\uff0c\u4e0d\u80fd\u4e00\u5f8b\u6309\u540c\u4e00\u79cd\u767e\u5206\u6bd4\u5904\u7406\u3002<\/p>\n
RARE \u6807\u7b7e\u6ca1\u6709\u628a\u529f\u80fd\u603b\u91cf\u5220\u6389<\/h2>\n
\u53e6\u4e00\u7ec4\u5c06\u6700\u5c0f\u8bfb\u6570\u95e8\u69db\u8bbe\u4e3a\u4e09\u5341\u4e94\uff0c\u5e76\u4fdd\u7559\u5206\u5c42\u8f93\u51fa\u3002\u5b9e\u9645\u8d21\u732e\u8868\u4e2d C \u88ab\u5408\u5e76\u5230 RARE \u7c7b\u522b\uff0c\u4f46\u4e24\u4e2a\u529f\u80fd\u7684\u6837\u672c\u603b\u91cf\u4e0e\u9ed8\u8ba4\u7ec4\u5b8c\u5168\u76f8\u540c\u3002<\/p>\n
\u8fd9\u8bf4\u660e\u672c\u6b21\u53c2\u6570\u6539\u53d8\u7684\u662f\u8d21\u732e\u8868\u4e2d\u7684\u5f52\u7c7b\u65b9\u5f0f\uff0c\u6ca1\u6709\u628a\u5bf9\u5e94\u529f\u80fd\u8d21\u732e\u76f4\u63a5\u4ece\u603b\u91cf\u4e2d\u53bb\u6389\u3002\u82e5\u53ea\u641c\u7d22\u539f\u6765\u7684 ASV \u540d\u79f0\uff0c\u53ef\u80fd\u8bef\u4ee5\u4e3a\u5b83\u6d88\u5931\u4e86\uff0c\u4f46\u5728\u6c47\u603b\u7c7b\u522b\u91cc\u4ecd\u80fd\u627e\u5230\u76f8\u5e94\u8d21\u732e\u3002<\/p>\n
\u6574\u7406\u62a5\u544a\u65f6\uff0c\u5e94\u628a\u7279\u5f81\u7b5b\u9664\u4e0e\u8d21\u732e\u8005\u5408\u5e76\u533a\u5206\u5f00\u3002\u524d\u9762\u7684 NSTI \u7b5b\u9009\u6539\u53d8\u4e86\u53c2\u4e0e\u6c47\u603b\u7684\u8f93\u5165\uff0c\u5f53\u524d\u8fd9\u7ec4\u7a00\u6709\u7c7b\u522b\u5904\u7406\u4e3b\u8981\u6539\u53d8\u8d21\u732e\u7684\u5c55\u793a\u7c92\u5ea6\uff0c\u4e8c\u8005\u4e0d\u5e94\u4f7f\u7528\u540c\u4e00\u4e2a\u201c\u5220\u9664\u4f4e\u4e30\u5ea6\u201d\u6807\u7b7e\u6982\u62ec\u3002<\/p>\n
\u4ece\u5b98\u65b9\u5e8f\u5217\u793a\u4f8b\u5f00\u59cb\uff0c\u73af\u5883\u95ee\u9898\u53d1\u751f\u5728\u54ea\u4e00\u6b65<\/h2>\n
\u524d\u9762\u7684\u516d\u7ec4\u5bf9\u7167\u9a8c\u8bc1\u529f\u80fd\u6c47\u603b\u3002\u4e3a\u4e86\u68c0\u67e5\u4ece\u5e8f\u5217\u5f00\u59cb\u7684\u5b9e\u9645\u8def\u5f84\uff0c\u672c\u6b21\u8fd8\u4e0b\u8f7d\u5b98\u65b9\u5b89\u88c5\u9875\u9762\u63d0\u4f9b\u7684\u793a\u4f8b\u5f52\u6863\uff0c\u6838\u5bf9\u6587\u4ef6\u6821\u9a8c\u503c\uff0c\u5e76\u4f7f\u7528\u5176\u4e2d\u7684\u4ee3\u8868\u5e8f\u5217\u548c BIOM \u8ba1\u6570\u8868\u8fd0\u884c\u5b8c\u6574\u6d41\u7a0b\u3002\u8fd9\u6837\u80fd\u591f\u628a\u6211\u4eec\u4eba\u4e3a\u8bbe\u7f6e\u7684\u6570\u503c\u5bf9\u7167\u4e0e\u771f\u5b9e\u53c2\u8003\u6811\u63a8\u65ad\u533a\u5206\u5f00\u6765\u3002<\/p>\n
\u793a\u4f8b\u53ea\u6709\u4e09\u5341\u4e03\u6761\u5e8f\u5217\uff0c\u4f46\u5e8f\u5217\u653e\u7f6e\u9700\u8981\u4f7f\u7528\u53c2\u8003\u6570\u636e\u3002\u7b2c\u4e00\u6b21\u4ee5\u5341\u516d GiB \u5bb9\u5668\u5185\u5b58\u4e0a\u9650\u8fd0\u884c\u663e\u5f0f EC \u8868\u6d41\u7a0b\u65f6\uff0c\u7ec6\u83cc EPA-NG \u9636\u6bb5\u5931\u8d25\uff0c\u5bb9\u5668\u72b6\u6001\u660e\u786e\u8bb0\u5f55\u5185\u5b58\u9650\u5236\u89e6\u53d1\u7ec8\u6b62\u3002\u968f\u540e\u628a\u4e0a\u9650\u6539\u4e3a\u4e8c\u5341\u56db GiB\u3001\u8fdb\u7a0b\u6570\u6539\u4e3a\u4e00\uff0c\u7ec6\u83cc\u548c\u53e4\u83cc\u653e\u7f6e\u9636\u6bb5\u90fd\u901a\u8fc7\u3002\u8fd9\u4e2a\u7ecf\u9a8c\u8bf4\u660e\uff0c\u4e0d\u80fd\u4ec5\u7528\u8f93\u5165\u5e8f\u5217\u6570\u91cf\u63a8\u65ad\u53c2\u8003\u8ba1\u7b97\u9636\u6bb5\u7684\u8d44\u6e90\u9700\u6c42\uff0c\u4e5f\u4e0d\u80fd\u628a\u6240\u6709\u975e\u96f6\u9000\u51fa\u90fd\u5f52\u4e3a\u540c\u4e00\u79cd\u6545\u969c\u3002<\/p>\n
\u672c\u6b21\u8fd8\u68c0\u67e5\u4e86\u56fa\u5b9a\u7248\u672c\u7684\u6d41\u7a0b\u6e90\u7801\u3002\u9ed8\u8ba4\u53cc\u57df\u6d41\u7a0b\u5728\u51c6\u5907\u9636\u6bb5\u4f1a\u68c0\u67e5\u591a\u5957\u9ed8\u8ba4\u6027\u72b6\u8868\uff0c\u4ec5\u9009\u62e9 EC \u8f93\u51fa\u5e76\u4e0d\u7b49\u4e8e\u51c6\u5907\u65f6\u5b8c\u5168\u4e0d\u8bfb\u53d6\u5176\u4ed6\u8868\u3002\u4e3a\u660e\u786e\u5b9e\u9a8c\u8303\u56f4\uff0c\u672c\u6b21\u5c06\u955c\u50cf\u81ea\u5e26\u7684\u7ec6\u83cc\u4e0e\u53e4\u83cc EC \u8868\u5206\u522b\u901a\u8fc7\u4e24\u9879\u81ea\u5b9a\u4e49\u6027\u72b6\u8868\u53c2\u6570\u4f20\u5165\uff0c\u540c\u65f6\u5c06\u53cd\u5e94\u7c7b\u578b\u8bbe\u4e3a ec.txt<\/code>\u3002\u4f7f\u7528\u7684\u4ecd\u662f\u5b89\u88c5\u5305\u81ea\u5e26\u53c2\u8003\uff0c\u6ca1\u6709\u624b\u5de5\u6539\u5199\u9884\u6d4b\u503c\u3002<\/p>\n
\u8fd9\u79cd\u663e\u5f0f\u4f20\u8868\u65b9\u5f0f\u4f1a\u5f71\u54cd\u8f93\u51fa\u540d\u79f0\u3002\u672c\u6b21\u529f\u80fd\u6c47\u603b\u76ee\u5f55\u53eb ec.txt_metagenome_out<\/code>\uff0c\u5982\u679c\u6cbf\u7528\u53e6\u4e00\u5957\u6559\u7a0b\u4e2d\u7684\u76ee\u5f55\u540d\u79f0\u5bfb\u627e\u7ed3\u679c\uff0c\u53ef\u80fd\u8bef\u5224\u6ca1\u6709\u751f\u6210\u6587\u4ef6\u3002\u5b9e\u9645\u5904\u7406\u5e94\u5148\u68c0\u67e5\u8fd0\u884c\u547d\u4ee4\u3001\u65e5\u5fd7\u548c\u76ee\u5f55\u5217\u8868\uff0c\u518d\u628a\u5177\u4f53\u6587\u4ef6\u8def\u5f84\u4ea4\u7ed9\u4e0b\u6e38\u811a\u672c\u3002<\/p>\n
\u5e8f\u5217\u901a\u8fc7\u4e24\u5957\u53c2\u8003\u653e\u7f6e\u540e\uff0c\u8f6f\u4ef6\u6bd4\u8f83\u5e76\u9009\u62e9\u5bf9\u5e94\u57df\u3002\u672c\u6b21\u4e09\u5341\u4e94\u6761\u88ab\u4fdd\u7559\u5230\u7ec6\u83cc\u53c2\u8003\u5206\u652f\uff0c\u4e24\u6761\u88ab\u4fdd\u7559\u5230\u53e4\u83cc\u53c2\u8003\u5206\u652f\u3002\u4e0d\u80fd\u56e0\u4e3a\u4e0b\u8f7d\u6587\u4ef6\u540d\u4e2d\u51fa\u73b0\u53e4\u83cc\u76f8\u5173\u7f29\u5199\uff0c\u5c31\u628a\u6574\u4efd\u8f93\u5165\u5f53\u6210\u5168\u90e8\u6765\u81ea\u53e4\u83cc\u3002\u8fd9\u7c7b\u6837\u672c\u8bf4\u660e\u5fc5\u987b\u4f9d\u636e\u6587\u4ef6\u5185\u5bb9\u548c\u5b9e\u9645\u8f93\u51fa\uff0c\u800c\u4e0d\u80fd\u53ea\u770b\u76ee\u5f55\u540d\u79f0\u3002<\/p>\n
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\u8fd9\u6b21\u5b98\u65b9\u793a\u4f8b\u7684\u8f93\u5165\u8868\u5305\u542b\u4e8c\u5341\u56db\u4e2a\u6837\u672c\uff0c\u603b\u8ba1\u5341\u4e07\u516b\u5343\u4e03\u767e\u4e00\u5341\u516b\u4e2a\u8ba1\u6570\u3002\u5b8c\u6574\u6d41\u7a0b\u6700\u7ec8\u6b63\u5e38\u9000\u51fa\uff0c\u4e09\u5341\u4e03\u6761\u5e8f\u5217\u5168\u90e8\u8fdb\u5165\u529f\u80fd\u6c47\u603b\uff0c\u8f93\u51fa\u4e00\u5343\u4e09\u767e\u4e09\u5341\u4e00\u4e2a EC \u529f\u80fd\u6761\u76ee\u548c\u4e09\u767e\u4e00\u5341\u4e8c\u4e2a\u901a\u8def\u6761\u76ee\uff0c\u4e24\u4e2a\u7ed3\u679c\u8868\u5747\u4fdd\u7559\u4e8c\u5341\u56db\u4e2a\u6837\u672c\u3002<\/p>\n
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\u6240\u6709 ASV \u90fd\u88ab\u7b5b\u6389\u65f6\uff0c\u5e94\u8be5\u600e\u6837\u8bfb\u7ed3\u679c<\/h2>\n
\u6700\u540e\u4e00\u7ec4\u5c06 NSTI \u95e8\u69db\u964d\u5230 0.01\uff0c\u4f4e\u4e8e\u4e09\u6761\u8bbe\u8ba1 ASV \u7684\u6570\u503c\u3002\u7a0b\u5e8f\u5b9e\u9645\u4ee5\u975e\u96f6\u72b6\u6001\u9000\u51fa\uff0c\u660e\u786e\u63d0\u793a\u6240\u6709 ASV \u90fd\u88ab\u8fc7\u6ee4\uff0c\u6ca1\u6709\u751f\u6210\u53ef\u7528\u529f\u80fd\u8868\u3002<\/p>\n
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PICRUSt2 \u7684\u4ef7\u503c\u5728\u4e8e\u5229\u7528\u5df2\u6709\u6807\u8bb0\u57fa\u56e0\u6570\u636e\u5f62\u6210\u53ef\u4ee5\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u7684\u529f\u80fd\u5047\u8bbe\u3002\u5b83\u7684\u5c40\u9650\u662f\u8fd9\u4e9b\u7ed3\u679c\u4f9d\u8d56\u53c2\u8003\u4e0e\u9884\u6d4b\u6b65\u9aa4\uff0c\u4e0d\u80fd\u66ff\u4ee3\u5bf9\u6837\u672c\u529f\u80fd\u57fa\u56e0\u7684\u76f4\u63a5\u89c2\u6d4b\uff0c\u66f4\u4e0d\u80fd\u4ec5\u51ed\u9884\u6d4b\u4e30\u5ea6\u63a8\u65ad\u529f\u80fd\u5df2\u7ecf\u8868\u8fbe\u6216\u53d1\u751f\u4e86\u7279\u5b9a\u751f\u5316\u53cd\u5e94\u3002<\/p>\n
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