{"id":72833,"date":"2026-11-15T11:33:31","date_gmt":"2026-11-15T03:33:31","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72833"},"modified":"2026-11-15T11:33:31","modified_gmt":"2026-11-15T03:33:31","slug":"prokka-fast-mode-locus-tags-trusted-protein-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/prokka-fast-mode-locus-tags-trusted-protein-practice\/","title":{"rendered":"Prokka \u6ce8\u91ca\u7684\u57fa\u56e0\u6570\u4e00\u6837\uff0c\u529f\u80fd\u540d\u79f0\u4e3a\u4ec0\u4e48\u4e0d\u540c\uff1f\u56db\u7ec4\u5b9e\u8dd1\u5206\u6e05\u5e8f\u5217\u3001\u7f16\u53f7\u4e0e\u547d\u540d\u4f9d\u636e"},"content":{"rendered":"
\n

\u6574\u7406\u4e24\u6b21\u7ec6\u83cc\u6ce8\u91ca\u7ed3\u679c\u65f6\uff0c\u5982\u679c\u57fa\u56e0\u603b\u6570\u76f8\u540c\uff0c\u5374\u53d1\u73b0\u5f88\u591a\u529f\u80fd\u540d\u79f0\u53d8\u4e86\uff0c\u4e0d\u80fd\u76f4\u63a5\u4e0b\u7ed3\u8bba\u8bf4\u8f6f\u4ef6\u53d1\u73b0\u4e86\u4e0d\u540c\u7684\u57fa\u56e0\u3002\u4f4d\u7f6e\u9884\u6d4b\u3001\u86cb\u767d\u7ffb\u8bd1\u3001\u529f\u80fd\u547d\u540d\u548c\u6761\u76ee\u7f16\u53f7\u5c5e\u4e8e\u4e0d\u540c\u5c42\u6b21\uff0c\u5fc5\u987b\u5206\u522b\u6838\u5bf9\uff0c\u624d\u80fd\u77e5\u9053\u53d8\u5316\u53d1\u751f\u5728\u54ea\u4e00\u6b65\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u5b89\u88c5 Prokka 1.15.6\uff0c\u4ece\u516c\u5f00\u5927\u80a0\u6746\u83cc\u53c2\u8003\u4e2d\u53d6\u51fa\u540c\u4e00\u6bb5\u4e8c\u5341\u4e07\u78b1\u57fa\u5e8f\u5217\uff0c\u8fd0\u884c\u666e\u901a\u6a21\u5f0f\u3001\u5feb\u901f\u6a21\u5f0f\u3001\u6539\u53d8\u4f4d\u70b9\u6807\u7b7e\uff0c\u4ee5\u53ca\u52a0\u5165\u6307\u5b9a\u86cb\u767d\u56db\u7ec4\u5bf9\u7167\u3002\u6240\u6709\u8f93\u5165\u6838\u9178\u5b8c\u5168\u76f8\u540c\uff0c\u968f\u540e\u6bd4\u8f83\u6bcf\u4e2a\u7279\u5f81\u7684\u5750\u6807\u3001\u86cb\u767d\u5e8f\u5217\u6821\u9a8c\u503c\u548c\u529f\u80fd\u540d\u79f0\uff0c\u8ba9\u5dee\u5f02\u80fd\u591f\u8ffd\u6eaf\u5230\u5177\u4f53\u53c2\u6570\u3002<\/p>\n

\"\u57fa\u56e0\u6570\u76f8\u540c\uff0c\u540d\u79f0\u4ecd\u4f1a\u6539\u53d8\u3002\u56db\u7ec4\u5b9e\u8dd1\u5206\u6e05\u5e8f\u5217\u3001\u7f16\u53f7\u548c\u547d\u540d\u4f9d\u636e\u3002\u666e\u901a\u4e0e\u5feb\u901f\u6a21\u5f0f\uff0cCDS
\u56db\u7ec4\u5b9e\u8dd1\u5206\u6e05\u5e8f\u5217\u3001\u7f16\u53f7\u548c\u547d\u540d\u4f9d\u636e<\/figcaption><\/figure>\n

\u4f7f\u7528\u524d\u5148\u77e5\u9053\u8fd9\u4e2a\u9879\u76ee\u76ee\u524d\u7684\u5b9a\u4f4d<\/h2>\n

Prokka \u4ecd\u5e38\u89c1\u4e8e\u5df2\u6709\u7ec6\u83cc\u5206\u6790\u6d41\u7a0b\u548c\u5386\u53f2\u9879\u76ee\u4e2d\u3002\u6838\u5bf9\u672c\u6b21\u8d44\u6599\u65f6\uff0c\u9879\u76ee\u4f5c\u8005\u5df2\u5728\u8bf4\u660e\u4e2d\u5efa\u8bae\u540e\u7eed\u6d41\u7a0b\u8003\u8651 Bakta\uff0c\u7248\u672c\u53d1\u5e03\u8bf4\u660e\u4e5f\u660e\u786e\u4e86\u7ef4\u62a4\u65b9\u5411\u3002\u56e0\u6b64\uff0c\u672c\u6587\u66f4\u9002\u5408\u9700\u8981\u590d\u73b0\u5df2\u6709\u7ed3\u679c\u3001\u68c0\u67e5\u6ce8\u91ca\u6587\u4ef6\u6216\u7406\u89e3\u53c2\u6570\u884c\u4e3a\u7684\u8bfb\u8005\uff0c\u4e0d\u628a\u5386\u53f2\u4f7f\u7528\u5e7f\u6cdb\u7b49\u540c\u4e8e\u4ecd\u5728\u79ef\u6781\u6269\u5c55\u529f\u80fd\u3002Prokka \u9879\u76ee\u8bf4\u660e<\/a><\/p>\n

\u65e7\u9879\u76ee\u5e76\u4e0d\u9700\u8981\u56e0\u4e3a\u6709\u4e86\u65b0\u5de5\u5177\uff0c\u5c31\u7acb\u5373\u628a\u6240\u6709\u6837\u672c\u91cd\u65b0\u6ce8\u91ca\u3002\u5982\u679c\u7814\u7a76\u8981\u4e0e\u65e2\u6709\u7ed3\u679c\u6bd4\u8f83\uff0c\u9996\u5148\u5e94\u786e\u8ba4\u65e7\u8f6f\u4ef6\u3001\u6570\u636e\u5e93\u548c\u53c2\u6570\u662f\u5426\u80fd\u591f\u91cd\u73b0\uff0c\u518d\u5355\u72ec\u8bc4\u4f30\u66ff\u6362\u5de5\u5177\u540e\u4ea7\u751f\u7684\u53d8\u5316\u3002\u5426\u5219\uff0c\u7248\u672c\u66f4\u6362\u4e0e\u6837\u672c\u5dee\u5f02\u6df7\u5728\u4e00\u8d77\uff0c\u5f88\u96be\u89e3\u91ca\u65b0\u589e\u6216\u51cf\u5c11\u7684\u57fa\u56e0\u3002<\/p>\n

\u5b89\u88c5\u540e\u4e3a\u4ec0\u4e48\u8fd8\u8981\u8bb0\u5f55\u6570\u636e\u5e93<\/h2>\n

\u672c\u6b21\u4f7f\u7528 BioContainers \u7684\u56fa\u5b9a\u7248\u672c\u955c\u50cf\uff0c\u5b9e\u9645\u7248\u672c\u8f93\u51fa\u4e3a 1.15.6\u3002\u955c\u50cf\u6458\u8981\u3001\u6570\u636e\u5e93\u5217\u8868\u4ee5\u53ca\u5b9e\u8dd1\u811a\u672c\u4e00\u5e76\u4fdd\u5b58\u5728\u590d\u73b0\u6750\u6599\u4e2d\uff0c\u907f\u514d\u53ea\u4fdd\u7559\u4e00\u4e2a\u5bb9\u6613\u53d8\u5316\u7684\u955c\u50cf\u6807\u7b7e\u3002<\/p>\n

PROKKA_IMAGE='quay.io\/biocontainers\/prokka:1.15.6--pl5321hcbfc158_1'\r\ndocker pull "$PROKKA_IMAGE"\r\ndocker run --rm "$PROKKA_IMAGE" prokka --version\r\ndocker run --rm "$PROKKA_IMAGE" prokka --listdb\r\n<\/code><\/pre>\n
\u8f6f\u4ef6\u7248\u672c\u544a\u8bc9\u6211\u4eec\u8c03\u7528\u7684\u662f\u54ea\u4e00\u5957\u7a0b\u5e8f\u903b\u8f91\uff0c\u6570\u636e\u5e93\u5217\u8868\u548c\u73af\u5883\u6458\u8981\u5e2e\u52a9\u786e\u8ba4\u5b83\u5b9e\u9645\u80fd\u591f\u641c\u7d22\u54ea\u4e9b\u53c2\u8003\u3002\u529f\u80fd\u540d\u79f0\u4f9d\u8d56\u8fd9\u4e9b\u53c2\u8003\uff0c\u5355\u72ec\u8bb0\u5f55\u8f6f\u4ef6\u7248\u672c\u4e0d\u8db3\u4ee5\u89e3\u91ca\u547d\u540d\u5dee\u5f02\u3002\u5bf9\u6b63\u5f0f\u7814\u7a76\uff0c\u8fd8\u5e94\u4fdd\u7559\u955c\u50cf\u6458\u8981\u6216\u660e\u786e\u7684\u6570\u636e\u5e93\u6587\u4ef6\u8bb0\u5f55\u3002<\/p>\n
\u5b89\u88c5\u6210\u529f\u4e5f\u4e0d\u4ee3\u8868\u6240\u6709\u7814\u7a76\u5bf9\u8c61\u90fd\u9002\u7528\u3002\u9700\u8981\u5148\u786e\u8ba4\u8f93\u5165\u662f\u9002\u5408\u8be5\u6d41\u7a0b\u7684\u539f\u6838\u7ec4\u88c5\uff0c\u5e76\u660e\u786e\u9057\u4f20\u5bc6\u7801\u8868\u4e0e\u5206\u6790\u76ee\u7684\u3002\u672c\u6587\u56fa\u5b9a\u7ec6\u83cc\u6a21\u5f0f\uff0c\u4e0d\u628a\u8fd9\u7ec4\u5b9e\u9a8c\u63a8\u5e7f\u5230\u771f\u6838\u57fa\u56e0\u7ec4\uff0c\u4e5f\u6ca1\u6709\u6bd4\u8f83\u4e0d\u540c\u9057\u4f20\u5bc6\u7801\u8868\u5e26\u6765\u7684\u7ffb\u8bd1\u53d8\u5316\u30021.15.6 \u7248\u672c\u8bf4\u660e<\/a><\/p>\n
\u540c\u4e00\u6bb5\u8f93\u5165\u5982\u4f55\u505a\u5230\u53ef\u6838\u5bf9<\/h2>\n
\u516c\u5f00\u53c2\u8003\u4e3a\u5927\u80a0\u6746\u83cc K-12 MG1655 \u7684 NC_000913.3\uff0c\u5b8c\u6574\u5e8f\u5217\u957f\u5ea6\u4e3a\u56db\u767e\u516d\u5341\u56db\u4e07\u4e00\u5343\u516d\u767e\u4e94\u5341\u4e8c\u78b1\u57fa\u3002\u9a71\u52a8\u9996\u5148\u68c0\u67e5\u5b8c\u6574\u5e8f\u5217\u957f\u5ea6\u4e0e\u6821\u9a8c\u503c\uff0c\u518d\u53d6\u4e00\u5230\u4e8c\u5341\u4e07\u7684\u8fde\u7eed\u533a\u95f4\u4f5c\u4e3a\u672c\u6b21\u8f93\u5165\uff0c\u5199\u6210\u53ea\u6709\u4e00\u6761\u8bb0\u5f55\u7684 FASTA\u3002NCBI \u53c2\u8003\u5e8f\u5217<\/a><\/p>\n
\u8fd9\u91cc\u5206\u6790\u7684\u662f\u53c2\u8003\u4e2d\u7684\u4e00\u4e2a\u7247\u6bb5\uff0c\u4e0d\u80fd\u628a\u5b83\u7684\u57fa\u56e0\u6570\u91cf\u62a5\u544a\u4e3a\u5b8c\u6574\u83cc\u682a\u57fa\u56e0\u7ec4\u7684\u6ce8\u91ca\u6570\u91cf\u3002\u622a\u53d6\u8fb9\u754c\u53ef\u80fd\u5207\u65ad\u539f\u6709\u57fa\u56e0\uff0c\u57fa\u56e0\u9884\u6d4b\u6a21\u578b\u4e5f\u4f1a\u6839\u636e\u672c\u6b21\u8f93\u5165\u8bad\u7ec3\uff0c\u56e0\u6b64\u6240\u5f97\u7ed3\u679c\u53ea\u4ee3\u8868\u8fd9\u6bb5\u8f93\u5165\u548c\u5bf9\u5e94\u53c2\u6570\u3002<\/p>\n
\u6821\u9a8c\u65f6\u4f7f\u7528\u53bb\u6389\u6807\u9898\u3001\u8fde\u63a5\u6240\u6709\u5e8f\u5217\u884c\u5e76\u8f6c\u4e3a\u5927\u5199\u540e\u7684\u6838\u9178\u5185\u5bb9\u3002\u8fd9\u6837\u53ef\u4ee5\u533a\u5206\u6362\u884c\u6216\u6807\u9898\u6539\u53d8\u4e0e\u78b1\u57fa\u672c\u8eab\u6539\u53d8\u3002\u56db\u7ec4\u5b9e\u9a8c\u5171\u7528\u540c\u4e00\u4efd\u7247\u6bb5\u6587\u4ef6\uff0c\u5e76\u8fdb\u4e00\u6b65\u786e\u8ba4\u8f93\u51fa\u6838\u9178\u4ecd\u4e0e\u8be5\u7247\u6bb5\u4e00\u81f4\uff0c\u9632\u6b62\u6bd4\u8f83\u8fc7\u7a0b\u4e2d\u8bef\u7528\u4e86\u4e0d\u540c\u7ec4\u88c5\u7248\u672c\u3002<\/p>\n
\u666e\u901a\u6a21\u5f0f\u5148\u5efa\u7acb\u53ef\u4ee5\u9010\u9879\u5bf9\u7167\u7684\u57fa\u7ebf<\/h2>\n
\u57fa\u7ebf\u660e\u786e\u8bbe\u7f6e\u8f93\u51fa\u76ee\u5f55\u3001\u6587\u4ef6\u524d\u7f00\u3001\u4f4d\u70b9\u6807\u7b7e\u548c\u7ebf\u7a0b\u6570\u3002\u6587\u4ef6\u524d\u7f00\u51b3\u5b9a\u4e00\u7ec4\u7ed3\u679c\u53eb\u4ec0\u4e48\uff0c\u4f4d\u70b9\u6807\u7b7e\u7528\u4e8e\u8f93\u51fa\u7279\u5f81\u7f16\u53f7\uff0c\u4e8c\u8005\u5e76\u4e0d\u66ff\u4ee3\u5e8f\u5217\u8eab\u4efd\u3002<\/p>\n
prokka --cpus 2 --kingdom Bacteria \\\r\n  --outdir baseline --prefix sample \\\r\n  --locustag BASE segment.fna\r\n<\/code><\/pre>\n
\u672c\u6b21\u57fa\u7ebf\u5b9e\u9645\u5f97\u5230 169 \u6761 CDS \u548c 169 \u6761\u86cb\u767d\u8bb0\u5f55\uff0c\u5176\u4e2d 16 \u6761 CDS \u88ab\u547d\u540d\u4e3a\u5047\u5b9a\u86cb\u767d\u3002\u8fd9\u4e2a\u7edf\u8ba1\u53ea\u63cf\u8ff0\u8f6f\u4ef6\u5f53\u524d\u7ed9\u51fa\u7684\u7ed3\u679c\uff0c\u4e0d\u8868\u793a\u5176\u4f59\u6761\u76ee\u5df2\u7ecf\u5168\u90e8\u7ecf\u8fc7\u5b9e\u9a8c\u9a8c\u8bc1\u3002<\/p>\n
\u68c0\u67e5\u65f6\u4e0d\u80fd\u53ea\u8bfb\u53d6\u6700\u540e\u4e00\u884c\u7edf\u8ba1\u3002\u6211\u4eec\u540c\u65f6\u89e3\u6790\u4e3b\u6ce8\u91ca\u6587\u4ef6\u4e0e\u86cb\u767d\u6587\u4ef6\uff0c\u4e3a\u6bcf\u6761\u86cb\u767d\u8ba1\u7b97\u5e8f\u5217\u6821\u9a8c\u503c\uff0c\u5e76\u4ee5\u5e8f\u5217\u7f16\u53f7\u3001\u8d77\u6b62\u4f4d\u7f6e\u3001\u65b9\u5411\u548c\u7279\u5f81\u7c7b\u578b\u5171\u540c\u5b9a\u4f4d\u6761\u76ee\u3002\u8fd9\u6837\u540e\u7eed\u5373\u4f7f\u540d\u79f0\u6216\u6807\u7b7e\u53d1\u751f\u53d8\u5316\uff0c\u4ecd\u7136\u80fd\u591f\u627e\u5230\u5bf9\u5e94\u5bf9\u8c61\u3002<\/p>\n
\u51e0\u79cd\u8f93\u51fa\u6587\u4ef6\u5206\u522b\u56de\u7b54\u4ec0\u4e48\u95ee\u9898<\/h2>\n
\u4e3b GFF \u6587\u4ef6\u5305\u542b\u7279\u5f81\u5750\u6807\u548c\u5c5e\u6027\uff0c\u5e76\u5e26\u6709\u8f93\u5165\u5e8f\u5217\u90e8\u5206\u3002\u67e5\u770b\u5b83\u53ef\u4ee5\u786e\u8ba4\u67d0\u4e2a\u6761\u76ee\u5728\u54ea\u6761\u5e8f\u5217\u3001\u54ea\u4e2a\u533a\u95f4\u548c\u54ea\u6761\u94fe\u4e0a\u3002\u86cb\u767d FASTA \u5219\u9002\u5408\u68c0\u67e5\u7ffb\u8bd1\u4ea7\u7269\uff0c\u6216\u4f5c\u4e3a\u540e\u7eed\u86cb\u767d\u641c\u7d22\u548c\u805a\u7c7b\u8f93\u5165\u3002<\/p>\n
\u6838\u9178\u7279\u5f81\u6587\u4ef6\u4e0e\u86cb\u767d\u6587\u4ef6\u4e0d\u80fd\u76f4\u63a5\u4e92\u6362\u3002\u524d\u8005\u8fd8\u53ef\u80fd\u5305\u542b RNA \u7b49\u975e\u86cb\u767d\u7279\u5f81\uff0c\u56e0\u6b64\u4e24\u4efd\u6587\u4ef6\u7684\u8bb0\u5f55\u6570\u4e0d\u4e00\u5b9a\u76f8\u7b49\u3002\u672c\u6b21\u6838\u9178\u7279\u5f81\u6587\u4ef6\u4e0e\u86cb\u767d\u6587\u4ef6\u6070\u597d\u90fd\u662f\u4e00\u767e\u516d\u5341\u4e5d\u6761\uff0c\u56e0\u4e3a\u5f53\u524d\u8fd9\u6bb5\u8f93\u5165\u7684\u8f93\u51fa\u7279\u5f81\u5747\u4e3a CDS\u3002\u8fd9\u79cd\u76f8\u7b49\u5173\u7cfb\u4e0d\u80fd\u63a8\u5e7f\u5230\u5305\u542b RNA \u6ce8\u91ca\u7684\u5176\u4ed6\u6570\u636e\u3002<\/p>\n
\u53e6\u5916\uff0c\u529f\u80fd\u540d\u79f0\u8868\u65b9\u4fbf\u5feb\u901f\u7b5b\u9009\uff0c\u4f46\u5b83\u4e0d\u80fd\u66ff\u4ee3\u5b8c\u6574\u5750\u6807\u548c\u539f\u59cb\u5e8f\u5217\u3002\u82e5\u53ea\u628a\u540d\u79f0\u8868\u5bfc\u51fa\u5230\u7535\u5b50\u8868\u683c\uff0c\u65e5\u540e\u5f88\u96be\u6838\u5bf9\u67d0\u4e2a\u540d\u79f0\u53d8\u5316\u662f\u5426\u5bf9\u5e94\u540c\u4e00\u4e2a\u86cb\u767d\u3002\u5efa\u8bae\u81f3\u5c11\u5171\u540c\u4fdd\u7559\u4e3b\u6ce8\u91ca\u3001\u6838\u9178\u3001\u86cb\u767d\u3001\u7edf\u8ba1\u53ca\u65e5\u5fd7\u6587\u4ef6\u3002<\/p>\n
\u5feb\u901f\u6a21\u5f0f\u6539\u53d8\u4e86\u54ea\u4e9b\u7ed3\u679c<\/h2>\n
\u7b2c\u4e8c\u7ec4\u4ec5\u589e\u52a0\u5feb\u901f\u6a21\u5f0f\u9009\u9879\uff0c\u5176\u4f59\u8f93\u5165\u4e0e\u4e3b\u8981\u53c2\u6570\u4fdd\u6301\u4e00\u81f4\u3002\u7a0b\u5e8f\u5b9e\u9645\u8fd0\u884c\u540e\uff0c\u518d\u628a\u5b83\u4e0e\u666e\u901a\u6a21\u5f0f\u9010\u6761\u5339\u914d\u3002<\/p>\n
prokka --cpus 2 --kingdom Bacteria \\\r\n  --outdir fast --prefix sample \\\r\n  --locustag BASE --fast segment.fna\r\n<\/code><\/pre>\n
\u672c\u6b21\u4e24\u7ec4\u7684\u7279\u5f81\u5750\u6807\u96c6\u5408\u4e00\u81f4\uff0c\u86cb\u767d\u5e8f\u5217\u53ca\u5176\u91cd\u590d\u6b21\u6570\u4e5f\u4e00\u81f4\uff0c\u4f46\u5feb\u901f\u6a21\u5f0f\u4e2d\u5047\u5b9a\u86cb\u767d\u6761\u76ee\u589e\u81f3 17 \u6761\u3002\u53ef\u89c1\uff0c\u7ed3\u6784\u9884\u6d4b\u7ed3\u679c\u76f8\u540c\uff0c\u5e76\u4e0d\u8981\u6c42\u529f\u80fd\u540d\u79f0\u4e5f\u76f8\u540c\u3002\u5b9e\u9645\u65e5\u5fd7\u663e\u793a\uff0c\u5feb\u901f\u6a21\u5f0f\u4ecd\u8fd0\u884c\u57fa\u7840 BLAST \u641c\u7d22\uff0c\u8df3\u8fc7\u7684\u662f\u975e BLAST \u641c\u7d22\uff0c\u5176\u4e2d\u5305\u62ec\u672c\u6b21\u7684 HAMAP \u9690\u9a6c\u5c14\u53ef\u592b\u6a21\u578b\u641c\u7d22\u3002\u56e0\u6b64\uff0c\u4e0d\u80fd\u628a\u8fd9\u4e2a\u9009\u9879\u89e3\u91ca\u4e3a\u5b8c\u5168\u4e0d\u505a\u529f\u80fd\u641c\u7d22\u3002<\/p>\n
\u8fd9\u79cd\u5bf9\u7167\u9002\u5408\u68c0\u67e5\u6d41\u7a0b\u662f\u5426\u80fd\u591f\u4ece\u6838\u9178\u4ea7\u751f\u9884\u6d4b\u7f16\u7801\u533a\u548c\u86cb\u767d\u5e8f\u5217\u3002\u4f46\u5982\u679c\u7814\u7a76\u91cd\u70b9\u662f\u529f\u80fd\u6784\u6210\uff0c\u4e0d\u80fd\u628a\u5feb\u901f\u6a21\u5f0f\u4e0e\u5b8c\u6574\u641c\u7d22\u6a21\u5f0f\u7684\u5047\u5b9a\u86cb\u767d\u6bd4\u4f8b\u76f4\u63a5\u5f53\u6210\u6837\u672c\u4e4b\u95f4\u7684\u751f\u7269\u5b66\u5dee\u5f02\u3002<\/p>\n
\u8fd8\u9700\u8981\u907f\u514d\u53e6\u4e00\u79cd\u8bef\u8bfb\u3002\u5047\u5b9a\u86cb\u767d\u5e76\u4e0d\u7b49\u4e8e\u6ca1\u6709\u9884\u6d4b\u5230\u86cb\u767d\uff0c\u4e5f\u4e0d\u7b49\u4e8e\u8be5\u4f4d\u7f6e\u6ca1\u6709\u57fa\u56e0\u3002\u5b83\u8bf4\u660e\u5f53\u524d\u7ed3\u679c\u6ca1\u6709\u7ed9\u51fa\u8db3\u591f\u5177\u4f53\u7684\u529f\u80fd\u547d\u540d\uff0c\u7ed3\u6784\u9884\u6d4b\u4e0e\u529f\u80fd\u89e3\u91ca\u4ecd\u7136\u662f\u4e24\u4ef6\u9700\u8981\u5206\u522b\u6838\u5bf9\u7684\u4e8b\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
\u8fd0\u884c\u6761\u4ef6<\/th>\nCDS \u6570<\/th>\n\u5047\u5b9a\u86cb\u767d\u6570<\/th>\n\u4e0e\u57fa\u7ebf\u86cb\u767d\u5e8f\u5217\u6bd4\u8f83<\/th>\n<\/tr>\n<\/thead>\n
\u666e\u901a\u6a21\u5f0f<\/td>\n169<\/td>\n16<\/td>\n\u57fa\u7ebf<\/td>\n<\/tr>\n
\u5feb\u901f\u6a21\u5f0f<\/td>\n169<\/td>\n17<\/td>\n\u5b8c\u5168\u4e00\u81f4<\/td>\n<\/tr>\n
\u6539\u53d8\u4f4d\u70b9\u6807\u7b7e<\/td>\n169<\/td>\n16<\/td>\n\u5b8c\u5168\u4e00\u81f4<\/td>\n<\/tr>\n
\u52a0\u5165\u6307\u5b9a\u86cb\u767d<\/td>\n169<\/td>\n16<\/td>\n\u5b8c\u5168\u4e00\u81f4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u54ea\u4e00\u6761\u529f\u80fd\u540d\u53d8\u4e86\uff0c\u65e5\u5fd7\u80fd\u5426\u89e3\u91ca<\/h2>\n
\u672c\u6b21\u666e\u901a\u6a21\u5f0f\u4e0e\u5feb\u901f\u6a21\u5f0f\u53ea\u6709\u4e00\u6761\u4ea7\u7269\u540d\u79f0\u4e0d\u540c\uff0c\u5bf9\u5e94\u7247\u6bb5\u56db\u4e07\u56db\u5343\u4e00\u767e\u516b\u5341\u5230\u56db\u4e07\u4e94\u5343\u56db\u767e\u516d\u5341\u516d\u7684\u6b63\u94fe CDS\u3002\u666e\u901a\u6a21\u5f0f\u5c06\u5176\u547d\u540d\u4e3a\u786b\u80fa\u7d20\u567b\u5511\u5408\u9176\uff0c\u5feb\u901f\u6a21\u5f0f\u5c06\u5176\u4fdd\u7559\u4e3a\u5047\u5b9a\u86cb\u767d\u3002\u4e24\u6761\u8bb0\u5f55\u7684\u9884\u6d4b\u5750\u6807\u548c\u86cb\u767d\u5e8f\u5217\u5b8c\u5168\u4e00\u81f4\uff0c\u6240\u4ee5\u5dee\u5f02\u53d1\u751f\u5728\u529f\u80fd\u547d\u540d\u5c42\u9762\u3002<\/p>\n
\u6211\u4eec\u4e0d\u4ec5\u67e5\u770b\u4e86\u6700\u7ec8\u8868\u683c\uff0c\u8fd8\u6838\u5bf9\u4e86\u5b9e\u9645\u641c\u7d22\u65e5\u5fd7\u548c\u56fa\u5b9a\u7248\u672c\u6e90\u7801\u3002\u666e\u901a\u6a21\u5f0f\u6267\u884c\u4e86\u57fa\u7840\u86cb\u767d\u641c\u7d22\u4e0e\u540e\u7eed\u6a21\u578b\u641c\u7d22\uff0c\u5feb\u901f\u6a21\u5f0f\u660e\u786e\u8bb0\u5f55\u8df3\u8fc7\u975e BLAST \u6570\u636e\u5e93\u3002\u7a0b\u5e8f\u5e2e\u52a9\u4e5f\u628a\u8be5\u9009\u9879\u63cf\u8ff0\u4e3a\u53ea\u4f7f\u7528\u57fa\u7840 BLASTP \u6570\u636e\u5e93\uff0c\u8fd9\u6bd4\u628a\u5b83\u7b3c\u7edf\u79f0\u4e3a\u201c\u5173\u95ed\u529f\u80fd\u6ce8\u91ca\u201d\u66f4\u51c6\u786e\u3002\u56fa\u5b9a\u7248\u672c\u7684\u53c2\u6570\u4e0e\u641c\u7d22\u5b9e\u73b0<\/a><\/p>\n
\u8fd9\u4e2a\u7ecf\u9a8c\u9002\u7528\u4e8e\u9605\u8bfb\u8bb8\u591a\u5de5\u5177\u6559\u7a0b\u3002\u4e00\u4e2a\u9009\u9879\u540d\u79f0\u53ef\u80fd\u4fdd\u6301\u591a\u5e74\uff0c\u4f46\u5e2e\u52a9\u6587\u5b57\u3001\u6d41\u7a0b\u548c\u9ed8\u8ba4\u6570\u636e\u5e93\u4f1a\u53d8\u5316\u3002\u89e3\u91ca\u5b9e\u8dd1\u7ed3\u679c\u65f6\uff0c\u5e94\u628a\u5f53\u524d\u7248\u672c\u5e2e\u52a9\u3001\u65e5\u5fd7\u548c\u8f93\u51fa\u76f8\u4e92\u6838\u5bf9\uff0c\u800c\u4e0d\u80fd\u53ea\u4f9d\u8d56\u65e7\u6559\u7a0b\u4e2d\u4e00\u53e5\u7b80\u77ed\u8bf4\u660e\u3002<\/p>\n
\u6539\u7f16\u53f7\u4ee5\u540e\uff0c\u600e\u6837\u786e\u8ba4\u4ecd\u662f\u540c\u4e00\u6761\u86cb\u767d<\/h2>\n
\u7b2c\u4e09\u7ec4\u628a\u4f4d\u70b9\u6807\u7b7e\u4ece BASE \u6539\u6210 RENAMED\uff0c\u5176\u4ed6\u6761\u4ef6\u4fdd\u6301\u57fa\u7ebf\u8bbe\u7f6e\u3002\u5b9e\u9645\u6bd4\u8f83\u53d1\u73b0\uff0c169 \u4e2a\u5171\u4eab\u7279\u5f81\u7684\u7f16\u53f7\u6539\u53d8\uff0c\u4f46\u7279\u5f81\u5750\u6807\u548c\u86cb\u767d\u5e8f\u5217\u96c6\u5408\u6ca1\u6709\u53d8\u5316\u3002<\/p>\n
prokka --cpus 2 --kingdom Bacteria \\\r\n  --outdir renamed --prefix sample \\\r\n  --locustag RENAMED segment.fna\r\n<\/code><\/pre>\n
\u56e0\u6b64\uff0c\u4e0d\u80fd\u62ff\u4e24\u4e2a\u4e0d\u540c\u6807\u7b7e\u7684\u540d\u79f0\u8868\u76f4\u63a5\u6309\u7f16\u53f7\u6c42\u4ea4\u96c6\uff0c\u518d\u628a\u6ca1\u6709\u5339\u914d\u4e0a\u7684\u7f16\u53f7\u89e3\u91ca\u4e3a\u65b0\u589e\u6216\u4e22\u5931\u57fa\u56e0\u3002\u7f16\u53f7\u53ef\u4ee5\u5e2e\u52a9\u5b9a\u4f4d\u5f53\u524d\u7ed3\u679c\uff0c\u4f46\u662f\u5426\u5c5e\u4e8e\u540c\u4e00\u4e2a\u751f\u7269\u5e8f\u5217\u5bf9\u8c61\uff0c\u8fd8\u9700\u8981\u7ed3\u5408\u5e8f\u5217\u4e0e\u5750\u6807\u5224\u65ad\u3002<\/p>\n
\u672c\u4f8b\u53ea\u6539\u53d8\u6807\u7b7e\u524d\u7f00\uff0c\u5750\u6807\u6ca1\u6709\u53d8\u5316\uff0c\u6240\u4ee5\u5339\u914d\u6bd4\u8f83\u76f4\u63a5\u3002\u771f\u5b9e\u9879\u76ee\u5982\u679c\u540c\u65f6\u53d1\u751f\u7ec4\u88c5\u4fee\u8ba2\u3001\u5e8f\u5217\u91cd\u6392\u6216\u8fb9\u754c\u6539\u53d8\uff0c\u539f\u5750\u6807\u53ef\u80fd\u4e5f\u4e0d\u80fd\u76f4\u63a5\u4f7f\u7528\uff0c\u9700\u8981\u501f\u52a9\u5e8f\u5217\u6bd4\u5bf9\u5efa\u7acb\u5bf9\u5e94\u5173\u7cfb\u3002\u6821\u9a8c\u503c\u5b8c\u5168\u76f8\u540c\u53ef\u4ee5\u5feb\u901f\u786e\u8ba4\u76f8\u540c\u5e8f\u5217\uff0c\u6821\u9a8c\u503c\u4e0d\u540c\u5219\u53ea\u8bf4\u660e\u5185\u5bb9\u4e0d\u540c\uff0c\u4e0d\u544a\u8bc9\u6211\u4eec\u5dee\u5f02\u6709\u591a\u5927\u3002<\/p>\n
\u8fd8\u6709\u4e00\u79cd\u9700\u8981\u4fdd\u7559\u7684\u60c5\u51b5\u662f\u91cd\u590d\u86cb\u767d\u3002\u82e5\u4e24\u4e2a\u4f4d\u7f6e\u4ea7\u751f\u76f8\u540c\u86cb\u767d\uff0c\u7b80\u5355\u53bb\u91cd\u6210\u4e00\u4e2a\u6821\u9a8c\u503c\u4f1a\u4e22\u6389\u62f7\u8d1d\u4fe1\u606f\u3002\u672c\u6b21\u6bd4\u8f83\u4f7f\u7528\u5305\u542b\u91cd\u590d\u6b21\u6570\u7684\u5e8f\u5217\u96c6\u5408\uff0c\u56e0\u6b64\u4e0d\u4ec5\u68c0\u67e5\u6709\u54ea\u4e9b\u5e8f\u5217\uff0c\u4e5f\u68c0\u67e5\u6bcf\u79cd\u5e8f\u5217\u51fa\u73b0\u51e0\u6b21\u3002<\/p>\n
\u6307\u5b9a\u86cb\u767d\u5982\u4f55\u5f71\u54cd\u529f\u80fd\u547d\u540d<\/h2>\n
\u7b2c\u56db\u7ec4\u4ece\u57fa\u7ebf\u4e2d\u9009\u53d6\u4e00\u6761\u957f\u5ea6\u81f3\u5c11\u4e00\u767e\u4e94\u5341\u4e2a\u6c28\u57fa\u9178\u7684\u86cb\u767d\uff0c\u539f\u6837\u590d\u5236\u5176\u5e8f\u5217\uff0c\u7ed9\u5b83\u4e00\u4e2a\u4e13\u7528\u4e8e\u5bf9\u7167\u7684\u540d\u79f0\u3002\u6211\u4eec\u660e\u786e\u628a\u8fd9\u4e2a\u540d\u79f0\u89c6\u4e3a\u4eba\u4e3a\u6807\u7b7e\uff0c\u4e0d\u628a\u5b83\u5f53\u4f5c\u65b0\u589e\u529f\u80fd\u8bc1\u636e\u3002<\/p>\n
\u672c\u6b21\u9009\u4e2d\u7684\u662f BASE_00001\uff0c\u957f\u5ea6\u4e3a 820 \u4e2a\u6c28\u57fa\u9178\u3002\u9a71\u52a8\u4e3a\u5b83\u8bbe\u7f6e\u6f14\u793a\u7528\u57fa\u56e0\u540d demoX<\/code> \u548c\u4ea7\u7269\u540d\u79f0 Controlled annotation label<\/code>\uff0c\u518d\u901a\u8fc7\u6307\u5b9a\u86cb\u767d\u9009\u9879\u4ea4\u7ed9\u8f6f\u4ef6\u3002<\/p>\n
prokka --cpus 2 --kingdom Bacteria \\\r\n  --outdir trusted --prefix sample \\\r\n  --locustag BASE --proteins trusted.faa segment.fna\r\n<\/code><\/pre>\n
\u5b9e\u9645\u7ed3\u679c\u4e2d\uff0c\u76ee\u6807\u6761\u76ee\u7684\u547d\u540d\u968f\u6307\u5b9a\u53c2\u8003\u53d1\u751f\u53d8\u5316\uff0c\u86cb\u767d\u5e8f\u5217\u5374\u4e0e\u57fa\u7ebf\u4e00\u81f4\u3002\u8fd9\u8bf4\u660e\u53ef\u4fe1\u86cb\u767d\u8f93\u5165\u4f1a\u53c2\u4e0e\u5e76\u5f71\u54cd\u547d\u540d\u51b3\u7b56\u3002\u82e5\u53ef\u4fe1\u5e93\u672c\u8eab\u6807\u9519\u4e86\uff0c\u540e\u7eed\u8f93\u51fa\u53ef\u80fd\u7ee7\u7eed\u6cbf\u7528\u9519\u8bef\u540d\u79f0\uff0c\u4e0d\u80fd\u56e0\u4e3a\u7ed3\u679c\u6765\u81ea\u81ea\u52a8\u5316\u6d41\u7a0b\u5c31\u514d\u9664\u5bf9\u53c2\u8003\u6765\u6e90\u7684\u5ba1\u67e5\u3002<\/p>\n
\u6307\u5b9a\u86cb\u767d\u7684 FASTA \u6807\u9898\u683c\u5f0f\u4e5f\u9700\u8981\u6309\u5de5\u5177\u8981\u6c42\u51c6\u5907\u3002\u7b80\u5355\u683c\u5f0f\u53ea\u63d0\u4f9b\u6807\u8bc6\u548c\u4ea7\u7269\u8bf4\u660e\uff0c\u8be6\u7ec6\u683c\u5f0f\u53ef\u4ee5\u5199\u5165\u9176\u5b66\u7f16\u53f7\u3001\u57fa\u56e0\u540d\u79f0\u3001\u4ea7\u7269\u548c\u5176\u4ed6\u6ce8\u91ca\u3002\u672c\u6b21\u9a71\u52a8\u660e\u786e\u751f\u6210\u9002\u5408 Prokka \u7684\u6807\u9898\uff0c\u4e0d\u628a\u5176\u4ed6\u6ce8\u91ca\u5de5\u5177\u7684\u81ea\u5b9a\u4e49\u683c\u5f0f\u76f4\u63a5\u590d\u5236\u8fc7\u6765\u4f7f\u7528\u3002<\/p>\n
\u53ef\u4fe1\u53c2\u8003\u5e94\u5f53\u600e\u6837\u9009\u62e9<\/h2>\n
\u5728\u6b63\u5f0f\u9879\u76ee\u4e2d\uff0c\u4f18\u5148\u4f7f\u7528\u6765\u6e90\u660e\u786e\u3001\u529f\u80fd\u6ce8\u91ca\u7ecf\u8fc7\u9002\u5f53\u5ba1\u67e5\u4e14\u4e0e\u7814\u7a76\u5bf9\u8c61\u76f8\u5173\u7684\u86cb\u767d\u6216\u53c2\u8003\u57fa\u56e0\u7ec4\u3002\u9700\u8981\u4fdd\u7559\u53c2\u8003\u7248\u672c\u3001\u6765\u6e90\u548c\u52a0\u5de5\u6b65\u9aa4\uff0c\u5c24\u5176\u8981\u8bb0\u5f55\u662f\u5426\u4eba\u4e3a\u6539\u8fc7\u57fa\u56e0\u540d\u6216\u4ea7\u7269\u63cf\u8ff0\u3002<\/p>\n
\u4ece\u81ea\u5df1\u4e0a\u4e00\u8f6e\u81ea\u52a8\u6ce8\u91ca\u4e2d\u63d0\u53d6\u86cb\u767d\uff0c\u518d\u628a\u5b83\u4f5c\u4e3a\u53ef\u4fe1\u5e93\u91cd\u65b0\u6ce8\u91ca\uff0c\u53ef\u4ee5\u6539\u5584\u540d\u79f0\u4e00\u81f4\u6027\uff0c\u4f46\u4e0d\u4f1a\u81ea\u52a8\u589e\u52a0\u72ec\u7acb\u8bc1\u636e\u3002\u672c\u6b21\u523b\u610f\u590d\u5236\u57fa\u7ebf\u86cb\u767d\u5e76\u8d4b\u4e88\u6f14\u793a\u540d\u79f0\uff0c\u5c31\u662f\u4e3a\u4e86\u628a\u8fd9\u4e00\u70b9\u5c55\u793a\u6e05\u695a\u3002<\/p>\n
\u540d\u79f0\u4e00\u81f4\u4e5f\u4e0d\u662f\u8d28\u91cf\u7684\u552f\u4e00\u6807\u51c6\u3002\u4e24\u4e2a\u6837\u672c\u4f7f\u7528\u540c\u4e00\u4e2a\u9519\u8bef\u53c2\u8003\uff0c\u53ef\u4ee5\u5f97\u5230\u975e\u5e38\u6574\u9f50\u7684\u529f\u80fd\u8868\uff0c\u5374\u628a\u76f8\u540c\u7684\u504f\u5dee\u4f20\u9012\u5230\u6bcf\u4e2a\u6837\u672c\u3002\u6bd4\u8f83\u5206\u6790\u5e94\u540c\u65f6\u5173\u6ce8\u547d\u540d\u6765\u6e90\u3001\u8986\u76d6\u8303\u56f4\u4ee5\u53ca\u662f\u5426\u5b58\u5728\u53ea\u5f97\u5230\u90e8\u5206\u5339\u914d\u7684\u6761\u76ee\u3002<\/p>\n
\u5bf9\u7167\u7ed3\u679c\u5982\u4f55\u7528\u4e8e\u771f\u5b9e\u9879\u76ee<\/h2>\n
\u5982\u679c\u4e24\u6b21\u6ce8\u91ca\u57fa\u56e0\u603b\u6570\u76f8\u8fd1\uff0c\u5efa\u8bae\u5148\u62bd\u67e5\u53d8\u5316\u6700\u5927\u7684\u529f\u80fd\u7c7b\u522b\u3002\u6cbf\u7740\u5bf9\u5e94\u6761\u76ee\u68c0\u67e5\u6838\u9178\u662f\u5426\u76f8\u540c\u3001\u9884\u6d4b\u8fb9\u754c\u662f\u5426\u76f8\u540c\u3001\u86cb\u767d\u5e8f\u5217\u662f\u5426\u76f8\u540c\uff0c\u6700\u540e\u518d\u770b\u547d\u540d\u4f9d\u636e\u3002\u8fd9\u4e2a\u987a\u5e8f\u80fd\u5e2e\u52a9\u533a\u5206\u7ec4\u88c5\u53d8\u5316\u3001\u9884\u6d4b\u53d8\u5316\u4e0e\u540d\u79f0\u53d8\u5316\u3002<\/p>\n
\u5982\u679c\u86cb\u767d\u76f8\u540c\u4f46\u540d\u79f0\u4e0d\u540c\uff0c\u4f18\u5148\u68c0\u67e5\u6570\u636e\u5e93\u3001\u6307\u5b9a\u53c2\u8003\u3001\u5feb\u901f\u6a21\u5f0f\u548c\u540d\u79f0\u6574\u7406\u89c4\u5219\u3002\u5982\u679c\u86cb\u767d\u5df2\u7ecf\u4e0d\u540c\uff0c\u5219\u9700\u8981\u8fdb\u4e00\u6b65\u67e5\u770b\u8d77\u59cb\u4f4d\u70b9\u3001\u8bfb\u6846\u3001\u622a\u65ad\u548c\u7ec4\u88c5\u5185\u5bb9\uff0c\u800c\u4e0d\u80fd\u53ea\u4fee\u6539\u8868\u683c\u4e2d\u7684\u540d\u79f0\u4f7f\u4e24\u7248\u770b\u8d77\u6765\u4e00\u81f4\u3002<\/p>\n
\u5f53\u5047\u5b9a\u86cb\u767d\u6bd4\u4f8b\u660e\u663e\u4e0a\u5347\u65f6\uff0c\u4e5f\u5e94\u5148\u6838\u5bf9\u8fd0\u884c\u8303\u56f4\u3002\u8df3\u8fc7\u641c\u7d22\u3001\u53c2\u8003\u8986\u76d6\u4e0d\u8db3\u6216\u8f93\u5165\u53d8\u5316\u90fd\u53ef\u80fd\u5f71\u54cd\u8fd9\u4e2a\u6bd4\u4f8b\u3002\u53ea\u6709\u5728\u73af\u5883\u4e0e\u53c2\u6570\u53ef\u6bd4\u8f83\u3001\u6570\u636e\u8d28\u91cf\u4e5f\u5f97\u5230\u68c0\u67e5\u540e\uff0c\u624d\u9002\u5408\u8ba8\u8bba\u6837\u672c\u5c42\u9762\u7684\u5dee\u522b\u3002<\/p>\n
\u672c\u6b21\u6ca1\u6709\u7528\u56db\u7ec4\u8fd0\u884c\u8bc4\u4f30\u6ce8\u91ca\u51c6\u786e\u7387\uff0c\u6ca1\u6709\u628a\u6f14\u793a\u6807\u7b7e\u4f5c\u4e3a\u529f\u80fd\u9a8c\u8bc1\uff0c\u4e5f\u6ca1\u6709\u628a\u7247\u6bb5\u4e0a\u7684\u57fa\u56e0\u6570\u5916\u63a8\u5230\u5b8c\u6574\u57fa\u56e0\u7ec4\u3002\u5b83\u63d0\u4f9b\u7684\u662f\u4e00\u79cd\u53ef\u590d\u7528\u7684\u6838\u5bf9\u65b9\u6cd5\uff0c\u8ba9\u8bfb\u8005\u80fd\u591f\u628a\u6ce8\u91ca\u5dee\u5f02\u62c6\u5230\u5177\u4f53\u6587\u4ef6\u548c\u5177\u4f53\u53c2\u6570\uff0c\u800c\u4e0d\u662f\u53ea\u6bd4\u8f83\u6c47\u603b\u6570\u5b57\u3002<\/p>\n
\u590d\u73b0\u5305\u5305\u62ec\u516c\u5f00\u53c2\u8003\u6821\u9a8c\u89c4\u5219\u3001\u56db\u7ec4\u547d\u4ee4\u3001\u7279\u5f81\u5750\u6807\u3001\u86cb\u767d\u6821\u9a8c\u503c\u4ee5\u53ca\u53d1\u751f\u540d\u79f0\u53d8\u5316\u7684\u6761\u76ee\uff0c\u4fbf\u4e8e\u68c0\u67e5\u6bcf\u4e00\u4e2a\u7ed3\u8bba\u7684\u6765\u6e90\u3002\u4e0b\u8f7d\u811a\u672c\u4e0e\u5b9e\u8dd1\u7ed3\u679c<\/a><\/p>\n
\u9700\u8981\u6279\u91cf\u7ef4\u62a4\u83cc\u682a\u6ce8\u91ca\u3001\u7edf\u4e00\u53c2\u8003\u7248\u672c\u5e76\u6838\u5bf9\u8de8\u6279\u6b21\u7ed3\u679c\u65f6\uff0c\u53ef\u4e86\u89e3\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u8ba1\u7b97\u670d\u52a1<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u56fa\u5b9a\u4e00\u6bb5\u516c\u5f00\u53c2\u8003\uff0c\u5b9e\u8dd1\u666e\u901a\u3001\u5feb\u901f\u3001\u6539\u6807\u7b7e\u548c\u6307\u5b9a\u86cb\u767d\u56db\u7ec4\u5bf9\u7167\uff0c\u9010\u9879\u6838\u5bf9\u5750\u6807\u3001\u86cb\u767d\u6821\u9a8c\u503c\u3001\u529f\u80fd\u540d\u79f0\u4e0e\u5b9e\u9645\u641c\u7d22\u65e5\u5fd7\u3002<\/p>\n","protected":false},"author":1,"featured_media":72832,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[1298,597,510,1299],"class_list":["post-72833","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-prokka","tag-597","tag-510","tag-1299"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/prokka-fast-mode-locus-tags-trusted-protein-practice-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72833","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=72833"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72833\/revisions"}],"predecessor-version":[{"id":72838,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72833\/revisions\/72838"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/72832"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=72833"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=72833"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=72833"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}