{"id":73033,"date":"2026-11-15T13:25:47","date_gmt":"2026-11-15T05:25:47","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73033"},"modified":"2026-11-15T13:26:55","modified_gmt":"2026-11-15T05:26:55","slug":"rsem-shared-reads-gene-grouping-isopct-credible-interval-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/rsem-shared-reads-gene-grouping-isopct-credible-interval-practice\/","title":{"rendered":"RSEM \u5b9e\u8dd1\uff0c\u4ece\u5171\u4eab\u8bfb\u6bb5\u5230\u57fa\u56e0\u6c47\u603b\uff0c\u8bfb\u61c2\u671f\u671b\u8ba1\u6570\u548c\u53ef\u4fe1\u533a\u95f4"},"content":{"rendered":"
\n

\u4e00\u6761\u8bfb\u6bb5\u540c\u65f6\u5339\u914d\u4e24\u4e2a\u8f6c\u5f55\u672c\uff0c\u80fd\u5426\u7ed9\u4e24\u4e2a\u8f6c\u5f55\u672c\u5404\u52a0\u4e00\uff1f\u5982\u679c\u8fd9\u6837\u505a\uff0c\u540c\u4e00\u4efd\u6d4b\u5e8f\u8bc1\u636e\u5c31\u88ab\u91cd\u590d\u8ba1\u5165\u3002\u5168\u90e8\u4e22\u5f03\u53c8\u4f1a\u635f\u5931\u5171\u4eab\u533a\u57df\u7684\u4fe1\u606f\u3002RSEM \u5904\u7406\u7684\u6838\u5fc3\u95ee\u9898\uff0c\u662f\u5728\u660e\u786e\u6a21\u578b\u5047\u8bbe\u540e\uff0c\u628a\u4e0d\u80fd\u552f\u4e00\u5b9a\u4f4d\u7684\u8bc1\u636e\u7eb3\u5165\u8868\u8fbe\u4f30\u8ba1\uff0c\u5e76\u8f93\u51fa\u57fa\u56e0\u548c\u8f6c\u5f55\u672c\u4e24\u4e2a\u5c42\u7ea7\u7684\u7ed3\u679c\u3002<\/p>\n

\u672c\u7bc7\u5b9e\u9645\u51c6\u5907\u4e09\u6761\u4eba\u5de5\u8f6c\u5f55\u672c\u3001\u751f\u6210\u4e00\u5343\u5bf9\u8bfb\u6bb5\u3001\u5b8c\u6210\u8f6c\u5f55\u672c\u5750\u6807\u6bd4\u5bf9\uff0c\u518d\u8fd0\u884c\u4e24\u7ec4 RSEM \u5b9a\u91cf\u3002\u4e24\u7ec4\u53ea\u6539\u53d8\u8f6c\u5f55\u672c\u4e0e\u57fa\u56e0\u7684\u5bf9\u5e94\u5173\u7cfb\uff0c\u89c2\u5bdf\u671f\u671b\u8ba1\u6570\u3001\u57fa\u56e0\u5185\u6bd4\u4f8b\u548c\u53ef\u4fe1\u533a\u95f4\u3002\u8fd0\u884c\u4e2d\u8fd8\u9047\u5230\u4e00\u4e2a\u771f\u5b9e\u7684\u6784\u5efa\u95ee\u9898\uff0c\u6211\u4eec\u4fdd\u7559\u8bca\u65ad\u8bb0\u5f55\u5e76\u4fee\u590d\u540e\u91cd\u65b0\u8dd1\u901a\uff0c\u6ca1\u6709\u628a\u5f02\u5e38\u4e2d\u95f4\u6587\u4ef6\u5f53\u6210\u6709\u6548\u7ed3\u679c\u3002<\/p>\n

\"\u6bd4\u4f8b\u53d8\u4e86\uff0c\u8bfb\u6bb5\u53ef\u80fd\u6ca1\u6709\u53d8\u3002\u540c\u4e00\u5343\u5bf9\u8bfb\u6bb5\uff0c\u4e24\u79cd\u57fa\u56e0\u5206\u7ec4\u4e0e\u53ef\u4fe1\u533a\u95f4\u5bf9\u7167\u3002330
\u540c\u4e00\u5343\u5bf9\u8bfb\u6bb5\uff0c\u4e24\u79cd\u57fa\u56e0\u5206\u7ec4\u4e0e\u53ef\u4fe1\u533a\u95f4\u5bf9\u7167<\/figcaption><\/figure>\n

\u5148\u660e\u786e\u8f93\u51fa\u6570\u5b57\u7684\u5355\u4f4d<\/h2>\n

RSEM \u7684\u671f\u671b\u8ba1\u6570\u53ef\u4ee5\u5305\u542b\u5c0f\u6570\uff0c\u56e0\u4e3a\u5b83\u8868\u793a\u6839\u636e\u6a21\u578b\u5206\u914d\u5230\u67d0\u4e2a\u76ee\u6807\u7684\u8bfb\u6bb5\u6216\u7247\u6bb5\u8d21\u732e\u3002\u672c\u7bc7\u4f7f\u7528\u53cc\u7aef\u6570\u636e\uff0c\u6240\u4ee5\u8ba8\u8bba\u7684\u662f\u7247\u6bb5\u3002\u4e00\u4e2a\u5171\u4eab\u7247\u6bb5\u53ef\u4ee5\u6309\u6743\u91cd\u5f71\u54cd\u591a\u4e2a\u5019\u9009\uff0c\u4f46\u5b83\u4e0d\u662f\u5728\u6bcf\u4e2a\u5019\u9009\u4e2d\u90fd\u88ab\u5b8c\u6574\u590d\u5236\u4e00\u6b21\u3002<\/p>\n

\u8f6c\u5f55\u672c\u4e30\u5ea6\u3001\u57fa\u56e0\u4e30\u5ea6\u548c\u57fa\u56e0\u5185\u6bd4\u4f8b\u4e5f\u4e0d\u662f\u540c\u4e00\u4e2a\u5bf9\u8c61\u3002\u524d\u4e24\u8005\u5206\u522b\u5bf9\u5e94\u5355\u6761\u8f6c\u5f55\u672c\u4e0e\u540c\u4e00\u57fa\u56e0\u4e0b\u7684\u4e00\u7ec4\u8f6c\u5f55\u672c\uff0c\u57fa\u56e0\u5185\u6bd4\u4f8b\u5219\u9700\u8981\u660e\u786e\u5206\u6bcd\u662f\u54ea\u4e00\u7ec4\u6210\u5458\u3002\u82e5\u5206\u7ec4\u6587\u4ef6\u9519\u8bef\uff0c\u5373\u4f7f\u5e8f\u5217\u6bd4\u5bf9\u548c\u8f6c\u5f55\u672c\u8ba1\u6570\u770b\u8d77\u6765\u6b63\u5e38\uff0c\u57fa\u56e0\u5c42\u7ea7\u89e3\u91ca\u4ecd\u7136\u53ef\u80fd\u5931\u771f\u3002<\/p>\n

\u8fd9\u4e00\u70b9\u6bd4\u80cc\u8bb0\u8f93\u51fa\u5217\u540d\u66f4\u91cd\u8981\u3002\u8bfb\u8005\u5728\u6574\u7406\u7ed3\u679c\u65f6\uff0c\u5e94\u5148\u5199\u51fa\u6bcf\u4e00\u5217\u7684\u5bf9\u8c61\u548c\u5355\u4f4d\uff0c\u518d\u51b3\u5b9a\u600e\u6837\u6bd4\u8f83\u6837\u672c\u6216\u8005\u6c47\u603b\u57fa\u56e0\u3002\u65b9\u6cd5\u80cc\u666f\u53ef\u53c2\u9605 RSEM \u539f\u59cb\u8bba\u6587<\/a>\uff0c\u5176\u4e2d\u591a\u91cd\u5339\u914d\u8bc1\u636e\u7684\u5904\u7406\u662f\u7406\u89e3\u5de5\u5177\u5b9a\u4f4d\u7684\u5173\u952e\u3002<\/p>\n

\u4e00\u5343\u4e2a\u7247\u6bb5\u91cc\uff0c\u56db\u767e\u4e2a\u6ca1\u6709\u552f\u4e00\u6765\u6e90<\/h2>\n

\u4eba\u5de5\u8f6c\u5f55\u672c A \u548c B \u90fd\u957f\u4e24\u5343\u78b1\u57fa\uff0c\u524d\u4e00\u5343\u4e03\u767e\u78b1\u57fa\u5b8c\u5168\u76f8\u540c\uff0c\u672b\u7aef\u4e09\u767e\u78b1\u57fa\u4e0d\u540c\u3002\u7b2c\u4e09\u6761\u8f6c\u5f55\u672c C \u72ec\u7acb\u751f\u6210\uff0c\u4e5f\u957f\u4e24\u5343\u78b1\u57fa\u3002\u53cc\u7aef\u8bfb\u957f\u4e3a\u4e00\u767e\uff0c\u7247\u6bb5\u957f\u5ea6\u5728\u4e8c\u767e\u4e8c\u5341\u5230\u4e8c\u767e\u516b\u5341\u4e4b\u95f4\uff0c\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u751f\u6210\u5e76\u6253\u4e71\u987a\u5e8f\u3002<\/p>\n

\u6211\u4eec\u4ece\u5171\u4eab\u533a\u57df\u751f\u6210\u56db\u767e\u4e2a\u7247\u6bb5\uff0c\u4ece A \u7684\u7279\u6709\u533a\u57df\u751f\u6210\u4e00\u767e\u4e00\u5341\u4e2a\uff0c\u4ece B \u7684\u7279\u6709\u533a\u57df\u751f\u6210\u4e5d\u5341\u4e2a\uff0c\u518d\u4ece C \u751f\u6210\u56db\u767e\u4e2a\u3002\u56e0\u4e3a A \u548c B \u7684\u5171\u4eab\u90e8\u5206\u76f8\u540c\uff0c\u90a3\u56db\u767e\u4e2a\u7247\u6bb5\u4e0d\u80fd\u4ec5\u9760\u5e8f\u5217\u88ab\u552f\u4e00\u5206\u5230\u5176\u4e2d\u4e00\u65b9\u3002\u5b83\u4eec\u9700\u8981\u4e0e\u7279\u6709\u533a\u57df\u7684\u8bc1\u636e\u4e00\u8d77\u89e3\u91ca\u3002<\/p>\n

\u8fd9\u91cc\u6545\u610f\u6309\u533a\u57df\u9009\u62e9\u7247\u6bb5\uff0c\u8986\u76d6\u5e76\u975e\u5747\u5300\u7684\u771f\u5b9e\u8f6c\u5f55\u7ec4\u62bd\u6837\uff0c\u4e5f\u6ca1\u6709\u52a0\u5165\u6d4b\u5e8f\u9519\u8bef\u3002\u56e0\u6b64\uff0c\u672c\u6b21\u8bbe\u8ba1\u9002\u5408\u8bf4\u660e\u5206\u914d\u548c\u6c47\u603b\uff0c\u4e0d\u9002\u5408\u62ff\u4f30\u8ba1\u7ed3\u679c\u8bc4\u5224\u5de5\u5177\u7684\u771f\u5b9e\u8868\u8fbe\u51c6\u786e\u7387\u3002\u7279\u522b\u662f\u4e0d\u80fd\u628a\u4eba\u5de5\u6307\u5b9a\u7684\u7279\u6709\u7247\u6bb5\u6bd4\u4f8b\u76f4\u63a5\u5f53\u6210\u6240\u6709\u5b9e\u9a8c\u90fd\u6ee1\u8db3\u7684\u8868\u8fbe\u771f\u503c\u3002<\/p>\n

\u540c\u4e00\u6279\u8f6c\u5f55\u672c\uff0c\u51c6\u5907\u4e24\u79cd\u57fa\u56e0\u5173\u7cfb<\/h2>\n

\u7b2c\u4e00\u7ec4\u628a A \u548c B \u653e\u5728\u57fa\u56e0 AB \u4e0b\uff0cC \u5355\u72ec\u5c5e\u4e8e\u57fa\u56e0 C\u3002\u7b2c\u4e8c\u7ec4\u4e0d\u63d0\u4f9b\u5206\u7ec4\u8868\uff0c\u4e5f\u4e0d\u63d0\u4f9b\u57fa\u56e0\u7ec4\u6ce8\u91ca\uff0c\u76f4\u63a5\u4f7f\u7528\u8f6c\u5f55\u672c\u5e8f\u5217\u5efa\u7acb\u53c2\u8003\u3002\u6309\u8fd9\u4e00\u8def\u5f84\u7684\u9ed8\u8ba4\u89c4\u5219\uff0c\u6bcf\u6761\u5e8f\u5217\u4f1a\u88ab\u770b\u4f5c\u5355\u72ec\u7684\u57fa\u56e0\u3002<\/p>\n

RSEM \u7684\u5bf9\u5e94\u6587\u4ef6\u5148\u5199\u57fa\u56e0\u7f16\u53f7\uff0c\u518d\u5199\u8f6c\u5f55\u672c\u7f16\u53f7\uff0c\u4e2d\u95f4\u7528\u5236\u8868\u7b26\u5206\u9694\u3002\u6587\u4ef6\u540d\u867d\u7136\u542b\u6709\u8f6c\u5f55\u672c\u5230\u57fa\u56e0\u7684\u542b\u4e49\uff0c\u5217\u987a\u5e8f\u5374\u4e0d\u80fd\u51ed\u540d\u79f0\u731c\u3002\u590d\u7528\u5176\u4ed6\u5206\u6790\u5de5\u5177\u7684\u5bf9\u5e94\u8868\u4e4b\u524d\uff0c\u5fc5\u987b\u6838\u5bf9\u65b9\u5411\u548c\u662f\u5426\u5305\u542b\u8868\u5934\u3002\u5b98\u65b9\u53c2\u8003\u51c6\u5907\u8bf4\u660e<\/a><\/p>\n

printf 'geneAB\\ttxA\\ngeneAB\\ttxB\\ngeneC\\ttxC\\n' > gene_map.tsv\r\n<\/code><\/pre>\n
rsem-prepare-reference \\\r\n  --transcript-to-gene-map gene_map.tsv \\\r\n  transcripts.fa grouped\/ref\r\n\r\nrsem-prepare-reference transcripts.fa separate\/ref\r\n<\/code><\/pre>\n
\u4e24\u7ec4\u751f\u6210\u7684\u8f6c\u5f55\u672c\u5e8f\u5217\u7d22\u5f15\u6458\u8981\u5b8c\u5168\u76f8\u540c\uff0c\u5dee\u522b\u5728\u5206\u7ec4\u5173\u7cfb\u3002\u56e0\u6b64\uff0c\u540e\u9762\u770b\u5230\u6bd4\u4f8b\u53d8\u5316\u65f6\uff0c\u53ef\u4ee5\u6392\u9664\u53c2\u8003\u5e8f\u5217\u672c\u8eab\u88ab\u4fee\u6539\u8fd9\u4e00\u89e3\u91ca\u3002\u771f\u5b9e\u9879\u76ee\u4e5f\u5e94\u91c7\u7528\u7c7b\u4f3c\u601d\u8def\uff0c\u5206\u522b\u4fdd\u5b58\u5e8f\u5217\u7248\u672c\u548c\u6ce8\u91ca\u5bf9\u5e94\u5173\u7cfb\uff0c\u4e0d\u80fd\u53ea\u8bb0\u5f55\u4e00\u4e2a\u542b\u7cca\u7684\u53c2\u8003\u540d\u79f0\u3002<\/p>\n
\u8f93\u5165\u8981\u6c42\u6bd4\u201c\u5df2\u7ecf\u6709 BAM\u201d\u66f4\u5177\u4f53<\/h2>\n
RSEM \u63a5\u6536\u5df2\u6709\u6bd4\u5bf9\u65f6\uff0c\u5bf9\u8bb0\u5f55\u7ec4\u7ec7\u6709\u660e\u786e\u8981\u6c42\u3002\u540c\u4e00\u8bfb\u6bb5\u7684\u591a\u4e2a\u6bd4\u5bf9\u5e94\u653e\u5728\u4e00\u8d77\uff0c\u53cc\u7aef\u6bcf\u4e2a\u6bd4\u5bf9\u4f4d\u7f6e\u7684\u4e24\u4e2a mate \u8fd8\u5e94\u76f8\u90bb\uff0c\u5e8f\u5217\u548c\u8d28\u91cf\u5b57\u6bb5\u4e0d\u80fd\u7f3a\u5931\u3002\u5e38\u89c1\u7684\u57fa\u56e0\u7ec4\u5750\u6807\u6392\u5e8f BAM \u5e76\u4e0d\u81ea\u52a8\u6ee1\u8db3\u8fd9\u4e9b\u8981\u6c42\u3002<\/p>\n
\u672c\u7bc7\u4f7f\u7528 HISAT2 \u5bf9\u8f6c\u5f55\u672c\u5e8f\u5217\u8fdb\u884c\u975e\u526a\u63a5\u6bd4\u5bf9\uff0c\u7981\u7528\u8f6f\u526a\u5207\u3001\u6df7\u5408\u914d\u5bf9\u548c\u4e0d\u4e00\u81f4\u914d\u5bf9\uff0c\u5e76\u7528\u5f88\u9ad8\u7684\u7f3a\u53e3\u60e9\u7f5a\u907f\u514d\u63d2\u5165\u5220\u9664\u3002\u5728\u8fd9\u4efd\u65e0\u9519\u8bef\u7684\u8bbe\u8ba1\u6570\u636e\u4e2d\uff0c\u5b9e\u9645 CIGAR \u5168\u90e8\u662f\u5b8c\u6574\u7684\u4e00\u767e\u78b1\u57fa\u5339\u914d\u3002\u4e24\u7ec4\u8f93\u5165\u90fd\u901a\u8fc7 RSEM \u81ea\u5e26\u9a8c\u8bc1\u5668\u3002<\/p>\n
hisat2-build -p 2 grouped\/ref.idx.fa grouped\/hisat\r\nhisat2 -p 2 --no-spliced-alignment --no-softclip \\\r\n  --no-mixed --no-discordant \\\r\n  --rdg 999999,999999 --rfg 999999,999999 \\\r\n  -k 20 --seed 144 -x grouped\/hisat \\\r\n  -1 reads1.fq -2 reads2.fq -S grouped\/alignments.sam\r\nrsem-sam-validator grouped\/alignments.sam\r\n<\/code><\/pre>\n
\u6700\u7ec8 SAM \u6709\u4e8c\u5343\u516b\u767e\u6761\u8bb0\u5f55\uff0c\u4f46\u53ea\u6709\u4e00\u5343\u4e2a\u7247\u6bb5\u540d\u79f0\u3002\u516d\u767e\u4e2a\u5177\u6709\u552f\u4e00\u5339\u914d\u7684\u7247\u6bb5\u5404\u4ea7\u751f\u4e24\u6761\u8bb0\u5f55\uff0c\u56db\u767e\u4e2a\u5171\u4eab\u7247\u6bb5\u5404\u4ea7\u751f\u4e24\u4e2a\u4f4d\u7f6e\u3001\u6bcf\u4e2a\u4f4d\u7f6e\u4e24\u6761\u8bb0\u5f55\u3002\u8bb0\u5f55\u6570\u589e\u52a0\u5e76\u4e0d\u610f\u5473\u7740\u6d4b\u5e8f\u91cf\u589e\u52a0\u3002\u8fd9\u6b21\u6838\u5bf9\u540c\u65f6\u786e\u8ba4\u4e86\u5019\u9009\u6bd4\u5bf9\u6ca1\u6709\u88ab\u8bef\u5f53\u6210\u989d\u5916\u5206\u5b50\u3002<\/p>\n
\u8fd9\u4e9b\u53c2\u6570\u7528\u4e8e\u672c\u6b21\u8f93\u5165\u7ea6\u675f\u6f14\u793a\uff0c\u5e76\u4e0d\u662f\u5efa\u8bae\u5bf9\u6240\u6709 RNA \u6570\u636e\u5173\u95ed\u526a\u63a5\u3002\u8fd9\u91cc\u6bd4\u5bf9\u7684\u662f\u5df2\u7ecf\u526a\u63a5\u597d\u7684\u8f6c\u5f55\u672c\u5e8f\u5217\uff0c\u82e5\u628a\u76f8\u540c\u547d\u4ee4\u76f4\u63a5\u7528\u5728\u771f\u5b9e\u57fa\u56e0\u7ec4\u4e0a\uff0c\u8f93\u5165\u5bf9\u8c61\u5c31\u53d8\u4e86\u3002\u6b63\u5f0f\u6d41\u7a0b\u5e94\u9009\u62e9\u4e0e\u53c2\u8003\u6784\u5efa\u65b9\u5f0f\u5339\u914d\u7684\u6bd4\u5bf9\u8def\u5f84\uff0c\u518d\u6838\u5bf9 RSEM \u5b9a\u91cf\u8f93\u5165\u8bf4\u660e<\/a>\u3002<\/p>\n
\u5b89\u88c5\u65f6\u9047\u5230\u7684\u5d29\u6e83\u600e\u6837\u5904\u7406<\/h2>\n
\u672c\u6b21\u57fa\u7840\u5bb9\u5668\u7684\u8f6f\u4ef6\u5305\u5143\u6570\u636e\u4e3a\u4e00\u70b9\u4e09\u70b9\u4e09\uff0c\u6784\u5efa\u53f7\u4ee5\u5341\u4e8c\u7ed3\u5c3e\uff0c\u4f46\u547d\u4ee4\u884c\u7248\u672c\u6a2a\u5e45\u81ea\u62a5\u4e00\u70b9\u4e09\u70b9\u4e00\u3002\u6211\u4eec\u540c\u65f6\u4fdd\u5b58\u4e86\u8f6f\u4ef6\u5305\u4fe1\u606f\u3001\u955c\u50cf\u6458\u8981\u548c\u5b9e\u9645\u6a2a\u5e45\uff0c\u6ca1\u6709\u4efb\u9009\u5176\u4e2d\u4e00\u4e2a\u5f53\u6210\u5b8c\u6574\u7684\u73af\u5883\u8bb0\u5f55\u3002<\/p>\n
\u66f4\u91cd\u8981\u7684\u662f\uff0c\u539f\u59cb\u955c\u50cf\u5728\u8ba1\u7b97\u53ef\u4fe1\u533a\u95f4\u65f6\u9000\u51fa\u7801\u4e3a\u4e00\u767e\u4e09\u5341\u4e5d\uff0c\u4e2d\u95f4\u62bd\u6837\u6587\u4ef6\u51fa\u73b0\u975e\u6709\u9650\u6570\u503c\u3002\u6362\u7528\u53e6\u4e00\u4e2a\u8f83\u65e9\u6784\u5efa\u4e5f\u6ca1\u6709\u89e3\u51b3\u3002\u6b64\u65f6\u5373\u4f7f\u8f93\u51fa\u76ee\u5f55\u5df2\u7ecf\u6709\u8868\u8fbe\u8868\uff0c\u4e5f\u4e0d\u80fd\u628a\u6574\u4e2a\u5206\u6790\u89c6\u4e3a\u6210\u529f\uff0c\u5c24\u5176\u4e0d\u80fd\u5f15\u7528\u5176\u4e2d\u5f02\u5e38\u7684\u540e\u9a8c\u4e30\u5ea6\u3002<\/p>\n
\u6211\u4eec\u68c0\u67e5\u56fa\u5b9a\u7248\u672c\u5b98\u65b9\u6e90\u7801\uff0c\u53d1\u73b0\u6709\u4e9b\u8bfb\u6587\u4ef6\u64cd\u4f5c\u4f4d\u4e8e\u65ad\u8a00\u8868\u8fbe\u5f0f\u5185\u90e8\u3002\u5bf9\u540c\u4e00\u4efd\u6e90\u7801\u505a\u63a7\u5236\u7f16\u8bd1\uff0c\u663e\u5f0f\u5173\u95ed\u65ad\u8a00\u80fd\u591f\u590d\u73b0\u5d29\u6e83\uff0c\u4fdd\u7559\u65ad\u8a00\u5219\u6b63\u5e38\u5b8c\u6210\u3002\u539f\u955c\u50cf\u4e2d\u7684\u76f8\u5173\u7a0b\u5e8f\u4e5f\u672a\u68c0\u51fa\u65ad\u8a00\u8c03\u7528\u7b26\u53f7\u3002\u8fd9\u4e9b\u8bc1\u636e\u652f\u6301\u65ad\u8a00\u79fb\u9664\u5bfc\u81f4\u8fd0\u884c\u5f02\u5e38\u7684\u5224\u65ad\uff0c\u4f46\u5e76\u975e\u5bf9\u6240\u6709\u53d1\u5e03\u7248\u672c\u7684\u6982\u62ec\uff0c\u4e5f\u4e0d\u7b49\u4e8e\u62ff\u5230\u4e86\u539f\u59cb\u6784\u5efa\u65e5\u5fd7\u3002<\/p>\n
\u6700\u7ec8\u4ece\u5b98\u65b9\u4e00\u70b9\u4e09\u70b9\u4e09\u6e90\u7801\u91cd\u65b0\u7f16\u8bd1 Gibbs \u62bd\u6837\u548c\u53ef\u4fe1\u533a\u95f4\u4e24\u4e2a\u7a0b\u5e8f\uff0c\u660e\u786e\u4fdd\u7559\u65ad\u8a00\uff0c\u4f7f\u7528\u53ea\u8bfb\u6302\u8f7d\u66ff\u6362\u672c\u6b21\u8fd0\u884c\u7684\u5bf9\u5e94\u7a0b\u5e8f\u3002\u6e90\u7801\u6ca1\u6709\u4fee\u6539\uff0c\u57fa\u7840\u955c\u50cf\u4e5f\u6ca1\u6709\u88ab\u8986\u76d6\u3002\u968f\u540e\u4ece\u5934\u91cd\u8dd1\u4e24\u7ec4\u5b8c\u6574\u5b9a\u91cf\uff0c\u5747\u6210\u529f\u9000\u51fa\u5e76\u5f97\u5230\u6709\u9650\u7684\u540e\u9a8c\u4f30\u8ba1\u548c\u533a\u95f4\u3002\u672c\u6b21\u6838\u5bf9\u7684\u56fa\u5b9a\u7248\u672c\u6e90\u7801<\/a><\/p>\n
\u516c\u5f00\u811a\u672c\u5305\u542b\u6e90\u7801\u4e0b\u8f7d\u6458\u8981\u68c0\u67e5\u3001\u8fd9\u4e24\u4e2a\u7a0b\u5e8f\u7684\u7f16\u8bd1\u3001\u8f93\u5165\u751f\u6210\u548c\u5168\u90e8\u8fd0\u884c\u6b65\u9aa4\uff0c\u9700\u8981 Linux\u3001Docker\u3001Python\uff0c\u4ee5\u53ca\u5177\u5907\u9759\u6001\u94fe\u63a5\u5e93\u7684 C++ \u7f16\u8bd1\u73af\u5883\u3002\u5b83\u4f1a\u5728\u8f93\u51fa\u76ee\u5f55\u4e2d\u6784\u5efa\u6240\u9700\u6587\u4ef6\u3002\u5355\u72ec\u62c9\u53d6\u4e0b\u9762\u7684\u539f\u59cb\u955c\u50cf\u5e76\u4e0d\u80fd\u590d\u73b0\u672c\u6587\u4fee\u590d\u540e\u7684\u53ef\u4fe1\u533a\u95f4\u7ed3\u679c\uff0c\u5b8c\u6574\u590d\u73b0\u5e94\u6267\u884c\u9644\u4ef6\u4e2d\u7684\u811a\u672c\u3002<\/p>\n
docker pull quay.io\/biocontainers\/rsem:1.3.3--pl5321h077b44d_12\r\n\r\n# \u89e3\u538b\u590d\u73b0\u5305\u540e\uff0c\u5728\u5bf9\u5e94\u5de5\u5177\u76ee\u5f55\u6267\u884c\r\n# \u811a\u672c\u4f1a\u6309\u56fa\u5b9a\u6e90\u7801\u91cd\u5efa Gibbs \u548c\u53ef\u4fe1\u533a\u95f4\u7a0b\u5e8f\r\npython3 run.py .\/practice\r\n<\/code><\/pre>\n
\u5171\u4eab\u7247\u6bb5\u88ab\u600e\u6837\u5206\u914d<\/h2>\n
\u4fee\u590d\u73af\u5883\u540e\uff0c\u5206\u7ec4\u53c2\u8003\u4e0b A \u7684\u671f\u671b\u8ba1\u6570\u4e3a\u4e09\u767e\u4e8c\u5341\u4e5d\u70b9\u4e5d\u4e5d\uff0cB \u4e3a\u4e8c\u767e\u4e03\u5341\u70b9\u96f6\u4e00\uff0cC \u4e3a\u56db\u767e\u3002A \u4e0e B \u7684\u548c\u4e3a\u516d\u767e\uff0c\u57fa\u56e0 AB \u7684\u8f93\u51fa\u4e5f\u4e3a\u516d\u767e\u3002\u8fd9\u8bf4\u660e\u56db\u767e\u4e2a\u5171\u4eab\u7247\u6bb5\u88ab\u7eb3\u5165\u4e86\u5206\u914d\uff0c\u6ca1\u6709\u5411 A \u548c B \u5404\u81ea\u91cd\u590d\u589e\u52a0\u56db\u767e\u4efd\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
\u76ee\u6807<\/th>\n\u671f\u671b\u8ba1\u6570<\/th>\n\u57fa\u56e0\u5185\u6bd4\u4f8b<\/th>\n<\/tr>\n<\/thead>\n
A<\/td>\n329.99<\/td>\n55%<\/td>\n<\/tr>\n
B<\/td>\n270.01<\/td>\n45%<\/td>\n<\/tr>\n
C<\/td>\n400.00<\/td>\n100%<\/td>\n<\/tr>\n
\u57fa\u56e0 AB<\/td>\n600.00<\/td>\n\u4e0d\u9002\u7528<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8fd9\u7ec4\u7b80\u5355\u8bbe\u8ba1\u4e2d\uff0c\u4e24\u6761\u76f8\u540c\u957f\u5ea6\u8f6c\u5f55\u672c\u7684\u7279\u6709\u8bc1\u636e\u5206\u522b\u4e3a\u4e00\u767e\u4e00\u5341\u548c\u4e5d\u5341\uff0c\u5171\u4eab\u8bc1\u636e\u7684\u5206\u914d\u65b9\u5411\u4e0e\u5b83\u4eec\u63d0\u4f9b\u7684\u4fe1\u606f\u4e00\u81f4\u3002\u8bfb\u8005\u53ef\u4ee5\u7528\u4e94\u5341\u4e94\u4e0e\u56db\u5341\u4e94\u7684\u6bd4\u4f8b\u7406\u89e3\u672c\u6b21\u7ed3\u679c\uff0c\u4f46\u4e0d\u80fd\u628a\u8fd9\u4e2a\u7b80\u5316\u7b97\u5f0f\u5f53\u6210 RSEM \u5728\u6240\u6709\u6570\u636e\u4e0a\u7684\u5b8c\u6574\u7b97\u6cd5\u3002\u771f\u5b9e\u8f93\u5165\u8fd8\u6d89\u53ca\u7247\u6bb5\u957f\u5ea6\u3001\u6709\u6548\u957f\u5ea6\u3001\u5339\u914d\u8d28\u91cf\u548c\u6a21\u578b\u4f30\u8ba1\u3002<\/p>\n
\u5c0f\u6570\u8ba1\u6570\u4e0d\u662f\u6587\u4ef6\u683c\u5f0f\u9519\u8bef\uff0c\u4e5f\u4e0d\u5e94\u8be5\u4e3a\u4e86\u770b\u8d77\u6765\u50cf\u6d4b\u5e8f\u6761\u6570\u5c31\u76f4\u63a5\u56db\u820d\u4e94\u5165\u3002\u5b83\u4fdd\u7559\u4e86\u6a21\u578b\u5206\u914d\u7684\u4fe1\u606f\u3002\u8fdb\u5165\u4e0b\u6e38\u5206\u6790\u65f6\uff0c\u5e94\u4f7f\u7528\u8be5\u65b9\u6cd5\u652f\u6301\u7684\u5bfc\u5165\u65b9\u5f0f\uff0c\u5e76\u660e\u786e\u5f53\u524d\u5904\u7406\u7684\u662f\u671f\u671b\u8ba1\u6570\u8fd8\u662f\u76f8\u5bf9\u4e30\u5ea6\u3002\u628a\u4e24\u7c7b\u6570\u503c\u6df7\u5728\u4e00\u8d77\uff0c\u5f80\u5f80\u6bd4\u67d0\u4e00\u4e2a\u53c2\u6570\u9009\u62e9\u66f4\u96be\u8865\u6551\u3002<\/p>\n
\u4e3a\u4ec0\u4e48\u4e0d\u63d0\u4f9b\u5206\u7ec4\u8868\u540e\u5168\u90e8\u53d8\u6210\u767e\u5206\u4e4b\u767e<\/h2>\n
\u7b2c\u4e8c\u7ec4\u4e2d\uff0cA\u3001B\u3001C \u7684\u671f\u671b\u8ba1\u6570\u548c TPM \u70b9\u4f30\u8ba1\u4e0e\u7b2c\u4e00\u7ec4\u76f8\u540c\uff0c\u4f46\u4e09\u6761\u8f6c\u5f55\u672c\u7684\u57fa\u56e0\u5185\u6bd4\u4f8b\u5168\u90e8\u53d8\u4e3a\u767e\u5206\u4e4b\u767e\u3002\u539f\u56e0\u5f88\u76f4\u63a5\uff0c\u6bcf\u6761\u8f6c\u5f55\u672c\u73b0\u5728\u90fd\u88ab\u5f53\u6210\u4e00\u4e2a\u72ec\u7acb\u57fa\u56e0\uff0c\u5b83\u5728\u81ea\u5df1\u7684\u96c6\u5408\u91cc\u5c31\u662f\u552f\u4e00\u6210\u5458\u3002<\/p>\n
\u56e0\u6b64\uff0c\u57fa\u56e0\u5185\u6bd4\u4f8b\u4ece\u4e94\u5341\u4e94\u53d8\u6210\u4e00\u767e\uff0c\u4e0d\u80fd\u89e3\u91ca\u6210\u8be5\u5f02\u6784\u4f53\u8868\u8fbe\u7ffb\u500d\uff0c\u66f4\u4e0d\u80fd\u89e3\u91ca\u6210\u526a\u63a5\u504f\u597d\u6539\u53d8\u3002\u8fd9\u6b21\u8bfb\u6bb5\u6839\u672c\u6ca1\u6709\u53d8\u5316\uff0c\u6539\u53d8\u7684\u53ea\u662f\u5206\u6bcd\u5bf9\u5e94\u7684\u6210\u5458\u96c6\u5408\u3002\u6bd4\u4f8b\u5217\u9700\u8981\u8fde\u540c\u57fa\u56e0\u7f16\u53f7\u3001\u8f6c\u5f55\u672c\u7f16\u53f7\u548c\u6ce8\u91ca\u7248\u672c\u4e00\u8d77\u9605\u8bfb\u3002<\/p>\n
\u5b9e\u9645\u9879\u76ee\u5408\u5e76\u4e0d\u540c\u6765\u6e90\u7684\u53c2\u8003\u65f6\uff0c\u4e5f\u5bb9\u6613\u53d1\u751f\u7c7b\u4f3c\u95ee\u9898\u3002\u67d0\u4e9b\u6761\u76ee\u88ab\u9519\u8bef\u62c6\u6210\u72ec\u7acb\u57fa\u56e0\uff0c\u53e6\u4e00\u4e9b\u6761\u76ee\u4ecd\u7136\u6309\u6b63\u786e\u5173\u7cfb\u5f52\u7ec4\uff0c\u6700\u540e\u4f1a\u5f97\u5230\u770b\u4f3c\u5408\u7406\u5374\u4e0d\u53ef\u6bd4\u8f83\u7684\u6bd4\u4f8b\u3002\u53ea\u67e5\u770b\u8ba1\u6570\u662f\u5426\u4e3a\u6b63\u503c\u65e0\u6cd5\u53d1\u73b0\u8fd9\u4e00\u7c7b\u9519\u8bef\uff0c\u5e94\u62bd\u67e5\u4e00\u4e2a\u660e\u786e\u5177\u6709\u591a\u4e2a\u5f02\u6784\u4f53\u7684\u57fa\u56e0\uff0c\u786e\u8ba4\u5b83\u4eec\u5728\u7ed3\u679c\u4e2d\u786e\u5b9e\u5c5e\u4e8e\u540c\u4e00\u7ec4\u3002<\/p>\n
\u57fa\u56e0\u53ef\u4fe1\u533a\u95f4\u4e0d\u80fd\u76f8\u52a0\u8f6c\u5f55\u672c\u7aef\u70b9\u5f97\u5230<\/h2>\n
\u5206\u7ec4\u53c2\u8003\u4e0b\uff0c\u57fa\u56e0 AB \u7684 TPM \u70b9\u4f30\u8ba1\u4e3a\u516d\u5341\u4e07\uff0c\u5176\u9ed8\u8ba4\u767e\u5206\u4e4b\u4e5d\u5341\u4e94\u53ef\u4fe1\u533a\u95f4\u7ea6\u4e3a\u4e94\u5341\u516d\u70b9\u4e5d\u4e07\u5230\u516d\u5341\u4e8c\u70b9\u4e5d\u4e07\u3002A \u7684\u533a\u95f4\u7ea6\u4e3a\u4e8c\u5341\u516b\u70b9\u516d\u4e07\u5230\u4e09\u5341\u4e03\u70b9\u4e94\u4e07\uff0cB \u7ea6\u4e3a\u4e8c\u5341\u4e8c\u70b9\u516b\u4e07\u5230\u4e09\u5341\u4e00\u70b9\u4e94\u4e07\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u5bf9\u8c61<\/th>\n\u533a\u95f4\u4e0b\u9650<\/th>\n\u533a\u95f4\u4e0a\u9650<\/th>\n<\/tr>\n<\/thead>\n
A<\/td>\n285634<\/td>\n375097<\/td>\n<\/tr>\n
B<\/td>\n227816<\/td>\n315210<\/td>\n<\/tr>\n
\u57fa\u56e0 AB<\/td>\n569039<\/td>\n629692<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u628a A \u4e0e B \u7684\u4e0b\u9650\u76f4\u63a5\u76f8\u52a0\u5f97\u5230\u4e94\u5341\u4e00\u4e07\u4e09\u5343\u56db\u767e\u4e94\u5341\uff0c\u4e0a\u9650\u76f8\u52a0\u5f97\u5230\u516d\u5341\u4e5d\u4e07\u96f6\u4e09\u767e\u96f6\u4e03\uff0c\u90fd\u4e0d\u662f\u7a0b\u5e8f\u8f93\u51fa\u7684\u57fa\u56e0\u533a\u95f4\u3002\u5171\u4eab\u7247\u6bb5\u5728 A \u4e0e B \u4e4b\u95f4\u53d1\u751f\u7684\u5206\u914d\u6ce2\u52a8\u5177\u6709\u8054\u7cfb\uff0c\u4e00\u4e2a\u76ee\u6807\u591a\u5206\u4e00\u4e9b\uff0c\u53e6\u4e00\u4e2a\u5c31\u53ef\u80fd\u5c11\u5206\u4e00\u4e9b\u3002\u6c47\u603b\u65f6\u5e94\u8003\u8651\u8054\u5408\u53d8\u5316\uff0c\u800c\u4e0d\u662f\u628a\u5404\u81ea\u6700\u6781\u7aef\u7684\u7aef\u70b9\u673a\u68b0\u62fc\u63a5\u3002<\/p>\n
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