{"id":72976,"date":"2026-11-15T13:01:50","date_gmt":"2026-11-15T05:01:50","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72976"},"modified":"2026-11-15T13:01:50","modified_gmt":"2026-11-15T05:01:50","slug":"genomad-short-contig-filter-calibration-fdr-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/genomad-short-contig-filter-calibration-fdr-practice\/","title":{"rendered":"geNomad \u5b9e\u8dd1\u6559\u7a0b\uff5c500 bp \u7247\u6bb5\u4e3a\u4f55\u5148\u88ab\u4fdd\u7559\u53c8\u88ab\u8fc7\u6ee4\uff0c\u600e\u6837\u8bfb\u61c2\u5206\u6570\u3001\u6821\u51c6\u4e0e\u5b8c\u6574\u5ea6"},"content":{"rendered":"
\n

\u8bc6\u522b\u5019\u9009\u75c5\u6bd2\u65f6\uff0c\u6700\u5bb9\u6613\u4ea7\u751f\u8bef\u89e3\u7684\u662f\u4e00\u4e32\u63a5\u8fd1\u4e00\u7684\u5206\u6570\u3002\u5b83\u770b\u8d77\u6765\u50cf\u662f\u7ed9\u51fa\u4e86\u660e\u786e\u7b54\u6848\uff0c\u5374\u6ca1\u6709\u81ea\u52a8\u56de\u7b54\u5e8f\u5217\u662f\u5426\u5b8c\u6574\u3001\u5c5e\u4e8e\u54ea\u4e2a\u7269\u79cd\u3001\u8fb9\u754c\u662f\u5426\u51c6\u786e\uff0c\u4ee5\u53ca\u8fd9\u4e2a\u6570\u503c\u5728\u5f53\u524d\u6837\u672c\u80cc\u666f\u4e0b\u5e94\u8be5\u600e\u6837\u89e3\u91ca\u3002geNomad \u63d0\u4f9b\u6807\u8bb0\u3001\u5e8f\u5217\u6a21\u578b\u3001\u6c47\u603b\u8fc7\u6ee4\u548c\u53ef\u9009\u5206\u6570\u6821\u51c6\uff0c\u771f\u6b63\u8bfb\u61c2\u7ed3\u679c\u9700\u8981\u628a\u8fd9\u4e9b\u73af\u8282\u5206\u522b\u6838\u5bf9\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u5b89\u88c5 geNomad 1.13.0\uff0c\u4f7f\u7528\u6570\u636e\u5e93 1.9\uff0c\u56f4\u7ed5\u516c\u5f00\u7684\u566c\u83cc\u4f53 lambda \u53c2\u8003\u5e8f\u5217\u53ca\u5176\u4e0d\u540c\u957f\u5ea6\u7247\u6bb5\u505a\u4e86\u5bf9\u7167\u3002\u6700\u6709\u610f\u601d\u7684\u7ed3\u679c\u662f\uff0c\u4e94\u767e\u78b1\u57fa\u7247\u6bb5\u5728\u9ed8\u8ba4\u8fc7\u6ee4\u4e0b\u88ab\u4fdd\u7559\uff0c\u5207\u6362\u4fdd\u5b88\u8fc7\u6ee4\u540e\u88ab\u6392\u9664\uff0c\u800c\u5b83\u7684\u6821\u51c6\u5206\u6570\u5e76\u6ca1\u6709\u6539\u53d8\u3002\u8fd9\u4e2a\u53d8\u5316\u6765\u81ea\u540e\u5904\u7406\u6761\u4ef6\uff0c\u4e0d\u662f\u6a21\u578b\u7a81\u7136\u8ba4\u4e3a\u540c\u4e00\u6761\u5e8f\u5217\u53d8\u6210\u4e86\u53e6\u4e00\u79cd\u5bf9\u8c61\u3002<\/p>\n

\"\u5206\u6570
500 bp \u7247\u6bb5\u7684\u6807\u8bb0\u5bcc\u96c6\u503c\u89e3\u91ca\u4e86\u53bb\u7559<\/figcaption><\/figure>\n

\u4e00\u3001\u5de5\u5177\u89e3\u51b3\u7684\u6838\u5fc3\u95ee\u9898\u662f\u4ec0\u4e48<\/h2>\n

geNomad \u7528\u4e8e\u4ece\u6838\u9178\u5e8f\u5217\u4e2d\u8bc6\u522b\u75c5\u6bd2\u548c\u8d28\u7c92\u5019\u9009\uff0c\u5e76\u63d0\u4f9b\u57fa\u56e0\u6807\u8bb0\u548c\u5206\u7c7b\u4fe1\u606f\u3002\u5176\u6d41\u7a0b\u540c\u65f6\u5229\u7528\u6807\u8bb0\u8bc1\u636e\u548c\u5e8f\u5217\u6a21\u578b\uff0c\u518d\u6574\u5408\u7ed3\u679c\u3002\u5bf9\u8bfb\u8005\u6765\u8bf4\uff0c\u91cd\u8981\u7684\u4e0d\u662f\u8bb0\u4f4f\u6240\u6709\u6a21\u5757\u540d\u79f0\uff0c\u800c\u662f\u77e5\u9053\u4e00\u4e2a\u6700\u7ec8\u5019\u9009\u53ef\u80fd\u7ecf\u5386\u4e86\u57fa\u56e0\u9884\u6d4b\u3001\u6807\u8bb0\u641c\u7d22\u3001\u6a21\u578b\u8bc4\u5206\u548c\u6c47\u603b\u8fc7\u6ee4\uff0c\u4e0d\u80fd\u628a\u6700\u7ec8\u8868\u683c\u770b\u6210\u5355\u6b21\u76f8\u4f3c\u6027\u641c\u7d22\u7684\u76f4\u63a5\u8f93\u51fa\u3002\u5b98\u65b9\u6d41\u7a0b\u8bf4\u660e<\/a><\/p>\n

\u5b83\u9002\u5408\u5728\u7ec4\u88c5\u540e\u5bf9\u5927\u91cf\u5e8f\u5217\u8fdb\u884c\u7b5b\u9009\uff0c\u4e5f\u9002\u5408\u68c0\u67e5\u67d0\u6761\u5019\u9009\u5e8f\u5217\u5177\u6709\u4ec0\u4e48\u5206\u7c7b\u8bc1\u636e\u3002\u4f18\u52bf\u662f\u4e0d\u540c\u7c7b\u578b\u7684\u4fe1\u606f\u53ef\u4ee5\u5728\u7edf\u4e00\u7ed3\u679c\u4e2d\u67e5\u770b\u3002\u5c40\u9650\u662f\u77ed\u5e8f\u5217\u3001\u964c\u751f\u7c7b\u7fa4\u548c\u590d\u6742\u7684\u5bbf\u4e3b\u7247\u6bb5\u4ecd\u7136\u9700\u8981\u590d\u6838\uff0c\u4efb\u4f55\u5355\u4e2a\u5206\u6570\u90fd\u65e0\u6cd5\u66ff\u4ee3\u540e\u7eed\u7684\u8d28\u91cf\u548c\u8fb9\u754c\u68c0\u67e5\u3002<\/p>\n

\u56e0\u6b64\uff0c\u5206\u6790\u76ee\u6807\u5e94\u5148\u5199\u5177\u4f53\u3002\u5bfb\u627e\u66f4\u591a\u5019\u9009\u4e0e\u5efa\u7acb\u8f83\u4fdd\u5b88\u7684\u5206\u6790\u96c6\u5408\uff0c\u53ef\u4ee5\u4f7f\u7528\u4e0d\u540c\u7684\u8fc7\u6ee4\u89c4\u5219\uff0c\u4f46\u8fd9\u4e9b\u9009\u62e9\u5fc5\u987b\u5728\u62a5\u544a\u4e2d\u8bf4\u660e\u3002\u82e5\u4e24\u4e2a\u6837\u672c\u7528\u4e86\u4e0d\u540c\u89c4\u5219\uff0c\u6700\u540e\u53ea\u6bd4\u8f83\u75c5\u6bd2\u6761\u6570\uff0c\u5c31\u53ef\u80fd\u628a\u7b5b\u9009\u7b56\u7565\u7684\u5dee\u5f02\u8bef\u8ba4\u4e3a\u751f\u7269\u5b66\u5dee\u5f02\u3002<\/p>\n

\u4e8c\u3001\u5b9e\u9a8c\u8f93\u5165\u600e\u6837\u6784\u9020<\/h2>\n

\u8f93\u5165\u5305\u62ec\u4e03\u6761\u5e8f\u5217\u3002\u4e94\u6761\u6765\u81ea\u516c\u5f00\u7684 lambda \u53c2\u8003 NC_001416.1\uff0c\u5206\u522b\u662f\u5b8c\u6574\u5e8f\u5217\u3001\u524d\u534a\u6bb5\u3001\u524d\u4e94\u5343\u78b1\u57fa\u3001\u524d\u4e24\u5343\u78b1\u57fa\u548c\u524d\u4e94\u767e\u78b1\u57fa\u3002\u53e6\u5916\u4e24\u6761\u53d6\u81ea\u5927\u80a0\u6746\u83cc K-12 \u53c2\u8003 NC_000913.3 \u7684\u524d\u4e94\u4e07\u548c\u524d\u4e24\u5343\u78b1\u57fa\uff0c\u4f5c\u4e3a\u7ec6\u83cc\u6765\u6e90\u5bf9\u7167\u3002\u811a\u672c\u6838\u5bf9\u4e24\u4e2a\u53c2\u8003\u5e8f\u5217\u7684\u6821\u9a8c\u503c\uff0c\u518d\u6309\u56fa\u5b9a\u4f4d\u7f6e\u622a\u53d6\uff0c\u6ca1\u6709\u4fee\u6539\u78b1\u57fa\u6216\u62fc\u63a5\u65b0\u7684\u751f\u7269\u5e8f\u5217\u3002<\/p>\n

\u8fd9\u4e9b\u8f93\u5165\u6545\u610f\u8ba9\u5e8f\u5217\u6765\u6e90\u4fdd\u6301\u6e05\u695a\uff0c\u4f46\u5b83\u4eec\u4e0d\u662f\u72ec\u7acb\u91c7\u96c6\u7684\u4e03\u4e2a\u6837\u672c\u3002\u4e94\u6761\u566c\u83cc\u4f53\u5e8f\u5217\u5f7c\u6b64\u5305\u542b\uff0c\u4e0d\u5e94\u5f53\u4f5c\u4e94\u4e2a\u72ec\u7acb\u75c5\u6bd2\u79cd\uff0c\u4e5f\u4e0d\u80fd\u7528\u6765\u8ba1\u7b97\u9762\u5411\u81ea\u7136\u73af\u5883\u7684\u7075\u654f\u5ea6\u6216\u51c6\u786e\u7387\u3002\u672c\u5b9e\u9a8c\u56de\u7b54\u7684\u662f\u540c\u4e00\u6765\u6e90\u7684\u4e0d\u540c\u957f\u5ea6\u8f93\u5165\u600e\u6837\u7ecf\u8fc7\u8bc4\u5206\u4e0e\u8fc7\u6ee4\u3002<\/p>\n

\u5b8c\u6574 lambda \u8f93\u5165\u957f\u56db\u4e07\u516b\u5343\u4e94\u767e\u96f6\u4e8c\u78b1\u57fa\uff0c\u524d\u534a\u6bb5\u957f\u4e8c\u4e07\u56db\u5343\u4e8c\u767e\u4e94\u5341\u4e00\u78b1\u57fa\u3002\u5df2\u77e5\u622a\u53d6\u4f4d\u7f6e\u8ba9\u6211\u4eec\u80fd\u591f\u660e\u786e\u533a\u5206\u201c\u6765\u6e90\u4e8e\u75c5\u6bd2\u201d\u4e0e\u201c\u5305\u542b\u5b8c\u6574\u75c5\u6bd2\u57fa\u56e0\u7ec4\u201d\u3002\u4e94\u767e\u78b1\u57fa\u7247\u6bb5\u5373\u4f7f\u88ab\u6b63\u786e\u8bc6\u522b\u51fa\u75c5\u6bd2\u4fe1\u53f7\uff0c\u4e5f\u4ecd\u7136\u53ea\u662f\u53c2\u8003\u5e8f\u5217\u7684\u4e00\u5c0f\u90e8\u5206\u3002\u8fd9\u4e00\u70b9\u4e0d\u9700\u8981\u6a21\u578b\u8bc4\u5206\u6765\u51b3\u5b9a\u3002<\/p>\n

\u4e09\u3001\u5b89\u88c5\u548c\u6570\u636e\u5e93\u4e5f\u8981\u6838\u5bf9\u7248\u672c\u5173\u7cfb<\/h2>\n

\u672c\u6b21\u4f7f\u7528\u56fa\u5b9a\u6458\u8981\u7684\u5bb9\u5668\u5b89\u88c5\u7a0b\u5e8f\uff0c\u901a\u8fc7\u5b9e\u9645\u7248\u672c\u8f93\u51fa\u786e\u8ba4\u7248\u672c\u3002\u6570\u636e\u5e93\u4ece\u7ef4\u62a4\u8005\u63d0\u4f9b\u7684\u4e0b\u8f7d\u7ad9\u53d6\u5f97\uff0c\u538b\u7f29\u5305\u5927\u5c0f\u4e3a\u516b\u4ebf\u56db\u5343\u4e8c\u767e\u591a\u4e07\u5b57\u8282\uff0c\u5e76\u4e0e\u516c\u5f00\u8bb0\u5f55\u7684\u6821\u9a8c\u503c\u6838\u5bf9\u4e00\u81f4\u3002\u8f6f\u4ef6\u4e0e\u6570\u636e\u5e93\u4e4b\u95f4\u7684\u517c\u5bb9\u5173\u7cfb\u53e6\u67e5\u4e86\u7ef4\u62a4\u8005\u7684\u53d1\u5e03\u8868\uff0c\u786e\u8ba4\u6570\u636e\u5e93 1.9 \u5bf9\u5e94\u672c\u6b21\u7a0b\u5e8f\u7248\u672c\u3002\u7248\u672c\u5bf9\u5e94\u8868<\/a><\/p>\n

\u4e0b\u8f7d\u65f6\u6700\u521d\u4f7f\u7528\u7684\u955c\u50cf\u8bbf\u95ee\u8f83\u6162\uff0c\u4e4b\u540e\u5207\u6362\u5230\u7ef4\u62a4\u8005\u4e0b\u8f7d\u7ad9\u3002\u91c7\u7528\u5206\u6bb5\u4f20\u8f93\u540e\uff0c\u4ecd\u7136\u5bf9\u5b8c\u6574\u6587\u4ef6\u91cd\u65b0\u8ba1\u7b97\u6821\u9a8c\u503c\u3002\u6570\u636e\u5e93\u538b\u7f29\u5305\u5185\u90e8\u5305\u542b\u6307\u5411\u672c\u76ee\u5f55\u5176\u4ed6\u6587\u4ef6\u7684\u94fe\u63a5\uff0c\u89e3\u538b\u524d\u6838\u5bf9\u4e86\u76ee\u6807\u8303\u56f4\u3002\u8fd9\u4e9b\u68c0\u67e5\u4fdd\u8bc1\u6211\u4eec\u8fd0\u884c\u7684\u662f\u5b8c\u6574\u4e14\u53ef\u8ffd\u8e2a\u7684\u6570\u636e\u5e93\uff0c\u4e0d\u80fd\u53ea\u51ed\u76ee\u5f55\u5df2\u7ecf\u51fa\u73b0\u5c31\u8ba4\u5b9a\u5b89\u88c5\u5b8c\u6210\u3002<\/p>\n

\u6b63\u5f0f\u8fd0\u884c\u9650\u5b9a\u4e24\u4e2a\u7ebf\u7a0b\uff0c\u5e76\u5c06\u6807\u8bb0\u641c\u7d22\u62c6\u6210\u516b\u4efd\uff0c\u4ee5\u63a7\u5236\u672c\u6b21\u5b9e\u9a8c\u7684\u5185\u5b58\u4f7f\u7528\u3002\u62c6\u5206\u6570\u636e\u5e93\u5f71\u54cd\u7684\u662f\u641c\u7d22\u7ec4\u7ec7\u65b9\u5f0f\uff0c\u672c\u6b21\u6ca1\u6709\u901a\u8fc7\u964d\u4f4e\u641c\u7d22\u7075\u654f\u5ea6\u6765\u7f29\u77ed\u5b9e\u9a8c\u3002\u5b98\u65b9\u4e5f\u4ecb\u7ecd\u4e86\u62c6\u5206\u53c2\u6570\u7684\u7528\u9014\uff0c\u4f46\u5177\u4f53\u53d6\u503c\u4ecd\u9700\u8981\u7ed3\u5408\u5b9e\u9645\u73af\u5883\u9a8c\u8bc1\u3002\u5e38\u89c1\u8fd0\u884c\u95ee\u9898<\/a><\/p>\n

genomad end-to-end \\\r\n  --threads 2 \\\r\n  --splits 8 \\\r\n  --enable-score-calibration \\\r\n  inputs.fna genomad_output genomad_db\r\n<\/code><\/pre>\n
\u6ce8\u610f\u8fd9\u91cc\u660e\u786e\u5f00\u542f\u4e86\u53ef\u9009\u5206\u6570\u6821\u51c6\u3002\u540e\u6587\u6240\u8bf4\u7684\u201c\u9ed8\u8ba4\u8fc7\u6ee4\u201d\u662f\u6307\u6c47\u603b\u9636\u6bb5\u91c7\u7528\u9ed8\u8ba4\u8fc7\u6ee4\u6761\u4ef6\uff0c\u4e0d\u8868\u793a\u6574\u6761\u547d\u4ee4\u5b8c\u5168\u6ca1\u6709\u4fee\u6539\u9ed8\u8ba4\u9009\u9879\u3002\u51c6\u786e\u63cf\u8ff0\u8fd9\u79cd\u533a\u522b\uff0c\u6bd4\u7b3c\u7edf\u5199\u201c\u4f7f\u7528\u9ed8\u8ba4\u53c2\u6570\u201d\u66f4\u5bb9\u6613\u8ba9\u8bfb\u8005\u590d\u73b0\u7ed3\u679c\u3002<\/p>\n
\u56db\u3001\u5148\u8bfb\u5230\u4ec0\u4e48\uff0c\u518d\u89e3\u91ca\u4e3a\u4ec0\u4e48<\/h2>\n
\u9ed8\u8ba4\u8fc7\u6ee4\u5f97\u5230\u4e94\u6761\u75c5\u6bd2\u5019\u9009\uff0c\u6b63\u597d\u662f\u4e94\u6761 lambda \u6765\u6e90\u8f93\u5165\uff0c\u4e24\u6761\u7ec6\u83cc\u5bf9\u7167\u6ca1\u6709\u8fdb\u5165\u6700\u7ec8\u75c5\u6bd2\u6216\u8d28\u7c92\u96c6\u5408\u3002\u5bbd\u677e\u8fc7\u6ee4\u4ecd\u7136\u5f97\u5230\u8fd9\u4e94\u6761\uff0c\u4fdd\u5b88\u8fc7\u6ee4\u5219\u53ea\u4fdd\u7559\u5176\u4e2d\u56db\u6761\u3002\u6240\u6709\u6c47\u603b\u547d\u4ee4\u90fd\u6b63\u5e38\u7ed3\u675f\uff0c\u6ca1\u6709\u901a\u8fc7\u4fee\u6539\u8f93\u5165\u5185\u5bb9\u5236\u9020\u8fd9\u4e9b\u5dee\u5f02\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n\n
lambda \u8f93\u5165\u957f\u5ea6<\/th>\n\u9884\u6d4b\u57fa\u56e0\u6570<\/th>\n\u6821\u51c6\u75c5\u6bd2\u5206\u6570<\/th>\n\u9ed8\u8ba4 \/ \u4fdd\u5b88\u4fdd\u7559<\/th>\n<\/tr>\n<\/thead>\n
48,502 bp<\/td>\n62<\/td>\n0.9985<\/td>\n\u662f \/ \u662f<\/td>\n<\/tr>\n
24,251 bp<\/td>\n25<\/td>\n0.9988<\/td>\n\u662f \/ \u662f<\/td>\n<\/tr>\n
5,000 bp<\/td>\n5<\/td>\n0.9987<\/td>\n\u662f \/ \u662f<\/td>\n<\/tr>\n
2,000 bp<\/td>\n2<\/td>\n0.9955<\/td>\n\u662f \/ \u662f<\/td>\n<\/tr>\n
500 bp<\/td>\n1<\/td>\n0.8846<\/td>\n\u662f \/ \u5426<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u534a\u957f\u7247\u6bb5\u7684\u5206\u6570\u751a\u81f3\u7565\u9ad8\u4e8e\u5b8c\u6574\u5e8f\u5217\u3002\u8fd9\u5e76\u4e0d\u5947\u602a\uff0c\u56e0\u4e3a\u5206\u7c7b\u5206\u6570\u4e0e\u5e8f\u5217\u8986\u76d6\u4e86\u5b8c\u6574\u53c2\u8003\u7684\u591a\u5c11\u4e0d\u662f\u540c\u4e00\u4e2a\u91cf\u3002\u4e0d\u80fd\u7528\u4e24\u8005\u7684\u5c0f\u6570\u5dee\u522b\u7ed9\u57fa\u56e0\u7ec4\u5b8c\u6574\u5ea6\u6392\u5e8f\uff0c\u4e5f\u4e0d\u80fd\u56e0\u4e3a\u534a\u957f\u7247\u6bb5\u5206\u6570\u66f4\u9ad8\uff0c\u5c31\u8ba4\u4e3a\u5b83\u6bd4\u5b8c\u6574\u8f93\u5165\u66f4\u9002\u5408\u4ee3\u8868\u6574\u4e2a\u57fa\u56e0\u7ec4\u3002<\/p>\n
\u4e94\u767e\u78b1\u57fa\u7247\u6bb5\u88ab\u9884\u6d4b\u51fa\u4e00\u4e2a\u57fa\u56e0\uff0c\u5e76\u5177\u6709\u4e00\u4e2a\u75c5\u6bd2\u6807\u5fd7\u57fa\u56e0\u547d\u4e2d\u3002\u77ed\u5e8f\u5217\u5e76\u4e0d\u662f\u4ec5\u51ed\u957f\u5ea6\u5c31\u81ea\u52a8\u5168\u90e8\u5220\u9664\uff0c\u5b9e\u9645\u662f\u5426\u4fdd\u7559\u8fd8\u53d7\u5176\u4ed6\u8bc1\u636e\u5f71\u54cd\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u672c\u6b21\u77ed\u7247\u6bb5\u6765\u81ea\u5df2\u77e5\u53c2\u8003\uff0c\u5177\u5907\u76f8\u5e94\u6807\u8bb0\uff0c\u4e0d\u4ee3\u8868\u6240\u6709\u4e94\u767e\u78b1\u57fa\u73af\u5883\u7247\u6bb5\u90fd\u540c\u6837\u5bb9\u6613\u5206\u7c7b\u3002<\/p>\n
\u4e94\u3001\u5206\u6570\u8d85\u8fc7\u9608\u503c\uff0c\u4ecd\u7136\u53ef\u80fd\u88ab\u5176\u4ed6\u6761\u4ef6\u8fc7\u6ee4<\/h2>\n
\u4e94\u767e\u78b1\u57fa\u7247\u6bb5\u7684\u6821\u51c6\u75c5\u6bd2\u5206\u6570\u4e3a\u96f6\u70b9\u516b\u516b\u56db\u516d\uff0c\u8d85\u8fc7\u4fdd\u5b88\u8fc7\u6ee4\u7684\u5206\u6570\u95e8\u69db\uff0c\u4f46\u5b83\u4ecd\u88ab\u6392\u9664\u3002\u67e5\u770b\u7ed3\u679c\u540e\u53d1\u73b0\uff0c\u5176\u75c5\u6bd2\u6807\u8bb0\u5bcc\u96c6\u503c\u4e3a\u4e00\u70b9\u4e09\u4e5d\u4e03\u516d\uff0c\u4f4e\u4e8e\u4fdd\u5b88\u9884\u8bbe\u8981\u6c42\u7684\u4e00\u70b9\u4e94\u3002\u8fd9\u8bf4\u660e\u6700\u7ec8\u5019\u9009\u96c6\u5408\u7531\u4e00\u7ec4\u6761\u4ef6\u5171\u540c\u51b3\u5b9a\uff0c\u4e0d\u80fd\u53ea\u62ff\u5206\u6570\u5217\u53bb\u89e3\u91ca\u6240\u6709\u4fdd\u7559\u548c\u6392\u9664\u7ed3\u679c\u3002\u540e\u5904\u7406\u8fc7\u6ee4\u8bf4\u660e<\/a><\/p>\n
\u4e3a\u786e\u8ba4\u8fd9\u4e2a\u89e3\u91ca\uff0c\u672c\u6b21\u53c8\u505a\u4e86\u4e00\u8f6e\u53ea\u8c03\u6574\u75c5\u6bd2\u6807\u8bb0\u5bcc\u96c6\u6761\u4ef6\u7684\u6c47\u603b\u3002\u5176\u4f59\u6761\u4ef6\u56de\u5230\u9ed8\u8ba4\u503c\uff0c\u53ea\u628a\u5bcc\u96c6\u9608\u503c\u8bbe\u4e3a\u4e00\u70b9\u4e94\uff0c\u7ed3\u679c\u540c\u6837\u53ea\u5269\u56db\u6761\uff0c\u6392\u9664\u7684\u4ecd\u662f\u4e94\u767e\u78b1\u57fa\u7247\u6bb5\u3002\u8fd9\u4e2a\u5355\u6761\u4ef6\u5bf9\u7167\u6bd4\u4ec5\u51ed\u9884\u8bbe\u540d\u79f0\u63a8\u6d4b\u539f\u56e0\u66f4\u53ef\u9760\u3002<\/p>\n
genomad summary --conservative \\\r\n  inputs.fna genomad_output\r\n\r\ngenomad summary --min-virus-marker-enrichment 1.5 \\\r\n  inputs.fna genomad_output\r\n<\/code><\/pre>\n
\u6c47\u603b\u547d\u4ee4\u4f1a\u6539\u5199\u76f8\u5e94\u8f93\u51fa\uff0c\u56e0\u6b64\u5b9e\u8dd1\u811a\u672c\u5728\u6bcf\u6b21\u8fd0\u884c\u540e\u5206\u522b\u4fdd\u5b58\u4e86\u7ed3\u679c\u5feb\u7167\u3002\u6b63\u5f0f\u9879\u76ee\u91cc\u4e5f\u5e94\u4fdd\u7559\u4e0d\u540c\u7b5b\u9009\u89c4\u5219\u5bf9\u5e94\u7684\u8868\u683c\uff0c\u5426\u5219\u540e\u4e00\u6b21\u8fd0\u884c\u8986\u76d6\u524d\u4e00\u6b21\u7ed3\u679c\u540e\uff0c\u53ea\u5269\u4e00\u4e2a\u6587\u4ef6\u540d\uff0c\u8bfb\u8005\u5c06\u65e0\u6cd5\u8ffd\u8e2a\u5019\u9009\u6570\u91cf\u4e3a\u4ec0\u4e48\u53d8\u5316\u3002<\/p>\n
\u5bbd\u677e\u8fc7\u6ee4\u6ca1\u6709\u65b0\u589e\u5019\u9009\uff0c\u540c\u6837\u503c\u5f97\u4fdd\u7559\u3002\u5b83\u53ea\u662f\u5728\u8fd9\u7ec4\u8f93\u5165\u4e0a\u6ca1\u6709\u6539\u53d8\u6700\u7ec8\u96c6\u5408\uff0c\u4e0d\u80fd\u63a8\u5bfc\u4e3a\u5bbd\u677e\u53c2\u6570\u6c38\u8fdc\u6ca1\u6709\u5f71\u54cd\u3002\u6bd4\u8f83\u53c2\u6570\u65f6\uff0c\u4e0d\u5fc5\u4e3a\u4e86\u8ba9\u6587\u7ae0\u770b\u8d77\u6765\u6709\u53d8\u5316\u5c31\u7ee7\u7eed\u8c03\u5230\u51fa\u73b0\u60f3\u8981\u7684\u7ed3\u679c\u3002\u6ca1\u6709\u5dee\u5f02\u4e5f\u662f\u4e00\u4e2a\u6709\u660e\u786e\u9002\u7528\u8303\u56f4\u7684\u89c2\u5bdf\u3002<\/p>\n
\u516d\u3001\u5c0f\u6837\u672c\u6821\u51c6\u51fa\u73b0\u4e86\u4e00\u4e2a\u7248\u672c\u654f\u611f\u7ec6\u8282<\/h2>\n
\u672c\u6b21\u8f93\u5165\u53ea\u6709\u4e03\u6761\u5e8f\u5217\uff0c\u5374\u5b9e\u9645\u751f\u6210\u4e86\u6821\u51c6\u7ed3\u679c\u548c\u975e\u7a7a\u7684\u9519\u8bef\u53d1\u73b0\u7387\u5217\u3002\u68c0\u67e5\u65e5\u5fd7\u540e\u53d1\u73b0\uff0c\u7a0b\u5e8f\u6ca1\u6709\u4ece\u8fd9\u4e03\u6761\u5e8f\u5217\u7cbe\u786e\u4f30\u8ba1\u6837\u672c\u7ec4\u6210\uff0c\u800c\u662f\u5728\u81ea\u52a8\u6a21\u5f0f\u4e0b\u56de\u9000\u5230 metagenome \u9884\u8bbe\u3002\u6574\u5408\u6a21\u578b\u5bf9\u5e94\u7684\u7ec4\u6210\u503c\u4e3a\u67d3\u8272\u4f53\u96f6\u70b9\u4e03\u4e8c\u3001\u8d28\u7c92\u96f6\u70b9\u4e00\u4e03\u3001\u75c5\u6bd2\u96f6\u70b9\u4e00\u4e00\u3002\u5b83\u4eec\u662f\u6240\u7528\u9884\u8bbe\uff0c\u4e0d\u662f\u4ece\u4e03\u6761\u8bbe\u8ba1\u5e8f\u5217\u6d4b\u5f97\u7684\u771f\u5b9e\u6bd4\u4f8b\u3002<\/p>\n
\u8fd9\u4e0e\u7f51\u9875\u6982\u8ff0\u4e2d\u201c\u5c0f\u4e8e\u4e00\u5343\u6761\u65f6\u81ea\u52a8\u5173\u95ed\u6821\u51c6\u201d\u7684\u8868\u8ff0\u4e0d\u540c\u3002\u672c\u6b21\u4ee5 1.13.0 \u7684\u5b9e\u8dd1\u65e5\u5fd7\u548c\u76f8\u540c\u7248\u672c\u6e90\u7801\u4e3a\u51c6\uff0c\u6e90\u7801\u660e\u786e\u5305\u542b\u5c0f\u6837\u672c\u56de\u9000\u5230\u9884\u8bbe\u7684\u5904\u7406\u3002\u56e0\u6b64\uff0c\u8bfb\u8005\u4e0d\u80fd\u53ea\u6839\u636e\u4e00\u6bb5\u901a\u7528\u6559\u7a0b\u63a8\u65ad\u5f53\u524d\u7248\u672c\u662f\u5426\u771f\u6b63\u6267\u884c\u4e86\u67d0\u4e00\u6b65\u30021.13.0 \u6821\u51c6\u6a21\u5757\u6e90\u7801<\/a><\/p>\n
\u8fd9\u4e2a\u5b9e\u9a8c\u5e76\u4e0d\u662f\u5efa\u8bae\u5c0f\u6570\u636e\u96c6\u4e00\u5f8b\u6253\u5f00\u6821\u51c6\u3002\u5b83\u7684\u4f5c\u7528\u662f\u66b4\u9732\u6821\u51c6\u4f9d\u8d56\u7684\u80cc\u666f\u5047\u8bbe\u3002\u6211\u4eec\u7684\u8f93\u5165\u6545\u610f\u5bcc\u542b\u540c\u4e00\u75c5\u6bd2\u7684\u91cd\u590d\u6765\u6e90\u7247\u6bb5\uff0c\u4e0e\u666e\u901a\u5b8f\u57fa\u56e0\u7ec4\u7ec4\u6210\u5e76\u4e0d\u76f8\u540c\uff0c\u56e0\u6b64\u4e0d\u80fd\u628a\u8fd9\u4e9b\u6821\u51c6\u5206\u6570\u5f53\u4f5c\u771f\u5b9e\u9879\u76ee\u51c6\u786e\u7387\u3002\u66f4\u4e0d\u5e94\u8be5\u4e3a\u4e86\u8d85\u8fc7\u6570\u91cf\u95e8\u69db\uff0c\u590d\u5236\u540c\u4e00\u6279\u5e8f\u5217\u51d1\u51fa\u4e00\u5343\u6761\u518d\u5ba3\u79f0\u83b7\u5f97\u4e86\u53ef\u9760\u7ec4\u6210\u4f30\u8ba1\u3002<\/p>\n
\u4f8b\u5982\uff0c\u4e94\u767e\u78b1\u57fa\u7247\u6bb5\u7684\u672a\u6821\u51c6\u6574\u5408\u5206\u6570\u4e3a\u96f6\u70b9\u4e03\u516b\u4e00\u4e03\uff0c\u6821\u51c6\u540e\u53d8\u4e3a\u96f6\u70b9\u516b\u516b\u56db\u516d\u3002\u53d8\u5316\u6765\u81ea\u8bc4\u5206\u5904\u7406\u548c\u80cc\u666f\u8bbe\u5b9a\uff0c\u6ca1\u6709\u7ed9\u8fd9\u6761\u7247\u6bb5\u589e\u52a0\u65b0\u7684\u57fa\u56e0\u6216\u65b0\u7684\u5e8f\u5217\u957f\u5ea6\u3002\u8ba8\u8bba\u7ed3\u679c\u65f6\uff0c\u5e94\u540c\u65f6\u4fdd\u5b58\u6821\u51c6\u524d\u540e\u5206\u6570\u3001\u91c7\u7528\u7684\u7ec4\u6210\u65b9\u5f0f\u548c\u76f8\u5173\u63d0\u793a\uff0c\u800c\u4e0d\u662f\u53ea\u4fdd\u7559\u770b\u8d77\u6765\u66f4\u9ad8\u7684\u6570\u5b57\u3002<\/p>\n
\u4e03\u3001\u9519\u8bef\u53d1\u73b0\u7387\u4e0d\u662f\u8fd9\u4e00\u884c\u5e8f\u5217\u51fa\u9519\u7684\u6982\u7387<\/h2>\n
\u9ed8\u8ba4\u8fc7\u6ee4\u8868\u4e2d\uff0c\u4e94\u767e\u78b1\u57fa\u7247\u6bb5\u6392\u5728\u7b2c\u4e94\u884c\uff0c\u5b83\u7684\u9519\u8bef\u53d1\u73b0\u7387\u663e\u793a\u4e3a\u96f6\u70b9\u96f6\u4e8c\u56db\u516b\u3002\u8fd9\u4e2a\u503c\u5e76\u4e0d\u662f\u4e00\u51cf\u53bb\u8be5\u884c\u75c5\u6bd2\u5206\u6570\u3002\u7528\u663e\u793a\u7684\u5206\u6570\u8ba1\u7b97\uff0c\u4e00\u51cf\u53bb\u96f6\u70b9\u516b\u516b\u56db\u516d\u7ea6\u4e3a\u96f6\u70b9\u4e00\u4e00\u4e94\u56db\uff0c\u4e24\u8005\u663e\u7136\u4e0d\u540c\u3002\u5dee\u522b\u6765\u81ea\u7edf\u8ba1\u5bf9\u8c61\uff0c\u4e00\u4e2a\u6d89\u53ca\u5355\u6761\u5e8f\u5217\u7684\u6821\u51c6\u503c\uff0c\u53e6\u4e00\u4e2a\u6982\u62ec\u622a\u81f3\u8be5\u884c\u6240\u4fdd\u7559\u96c6\u5408\u7684\u9884\u8ba1\u9519\u8bef\u6bd4\u4f8b\u3002<\/p>\n
\u672c\u6b21\u524d\u4e94\u884c\u7684\u6821\u51c6\u5206\u6570\u5206\u522b\u7ea6\u4e3a\u96f6\u70b9\u4e5d\u4e5d\u516b\u516b\u3001\u96f6\u70b9\u4e5d\u4e5d\u516b\u4e03\u3001\u96f6\u70b9\u4e5d\u4e5d\u516b\u4e94\u3001\u96f6\u70b9\u4e5d\u4e5d\u4e94\u4e94\u548c\u96f6\u70b9\u516b\u516b\u56db\u516d\u3002\u5c06\u4e00\u51cf\u53bb\u5404\u5206\u6570\u7684\u503c\u52a0\u603b\uff0c\u518d\u9664\u4ee5\u4e94\uff0c\u7ea6\u4e3a\u96f6\u70b9\u96f6\u4e8c\u56db\u516b\uff0c\u4e0e\u8868\u683c\u4e00\u81f4\u3002\u56e0\u4e3a\u663e\u793a\u503c\u7ecf\u8fc7\u820d\u5165\uff0c\u624b\u5de5\u6838\u5bf9\u53ea\u5e94\u8981\u6c42\u5408\u7406\u7cbe\u5ea6\uff0c\u4e0d\u8981\u628a\u6700\u540e\u51e0\u4f4d\u5c0f\u6570\u7684\u5dee\u522b\u5f53\u4f5c\u7a0b\u5e8f\u9519\u8bef\u3002\u5206\u6570\u6821\u51c6\u4e0e\u9519\u8bef\u53d1\u73b0\u7387\u8bf4\u660e<\/a><\/p>\n
\u66f4\u91cd\u8981\u7684\u662f\uff0c\u8fd9\u4e2a\u4f30\u8ba1\u4ecd\u7136\u4f9d\u8d56\u6a21\u578b\u548c\u6821\u51c6\u80cc\u666f\uff0c\u4e0d\u80fd\u5728\u4e00\u4e2a\u4eba\u4e3a\u7ec4\u6210\u7684\u5c0f\u5b9e\u9a8c\u4e2d\u89c6\u4e3a\u7ecf\u8fc7\u72ec\u7acb\u9a8c\u8bc1\u7684\u9519\u8bef\u7387\u3002\u771f\u5b9e\u9879\u76ee\u82e5\u8981\u8bc4\u4ef7\u65b9\u6cd5\u8868\u73b0\uff0c\u5e94\u4f7f\u7528\u5408\u9002\u7684\u5df2\u77e5\u771f\u503c\u548c\u72ec\u7acb\u6d4b\u8bd5\u8bbe\u8ba1\uff0c\u800c\u4e0d\u662f\u628a\u5de5\u5177\u8f93\u51fa\u7684\u4f30\u8ba1\u503c\u518d\u62ff\u6765\u8bc1\u660e\u5de5\u5177\u672c\u8eab\u51c6\u786e\u3002<\/p>\n
\u516b\u3001\u5206\u7c7b\u4fe1\u606f\u3001\u62d3\u6251\u4e0e\u5b8c\u6574\u5ea6\u4e5f\u9700\u8981\u5206\u522b\u9605\u8bfb<\/h2>\n
\u672c\u6b21\u4e94\u6761\u75c5\u6bd2\u5019\u9009\u7684\u5e8f\u5217\u7ea7\u5206\u7c7b\u90fd\u505c\u5728 Caudoviricetes\uff0c\u540e\u7eed\u90e8\u5206\u5c42\u7ea7\u7559\u7a7a\u3002\u5373\u4fbf\u6211\u4eec\u77e5\u9053\u8f93\u5165\u6765\u81ea lambda\uff0c\u4e5f\u4e0d\u80fd\u636e\u6b64\u8981\u6c42\u7a0b\u5e8f\u7684\u6bcf\u4e2a\u5c42\u7ea7\u90fd\u5fc5\u987b\u586b\u5230\u7269\u79cd\u3002\u5de5\u5177\u6839\u636e\u6807\u8bb0\u652f\u6301\u7ed9\u51fa\u5e8f\u5217\u7ea7\u5206\u7c7b\uff0c\u67d0\u4e2a\u57fa\u56e0\u5177\u6709\u66f4\u5177\u4f53\u540d\u79f0\uff0c\u4e5f\u4e0d\u4ee3\u8868\u6574\u6761\u5e8f\u5217\u4e00\u5b9a\u5177\u6709\u8db3\u591f\u4e00\u81f4\u7684\u8bc1\u636e\u5230\u8fbe\u540c\u6837\u5c42\u7ea7\u3002\u5206\u7c7b\u5b66\u8d4b\u503c\u8bf4\u660e<\/a><\/p>\n
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