{"id":72913,"date":"2026-11-15T12:17:49","date_gmt":"2026-11-15T04:17:49","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72913"},"modified":"2026-11-15T12:17:49","modified_gmt":"2026-11-15T04:17:49","slug":"bactopia-qc-sample-failure-resume-cache-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/bactopia-qc-sample-failure-resume-cache-practice\/","title":{"rendered":"Bactopia \u5b9e\u8dd1\u6559\u7a0b\uff5c\u6d41\u7a0b\u6210\u529f\u4e0d\u7b49\u4e8e\u6837\u672c\u5408\u683c\uff0c\u600e\u6837\u8bfb\u61c2\u8d28\u63a7\u6807\u8bb0\u4e0e\u6062\u590d\u8fd0\u884c"},"content":{"rendered":"
\n

\u628a\u4e00\u6279\u7ec6\u83cc\u6d4b\u5e8f\u6570\u636e\u4ea4\u7ed9\u81ea\u52a8\u6d41\u7a0b\u540e\uff0c\u6700\u8ba9\u4eba\u653e\u5fc3\u7684\u753b\u9762\u901a\u5e38\u662f\u7eff\u8272\u7684\u5b8c\u6210\u63d0\u793a\u3002\u7136\u800c\uff0c\u8fd9\u4e2a\u63d0\u793a\u9996\u5148\u56de\u7b54\u7684\u662f\u4efb\u52a1\u6709\u6ca1\u6709\u6309\u7a0b\u5e8f\u7ed3\u675f\uff0c\u5e76\u4e0d\u81ea\u52a8\u56de\u7b54\u6bcf\u4e2a\u6837\u672c\u662f\u5426\u8db3\u4ee5\u5f00\u5c55\u540e\u7eed\u5206\u6790\u3002\u672c\u6b21\u5b9e\u9645\u5b89\u88c5\u5e76\u8fd0\u884c Bactopia\uff0c\u6545\u610f\u653e\u5165\u4e00\u4e2a\u6570\u636e\u91cf\u5145\u8db3\u7684\u6559\u5b66\u6837\u672c\u548c\u4e00\u4e2a\u6570\u636e\u91cf\u660e\u663e\u4e0d\u8db3\u7684\u5bf9\u7167\u3002\u4e24\u6b21\u5de5\u4f5c\u6d41\u9000\u51fa\u7801\u90fd\u4e3a\u96f6\uff0c\u4f4e\u6570\u636e\u91cf\u6837\u672c\u5374\u7559\u4e0b\u4e86\u591a\u4efd\u8d28\u91cf\u9519\u8bef\u6587\u4ef6\u3002\u8fd9\u4e0d\u662f\u524d\u540e\u77db\u76fe\uff0c\u800c\u662f\u6279\u91cf\u5206\u6790\u5fc5\u987b\u7406\u89e3\u7684\u72b6\u6001\u5c42\u6b21\u3002<\/p>\n

\u6587\u7ae0\u91cd\u70b9\u662f\u5b9e\u9645\u8fd0\u884c\u8d28\u63a7\u6d41\u7a0b\u3001\u8fa8\u8ba4\u8f93\u51fa\u3001\u68c0\u67e5\u6062\u590d\u8fd0\u884c\u662f\u5426\u590d\u7528\u4e86\u4efb\u52a1\uff0c\u4ee5\u53ca\u4ece\u771f\u5b9e\u62a5\u9519\u4e2d\u627e\u5230\u6b63\u786e\u7684\u4fee\u590d\u4f4d\u7f6e\u3002\u672c\u6587\u8fd0\u884c\u7684\u662f cleanyerreads\uff0c\u4e0d\u628a\u8fd9\u6b21\u7ed3\u679c\u6269\u5c55\u6210\u5b8c\u6574\u7684\u7ec4\u88c5\u3001\u6ce8\u91ca\u6216\u8010\u836f\u5206\u6790\u6027\u80fd\u8bc4\u6d4b\u3002\u8bbe\u8ba1\u6570\u636e\u4e5f\u4e0d\u662f\u4e34\u5e8a\u6216\u73af\u5883\u6d4b\u5e8f\uff0c\u4e0d\u80fd\u636e\u6b64\u627f\u8bfa\u771f\u5b9e\u9879\u76ee\u7684\u51c6\u786e\u7387\u548c\u8fd0\u884c\u65f6\u95f4\u3002<\/p>\n

\"\u4efb\u52a1\u5b8c\u6210\uff0c\u4e0d\u7b49\u4e8e\u6837\u672c\u5408\u683c\u3002\u7528\u4e24\u4e2a\u8bbe\u8ba1\u6837\u672c\u68c0\u67e5\u8d28\u91cf\u72b6\u6001\u4e0e\u7f13\u5b58\u590d\u7528\u3002\u6d41\u7a0b\u7ed3\u679c\uff0c\u4e24\u6b21\u9000\u51fa\u7801\u5747\u4e3a\u96f6\uff0c\u4f4e\u6570\u636e\u91cf\u6837\u672c\u4ecd\u6709\u8d28\u91cf\u9519\u8bef\u3002\u8d28\u91cf\u68c0\u67e5\uff0c60,000
\u7528\u4e24\u4e2a\u8bbe\u8ba1\u6837\u672c\u68c0\u67e5\u8d28\u91cf\u72b6\u6001\u4e0e\u7f13\u5b58\u590d\u7528<\/figcaption><\/figure>\n

\u4e00\u3001\u5148\u660e\u786e\u5de5\u5177\u66ff\u4f60\u7ba1\u7406\u4e86\u4ec0\u4e48<\/h2>\n

Bactopia \u628a\u7ec6\u83cc\u57fa\u56e0\u7ec4\u5206\u6790\u4e2d\u7684\u8f93\u5165\u6574\u7406\u3001\u8f6f\u4ef6\u8c03\u7528\u3001\u7ed3\u679c\u7ec4\u7ec7\u548c\u4efb\u52a1\u8c03\u5ea6\u653e\u8fdb\u7edf\u4e00\u6d41\u7a0b\u3002\u5bf9\u4e8e\u62e5\u6709\u591a\u4e2a\u6837\u672c\u7684\u9879\u76ee\uff0c\u5b83\u7684\u4e3b\u8981\u4ef7\u503c\u662f\u51cf\u5c11\u6bcf\u4e2a\u6837\u672c\u5404\u5199\u4e00\u4efd\u811a\u672c\u9020\u6210\u7684\u8def\u5f84\u9519\u8bef\u3001\u53c2\u6570\u6f02\u79fb\u548c\u7ed3\u679c\u76ee\u5f55\u6df7\u4e71\u3002\u5e95\u5c42\u5de5\u5177\u7684\u5224\u65ad\u89c4\u5219\u4ecd\u7136\u5b58\u5728\uff0c\u7814\u7a76\u8005\u4f9d\u65e7\u8981\u89e3\u91ca\u6837\u672c\u91cf\u3001\u8bfb\u6bb5\u8d28\u91cf\u3001\u8986\u76d6\u6df1\u5ea6\u548c\u540e\u7eed\u5206\u6790\u7684\u9002\u7528\u6761\u4ef6\u3002\u7edf\u4e00\u5165\u53e3\u80fd\u63d0\u9ad8\u4e00\u81f4\u6027\uff0c\u5374\u4e0d\u80fd\u66ff\u4ee3\u5bf9\u8f93\u5165\u548c\u8f93\u51fa\u7684\u5224\u65ad\u3002<\/p>\n

\u5b98\u65b9\u63d0\u4f9b\u591a\u79cd\u5de5\u4f5c\u6d41\u3002\u9009\u62e9\u4e4b\u524d\uff0c\u5e94\u5148\u5199\u4e0b\u5f53\u524d\u95ee\u9898\u9700\u8981\u54ea\u4e00\u6b65\u7ed3\u679c\u3002\u5982\u679c\u53ea\u60f3\u68c0\u67e5\u6570\u636e\u662f\u5426\u9002\u5408\u7ee7\u7eed\u5206\u6790\uff0c\u5148\u8fd0\u884c\u8d28\u63a7\u5c31\u662f\u5408\u7406\u7684\u5c0f\u5b9e\u9a8c\u3002\u76f4\u63a5\u628a\u6240\u6709\u53ef\u9009\u6d41\u7a0b\u90fd\u6253\u5f00\uff0c\u4f1a\u8ba9\u5931\u8d25\u539f\u56e0\u548c\u751f\u7269\u5b66\u95ee\u9898\u7ea0\u7f20\u5728\u4e00\u8d77\u3002\u8fd9\u91cc\u6309\u5b98\u65b9 cleanyerreads \u8bf4\u660e<\/a>\u9009\u62e9\u8d28\u63a7\u5165\u53e3\uff0c\u540c\u65f6\u4ee5\u672c\u6b21\u5b89\u88c5\u7248\u672c\u7684\u5e2e\u52a9\u8f93\u51fa\u6838\u5bf9\u53c2\u6570\u540d\u79f0\u3002<\/p>\n

\u8fd9\u79cd\u96c6\u6210\u5de5\u5177\u4e5f\u6709\u4ee3\u4ef7\u3002\u8fd0\u884c\u73af\u5883\u4e0d\u53ea\u5305\u542b\u4e00\u4e2a\u53ef\u6267\u884c\u6587\u4ef6\uff0c\u8fd8\u5305\u62ec Nextflow\u3001\u63d2\u4ef6\u3001\u5bb9\u5668\u955c\u50cf\u548c\u53ef\u80fd\u8bbf\u95ee\u7684\u7f51\u7edc\u8d44\u6e90\u3002\u5b89\u88c5\u4e3b\u7a0b\u5e8f\u5b8c\u6210\uff0c\u53ea\u4ee3\u8868\u4f9d\u8d56\u51c6\u5907\u7684\u4e00\u90e8\u5206\u3002\u9996\u6b21\u8fd0\u884c\u5361\u4f4f\u65f6\uff0c\u5e94\u5148\u8bc6\u522b\u5f53\u524d\u505c\u5728\u4e0b\u8f7d\u3001\u914d\u7f6e\u89e3\u6790\u8fd8\u662f\u5b9e\u9645\u5206\u6790\uff0c\u4e0d\u80fd\u628a\u4efb\u4f55\u7b49\u5f85\u90fd\u89e3\u91ca\u4e3a\u6570\u636e\u592a\u5927\u3002<\/p>\n

\u4e8c\u3001\u5b9e\u9a8c\u600e\u6837\u8bbe\u8ba1\uff0c\u54ea\u4e9b\u7ed3\u8bba\u4e0d\u80fd\u5916\u63a8<\/h2>\n

\u672c\u6b21\u4f7f\u7528 Bactopia 4.1.0\u3001Nextflow 26.04.6 \u548c nf-bactopia 2.1.7\u3002\u4e3b\u7a0b\u5e8f\u5bb9\u5668\u548c\u5b9e\u9645\u6267\u884c\u7684\u4e09\u4e2a\u4f9d\u8d56\u955c\u50cf\u90fd\u8bb0\u5f55\u4e86\u6458\u8981\u3002\u8d28\u63a7\u7ed3\u679c\u663e\u793a fastp \u4e3a 1.3.1\u3002\u955c\u50cf\u6807\u7b7e\u65b9\u4fbf\u9605\u8bfb\uff0c\u6458\u8981\u624d\u7528\u4e8e\u6838\u5bf9\u5177\u4f53\u955c\u50cf\u5185\u5bb9\u3002\u5de5\u4f5c\u6d41\u989d\u5916\u4e0b\u8f7d\u8fc7\u82e5\u5e72\u4f9d\u8d56\uff0c\u4f46\u4efb\u52a1\u8bb0\u5f55\u6ca1\u6709\u663e\u793a\u5b83\u4eec\u53c2\u4e0e\u672c\u6b21\u8ba1\u7b97\uff0c\u56e0\u6b64\u4e0d\u80fd\u628a\u4e0b\u8f7d\u6e05\u5355\u76f4\u63a5\u5199\u6210\u5b9e\u9645\u8fd0\u884c\u7684\u8f6f\u4ef6\u6e05\u5355\u3002<\/p>\n

\u8f93\u5165\u6e90\u662f\u516c\u5f00\u7684\u5927\u80a0\u6746\u83cc K-12 \u53c2\u8003\u5e8f\u5217 NC_000913.3\u3002\u811a\u672c\u68c0\u67e5\u5e8f\u5217\u6821\u9a8c\u503c\u540e\uff0c\u53d6\u524d\u4e8c\u5341\u4e07\u78b1\u57fa\u4f5c\u4e3a\u6559\u5b66\u7247\u6bb5\uff0c\u518d\u6309\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u751f\u6210\u957f\u5ea6\u4e3a\u4e00\u767e\u4e94\u5341\u78b1\u57fa\u7684\u53cc\u7aef\u8bfb\u6bb5\u3002\u7247\u6bb5\u957f\u5ea6\u8bbe\u5728\u4e09\u767e\u4e94\u5341\u81f3\u4e94\u767e\u4e94\u5341\u78b1\u57fa\u4e4b\u95f4\uff0c\u4e0d\u4eba\u4e3a\u52a0\u5165\u6d4b\u5e8f\u9519\u8bef\u6216\u63a5\u5934\u3002\u5145\u8db3\u7ec4\u751f\u6210\u4e09\u4e07\u5bf9\uff0c\u4f4e\u6570\u636e\u91cf\u7ec4\u53ea\u751f\u6210\u4e00\u767e\u5bf9\u3002\u4e24\u7ec4\u5dee\u522b\u6e05\u695a\uff0c\u9002\u5408\u89c2\u5bdf\u8d28\u91cf\u6761\u4ef6\u5982\u4f55\u89e6\u53d1\u3002<\/p>\n

\u8fd9\u91cc\u5c06\u9884\u671f\u57fa\u56e0\u7ec4\u5927\u5c0f\u586b\u5199\u4e3a\u4e8c\u5341\u4e07\uff0c\u662f\u56e0\u4e3a\u5b9e\u9a8c\u53ea\u56f4\u7ed5\u8fd9\u4e2a\u8bbe\u8ba1\u7247\u6bb5\u8ba1\u7b97\u6df1\u5ea6\u3002\u771f\u5b9e\u5927\u80a0\u6746\u83cc\u9879\u76ee\u4e0d\u80fd\u7167\u6284\u8fd9\u4e2a\u6570\u5b57\u3002\u82e5\u628a\u771f\u5b9e\u57fa\u56e0\u7ec4\u957f\u5ea6\u9519\u8bef\u5730\u586b\u5c0f\uff0c\u4f30\u7b97\u6df1\u5ea6\u4f1a\u88ab\u62ac\u9ad8\uff0c\u6837\u672c\u53ef\u80fd\u770b\u8d77\u6765\u6bd4\u5b9e\u9645\u66f4\u5145\u5206\u3002\u5e94\u4f9d\u636e\u9879\u76ee\u5bf9\u8c61\u548c\u53ef\u9760\u5148\u9a8c\u9009\u62e9\u957f\u5ea6\uff0c\u5e76\u660e\u786e\u5b83\u662f\u53c2\u8003\u957f\u5ea6\u3001\u4f30\u7b97\u957f\u5ea6\u8fd8\u662f\u7ec4\u88c5\u957f\u5ea6\u3002<\/p>\n

\u5408\u6210\u6570\u636e\u4e5f\u6709\u660e\u663e\u8fb9\u754c\u3002\u6240\u6709\u78b1\u57fa\u8d28\u91cf\u88ab\u8bbe\u4e3a\u9ad8\u503c\uff0c\u6ca1\u6709\u6a21\u62df\u6c61\u67d3\u3001\u6df7\u5408\u83cc\u3001\u6587\u5e93\u504f\u501a\u548c\u771f\u5b9e\u9519\u8bef\u5206\u5e03\u3002\u5b83\u9002\u5408\u68c0\u67e5\u6d41\u7a0b\u884c\u4e3a\u548c\u8ba1\u91cf\u5355\u4f4d\uff0c\u4e0d\u9002\u5408\u8bc4\u4ef7\u771f\u5b9e\u6837\u672c\u7684\u6e05\u6d17\u51c6\u786e\u7387\uff0c\u66f4\u4e0d\u80fd\u7528\u5b83\u5f97\u51fa\u67d0\u79cd\u6d4b\u5e8f\u5e73\u53f0\u7684\u666e\u904d\u8d28\u63a7\u9608\u503c\u3002<\/p>\n

\u4e09\u3001\u628a\u6837\u672c\u6e05\u5355\u5f53\u4f5c\u5206\u6790\u8f93\u5165\u7684\u4e00\u90e8\u5206<\/h2>\n

\u672c\u6b21\u6837\u672c\u6e05\u5355\u5305\u542b sample\u3001runtype\u3001genome_size\u3001species\u3001r1\u3001r2\u3001se\u3001ont\u3001assembly \u7b49\u5217\uff0c\u53cc\u7aef\u8f93\u5165\u4f7f\u7528 paired-end\u3002\u6837\u672c\u540d\u4e0d\u4ec5\u51fa\u73b0\u5728\u62a5\u544a\u4e2d\uff0c\u8fd8\u4f1a\u53c2\u4e0e\u8f93\u51fa\u8def\u5f84\u7ec4\u7ec7\uff0c\u56e0\u6b64\u5e94\u4fdd\u6301\u552f\u4e00\u3001\u7a33\u5b9a\uff0c\u5e76\u907f\u514d\u5728\u5206\u6790\u4e2d\u9014\u66f4\u540d\u3002\u53cc\u7aef\u6587\u4ef6\u7684\u5bf9\u5e94\u5173\u7cfb\u5c24\u5176\u503c\u5f97\u5355\u72ec\u68c0\u67e5\uff0c\u6587\u4ef6\u5b58\u5728\u5e76\u4e0d\u610f\u5473\u7740\u4e24\u4e2a\u6587\u4ef6\u6765\u81ea\u540c\u4e00\u6837\u672c\u3002<\/p>\n

\u521d\u59cb\u51c6\u5907\u9636\u6bb5\u5b9e\u9645\u8fd0\u884c\u8fc7 bactopia prepare\uff0c\u5e76\u68c0\u67e5\u4e86\u5b83\u751f\u6210\u7684\u6e05\u5355\u7ed3\u6784\u3002\u968f\u540e\u91cd\u590d\u51c6\u5907\u65f6\uff0c\u8f85\u52a9\u547d\u4ee4\u8bbf\u95ee\u5728\u7ebf\u57fa\u56e0\u7ec4\u5927\u5c0f\u8d44\u6599\u53d1\u751f\u7b49\u5f85\u3002\u9488\u5bf9\u8fd9\u4e2a\u5df2\u7ecf\u660e\u786e\u7269\u79cd\u5b57\u6bb5\u548c\u8bbe\u8ba1\u957f\u5ea6\u7684\u5c0f\u5b9e\u9a8c\uff0c\u6700\u7ec8\u590d\u73b0\u811a\u672c\u76f4\u63a5\u5199\u51fa\u76f8\u540c\u7ed3\u6784\u7684\u6e05\u5355\uff0c\u907f\u514d\u91cd\u590d\u7684\u5728\u7ebf\u67e5\u8be2\u3002\u8fd9\u4e2a\u5904\u7406\u89e3\u51b3\u7684\u662f\u8f93\u5165\u5143\u6570\u636e\u5df2\u77e5\u60c5\u51b5\u4e0b\u7684\u73af\u5883\u4f9d\u8d56\uff0c\u4e0d\u662f\u5bf9\u672a\u77e5\u6837\u672c\u7701\u7565\u7269\u79cd\u5224\u65ad\u3002<\/p>\n

\u6587\u7ae0\u4f7f\u7528\u7684\u8fd0\u884c\u5165\u53e3\u5982\u4e0b\uff0c\u6e05\u5355\u3001\u53c2\u6570\u6587\u4ef6\u548c\u8d44\u6e90\u914d\u7f6e\u7684\u751f\u6210\u4ee3\u7801\u5747\u5305\u542b\u5728\u590d\u73b0\u6750\u6599\u4e2d\u3002\u4e0d\u540c\u7248\u672c\u793a\u4f8b\u53ef\u80fd\u51fa\u73b0\u4e0d\u540c\u53c2\u6570\u540d\u79f0\uff0c\u56e0\u6b64\u590d\u5236\u547d\u4ee4\u540e\u8fd8\u8981\u6267\u884c\u672c\u673a\u5e2e\u52a9\u6838\u5bf9\u3002\u672c\u6b21\u5b9e\u9645\u751f\u6548\u7684\u662f --samples<\/code>\u3002<\/p>\n

bactopia --wf cleanyerreads \\\r\n  -profile docker \\\r\n  -c limits.config \\\r\n  -params-file parameters.json \\\r\n  --samples samples.tsv \\\r\n  --outdir results \\\r\n  --max_cpus 2 \\\r\n  --max_memory 6.GB \\\r\n  --max_retry 1\r\n<\/code><\/pre>\n
\u4e0d\u8981\u53ea\u4fdd\u5b58\u8fd9\u6761\u547d\u4ee4\u3002\u6e05\u5355\u4e2d\u7684\u8def\u5f84\u3001\u4e24\u4e2a\u8bfb\u6bb5\u6587\u4ef6\u3001\u9884\u671f\u957f\u5ea6\u3001\u53c2\u6570\u6587\u4ef6\u3001\u73af\u5883\u7248\u672c\u548c\u8fd0\u884c\u65e5\u5fd7\u5171\u540c\u6784\u6210\u53ef\u590d\u67e5\u7684\u8f93\u5165\u3002\u53ea\u7559\u4e0b\u6700\u7ec8\u62a5\u544a\uff0c\u540e\u9762\u5f88\u96be\u5224\u65ad\u5dee\u5f02\u6765\u81ea\u65b0\u6570\u636e\u3001\u4e0d\u540c\u53c2\u6570\u8fd8\u662f\u8f6f\u4ef6\u73af\u5883\u53d8\u5316\u3002<\/p>\n
\u56db\u3001\u5148\u770b\u8bfb\u6570\uff0c\u518d\u770b\u78b1\u57fa\u6570\u548c\u6df1\u5ea6<\/h2>\n
\u5b9e\u9645\u7ed3\u679c\u5982\u4e0b\u3002\u8bfb\u6bb5\u6761\u6570\u5c06\u4e24\u7aef\u76f8\u52a0\uff0c\u4e0d\u80fd\u8bef\u5199\u6210\u8bfb\u6bb5\u5bf9\u6570\u3002\u8d28\u63a7\u524d\u7684\u4e5d\u767e\u4e07\u78b1\u57fa\u6765\u81ea\u516d\u4e07\u6761\u8bfb\u6bb5\uff0c\u800c\u4e0d\u662f\u516d\u4e07\u5bf9\u3002\u4f4e\u6570\u636e\u91cf\u7ec4\u4e24\u767e\u6761\u8bfb\u6bb5\u5bf9\u5e94\u4e00\u767e\u5bf9\u3002\u7edf\u8ba1\u8868\u82e5\u6ca1\u6709\u5199\u6e05\u5355\u4f4d\uff0c\u540e\u7eed\u8ba1\u7b97\u8986\u76d6\u6df1\u5ea6\u65f6\u5f88\u5bb9\u6613\u51fa\u73b0\u4e24\u500d\u8bef\u5dee\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n\n\n\n
\u68c0\u67e5\u9879<\/th>\n\u6570\u636e\u91cf\u5145\u8db3\u7ec4<\/th>\n\u4f4e\u6570\u636e\u91cf\u7ec4<\/th>\n<\/tr>\n<\/thead>\n
\u8f93\u5165\u8bfb\u6bb5\u5bf9\u6570<\/td>\n30,000<\/td>\n100<\/td>\n<\/tr>\n
\u53cc\u7aef\u5408\u8ba1\u8bfb\u6570<\/td>\n60,000<\/td>\n200<\/td>\n<\/tr>\n
\u8f93\u5165\u78b1\u57fa\u603b\u6570<\/td>\n9,000,000<\/td>\n30,000<\/td>\n<\/tr>\n
\u8d28\u63a7\u540e\u78b1\u57fa\u603b\u6570<\/td>\n8,998,900<\/td>\n30,000<\/td>\n<\/tr>\n
\u53cc\u7aef\u5408\u8ba1\u4f30\u7b97\u6df1\u5ea6<\/td>\n\u7ea6 44.9945 \u500d<\/td>\n0.15 \u500d<\/td>\n<\/tr>\n
\u8f93\u51fa\u8bfb\u6bb5\u7684\u7c7b\u522b<\/td>\n\u666e\u901a\u6e05\u6d01\u8bfb\u6bb5<\/td>\nerror-fastq \u6587\u4ef6<\/td>\n<\/tr>\n
\u521d\u6b21\u76f8\u5173\u4efb\u52a1\u72b6\u6001<\/td>\nCOMPLETED<\/td>\nCOMPLETED<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u5145\u8db3\u7ec4\u6e05\u6d17\u540e\u4ecd\u6709\u516d\u4e07\u6761\u8bfb\u6bb5\uff0c\u4f46\u5c11\u4e86\u4e00\u5343\u4e00\u767e\u4e2a\u78b1\u57fa\u3002\u539f\u59cb fastp \u62a5\u544a\u5c06\u8fd9\u90e8\u5206\u5f52\u5165\u63a5\u5934\u526a\u5207\uff0c\u5171\u6d89\u53ca\u4e8c\u5341\u56db\u6761\u8bfb\u6bb5\u3002\u7531\u4e8e\u751f\u6210\u811a\u672c\u6ca1\u6709\u52a0\u5165\u63a5\u5934\uff0c\u8fd9\u4e2a\u7ed3\u679c\u63d0\u9192\u6211\u4eec\uff0c\u5de5\u5177\u7684\u5206\u7c7b\u540d\u79f0\u63cf\u8ff0\u7684\u662f\u7b97\u6cd5\u91c7\u53d6\u7684\u64cd\u4f5c\uff0c\u4e0d\u80fd\u81ea\u52a8\u5f53\u4f5c\u8f93\u5165\u771f\u5b9e\u5b58\u5728\u63a5\u5934\u7684\u72ec\u7acb\u8bc1\u636e\u3002\u672c\u6b21\u6ca1\u6709\u9010\u4e00\u5206\u6790\u8fd9\u4e9b\u5c40\u90e8\u5e8f\u5217\u4e3a\u4f55\u89e6\u53d1\u526a\u5207\uff0c\u4e5f\u6ca1\u6709\u5f00\u5c55\u66ff\u4ee3\u53c2\u6570\u5bf9\u7167\uff0c\u56e0\u6b64\u4e0d\u80fd\u8fdb\u4e00\u6b65\u5ba3\u5e03\u67d0\u4e2a\u7b97\u6cd5\u673a\u5236\u5df2\u7ecf\u88ab\u786e\u8ba4\u3002<\/p>\n
\u6b63\u786e\u505a\u6cd5\u662f\u4fdd\u7559\u8fd9\u6761\u5f02\u5e38\u7ebf\u7d22\uff0c\u628a\u526a\u5207\u524d\u540e\u7684\u5e8f\u5217\u3001\u62a5\u544a\u4e2d\u7684\u63a5\u5934\u8ba1\u6570\u548c\u5177\u4f53\u7248\u672c\u653e\u5728\u4e00\u8d77\u590d\u67e5\u3002\u9ad8\u8d28\u91cf\u5206\u6570\u5e76\u4e0d\u4fdd\u8bc1\u7b97\u6cd5\u5b8c\u5168\u4e0d\u6539\u52a8\u8bfb\u6bb5\uff0c\u540c\u6837\uff0c\u51fa\u73b0\u5c11\u91cf\u526a\u5207\u4e5f\u4e0d\u80fd\u76f4\u63a5\u5224\u65ad\u6574\u6279\u6570\u636e\u6709\u95ee\u9898\u3002\u5148\u6838\u5bf9\u6570\u91cf\u7ea7\u548c\u5f71\u54cd\u8303\u56f4\uff0c\u518d\u51b3\u5b9a\u662f\u5426\u9700\u8981\u6269\u5927\u6392\u67e5\u3002<\/p>\n
\u5145\u8db3\u7ec4\u4e24\u4e2a\u5355\u7aef\u7edf\u8ba1\u7684\u6700\u7ec8\u6df1\u5ea6\u5404\u7ea6\u4e3a\u4e8c\u5341\u4e8c\u70b9\u56db\u4e5d\u4e03\u4e8c\u500d\uff0c\u4e24\u7aef\u5408\u8ba1\u624d\u7ea6\u4e3a\u56db\u5341\u56db\u70b9\u4e5d\u4e5d\u56db\u4e94\u500d\u3002\u8fd9\u662f\u78b1\u57fa\u603b\u91cf\u9664\u4ee5\u8bbe\u8ba1\u7247\u6bb5\u957f\u5ea6\u5f97\u5230\u7684\u5e73\u5747\u91cf\uff0c\u4e0d\u662f\u901a\u8fc7\u6bd4\u5bf9\u5f97\u5230\u7684\u6bcf\u4e2a\u4f4d\u70b9\u6df1\u5ea6\u3002\u5373\u4f7f\u5e73\u5747\u503c\u5408\u9002\uff0c\u771f\u5b9e\u6837\u672c\u4ecd\u53ef\u80fd\u6709\u4f4e\u8986\u76d6\u533a\u3001\u6c61\u67d3\u8bfb\u6bb5\u6216\u4e0d\u5747\u5300\u8986\u76d6\uff0c\u56e0\u6b64\u4e0d\u80fd\u7701\u7565\u540e\u7eed\u6309\u7814\u7a76\u95ee\u9898\u8fdb\u884c\u7684\u6bd4\u5bf9\u548c\u8986\u76d6\u68c0\u67e5\u3002<\/p>\n
\u4e94\u3001\u6700\u5bb9\u6613\u8bef\u5224\u7684\u5730\u65b9\u662f\u8d28\u91cf\u5931\u8d25\u4ecd\u53ef\u6b63\u5e38\u7ed3\u675f<\/h2>\n
\u4f4e\u6570\u636e\u91cf\u7ec4\u5728\u8f93\u5165\u6574\u7406\u9636\u6bb5\u4ea7\u751f\u4f4e\u8bfb\u6570\u548c\u4f4e\u78b1\u57fa\u91cf\u9519\u8bef\u6587\u4ef6\u3002\u9519\u8bef\u8bf4\u660e\u663e\u793a\uff0c\u4e24\u767e\u6761\u603b\u8bfb\u6570\u4f4e\u4e8e\u4e03\u5343\u56db\u767e\u4e03\u5341\u4e8c\u6761\u6761\u4ef6\uff0c\u4e09\u4e07\u78b1\u57fa\u4f4e\u4e8e\u4e8c\u767e\u4e8c\u5341\u56db\u4e07\u4e00\u5343\u516b\u767e\u4e8c\u5341\u78b1\u57fa\u6761\u4ef6\u3002\u5230\u4e86\u8d28\u63a7\u9636\u6bb5\uff0c\u8fd8\u80fd\u770b\u5230\u4f4e\u8986\u76d6\u6761\u4ef6\u5bf9\u5e94\u7684\u9519\u8bef\u6587\u4ef6\uff0c\u660e\u786e\u63d0\u5230\u4e8c\u767e\u4e07\u78b1\u57fa\u4e0e\u5341\u500d\u8986\u76d6\u8981\u6c42\u3002\u8fd9\u91cc\u8bb0\u5f55\u7684\u662f\u672c\u6b21\u8fd0\u884c\u7684\u5b9e\u9645\u89e6\u53d1\u6761\u4ef6\uff0c\u4e0d\u5e94\u628a\u5b83\u4eec\u6284\u6210\u6240\u6709\u9879\u76ee\u7684\u56fa\u5b9a\u8d28\u91cf\u6807\u51c6\u3002<\/p>\n
\u9700\u8981\u7279\u522b\u7ea0\u6b63\u4e00\u79cd\u8fc7\u5ea6\u7b80\u5316\u7684\u8bf4\u6cd5\u3002\u8fd9\u4e2a\u6837\u672c\u5e76\u6ca1\u6709\u5728\u8f93\u5165\u6574\u7406\u540e\u5b8c\u5168\u505c\u6b62\u4e00\u5207\u5904\u7406\u3002\u4efb\u52a1\u8bb0\u5f55\u663e\u793a\u5b83\u4ecd\u7136\u6267\u884c\u4e86\u8d28\u63a7\uff0c\u751f\u6210\u4e86\u8d28\u91cf\u7edf\u8ba1\u548c\u5e26\u9519\u8bef\u540e\u7f00\u7684\u8bfb\u6bb5\u6587\u4ef6\u3002\u5b83\u7684\u4efb\u52a1\u72b6\u6001\u4e5f\u662f\u5b8c\u6210\uff0c\u4e0d\u80fd\u56e0\u4e3a\u72b6\u6001\u680f\u662f\u7eff\u8272\uff0c\u5c31\u5c06\u5b83\u548c\u666e\u901a\u6e05\u6d01\u8f93\u51fa\u4e00\u8d77\u65e0\u6761\u4ef6\u9001\u5f80\u4e0b\u6e38\u3002<\/p>\n
\u81f3\u5c11\u5e94\u533a\u5206\u4e09\u5c42\u72b6\u6001\u3002\u7b2c\u4e00\u5c42\u662f\u6574\u4e2a\u5de5\u4f5c\u6d41\u662f\u5426\u6b63\u5e38\u7ed3\u675f\uff0c\u7b2c\u4e8c\u5c42\u662f\u67d0\u4e2a\u8ba1\u7b97\u4efb\u52a1\u662f\u5426\u6210\u529f\u6267\u884c\uff0c\u7b2c\u4e09\u5c42\u662f\u6837\u672c\u662f\u5426\u6ee1\u8db3\u5f53\u524d\u8d28\u91cf\u6761\u4ef6\u3002\u524d\u4e24\u5c42\u4e3b\u8981\u53cd\u6620\u7a0b\u5e8f\u6267\u884c\uff0c\u7b2c\u4e09\u5c42\u624d\u4e0e\u6837\u672c\u80fd\u5426\u7ee7\u7eed\u5206\u6790\u76f4\u63a5\u76f8\u5173\u3002\u628a\u8fd9\u4e09\u5c42\u5408\u5e76\u6210\u4e00\u4e2a\u201c\u6210\u529f\u201d\u5b57\u6bb5\uff0c\u4f1a\u9690\u85cf\u6700\u5173\u952e\u7684\u8d28\u91cf\u4fe1\u606f\u3002<\/p>\n
\u6279\u91cf\u9879\u76ee\u53ef\u4ee5\u636e\u6b64\u8bbe\u8ba1\u4ea4\u4ed8\u8868\uff0c\u6bcf\u4e2a\u6837\u672c\u540c\u65f6\u8bb0\u5f55\u4efb\u52a1\u72b6\u6001\u3001\u8d28\u91cf\u9519\u8bef\u7c7b\u522b\u3001\u6709\u6548\u8bfb\u6570\u3001\u4f30\u7b97\u6df1\u5ea6\u4ee5\u53ca\u662f\u5426\u8fdb\u5165\u4e0b\u4e00\u6b65\u3002\u5bf9\u5931\u8d25\u6837\u672c\u4fdd\u7559\u539f\u59cb\u8bfb\u6bb5\u548c\u539f\u56e0\uff0c\u4e0d\u8981\u901a\u8fc7\u964d\u4f4e\u6761\u4ef6\u6765\u8ba9\u5168\u8868\u53d8\u7eff\u3002\u786e\u5b9e\u9700\u8981\u8c03\u6574\u9608\u503c\u65f6\uff0c\u5e94\u7ed9\u51fa\u5bf9\u8c61\u5dee\u5f02\u6216\u5206\u6790\u76ee\u6807\u7684\u4f9d\u636e\uff0c\u5e76\u8bb0\u5f55\u8c03\u6574\u540e\u5bf9\u7ed3\u8bba\u8303\u56f4\u7684\u5f71\u54cd\u3002<\/p>\n
\u516d\u3001\u6062\u590d\u8fd0\u884c\u8981\u770b\u4efb\u52a1\u8bb0\u5f55\uff0c\u4e0d\u80fd\u53ea\u770b\u547d\u4ee4<\/h2>\n
\u7b2c\u4e00\u6b21\u6b63\u5f0f\u8fd0\u884c\u5171\u6709\u4e94\u4e2a\u4efb\u52a1\uff0c\u5747\u663e\u793a COMPLETED\uff0c\u5305\u62ec\u4e24\u4e2a\u6837\u672c\u7684\u8f93\u5165\u6574\u7406\u3001\u4e24\u4e2a\u6837\u672c\u7684\u8d28\u63a7\u4ee5\u53ca\u4e00\u4e2a\u6c47\u603b\u4efb\u52a1\u3002\u968f\u540e\u4fdd\u6301\u8f93\u5165\u6587\u4ef6\u3001\u53c2\u6570\u548c\u5de5\u4f5c\u76ee\u5f55\u4e0d\u53d8\uff0c\u8ffd\u52a0 -resume<\/code> \u518d\u6b21\u6267\u884c\u3002\u7b2c\u4e8c\u6b21\u8bb0\u5f55\u4e2d\uff0c\u540c\u6837\u4e94\u4e2a\u4efb\u52a1\u5168\u90e8\u663e\u793a CACHED\u3002\u8fd9\u624d\u662f\u672c\u6b21\u786e\u8ba4\u7f13\u5b58\u590d\u7528\u7684\u76f4\u63a5\u8bc1\u636e\u3002<\/p>\n
bactopia --wf cleanyerreads \\\r\n  -profile docker \\\r\n  -c limits.config \\\r\n  -params-file parameters.json \\\r\n  --samples samples.tsv \\\r\n  --outdir results \\\r\n  --max_cpus 2 \\\r\n  --max_memory 6.GB \\\r\n  --max_retry 1 \\\r\n  -resume\r\n<\/code><\/pre>\n
\u6062\u590d\u8fd0\u884c\u5e76\u4e0d\u662f\u53ea\u8981\u7ed3\u679c\u76ee\u5f55\u5b58\u5728\u5c31\u80fd\u751f\u6548\u3002\u8fd0\u884c\u7f13\u5b58\u3001\u5de5\u4f5c\u76ee\u5f55\u4e2d\u7684\u4efb\u52a1\u4ea7\u7269\u548c\u53ef\u8bc6\u522b\u7684\u8f93\u5165\u5171\u540c\u5f71\u54cd\u80fd\u5426\u590d\u7528\u3002\u82e5\u5c06\u8bfb\u6bb5\u91cd\u65b0\u538b\u7f29\u3001\u6362\u8def\u5f84\u3001\u6539\u53c2\u6570\u6216\u6e05\u7406\u5de5\u4f5c\u76ee\u5f55\uff0c\u4e0d\u80fd\u9884\u8bbe\u4ecd\u7136\u5168\u90e8\u547d\u4e2d\u3002\u590d\u73b0\u811a\u672c\u5728\u4e00\u6b21\u751f\u6210\u6570\u636e\u540e\u7acb\u5373\u6267\u884c\u4e24\u8f6e\u547d\u4ee4\uff0c\u907f\u514d\u5728\u4e24\u8f6e\u4e4b\u95f4\u91cd\u65b0\u751f\u6210\u538b\u7f29\u8f93\u5165\uff0c\u8fd9\u662f\u4e3a\u4e86\u8ba9\u5bf9\u7167\u6761\u4ef6\u4fdd\u6301\u6e05\u6670\u3002<\/p>\n
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Bactopia \u9002\u5408\u9700\u8981\u7edf\u4e00\u7ba1\u7406\u591a\u4e2a\u7ec6\u83cc\u6837\u672c\u3001\u5e0c\u671b\u4fdd\u5b58\u53c2\u6570\u548c\u8fd0\u884c\u8f68\u8ff9\u7684\u5206\u6790\u5de5\u4f5c\u3002\u5b83\u7684\u4f18\u52bf\u5728\u4e8e\u7ec4\u7ec7\u8fc7\u7a0b\uff0c\u4fbf\u4e8e\u628a\u76f8\u540c\u89c4\u5219\u5e94\u7528\u5230\u6574\u6279\u6570\u636e\uff0c\u5e76\u5229\u7528\u4efb\u52a1\u7f13\u5b58\u51cf\u5c11\u91cd\u590d\u8ba1\u7b97\u3002\u5c40\u9650\u662f\u4f9d\u8d56\u5c42\u6b21\u8f83\u591a\uff0c\u76ee\u5f55\u548c\u72b6\u6001\u8bed\u4e49\u9700\u8981\u5b66\u4e60\uff0c\u800c\u4e14\u6d41\u7a0b\u9ed8\u8ba4\u9009\u62e9\u4e0d\u4e00\u5b9a\u9002\u5408\u7279\u6b8a\u7269\u79cd\u3001\u5f02\u5e38\u6587\u5e93\u6216\u975e\u5e38\u89c4\u8f93\u5165\u3002<\/p>\n
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