{"id":72810,"date":"2026-11-15T11:16:11","date_gmt":"2026-11-15T03:16:11","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72810"},"modified":"2026-11-15T11:16:11","modified_gmt":"2026-11-15T03:16:11","slug":"sourmash-jaccard-containment-scaled-direction-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/sourmash-jaccard-containment-scaled-direction-practice\/","title":{"rendered":"sourmash \u76f8\u4f3c\u5ea6\u53ea\u6709 20.78%\uff0c\u4e3a\u4ec0\u4e48\u53c8\u663e\u793a\u5b8c\u5168\u5305\u542b\uff1f\u7528\u771f\u5b9e\u53c2\u8003\u5e8f\u5217\u5b9e\u8dd1\u8349\u56fe\u6bd4\u8f83"},"content":{"rendered":"
\n

\u7528 sourmash \u641c\u7d22\u5e8f\u5217\u65f6\uff0c\u540c\u4e00\u5bf9\u8f93\u5165\u53ef\u80fd\u5f97\u5230\u4e00\u4e2a\u4e0d\u9ad8\u7684\u76f8\u4f3c\u5ea6\uff0c\u4e5f\u53ef\u80fd\u5f97\u5230\u767e\u5206\u4e4b\u767e\u7684\u5305\u542b\u5ea6\u3002\u8fd9\u4e24\u4e2a\u7ed3\u679c\u5e76\u4e0d\u51b2\u7a81\u3002\u524d\u8005\u5173\u5fc3\u4e24\u4efd\u5e8f\u5217\u5185\u5bb9\u603b\u4f53\u6709\u591a\u76f8\u50cf\uff0c\u540e\u8005\u5173\u5fc3\u67d0\u4e00\u4efd\u67e5\u8be2\u5185\u5bb9\u6709\u591a\u5c11\u80fd\u5728\u53e6\u4e00\u4efd\u6570\u636e\u4e2d\u627e\u5230\u3002<\/p>\n

\u672c\u6587\u5b89\u88c5\u5e76\u8fd0\u884c sourmash 4.8.14\uff0c\u4f7f\u7528\u516c\u5f00\u53c2\u8003\u57fa\u56e0\u7ec4\u53ca\u5176\u622a\u53d6\u7247\u6bb5\uff0c\u5b9e\u9645\u751f\u6210\u8349\u56fe\u3001\u641c\u7d22\u548c\u5206\u89e3\u6df7\u5408\u8f93\u5165\u3002\u901a\u8fc7\u53ef\u4ee5\u72ec\u7acb\u590d\u7b97\u7684\u96c6\u5408\u6570\u91cf\uff0c\u89e3\u91ca\u6bd4\u8f83\u65b9\u5411\u3001\u91c7\u6837\u5bc6\u5ea6\u3001\u91cd\u590d\u5e8f\u5217\u548c\u53c2\u6570\u517c\u5bb9\u6027\u3002\u8fd9\u6837\u770b\u5230\u4e00\u4e2a\u767e\u5206\u6bd4\u65f6\uff0c\u5c31\u80fd\u5148\u786e\u8ba4\u5b83\u7684\u5206\u6bcd\uff0c\u800c\u4e0d\u662f\u76f4\u63a5\u628a\u5b83\u5f53\u6210\u7269\u79cd\u4e00\u81f4\u7387\u3002<\/p>\n

\"\u76f8\u4f3c\u5ea6\u4f4e\uff0c\u4e5f\u53ef\u80fd\u5b8c\u5168\u5305\u542b\u3002\u5b9e\u9645\u6838\u5bf9\u4ea4\u96c6\u3001\u5e76\u96c6\u4e0e\u67e5\u8be2\u65b9\u5411\u3002\u7247\u6bb5\u627e\u5b8c\u6574\u53c2\u8003\uff0c\u76f8\u4f3c\u5ea6
\u5b9e\u9645\u6838\u5bf9\u4ea4\u96c6\u3001\u5e76\u96c6\u4e0e\u67e5\u8be2\u65b9\u5411<\/figcaption><\/figure>\n

\u8349\u56fe\u4fdd\u7559\u4e86\u4ec0\u4e48\uff0c\u53c8\u7701\u7565\u4e86\u4ec0\u4e48<\/h2>\n

\u5e8f\u5217\u8349\u56fe\u5c06\u5e8f\u5217\u4e2d\u7684\u77ed\u7247\u6bb5\u8f6c\u6362\u4e3a\u54c8\u5e0c\u503c\uff0c\u518d\u6309\u4e00\u5b9a\u89c4\u5219\u4fdd\u7559\u5176\u4e2d\u4e00\u90e8\u5206\uff0c\u7528\u8f83\u5c0f\u7684\u6570\u636e\u8868\u793a\u539f\u59cb\u5e8f\u5217\u5185\u5bb9\u3002\u540e\u7eed\u6bd4\u8f83\u53ef\u4ee5\u56f4\u7ed5\u8fd9\u4e9b\u4fdd\u7559\u503c\u7684\u4ea4\u96c6\u3001\u5e76\u96c6\u548c\u5404\u81ea\u6570\u91cf\u5c55\u5f00\uff0c\u4e0d\u5fc5\u6bcf\u6b21\u91cd\u65b0\u5904\u7406\u6240\u6709\u78b1\u57fa\u3002<\/p>\n

\u8fd9\u79cd\u505a\u6cd5\u9002\u5408\u5927\u91cf\u5e8f\u5217\u7684\u521d\u6b65\u6bd4\u8f83\uff0c\u4e5f\u9700\u8981\u63a5\u53d7\u4fe1\u606f\u62bd\u6837\u5e26\u6765\u7684\u9650\u5236\u3002\u8349\u56fe\u4e2d\u7684\u4e00\u4e2a\u5171\u540c\u503c\u5e76\u4e0d\u662f\u4e00\u6761\u5b8c\u6574\u8bfb\u6bb5\uff0c\u4e00\u7ec4\u5171\u540c\u503c\u4e5f\u4e0d\u662f\u9010\u78b1\u57fa\u6bd4\u5bf9\u7ed3\u679c\u3002\u89e3\u91ca\u8f93\u51fa\u524d\uff0c\u8981\u5148\u77e5\u9053\u4f7f\u7528\u7684\u77ed\u7247\u6bb5\u957f\u5ea6\u3001\u91c7\u6837\u5bc6\u5ea6\u4ee5\u53ca\u662f\u5426\u8bb0\u5f55\u91cd\u590d\u51fa\u73b0\u6b21\u6570\u3002\u5b98\u65b9\u8349\u56fe\u53c2\u6570\u8bf4\u660e<\/a><\/p>\n

\u672c\u6b21\u4e3b\u8981\u4f7f\u7528\u4e09\u5341\u4e00\u4e2a\u78b1\u57fa\u7684\u77ed\u7247\u6bb5\u548c\u4e00\u5343\u7684\u7f29\u653e\u53c2\u6570\uff0c\u6ca1\u6709\u542f\u7528\u4e30\u5ea6\u8ddf\u8e2a\u3002\u6240\u6709\u6bd4\u8f83\u90fd\u4fdd\u5b58\u5b9e\u9645\u8349\u56fe\u6587\u4ef6\uff0c\u9a71\u52a8\u8fd8\u4ece\u6587\u4ef6\u4e2d\u8bfb\u53d6\u54c8\u5e0c\u96c6\u5408\uff0c\u72ec\u7acb\u8ba1\u7b97\u4ea4\u96c6\u548c\u5e76\u96c6\uff0c\u6838\u5bf9\u547d\u4ee4\u884c\u8fd4\u56de\u7684\u6570\u503c\u3002<\/p>\n

\u8f93\u5165\u6765\u81ea\u54ea\u91cc\uff0c\u54ea\u4e9b\u90e8\u5206\u662f\u4eba\u4e3a\u8bbe\u8ba1\u7684<\/h2>\n

\u57fa\u7840\u8f93\u5165\u4e3a\u516c\u5f00\u53c2\u8003\u5e8f\u5217 NC_000913.3\uff0c\u5b9e\u9645\u957f\u5ea6\u4e3a\u56db\u767e\u516d\u5341\u56db\u4e07\u4e00\u5343\u516d\u767e\u4e94\u5341\u4e8c\u78b1\u57fa\u3002\u590d\u73b0\u9a71\u52a8\u68c0\u67e5\u957f\u5ea6\u548c\u5b8c\u6574\u5e8f\u5217\u7684\u6821\u9a8c\u503c\uff0c\u907f\u514d\u4e0d\u540c\u4e0b\u8f7d\u6587\u4ef6\u6216\u4e0d\u540c\u7248\u672c\u88ab\u8bef\u5f53\u6210\u540c\u4e00\u8f93\u5165\u3002NCBI \u53c2\u8003\u5e8f\u5217\u8bb0\u5f55<\/a><\/p>\n

\u6211\u4eec\u4ece\u5b83\u7684\u5f00\u5934\u622a\u53d6\u4e00\u767e\u4e07\u78b1\u57fa\u4f5c\u4e3a\u90e8\u5206\u5e8f\u5217\uff0c\u518d\u622a\u53d6\u4e94\u5343\u78b1\u57fa\u4f5c\u4e3a\u77ed\u7247\u6bb5\u3002\u53e6\u5916\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u751f\u6210\u4e00\u6761\u4e0e\u53c2\u8003\u7b49\u957f\u7684\u4eba\u5de5\u5e8f\u5217\uff0c\u7528\u4e8e\u6784\u6210\u5185\u5bb9\u5df2\u77e5\u7684\u6df7\u5408\u8f93\u5165\u3002<\/p>\n

\u6df7\u5408\u6587\u4ef6\u5305\u542b\u5b8c\u6574\u53c2\u8003\u548c\u4eba\u5de5\u5e8f\u5217\u4e24\u4e2a\u8bb0\u5f55\u3002\u91cd\u590d\u6587\u4ef6\u5219\u628a\u540c\u4e00\u4e2a\u5b8c\u6574\u53c2\u8003\u5199\u4e24\u904d\u3002\u8fd9\u91cc\u7684\u201c\u6df7\u5408\u201d\u53ea\u662f\u4e3a\u4e86\u68c0\u9a8c\u7b97\u6cd5\u800c\u6784\u9020\u7684\u8f93\u5165\uff0c\u6ca1\u6709\u6a21\u62df\u6d4b\u5e8f\u8bef\u5dee\u3001\u8986\u76d6\u4e0d\u5747\u6216\u771f\u5b9e\u7269\u79cd\u4e30\u5ea6\uff0c\u4e5f\u6ca1\u6709\u7ed9\u4eba\u5de5\u5e8f\u5217\u8d4b\u4e88\u5206\u7c7b\u8eab\u4efd\u3002<\/p>\n

\u8fd9\u7ec4\u8bbe\u8ba1\u63d0\u4f9b\u4e86\u6e05\u695a\u7684\u5df2\u77e5\u5173\u7cfb\u3002\u4e00\u767e\u4e07\u78b1\u57fa\u7247\u6bb5\u5b8c\u6574\u6765\u81ea\u53c2\u8003\uff0c\u6df7\u5408\u6587\u4ef6\u5b8c\u6574\u5305\u542b\u53c2\u8003\uff0c\u91cd\u590d\u6587\u4ef6\u589e\u52a0\u4e86\u8bb0\u5f55\u6570\u91cf\u4f46\u6ca1\u6709\u589e\u52a0\u65b0\u7684\u5e8f\u5217\u5185\u5bb9\u3002\u540e\u7eed\u7ed3\u679c\u53ef\u4ee5\u56f4\u7ed5\u8fd9\u4e9b\u5173\u7cfb\u9010\u9879\u68c0\u67e5\u3002<\/p>\n

\u5b89\u88c5\u4e2d\u9047\u5230\u7684\u4f9d\u8d56\u95ee\u9898\u600e\u6837\u5904\u7406<\/h2>\n

\u672c\u6b21\u5728 Python 3.10 \u7684\u72ec\u7acb\u73af\u5883\u4e2d\u5b89\u88c5\uff0c\u5b9e\u9645\u4f7f\u7528\u7684 sourmash \u7248\u672c\u4e3a 4.8.14\u3002\u9996\u6b21\u5b89\u88c5\u5728\u4f9d\u8d56 screed \u7684\u6784\u5efa\u9636\u6bb5\u62a5\u51fa\u7f3a\u5c11 pkg_resources\uff0c\u5c1a\u672a\u8fdb\u5165\u8349\u56fe\u8ba1\u7b97\u3002<\/p>\n

\u6211\u4eec\u5c06\u6784\u5efa\u5de5\u5177\u9650\u5b9a\u5230\u4ecd\u652f\u6301\u8be5\u4f9d\u8d56\u7684\u7248\u672c\u8303\u56f4\uff0c\u5355\u72ec\u5b89\u88c5 screed\uff0c\u518d\u5b89\u88c5\u56fa\u5b9a\u7248\u672c\u7684 sourmash\u3002\u4fee\u590d\u540e\u7248\u672c\u547d\u4ee4\u548c\u4e0b\u9762\u6240\u6709\u5b9e\u9a8c\u5747\u5b8c\u6210\u3002<\/p>\n

python3 -m venv sourmash-env\r\nsource sourmash-env\/bin\/activate\r\npip install "setuptools<81" "setuptools-scm<9" wheel\r\npip install --no-build-isolation screed==1.1.3\r\npip install sourmash==4.8.14\r\nsourmash --version\r\n<\/code><\/pre>\n
\u8fd9\u4e2a\u5904\u7406\u9488\u5bf9\u672c\u6b21\u5b9e\u9645\u9047\u5230\u7684\u6784\u5efa\u517c\u5bb9\u95ee\u9898\u3002\u5176\u4ed6\u73af\u5883\u82e5\u76f4\u63a5\u5b89\u88c5\u6210\u529f\uff0c\u65e0\u987b\u4e3a\u4e86\u590d\u5236\u4e00\u4e2a\u5386\u53f2\u9519\u8bef\u800c\u8c03\u6574\u6784\u5efa\u5de5\u5177\u3002\u4fdd\u7559\u51c6\u786e\u7684\u5f02\u5e38\u9636\u6bb5\u548c\u6700\u7ec8\u7248\u672c\uff0c\u53ef\u4ee5\u5e2e\u52a9\u5224\u65ad\u95ee\u9898\u5c5e\u4e8e\u8f6f\u4ef6\u5b89\u88c5\u8fd8\u662f\u6570\u636e\u5206\u6790\u3002<\/p>\n
\u5148\u751f\u6210\u53ef\u4ee5\u9010\u9879\u6838\u5bf9\u7684\u8349\u56fe<\/h2>\n
\u4e0b\u9762\u4ee5\u5b8c\u6574\u53c2\u8003\u548c\u622a\u53d6\u7247\u6bb5\u4e3a\u4f8b\uff0c\u5206\u522b\u751f\u6210\u8349\u56fe\u3002\u5b9e\u9a8c\u9a71\u52a8\u8fd8\u4f1a\u5bf9\u5176\u4ed6\u8f93\u5165\u6267\u884c\u540c\u6837\u7684\u64cd\u4f5c\u3002<\/p>\n
sourmash sketch dna -p k=31,scaled=1000 \\\r\n  --name-from-first -o full.sig full.fna\r\nsourmash sketch dna -p k=31,scaled=1000 \\\r\n  --name-from-first -o partial.sig partial.fna\r\n<\/code><\/pre>\n
\u5b9e\u9645\u5b8c\u6574\u53c2\u8003\u8349\u56fe\u5305\u542b\u56db\u5343\u56db\u767e\u4e03\u5341\u516d\u4e2a\u54c8\u5e0c\u503c\uff0c\u7247\u6bb5\u8349\u56fe\u5305\u542b\u4e5d\u767e\u4e09\u5341\u4e2a\u3002\u4e8c\u8005\u4ea4\u96c6\u4e5f\u662f\u4e5d\u767e\u4e09\u5341\uff0c\u5e76\u96c6\u4e3a\u56db\u5343\u56db\u767e\u4e03\u5341\u516d\u3002\u8fd9\u610f\u5473\u7740\u672c\u4f8b\u7247\u6bb5\u4fdd\u7559\u4e0b\u6765\u7684\u6240\u6709\u5185\u5bb9\u90fd\u80fd\u591f\u5728\u53c2\u8003\u8349\u56fe\u4e2d\u627e\u5230\u3002<\/p>\n
\u8fd9\u91cc\u4e0d\u80fd\u7528\u539f\u59cb\u5e8f\u5217\u957f\u5ea6\u76f4\u63a5\u66ff\u4ee3\u8349\u56fe\u4e2d\u7684\u96c6\u5408\u6570\u91cf\u3002\u53c2\u8003\u4e2d\u53ef\u80fd\u5b58\u5728\u91cd\u590d\u77ed\u7247\u6bb5\uff0c\u800c\u4e14\u91c7\u6837\u53ea\u4fdd\u7559\u90e8\u5206\u54c8\u5e0c\u503c\u3002\u672c\u6587\u7684\u767e\u5206\u6bd4\u7531\u5b9e\u9645\u8349\u56fe\u96c6\u5408\u8ba1\u7b97\uff0c\u957f\u5ea6\u6bd4\u4f8b\u53ea\u7528\u6765\u5e2e\u52a9\u7406\u89e3\u8f93\u5165\u7684\u5927\u81f4\u89c4\u6a21\u3002<\/p>\n
\u76f8\u4f3c\u5ea6\u548c\u5305\u542b\u5ea6\u4f7f\u7528\u4e86\u4e0d\u540c\u5206\u6bcd<\/h2>\n
\u9ed8\u8ba4\u641c\u7d22\u4f7f\u7528\u7684 Jaccard \u76f8\u4f3c\u5ea6\uff0c\u53ef\u4ee5\u7406\u89e3\u4e3a\u4ea4\u96c6\u5927\u5c0f\u9664\u4ee5\u5e76\u96c6\u5927\u5c0f\u3002\u672c\u4f8b\u4e5d\u767e\u4e09\u5341\u9664\u4ee5\u56db\u5343\u56db\u767e\u4e03\u5341\u516d\uff0c\u5f97\u5230\u7ea6\u767e\u5206\u4e4b\u4e8c\u5341\u70b9\u4e03\u516b\u3002\u7a0b\u5e8f\u5b9e\u9645\u8fd4\u56de 0.2077747989\uff0c\u4e0e\u72ec\u7acb\u8ba1\u7b97\u4e00\u81f4\u3002<\/p>\n
sourmash search partial.sig full.sig \\\r\n  -t 0 -n 0 -o partial_full_jaccard.csv\r\n<\/code><\/pre>\n
\u5982\u679c\u6539\u4e3a\u67e5\u8be2\u5305\u542b\u5ea6\uff0c\u5206\u6bcd\u53d8\u6210\u67e5\u8be2\u8349\u56fe\u81ea\u8eab\u7684\u5927\u5c0f\u3002\u540c\u6837\u7684\u4ea4\u96c6\u4e5d\u767e\u4e09\u5341\uff0c\u9664\u4ee5\u67e5\u8be2\u4e2d\u7684\u4e5d\u767e\u4e09\u5341\uff0c\u7ed3\u679c\u5c31\u662f\u4e00\u3002\u7a0b\u5e8f\u5b9e\u9645\u8fd4\u56de\u767e\u5206\u4e4b\u767e\u3002<\/p>\n
sourmash search partial.sig full.sig \\\r\n  --containment -t 0 -n 0 \\\r\n  -o partial_full_containment.csv\r\n<\/code><\/pre>\n
\u56e0\u6b64\uff0c\u4f4e\u6574\u4f53\u76f8\u4f3c\u5ea6\u4e0e\u5b8c\u5168\u5305\u542b\u53ef\u4ee5\u540c\u65f6\u6210\u7acb\u3002\u5b8c\u6574\u53c2\u8003\u8fd8\u6709\u5927\u91cf\u4e0d\u5728\u7247\u6bb5\u4e2d\u7684\u5185\u5bb9\uff0c\u8fd9\u4e9b\u5185\u5bb9\u4f1a\u6269\u5927\u5e76\u96c6\uff0c\u964d\u4f4e\u6574\u4f53\u76f8\u4f3c\u5ea6\uff0c\u5374\u4e0d\u4f1a\u5426\u5b9a\u67e5\u8be2\u7247\u6bb5\u672c\u8eab\u5df2\u7ecf\u5168\u90e8\u5305\u542b\u5728\u53c2\u8003\u4e2d\u3002<\/p>\n
\u5728\u5b9e\u9645\u4efb\u52a1\u4e2d\uff0c\u5982\u679c\u60f3\u6bd4\u8f83\u4e24\u4efd\u57fa\u56e0\u7ec4\u6574\u4f53\u5185\u5bb9\u662f\u5426\u76f8\u8fd1\uff0c\u53ef\u4ee5\u5148\u8003\u8651\u6574\u4f53\u76f8\u4f3c\u5173\u7cfb\u3002\u5982\u679c\u60f3\u68c0\u67e5\u4e00\u4e2a\u7247\u6bb5\u6216\u57fa\u56e0\u7ec4\u662f\u5426\u51fa\u73b0\u5728\u66f4\u590d\u6742\u7684\u6570\u636e\u4e2d\uff0c\u5c31\u9700\u8981\u7279\u522b\u5173\u6ce8\u5305\u542b\u65b9\u5411\u53ca\u5176\u9002\u7528\u6761\u4ef6\u3002\u5b98\u65b9\u641c\u7d22\u547d\u4ee4\u8bf4\u660e<\/a><\/p>\n
\u628a\u67e5\u8be2\u4e0e\u53c2\u8003\u5bf9\u8c03\uff0c\u4e3a\u4ec0\u4e48\u6570\u5b57\u53c8\u53d8\u5c0f<\/h2>\n
\u6211\u4eec\u5b9e\u9645\u4ea4\u6362\u4e24\u4e2a\u6587\u4ef6\u7684\u4f4d\u7f6e\uff0c\u518d\u6267\u884c\u5305\u542b\u5ea6\u641c\u7d22\u3002\u6b64\u65f6\u67e5\u8be2\u662f\u5b8c\u6574\u53c2\u8003\uff0c\u88ab\u641c\u7d22\u7684\u662f\u4e00\u767e\u4e07\u78b1\u57fa\u7247\u6bb5\uff0c\u7ed3\u679c\u53d8\u6210\u7ea6\u767e\u5206\u4e4b\u4e8c\u5341\u70b9\u4e03\u516b\u3002<\/p>\n
\u4e24\u4efd\u8349\u56fe\u548c\u4ea4\u96c6\u90fd\u6ca1\u6709\u6539\u53d8\uff0c\u6539\u53d8\u7684\u662f\u5206\u6bcd\u3002\u73b0\u5728\u9700\u8981\u56de\u7b54\u5b8c\u6574\u53c2\u8003\u6709\u591a\u5c11\u5185\u5bb9\u51fa\u73b0\u5728\u7247\u6bb5\u91cc\uff0c\u56e0\u6b64\u5206\u6bcd\u4f7f\u7528\u5b8c\u6574\u53c2\u8003\u7684\u56db\u5343\u56db\u767e\u4e03\u5341\u516d\u4e2a\u503c\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n\n\n
\u67e5\u8be2\u5230\u53c2\u8003\u7684\u65b9\u5411<\/th>\n\u4f7f\u7528\u7684\u6307\u6807<\/th>\n\u5b9e\u6d4b\u7ed3\u679c<\/th>\n<\/tr>\n<\/thead>\n
\u7247\u6bb5\u5230\u5b8c\u6574\u53c2\u8003<\/td>\nJaccard \u76f8\u4f3c\u5ea6<\/td>\n20.78%<\/td>\n<\/tr>\n
\u7247\u6bb5\u5230\u5b8c\u6574\u53c2\u8003<\/td>\n\u67e5\u8be2\u5305\u542b\u5ea6<\/td>\n100.00%<\/td>\n<\/tr>\n
\u5b8c\u6574\u53c2\u8003\u5230\u7247\u6bb5<\/td>\n\u67e5\u8be2\u5305\u542b\u5ea6<\/td>\n20.78%<\/td>\n<\/tr>\n
\u5b8c\u6574\u53c2\u8003\u5230\u6df7\u5408\u6587\u4ef6<\/td>\nJaccard \u76f8\u4f3c\u5ea6<\/td>\n48.64%<\/td>\n<\/tr>\n
\u5b8c\u6574\u53c2\u8003\u5230\u6df7\u5408\u6587\u4ef6<\/td>\n\u67e5\u8be2\u5305\u542b\u5ea6<\/td>\n100.00%<\/td>\n<\/tr>\n
\u6df7\u5408\u6587\u4ef6\u5230\u5b8c\u6574\u53c2\u8003<\/td>\n\u67e5\u8be2\u5305\u542b\u5ea6<\/td>\n48.64%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u6574\u7406\u7ed3\u679c\u8868\u65f6\uff0c\u6700\u597d\u540c\u65f6\u4fdd\u7559\u67e5\u8be2\u540d\u79f0\u3001\u53c2\u8003\u540d\u79f0\u548c\u6307\u6807\u7c7b\u578b\u3002\u5982\u679c\u53ea\u5bfc\u51fa\u4e00\u5217\u53eb\u201c\u76f8\u4f3c\u5ea6\u201d\u7684\u6570\u5b57\uff0c\u540e\u7eed\u5f88\u96be\u53d1\u73b0\u6587\u4ef6\u65b9\u5411\u53d1\u751f\u8fc7\u4ea4\u6362\u3002\u6bd4\u8f83\u65b9\u5411\u662f\u8ba1\u7b97\u5b9a\u4e49\u7684\u4e00\u90e8\u5206\uff0c\u5e94\u4e0e\u53c2\u6570\u4e00\u8d77\u8bb0\u5f55\u3002<\/p>\n
\u6df7\u5408\u8f93\u5165\u8ba9\u8fd9\u79cd\u533a\u522b\u66f4\u52a0\u76f4\u89c2<\/h2>\n
\u4eba\u5de5\u5e8f\u5217\u8349\u56fe\u542b\u56db\u5343\u4e03\u767e\u4e8c\u5341\u4e03\u4e2a\u503c\uff0c\u4e0e\u53c2\u8003\u8349\u56fe\u5728\u672c\u6b21\u6761\u4ef6\u4e0b\u6ca1\u6709\u4ea4\u96c6\u3002\u6df7\u5408\u6587\u4ef6\u56e0\u6b64\u5305\u542b\u4e5d\u5343\u4e8c\u767e\u96f6\u4e09\u4e2a\u503c\uff0c\u7b49\u4e8e\u4e24\u4efd\u96c6\u5408\u7684\u5927\u5c0f\u76f8\u52a0\u3002<\/p>\n
\u5b8c\u6574\u53c2\u8003\u4e0e\u6df7\u5408\u6587\u4ef6\u7684 Jaccard \u76f8\u4f3c\u5ea6\u7ea6\u4e3a\u767e\u5206\u4e4b\u56db\u5341\u516b\u70b9\u516d\u56db\uff0c\u56e0\u4e3a\u53c2\u8003\u53ea\u5360\u5e76\u96c6\u7684\u4e00\u90e8\u5206\u3002\u4f46\u4ee5\u5b8c\u6574\u53c2\u8003\u4e3a\u67e5\u8be2\u65f6\uff0c\u5305\u542b\u5ea6\u4ecd\u4e3a\u767e\u5206\u4e4b\u767e\u3002\u53cd\u8fc7\u6765\u4ee5\u6df7\u5408\u6587\u4ef6\u4e3a\u67e5\u8be2\uff0c\u5219\u53ea\u80fd\u7531\u53c2\u8003\u89e3\u91ca\u5176\u4e2d\u7ea6\u767e\u5206\u4e4b\u56db\u5341\u516b\u70b9\u516d\u56db\u7684\u5185\u5bb9\u3002<\/p>\n
\u8fd9\u7ec4\u4e09\u4e2a\u6570\u5b57\u6765\u81ea\u540c\u4e00\u7ec4\u8f93\u5165\uff0c\u5206\u522b\u56de\u7b54\u4e0d\u540c\u95ee\u9898\u3002\u4e0d\u80fd\u628a\u767e\u5206\u4e4b\u767e\u5305\u542b\u76f4\u63a5\u5199\u6210\u201c\u6837\u672c\u53ea\u6709\u8fd9\u4e2a\u7269\u79cd\u201d\uff0c\u4e5f\u4e0d\u80fd\u628a\u63a5\u8fd1\u4e00\u534a\u7684\u6574\u4f53\u76f8\u4f3c\u5ea6\u76f4\u63a5\u89e3\u91ca\u4e3a\u201c\u53c2\u8003\u6709\u4e00\u534a\u4f4d\u7f6e\u53d1\u751f\u4e86\u7a81\u53d8\u201d\u3002<\/p>\n
\u672c\u6b21\u8fd8\u5b9e\u9645\u8fd0\u884c\u6df7\u5408\u5206\u89e3\uff0c\u8ba9\u7a0b\u5e8f\u5728\u5b8c\u6574\u53c2\u8003\u4e0e\u4eba\u5de5\u5e8f\u5217\u4e24\u4e2a\u5019\u9009\u4e4b\u95f4\u89e3\u91ca\u6df7\u5408\u8349\u56fe\u3002<\/p>\n
sourmash gather mixture.sig full.sig unrelated.sig \\\r\n  --threshold-bp 10000 -o gather.csv\r\n<\/code><\/pre>\n
\u8f93\u51fa\u4e2d\u4e24\u4efd\u8f93\u5165\u5206\u522b\u89e3\u91ca\u7ea6\u767e\u5206\u4e4b\u56db\u5341\u516b\u70b9\u516d\u56db\u548c\u767e\u5206\u4e4b\u4e94\u5341\u4e00\u70b9\u4e09\u516d\u7684\u67e5\u8be2\u5185\u5bb9\uff0c\u5269\u4f59\u672a\u89e3\u91ca\u90e8\u5206\u4e3a\u96f6\u3002\u8fd9\u4e9b\u6bd4\u4f8b\u6765\u81ea\u672c\u6b21\u672a\u8ddf\u8e2a\u4e30\u5ea6\u7684\u8349\u56fe\uff0c\u4e0d\u662f\u6839\u636e\u8bfb\u6bb5\u8986\u76d6\u8ba1\u7b97\u7684\u7269\u79cd\u76f8\u5bf9\u4e30\u5ea6\u3002\u4e24\u6761\u539f\u59cb\u5e8f\u5217\u867d\u7b49\u957f\uff0c\u91c7\u6837\u540e\u7684\u96c6\u5408\u6570\u91cf\u4e5f\u5e76\u975e\u5fc5\u987b\u5b8c\u5168\u76f8\u7b49\u3002<\/p>\n
\u91cd\u590d\u5199\u4e24\u904d\u53c2\u8003\uff0c\u4f1a\u8ba9\u76f8\u4f3c\u5ea6\u7ffb\u500d\u5417<\/h2>\n
\u6211\u4eec\u628a\u540c\u4e00\u4efd\u5b8c\u6574\u53c2\u8003\u5199\u6210\u4e24\u4e2a\u8bb0\u5f55\uff0c\u91cd\u65b0\u751f\u6210\u8349\u56fe\u3002\u5b9e\u9645\u4ecd\u4fdd\u7559\u56db\u5343\u56db\u767e\u4e03\u5341\u516d\u4e2a\u54c8\u5e0c\u503c\uff0c\u4e0e\u5355\u4efd\u53c2\u8003\u4e00\u81f4\uff0c\u6bd4\u8f83\u7ed3\u679c\u4e3a\u4e00\u3002<\/p>\n
\u539f\u56e0\u662f\u672c\u6b21\u4f7f\u7528\u7684\u662f\u4e0d\u8ddf\u8e2a\u4e30\u5ea6\u7684\u96c6\u5408\u3002\u540c\u4e00\u77ed\u7247\u6bb5\u51fa\u73b0\u66f4\u591a\u6b21\uff0c\u4e0d\u4f1a\u56e0\u6b64\u6210\u4e3a\u65b0\u7684\u96c6\u5408\u6210\u5458\u3002\u8fd9\u6709\u52a9\u4e8e\u6bd4\u8f83\u5e8f\u5217\u5185\u5bb9\uff0c\u4e5f\u8bf4\u660e\u8be5\u8bbe\u7f6e\u4e0d\u80fd\u8868\u8fbe\u8fd9\u6761\u5e8f\u5217\u5728\u8f93\u5165\u91cc\u51fa\u73b0\u4e86\u591a\u5c11\u904d\u3002<\/p>\n
\u5904\u7406\u771f\u5b9e\u6d4b\u5e8f\u6570\u636e\u65f6\uff0c\u9700\u8981\u6839\u636e\u4efb\u52a1\u51b3\u5b9a\u662f\u5426\u8ddf\u8e2a\u4e30\u5ea6\u3002\u82e5\u5173\u5fc3\u91cd\u590d\u51fa\u73b0\u6b21\u6570\u6216\u8986\u76d6\u76f8\u5173\u4fe1\u606f\uff0c\u5c31\u5e94\u660e\u786e\u68c0\u67e5\u76f8\u5173\u8bbe\u7f6e\u4e0e\u4e0b\u6e38\u6307\u6807\u3002\u4e0d\u80fd\u4e00\u8fb9\u4f7f\u7528\u65e0\u4e30\u5ea6\u8349\u56fe\uff0c\u4e00\u8fb9\u628a\u7ed3\u679c\u5f53\u6210\u8bfb\u6bb5\u6570\u91cf\u7684\u7cbe\u786e\u6c47\u603b\u3002<\/p>\n
\u7f29\u653e\u53c2\u6570\u53d8\u5927\uff0c\u77ed\u7247\u6bb5\u53ef\u80fd\u6ca1\u6709\u7559\u4e0b\u4efb\u4f55\u503c<\/h2>\n
\u6211\u4eec\u5bf9\u4e00\u767e\u4e07\u78b1\u57fa\u7247\u6bb5\u6539\u7528\u4e00\u4e07\u7684\u7f29\u653e\u53c2\u6570\uff0c\u5b9e\u9645\u4fdd\u7559\u4e5d\u5341\u4e5d\u4e2a\u54c8\u5e0c\u503c\u3002\u4e0e\u4e00\u5343\u7f29\u653e\u7684\u5b8c\u6574\u53c2\u8003\u8fdb\u884c\u517c\u5bb9\u6bd4\u8f83\u65f6\uff0c\u7a0b\u5e8f\u5728\u5171\u540c\u91c7\u6837\u5c3a\u5ea6\u4e0a\u8ba1\u7b97\uff0c\u5305\u542b\u5ea6\u4ecd\u7136\u4e3a\u4e00\u3002<\/p>\n
\u4f46\u66f4\u7a00\u758f\u7684\u8349\u56fe\u4f1a\u964d\u4f4e\u7528\u4e8e\u4f30\u8ba1\u7684\u8bc1\u636e\u6570\u91cf\u3002\u5bf9\u4e94\u5343\u78b1\u57fa\u77ed\u7247\u6bb5\uff0c\u672c\u6b21\u4e00\u5343\u7f29\u653e\u4fdd\u7559\u56db\u4e2a\u503c\uff0c\u5341\u4e07\u7f29\u653e\u5219\u4e00\u4e2a\u90fd\u6ca1\u6709\u7559\u4e0b\u3002\u8fd9\u4e2a\u7a7a\u8349\u56fe\u7531\u91c7\u6837\u548c\u8f93\u5165\u957f\u5ea6\u5171\u540c\u9020\u6210\uff0c\u4e0d\u80fd\u88ab\u89e3\u91ca\u4e3a\u8be5\u7247\u6bb5\u6ca1\u6709\u5e8f\u5217\u6216\u6ca1\u6709\u751f\u7269\u5b66\u6765\u6e90\u3002<\/p>\n
\u5982\u679c\u5c0f\u7247\u6bb5\u3001\u4f4e\u8986\u76d6\u6216\u7a00\u6709\u5185\u5bb9\u662f\u7814\u7a76\u91cd\u70b9\uff0c\u5efa\u8bae\u5148\u68c0\u67e5\u5b9e\u9645\u8349\u56fe\u5927\u5c0f\uff0c\u518d\u51b3\u5b9a\u91c7\u6837\u8bbe\u7f6e\u3002\u8f93\u51fa\u6587\u4ef6\u5b58\u5728\u5e76\u4e0d\u4fdd\u8bc1\u5176\u4e2d\u4fdd\u7559\u4e86\u8db3\u591f\u4fe1\u606f\u3002\u53ea\u5bf9\u6587\u4ef6\u5927\u5c0f\u6216\u4efb\u52a1\u9000\u51fa\u72b6\u6001\u505a\u68c0\u67e5\uff0c\u53ef\u80fd\u6f0f\u6389\u8fd9\u79cd\u60c5\u51b5\u3002<\/p>\n
\u7f29\u653e\u4e5f\u4e0d\u662f\u8d8a\u5c0f\u8d8a\u597d\u3002\u66f4\u5bc6\u7684\u8349\u56fe\u9700\u8981\u4fdd\u5b58\u548c\u6bd4\u8f83\u66f4\u591a\u503c\uff0c\u5b9e\u9645\u9009\u62e9\u5e94\u7ed3\u5408\u7814\u7a76\u76ee\u6807\u548c\u6570\u636e\u89c4\u6a21\u3002\u672c\u6b21\u53ea\u662f\u5c55\u793a\u4e00\u4e2a\u53ef\u590d\u7b97\u7684\u53d6\u820d\uff0c\u6ca1\u6709\u7528\u51e0\u4efd\u5c0f\u8f93\u5165\u63a8\u5bfc\u5927\u89c4\u6a21\u6570\u636e\u7684\u541e\u5410\u91cf\u3002<\/p>\n
\u4e0d\u540c\u77ed\u7247\u6bb5\u957f\u5ea6\u4f1a\u76f4\u63a5\u9020\u6210\u4e0d\u517c\u5bb9<\/h2>\n
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