{"id":72978,"date":"2026-11-15T13:01:51","date_gmt":"2026-11-15T05:01:51","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72978"},"modified":"2026-11-15T13:04:36","modified_gmt":"2026-11-15T05:04:36","slug":"virsorter2-full-sequence-trimming-score-boundary-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/virsorter2-full-sequence-trimming-score-boundary-practice\/","title":{"rendered":"VirSorter2 \u5b9e\u8dd1\u6559\u7a0b\uff5c\u6807\u7740 full \u7684\u5e8f\u5217\u4e3a\u4ec0\u4e48\u53d8\u77ed\uff0c\u600e\u6837\u533a\u5206\u5019\u9009\u8bc6\u522b\u3001\u8fb9\u754c\u4e0e\u5b8c\u6574\u5ea6"},"content":{"rendered":"
\n

\u75c5\u6bd2\u8bc6\u522b\u7ed3\u679c\u4e2d\uff0c\u6700\u5bb9\u6613\u88ab\u8bef\u8bfb\u7684\u4e00\u4e2a\u540e\u7f00\u662f full\u3002\u8bfb\u8005\u53ef\u80fd\u628a\u5b83\u7406\u89e3\u4e3a\u5b8c\u6574\u75c5\u6bd2\u57fa\u56e0\u7ec4\uff0c\u4e5f\u53ef\u80fd\u8ba4\u4e3a\u8f93\u51fa\u4e00\u5b9a\u4fdd\u7559\u4e86\u8f93\u5165\u7684\u5168\u90e8\u78b1\u57fa\u3002\u5b9e\u9645\u8fd0\u884c VirSorter2 \u540e\uff0c\u6211\u4eec\u53d1\u73b0\u4e00\u6761\u8f93\u5165\u957f\u5ea6\u4e3a\u56db\u4e07\u516b\u5343\u4e94\u767e\u96f6\u4e8c\u7684\u5e8f\u5217\uff0c\u7ed3\u679c\u540d\u79f0\u4ecd\u5e26 full\uff0c\u9ed8\u8ba4\u8f93\u51fa\u5374\u53ea\u6709\u56db\u4e07\u4e03\u5343\u4e03\u767e\u4e94\u5341\u56db\u78b1\u57fa\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u5b89\u88c5 VirSorter2 2.2.4\uff0c\u4e0b\u8f7d\u5e76\u6838\u9a8c\u4f5c\u8005\u63d0\u4f9b\u7684\u6570\u636e\u5e93\uff0c\u5728\u516c\u5f00 lambda \u53c2\u8003\u5e8f\u5217\u53ca\u5176\u622a\u53d6\u7247\u6bb5\u4e0a\u5b8c\u6210\u56db\u7ec4\u8fd0\u884c\u3002\u9664\u4e86\u68c0\u67e5\u5206\u6570\u9608\u503c\uff0c\u8fd8\u5bf9\u6bd4\u4e86\u9ed8\u8ba4\u4fee\u526a\u4e0e\u4fdd\u7559\u539f\u5e8f\u5217\u7684\u8f93\u51fa\uff0c\u5e76\u9010\u6761\u6838\u5bf9\u8fb9\u754c\u5750\u6807\u548c\u5e8f\u5217\u6821\u9a8c\u503c\u3002\u5b9e\u9a8c\u76ee\u7684\u662f\u5e2e\u52a9\u8bfb\u8005\u6b63\u786e\u4f7f\u7528\u5019\u9009\u7ed3\u679c\uff0c\u4e0d\u662f\u8bc4\u4f30\u672a\u77e5\u75c5\u6bd2\u7684\u53d1\u73b0\u51c6\u786e\u7387\u3002<\/p>\n

\"\u6807\u7740
\u56db\u7ec4\u5b9e\u8dd1\uff0c\u6838\u5bf9\u5206\u6570\u3001\u8fb9\u754c\u4e0e\u5bfc\u51fa FASTA<\/figcaption><\/figure>\n

\u4e00\u3001\u5148\u628a\u5019\u9009\u8bc6\u522b\u3001\u5206\u7c7b\u548c\u5b8c\u6574\u5ea6\u5206\u6210\u4e09\u4e2a\u95ee\u9898<\/h2>\n

VirSorter2 \u901a\u8fc7\u57fa\u56e0\u548c\u76f8\u5173\u7279\u5f81\u8bc6\u522b\u5019\u9009\u75c5\u6bd2\u5e8f\u5217\uff0c\u7ed3\u679c\u91cc\u540c\u65f6\u7ed9\u51fa\u4e0d\u540c\u5206\u7c7b\u5668\u7684\u5206\u6570\u3002\u5b83\u9002\u5408\u63d0\u4f9b\u5019\u9009\u7b5b\u9009\u8bc1\u636e\uff0c\u4f46\u8fd9\u4e9b\u5206\u7c7b\u5668\u7684\u540d\u79f0\u4e0d\u80fd\u76f4\u63a5\u4f5c\u4e3a\u53ef\u9760\u7684\u5206\u7c7b\u5b66\u7ed3\u8bba\u3002\u4f5c\u8005\u4e5f\u660e\u786e\u8bf4\u660e\uff0c\u4e0d\u540c\u5206\u7c7b\u5668\u7684\u9002\u7528\u5e8f\u5217\u7a7a\u95f4\u53ef\u80fd\u91cd\u53e0\u3002\u9879\u76ee\u8bf4\u660e<\/a><\/p>\n

\u8bc6\u522b\u5019\u9009\u56de\u7b54\u7684\u662f\u201c\u8fd9\u6bb5\u5e8f\u5217\u662f\u5426\u5177\u6709\u75c5\u6bd2\u76f8\u5173\u4fe1\u53f7\u201d\u3002\u5206\u7c7b\u5b66\u5224\u65ad\u8fd8\u9700\u8981\u8003\u8651\u4e0d\u540c\u5c42\u7ea7\u7684\u8bc1\u636e\uff0c\u5b8c\u6574\u5ea6\u5219\u6d89\u53ca\u8f93\u5165\u8986\u76d6\u4e86\u591a\u5c11\u57fa\u56e0\u7ec4\u4ee5\u53ca\u662f\u5426\u5b58\u5728\u5bbf\u4e3b\u7247\u6bb5\u7b49\u95ee\u9898\u3002\u628a\u8fd9\u4e09\u4ef6\u4e8b\u90fd\u4ea4\u7ed9\u4e00\u4e2a\u6700\u9ad8\u5206\u5b57\u6bb5\uff0c\u4f1a\u8ba9\u540e\u7eed\u62a5\u544a\u8d85\u51fa\u5de5\u5177\u5b9e\u9645\u652f\u6301\u7684\u8303\u56f4\u3002<\/p>\n

\u5bf9\u4e8e\u8bfb\u8005\u6765\u8bf4\uff0c\u4e00\u4e2a\u5b9e\u7528\u4e60\u60ef\u662f\u4e3a\u6bcf\u6761\u91cd\u70b9\u5019\u9009\u4fdd\u7559\u4e0d\u540c\u95ee\u9898\u7684\u8bc1\u636e\u5217\u3002\u5019\u9009\u8bc6\u522b\u8bb0\u5f55\u6765\u81ea\u54ea\u4e2a\u5de5\u5177\u3001\u5206\u6570\u548c\u6807\u8bb0\u652f\u6301\uff0c\u8fb9\u754c\u8bb0\u5f55\u8f93\u5165\u4e0e\u8f93\u51fa\u4e4b\u95f4\u7684\u5bf9\u5e94\u5173\u7cfb\uff0c\u8d28\u91cf\u8bc4\u4f30\u8bb0\u5f55\u4e13\u95e8\u65b9\u6cd5\u7684\u7ed3\u8bba\u3002\u8fd9\u6837\u5373\u4f7f\u4ee5\u540e\u8c03\u6574\u67d0\u4e00\u73af\u8282\uff0c\u4e5f\u80fd\u77e5\u9053\u54ea\u4e9b\u5224\u65ad\u9700\u8981\u8ddf\u7740\u66f4\u65b0\u3002<\/p>\n

\u4e8c\u3001\u672c\u6b21\u8f93\u5165\u4e0e\u53c2\u6570\u6709\u4ec0\u4e48\u8fb9\u754c<\/h2>\n

\u8f93\u5165\u5305\u62ec\u516c\u5f00 lambda \u53c2\u8003 NC_001416.1 \u7684\u5168\u957f\u5e8f\u5217\uff0c\u4ee5\u53ca\u5b83\u7684\u524d\u4e8c\u4e07\u56db\u5343\u4e8c\u767e\u4e94\u5341\u4e00\u3001\u4e94\u5343\u3001\u4e24\u5343\u548c\u4e94\u767e\u78b1\u57fa\u3002\u53e6\u5916\u52a0\u5165\u516c\u5f00\u5927\u80a0\u6746\u83cc\u53c2\u8003\u7684\u524d\u4e94\u4e07\u4e0e\u4e24\u5343\u78b1\u57fa\uff0c\u5171\u4e03\u6761\u3002\u622a\u53d6\u7247\u6bb5\u6ca1\u6709\u4eba\u4e3a\u7a81\u53d8\uff0c\u4e5f\u6ca1\u6709\u62fc\u63a5\u6210\u5047\u60f3\u524d\u75c5\u6bd2\u3002<\/p>\n

\u7a0b\u5e8f\u8bbe\u7f6e\u6700\u77ed\u8f93\u5165\u957f\u5ea6\u4e3a\u4e00\u5343\u4e94\u767e\uff0c\u56e0\u6b64\u4e94\u767e\u78b1\u57fa\u7247\u6bb5\u5728\u9884\u5904\u7406\u5c31\u88ab\u79fb\u9664\u3002\u65e5\u5fd7\u660e\u786e\u8bb0\u5f55\u79fb\u9664\u4e00\u6761\u3001\u5269\u4f59\u516d\u6761\u7ebf\u6027\u5e8f\u5217\u3002\u8fd9\u4e0d\u662f\u8be5\u7247\u6bb5\u7ecf\u8fc7\u5206\u7c7b\u5668\u540e\u5f97\u5230\u4f4e\u5206\uff0c\u4e5f\u4e0d\u662f\u672c\u6587\u6d4b\u51fa\u4e86\u5de5\u5177\u5bf9\u4e94\u767e\u78b1\u57fa\u75c5\u6bd2\u7684\u8bc6\u522b\u80fd\u529b\u3002<\/p>\n

\u672c\u6b21\u53ea\u542f\u7528 dsDNAphage \u548c ssDNA \u4e24\u7ec4\u5206\u7c7b\u5668\uff0c\u4f7f\u7528\u4e24\u4e2a\u8ba1\u7b97\u7ebf\u7a0b\u3002\u9009\u62e9\u8303\u56f4\u9700\u8981\u4e0e\u7814\u7a76\u95ee\u9898\u4e00\u81f4\uff0c\u672c\u6587\u6ca1\u6709\u8fd0\u884c RNA\u3001NCLDV \u6216\u5176\u4ed6\u5206\u7c7b\u5668\uff0c\u56e0\u6b64\u4e0d\u80fd\u628a\u7ed3\u679c\u5199\u6210\u5bf9\u6240\u6709\u75c5\u6bd2\u7c7b\u578b\u7684\u5168\u9762\u6d4b\u8bd5\u3002<\/p>\n

\u56db\u7ec4\u5206\u522b\u4e3a\u666e\u901a\u7b5b\u9009\u3001\u9ad8\u7f6e\u4fe1\u5ea6\u7b5b\u9009\u3001\u6700\u4f4e\u5206\u63d0\u9ad8\u5230\u96f6\u70b9\u4e5d\u4e5d\u3001\u4fdd\u7559\u539f\u5e8f\u5217\u3002\u540e\u4e09\u7ec4\u590d\u7528\u5df2\u6709\u7279\u5f81\u8fdb\u5165 classify \u9636\u6bb5\uff0c\u5e76\u4f7f\u7528\u4e0d\u540c\u6807\u7b7e\u4fdd\u5b58\u8f93\u51fa\u3002\u8fd9\u6837\u65e2\u4fdd\u7559\u4e86\u5bf9\u7167\uff0c\u4e5f\u907f\u514d\u56e0\u4e3a\u65b0\u7ed3\u679c\u8986\u76d6\u65e7\u6587\u4ef6\u800c\u5931\u53bb\u5dee\u5f02\u8bb0\u5f55\u3002<\/p>\n

\u4e09\u3001\u5b89\u88c5\u6210\u529f\u4e0d\u53ea\u610f\u5473\u7740\u7248\u672c\u547d\u4ee4\u80fd\u6267\u884c<\/h2>\n

\u672c\u6b21\u5148\u56fa\u5b9a\u5bb9\u5668\u955c\u50cf\uff0c\u518d\u6309\u8be5\u5b89\u88c5\u5305\u63d0\u4f9b\u7684\u4f9d\u8d56\u6e05\u5355\u5efa\u7acb\u5206\u6790\u73af\u5883\u3002\u4e3b\u7a0b\u5e8f\u80fd\u591f\u8f93\u51fa\u7248\u672c\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u6bcf\u4e2a\u5b50\u6b65\u9aa4\u9700\u8981\u7684\u5e93\u90fd\u5df2\u7ecf\u51c6\u5907\u597d\u3002\u521d\u6b21\u8fd0\u884c\u5c31\u5728\u9884\u5904\u7406\u5904\u9047\u5230\u7f3a\u5c11 screed \u7684\u9519\u8bef\uff0c\u95ee\u9898\u51fa\u5728\u5b50\u6b65\u9aa4\u5b9e\u9645\u4f7f\u7528\u7684 Python \u73af\u5883\u3002<\/p>\n

\u6211\u4eec\u5df2\u7ecf\u5efa\u7acb\u4e86\u4f9d\u8d56\u73af\u5883\uff0c\u4f46\u5bb9\u5668\u7684\u542f\u52a8\u5165\u53e3\u91cd\u65b0\u8bbe\u7f6e\u4e86\u8def\u5f84\uff0c\u8986\u76d6\u4e86\u5916\u90e8\u4f20\u5165\u7684\u8bbe\u7f6e\u3002\u68c0\u67e5\u5b9e\u9645 PATH \u548c Python \u4f4d\u7f6e\u540e\uff0c\u6539\u4e3a\u660e\u786e\u6307\u5b9a\u542f\u52a8\u5165\u53e3\uff0c\u8ba9\u5206\u6790\u6b65\u9aa4\u4f7f\u7528\u5df2\u7ecf\u5b89\u88c5\u7684\u4f9d\u8d56\uff0c\u968f\u540e\u56db\u7ec4\u8fd0\u884c\u5747\u5b8c\u6210\u3002\u8fd9\u4e2a\u7ecf\u9a8c\u6bd4\u7b80\u5355\u5730\u53cd\u590d\u5b89\u88c5\u540c\u4e00\u4e2a\u5305\u66f4\u503c\u5f97\u4fdd\u7559\u3002<\/p>\n

\u6392\u67e5\u7c7b\u4f3c\u9519\u8bef\u65f6\uff0c\u53ef\u4ee5\u5148\u786e\u8ba4\u62a5\u9519\u53d1\u751f\u5728\u4e3b\u7a0b\u5e8f\u8fd8\u662f\u5b50\u6d41\u7a0b\uff0c\u518d\u67e5\u770b\u8be5\u6b65\u9aa4\u5b9e\u9645\u8c03\u7528\u7684\u89e3\u91ca\u5668\u3002\u4ea4\u4e92\u73af\u5883\u91cc\u80fd\u591f\u5bfc\u5165\u67d0\u4e2a\u5e93\uff0c\u5e76\u4e0d\u80fd\u4fdd\u8bc1\u4efb\u52a1\u542f\u52a8\u540e\u4ecd\u4f7f\u7528\u540c\u4e00\u73af\u5883\u3002\u5e94\u4ece\u5931\u8d25\u65e5\u5fd7\u4e2d\u7684\u5177\u4f53\u547d\u4ee4\u8ffd\u8e2a\uff0c\u800c\u4e0d\u662f\u53ea\u67e5\u770b\u5f53\u524d\u7ec8\u7aef\u7684\u73af\u5883\u540d\u79f0\u3002<\/p>\n

\u6570\u636e\u5e93\u4e0b\u8f7d\u4e5f\u9700\u8981\u6838\u9a8c\u3002\u672c\u6b21\u5b8c\u6574\u538b\u7f29\u5305\u7ea6\u5341\u4e94\u4ebf\u5b57\u8282\uff0c\u89e3\u538b\u540e\u660e\u663e\u66f4\u5927\uff0c\u4e0d\u80fd\u53ea\u6309\u4e0b\u8f7d\u5305\u5927\u5c0f\u4f30\u8ba1\u7a7a\u95f4\u3002\u5206\u6bb5\u4e0b\u8f7d\u5b8c\u6210\u540e\u6838\u5bf9\u603b\u957f\u5ea6\u3001\u538b\u7f29\u5305 SHA256\uff0c\u5e76\u5c06\u4e03\u4e2a\u4e3b\u8981\u6570\u636e\u5e93\u6587\u4ef6\u7684 MD5 \u4e0e\u4f5c\u8005\u5b89\u88c5\u89c4\u5219\u9010\u9879\u6bd4\u8f83\u3002\u5b9e\u9645\u6821\u9a8c\u503c\u8bb0\u5f55\u5728\u590d\u73b0\u5305\u4e2d\u3002<\/p>\n

\u4e0b\u8f7d\u94fe\u63a5\u5b58\u5728\u77ed\u65f6\u6709\u6548\u671f\uff0c\u6700\u521d\u91cd\u590d\u4f7f\u7528\u540c\u4e00\u8df3\u8f6c\u5730\u5740\u5bfc\u81f4\u540e\u7eed\u5206\u6bb5\u8fd4\u56de\u62d2\u7edd\u8bbf\u95ee\u3002\u66f4\u65b0\u4e0b\u8f7d\u8bf7\u6c42\u5e76\u4fdd\u7559\u5df2\u9a8c\u8bc1\u7247\u6bb5\u540e\u5b8c\u6210\u7eed\u4f20\u3002\u8fd9\u91cc\u6ca1\u6709\u6539\u53d8\u6570\u636e\u5e93\u5185\u5bb9\uff0c\u4e5f\u6ca1\u6709\u7528\u4e0d\u540c\u6765\u6e90\u7684\u5c0f\u538b\u7f29\u5305\u6821\u9a8c\u503c\u53bb\u6838\u5bf9\u8fd9\u4e2a\u5b8c\u6574\u6570\u636e\u5e93\u5305\u3002\u4f5c\u8005\u6570\u636e\u5e93\u5b89\u88c5\u89c4\u5219<\/a><\/p>\n

\u56db\u3001\u56db\u7ec4\u7ed3\u679c\u5e94\u8be5\u600e\u6837\u8bfb<\/h2>\n

\u666e\u901a\u7b5b\u9009\u5f97\u5230\u56db\u6761\u75c5\u6bd2\u5019\u9009\uff0c\u5747\u6765\u81ea\u957f\u5ea6\u81f3\u5c11\u4e24\u5343\u7684 lambda \u8f93\u5165\uff0c\u4e24\u6761\u7ec6\u83cc\u7247\u6bb5\u672a\u8fdb\u5165\u6700\u7ec8\u5019\u9009\u3002\u9ad8\u7f6e\u4fe1\u5ea6\u7b5b\u9009\u4ecd\u4fdd\u7559\u56db\u6761\uff0c\u63d0\u9ad8\u6700\u4f4e\u5206\u5230\u96f6\u70b9\u4e5d\u4e5d\u540e\u4fdd\u7559\u4e09\u6761\uff0c\u53bb\u6389\u7684\u662f\u4e24\u5343\u78b1\u57fa\u8f93\u5165\u5bf9\u5e94\u7684\u5019\u9009\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n
\u8f93\u5165\u7247\u6bb5<\/th>\n\u8f93\u51fa bp<\/th>\n\u5206\u6570<\/th>\n\u6807\u8bb0\u6570<\/th>\n\u22650.99<\/th>\n<\/tr>\n<\/thead>\n
\u5168\u957f<\/td>\n47754<\/td>\n1.000<\/td>\n21<\/td>\n\u4fdd\u7559<\/td>\n<\/tr>\n
\u524d\u534a\u6bb5<\/td>\n23728<\/td>\n0.993<\/td>\n19<\/td>\n\u4fdd\u7559<\/td>\n<\/tr>\n
\u524d 5000<\/td>\n4809<\/td>\n1.000<\/td>\n4<\/td>\n\u4fdd\u7559<\/td>\n<\/tr>\n
\u524d 2000<\/td>\n1810<\/td>\n0.980<\/td>\n1<\/td>\n\u6392\u9664<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u9ad8\u7f6e\u4fe1\u5ea6\u9009\u9879\u4e0e\u201c\u628a\u9608\u503c\u8bbe\u4e3a\u96f6\u70b9\u4e5d\u4e5d\u201d\u5e76\u4e0d\u662f\u540c\u4e00\u4e2a\u6761\u4ef6\u3002\u672c\u6b21\u5b89\u88c5\u5305\u5e2e\u52a9\u663e\u793a\uff0c\u9ad8\u7f6e\u4fe1\u5ea6\u4f9d\u636e\u6700\u9ad8\u5206\u81f3\u5c11\u96f6\u70b9\u4e5d\uff0c\u6216\u8005\u6700\u9ad8\u5206\u81f3\u5c11\u96f6\u70b9\u4e03\u4e14\u81f3\u5c11\u6709\u4e00\u4e2a\u6807\u8bb0\u57fa\u56e0\u3002\u56db\u6761\u5019\u9009\u90fd\u6ee1\u8db3\u524d\u4e00\u6761\uff0c\u56e0\u6b64\u5168\u90e8\u4fdd\u7559\u662f\u7b26\u5408\u89c4\u5219\u7684\u3002<\/p>\n

\u8fd9\u4e5f\u8bf4\u660e\uff0c\u7b5b\u9009\u6ca1\u6709\u6539\u53d8\u65f6\u5e94\u5148\u6838\u5bf9\u8f93\u5165\u662f\u5426\u672c\u6765\u5c31\u90fd\u6ee1\u8db3\u6761\u4ef6\u3002\u4e0d\u80fd\u56e0\u4e3a\u4e24\u5f20\u7ed3\u679c\u8868\u4e00\u6837\uff0c\u5c31\u65ad\u8a00\u9009\u9879\u5931\u6548\u3002\u76f8\u53cd\uff0c\u63d0\u9ad8\u9608\u503c\u540e\u5c11\u4e86\u4e00\u6761\uff0c\u4e5f\u4e0d\u4ee3\u8868\u5176\u4ed6\u5019\u9009\u7684\u751f\u7269\u5b66\u72b6\u6001\u53d1\u751f\u53d8\u5316\uff0c\u53ea\u662f\u5b83\u4eec\u4e0e\u65b0\u5224\u5b9a\u89c4\u5219\u7684\u5173\u7cfb\u4e0d\u540c\u3002<\/p>\n

\u8868\u91cc\u663e\u793a\u4e3a\u4e00\u7684\u5206\u6570\u662f\u5de5\u5177\u8f93\u51fa\u683c\u5f0f\u4e0b\u7684\u6a21\u578b\u7ed3\u679c\uff0c\u4e0d\u662f\u5bf9\u771f\u5b9e\u8eab\u4efd\u7edd\u5bf9\u65e0\u8bef\u7684\u627f\u8bfa\u3002\u672c\u6b21\u4f7f\u7528\u5df2\u77e5\u53c2\u8003\u548c\u91cd\u53e0\u7247\u6bb5\uff0c\u4e5f\u6ca1\u6709\u8db3\u591f\u7684\u72ec\u7acb\u6837\u672c\u6765\u4f30\u8ba1\u771f\u5b9e\u73af\u5883\u4e2d\u7684\u8bef\u5224\u7387\uff0c\u4e0d\u80fd\u7528\u56db\u6761\u547d\u4e2d\u53bb\u5305\u88c5\u4e00\u4e2a\u603b\u4f53\u51c6\u786e\u7387\u3002<\/p>\n

\u4e94\u3001full \u540e\u7f00\u4e0e\u5e8f\u5217\u957f\u5ea6\uff0c\u5fc5\u987b\u7528\u6587\u4ef6\u6838\u5bf9<\/h2>\n

\u56db\u6761\u9ed8\u8ba4\u5019\u9009\u7684\u540d\u79f0\u90fd\u4ee5 ||full<\/code> \u7ed3\u5c3e\uff0c\u4f46\u5b83\u4eec\u7684 FASTA \u957f\u5ea6\u5168\u90e8\u77ed\u4e8e\u8f93\u5165\u3002\u5168\u957f\u8f93\u5165\u5c11\u4e86\u4e03\u767e\u56db\u5341\u516b\u78b1\u57fa\uff0c\u524d\u534a\u6bb5\u5c11\u4e86\u4e94\u767e\u4e8c\u5341\u4e09\uff0c\u4e94\u5343\u7247\u6bb5\u5c11\u4e86\u4e00\u767e\u4e5d\u5341\u4e00\uff0c\u4e24\u5343\u7247\u6bb5\u5c11\u4e86\u4e00\u767e\u4e5d\u5341\u3002\u8fd9\u91cc\u7684 full \u8868\u793a\u5019\u9009\u5728\u6574\u4f53\u4e0a\u7684\u75c5\u6bd2\u4fe1\u53f7\u72b6\u6001\uff0c\u4e0d\u4fdd\u8bc1\u5b8c\u6574\u4fdd\u7559\u8f93\u5165\uff0c\u4e5f\u4e0d\u7b49\u4e8e\u5b8c\u6574\u57fa\u56e0\u7ec4\u3002<\/p>\n

\u4fdd\u7559\u539f\u5e8f\u5217\u7684\u5bf9\u7167\u4f7f\u7528 --keep-original-seq<\/code>\u3002\u5b9e\u9645\u8f93\u51fa\u957f\u5ea6\u5206\u522b\u6062\u590d\u5230 48502\u300124251\u30015000 \u548c 2000\uff0c\u5e8f\u5217\u6821\u9a8c\u503c\u9010\u6761\u4e0e\u8f93\u5165\u4e00\u81f4\u3002\u8fd9\u7ed9\u51fa\u6bd4\u201c\u6587\u4ef6\u770b\u8d77\u6765\u5dee\u4e0d\u591a\u201d\u66f4\u660e\u786e\u7684\u8bc1\u636e\uff0c\u8bf4\u660e\u672c\u6b21\u8be5\u9009\u9879\u786e\u5b9e\u6539\u53d8\u4e86\u5bfc\u51fa\u7684 FASTA \u5185\u5bb9\u3002<\/p>\n

virsorter run -w result -i inputs.fna -d db \\\r\n  --min-length 1500 --include-groups dsDNAphage,ssDNA \\\r\n  -j 2 all\r\n\r\nvirsorter run -w result -i inputs.fna -d db \\\r\n  --min-length 1500 --include-groups dsDNAphage,ssDNA \\\r\n  -j 2 --label original --keep-original-seq classify\r\n<\/code><\/pre>\n
\u8fd9\u6bb5\u5c55\u793a\u5e38\u89c4\u8c03\u7528\u5173\u7cfb\u3002\u590d\u73b0\u5305\u4e2d\u7684\u56fa\u5b9a\u73af\u5883\u91c7\u7528\u5355\u72ec\u5b89\u88c5\u7684\u4f9d\u8d56\uff0c\u5b9e\u9645\u8fd8\u4f7f\u7528\u4e86\u5bf9\u5e94\u7684\u73af\u5883\u8bbe\u7f6e\u4e0e --use-conda-off<\/code>\uff0c\u5b8c\u6574\u547d\u4ee4\u89c1\u5305\u5185\u8bf4\u660e\u3002\u8bfb\u8005\u4e0d\u8981\u53ea\u590d\u5236\u5176\u4e2d\u4e00\u4e2a\u73af\u5883\u9009\u9879\uff0c\u5374\u9057\u6f0f\u5b83\u4f9d\u8d56\u7684\u5b89\u88c5\u6b65\u9aa4\u3002<\/p>\n
\u4e00\u4e2a\u5c24\u5176\u5bb9\u6613\u9057\u6f0f\u7684\u7ec6\u8282\u662f\uff0c\u4fdd\u7559\u539f\u5e8f\u5217\u8fd9\u7ec4\u7684\u8bc4\u5206\u8868 length \u5217\u4ecd\u7136\u8bb0\u5f55\u4fee\u526a\u5019\u9009\u7684\u957f\u5ea6\u3002\u4f8b\u5982\uff0c\u5168\u957f\u8f93\u5165\u5bf9\u5e94\u8bc4\u5206\u8868\u5199\u7684\u662f 47754\uff0c\u4f46\u8be5\u7ec4\u5bfc\u51fa\u7684 FASTA \u5b9e\u9645\u957f 48502\u3002\u56e0\u6b64\uff0c\u51c6\u5907\u4e0b\u6e38\u6587\u4ef6\u65f6\u5e94\u8be5\u5b9e\u9645\u8bfb\u53d6 FASTA \u957f\u5ea6\uff0c\u800c\u4e0d\u662f\u5047\u5b9a\u4efb\u610f\u6a21\u5f0f\u4e0b\u8bc4\u5206\u8868\u7684\u4e00\u5217\u90fd\u4ee3\u8868\u6700\u7ec8\u5bfc\u51fa\u957f\u5ea6\u3002<\/p>\n
\u516d\u3001\u8fb9\u754c\u5750\u6807\u600e\u6837\u6838\u9a8c\uff0c\u624d\u80fd\u907f\u514d\u9519\u4f4d<\/h2>\n
\u9ed8\u8ba4\u8fb9\u754c\u8868\u4e2d\uff0c\u5168\u957f\u8f93\u5165\u7684\u4fee\u526a\u8d77\u6b62\u4e3a 191 \u4e0e 47944\u3002\u6309\u4e00\u8d77\u59cb\u3001\u7ec8\u70b9\u5305\u542b\u7684\u65b9\u5f0f\u8ba1\u7b97\uff0c\u957f\u5ea6\u4e3a 47944 \u51cf 191 \u518d\u52a0\u4e00\uff0c\u5f97\u5230 47754\u3002\u6211\u4eec\u8fdb\u4e00\u6b65\u4ece\u8f93\u5165\u4e2d\u63d0\u53d6\u5bf9\u5e94\u7247\u6bb5\uff0c\u6bd4\u8f83 SHA256\uff0c\u4e0e\u9ed8\u8ba4\u8f93\u51fa\u5b8c\u5168\u4e00\u81f4\u3002<\/p>\n
\u5176\u4f59\u4e09\u6761\u4e5f\u5b8c\u6210\u4e86\u540c\u6837\u6838\u9a8c\u3002\u524d\u534a\u6bb5\u8fb9\u754c\u4e3a 191\u201323918\uff0c\u4e94\u5343\u7247\u6bb5\u4e3a 191\u20134999\uff0c\u4e24\u5343\u7247\u6bb5\u4e3a 191\u20132000\u3002\u4ec5\u9a8c\u8bc1\u957f\u5ea6\u76f8\u7b49\u8fd8\u4e0d\u591f\uff0c\u56e0\u4e3a\u540c\u957f\u5ea6\u7684\u9519\u8bef\u533a\u95f4\u4e5f\u53ef\u80fd\u5b58\u5728\uff0c\u5e8f\u5217\u6821\u9a8c\u503c\u53ef\u4ee5\u68c0\u67e5\u63d0\u53d6\u5185\u5bb9\u662f\u5426\u4e00\u81f4\u3002<\/p>\n
from hashlib import sha256\r\n\r\nstart, end = 191, 47944\r\nfragment = input_sequence[start - 1:end]\r\n\r\nassert len(fragment) == 47754\r\nassert sha256(fragment.encode()).hexdigest() == output_sha256\r\n<\/code><\/pre>\n
\u5c06\u7ed3\u679c\u8f6c\u6210 BED \u6216\u7528\u4e8e\u5176\u4ed6\u5750\u6807\u4f53\u7cfb\u65f6\uff0c\u9700\u8981\u5728\u8fd9\u4e2a\u5df2\u6838\u9a8c\u7684\u57fa\u7840\u4e0a\u8f6c\u6362\u3002\u4e0d\u8981\u628a\u4e00\u79cd\u683c\u5f0f\u7684\u8d77\u70b9\u76f4\u63a5\u590d\u5236\u5230\u53e6\u4e00\u79cd\u683c\u5f0f\uff0c\u518d\u9760\u8089\u773c\u770b\u957f\u5ea6\u5927\u6982\u6b63\u786e\u5c31\u7ed3\u675f\u3002\u5bf9\u4e8e\u4e00\u6761\u91cd\u70b9\u5e8f\u5217\uff0c\u5148\u505a\u9010\u78b1\u57fa\u6216\u6821\u9a8c\u503c\u9a8c\u8bc1\uff0c\u53ef\u4ee5\u5c3d\u65e9\u53d1\u73b0\u7cfb\u7edf\u6027\u504f\u79fb\u3002<\/p>\n
\u8fb9\u754c\u8868\u4e5f\u4e0d\u80fd\u4e00\u6982\u5f53\u4f5c\u6700\u7ec8\u5019\u9009\u6e05\u5355\u3002\u4f5c\u8005\u8bf4\u660e\u5b83\u5305\u542b\u4e2d\u95f4\u4fe1\u606f\uff0c\u67d0\u4e9b\u8bbe\u7f6e\u4e0b\u53ef\u80fd\u51fa\u73b0\u989d\u5916\u8bb0\u5f55\u6216\u4e0e\u6700\u7ec8\u8bc4\u5206\u5b57\u6bb5\u4e0d\u5b8c\u5168\u4e00\u81f4\u7684\u5b57\u6bb5\u3002\u56e0\u6b64\u672c\u6b21\u5148\u4ee5\u6700\u7ec8\u5019\u9009\u540d\u79f0\u548c FASTA \u5bf9\u5e94\uff0c\u518d\u4f7f\u7528\u8fb9\u754c\u8868\u89e3\u91ca\u6765\u6e90\uff0c\u800c\u4e0d\u662f\u628a\u6240\u6709\u8fb9\u754c\u884c\u90fd\u76f4\u63a5\u7b97\u6210\u72ec\u7acb\u75c5\u6bd2\u3002<\/p>\n
\u4e03\u3001\u4e24\u4e2a\u5206\u7c7b\u5668\u90fd\u7ed9\u9ad8\u5206\uff0c\u4e0d\u7b49\u4e8e\u4e24\u79cd\u57fa\u56e0\u7ec4\u7c7b\u578b\u540c\u65f6\u6210\u7acb<\/h2>\n
\u4e24\u5343\u78b1\u57fa\u5019\u9009\u5728 dsDNAphage \u5206\u7c7b\u5668\u4e2d\u7684\u5206\u6570\u4e3a 0.980\uff0c\u5728 ssDNA \u5206\u7c7b\u5668\u4e2d\u4e3a 0.953\u3002\u5df2\u77e5\u5b83\u6765\u81ea\u540c\u4e00\u6761 lambda \u53c2\u8003\uff0c\u4e0d\u80fd\u636e\u6b64\u58f0\u79f0\u6211\u4eec\u627e\u5230\u4e86\u4e00\u4e2a\u540c\u65f6\u5177\u6709\u4e24\u7c7b\u57fa\u56e0\u7ec4\u5c5e\u6027\u7684\u65b0\u5bf9\u8c61\u3002\u8fd9\u6b63\u597d\u8bf4\u660e\u5206\u7c7b\u5668\u5206\u6570\u4e0d\u80fd\u6309\u4e92\u65a5\u5206\u7c7b\u6982\u7387\u76f4\u63a5\u9605\u8bfb\u3002<\/p>\n
\u540c\u6837\uff0c\u6700\u9ad8\u5206\u6240\u5728\u7ec4\u53ea\u662f\u5f53\u524d\u51e0\u4e2a\u5206\u7c7b\u5668\u4e4b\u95f4\u7684\u6bd4\u8f83\u7ed3\u679c\u3002\u82e5\u6539\u53d8\u542f\u7528\u7684\u5206\u7c7b\u5668\u8303\u56f4\uff0c\u53ef\u6bd4\u8f83\u7684\u5bf9\u8c61\u4e5f\u53d8\u4e86\u3002\u505a\u8de8\u6837\u672c\u6c47\u603b\u65f6\u5e94\u8bb0\u5f55\u6240\u7528\u7ec4\u522b\uff0c\u5e76\u5c06\u8fd9\u4e2a\u5b57\u6bb5\u4e0e\u6b63\u5f0f\u5206\u7c7b\u5b66\u6ce8\u91ca\u533a\u522b\u5f00\uff0c\u907f\u514d\u7528\u5b83\u76f4\u63a5\u751f\u6210\u7269\u79cd\u6216\u79d1\u5c5e\u7ec4\u6210\u56fe\u3002<\/p>\n
\u6807\u8bb0\u57fa\u56e0\u6570\u4e5f\u9700\u8981\u7ed3\u5408\u957f\u5ea6\u7406\u89e3\u3002\u5168\u957f\u5019\u9009\u6709\u4e8c\u5341\u4e00\u4e2a\u6807\u8bb0\uff0c\u4e24\u5343\u78b1\u57fa\u5019\u9009\u53ea\u6709\u4e00\u4e2a\uff0c\u4e24\u8005\u90fd\u80fd\u901a\u8fc7\u672c\u6b21\u9ad8\u7f6e\u4fe1\u5ea6\u89c4\u5219\u3002\u4e0d\u80fd\u628a\u6807\u8bb0\u6570\u7b80\u5355\u8f6c\u6362\u6210\u5b8c\u6574\u5ea6\u767e\u5206\u6bd4\uff0c\u56e0\u4e3a\u4e0d\u540c\u5e8f\u5217\u8986\u76d6\u7684\u533a\u57df\u3001\u57fa\u56e0\u7ec4\u6210\u548c\u6240\u7528\u6a21\u578b\u90fd\u4e0d\u540c\u3002<\/p>\n
\u672c\u6b21\u6ca1\u6709\u8bc6\u522b\u5230 partial \u5f62\u5f0f\u7684\u5019\u9009\uff0c\u4e5f\u6ca1\u6709\u6784\u9020\u5bbf\u4e3b\u4e0e\u75c5\u6bd2\u62fc\u63a5\u7684\u8f93\u5165\u3002\u56e0\u6b64\uff0c\u6211\u4eec\u6ca1\u6709\u5b9e\u9645\u6d4b\u51fa\u524d\u75c5\u6bd2\u8fb9\u754c\u8bc6\u522b\u7684\u8868\u73b0\u3002\u82e5\u7814\u7a76\u76ee\u6807\u6b63\u662f\u524d\u75c5\u6bd2\uff0c\u5e94\u53e6\u51c6\u5907\u80fd\u591f\u6838\u5bf9\u8fb9\u754c\u7684\u5bf9\u7167\uff0c\u5e76\u5728\u540e\u7eed\u4f7f\u7528\u4e13\u95e8\u7684\u8d28\u91cf\u8bc4\u4f30\u65b9\u6cd5\u590d\u67e5\u5bbf\u4e3b\u7247\u6bb5\u3002<\/p>\n
\u516b\u3001\u628a\u7ed3\u679c\u4ea4\u7ed9\u4e0b\u6e38\u524d\uff0c\u4fdd\u7559\u54ea\u51e0\u5c42\u4fe1\u606f<\/h2>\n
\u81f3\u5c11\u4fdd\u7559\u539f\u59cb\u7ec4\u88c5\u3001\u9ed8\u8ba4\u5019\u9009 FASTA\u3001\u8bc4\u5206\u8868\u3001\u8fb9\u754c\u8868\u548c\u5b8c\u6574\u547d\u4ee4\u3002\u82e5\u9009\u62e9\u5bfc\u51fa\u539f\u5e8f\u5217\uff0c\u4e5f\u5e94\u4e0e\u4fee\u526a\u7ed3\u679c\u5206\u5f00\u4fdd\u5b58\uff0c\u4e0d\u8981\u8ba9\u540c\u540d\u6587\u4ef6\u5728\u4e0d\u540c\u6b65\u9aa4\u4ee3\u8868\u4e0d\u540c\u78b1\u57fa\u5185\u5bb9\u3002\u5019\u9009\u540d\u79f0\u76f8\u540c\u5e76\u4e0d\u4fdd\u8bc1\u5e8f\u5217\u5185\u5bb9\u76f8\u540c\uff0c\u672c\u6b21\u5df2\u7ecf\u7ed9\u51fa\u4e86\u5b9e\u9645\u4f8b\u5b50\u3002<\/p>\n
\u5bf9\u4e8e\u540e\u7eed\u57fa\u56e0\u6ce8\u91ca\u6216\u8986\u76d6\u8ba1\u7b97\uff0c\u5e94\u660e\u786e\u4f7f\u7528\u54ea\u4e00\u7248\u5e8f\u5217\u3002\u57fa\u4e8e\u4fee\u526a\u5e8f\u5217\u5f97\u5230\u7684\u5750\u6807\uff0c\u4e0d\u80fd\u76f4\u63a5\u5957\u5230\u539f\u59cb\u5e8f\u5217\u4e0a\uff0c\u4e5f\u4e0d\u80fd\u56e0\u4e3a\u540d\u79f0\u76f8\u540c\u5c31\u81ea\u52a8\u62fc\u63a5\u4e0d\u540c\u7ed3\u679c\u3002\u5fc5\u8981\u65f6\u4fdd\u7559\u8f93\u5165\u540d\u79f0\u3001\u5019\u9009\u540d\u79f0\u3001\u8d77\u6b62\u548c\u957f\u5ea6\u7684\u6620\u5c04\u8868\u3002<\/p>\n
VirSorter2 \u7684\u4f18\u52bf\u662f\u80fd\u591f\u63d0\u4f9b\u8f83\u4e30\u5bcc\u7684\u5019\u9009\u8bc1\u636e\u548c\u8fb9\u754c\u4fe1\u606f\uff0c\u5e76\u5141\u8bb8\u590d\u7528\u7279\u5f81\u8c03\u6574\u7b5b\u9009\u3002\u5b83\u7684\u5c40\u9650\u5728\u4e8e\u5019\u9009\u8bc6\u522b\u3001\u5206\u7c7b\u5b66\u8d4b\u503c\u548c\u5b8c\u6574\u5ea6\u8bc4\u4f30\u4ecd\u9700\u5206\u522b\u5b8c\u6210\u3002\u53c2\u6570\u8d8a\u591a\uff0c\u8d8a\u9700\u8981\u56f4\u7ed5\u7814\u7a76\u95ee\u9898\u8bbe\u7f6e\u5bf9\u7167\uff0c\u800c\u4e0d\u662f\u628a\u6240\u6709\u9009\u9879\u540c\u65f6\u6253\u5f00\u540e\u53ea\u770b\u5019\u9009\u6570\u91cf\u3002<\/p>\n
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