{"id":72812,"date":"2026-11-15T11:16:12","date_gmt":"2026-11-15T03:16:12","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72812"},"modified":"2026-11-15T11:16:12","modified_gmt":"2026-11-15T03:16:12","slug":"skani-ani-aligned-fraction-query-reference-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/skani-ani-aligned-fraction-query-reference-practice\/","title":{"rendered":"skani \u7b97\u51fa 100% ANI\uff0c\u80fd\u8bf4\u4e24\u4efd\u57fa\u56e0\u7ec4\u4e00\u6837\u5417\uff1f\u5b9e\u8dd1\u68c0\u67e5\u53cc\u5411\u8986\u76d6\u4e0e\u7ed3\u679c\u8fc7\u6ee4"},"content":{"rendered":"
\n

\u6bd4\u8f83\u7ec6\u83cc\u57fa\u56e0\u7ec4\u6216 MAG \u65f6\uff0c\u770b\u5230\u63a5\u8fd1\u767e\u5206\u4e4b\u767e\u7684 ANI \u5f88\u5bb9\u6613\u4ea7\u751f\u4e00\u79cd\u5370\u8c61\uff0c\u4e24\u4efd\u5e8f\u5217\u51e0\u4e4e\u5b8c\u5168\u76f8\u540c\u3002\u4f46 ANI \u63cf\u8ff0\u7684\u662f\u53ef\u6bd4\u8f83\u90e8\u5206\u7684\u6838\u82f7\u9178\u4e00\u81f4\u7a0b\u5ea6\uff0c\u8fd8\u9700\u8981\u7ed3\u5408\u6bd4\u8f83\u8986\u76d6\u4e86\u53cc\u65b9\u591a\u5927\u8303\u56f4\u6765\u89e3\u91ca\u3002<\/p>\n

\u672c\u6587\u5b89\u88c5\u5e76\u5b9e\u9645\u8fd0\u884c skani 0.4.0\uff0c\u7528\u4e00\u4efd\u516c\u5f00\u53c2\u8003\u6784\u9020\u5b8c\u6574\u3001\u622a\u53d6\u3001\u7a81\u53d8\u548c\u6df7\u5408\u56db\u7c7b\u8f93\u5165\u3002\u6211\u4eec\u540c\u65f6\u68c0\u67e5 ANI\u3001\u53c2\u8003\u8986\u76d6\u6bd4\u4f8b\u3001\u67e5\u8be2\u8986\u76d6\u6bd4\u4f8b\uff0c\u4ee5\u53ca\u4e24\u4e2a\u8986\u76d6\u8fc7\u6ee4\u9009\u9879\u7684\u4e0d\u540c\u542b\u4e49\u3002\u5b9e\u9a8c\u4e2d\u7279\u610f\u4fdd\u7559\u4e86\u9ad8 ANI \u4f46\u8986\u76d6\u8f83\u5c11\u7684\u7ed3\u679c\uff0c\u8ba9\u8bfb\u8005\u80fd\u591f\u770b\u5230\u4ec5\u62a5\u544a\u4e00\u4e2a\u6570\u5b57\u4f1a\u9057\u6f0f\u4ec0\u4e48\u3002<\/p>\n

\"\u767e\u5206\u4e4b\u767e
\u5b9e\u9645\u6bd4\u8f83\u5b8c\u6574\u3001\u5c40\u90e8\u548c\u6df7\u5408\u8f93\u5165<\/figcaption><\/figure>\n

\u5148\u628a ANI \u548c\u8986\u76d6\u8303\u56f4\u653e\u5728\u540c\u4e00\u884c<\/h2>\n

\u5f53\u4e24\u4efd\u5e8f\u5217\u53ea\u6709\u4e00\u90e8\u5206\u5185\u5bb9\u80fd\u591f\u5efa\u7acb\u6bd4\u8f83\u5173\u7cfb\u65f6\uff0c\u53ef\u4ee5\u5728\u8fd9\u90e8\u5206\u5185\u5bb9\u4e2d\u5f97\u5230\u5f88\u9ad8\u7684\u4e00\u81f4\u7a0b\u5ea6\uff0c\u4f46\u5176\u4f59\u5185\u5bb9\u5e76\u6ca1\u6709\u56e0\u6b64\u88ab\u8bc1\u660e\u76f8\u540c\u3002\u5bf9\u4e0d\u5b8c\u6574\u7ec4\u88c5\u3001\u5c40\u90e8\u7247\u6bb5\u548c\u6df7\u5408\u8f93\u5165\uff0c\u8fd9\u79cd\u60c5\u51b5\u5c24\u5176\u503c\u5f97\u68c0\u67e5\u3002<\/p>\n

skani \u4f1a\u540c\u65f6\u8f93\u51fa ANI \u4e0e\u4e24\u4e2a\u65b9\u5411\u7684\u5bf9\u9f50\u6bd4\u4f8b\u3002\u53c2\u8003\u6bd4\u4f8b\u7684\u5206\u6bcd\u5bf9\u5e94\u53c2\u8003\uff0c\u67e5\u8be2\u6bd4\u4f8b\u7684\u5206\u6bcd\u5bf9\u5e94\u67e5\u8be2\u3002\u4ea4\u6362\u8f93\u5165\u65b9\u5411\u540e\uff0c\u9700\u8981\u91cd\u65b0\u786e\u8ba4\u6bcf\u4e00\u5217\u6240\u6307\u5411\u7684\u5bf9\u8c61\uff0c\u4e0d\u80fd\u628a\u5217\u540d\u7701\u7565\u6210\u4e00\u4e2a\u542b\u7cca\u7684\u201c\u8986\u76d6\u7387\u201d\u3002<\/p>\n

\u8f6f\u4ef6\u91c7\u7528\u8fd1\u4f3c\u6620\u5c04\u548c\u8349\u56fe\u7b5b\u9009\u6765\u4f30\u8ba1\u76f8\u5173\u6307\u6807\uff0c\u9002\u5408\u7528\u4e8e\u5927\u91cf\u57fa\u56e0\u7ec4\u7684\u6bd4\u8f83\u4e0e\u5019\u9009\u7b5b\u67e5\u3002\u5b83\u63d0\u4f9b\u7684\u6c47\u603b\u7ed3\u679c\u4e5f\u4e0d\u7b49\u540c\u4e8e\u9010\u78b1\u57fa\u6bd4\u5bf9\u6587\u4ef6\uff0c\u82e5\u8981\u5b9a\u4f4d\u7a81\u53d8\u6216\u7ed3\u6784\u5dee\u5f02\uff0c\u4ecd\u9700\u8981\u76f8\u5e94\u7684\u540e\u7eed\u5206\u6790\u3002skani \u5b98\u65b9\u9879\u76ee\u8bf4\u660e<\/a><\/p>\n

\u672c\u6b21\u5b89\u88c5\u548c\u7248\u672c\u5982\u4f55\u56fa\u5b9a<\/h2>\n

\u672c\u6b21\u4e0b\u8f7d\u9879\u76ee\u63d0\u4f9b\u7684 Linux x86_64 \u9759\u6001\u53ef\u6267\u884c\u6587\u4ef6\uff0c\u8d4b\u4e88\u6267\u884c\u6743\u9650\u540e\u76f4\u63a5\u8fd0\u884c\u3002\u5b9e\u9645\u7248\u672c\u8f93\u51fa\u4e3a 0.4.0\uff0c\u4e0b\u8f7d\u5305\u8bb0\u5f55\u4e86\u6587\u4ef6\u6821\u9a8c\u503c\uff0c\u6ca1\u6709\u4ec5\u51ed\u4e0b\u8f7d\u94fe\u63a5\u4e2d\u7684\u540d\u79f0\u5224\u65ad\u7248\u672c\u3002<\/p>\n

curl -fL \\\r\n  https:\/\/github.com\/bluenote-1577\/skani\/releases\/download\/latest\/skani \\\r\n  -o skani\r\nchmod +x skani\r\n.\/skani --version\r\nsha256sum skani\r\n<\/code><\/pre>\n
\u9700\u8981\u6ce8\u610f\uff0c\u5e26\u6709 latest \u7684\u4e0b\u8f7d\u5730\u5740\u53ef\u80fd\u968f\u53d1\u5e03\u66f4\u65b0\u3002\u590d\u73b0\u5b9e\u9a8c\u5e94\u6838\u5bf9\u5b9e\u9645\u8fd4\u56de\u7248\u672c\u548c\u6821\u9a8c\u503c\uff0c\u4e0d\u80fd\u5047\u5b9a\u672a\u6765\u4ece\u76f8\u540c\u5730\u5740\u4e0b\u8f7d\u7684\u6587\u4ef6\u4ecd\u7136\u76f8\u540c\u3002\u672c\u6b21\u6240\u6709\u5bf9\u7167\u4f7f\u7528\u540c\u4e00\u4e2a\u53ef\u6267\u884c\u6587\u4ef6\uff0c\u6bcf\u6b21\u8c03\u7528\u9650\u5236\u4e3a\u4e24\u4e2a\u7ebf\u7a0b\u3002<\/p>\n
\u5b98\u65b9\u53d1\u5e03\u8bf4\u660e\u8fd8\u8bb0\u5f55\u4e86\u96f6\u70b9\u56db\u7248\u672c\u7684\u8349\u56fe\u683c\u5f0f\u53d8\u5316\u3002\u5982\u679c\u9879\u76ee\u4e2d\u4fdd\u5b58\u4e86\u65e7\u7248\u672c\u8349\u56fe\u6216\u9884\u5efa\u6570\u636e\u5e93\uff0c\u5347\u7ea7\u65f6\u5e94\u6309\u53d1\u5e03\u8bf4\u660e\u6838\u5bf9\u517c\u5bb9\u6027\u3002\u672c\u6b21\u76f4\u63a5\u8bfb\u53d6 FASTA\uff0c\u6ca1\u6709\u501f\u7528\u8de8\u7248\u672c\u8349\u56fe\u5e93\u6765\u89e3\u91ca\u7ed3\u679c\u30020.4.0 \u7248\u672c\u53d1\u5e03\u8bf4\u660e<\/a><\/p>\n
\u8f93\u5165\u662f\u4e00\u4efd\u53c2\u8003\u548c\u51e0\u7ec4\u660e\u786e\u7684\u6539\u52a8<\/h2>\n
\u57fa\u7840\u53c2\u8003\u4e3a\u516c\u5f00\u5e8f\u5217 NC_000913.3\uff0c\u5b9e\u9645\u957f\u5ea6\u4e3a\u56db\u767e\u516d\u5341\u56db\u4e07\u4e00\u5343\u516d\u767e\u4e94\u5341\u4e8c\u78b1\u57fa\u3002\u9a71\u52a8\u4f1a\u68c0\u67e5\u5e8f\u5217\u957f\u5ea6\u548c\u6821\u9a8c\u503c\uff0c\u518d\u751f\u6210\u5176\u4ed6\u8f93\u5165\uff0c\u4fdd\u8bc1\u6bd4\u8f83\u7684\u662f\u540c\u4e00\u7248\u672c\u7684\u53c2\u8003\u3002NCBI \u53c2\u8003\u5e8f\u5217\u8bb0\u5f55<\/a><\/p>\n
\u7b2c\u4e00\u7ec4\u76f4\u63a5\u4f7f\u7528\u5b8c\u6574\u53c2\u8003\uff0c\u4f5c\u4e3a\u81ea\u8eab\u6bd4\u8f83\u3002\u7b2c\u4e8c\u7ec4\u622a\u53d6\u5f00\u5934\u4e00\u767e\u4e07\u78b1\u57fa\uff0c\u7b2c\u4e09\u7ec4\u8fdb\u4e00\u6b65\u622a\u53d6\u5f00\u5934\u5341\u4e07\u78b1\u57fa\uff0c\u7528\u4e8e\u89c2\u5bdf\u540c\u4e00\u5185\u5bb9\u5728\u4e0d\u540c\u957f\u5ea6\u5206\u6bcd\u4e0b\u7684\u8868\u73b0\u3002<\/p>\n
\u7b2c\u56db\u7ec4\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\uff0c\u5728\u5b8c\u6574\u53c2\u8003\u4e2d\u9009\u62e9\u56db\u4e07\u516d\u5343\u56db\u767e\u4e00\u5341\u516d\u4e2a\u4f4d\u7f6e\uff0c\u5e76\u628a\u6bcf\u4e2a\u4f4d\u7f6e\u7684\u78b1\u57fa\u66ff\u6362\u4e3a\u53e6\u4e00\u79cd\u78b1\u57fa\u3002\u5b83\u4e0e\u539f\u53c2\u8003\u7684\u8bbe\u8ba1\u5168\u957f\u9010\u4f4d\u4e00\u81f4\u7387\u7ea6\u4e3a\u767e\u5206\u4e4b\u4e5d\u5341\u4e5d\u3002<\/p>\n
\u7b2c\u4e94\u7ec4\u4fdd\u7559\u53c2\u8003\u5f00\u5934\u7684\u4e00\u767e\u4e07\u78b1\u57fa\uff0c\u518d\u52a0\u5165\u4e00\u6761\u4eba\u5de5\u751f\u6210\u7684\u5e8f\u5217\uff0c\u4f7f\u6587\u4ef6\u603b\u957f\u5ea6\u4e0e\u539f\u53c2\u8003\u76f8\u540c\u3002\u4e24\u90e8\u5206\u5206\u522b\u5199\u6210\u4e24\u4e2a\u5e8f\u5217\u8bb0\u5f55\u3002\u5b83\u7528\u4e8e\u5c55\u793a\u201c\u6587\u4ef6\u4e00\u6837\u957f\u201d\u4e5f\u4e0d\u4ee3\u8868\u6240\u6709\u5185\u5bb9\u90fd\u80fd\u6bd4\u8f83\uff0c\u6ca1\u6709\u6a21\u62df\u771f\u5b9e\u7fa4\u843d\u6216\u771f\u5b9e\u6c61\u67d3\u7387\u3002<\/p>\n
\u81ea\u8eab\u6bd4\u8f83\u5148\u786e\u8ba4\u57fa\u672c\u8f93\u5165\u8f93\u51fa<\/h2>\n
\u6211\u4eec\u5148\u8ba9\u5b8c\u6574\u53c2\u8003\u4e0e\u81ea\u8eab\u6bd4\u8f83\uff0c\u5e76\u4fdd\u5b58\u8be6\u7ec6\u8f93\u51fa\u3002\u547d\u4ee4\u4e2d\u7684\u67e5\u8be2\u548c\u53c2\u8003\u9009\u9879\u660e\u786e\u6307\u5b9a\u65b9\u5411\uff0c\u7ed3\u679c\u5199\u4e3a\u5236\u8868\u7b26\u5206\u9694\u6587\u4ef6\u3002<\/p>\n
.\/skani dist \\\r\n  -q full.fna -r full.fna \\\r\n  -o full_self.tsv -t 2 --detailed\r\n<\/code><\/pre>\n
\u5b9e\u9645 ANI \u4e3a\u767e\u5206\u4e4b\u767e\uff0c\u53c2\u8003\u548c\u67e5\u8be2\u5bf9\u9f50\u6bd4\u4f8b\u4e5f\u90fd\u662f\u767e\u5206\u4e4b\u767e\u3002\u8fd9\u4e2a\u5bf9\u7167\u8868\u660e\u672c\u6b21\u8f93\u5165\u80fd\u591f\u88ab\u7a0b\u5e8f\u6b63\u5e38\u8bfb\u53d6\uff0c\u57fa\u672c\u6bd4\u8f83\u8fd4\u56de\u4e86\u7b26\u5408\u8bbe\u8ba1\u7684\u7ed3\u679c\u3002<\/p>\n
\u81ea\u8eab\u6bd4\u8f83\u4e0d\u80fd\u8bc1\u660e\u540e\u9762\u6bcf\u79cd\u6570\u636e\u90fd\u80fd\u591f\u51c6\u786e\u4f30\u8ba1\uff0c\u4f46\u53ef\u4ee5\u5e2e\u52a9\u53d1\u73b0\u66f4\u57fa\u7840\u7684\u95ee\u9898\u3002\u4f8b\u5982\u6587\u4ef6\u662f\u5426\u8bfb\u9519\u3001\u5e8f\u5217\u662f\u5426\u4e3a\u7a7a\uff0c\u6216\u8005\u8c03\u7528\u7684\u53ef\u6267\u884c\u7a0b\u5e8f\u662f\u5426\u4e0e\u8bb0\u5f55\u4e00\u81f4\u3002\u5148\u4fdd\u7559\u8fd9\u6837\u4e00\u4efd\u7b80\u5355\u5bf9\u7167\uff0c\u901a\u5e38\u6bd4\u76f4\u63a5\u4ece\u590d\u6742\u7ec4\u88c5\u7684\u5f02\u5e38\u5206\u6570\u5f00\u59cb\u731c\u6d4b\u66f4\u5bb9\u6613\u5b9a\u4f4d\u539f\u56e0\u3002<\/p>\n
\u4e00\u767e\u4e07\u78b1\u57fa\u7247\u6bb5\u7684 ANI \u4e3a\u4ec0\u4e48\u4e5f\u662f\u767e\u5206\u4e4b\u767e<\/h2>\n
\u63a5\u4e0b\u6765\u4ee5\u4e00\u767e\u4e07\u78b1\u57fa\u7247\u6bb5\u4e3a\u67e5\u8be2\uff0c\u4ee5\u5b8c\u6574\u53c2\u8003\u4e3a\u53c2\u8003\u3002\u5b9e\u9645 ANI \u4ecd\u7136\u4e3a\u767e\u5206\u4e4b\u767e\uff0c\u4f46\u53c2\u8003\u5bf9\u9f50\u6bd4\u4f8b\u53ea\u6709\u767e\u5206\u4e4b\u4e8c\u5341\u4e00\u70b9\u4e94\u516b\uff0c\u67e5\u8be2\u5bf9\u9f50\u6bd4\u4f8b\u4e3a\u767e\u5206\u4e4b\u767e\u3002<\/p>\n
.\/skani dist \\\r\n  -q partial.fna -r full.fna \\\r\n  -o partial_full.tsv -t 2 --detailed\r\n<\/code><\/pre>\n
\u8fd9\u4e0e\u8f93\u5165\u8bbe\u8ba1\u76f8\u7b26\u3002\u7247\u6bb5\u5b8c\u5168\u4ece\u53c2\u8003\u622a\u53d6\uff0c\u56e0\u6b64\u81ea\u8eab\u5185\u5bb9\u53ef\u4ee5\u5f97\u5230\u9ad8\u5ea6\u4e00\u81f4\u7684\u6bd4\u8f83\u5173\u7cfb\uff0c\u4f46\u5b83\u53ea\u5360\u5b8c\u6574\u53c2\u8003\u7684\u4e00\u90e8\u5206\u3002\u4e0d\u80fd\u56e0\u4e3a ANI \u8fbe\u5230\u767e\u5206\u4e4b\u767e\uff0c\u5c31\u628a\u5c1a\u672a\u51fa\u73b0\u5728\u67e5\u8be2\u4e2d\u7684\u5176\u4ed6\u53c2\u8003\u533a\u57df\u4e5f\u8ba4\u5b9a\u4e3a\u4e00\u81f4\u3002<\/p>\n
\u7136\u540e\u6211\u4eec\u628a\u4e24\u4e2a\u8f93\u5165\u5bf9\u8c03\u3002\u5b9e\u9645 ANI \u4ecd\u4e3a\u767e\u5206\u4e4b\u767e\uff0c\u4e24\u4e2a\u8986\u76d6\u6570\u5b57\u4ea4\u6362\u4f4d\u7f6e\uff0c\u53c2\u8003\u6bd4\u4f8b\u53d8\u6210\u767e\u5206\u4e4b\u767e\uff0c\u67e5\u8be2\u6bd4\u4f8b\u53d8\u6210\u767e\u5206\u4e4b\u4e8c\u5341\u4e00\u70b9\u4e94\u516b\u3002\u8fd9\u6837\u5c31\u80fd\u901a\u8fc7\u540c\u4e00\u7ec4\u6570\u636e\u68c0\u67e5\u65b9\u5411\u89e3\u91ca\u662f\u5426\u6b63\u786e\u3002<\/p>\n
\u5728\u6574\u7406\u6279\u91cf\u7ed3\u679c\u65f6\uff0c\u5efa\u8bae\u4fdd\u7559\u67e5\u8be2\u6587\u4ef6\u540d\u548c\u53c2\u8003\u6587\u4ef6\u540d\u3002\u82e5\u628a\u4e24\u4e2a\u65b9\u5411\u6df7\u5728\u4e00\u8d77\uff0c\u53ea\u4fdd\u7559\u67d0\u4e00\u5217\u8986\u76d6\u7387\uff0c\u5c31\u53ef\u80fd\u5bf9\u540c\u4e00\u5bf9\u5e8f\u5217\u5f97\u51fa\u770b\u4f3c\u77db\u76fe\u7684\u7ed3\u8bba\u3002<\/p>\n
\u540c\u6837\u957f\u5ea6\u7684\u6df7\u5408\u6587\u4ef6\uff0c\u4e5f\u53ef\u4ee5\u5f97\u5230\u9ad8 ANI<\/h2>\n
\u6df7\u5408\u8f93\u5165\u7684\u603b\u957f\u5ea6\u4e0e\u5b8c\u6574\u53c2\u8003\u76f8\u540c\uff0c\u4f46\u53ea\u6709\u5176\u4e2d\u4e00\u767e\u4e07\u78b1\u57fa\u76f4\u63a5\u6765\u81ea\u53c2\u8003\uff0c\u5176\u4f59\u662f\u72ec\u7acb\u751f\u6210\u7684\u4eba\u5de5\u5e8f\u5217\u3002\u5b9e\u9645\u6bd4\u8f83\u4ecd\u5f97\u5230\u767e\u5206\u4e4b\u767e ANI\uff0c\u800c\u4e24\u8fb9\u5bf9\u9f50\u6bd4\u4f8b\u90fd\u53ea\u6709\u7ea6\u767e\u5206\u4e4b\u4e8c\u5341\u4e00\u70b9\u4e94\u4e5d\u3002<\/p>\n
\u8fd9\u6b21\u53cc\u65b9\u957f\u5ea6\u76f8\u540c\uff0c\u6240\u4ee5\u4e24\u4e2a\u65b9\u5411\u7684\u6bd4\u4f8b\u63a5\u8fd1\u4e00\u81f4\u3002\u4f46\u5927\u90e8\u5206\u5185\u5bb9\u5e76\u6ca1\u6709\u5efa\u7acb\u53ef\u7528\u4e8e\u8be5\u7ed3\u679c\u7684\u6bd4\u8f83\u5173\u7cfb\uff0c\u9ad8 ANI \u4ecd\u7136\u53ea\u63cf\u8ff0\u53ef\u6bd4\u8f83\u90e8\u5206\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n\n
\u67e5\u8be2\u8f93\u5165<\/th>\nANI<\/th>\n\u53c2\u8003\u5bf9\u9f50\u6bd4\u4f8b<\/th>\n\u67e5\u8be2\u5bf9\u9f50\u6bd4\u4f8b<\/th>\n<\/tr>\n<\/thead>\n
\u5b8c\u6574\u53c2\u8003\u81ea\u8eab<\/td>\n100.00%<\/td>\n100.00%<\/td>\n100.00%<\/td>\n<\/tr>\n
\u4e00\u767e\u4e07\u78b1\u57fa\u7247\u6bb5<\/td>\n100.00%<\/td>\n21.58%<\/td>\n100.00%<\/td>\n<\/tr>\n
\u5341\u4e07\u78b1\u57fa\u7247\u6bb5<\/td>\n100.00%<\/td>\n2.15%<\/td>\n100.00%<\/td>\n<\/tr>\n
\u8bbe\u8ba1\u7a81\u53d8\u7684\u5b8c\u6574\u5e8f\u5217<\/td>\n98.92%<\/td>\n99.83%<\/td>\n99.83%<\/td>\n<\/tr>\n
\u7b49\u957f\u6df7\u5408\u6587\u4ef6<\/td>\n100.00%<\/td>\n21.59%<\/td>\n21.59%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8fd9\u7ec4\u5bf9\u7167\u8bf4\u660e\uff0c\u6587\u4ef6\u603b\u957f\u5ea6\u3001ANI \u548c\u53cc\u5411\u8986\u76d6\u9700\u8981\u4e00\u8d77\u770b\u3002\u603b\u957f\u5ea6\u76f8\u8fd1\u4e0d\u80fd\u4ee3\u66ff\u5185\u5bb9\u5339\u914d\uff0cANI \u5f88\u9ad8\u4e5f\u4e0d\u80fd\u4ee3\u66ff\u5e7f\u6cdb\u8986\u76d6\u3002\u5c06\u8fd9\u51e0\u4e2a\u6570\u5b57\u653e\u5728\u4e00\u884c\uff0c\u6bd4\u5355\u72ec\u6309 ANI \u6392\u5e8f\u66f4\u5bb9\u6613\u8bc6\u522b\u9700\u8981\u7ee7\u7eed\u68c0\u67e5\u7684\u5bf9\u8c61\u3002<\/p>\n
\u5bf9\u4e8e\u771f\u5b9e MAG\uff0c\u5982\u679c\u53d1\u73b0\u9ad8 ANI \u4f46\u53cc\u65b9\u8986\u76d6\u90fd\u4f4e\uff0c\u53ef\u4ee5\u8fdb\u4e00\u6b65\u67e5\u770b\u7ec4\u88c5\u7ec4\u6210\u3001\u5171\u4eab\u7247\u6bb5\u3001\u53c2\u8003\u9009\u62e9\u548c\u5206\u7bb1\u7ed3\u679c\u3002\u672c\u4f8b\u53ea\u6f14\u793a\u8fd9\u79cd\u7ed3\u679c\u53ef\u4ee5\u600e\u6837\u51fa\u73b0\uff0c\u4e0d\u628a\u4e00\u4e2a\u4eba\u5de5\u6df7\u5408\u5b9e\u9a8c\u8f6c\u5316\u4e3a\u6240\u6709\u771f\u5b9e\u6570\u636e\u901a\u7528\u7684\u8d28\u91cf\u5224\u5b9a\u95e8\u69db\u3002<\/p>\n
\u8bbe\u8ba1\u4e00\u81f4\u7387\u4e3a\u767e\u5206\u4e4b\u4e5d\u5341\u4e5d\uff0c\u5b9e\u6d4b\u4e3a\u4ec0\u4e48\u662f 98.92%<\/h2>\n
\u8bbe\u8ba1\u7a81\u53d8\u7ec4\u7684\u4fee\u6539\u4f4d\u7f6e\u662f\u660e\u786e\u8bb0\u5f55\u7684\uff0c\u56e0\u6b64\u80fd\u591f\u72ec\u7acb\u8ba1\u7b97\u5b8c\u6574\u5e8f\u5217\u7684\u9010\u4f4d\u4e00\u81f4\u7387\u3002\u5b9e\u9645 skani \u8fd4\u56de\u7684 ANI \u4e3a 98.92%\uff0c\u4e24\u8fb9\u5bf9\u9f50\u6bd4\u4f8b\u5747\u4e3a 99.83%\u3002<\/p>\n
\u4e24\u79cd\u6570\u5b57\u7684\u8ba1\u7b97\u65b9\u5f0f\u4e0d\u540c\u3002\u524d\u8005\u76f4\u63a5\u6839\u636e\u5df2\u77e5\u4fee\u6539\u4f4d\u7f6e\u7edf\u8ba1\u5168\u90e8\u5bf9\u5e94\u78b1\u57fa\uff0c\u540e\u8005\u7531\u8f6f\u4ef6\u7684\u8fd1\u4f3c\u6bd4\u8f83\u8fc7\u7a0b\u4f30\u8ba1\u3002\u5b83\u4eec\u5728\u672c\u4f8b\u4e2d\u63a5\u8fd1\uff0c\u5374\u6ca1\u6709\u9010\u5c0f\u6570\u4f4d\u76f8\u7b49\u3002<\/p>\n
\u56e0\u6b64\uff0c\u4e0d\u80fd\u628a\u8fd9\u7ec4\u7ed3\u679c\u6539\u5199\u6210\u201c\u7cbe\u786e\u68c0\u51fa\u4e86\u5168\u90e8\u7a81\u53d8\u201d\uff0c\u4e5f\u4e0d\u5e94\u628a\u4e24\u8005\u5dee\u503c\u5f53\u6210\u8f6f\u4ef6\u5bf9\u6240\u6709\u6570\u636e\u7684\u56fa\u5b9a\u8bef\u5dee\u3002\u771f\u5b9e\u7ec4\u88c5\u8fd8\u53ef\u80fd\u6d89\u53ca\u91cd\u590d\u3001\u7247\u6bb5\u7f3a\u5931\u3001\u7ed3\u6784\u53d8\u5316\u548c\u4e0d\u5747\u5300\u76f8\u4f3c\u6027\uff0c\u672c\u6b21\u5355\u4e00\u8bbe\u8ba1\u4e0d\u8db3\u4ee5\u8986\u76d6\u8fd9\u4e9b\u60c5\u51b5\u3002<\/p>\n
\u5982\u679c\u7814\u7a76\u7ed3\u8bba\u4f9d\u8d56\u5177\u4f53\u4f4d\u7f6e\u6216\u7cbe\u786e\u7a81\u53d8\u6570\u91cf\uff0c\u5e94\u4fdd\u7559\u76f8\u5e94\u7684\u9010\u4f4d\u7f6e\u8bc1\u636e\u3002skani \u53ef\u4ee5\u5e2e\u52a9\u6311\u9009\u8f83\u63a5\u8fd1\u7684\u53c2\u8003\u548c\u5019\u9009\u6bd4\u8f83\u5bf9\u8c61\uff0c\u4f46\u5176\u6c47\u603b\u8868\u4e0d\u63d0\u4f9b\u5b8c\u6574\u7684\u7a81\u53d8\u6e05\u5355\u3002<\/p>\n
min-af \u5e76\u4e0d\u662f\u8981\u6c42\u53cc\u65b9\u90fd\u8fbe\u5230\u540c\u4e00\u6bd4\u4f8b<\/h2>\n
\u5341\u4e07\u78b1\u57fa\u7247\u6bb5\u4e0e\u53c2\u8003\u7684\u6bd4\u8f83\u4e2d\uff0cANI \u4e3a\u767e\u5206\u4e4b\u767e\uff0c\u67e5\u8be2\u5bf9\u9f50\u6bd4\u4f8b\u4e3a\u767e\u5206\u4e4b\u767e\uff0c\u800c\u53c2\u8003\u6bd4\u4f8b\u53ea\u6709\u7ea6\u767e\u5206\u4e4b\u4e8c\u70b9\u4e00\u4e94\u3002\u968f\u540e\u6211\u4eec\u628a\u6700\u5c0f\u5bf9\u9f50\u6bd4\u4f8b\u8bbe\u4e3a\u4e94\u5341\uff0c\u518d\u6b21\u6267\u884c\u6bd4\u8f83\u3002<\/p>\n
.\/skani dist \\\r\n  -q short.fna -r full.fna \\\r\n  --min-af 50 -o short_min50.tsv \\\r\n  -t 2 --detailed\r\n<\/code><\/pre>\n
\u7ed3\u679c\u4ecd\u7136\u4fdd\u7559\u3002\u539f\u56e0\u53ef\u4ee5\u76f4\u63a5\u4ece\u672c\u6b21\u547d\u4ee4\u5e2e\u52a9\u548c\u5b9e\u9645\u8f93\u51fa\u5bf9\u5e94\u8d77\u6765\uff0c\u8be5\u9009\u9879\u8981\u6c42\u81f3\u5c11\u4e00\u65b9\u8fbe\u5230\u6307\u5b9a\u6761\u4ef6\uff0c\u67e5\u8be2\u8fd9\u4e00\u65b9\u5df2\u7ecf\u6ee1\u8db3\u3002\u5b83\u5e76\u4e0d\u7b49\u4ef7\u4e8e\u8981\u6c42\u53c2\u8003\u548c\u67e5\u8be2\u90fd\u81f3\u5c11\u8986\u76d6\u4e00\u534a\u3002<\/p>\n
\u5982\u679c\u5206\u6790\u8005\u5e0c\u671b\u6392\u9664\u4ec5\u5728\u53c2\u8003\u4e2d\u8986\u76d6\u5f88\u5c0f\u533a\u57df\u7684\u547d\u4e2d\uff0c\u5c31\u4e0d\u80fd\u53ea\u770b\u5230\u53c2\u6570\u4e2d\u5199\u4e86\u4e94\u5341\uff0c\u4fbf\u8ba4\u5b9a\u8fd9\u4e2a\u8981\u6c42\u5df2\u7ecf\u5b9e\u73b0\u3002\u9700\u8981\u68c0\u67e5\u8f6f\u4ef6\u5bf9\u8be5\u9009\u9879\u7684\u5177\u4f53\u5b9a\u4e49\uff0c\u4ee5\u53ca\u5b83\u4f7f\u7528\u7684\u662f\u4e00\u4e2a\u65b9\u5411\u8fd8\u662f\u4e24\u4e2a\u65b9\u5411\u3002<\/p>\n
\u6279\u5904\u7406\u811a\u672c\u4e2d\u6700\u597d\u660e\u786e\u8bb0\u5f55\u9009\u9879\u540d\u79f0\uff0c\u800c\u4e0d\u662f\u53ea\u5728\u62a5\u544a\u4e2d\u5199\u201c\u8986\u76d6\u8fc7\u6ee4\u4e94\u5341\u201d\u3002\u540c\u4e00\u4e2a\u6570\u5b57\u653e\u5728\u4e0d\u540c\u9009\u9879\u540e\u9762\uff0c\u53ef\u80fd\u8868\u8fbe\u4e0d\u540c\u63a5\u53d7\u89c4\u5219\u3002<\/p>\n
\u8981\u6c42\u53cc\u65b9\u90fd\u8fbe\u5230\u6761\u4ef6\uff0c\u8f93\u51fa\u4f1a\u600e\u6837\u53d8\u5316<\/h2>\n
\u4e0b\u4e00\u7ec4\u6539\u7528\u53cc\u65b9\u6700\u5c0f\u5bf9\u9f50\u6bd4\u4f8b\u9009\u9879\uff0c\u4ecd\u8bbe\u4e3a\u4e94\u5341\u3002\u5b9e\u9645\u5341\u4e07\u78b1\u57fa\u7247\u6bb5\u7684\u6bd4\u8f83\u6ca1\u6709\u6570\u636e\u884c\uff0c\u6587\u4ef6\u53ea\u4fdd\u7559\u5217\u6807\u9898\u3002\u5bf9\u4e00\u767e\u4e07\u78b1\u57fa\u7247\u6bb5\u91c7\u7528\u76f8\u540c\u6761\u4ef6\uff0c\u7ed3\u679c\u4e5f\u6ca1\u6709\u6570\u636e\u884c\u3002<\/p>\n
.\/skani dist \\\r\n  -q short.fna -r full.fna \\\r\n  --both-min-af 50 -o short_both50.tsv \\\r\n  -t 2 --detailed\r\n<\/code><\/pre>\n
\u4e24\u6b21\u547d\u4ee4\u90fd\u6b63\u5e38\u9000\u51fa\u3002\u8fd9\u4e0e\u4efb\u52a1\u5931\u8d25\u6216\u6d4b\u5f97 ANI \u4e3a\u96f6\u4e0d\u540c\uff0c\u5019\u9009\u6ca1\u6709\u6ee1\u8db3\u672c\u6b21\u62a5\u544a\u6761\u4ef6\uff0c\u56e0\u6b64\u6ca1\u6709\u8f93\u51fa\u5bf9\u5e94\u6bd4\u8f83\u7ed3\u679c\u3002<\/p>\n
\u5b9e\u9645\u6c47\u603b\u65f6\uff0c\u4e0d\u80fd\u628a\u8fd9\u79cd\u7f3a\u5931\u503c\u76f4\u63a5\u586b\u6210\u96f6\u3002\u586b\u96f6\u4f1a\u628a\u201c\u672a\u901a\u8fc7\u8986\u76d6\u8981\u6c42\u201d\u53d8\u6210\u201c\u5e8f\u5217\u4e00\u81f4\u7a0b\u5ea6\u4e3a\u96f6\u201d\uff0c\u6539\u53d8\u6570\u636e\u542b\u4e49\u3002\u5efa\u8bae\u8bb0\u5f55\u8fc7\u6ee4\u6761\u4ef6\u548c\u662f\u5426\u4ea7\u751f\u6570\u636e\u884c\uff0c\u5fc5\u8981\u65f6\u4fdd\u7559\u8fc7\u6ee4\u524d\u7684\u6bd4\u8f83\u7ed3\u679c\u7528\u4e8e\u8bca\u65ad\u3002<\/p>\n
\u672c\u6b21\u4e94\u5341\u53ea\u662f\u4e3a\u4e86\u660e\u786e\u5c55\u793a\u4e24\u4e2a\u9009\u9879\u7684\u5dee\u5f02\u3002\u6b63\u5f0f\u5206\u6790\u5e94\u6839\u636e\u5bf9\u8c61\u5b8c\u6574\u5ea6\u548c\u7814\u7a76\u95ee\u9898\u9009\u62e9\u6761\u4ef6\uff0c\u5e76\u8bf4\u660e\u4e3a\u4ec0\u4e48\u4f7f\u7528\u5355\u5411\u6216\u53cc\u5411\u8981\u6c42\uff0c\u4e0d\u5b9c\u628a\u6559\u5b66\u5bf9\u7167\u4e2d\u7684\u6570\u5b57\u76f4\u63a5\u5f53\u4f5c\u9ed8\u8ba4\u8d28\u91cf\u6807\u51c6\u3002<\/p>\n
\u4e00\u4e2a FASTA \u6587\u4ef6\u5728\u672c\u6b21\u5b9e\u9a8c\u4e2d\u4ee3\u8868\u4ec0\u4e48<\/h2>\n
\u672c\u6b21\u4f7f\u7528\u9ed8\u8ba4\u6309\u6587\u4ef6\u6bd4\u8f83\u7684\u65b9\u5f0f\uff0c\u6df7\u5408\u6587\u4ef6\u4e2d\u7684\u4e24\u4e2a\u5e8f\u5217\u8bb0\u5f55\u5171\u540c\u6784\u6210\u4e00\u4e2a\u5f85\u6bd4\u8f83\u5bf9\u8c61\u3002\u8be6\u7ec6\u8f93\u51fa\u4e5f\u8bb0\u5f55\u4e86\u67e5\u8be2\u5305\u542b\u4e24\u4e2a\u7247\u6bb5\uff0c\u800c\u4e0d\u662f\u628a\u5b83\u4eec\u81ea\u52a8\u62c6\u6210\u4e24\u4efd\u72ec\u7acb\u57fa\u56e0\u7ec4\u3002<\/p>\n
\u56e0\u6b64\uff0c\u6279\u91cf\u6bd4\u8f83 MAG \u65f6\uff0c\u901a\u5e38\u9700\u8981\u6e05\u695a\u7ef4\u62a4\u4e00\u4e2a\u6587\u4ef6\u5bf9\u5e94\u54ea\u4e2a\u7ec4\u88c5\u3002\u82e5\u628a\u591a\u4e2a\u4e0d\u540c\u57fa\u56e0\u7ec4\u968f\u610f\u5408\u5e76\u5230\u4e00\u4e2a\u6587\u4ef6\uff0c\u518d\u6309\u4e00\u4e2a\u57fa\u56e0\u7ec4\u89e3\u91ca\u6c47\u603b\u6307\u6807\uff0c\u95ee\u9898\u53ef\u80fd\u6765\u81ea\u8f93\u5165\u7ec4\u7ec7\uff0c\u800c\u4e0d\u5728\u6bd4\u8f83\u7b97\u6cd5\u672c\u8eab\u3002<\/p>\n
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