{"id":72837,"date":"2026-11-15T11:33:34","date_gmt":"2026-11-15T03:33:34","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72837"},"modified":"2026-11-15T11:33:34","modified_gmt":"2026-11-15T03:33:34","slug":"panaroo-missing-annotation-refind-presence-absence-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/panaroo-missing-annotation-refind-presence-absence-practice\/","title":{"rendered":"Panaroo \u77e9\u9635\u4e2d\u7684\u7f3a\u5931\u57fa\u56e0\u771f\u7684\u4e0d\u5728\u5417\uff1f\u5220\u9664\u4e00\u6761\u6ce8\u91ca\uff0c\u5b9e\u8dd1\u68c0\u67e5\u91cd\u65b0\u5bfb\u627e\u6b65\u9aa4"},"content":{"rendered":"
\n

\u5728\u6cdb\u57fa\u56e0\u7ec4\u5b58\u5728\u7f3a\u5931\u77e9\u9635\u91cc\u770b\u5230\u4e00\u4e2a\u96f6\uff0c\u6700\u76f4\u63a5\u7684\u89e3\u91ca\u662f\u8be5\u6837\u672c\u6ca1\u6709\u76f8\u5e94\u57fa\u56e0\u3002\u4f46\u77e9\u9635\u901a\u5e38\u6765\u81ea\u7ec4\u88c5\u4e0e\u6ce8\u91ca\uff0c\u67d0\u4e2a\u4f4d\u7f6e\u6ca1\u6709\u88ab\u6ce8\u91ca\u51fa\u6765\uff0c\u4e5f\u53ef\u80fd\u4ea7\u751f\u770b\u8d77\u6765\u50cf\u7f3a\u5931\u7684\u8bb0\u5f55\u3002\u8981\u533a\u5206\u8fd9\u4e24\u79cd\u60c5\u51b5\uff0c\u5fc5\u987b\u91cd\u65b0\u68c0\u67e5\u5bf9\u5e94\u6838\u9178\uff0c\u800c\u4e0d\u80fd\u53ea\u76ef\u7740\u6700\u7ec8\u77e9\u9635\u3002<\/p>\n

\u672c\u6587\u5b89\u88c5\u5e76\u8fd0\u884c Panaroo 1.8.1\uff0c\u4f7f\u7528\u4e00\u6bb5\u7ecf\u8fc7\u5b9e\u9645 Prokka \u6ce8\u91ca\u7684\u516c\u5f00\u53c2\u8003\u5e8f\u5217\uff0c\u6784\u9020\u4e09\u4efd\u5bf9\u7167\u8f93\u5165\u3002\u4e09\u4efd\u6838\u9178\u5b8c\u5168\u4e00\u81f4\uff0c\u53ea\u4ece\u5176\u4e2d\u4e00\u4efd\u5220\u9664\u4e00\u6761 CDS \u6ce8\u91ca\uff0c\u518d\u6bd4\u8f83\u5173\u95ed\u5bfb\u627e\u3001\u9ed8\u8ba4\u5bfb\u627e\u548c\u4e25\u683c\u5bfb\u627e\u7b49\u8bbe\u7f6e\u3002\u5b9e\u9a8c\u5173\u6ce8\u4e00\u4e2a\u53ef\u4ee5\u76f4\u63a5\u6838\u5b9e\u7684\u95ee\u9898\uff0c\u540c\u4e00\u6bb5 DNA \u8fd8\u5728\u65f6\uff0c\u8f6f\u4ef6\u5982\u4f55\u5904\u7406\u6ce8\u91ca\u8868\u4e2d\u7684\u7f3a\u53e3\u3002<\/p>\n

\"\u77e9\u9635\u4e2d\u7684\u96f6\uff0c\u53ef\u80fd\u6765\u81ea\u6f0f\u6ce8\u91ca\u3002\u4fdd\u7559\u76f8\u540c
\u4fdd\u7559\u76f8\u540c DNA\uff0c\u53ea\u5220\u9664\u4e00\u6761 CDS \u6ce8\u91ca<\/figcaption><\/figure>\n

\u6cdb\u57fa\u56e0\u7ec4\u8f93\u5165\u4e2d\u6709\u4e24\u79cd\u4e0d\u540c\u7684\u4fe1\u606f<\/h2>\n

GFF \u6587\u4ef6\u8bb0\u5f55\u4e86\u9884\u6d4b\u7279\u5f81\u7684\u4f4d\u7f6e\u4e0e\u5c5e\u6027\uff0c\u5e8f\u5217\u90e8\u5206\u8bb0\u5f55\u8fd9\u4e9b\u4f4d\u7f6e\u5bf9\u5e94\u7684\u6838\u9178\u3002\u5b83\u4eec\u76f8\u4e92\u5173\u8054\uff0c\u4f46\u4e0d\u662f\u540c\u4e00\u4efd\u4fe1\u606f\u3002\u5220\u9664\u4e00\u6761\u6ce8\u91ca\u884c\uff0c\u4e0d\u4f1a\u81ea\u52a8\u5220\u9664\u8be5\u4f4d\u7f6e\u7684\u78b1\u57fa\uff0c\u5220\u9664\u57fa\u56e0\u7ec4\u7247\u6bb5\u4e5f\u4e0d\u80fd\u53ea\u9760\u5220\u6ce8\u91ca\u884c\u6765\u6a21\u62df\u3002<\/p>\n

Panaroo \u53ef\u4ee5\u7ed3\u5408\u57fa\u56e0\u805a\u7c7b\u548c\u76f8\u90bb\u5173\u7cfb\u5904\u7406\u90e8\u5206\u6ce8\u91ca\u4e0d\u4e00\u81f4\u3002\u5224\u65ad\u4e00\u4e2a\u7f3a\u53e3\u65f6\uff0c\u5b83\u6709\u673a\u4f1a\u5229\u7528\u90bb\u8fd1\u57fa\u56e0\u9644\u8fd1\u7684\u6838\u9178\u5bfb\u627e\u539f\u5148\u6ca1\u6709\u6807\u51fa\u7684\u5185\u5bb9\u3002\u56e0\u6b64\uff0c\u7ed3\u679c\u4e2d\u67d0\u4e2a\u6761\u76ee\u91cd\u65b0\u51fa\u73b0\uff0c\u9700\u8981\u89e3\u91ca\u5b83\u6765\u81ea\u539f\u59cb\u6ce8\u91ca\u8fd8\u662f\u540e\u7eed\u5bfb\u627e\u3002\u5b98\u65b9\u53c2\u6570\u8bf4\u660e<\/a><\/p>\n

\u8fd9\u4e5f\u51b3\u5b9a\u4e86\u590d\u73b0\u6750\u6599\u4e0d\u80fd\u53ea\u5269\u4e00\u5f20\u86cb\u767d\u540d\u79f0\u8868\u3002\u8f93\u5165\u7684\u7279\u5f81\u5750\u6807\u3001\u5e8f\u5217\u3001\u6837\u672c\u5bf9\u5e94\u5173\u7cfb\u4ee5\u53ca\u5173\u952e\u53c2\u6570\u90fd\u9700\u8981\u4fdd\u5b58\uff0c\u5426\u5219\u5f88\u96be\u8ffd\u67e5\u77e9\u9635\u4e2d\u7684\u96f6\u4e3a\u4f55\u6539\u53d8\u3002<\/p>\n

\u5b89\u88c5\u7248\u672c\u4e0e\u672c\u6b21\u8f93\u5165\u8303\u56f4<\/h2>\n

\u672c\u6b21\u4f7f\u7528\u56fa\u5b9a\u7248\u672c\u7684 BioContainers \u955c\u50cf\uff0c\u5b9e\u9645\u7248\u672c\u8f93\u51fa\u4e3a 1.8.1\u3002\u590d\u73b0\u5305\u8bb0\u5f55\u955c\u50cf\u6458\u8981\uff0c\u5e76\u63d0\u4f9b\u4ece\u57fa\u7ebf\u6ce8\u91ca\u751f\u6210\u4e09\u4efd\u8f93\u5165\u3001\u6267\u884c\u56db\u7ec4\u547d\u4ee4\u548c\u89e3\u6790\u7ed3\u679c\u7684\u811a\u672c\u3002<\/p>\n

PANAROO_IMAGE='quay.io\/biocontainers\/panaroo:1.8.1--pyhdfd78af_0'\r\ndocker pull "$PANAROO_IMAGE"\r\ndocker run --rm "$PANAROO_IMAGE" panaroo --version\r\n<\/code><\/pre>\n
\u57fa\u7ebf\u6765\u81ea\u516c\u5f00\u5927\u80a0\u6746\u83cc\u53c2\u8003 NC_000913.3 \u7684\u524d\u4e8c\u5341\u4e07\u78b1\u57fa\uff0c\u4f7f\u7528\u5b9e\u9645 Prokka \u8f93\u51fa\u7684\u4e3b\u6ce8\u91ca\u6587\u4ef6\u548c\u86cb\u767d\u6587\u4ef6\u3002\u8fd9\u91cc\u53ea\u5206\u6790\u4e00\u4e2a\u8fde\u7eed\u7247\u6bb5\uff0c\u4e09\u4efd\u8f93\u5165\u90fd\u662f\u4e3a\u53c2\u6570\u5b9e\u9a8c\u6784\u9020\u7684\u526f\u672c\uff0c\u4e0d\u662f\u4e09\u4e2a\u72ec\u7acb\u83cc\u682a\uff0c\u4e5f\u4e0d\u662f\u4e09\u4efd\u751f\u7269\u5b66\u91cd\u590d\u3002<\/p>\n
\u56e0\u6b64\uff0c\u672c\u6587\u4e0d\u4f1a\u7528\u8fd9\u7ec4\u4e09\u4efd\u6570\u636e\u4f30\u8ba1\u67d0\u4e2a\u7269\u79cd\u7684\u6cdb\u57fa\u56e0\u7ec4\u5927\u5c0f\uff0c\u4e0d\u8ba8\u8bba\u771f\u5b9e\u7fa4\u4f53\u4e2d\u7684\u57fa\u56e0\u9891\u7387\uff0c\u4e5f\u4e0d\u8fdb\u884c\u5173\u8054\u5206\u6790\u3002\u8bbe\u8ba1\u526f\u672c\u7684\u7528\u9014\u662f\u63a7\u5236\u53d8\u91cf\uff0c\u8ba9\u6211\u4eec\u80fd\u591f\u77e5\u9053\u67d0\u4e2a\u7f3a\u5931\u662f\u5426\u771f\u7684\u53d1\u751f\u5728\u6838\u9178\u5c42\u9762\u3002<\/p>\n
\u53ea\u5220\u9664\u6ce8\u91ca\uff0c\u5177\u4f53\u600e\u6837\u64cd\u4f5c<\/h2>\n
\u9a71\u52a8\u5728\u57fa\u7ebf\u4e2d\u5bfb\u627e\u4f4d\u4e8e\u7247\u6bb5\u5185\u90e8\u3001\u957f\u5ea6\u9002\u4e2d\u4e14\u86cb\u767d\u5e8f\u5217\u4e0d\u91cd\u590d\u7684 CDS\uff0c\u907f\u514d\u628a\u622a\u53d6\u8fb9\u754c\u6216\u76f8\u540c\u86cb\u767d\u7684\u591a\u4e2a\u62f7\u8d1d\u540c\u65f6\u5f15\u5165\u4e3b\u5bf9\u7167\u3002\u672c\u6b21\u9009\u4e2d\u7684\u76ee\u6807\u7f16\u53f7\u4e3a BASE_00044\uff0c\u4f4d\u7f6e\u4e3a 50380 \u5230 51222\uff0c\u957f\u5ea6\u4e3a 843 \u78b1\u57fa\u3002<\/p>\n
\u4e09\u4efd\u8f93\u5165\u5206\u522b\u53eb sample_A\u3001sample_B \u548c sample_C\u3002\u6bcf\u4efd\u91c7\u7528\u4e0d\u540c\u7684\u6761\u76ee\u7f16\u53f7\u524d\u7f00\uff0c\u4fbf\u4e8e\u8bc6\u522b\u6765\u6e90\u3002\u53ea\u6709\u6837\u672c B \u5220\u9664\u76ee\u6807 CDS \u5bf9\u5e94\u7684\u6ce8\u91ca\u884c\uff0c\u53e6\u4e24\u4efd\u4fdd\u7559\u539f\u6765\u7684\u6ce8\u91ca\u3002<\/p>\n
\u4e09\u4efd\u6587\u4ef6\u4e2d\u7684\u6838\u9178\u90e8\u5206\u9010\u5b57\u76f8\u540c\uff0c\u6821\u9a8c\u503c\u4e5f\u76f8\u540c\u3002\u8f93\u5165 CDS \u6570\u91cf\u5206\u522b\u4e3a 169\u3001168\u3001169\u3002\u8fd9\u91cc\u51cf\u5c11\u7684\u4e00\u6761\u8bb0\u5f55\u5c5e\u4e8e\u6ce8\u91ca\u5dee\u5f02\uff0c\u4e0d\u80fd\u5728\u5b9e\u9a8c\u63cf\u8ff0\u91cc\u5199\u6210\u6837\u672c B \u53d1\u751f\u4e86\u57fa\u56e0\u7f3a\u5931\u7a81\u53d8\u3002<\/p>\n
\u8fd9\u79cd\u51c6\u5907\u65b9\u5f0f\u6bd4\u76f4\u63a5\u4fee\u6539\u6700\u7ec8\u77e9\u9635\u66f4\u6709\u610f\u4e49\u3002\u6211\u4eec\u6539\u53d8\u7684\u662f\u5de5\u5177\u771f\u6b63\u8bfb\u53d6\u7684\u8f93\u5165\uff0c\u7136\u540e\u7531\u5de5\u5177\u91cd\u65b0\u4ea7\u751f\u7ed3\u679c\uff0c\u80fd\u591f\u89c2\u5bdf\u5b8c\u6574\u5904\u7406\u8fc7\u7a0b\u4e2d\u54ea\u4e9b\u6b65\u9aa4\u6539\u53d8\u4e86\u5224\u65ad\u3002<\/p>\n
\u5148\u5173\u95ed\u5bfb\u627e\uff0c\u89c2\u5bdf\u7f3a\u53e3\u5982\u4f55\u8fdb\u5165\u77e9\u9635<\/h2>\n
\u7b2c\u4e00\u7ec4\u56fa\u5b9a\u4e25\u683c\u6e05\u7406\u6a21\u5f0f\uff0c\u5e76\u660e\u786e\u5173\u95ed\u91cd\u65b0\u5bfb\u627e\u6b65\u9aa4\u3002\u8fd9\u6837\u80fd\u591f\u5efa\u7acb\u4e00\u4efd\u6ca1\u6709\u8fd9\u4e00\u6b65\u8865\u5145\u4fe1\u606f\u7684\u5bf9\u7167\u3002<\/p>\n
panaroo -i inputs\/sample_A.gff inputs\/sample_B.gff inputs\/sample_C.gff \\\r\n  -o strict_off --clean-mode strict --refind-mode off -t 2\r\n<\/code><\/pre>\n
\u5b9e\u9645\u4e8c\u503c\u77e9\u9635\u4e2d\uff0c\u76ee\u6807\u6761\u76ee\u5728\u4e09\u4e2a\u6837\u672c\u7684\u8bb0\u5f55\u4e3a 1\u30010\u30011\u3002\u8fd9\u4e0e\u8f93\u5165\u6ce8\u91ca\u4e2d\u7684\u8bbe\u8ba1\u7f3a\u53e3\u5bf9\u5e94\uff0c\u8bf4\u660e\u53ea\u770b\u8fd9\u4e00\u7248\u8f93\u51fa\uff0c\u8bfb\u8005\u786e\u5b9e\u4f1a\u5728\u6837\u672c B \u7684\u76ee\u6807\u4f4d\u7f6e\u770b\u5230\u7f3a\u5931\u3002<\/p>\n
\u4f46\u6211\u4eec\u5df2\u7ecf\u77e5\u9053\u4e09\u4efd\u8f93\u5165\u6838\u9178\u4e00\u81f4\uff0c\u56e0\u6b64\u8fd9\u4e2a\u96f6\u4e0d\u80fd\u7528\u4f5c\u771f\u5b9e DNA \u7f3a\u5931\u7684\u8bc1\u636e\u3002\u5b83\u9996\u5148\u8868\u660e\uff0c\u5728\u5f53\u524d\u8f93\u5165\u4e0e\u5904\u7406\u8bbe\u7f6e\u4e0b\uff0c\u6837\u672c B \u6ca1\u6709\u5f62\u6210\u76f8\u5e94\u7684\u57fa\u56e0\u5bb6\u65cf\u5b58\u5728\u8bb0\u5f55\u3002<\/p>\n
\u771f\u5b9e\u9879\u76ee\u6ca1\u6709\u8fd9\u6837\u7684\u5df2\u77e5\u7b54\u6848\uff0c\u6240\u4ee5\u9700\u8981\u8fdb\u4e00\u6b65\u68c0\u67e5\u539f\u59cb\u7ec4\u88c5\u3001\u5c40\u90e8\u6bd4\u5bf9\u4e0e\u6d4b\u5e8f\u8986\u76d6\u3002\u4e00\u4e2a\u4e8c\u503c\u77e9\u9635\u5355\u5143\u683c\u627f\u8f7d\u4e0d\u4e86\u6240\u6709\u4e0d\u786e\u5b9a\u6027\uff0c\u62a5\u544a\u4e2d\u5e94\u4fdd\u7559\u5176\u5f62\u6210\u8fc7\u7a0b\u548c\u540e\u7eed\u6838\u5bf9\u4f9d\u636e\u3002<\/p>\n
\u5f00\u542f\u9ed8\u8ba4\u5bfb\u627e\u4ee5\u540e\u53d1\u751f\u4e86\u4ec0\u4e48<\/h2>\n
\u7b2c\u4e8c\u7ec4\u4ecd\u91c7\u7528\u4e25\u683c\u6e05\u7406\u6a21\u5f0f\uff0c\u53ea\u628a\u5bfb\u627e\u6a21\u5f0f\u6539\u4e3a\u9ed8\u8ba4\u3002\u5176\u4ed6\u8f93\u5165\u548c\u4e3b\u8981\u8bbe\u7f6e\u4fdd\u6301\u4e00\u81f4\u3002<\/p>\n
panaroo -i inputs\/sample_A.gff inputs\/sample_B.gff inputs\/sample_C.gff \\\r\n  -o strict_default --clean-mode strict --refind-mode default -t 2\r\n<\/code><\/pre>\n
\u672c\u6b21\u76ee\u6807\u6761\u76ee\u7684\u4e8c\u503c\u8bb0\u5f55\u53d8\u4e3a 1\u30011\u30011\u3002\u6837\u672c B \u7684\u539f\u59cb GFF \u4e2d\u4ecd\u7136\u6ca1\u6709\u90a3\u6761 CDS \u6ce8\u91ca\uff0c\u4f46\u8f6f\u4ef6\u5229\u7528\u8f93\u5165\u5e8f\u5217\u548c\u5468\u56f4\u7684\u57fa\u56e0\u5173\u7cfb\u627e\u56de\u4e86\u5bf9\u5e94\u5185\u5bb9\u3002\u8fd9\u4e2a\u53d8\u5316\u53ef\u4ee5\u8ffd\u6eaf\u5230\u91cd\u65b0\u5bfb\u627e\u6b65\u9aa4\u3002<\/p>\n
\u7406\u89e3\u7ed3\u679c\u65f6\uff0c\u9700\u8981\u628a\u201c\u539f\u5148\u88ab\u6ce8\u91ca\u51fa\u6765\u201d\u548c\u201c\u540e\u7eed\u901a\u8fc7\u5bfb\u627e\u6062\u590d\u201d\u5206\u5f00\u3002\u4e24\u8005\u90fd\u53ef\u80fd\u8fdb\u5165\u6700\u540e\u7684\u5b58\u5728\u7f3a\u5931\u8868\uff0c\u4f46\u8bc1\u636e\u8def\u5f84\u4e0d\u540c\u3002\u82e5\u540e\u7eed\u7814\u7a76\u9700\u8981\u5ba1\u67e5\u67d0\u4e2a\u5173\u952e\u57fa\u56e0\uff0c\u5e94\u8be5\u7ee7\u7eed\u67e5\u770b\u76ee\u6807\u5e8f\u5217\u4e0e\u5bf9\u5e94\u8f93\u51fa\u8bb0\u5f55\u3002<\/p>\n
\u540c\u6837\uff0c\u91cd\u65b0\u627e\u5230\u6838\u9178\u7247\u6bb5\u5e76\u4e0d\u81ea\u52a8\u8bc1\u660e\u5b83\u5177\u6709\u5b8c\u6574\u529f\u80fd\u3002\u9ed8\u8ba4\u5bfb\u627e\u53ef\u4ee5\u5bb9\u7eb3\u90e8\u5206\u6ce8\u91ca\u6216\u7ec4\u88c5\u5f02\u5e38\uff0c\u76f8\u5173\u5e8f\u5217\u8fd8\u9700\u8981\u68c0\u67e5\u957f\u5ea6\u3001\u8bfb\u6846\u3001\u7ec8\u6b62\u5bc6\u7801\u5b50\u548c\u8986\u76d6\u60c5\u51b5\uff0c\u5c24\u5176\u4e0d\u80fd\u4ec5\u51ed\u5b58\u5728\u8bb0\u5f55\u5c31\u65ad\u8a00\u86cb\u767d\u5df2\u7ecf\u8868\u8fbe\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
\u8bbe\u7f6e<\/th>\n\u6e05\u7406\u6a21\u5f0f<\/th>\n\u76ee\u6807\u5728\u4e09\u4efd\u8f93\u5165\u4e2d\u7684\u8bb0\u5f55<\/th>\n<\/tr>\n<\/thead>\n
\u5173\u95ed\u5bfb\u627e<\/td>\n\u4e25\u683c<\/td>\n1\u30010\u30011<\/td>\n<\/tr>\n
\u9ed8\u8ba4\u5bfb\u627e<\/td>\n\u4e25\u683c<\/td>\n1\u30011\u30011<\/td>\n<\/tr>\n
\u4e25\u683c\u5bfb\u627e<\/td>\n\u4e25\u683c<\/td>\n1\u30011\u30011<\/td>\n<\/tr>\n
\u9ed8\u8ba4\u5bfb\u627e<\/td>\n\u654f\u611f<\/td>\n1\u30011\u30011<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u4e25\u683c\u5bfb\u627e\u4e0e\u4e25\u683c\u6e05\u7406\u662f\u4e24\u9879\u4e0d\u540c\u8bbe\u7f6e<\/h2>\n
\u7b2c\u4e09\u7ec4\u628a\u5bfb\u627e\u6a21\u5f0f\u8bbe\u4e3a\u4e25\u683c\uff0c\u540c\u65f6\u7ee7\u7eed\u4f7f\u7528\u4e25\u683c\u6e05\u7406\u6a21\u5f0f\u3002\u8fd9\u4e24\u4e2a\u53c2\u6570\u540d\u5b57\u76f8\u4f3c\uff0c\u4f46\u63a7\u5236\u7684\u662f\u4e0d\u540c\u73af\u8282\u3002\u524d\u8005\u9650\u5236\u54ea\u4e9b\u5019\u9009\u5e8f\u5217\u80fd\u591f\u88ab\u91cd\u65b0\u627e\u56de\uff0c\u540e\u8005\u5f71\u54cd\u57fa\u56e0\u56fe\u548c\u4f4e\u652f\u6301\u6761\u76ee\u7b49\u6e05\u7406\u51b3\u7b56\u3002<\/p>\n
panaroo -i inputs\/sample_A.gff inputs\/sample_B.gff inputs\/sample_C.gff \\\r\n  -o strict_refind_strict --clean-mode strict --refind-mode strict -t 2\r\n<\/code><\/pre>\n
\u672c\u6b21\u76ee\u6807\u5728\u4e25\u683c\u5bfb\u627e\u4e0b\u4ecd\u5f97\u5230 1\u30011\u30011\uff0c\u4e0e\u9ed8\u8ba4\u5bfb\u627e\u7684\u76ee\u6807\u7ed3\u679c\u4e00\u81f4\u3002\u7531\u4e8e\u672c\u4f8b\u4fdd\u7559\u7684\u662f\u5b8c\u6574\u3001\u76f8\u540c\u7684\u6838\u9178\u5185\u5bb9\uff0c\u8fd9\u4e2a\u73b0\u8c61\u7b26\u5408\u5b9e\u9a8c\u8bbe\u8ba1\u3002\u5b83\u4e0d\u80fd\u8bf4\u660e\u4e24\u79cd\u5bfb\u627e\u6a21\u5f0f\u5bf9\u6240\u6709\u771f\u5b9e\u6570\u636e\u90fd\u4f1a\u7ed9\u51fa\u540c\u6837\u7ed3\u679c\u3002<\/p>\n
\u5f53\u5f85\u5bfb\u627e\u533a\u57df\u5b58\u5728\u79fb\u7801\u3001\u5185\u90e8\u7ec8\u6b62\u6216\u7ec4\u88c5\u65ad\u88c2\u65f6\uff0c\u6a21\u5f0f\u9009\u62e9\u53ef\u80fd\u6539\u53d8\u7ed3\u679c\u3002\u8bfb\u8005\u5e94\u6839\u636e\u7814\u7a76\u76ee\u7684\u5224\u65ad\u81ea\u5df1\u8981\u7edf\u8ba1\u7684\u662f\u53ef\u8fa8\u8ba4\u7684\u540c\u6e90\u5e8f\u5217\uff0c\u8fd8\u662f\u66f4\u63a5\u8fd1\u5b8c\u6574\u7f16\u7801\u80fd\u529b\u7684\u5019\u9009\uff0c\u5e76\u5728\u65b9\u6cd5\u4e2d\u5199\u660e\u91c7\u7528\u7684\u8bbe\u7f6e\u3002<\/p>\n
\u654f\u611f\u6a21\u5f0f\u4e5f\u4e0d\u80fd\u81ea\u52a8\u56de\u7b54\u751f\u7269\u5b66\u95ee\u9898<\/h2>\n
\u7b2c\u56db\u7ec4\u91c7\u7528\u654f\u611f\u6e05\u7406\u6a21\u5f0f\uff0c\u540c\u65f6\u4fdd\u7559\u9ed8\u8ba4\u5bfb\u627e\u3002\u672c\u6b21\u76ee\u6807\u8bb0\u5f55\u4e3a 1\u30011\u30011\u3002\u8fd9\u4e2a\u5bf9\u7167\u5e2e\u52a9\u6211\u4eec\u68c0\u67e5\u4e3b\u7ed3\u8bba\u662f\u5426\u4f9d\u8d56\u67d0\u4e00\u79cd\u6e05\u7406\u8bbe\u7f6e\uff0c\u4f46\u4e0d\u80fd\u4ee3\u66ff\u5bf9\u5168\u90e8\u57fa\u56e0\u5bb6\u65cf\u7684\u5ba1\u67e5\u3002<\/p>\n
\u6e05\u7406\u6a21\u5f0f\u4f1a\u5f71\u54cd\u54ea\u4e9b\u4f4e\u652f\u6301\u5185\u5bb9\u88ab\u4fdd\u7559\u3002\u7a00\u6709\u8d28\u7c92\u548c\u6c61\u67d3\u6765\u6e90\u5728\u67d0\u4e9b\u60c5\u5f62\u4e0b\u90fd\u53ef\u80fd\u8868\u73b0\u4e3a\u5c11\u91cf\u6837\u672c\u4e2d\u7684\u7279\u6b8a\u5e8f\u5217\uff0c\u6240\u4ee5\u4fdd\u7559\u66f4\u591a\u7ed3\u679c\u5e76\u4e0d\u5929\u7136\u7b49\u4e8e\u66f4\u51c6\u786e\uff0c\u5220\u6389\u66f4\u591a\u7ed3\u679c\u4e5f\u4e0d\u5929\u7136\u7b49\u4e8e\u66f4\u53ef\u9760\u3002<\/p>\n
\u5b9e\u9645\u7814\u7a76\u4e2d\uff0c\u5982\u679c\u5173\u6ce8\u7f55\u89c1\u9644\u5c5e\u57fa\u56e0\uff0c\u5e94\u8be5\u5c06\u5019\u9009\u5bf9\u5e94\u7684\u6837\u672c\u8d28\u91cf\u3001\u5c40\u90e8\u5e8f\u5217\u548c\u8986\u76d6\u8bc1\u636e\u4e00\u8d77\u67e5\u770b\u3002\u6a21\u5f0f\u63d0\u4f9b\u7684\u662f\u5206\u6790\u53d6\u820d\uff0c\u65e0\u6cd5\u51ed\u7a7a\u8865\u5145\u5b9e\u9a8c\u4e2d\u4e0d\u5b58\u5728\u7684\u8bc1\u636e\u3002\u5b98\u65b9\u5feb\u901f\u5165\u95e8\u4e0e\u6a21\u5f0f\u8bf4\u660e<\/a><\/p>\n
\u8be6\u7ec6\u8868\u4e0e\u4e8c\u503c\u8868\u8981\u4e00\u8d77\u770b<\/h2>\n
\u4e8c\u503c\u8868\u9002\u5408\u5feb\u901f\u6c47\u603b\u6bcf\u4e2a\u57fa\u56e0\u5bb6\u65cf\u5728\u54ea\u4e9b\u6837\u672c\u51fa\u73b0\uff0c\u4f46\u5b83\u538b\u7f29\u4e86\u5177\u4f53\u6761\u76ee\u8eab\u4efd\u3002\u8be6\u7ec6\u5b58\u5728\u7f3a\u5931\u8868\u4fdd\u7559\u4e86\u6837\u672c\u4e2d\u7684\u5bf9\u5e94\u5e8f\u5217\u540d\u79f0\uff0c\u65b9\u4fbf\u7ee7\u7eed\u8ffd\u6eaf\u5230\u6ce8\u91ca\u4e0e\u86cb\u767d\u3002<\/p>\n
\u672c\u6b21\u9a71\u52a8\u5148\u6839\u636e\u6837\u672c A \u4e2d\u672a\u6539\u52a8\u7684\u76ee\u6807\u7f16\u53f7\u627e\u5230\u8be6\u7ec6\u8868\u4e2d\u7684\u5bb6\u65cf\uff0c\u518d\u8bfb\u53d6\u540c\u4e00\u5bb6\u65cf\u7684\u4e8c\u503c\u8bb0\u5f55\u3002\u8fd9\u6837\u907f\u514d\u6309\u53d8\u5316\u540e\u7684\u663e\u793a\u540d\u79f0\u731c\u6d4b\u76ee\u6807\uff0c\u4e5f\u907f\u514d\u628a\u4e24\u4e2a\u76f8\u4f3c\u529f\u80fd\u540d\u79f0\u8bef\u5f53\u6210\u540c\u4e00\u4e2a\u5bb6\u65cf\u3002<\/p>\n
\u8be6\u7ec6\u8868\u4e2d\u4e00\u4e2a\u6837\u672c\u5355\u5143\u683c\u8fd8\u53ef\u80fd\u5305\u542b\u591a\u4e2a\u7f16\u53f7\u3002\u5b58\u5728\u8bb0\u4e3a\u4e00\uff0c\u5e76\u4e0d\u5fc5\u7136\u8bf4\u660e\u8be5\u5bb6\u65cf\u5728\u6837\u672c\u4e2d\u53ea\u6709\u4e00\u4e2a\u62f7\u8d1d\u3002\u5f00\u5c55\u62f7\u8d1d\u6570\u76f8\u5173\u5206\u6790\u65f6\uff0c\u5e94\u8fd4\u56de\u5bf9\u5e94\u6210\u5458\u8bb0\u5f55\u6838\u5bf9\uff0c\u800c\u4e0d\u80fd\u628a\u5b58\u5728\u7f3a\u5931\u8868\u5f53\u4f5c\u5b9a\u91cf\u62f7\u8d1d\u6570\u77e9\u9635\u3002<\/p>\n
\u5982\u4f55\u8ffd\u8e2a\u91cd\u65b0\u627e\u5230\u7684\u5e8f\u5217<\/h2>\n
Panaroo \u8f93\u51fa\u7684\u6838\u9178\u4e0e\u86cb\u767d\u96c6\u5408\u53ef\u4ee5\u5305\u542b\u539f\u59cb\u6ce8\u91ca\u548c\u540e\u7eed\u5bfb\u627e\u5f97\u5230\u7684\u5185\u5bb9\u3002\u5185\u90e8\u7f16\u53f7\u4e0e\u539f\u59cb\u7f16\u53f7\u4e4b\u95f4\u8fd8\u9700\u8981\u901a\u8fc7\u5bf9\u5e94\u4fe1\u606f\u8fde\u63a5\uff0c\u56e0\u6b64\u4e0d\u80fd\u770b\u5230\u4e00\u4e2a\u964c\u751f\u7f16\u53f7\u5c31\u8ba4\u5b9a\u8f6f\u4ef6\u51ed\u7a7a\u4ea7\u751f\u4e86\u5e8f\u5217\u3002\u5b98\u65b9\u8f93\u51fa\u6587\u4ef6\u8bf4\u660e<\/a><\/p>\n
\u5bf9\u4e00\u4e2a\u91cd\u8981\u5019\u9009\uff0c\u53ef\u4ee5\u5148\u5728\u8be6\u7ec6\u5b58\u5728\u7f3a\u5931\u8868\u4e2d\u5b9a\u4f4d\u5bb6\u65cf\uff0c\u518d\u67e5\u5bf9\u5e94\u5e8f\u5217\u548c\u6765\u6e90\u8bb0\u5f55\uff0c\u6700\u540e\u56de\u5230\u6837\u672c\u7ec4\u88c5\u67e5\u770b\u5750\u6807\u9644\u8fd1\u7684\u6838\u9178\u3002\u672c\u4f8b\u4fdd\u7559\u5b8c\u6574\u7ed3\u679c\u5b57\u6bb5\uff0c\u8bfb\u8005\u53ef\u4ee5\u6309\u8fd9\u4e00\u6761\u8def\u5f84\u8ffd\u8e2a\u76ee\u6807\uff0c\u800c\u4e0d\u662f\u53ea\u76f8\u4fe1\u6b63\u6587\u4e2d\u7684\u4e00\u4e2a\u4e00\u6216\u96f6\u3002<\/p>\n
\u5982\u679c\u5b9e\u9645\u9879\u76ee\u4e2d\u5bfb\u627e\u7ed3\u679c\u548c\u539f\u59cb\u6ce8\u91ca\u76f8\u4e92\u77db\u76fe\uff0c\u5e94\u68c0\u67e5\u662f\u5426\u5bf9\u5e94\u540c\u4e00\u7ec4\u88c5\u7248\u672c\u3001\u540c\u4e00\u5e8f\u5217\u547d\u540d\u548c\u540c\u4e00\u5750\u6807\u7cfb\u7edf\u3002\u53ea\u66ff\u6362 GFF \u6216\u53ea\u66ff\u6362 FASTA \u90fd\u53ef\u80fd\u7834\u574f\u4e8c\u8005\u5173\u7cfb\uff0c\u4ea7\u751f\u96be\u4ee5\u89e3\u91ca\u7684\u9519\u8bef\u3002<\/p>\n
\u603b\u5bb6\u65cf\u6570\u6ca1\u6709\u53d8\uff0c\u4e5f\u53ef\u80fd\u6539\u53d8\u6838\u5fc3\u7edf\u8ba1<\/h2>\n
\u56db\u7ec4\u8fd0\u884c\u7684\u5bb6\u65cf\u603b\u6570\u90fd\u4e3a\u4e00\u767e\u516d\u5341\u4e5d\uff0c\u4f46\u5173\u95ed\u5bfb\u627e\u65f6\uff0c\u6c47\u603b\u6587\u4ef6\u628a\u4e00\u767e\u516d\u5341\u516b\u4e2a\u5bb6\u65cf\u5217\u4e3a\u6838\u5fc3\uff0c\u53e6\u4e00\u4e2a\u5217\u5165\u4e2d\u95f4\u9891\u7387\u7c7b\u522b\u3002\u5f00\u542f\u5bfb\u627e\u540e\uff0c\u4e00\u767e\u516d\u5341\u4e5d\u4e2a\u5bb6\u65cf\u90fd\u51fa\u73b0\u5728\u4e09\u4efd\u8f93\u5165\u4e2d\uff0c\u6838\u5fc3\u7edf\u8ba1\u968f\u4e4b\u589e\u52a0\u3002\u603b\u5bb6\u65cf\u6570\u76f8\u540c\uff0c\u5e76\u4e0d\u8868\u793a\u5b58\u5728\u7f3a\u5931\u7ed3\u6784\u76f8\u540c\u3002<\/p>\n
\u76ee\u6807\u5bb6\u65cf\u5728\u8be6\u7ec6\u8868\u4e2d\u53eb apaH\u3002\u5173\u95ed\u5bfb\u627e\u65f6\uff0c\u6837\u672c B \u5bf9\u5e94\u5355\u5143\u683c\u4e3a\u7a7a\uff0c\u5f00\u542f\u540e\u51fa\u73b0\u7f16\u53f7 1_refound_0<\/code>\u3002\u6211\u4eec\u8fdb\u4e00\u6b65\u6838\u5bf9\u5185\u90e8\u8bb0\u5f55\uff0c\u627e\u56de\u7684\u6838\u9178\u957f\u516b\u767e\u56db\u5341\u4e09\u78b1\u57fa\uff0c\u6821\u9a8c\u503c\u4e0e\u6837\u672c A\u3001C \u7684\u76ee\u6807\u5b8c\u5168\u4e00\u81f4\u3002\u8fd9\u6837\u53ef\u4ee5\u628a\u77e9\u9635\u4e2d\u7684\u53d8\u5316\u843d\u5b9e\u5230\u5177\u4f53\u5e8f\u5217\u8bc1\u636e\u3002<\/p>\n
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