{"id":72996,"date":"2026-11-15T13:05:20","date_gmt":"2026-11-15T05:05:20","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=72996"},"modified":"2026-11-15T13:07:31","modified_gmt":"2026-11-15T05:07:31","slug":"salmon-decoy-fragment-count-tpm-composition-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/salmon-decoy-fragment-count-tpm-composition-practice\/","title":{"rendered":"Salmon \u5b9e\u8dd1\u6559\u7a0b\uff5c\u52a0\u5165 decoy \u540e\u6bd4\u5bf9\u7387\u4e0b\u964d\uff0c\u4e3a\u4ec0\u4e48\u5b9a\u91cf\u53cd\u800c\u66f4\u63a5\u8fd1\u8bbe\u8ba1\u771f\u503c"},"content":{"rendered":"
\n

\u8f6c\u5f55\u7ec4\u5b9a\u91cf\u4e2d\uff0c\u4e00\u4e2a\u5bb9\u6613\u8bef\u5bfc\u4eba\u7684\u76ee\u6807\u662f\u5c3d\u91cf\u63d0\u9ad8\u8bfb\u6bb5\u5206\u914d\u6bd4\u4f8b\u3002\u5982\u679c\u53c2\u8003\u91cc\u7f3a\u5c11\u8bfb\u6bb5\u771f\u6b63\u5bf9\u5e94\u7684\u975e\u76ee\u6807\u5e8f\u5217\uff0c\u7a0b\u5e8f\u53ef\u80fd\u628a\u5b83\u5206\u7ed9\u76f8\u8fd1\u8f6c\u5f55\u672c\uff0c\u770b\u8d77\u6765\u5229\u7528\u4e86\u66f4\u591a\u6570\u636e\uff0c\u5374\u540c\u65f6\u62ac\u9ad8\u4e86\u9519\u8bef\u5bf9\u8c61\u7684\u4e30\u5ea6\u3002\u53e6\u4e00\u4e2a\u5e38\u89c1\u8bef\u533a\u662f\u770b\u5230\u67d0\u4e2a\u8f6c\u5f55\u672c\u7684 TPM \u4e0b\u964d\uff0c\u5c31\u76f4\u63a5\u8ba4\u4e3a\u5b83\u81ea\u8eab\u7684\u7247\u6bb5\u6570\u91cf\u4e0b\u964d\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u5b89\u88c5 Salmon 1.10.3\uff0c\u8bbe\u8ba1\u4e09\u4e2a\u4e0d\u540c\u957f\u5ea6\u7684\u8f6c\u5f55\u672c\u548c\u4e00\u4e2a\u4e0e\u5176\u4e2d\u4e4b\u4e00\u76f8\u8fd1\u7684\u975e\u76ee\u6807\u5e8f\u5217\uff0c\u5b8c\u6210\u7d22\u5f15\u548c\u4e09\u7ec4\u5b9a\u91cf\u3002\u5b9e\u9a8c\u540c\u65f6\u5c55\u793a\u4e24\u4ef6\u4e8b\u3002\u52a0\u5165 decoy \u540e\uff0c\u76ee\u6807\u5206\u914d\u6bd4\u4f8b\u4ece\u767e\u5206\u4e4b\u767e\u4e0b\u964d\u5230\u767e\u5206\u4e4b\u516b\u5341\uff0c\u53bb\u6389\u7684\u6070\u597d\u662f\u8bbe\u8ba1\u4e2d\u7684\u975e\u76ee\u6807\u7247\u6bb5\u3002\u53e6\u4e00\u4e2a\u5bf9\u7167\u4e2d\uff0c\u540c\u4e00\u8f6c\u5f55\u672c\u7684\u4f30\u8ba1\u7247\u6bb5\u6570\u4fdd\u6301\u4e00\u5343\uff0cTPM \u5374\u660e\u663e\u4e0b\u964d\u3002<\/p>\n

\"\u5206\u914d\u7387\u4e0b\u964d\uff0c\u5b9a\u91cf\u66f4\u63a5\u8fd1\u8bbe\u8ba1\u771f\u503c\u3002\u52a0\u5165
\u52a0\u5165 decoy\uff0c\u8ffd\u8e2a\u4e94\u767e\u5bf9\u975e\u76ee\u6807\u7247\u6bb5\u7684\u53bb\u5411<\/figcaption><\/figure>\n

\u4e00\u3001\u5148\u628a\u5b9a\u91cf\u5bf9\u8c61\u4e0e\u7ade\u4e89\u53c2\u8003\u5206\u6e05<\/h2>\n

Salmon \u7684\u8f93\u5165\u53c2\u8003\u51b3\u5b9a\u4e86\u7a0b\u5e8f\u53ef\u4ee5\u5c06\u7247\u6bb5\u5206\u914d\u7ed9\u54ea\u4e9b\u76ee\u6807\u3002\u5bf9\u4e8e\u5e38\u89c1\u7684\u8f6c\u5f55\u672c\u5b9a\u91cf\uff0c\u76ee\u6807\u662f\u8f6c\u5f55\u672c\u5e8f\u5217\u3002\u76f8\u4f3c\u5e8f\u5217\u53ef\u80fd\u6765\u81ea\u591a\u4e2a\u8f6c\u5f55\u672c\uff0c\u4e5f\u53ef\u80fd\u6765\u81ea\u76ee\u6807\u96c6\u5408\u4e4b\u5916\u7684\u57fa\u56e0\u7ec4\u533a\u57df\u3002\u540e\u8005\u82e5\u6ca1\u6709\u88ab\u8868\u793a\u5728\u53c2\u8003\u4e2d\uff0c\u5c31\u53ef\u80fd\u9020\u6210\u4e0d\u5408\u7406\u7684\u76ee\u6807\u5206\u914d\u3002<\/p>\n

decoy \u662f\u53c2\u4e0e\u5339\u914d\u7ade\u4e89\u3001\u4f46\u4e0d\u4f5c\u4e3a\u76ee\u6807\u8f6c\u5f55\u672c\u62a5\u544a\u4e30\u5ea6\u7684\u4e00\u7c7b\u53c2\u8003\u3002\u5b83\u5e2e\u52a9\u7a0b\u5e8f\u8bc6\u522b\u67d0\u4e9b\u66f4\u9002\u5408\u7531\u975e\u76ee\u6807\u53c2\u8003\u89e3\u91ca\u7684\u7247\u6bb5\u3002\u4f5c\u8005\u8bf4\u660e\u4ecb\u7ecd\u4e86\u90e8\u5206\u57fa\u56e0\u7ec4 decoy \u548c\u5168\u57fa\u56e0\u7ec4 decoy \u4e24\u79cd\u51c6\u5907\u601d\u8def\uff0c\u5b9e\u9645\u9009\u62e9\u5e94\u4e0e\u7814\u7a76\u5bf9\u8c61\u548c\u53c2\u8003\u5b8c\u6574\u6027\u76f8\u5339\u914d\u3002Salmon \u4f7f\u7528\u8bf4\u660e<\/a><\/p>\n

\u7406\u89e3\u8fd9\u4e2a\u673a\u5236\u540e\uff0c\u5c31\u4e0d\u5e94\u628a decoy \u770b\u4f5c\u201c\u964d\u4f4e\u6570\u636e\u5229\u7528\u7387\u7684\u5f00\u5173\u201d\u3002\u5b83\u6539\u53d8\u4e86\u54ea\u4e9b\u7247\u6bb5\u88ab\u8ba4\u4e3a\u9002\u5408\u652f\u6301\u76ee\u6807\u5b9a\u91cf\u3002\u67d0\u6b21\u5206\u914d\u6bd4\u4f8b\u4e0b\u964d\u662f\u5426\u5408\u7406\uff0c\u4ecd\u9700\u8981\u770b\u8f93\u5165\u3001\u7ade\u4e89\u5e8f\u5217\u548c\u8fc7\u6ee4\u539f\u56e0\uff0c\u4e0d\u80fd\u5355\u51ed\u4e0b\u964d\u6216\u4e0a\u5347\u5224\u65ad\u8d28\u91cf\u3002<\/p>\n

\u672c\u6b21\u91c7\u7528\u56fa\u5b9a\u7248\u672c\u548c\u955c\u50cf\u6458\u8981\uff0c\u6240\u6709\u7ed3\u679c\u90fd\u6765\u81ea\u540c\u4e00\u5957\u8f6f\u4ef6\u3002\u5728\u7ebf\u6587\u6863\u53ef\u80fd\u968f\u7740\u7248\u672c\u66f4\u65b0\uff0c\u590d\u73b0\u65f6\u5e94\u4ee5\u5305\u5185\u8bb0\u5f55\u7684\u5b9e\u9645\u547d\u4ee4\u548c\u8f93\u51fa\u5b57\u6bb5\u4e3a\u51c6\u3002\u5c24\u5176\u4e0d\u8981\u628a\u4e00\u4e2a\u65b0\u7248\u672c\u7684\u5168\u90e8\u884c\u4e3a\u81ea\u52a8\u5f52\u5230\u672c\u6587\u4f7f\u7528\u7684\u7248\u672c\u4e0a\u3002<\/p>\n

\u4e8c\u3001\u4eba\u5de5\u6570\u636e\u600e\u6837\u8bbe\u8ba1\uff0c\u4e3a\u4ec0\u4e48\u8981\u8fd9\u6837\u8bbe\u8ba1<\/h2>\n

\u4e09\u4e2a\u76ee\u6807\u8f6c\u5f55\u672c\u5206\u522b\u957f\u4e09\u5343\u3001\u4e00\u5343\u4e94\u767e\u548c\u516d\u5343\u78b1\u57fa\uff0c\u8bb0\u4f5c txA\u3001txB\u3001txC\u3002\u5176\u5e8f\u5217\u6309\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u751f\u6210\uff0c\u5f7c\u6b64\u4e0d\u523b\u610f\u5171\u4eab\u7247\u6bb5\u3002\u6211\u4eec\u518d\u590d\u5236 txA\uff0c\u5e76\u6cbf\u5e8f\u5217\u6bcf\u9694\u516b\u5341\u78b1\u57fa\u66ff\u6362\u4e00\u4e2a\u4f4d\u7f6e\uff0c\u5171\u6539\u53d8\u4e09\u5341\u4e03\u4e2a\u78b1\u57fa\uff0c\u5f97\u5230 decoyA\u3002<\/p>\n

\u8fd9\u4e2a\u975e\u76ee\u6807\u5e8f\u5217\u4e0e txA \u5f88\u76f8\u8fd1\uff0c\u4f46\u5e76\u4e0d\u5b8c\u5168\u76f8\u540c\u3002\u8f93\u5165\u7247\u6bb5\u5982\u679c\u6765\u81ea decoyA\uff0c\u5728\u53ea\u542b\u76ee\u6807\u7684\u53c2\u8003\u4e2d\u4ecd\u53ef\u80fd\u88ab txA \u63a5\u7eb3\uff0c\u800c\u52a0\u5165\u51c6\u786e\u7684 decoy \u540e\u5c31\u6709\u4e86\u66f4\u5408\u9002\u7684\u7ade\u4e89\u5bf9\u8c61\u3002\u8fd9\u4e2a\u8bbe\u8ba1\u7528\u6765\u5c55\u793a\u53c2\u8003\u7f3a\u5931\u7684\u5f71\u54cd\uff0c\u4e0d\u4ee3\u8868\u771f\u5b9e\u6837\u672c\u4e2d\u7684\u67d0\u79cd\u5177\u4f53\u6c61\u67d3\u6216\u57fa\u56e0\u5bb6\u65cf\u3002<\/p>\n

\u57fa\u7ebf\u6587\u5e93\u7531\u4e24\u5343\u4e94\u767e\u5bf9\u65e0\u6d4b\u5e8f\u9519\u8bef\u7684\u8bfb\u6bb5\u7ec4\u6210\uff0c\u6bcf\u7aef\u4e00\u767e\u78b1\u57fa\uff0c\u7247\u6bb5\u957f\u5ea6\u5728\u4e8c\u767e\u4e8c\u5341\u81f3\u4e8c\u767e\u516b\u5341\u4e4b\u95f4\u3002\u751f\u6210\u65f6\u5148\u62bd\u53d6\u5b8c\u6574\u7247\u6bb5\uff0c\u518d\u53d6\u6b63\u5411\u9996\u7aef\u548c\u53cd\u5411\u4e92\u8865\u672b\u7aef\uff0c\u6700\u540e\u5c06\u7247\u6bb5\u987a\u5e8f\u6253\u4e71\u3002\u4e24\u4e2a FASTQ \u6587\u4ef6\u59cb\u7ec8\u6210\u5bf9\u4fdd\u5b58\uff0c\u4e0d\u80fd\u5404\u81ea\u72ec\u7acb\u6253\u4e71\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n\n
\u7247\u6bb5\u6765\u6e90<\/th>\n\u57fa\u7ebf\u6587\u5e93<\/th>\n\u589e\u52a0 B \u7684\u6587\u5e93<\/th>\n<\/tr>\n<\/thead>\n
txA<\/td>\n1000 \u5bf9<\/td>\n1000 \u5bf9<\/td>\n<\/tr>\n
txB<\/td>\n500 \u5bf9<\/td>\n1000 \u5bf9<\/td>\n<\/tr>\n
txC<\/td>\n500 \u5bf9<\/td>\n500 \u5bf9<\/td>\n<\/tr>\n
decoyA<\/td>\n500 \u5bf9<\/td>\n500 \u5bf9<\/td>\n<\/tr>\n
\u603b\u8ba1<\/td>\n2500 \u5bf9<\/td>\n3000 \u5bf9<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u7b2c\u4e8c\u4e2a\u6587\u5e93\u4fdd\u6301 A\u3001C \u548c\u975e\u76ee\u6807\u7247\u6bb5\u6570\u91cf\u4e0d\u53d8\uff0c\u53ea\u589e\u52a0 B \u7684\u6570\u91cf\uff0c\u7528\u6765\u89c2\u5bdf\u7ec4\u6210\u53d8\u5316\u3002\u4e24\u4e2a\u6587\u5e93\u5404\u81ea\u91cd\u65b0\u62bd\u53d6\u7247\u6bb5\u4f4d\u7f6e\uff0c\u56e0\u6b64\u5b83\u4eec\u4e0d\u662f\u9010\u6761\u8bfb\u6bb5\u5b8c\u5168\u76f8\u540c\u7684\u590d\u5236\u3002\u6211\u4eec\u56fa\u5b9a\u7684\u662f\u6765\u6e90\u6570\u91cf\u4e0e\u751f\u6210\u89c4\u5219\uff0c\u800c\u4e0d\u662f\u5047\u88c5\u6240\u6709\u5176\u4ed6\u89c2\u6d4b\u7ec6\u8282\u90fd\u5b8c\u5168\u4e0d\u53d8\u3002<\/p>\n

\u4e09\u3001\u7d22\u5f15\u6587\u4ef6\u600e\u6837\u7ec4\u7ec7\uff0c\u907f\u514d decoy \u6ca1\u6709\u771f\u6b63\u751f\u6548<\/h2>\n

\u5b9e\u9a8c\u5efa\u7acb\u4e86\u4e24\u4e2a\u7d22\u5f15\u3002\u7b2c\u4e00\u4e2a\u53ea\u5305\u542b\u4e09\u4e2a\u76ee\u6807\u8f6c\u5f55\u672c\uff0c\u7b2c\u4e8c\u4e2a\u5728\u76ee\u6807\u540e\u8ffd\u52a0 decoyA\uff0c\u5e76\u901a\u8fc7\u72ec\u7acb\u540d\u5355\u58f0\u660e\u5b83\u7684\u540d\u79f0\u3002\u4ec5\u628a\u5e8f\u5217\u8ffd\u52a0\u5230 FASTA \u800c\u4e0d\u6b63\u786e\u58f0\u660e\uff0c\u5c31\u53ef\u80fd\u628a\u5b83\u5f53\u4f5c\u666e\u901a\u76ee\u6807\uff0c\u6539\u53d8\u5b9a\u91cf\u5bf9\u8c61\u7684\u542b\u4e49\u3002<\/p>\n

salmon index -t transcripts.fa -i index_plain -k 31 -p 2\r\n\r\nsalmon index -t gentrome.fa -i index_decoy \\\r\n  --decoys decoys.txt -k 31 -p 2\r\n\r\nsalmon quant -i index_decoy -l ISF \\\r\n  -1 baseline_1.fq -2 baseline_2.fq \\\r\n  -p 2 --validateMappings --dumpEq \\\r\n  -o with_decoy\r\n<\/code><\/pre>\n
\u8fd9\u7ec4\u8bfb\u6bb5\u7684\u65b9\u5411\u662f\u6211\u4eec\u4e3b\u52a8\u8bbe\u8ba1\u7684\uff0c\u56e0\u6b64\u4f7f\u7528\u4e0e\u8bbe\u8ba1\u4e00\u81f4\u7684 ISF\u3002\u672c\u6587\u6ca1\u6709\u4f9d\u8d56\u81ea\u52a8\u63a8\u65ad\u6587\u5e93\u7c7b\u578b\uff0c\u4e5f\u6ca1\u6709\u5bf9\u4e0d\u540c\u65b9\u5411\u8bbe\u7f6e\u505a\u6027\u80fd\u6bd4\u8f83\u3002\u771f\u5b9e\u6587\u5e93\u5e94\u6839\u636e\u5b9e\u9a8c\u65b9\u6848\u548c\u8f93\u51fa\u68c0\u67e5\u786e\u5b9a\u65b9\u5411\uff0c\u4e0d\u80fd\u56e0\u4e3a\u67d0\u4e2a\u793a\u4f8b\u7528\u4e86\u8fd9\u4e2a\u503c\u5c31\u76f4\u63a5\u590d\u5236\u3002<\/p>\n
\u8f93\u5165\u53c2\u8003\u540d\u79f0\u4e5f\u5fc5\u987b\u4ed4\u7ec6\u68c0\u67e5\u3002\u540d\u5355\u4e2d\u7684\u540d\u79f0\u8981\u80fd\u7cbe\u786e\u5bf9\u5e94 FASTA \u4e2d\u7684\u5e8f\u5217\u6807\u8bc6\uff0c\u76ee\u6807\u8f6c\u5f55\u672c\u4e0e decoy \u4e0d\u5e94\u56e0\u4e3a\u547d\u540d\u5904\u7406\u4ea7\u751f\u6df7\u6dc6\u3002\u5bf9\u4e8e\u6765\u81ea\u4e0d\u540c\u6570\u636e\u5e93\u7684\u53c2\u8003\uff0c\u6807\u9898\u88c1\u526a\u3001\u7248\u672c\u540e\u7f00\u548c\u91cd\u590d\u6807\u8bc6\u90fd\u503c\u5f97\u5728\u5efa\u7acb\u7d22\u5f15\u524d\u6838\u5bf9\u3002<\/p>\n
\u4e24\u5957\u7d22\u5f15\u5171\u7528\u76f8\u540c\u7684\u76ee\u6807\u5e8f\u5217\uff0c\u56e0\u6b64\u57fa\u7ebf\u6587\u5e93\u7684\u524d\u4e24\u7ec4\u5dee\u5f02\u4e3b\u8981\u5bf9\u5e94 decoy \u662f\u5426\u53c2\u4e0e\u7ade\u4e89\u3002\u82e5\u540c\u65f6\u6362\u4e86\u76ee\u6807\u8f6c\u5f55\u672c\u7248\u672c\uff0c\u5c31\u65e0\u6cd5\u5c06\u7ed3\u679c\u53d8\u5316\u7b80\u5355\u5f52\u56e0\u4e8e decoy\u3002\u53ef\u89e3\u91ca\u7684\u5bf9\u7167\u9700\u8981\u5c3d\u91cf\u51cf\u5c11\u540c\u65f6\u6539\u53d8\u7684\u6761\u4ef6\u3002<\/p>\n
\u672c\u6b21\u8fd8\u6838\u5bf9\u4e86\u6587\u5e93\u683c\u5f0f\u8bca\u65ad\u6587\u4ef6\uff0c\u4e09\u7ec4\u5df2\u7ecf\u5206\u914d\u7684\u7247\u6bb5\u90fd\u4e0e\u6240\u6307\u5b9a\u65b9\u5411\u517c\u5bb9\uff0c\u6ca1\u6709\u51fa\u73b0\u4e0d\u4e00\u81f4\u65b9\u5411\u6216\u5b64\u7acb\u7aef\u6620\u5c04\u8ba1\u6570\u3002\u8fd9\u9879\u68c0\u67e5\u5e2e\u52a9\u786e\u8ba4\u672c\u6b21\u5bf9\u7167\u6ca1\u6709\u540c\u65f6\u6df7\u5165\u660e\u663e\u7684\u914d\u5bf9\u65b9\u5411\u95ee\u9898\uff0c\u4f46\u5b83\u4e0d\u6784\u6210\u5bf9\u6240\u6709\u771f\u5b9e\u6587\u5e93\u8d28\u91cf\u7684\u4fdd\u8bc1\u3002<\/p>\n
\u5728\u81ea\u5df1\u7684\u9879\u76ee\u4e2d\uff0c\u53ef\u5c06\u8fd9\u7c7b\u8bca\u65ad\u4e0e\u6837\u672c\u6e05\u5355\u5173\u8054\u4fdd\u5b58\u3002\u6bcf\u4e2a\u6837\u672c\u8bb0\u5f55\u6587\u5e93\u7c7b\u578b\u3001\u4e24\u4e2a\u8f93\u5165\u6587\u4ef6\u3001\u76ee\u6807\u7d22\u5f15\u548c\u5b9e\u9645\u547d\u4ee4\uff0c\u53d1\u73b0\u5f02\u5e38\u65f6\u5148\u5b9a\u4f4d\u5230\u5177\u4f53\u6837\u672c\u3002\u628a\u4e0d\u540c\u6837\u672c\u7684\u4e24\u4e2a\u672b\u7aef\u8bef\u914d\u6210\u4e00\u7ec4\uff0c\u4e5f\u53ef\u80fd\u4ea7\u51fa\u6587\u4ef6\uff0c\u5355\u7eaf\u68c0\u67e5\u6587\u4ef6\u662f\u5426\u5b58\u5728\u65e0\u6cd5\u53d1\u73b0\u8fd9\u79cd\u8f93\u5165\u9519\u8bef\u3002<\/p>\n
\u56db\u3001\u5b9e\u9645\u5206\u914d\u7ed3\u679c\uff0c\u5c11\u4e86\u4e94\u767e\u5bf9\u610f\u5473\u7740\u4ec0\u4e48<\/h2>\n
\u53ea\u542b\u76ee\u6807\u7684\u7d22\u5f15\u5c06\u57fa\u7ebf\u6587\u5e93\u7684\u4e24\u5343\u4e94\u767e\u5bf9\u7247\u6bb5\u5168\u90e8\u5206\u914d\u7ed9\u4e86\u76ee\u6807\uff0c\u5176\u4e2d A \u5f97\u5230\u4e00\u5343\u4e94\u767e\uff0cB \u548c C \u5404\u4e94\u767e\u3002\u52a0\u5165 decoy \u540e\uff0cA \u56de\u5230\u4e00\u5343\uff0cB\u3001C \u4ecd\u5404\u4e94\u767e\uff0c\u53e6\u6709\u4e94\u767e\u5bf9\u88ab\u8bb0\u4e3a decoy \u7247\u6bb5\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u6761\u4ef6<\/th>\nA \u7247\u6bb5<\/th>\nB \u7247\u6bb5<\/th>\nC \u7247\u6bb5<\/th>\ndecoy<\/th>\n<\/tr>\n<\/thead>\n
\u57fa\u7ebf\uff0c\u4e0d\u542b decoy<\/td>\n1500<\/td>\n500<\/td>\n500<\/td>\n0<\/td>\n<\/tr>\n
\u57fa\u7ebf\uff0c\u542b decoy<\/td>\n1000<\/td>\n500<\/td>\n500<\/td>\n500<\/td>\n<\/tr>\n
\u589e\u52a0 B\uff0c\u542b decoy<\/td>\n1000<\/td>\n1000<\/td>\n500<\/td>\n500<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8fd9\u91cc\u53ef\u4ee5\u660e\u786e\u5224\u65ad\u4e94\u767e\u5bf9\u7684\u53bb\u5411\uff0c\u662f\u56e0\u4e3a\u8bbe\u8ba1\u65f6\u4fdd\u5b58\u4e86\u6765\u6e90\u771f\u503c\u3002\u76ee\u6807\u5206\u914d\u6bd4\u4f8b\u4e0b\u964d\u5e76\u4e0d\u610f\u5473\u7740\u968f\u673a\u4e22\u5931\u4e86\u4e94\u767e\u5bf9\u76ee\u6807\u7247\u6bb5\uff0c\u800c\u662f\u6b63\u786e\u8bc6\u522b\u4e86\u672c\u6b21\u7279\u610f\u52a0\u5165\u7684\u975e\u76ee\u6807\u6765\u6e90\u3002\u8fd9\u662f\u4e00\u4e2a\u53d7\u63a7\u5b9e\u9a8c\u7684\u7ed3\u8bba\uff0c\u4e0d\u80fd\u63a8\u5e7f\u6210\u201c\u4efb\u4f55\u5206\u914d\u7387\u4e0b\u964d\u90fd\u4ee3\u8868\u7ed3\u679c\u66f4\u597d\u201d\u3002<\/p>\n
\u5b9e\u9645\u9879\u76ee\u4e2d\uff0c\u5e94\u540c\u65f6\u67e5\u770b\u5904\u7406\u7247\u6bb5\u603b\u6570\u3001\u5206\u914d\u5230\u76ee\u6807\u7684\u7247\u6bb5\u6570\u3001\u88ab decoy \u5438\u6536\u7684\u7247\u6bb5\u6570\u53ca\u5176\u4ed6\u8fc7\u6ee4\u4fe1\u606f\u3002\u82e5\u6bd4\u4f8b\u7a81\u7136\u6539\u53d8\uff0c\u53ef\u4ee5\u5148\u68c0\u67e5\u53c2\u8003\u662f\u5426\u53d8\u5316\u3001\u540d\u5355\u662f\u5426\u6b63\u786e\u4ee5\u53ca\u8f93\u5165\u662f\u5426\u6765\u81ea\u76f8\u540c\u6587\u5e93\u3002\u5355\u72ec\u622a\u53d6\u4e00\u4e2a\u767e\u5206\u6bd4\u7528\u4e8e\u6bd4\u8f83\uff0c\u4f1a\u63a9\u76d6\u8fd9\u4e9b\u4e0d\u540c\u539f\u56e0\u3002<\/p>\n
\u8fd8\u8981\u533a\u5206\u8bfb\u6bb5\u7aef\u6570\u4e0e\u7247\u6bb5\u6570\u3002\u57fa\u7ebf\u6709\u4e24\u5343\u4e94\u767e\u5bf9\uff0c\u4e5f\u5c31\u662f\u4e94\u5343\u6761\u5355\u7aef\u8bfb\u6bb5\u3002\u5728\u8fd9\u4e2a\u53cc\u7aef\u5b9a\u91cf\u5b9e\u9a8c\u4e2d\uff0c\u7ed3\u679c\u4e2d\u7684\u6570\u91cf\u5bf9\u5e94\u914d\u5bf9\u7247\u6bb5\u7684\u4f30\u8ba1\u5206\u914d\uff0c\u4e0d\u80fd\u518d\u56e0\u4e3a\u6709\u4e24\u4e2a FASTQ \u5c31\u628a\u6bcf\u4e2a\u76ee\u6807\u7684\u6570\u91cf\u4e58\u4e8c\u3002<\/p>\n
\u4e94\u3001\u4e3a\u4ec0\u4e48\u4e24\u4e2a\u76ee\u6807\u7247\u6bb5\u6570\u76f8\u540c\uff0cTPM \u5dee\u5f88\u591a<\/h2>\n
\u52a0\u5165 decoy \u540e\uff0cB \u4e0e C \u90fd\u5f97\u5230\u4e94\u767e\u4e2a\u4f30\u8ba1\u7247\u6bb5\uff0c\u4f46 TPM \u5206\u522b\u7ea6\u4e3a\u56db\u5341\u4e03\u4e07\u548c\u5341\u4e07\u3002\u539f\u56e0\u53ef\u4ee5\u4ece\u5b83\u4eec\u7684\u6709\u6548\u957f\u5ea6\u770b\u51fa\u6765\u3002\u672c\u6b21 B \u7684\u6709\u6548\u957f\u5ea6\u7ea6\u4e3a 1249.937\uff0cC \u7ea6\u4e3a 5749.937\uff0c\u76f8\u540c\u7247\u6bb5\u6570\u5e76\u4e0d\u610f\u5473\u7740\u76f8\u540c\u7684\u957f\u5ea6\u6821\u6b63\u4e30\u5ea6\u3002<\/p>\n
\u6709\u6548\u957f\u5ea6\u4e0e\u53c2\u8003\u5e8f\u5217\u7684\u5b9e\u9645\u957f\u5ea6\u76f8\u5173\uff0c\u4f46\u4e0d\u662f\u7b80\u5355\u590d\u5236\u90a3\u4e00\u5217\u3002\u5b83\u8fd8\u53cd\u6620\u5f53\u524d\u6a21\u578b\u5bf9\u53ef\u4ea7\u751f\u89c2\u6d4b\u7247\u6bb5\u673a\u4f1a\u7684\u5904\u7406\u3002\u9605\u8bfb\u8f93\u51fa\u65f6\u5e94\u540c\u65f6\u4fdd\u7559 Length\u3001EffectiveLength\u3001NumReads \u548c TPM\uff0c\u4e0d\u80fd\u5c06\u5b83\u4eec\u5f53\u4f5c\u53ef\u4ee5\u76f8\u4e92\u66ff\u6362\u7684\u4e0d\u540c\u540d\u5b57\u3002\u8f93\u51fa\u683c\u5f0f\u8bf4\u660e<\/a><\/p>\n
\u5728\u672c\u6b21\u672a\u542f\u7528\u989d\u5916\u504f\u501a\u6821\u6b63\u7684\u7b80\u5316\u5b9e\u9a8c\u4e2d\uff0c\u53ef\u4ee5\u7528\u4f30\u8ba1\u7247\u6bb5\u6570\u9664\u4ee5\u6709\u6548\u957f\u5ea6\uff0c\u518d\u5c06\u6240\u6709\u76ee\u6807\u7684\u7ed3\u679c\u5f52\u4e00\u5230\u4e00\u767e\u4e07\uff0c\u590d\u67e5\u8f93\u51fa TPM\u3002\u8fd9\u6837\u505a\u7684\u4ef7\u503c\u5728\u4e8e\u7406\u89e3\u5206\u6bcd\uff0c\u800c\u4e0d\u662f\u9f13\u52b1\u5728\u4efb\u4f55\u590d\u6742\u6a21\u578b\u4e0b\u90fd\u624b\u5de5\u66ff\u4ee3\u8f6f\u4ef6\u7684\u8ba1\u7b97\u3002<\/p>\n
import csv\r\n\r\nwith open("with_decoy\/quant.sf") as handle:\r\n    rows = list(csv.DictReader(handle, delimiter="\\t"))\r\n\r\nrates = [float(r["NumReads"]) \/ float(r["EffectiveLength"]) for r in rows]\r\ndenominator = sum(rates)\r\n\r\nfor row, rate in zip(rows, rates):\r\n    print(row["Name"], rate \/ denominator * 1_000_000, row["TPM"])\r\n<\/code><\/pre>\n
\u7531\u4e8e\u8f93\u51fa\u4e2d\u7684\u6709\u6548\u957f\u5ea6\u5df2\u7ecf\u7ecf\u8fc7\u683c\u5f0f\u5316\uff0c\u624b\u7b97\u672b\u5c3e\u53ef\u80fd\u51fa\u73b0\u5f88\u5c0f\u7684\u5dee\u5f02\uff0c\u5e94\u4f7f\u7528\u5408\u7406\u8bef\u5dee\u8303\u56f4\u6bd4\u8f83\u3002\u82e5\u5dee\u5f02\u5f88\u5927\uff0c\u4f18\u5148\u68c0\u67e5\u8bfb\u9519\u5217\u3001\u5b57\u7b26\u4e32\u8f6c\u6570\u5b57\u3001\u9057\u6f0f\u76ee\u6807\u6216\u5c06\u5b9e\u9645\u957f\u5ea6\u8bef\u5f53\u6709\u6548\u957f\u5ea6\u7b49\u95ee\u9898\uff0c\u4e0d\u5fc5\u7acb\u523b\u6000\u7591\u6a21\u578b\u5931\u6548\u3002<\/p>\n
\u516d\u3001A \u7684\u7247\u6bb5\u6570\u6ca1\u6709\u53d8\uff0cTPM \u4e3a\u4ec0\u4e48\u4e0b\u964d<\/h2>\n
\u5728\u542b decoy \u7684\u57fa\u7ebf\u7ed3\u679c\u4e2d\uff0cA \u7684 TPM \u4e3a 427504.313640\u3002\u589e\u52a0 B \u7684\u6587\u5e93\u4e2d\uff0cA \u4ecd\u5f97\u5230\u4e00\u5343\u4e2a\u4f30\u8ba1\u7247\u6bb5\uff0c\u4f46 TPM \u964d\u5230 290748.918588\u3002C \u540c\u6837\u4fdd\u6301\u4e94\u767e\u4e2a\u7247\u6bb5\uff0cTPM \u4e5f\u4ece 102228.076478 \u964d\u5230 69523.119148\u3002<\/p>\n
\u8fd9\u5e76\u4e0d\u77db\u76fe\u3002TPM \u8868\u793a\u5f53\u524d\u76ee\u6807\u96c6\u5408\u5185\u7684\u76f8\u5bf9\u4e30\u5ea6\uff0c\u5176\u4ed6\u5bf9\u8c61\u589e\u52a0\u4f1a\u6539\u53d8\u5f52\u4e00\u5316\u5206\u6bcd\u3002A \u81ea\u5df1\u7684\u7247\u6bb5\u6570\u4fdd\u6301\u4e0d\u53d8\uff0c\u4ecd\u7136\u53ef\u80fd\u5360\u636e\u66f4\u5c0f\u7684\u6bd4\u4f8b\u3002\u6bcf\u4e2a\u7ed3\u679c\u6587\u4ef6\u7684 TPM \u603b\u548c\u90fd\u63a5\u8fd1\u4e00\u767e\u4e07\uff0c\u5e76\u4e0d\u80fd\u8bc1\u660e\u6bcf\u4e2a\u76ee\u6807\u90fd\u5177\u6709\u76f8\u540c\u7684\u7edd\u5bf9\u8868\u8fbe\u80cc\u666f\u3002<\/p>\n
\n\n\n\n\n\n\n
\u542b decoy \u7684\u6587\u5e93<\/th>\nA \u7684\u7247\u6bb5\u6570<\/th>\nA \u7684 TPM<\/th>\nB \u7684\u7247\u6bb5\u6570<\/th>\n<\/tr>\n<\/thead>\n
\u57fa\u7ebf<\/td>\n1000<\/td>\n427504.313640<\/td>\n500<\/td>\n<\/tr>\n
\u589e\u52a0 B<\/td>\n1000<\/td>\n290748.918588<\/td>\n1000<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u5728\u771f\u5b9e\u751f\u7269\u5b66\u6570\u636e\u4e2d\uff0c\u6211\u4eec\u901a\u5e38\u6ca1\u6709\u8fd9\u79cd\u9010\u6765\u6e90\u7684\u8bbe\u8ba1\u771f\u503c\uff0c\u56e0\u6b64\u66f4\u9700\u8981\u8c28\u614e\u89e3\u91ca\u3002\u4ec5\u51ed TPM \u4e0b\u964d\uff0c\u4e0d\u80fd\u5355\u72ec\u63a8\u51fa\u7ec6\u80de\u5185\u8be5\u8f6c\u5f55\u672c\u7684\u7edd\u5bf9\u5206\u5b50\u6570\u4e0b\u964d\u3002\u5b9e\u9a8c\u8bbe\u8ba1\u3001\u53d6\u6837\u91cf\u3001\u6574\u4f53 RNA \u7ec4\u6210\u548c\u6240\u91c7\u7528\u7684\u7edf\u8ba1\u5206\u6790\u4f1a\u5171\u540c\u5f71\u54cd\u80fd\u591f\u56de\u7b54\u7684\u95ee\u9898\u3002<\/p>\n
\u540c\u6837\u4e0d\u80fd\u7528\u672c\u6b21\u4eba\u4e3a\u4fdd\u6301\u7247\u6bb5\u6570\u4e0d\u53d8\u7684\u8bbe\u8ba1\uff0c\u53bb\u63a8\u65ad\u6240\u6709\u771f\u5b9e\u6837\u672c\u90fd\u5b58\u5728\u76f8\u540c\u53d8\u5316\u673a\u5236\u3002\u8fd9\u4e2a\u5bf9\u7167\u5c55\u793a\u7684\u662f\u4e00\u4e2a\u53ef\u80fd\u53d1\u751f\u4e14\u80fd\u591f\u8ba1\u7b97\u6e05\u695a\u7684\u7ec4\u6210\u6548\u5e94\uff0c\u63d0\u9192\u8bfb\u8005\u68c0\u67e5\u81ea\u5df1\u7684\u7ed3\u8bba\u662f\u5426\u8d85\u51fa\u4e86\u76f8\u5bf9\u91cf\u6240\u652f\u6301\u7684\u8303\u56f4\u3002<\/p>\n
\u4e03\u3001\u4ece\u8f6c\u5f55\u672c\u7ed3\u679c\u8fdb\u5165\u4e0b\u6e38\u5206\u6790\u65f6\uff0c\u5148\u6838\u5bf9\u6807\u8bc6\u4e0e\u5355\u4f4d<\/h2>\n
Salmon \u7684\u76ee\u6807\u6807\u8bc6\u6765\u81ea\u53c2\u8003\u8f6c\u5f55\u672c\u3002\u5982\u679c\u540e\u7eed\u9700\u8981\u6309\u57fa\u56e0\u6c47\u603b\uff0c\u5c31\u8981\u51c6\u5907\u4e0e\u8fd9\u5957\u53c2\u8003\u76f8\u5bf9\u5e94\u7684\u8f6c\u5f55\u672c\u5230\u57fa\u56e0\u5173\u7cfb\u3002\u968f\u610f\u5220\u9664\u7248\u672c\u540e\u7f00\u3001\u6df7\u7528\u53e6\u4e00\u4e2a\u6ce8\u91ca\u7248\u672c\u6216\u91cd\u590d\u5339\u914d\u540c\u4e00\u8f6c\u5f55\u672c\uff0c\u90fd\u53ef\u80fd\u5236\u9020\u9519\u8bef\u5408\u5e76\u6216\u6f0f\u9879\u3002<\/p>\n
\u5bf9\u4e8e\u57fa\u56e0\u6c34\u5e73\u5dee\u5f02\u5206\u6790\uff0c\u53ef\u9605\u8bfb tximport \u7684\u6b63\u5f0f\u5bfc\u5165\u6d41\u7a0b\uff0c\u660e\u786e\u600e\u6837\u540c\u65f6\u5904\u7406\u4f30\u8ba1\u8ba1\u6570\u3001\u4e30\u5ea6\u548c\u957f\u5ea6\u4fe1\u606f\u3002tximport \u6559\u7a0b<\/a> \u4e0d\u5e94\u770b\u5230\u8f93\u51fa\u6709\u5c0f\u6570\u5c31\u968f\u610f\u56db\u820d\u4e94\u5165\uff0c\u66f4\u4e0d\u5e94\u628a TPM \u5217\u6539\u540d\u4e3a\u539f\u59cb\u8ba1\u6570\u540e\u76f4\u63a5\u9001\u5165\u8981\u6c42\u8ba1\u6570\u8f93\u5165\u7684\u6d41\u7a0b\u3002<\/p>\n
\u672c\u6b21\u6ca1\u6709\u6267\u884c\u5dee\u5f02\u8868\u8fbe\u68c0\u9a8c\uff0c\u6ca1\u6709\u751f\u7269\u5b66\u91cd\u590d\uff0c\u4e5f\u6ca1\u6709\u7ed9\u51fa\u663e\u8457\u6027\u7ed3\u8bba\u3002\u4e09\u4e2a\u6761\u4ef6\u662f\u673a\u5236\u5bf9\u7167\uff0c\u4e0d\u80fd\u5305\u88c5\u6210\u5904\u7406\u7ec4\u4e0e\u5bf9\u7167\u7ec4\u7684\u771f\u5b9e\u5b9e\u9a8c\u3002\u5b83\u4eec\u7684\u4f5c\u7528\u662f\u5e2e\u52a9\u8bfb\u8005\u7406\u89e3\u8f93\u5165\u53c2\u8003\u548c\u5f52\u4e00\u5316\u5982\u4f55\u6539\u53d8\u5b9a\u91cf\u7ed3\u679c\u3002<\/p>\n
\u5982\u679c\u53ea\u662f\u5236\u4f5c\u63a2\u7d22\u56fe\uff0c\u53ef\u4ee5\u4f7f\u7528\u6e05\u6670\u6ce8\u660e\u5355\u4f4d\u7684\u76f8\u5bf9\u4e30\u5ea6\u3002\u4f46\u56fe\u6ce8\u4ecd\u5e94\u4ea4\u4ee3\u53c2\u8003\u3001\u5b9a\u91cf\u5de5\u5177\u7248\u672c\u548c\u6570\u636e\u6765\u6e90\u3002\u4e00\u4e2a\u6f02\u4eae\u7684\u70ed\u56fe\u4e0d\u4f1a\u81ea\u52a8\u8bf4\u660e\u76ee\u6807\u662f\u5426\u76f8\u540c\u3001\u6837\u672c\u662f\u5426\u53ef\u6bd4\uff0c\u4ee5\u53ca\u989c\u8272\u53d8\u5316\u662f\u5426\u5bf9\u5e94\u7edd\u5bf9\u6570\u91cf\u53d8\u5316\u3002<\/p>\n
\u516b\u3001\u600e\u6837\u8bc4\u4ef7 Salmon\uff0c\u5e76\u628a\u5c0f\u5b9e\u9a8c\u7ecf\u9a8c\u7528\u4e8e\u6b63\u5f0f\u9879\u76ee<\/h2>\n
Salmon \u7684\u4f18\u52bf\u662f\u80fd\u76f4\u63a5\u4ece\u8bfb\u6bb5\u5f97\u5230\u8f6c\u5f55\u672c\u4e30\u5ea6\u53ca\u76f8\u5173\u8bca\u65ad\u4fe1\u606f\uff0c\u9002\u5408\u5728\u56fa\u5b9a\u53c2\u8003\u4e0b\u6279\u91cf\u5904\u7406\u6837\u672c\u3002\u5b83\u4e5f\u63d0\u4f9b\u7ade\u4e89\u53c2\u8003\u548c\u591a\u79cd\u6a21\u578b\u9009\u9879\uff0c\u8ba9\u5b9a\u91cf\u4e0d\u53ea\u4f9d\u8d56\u6700\u7b80\u5355\u7684\u5e8f\u5217\u5339\u914d\u3002\u5bf9\u4e8e\u8bfb\u8005\u6765\u8bf4\uff0c\u771f\u6b63\u7684\u6536\u76ca\u662f\u80fd\u591f\u4fdd\u7559\u8f93\u5165\u4e0e\u8f93\u51fa\u4e4b\u95f4\u7684\u89e3\u91ca\u94fe\u6761\u3002<\/p>\n
\u5b83\u7684\u5c40\u9650\u540c\u6837\u6e05\u695a\u3002\u76ee\u6807\u4e0d\u5728\u53c2\u8003\u4e2d\uff0c\u5c31\u65e0\u6cd5\u6309\u6b63\u786e\u76ee\u6807\u62a5\u544a\u3002\u76f8\u4f3c\u8f6c\u5f55\u672c\u7684\u4fe1\u606f\u4e0d\u8db3\uff0c\u4e0d\u4f1a\u56e0\u4e3a\u8f93\u51fa\u4fdd\u7559\u5f88\u591a\u5c0f\u6570\u800c\u6d88\u5931\u3002decoy \u4e0d\u5b8c\u6574\u6216\u4e0d\u5408\u9002\uff0c\u4e5f\u53ef\u80fd\u7559\u4e0b\u95ee\u9898\u3002\u672c\u6b21\u6ca1\u6709\u52a0\u5165\u590d\u6742\u540c\u6e90\u5bb6\u65cf\u3001\u6d4b\u5e8f\u9519\u8bef\u6216\u771f\u5b9e\u504f\u501a\uff0c\u56e0\u6b64\u7ed3\u679c\u4e0d\u80fd\u4f5c\u4e3a\u6240\u6709\u573a\u666f\u7684\u51c6\u786e\u7387\u8bc1\u660e\u3002<\/p>\n
\u5b9e\u9645\u5de5\u4f5c\u4e2d\u53ef\u5148\u62bd\u53d6\u5c11\u91cf\u6837\u672c\uff0c\u6bd4\u8f83\u5408\u7406\u7684\u53c2\u8003\u65b9\u6848\uff0c\u5e76\u9010\u9879\u68c0\u67e5\u76ee\u6807\u6570\u3001\u7247\u6bb5\u53bb\u5411\u3001\u6709\u6548\u957f\u5ea6\u548c\u5173\u952e\u8f6c\u5f55\u672c\u53d8\u5316\u3002\u786e\u8ba4\u89e3\u91ca\u4e00\u81f4\u540e\u518d\u6269\u5c55\u5230\u5b8c\u6574\u961f\u5217\u3002\u6240\u6709\u6837\u672c\u6700\u597d\u4f7f\u7528\u540c\u4e00\u5957\u53c2\u8003\u548c\u53c2\u6570\uff0c\u786e\u9700\u53d8\u66f4\u65f6\u4fdd\u7559\u7248\u672c\u5e76\u91cd\u65b0\u8bc4\u4f30\u53ef\u6bd4\u6027\u3002<\/p>\n
\u4e0b\u8f7d\u672c\u7ec4\u590d\u73b0\u6750\u6599<\/a>\u53ef\u83b7\u5f97\u56fa\u5b9a\u955c\u50cf\u3001\u4e09\u4e2a\u76ee\u6807\u548c decoy \u7684\u751f\u6210\u89c4\u5219\u3001\u7247\u6bb5\u6765\u6e90\u6570\u91cf\u3001\u4e24\u5957\u7d22\u5f15\u547d\u4ee4\u4e0e\u4e09\u7ec4\u5b8c\u6574\u7ed3\u679c\uff0c\u8bfb\u8005\u53ef\u4ee5\u91cd\u65b0\u6838\u5bf9\u6587\u4e2d\u7684\u6bcf\u4e2a\u6570\u5b57\u3002<\/p>\n
\u5f53\u8f6c\u5f55\u672c\u5b9a\u91cf\u9700\u8981\u8986\u76d6\u66f4\u591a\u6837\u672c\uff0c\u5e76\u6301\u7eed\u56fa\u5b9a\u53c2\u8003\u3001\u8bb0\u5f55\u53c2\u6570\u548c\u4fdd\u5b58\u7ed3\u679c\u7248\u672c\u65f6\uff0c\u53ef\u4ee5\u4e86\u89e3\u4e91\u751f\u4fe1\u751f\u7269\u4fe1\u606f\u5206\u6790\u73af\u5883\u670d\u52a1<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u901a\u8fc7\u4e09\u7ec4\u5b9e\u9645\u5b9a\u91cf\u89e3\u91ca decoy \u7684\u4f5c\u7528\u3001\u53cc\u7aef\u7247\u6bb5\u8ba1\u6570\u3001\u6709\u6548\u957f\u5ea6\u548c TPM \u7ec4\u6210\u6548\u5e94\uff0c\u9644\u8f93\u5165\u751f\u6210\u4e0e\u7ed3\u679c\u6838\u9a8c\u811a\u672c\u3002<\/p>\n","protected":false},"author":1,"featured_media":72995,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[80,552,1341,1299],"class_list":["post-72996","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-rna-seq","tag-salmon","tag-tpm","tag-1299"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/salmon-decoy-fragment-count-tpm-composition-practice-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72996","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=72996"}],"version-history":[{"count":2,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72996\/revisions"}],"predecessor-version":[{"id":73006,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/72996\/revisions\/73006"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/72995"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=72996"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=72996"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=72996"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}