{"id":73037,"date":"2026-11-15T13:25:50","date_gmt":"2026-11-15T05:25:50","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73037"},"modified":"2026-11-15T13:28:50","modified_gmt":"2026-11-15T05:28:50","slug":"stringtie-guided-assembly-reference-only-exon-skipping-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/stringtie-guided-assembly-reference-only-exon-skipping-practice\/","title":{"rendered":"StringTie \u5b9e\u8dd1\uff0c\u53c2\u8003\u5f15\u5bfc\u7ec4\u88c5\u4e0e\u53ea\u6309\u6ce8\u91ca\u5b9a\u91cf\u7a76\u7adf\u6709\u4ec0\u4e48\u4e0d\u540c"},"content":{"rendered":"
\n

\u540c\u4e00\u4e2a RNA-seq \u6bd4\u5bf9\u6587\u4ef6\uff0c\u4e00\u6b21\u5206\u6790\u62a5\u544a\u4e24\u4e2a\u8f6c\u5f55\u672c\uff0c\u53e6\u4e00\u6b21\u53ea\u62a5\u544a\u4e00\u4e2a\uff0c\u662f\u5426\u8bf4\u660e\u5176\u4e2d\u4e00\u6b21\u4e22\u5931\u4e86\u6570\u636e\uff1f\u5bf9\u4e8e StringTie\uff0c\u9996\u5148\u5e94\u68c0\u67e5\u5206\u6790\u4efb\u52a1\u672c\u8eab\u3002\u4f7f\u7528\u53c2\u8003\u6ce8\u91ca\u5f15\u5bfc\u7ec4\u88c5\uff0c\u4e0e\u9650\u5236\u5728\u7ed9\u5b9a\u6ce8\u91ca\u4e2d\u4f30\u8ba1\u8868\u8fbe\uff0c\u662f\u4e24\u79cd\u4e0d\u540c\u7684\u95ee\u9898\u3002\u540e\u8005\u6ca1\u6709\u8f93\u51fa\u65b0\u7ed3\u6784\uff0c\u5e76\u4e0d\u80fd\u8bc1\u660e\u6837\u672c\u4e0d\u5b58\u5728\u8fd9\u79cd\u7ed3\u6784\u3002<\/p>\n

\u672c\u7bc7\u5b9e\u9645\u5b89\u88c5 StringTie\uff0c\u6784\u9020\u4e00\u4e2a\u5305\u542b\u5916\u663e\u5b50\u8df3\u8dc3\u7684\u4eba\u5de5\u8f6c\u5f55\u533a\u57df\uff0c\u751f\u6210\u8bfb\u6bb5\u5e76\u5b8c\u6210\u526a\u63a5\u6bd4\u5bf9\u3001\u5750\u6807\u6392\u5e8f\u548c\u4e09\u7ec4\u5206\u6790\u3002\u6211\u4eec\u53ea\u6539\u53d8\u662f\u5426\u9650\u5236\u53c2\u8003\u6a21\u578b\uff0c\u4ee5\u53ca\u53c2\u8003\u4e2d\u5305\u542b\u54ea\u4e9b\u8f6c\u5f55\u672c\uff0c\u89c2\u5bdf\u7ed3\u6784\u548c\u4e30\u5ea6\u600e\u6837\u53d8\u5316\u3002\u6240\u6709\u7ed3\u679c\u6765\u81ea\u5b9e\u9645\u8fd0\u884c\uff0c\u4eba\u5de5\u8bbe\u8ba1\u7528\u4e8e\u89e3\u91ca\u53c2\u6570\uff0c\u4e0d\u7528\u4e8e\u5ba3\u79f0\u771f\u5b9e\u6837\u672c\u4e2d\u7684\u53d1\u73b0\u7387\u6216\u5b9a\u91cf\u51c6\u786e\u7387\u3002<\/p>\n

\"\u53ea\u8f93\u51fa\u4e00\u6761\uff0c\u4e0d\u7b49\u4e8e\u53ea\u6709\u4e00\u6761\u3002\u540c\u4e00\u4efd\u6bd4\u5bf9\u6587\u4ef6\uff0c\u4e09\u7ec4\u6a21\u578b\u8303\u56f4\u5bf9\u7167\u3002-G\uff0c\u53c2\u8003\u5f15\u5bfc\u7ec4\u88c5\u5f97\u5230\u4e24\u6761\uff0c\u5df2\u77e5\u7ed3\u6784\u4e0e\u5916\u663e\u5b50\u8df3\u8dc3\u7ed3\u6784\u540c\u65f6\u51fa\u73b0\u3002-G
\u540c\u4e00\u4efd\u6bd4\u5bf9\u6587\u4ef6\uff0c\u4e09\u7ec4\u6a21\u578b\u8303\u56f4\u5bf9\u7167<\/figcaption><\/figure>\n

\u5148\u628a\u7814\u7a76\u95ee\u9898\u5199\u6210\u4e24\u53e5\u8bdd<\/h2>\n

\u5982\u679c\u95ee\u9898\u662f\u6837\u672c\u91cc\u53ef\u80fd\u5b58\u5728\u54ea\u4e9b\u8f6c\u5f55\u7ed3\u6784\uff0c\u5e94\u5141\u8bb8\u7a0b\u5e8f\u7ed3\u5408\u6bd4\u5bf9\u8bc1\u636e\u548c\u53c2\u8003\u6ce8\u91ca\u7ec4\u88c5\u5019\u9009\u6a21\u578b\u3002\u53c2\u8003\u63d0\u4f9b\u5df2\u6709\u7ed3\u6784\u4fe1\u606f\uff0c\u8bfb\u6bb5\u4ecd\u7136\u53ef\u80fd\u652f\u6301\u53c2\u8003\u4e4b\u5916\u7684\u8fde\u63a5\u65b9\u5f0f\u3002\u5982\u679c\u95ee\u9898\u662f\u8fd9\u4e9b\u65e2\u5b9a\u8f6c\u5f55\u672c\u5728\u5404\u6837\u672c\u4e2d\u7684\u8868\u8fbe\u5982\u4f55\uff0c\u5219\u9700\u8981\u56fa\u5b9a\u6a21\u578b\u96c6\u5408\uff0c\u5728\u540c\u4e00\u5957\u5750\u6807\u4e0e\u6807\u8bc6\u4e0b\u4f30\u8ba1\u3002<\/p>\n

StringTie \u7684 -G<\/code> \u63d0\u4f9b\u53c2\u8003\u6ce8\u91ca\u3002\u5355\u72ec\u4f7f\u7528\u65f6\uff0c\u5b83\u53ef\u4ee5\u5e2e\u52a9\u7ec4\u88c5\u5df2\u77e5\u548c\u65b0\u7ed3\u6784\u3002\u52a0\u5165 -e<\/code> \u540e\uff0c\u7a0b\u5e8f\u8fdb\u5165\u9488\u5bf9\u7ed9\u5b9a\u53c2\u8003\u7684\u8868\u8fbe\u4f30\u8ba1\u6a21\u5f0f\uff0c\u9700\u8981\u540c\u65f6\u63d0\u4f9b\u53c2\u8003\u6ce8\u91ca\u3002\u8fd9\u91cc\u7684\u5dee\u522b\u5f71\u54cd\u5019\u9009\u6a21\u578b\u8303\u56f4\uff0c\u4e0d\u80fd\u4ec5\u628a\u5b83\u7406\u89e3\u6210\u63d0\u9ad8\u901f\u5ea6\u7684\u5f00\u5173\u3002StringTie \u5b98\u65b9\u8fd0\u884c\u8bf4\u660e<\/a><\/p>\n

\u8fd9\u79cd\u533a\u5206\u5728\u591a\u6837\u672c\u9879\u76ee\u4e2d\u5f88\u91cd\u8981\u3002\u5404\u6837\u672c\u5148\u5206\u522b\u7ec4\u88c5\uff0c\u53ef\u80fd\u5f97\u5230\u4e0d\u4e00\u81f4\u7684\u8fb9\u754c\u548c\u4e34\u65f6\u7f16\u53f7\u3002\u5982\u679c\u76f4\u63a5\u628a\u540c\u540d\u4e34\u65f6\u8f6c\u5f55\u672c\u653e\u5728\u540c\u4e00\u884c\uff0c\u5c31\u53ef\u80fd\u628a\u4e0d\u540c\u7ed3\u6784\u62fc\u6210\u4e00\u5f20\u77e9\u9635\u3002\u6bd4\u8f83\u8868\u8fbe\u4e4b\u524d\uff0c\u5e94\u5148\u5efa\u7acb\u9002\u5408\u7814\u7a76\u76ee\u7684\u7684\u7edf\u4e00\u6a21\u578b\u96c6\u5408\uff0c\u518d\u8ba9\u5404\u6837\u672c\u5728\u76f8\u540c\u96c6\u5408\u4e0a\u5b9a\u91cf\u3002<\/p>\n

\u8bbe\u8ba1\u4e00\u4e2a\u80fd\u9010\u6bb5\u6838\u5bf9\u7684\u5916\u663e\u5b50\u8df3\u8dc3<\/h2>\n

\u6211\u4eec\u751f\u6210\u4e00\u6761\u516d\u5343\u78b1\u57fa\u7684\u4eba\u5de5\u67d3\u8272\u4f53\uff0c\u5728\u6b63\u94fe\u8bbe\u7f6e\u4e09\u4e2a\u5916\u663e\u5b50\u3002\u5df2\u77e5\u8f6c\u5f55\u672c\u5305\u542b\u4e09\u4e2a\u5916\u663e\u5b50\uff0c\u53e6\u4e00\u6761\u8f6c\u5f55\u672c\u8df3\u8fc7\u4e2d\u95f4\u5916\u663e\u5b50\uff0c\u53ea\u8fde\u63a5\u7b2c\u4e00\u6bb5\u4e0e\u7b2c\u4e09\u6bb5\u3002\u4e24\u4e2a\u8f6c\u5f55\u672c\u7684\u9996\u5c3e\u5750\u6807\u76f8\u540c\uff0c\u5185\u90e8\u7ed3\u6784\u4e0d\u540c\uff0c\u6b63\u597d\u8bf4\u660e\u4e3a\u4ec0\u4e48\u4e0d\u80fd\u53ea\u6309\u8d77\u6b62\u4f4d\u7f6e\u5224\u65ad\u8f6c\u5f55\u672c\u662f\u5426\u4e00\u81f4\u3002<\/p>\n

\n\n\n\n\n\n\n
\u6a21\u578b<\/th>\n\u5916\u663e\u5b50\u5750\u6807<\/th>\n<\/tr>\n<\/thead>\n
\u5df2\u77e5<\/td>\n1001\u20131400\u30012501\u20132700\u30014001\u20134400<\/td>\n<\/tr>\n
\u8df3\u8dc3<\/td>\n1001\u20131400\u30014001\u20134400<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u4e24\u8005\u7684\u526a\u63a5\u540e\u5e8f\u5217\u5206\u522b\u957f\u4e00\u5343\u548c\u516b\u767e\u78b1\u57fa\u3002<\/p>\n

\u521d\u59cb\u53c2\u8003\u53ea\u5199\u5165\u4e09\u5916\u663e\u5b50\u7684\u5df2\u77e5\u6a21\u578b\u3002\u53e6\u51c6\u5907\u4e00\u4efd\u5b8c\u6574\u53c2\u8003\uff0c\u540c\u65f6\u5199\u5165\u5df2\u77e5\u6a21\u578b\u548c\u8df3\u8dc3\u6a21\u578b\u3002\u6ce8\u610f\uff0c\u5b8c\u6574\u53c2\u8003\u4e4b\u6240\u4ee5\u77e5\u9053\u8df3\u8dc3\u7ed3\u6784\uff0c\u662f\u56e0\u4e3a\u8fd9\u662f\u6211\u4eec\u8bbe\u8ba1\u7684\u5bf9\u7167\uff0c\u4e0d\u4ee3\u8868\u771f\u5b9e\u9879\u76ee\u53ef\u4ee5\u63d0\u524d\u77e5\u9053\u6240\u6709\u65b0\u8f6c\u5f55\u672c\u3002<\/p>\n

\u6839\u636e\u4e24\u6761\u526a\u63a5\u540e\u7684\u5e8f\u5217\uff0c\u811a\u672c\u4ee5\u56fa\u5b9a\u95f4\u9694\u53d6\u4e00\u767e\u4e94\u5341\u78b1\u57fa\u7684\u5355\u7aef\u8bfb\u6bb5\uff0c\u6bcf\u4e2a\u4f4d\u7f6e\u751f\u6210\u4e09\u4efd\uff0c\u7136\u540e\u6253\u4e71\u987a\u5e8f\u3002\u5df2\u77e5\u6a21\u578b\u8d21\u732e\u4e00\u5343\u4e8c\u767e\u4e03\u5341\u516b\u6761\uff0c\u8df3\u8dc3\u6a21\u578b\u8d21\u732e\u4e5d\u767e\u4e03\u5341\u516b\u6761\uff0c\u603b\u8ba1\u4e8c\u5343\u4e8c\u767e\u4e94\u5341\u516d\u6761\u3002\u5e8f\u5217\u65e0\u6d4b\u5e8f\u9519\u8bef\uff0c\u8d28\u91cf\u503c\u4e00\u81f4\uff0c\u8986\u76d6\u4e5f\u53d7\u4eba\u4e3a\u53d6\u6837\u65b9\u5f0f\u5f71\u54cd\u3002\u8fd9\u4e9b\u6761\u4ef6\u4fbf\u4e8e\u6838\u5bf9\u7ed3\u6784\uff0c\u4e0d\u80fd\u4ee3\u66ff\u6709\u6279\u6b21\u5dee\u5f02\u548c\u771f\u5b9e\u566a\u58f0\u7684\u751f\u7269\u5b66\u91cd\u590d\u3002<\/p>\n

\u5b89\u88c5\u540e\u5148\u786e\u8ba4\u8f93\u5165\u5750\u6807\u4f53\u7cfb<\/h2>\n

\u672c\u6b21 StringTie \u5b9e\u9645\u7248\u672c\u4e3a\u4e09\u70b9\u96f6\u70b9\u4e09\uff0cHISAT2 \u4e3a\u4e8c\u70b9\u4e8c\u70b9\u4e09\uff0c\u6392\u5e8f\u4f7f\u7528 Samtools \u4e00\u70b9\u4e8c\u5341\u4e8c\u70b9\u4e00\u3002\u590d\u73b0\u811a\u672c\u9501\u5b9a\u4e09\u4e2a\u5bb9\u5668\u6458\u8981\uff0c\u5e76\u8bb0\u5f55\u53c2\u8003\u5e8f\u5217\u6458\u8981\u3001\u8bfb\u6bb5\u6765\u6e90\u548c\u5b9e\u9645\u547d\u4ee4\u3002\u8bfb\u8005\u53ef\u4ee5\u4ece\u516c\u5f00\u9644\u4ef6\u751f\u6210\u540c\u6837\u7684\u8f93\u5165\uff0c\u518d\u5b8c\u6574\u6267\u884c\u4e09\u7ec4\u5bf9\u7167\u3002<\/p>\n

docker pull quay.io\/biocontainers\/stringtie:3.0.3--h29c0135_0\r\ndocker run --rm quay.io\/biocontainers\/stringtie:3.0.3--h29c0135_0 \\\r\n  stringtie --version\r\n\r\n# \u89e3\u538b\u590d\u73b0\u5305\u540e\uff0c\u5728\u5bf9\u5e94\u5de5\u5177\u76ee\u5f55\u6267\u884c\r\npython3 run.py .\/practice\r\n<\/code><\/pre>\n
StringTie \u4f7f\u7528\u6309\u57fa\u56e0\u7ec4\u5750\u6807\u6392\u5217\u7684 RNA \u6bd4\u5bf9\u7ed3\u679c\u3002\u672c\u6b21\u5148\u7528 HISAT2 \u5c06\u8bfb\u6bb5\u6bd4\u5bf9\u5230\u4eba\u5de5\u67d3\u8272\u4f53\uff0c\u518d\u7528 Samtools \u6392\u5e8f\u3002\u4e0d\u80fd\u62ff\u5230\u4e00\u4e2a\u6587\u4ef6\u6269\u5c55\u540d\u4e3a BAM \u7684\u7ed3\u679c\u5c31\u76f4\u63a5\u8ba4\u4e3a\u9002\u7528\uff0c\u8f6c\u5f55\u672c\u5750\u6807\u4e0a\u7684\u6bd4\u5bf9\u4e0e\u57fa\u56e0\u7ec4\u5750\u6807\u4e0a\u7684\u526a\u63a5\u6bd4\u5bf9\u56de\u7b54\u7684\u662f\u4e0d\u540c\u95ee\u9898\u3002\u53c2\u8003\u5e8f\u5217\u540d\u548c\u6ce8\u91ca\u5750\u6807\u4e5f\u5fc5\u987b\u5339\u914d\u3002<\/p>\n
hisat2_extract_splice_sites.py known.gtf > known.ss\r\nhisat2-build -p 2 genome.fa genome\r\nhisat2 -p 2 --rna-strandness F \\\r\n  --known-splicesite-infile known.ss --seed 146 \\\r\n  -x genome -U reads.fq -S reads.sam\r\nsamtools sort -@ 1 -o reads.bam reads.sam\r\nsamtools quickcheck -v reads.bam\r\n<\/code><\/pre>\n
\u4e0a\u9762\u547d\u4ee4\u4e2d\u7684\u94fe\u65b9\u5411\u6765\u81ea\u4eba\u5de5\u8bfb\u6bb5\u7684\u751f\u6210\u65b9\u5f0f\uff0c\u4e0d\u662f\u6240\u6709\u5b9e\u9a8c\u90fd\u5e94\u4f7f\u7528\u7684\u9ed8\u8ba4\u7b54\u6848\u3002\u771f\u5b9e\u9879\u76ee\u5e94\u6838\u5bf9\u6587\u5e93\u65b9\u5411\uff0c\u4ee5\u53ca\u526a\u63a5\u6bd4\u5bf9\u4e2d\u6709\u5173\u8f6c\u5f55\u65b9\u5411\u7684\u6807\u8bb0\u3002\u6392\u5e8f\u68c0\u67e5\u80fd\u591f\u6392\u9664\u4e00\u90e8\u5206\u6587\u4ef6\u95ee\u9898\uff0c\u4f46\u65e0\u6cd5\u8bc1\u660e\u8bfb\u6bb5\u5df2\u7ecf\u6b63\u786e\u652f\u6301\u4e86\u6bcf\u4e00\u4e2a\u5019\u9009\u7ed3\u6784\u3002<\/p>\n
\u4e09\u7ec4\u5206\u6790\u53ea\u6539\u53d8\u6a21\u578b\u8303\u56f4<\/h2>\n
\u4e09\u7ec4 StringTie \u547d\u4ee4\u4f7f\u7528\u540c\u4e00\u4efd\u6392\u5e8f\u6587\u4ef6\uff0c\u5747\u4fdd\u7559\u7aef\u90e8\u4f4d\u7f6e\u3001\u4e0d\u6309\u8986\u76d6\u8870\u51cf\u4fee\u526a\uff0c\u4ee5\u4fbf\u6bd4\u8f83\u8bbe\u8ba1\u8fb9\u754c\u3002\u7b2c\u4e00\u7ec4\u7528\u65e7\u53c2\u8003\u5f15\u5bfc\u7ec4\u88c5\uff0c\u7b2c\u4e8c\u7ec4\u5728\u65e7\u53c2\u8003\u4e0a\u9650\u5236\u5b9a\u91cf\uff0c\u7b2c\u4e09\u7ec4\u5728\u5305\u542b\u4e24\u6761\u8f6c\u5f55\u672c\u7684\u5b8c\u6574\u53c2\u8003\u4e0a\u9650\u5236\u5b9a\u91cf\u3002<\/p>\n
stringtie reads.bam -p 2 --fr -t \\\r\n  -G known.gtf -o guided.gtf -A guided.genes.tsv\r\n\r\nstringtie reads.bam -p 2 --fr -t -e \\\r\n  -G known.gtf -o known_only.gtf -A known_only.genes.tsv\r\n\r\nstringtie reads.bam -p 2 --fr -t -e \\\r\n  -G union.gtf -o union_only.gtf -A union_only.genes.tsv\r\n<\/code><\/pre>\n
\u8fd9\u91cc\u5173\u95ed\u7aef\u90e8\u4fee\u526a\u662f\u4e3a\u4e86\u51cf\u5c11\u672c\u6b21\u5bf9\u7167\u4e2d\u7684\u989d\u5916\u53d8\u91cf\uff0c\u4e0d\u5e94\u63a8\u5e7f\u6210\u6240\u6709\u6837\u672c\u90fd\u5fc5\u987b\u7167\u642c\u7684\u53c2\u6570\u3002\u5bf9\u4e8e\u8986\u76d6\u6e10\u964d\u3001\u964d\u89e3\u660e\u663e\u6216\u8005\u7aef\u90e8\u8bc1\u636e\u4e0d\u8db3\u7684\u6570\u636e\uff0c\u8fb9\u754c\u672c\u6765\u5c31\u9700\u8981\u7ed3\u5408\u8bfb\u6bb5\u5206\u5e03\u548c\u5176\u4ed6\u8bc1\u636e\u5224\u65ad\u3002\u672c\u7bc7\u53ea\u68c0\u9a8c\u5916\u663e\u5b50\u8fde\u63a5\u65b9\u5f0f\u548c\u53c2\u8003\u96c6\u5408\u5bf9\u8f93\u51fa\u7684\u5f71\u54cd\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u5206\u6790\u65b9\u5f0f<\/th>\n\u7ed3\u6784\u6570<\/th>\n\u8df3\u8dc3\u7ed3\u6784<\/th>\n<\/tr>\n<\/thead>\n
\u5f15\u5bfc\u7ec4\u88c5<\/td>\n2<\/td>\n\u7ec4\u88c5\u5f97\u5230<\/td>\n<\/tr>\n
\u65e7\u53c2\u8003\u5b9a\u91cf<\/td>\n1<\/td>\n\u672a\u8f93\u51fa<\/td>\n<\/tr>\n
\u53cc\u6a21\u578b\u5b9a\u91cf<\/td>\n2<\/td>\n\u53c2\u8003\u5185\u5b9a\u91cf<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u7b2c\u4e00\u7ec4\u8f93\u51fa\u7684\u4e24\u6761\u6a21\u578b\uff0c\u5916\u663e\u5b50\u5750\u6807\u5206\u522b\u4e0e\u8bbe\u8ba1\u7684\u4e09\u5916\u663e\u5b50\u548c\u4e24\u5916\u663e\u5b50\u7ed3\u6784\u4e00\u81f4\u3002\u7b2c\u4e8c\u7ec4\u53ea\u7559\u4e0b\u5df2\u77e5\u6a21\u578b\u3002\u7b2c\u4e09\u7ec4\u6062\u590d\u4e24\u6761\u6a21\u578b\uff0c\u4f46\u5b83\u4eec\u4f5c\u4e3a\u53c2\u8003\u4e2d\u7684\u6761\u76ee\u88ab\u4f30\u8ba1\u3002\u4e09\u4e2a\u7ed3\u679c\u4e0d\u662f\u7b80\u5355\u7684\u6b63\u786e\u4e0e\u9519\u8bef\u4e4b\u5206\uff0c\u800c\u662f\u4e0d\u540c\u4efb\u52a1\u8fb9\u754c\u7684\u76f4\u63a5\u8868\u73b0\u3002<\/p>\n
\u5224\u65ad\u4e00\u4e2a\u65b0\u7ed3\u6784\uff0c\u8981\u770b\u8fde\u63a5\u8bc1\u636e<\/h2>\n
\u6b64\u6b21\u4e24\u5343\u591a\u6761\u8bfb\u6bb5\u5168\u90e8\u6210\u529f\u4e14\u552f\u4e00\u6bd4\u5bf9\uff0c\u4f46\u8fd9\u8fd8\u4e0d\u591f\u3002\u89e3\u6790\u5b9e\u9645\u6bd4\u5bf9\u4e2d\u7684\u8df3\u8dc3\u64cd\u4f5c\u540e\uff0c\u5df2\u77e5\u7ed3\u6784\u7684\u4e24\u4e2a\u5185\u542b\u5b50\u5206\u522b\u5f97\u5230\u4e8c\u767e\u4e09\u5341\u4e94\u6b21\u548c\u4e8c\u767e\u4e09\u5341\u516d\u6b21\u652f\u6301\uff0c\u8df3\u8dc3\u7ed3\u6784\u8de8\u8d8a\u7684\u957f\u5185\u542b\u5b50\u5f97\u5230\u4e00\u767e\u4e5d\u5341\u4e94\u6b21\u652f\u6301\u3002\u8fd9\u4e9b\u6570\u5b57\u8bf4\u660e\uff0c\u8df3\u8dc3\u8fde\u63a5\u6709\u72ec\u7acb\u7684\u526a\u63a5\u6bd4\u5bf9\u8bc1\u636e\uff0c\u800c\u4e0d\u662f\u53ea\u56e0\u4e3a\u4e00\u4e2a\u8f93\u51fa\u6587\u4ef6\u91cc\u591a\u4e86\u4e00\u884c\u3002<\/p>\n
\u5bf9\u5e94\u7684\u5185\u542b\u5b50\u95ed\u533a\u95f4\u5206\u522b\u4e3a\u4e00\u5343\u56db\u767e\u96f6\u4e00\u5230\u4e8c\u5343\u4e94\u767e\u3001\u4e8c\u5343\u4e03\u767e\u96f6\u4e00\u5230\u56db\u5343\uff0c\u4ee5\u53ca\u4e00\u5343\u56db\u767e\u96f6\u4e00\u5230\u56db\u5343\u3002\u6700\u540e\u4e00\u6bb5\u8de8\u8fc7\u4e86\u539f\u6765\u7684\u4e2d\u95f4\u5916\u663e\u5b50\u3002\u68c0\u67e5\u8fd9\u79cd\u5173\u7cfb\u65f6\uff0c\u6700\u597d\u540c\u65f6\u5199\u51fa\u524d\u4e00\u5916\u663e\u5b50\u672b\u7aef\u3001\u540e\u4e00\u5916\u663e\u5b50\u8d77\u70b9\u548c\u4e2d\u95f4\u95f4\u9694\uff0c\u907f\u514d\u628a\u5185\u542b\u5b50\u957f\u5ea6\u4e0e\u5750\u6807\u7aef\u70b9\u6df7\u5728\u4e00\u8d77\u3002<\/p>\n
\u5b9e\u9645\u6bd4\u5bf9\u8fd8\u51fa\u73b0\u4e8c\u5341\u4e03\u4e2a\u8f6f\u526a\u5207\u64cd\u4f5c\u3002\u5373\u4f7f\u603b\u4f53\u6bd4\u5bf9\u7387\u8fbe\u5230\u767e\u5206\u4e4b\u767e\uff0c\u4e5f\u4e0d\u80fd\u636e\u6b64\u8bf4\u6bcf\u6761\u8bfb\u6bb5\u90fd\u5b8c\u6574\u652f\u6301\u4e86\u9884\u671f\u526a\u63a5\u3002\u672c\u6587\u4fdd\u7559\u8fd9\u4e00\u73b0\u8c61\uff0c\u5e76\u6ca1\u6709\u5c06\u6bd4\u5bf9\u7387\u5305\u88c5\u6210\u7ed3\u6784\u6b63\u786e\u7387\u3002\u771f\u5b9e\u9879\u76ee\u5e94\u68c0\u67e5\u8de8\u8fde\u63a5\u8bfb\u6bb5\u7684\u4e24\u4fa7\u951a\u5b9a\u957f\u5ea6\u3001\u9519\u914d\u3001\u91cd\u590d\u533a\u57df\u53ca\u5f02\u5e38\u8986\u76d6\uff0c\u800c\u4e0d\u4ec5\u662f\u6d4f\u89c8\u5668\u4e2d\u770b\u8d77\u6765\u5b58\u5728\u4e00\u6761\u8fde\u7ebf\u3002<\/p>\n
\u4eba\u5de5\u5bf9\u7167\u4e2d\u6211\u4eec\u77e5\u9053\u751f\u6210\u771f\u503c\uff0c\u53ef\u4ee5\u786e\u8ba4\u8f93\u51fa\u7ed3\u6784\u4e0e\u8bbe\u8ba1\u4e00\u81f4\u3002\u771f\u5b9e\u6837\u672c\u4e2d\u7684\u65b0\u7ed3\u6784\u5219\u9700\u8981\u66f4\u591a\u8bc1\u636e\uff0c\u5c24\u5176\u662f\u4f4e\u8986\u76d6\u8fde\u63a5\u3001\u5355\u4e00\u6837\u672c\u72ec\u6709\u7ed3\u6784\u548c\u590d\u6742\u540c\u6e90\u533a\u57df\u3002\u5019\u9009\u7ed3\u6784\u53ef\u4ee5\u6210\u4e3a\u540e\u7eed\u9a8c\u8bc1\u5bf9\u8c61\uff0c\u4f46\u4e0d\u80fd\u53ea\u51ed\u7ec4\u88c5\u8f93\u51fa\u5c31\u5ba3\u5e03\u53d1\u73b0\u4e86\u7a33\u5b9a\u8868\u8fbe\u7684\u65b0\u5f02\u6784\u4f53\u3002<\/p>\n
\u4e3a\u4ec0\u4e48\u65e7\u53c2\u8003\u5b9a\u91cf\u5f97\u5230\u4e00\u767e\u4e07 TPM<\/h2>\n
\u65e7\u53c2\u8003\u9650\u5236\u5b9a\u91cf\u7ec4\u53ea\u8f93\u51fa\u4e00\u6761\u8f6c\u5f55\u672c\uff0c\u5176 TPM \u4e3a\u4e00\u767e\u4e07\u3002\u8fd9\u4e2a\u6570\u5b57\u5f88\u5bb9\u6613\u8bef\u5bfc\u8bfb\u8005\uff0c\u4ee5\u4e3a\u6240\u6709\u8bfb\u6bb5\u90fd\u6765\u81ea\u8be5\u8f6c\u5f55\u672c\uff0c\u6216\u8005\u5b83\u5728\u771f\u5b9e\u6837\u672c\u4e2d\u5177\u6709\u6781\u7aef\u9ad8\u8868\u8fbe\u3002\u5b9e\u9645\u4e0a\uff0c\u8fd9\u6b21\u8f93\u51fa\u96c6\u5408\u53ea\u6709\u4e00\u4e2a\u53ef\u62a5\u544a\u7684\u6a21\u578b\uff0c\u76f8\u5bf9\u4e30\u5ea6\u5f52\u4e00\u5316\u7684\u5206\u6bcd\u4e5f\u53d7\u6a21\u578b\u96c6\u5408\u7ea6\u675f\u3002<\/p>\n
\u5141\u8bb8\u4e24\u4e2a\u6a21\u578b\u540e\uff0c\u5df2\u77e5\u8f6c\u5f55\u672c\u7684 TPM \u7ea6\u4e3a\u516d\u5341\u70b9\u516b\u4e07\uff0c\u8df3\u8dc3\u6a21\u578b\u7ea6\u4e3a\u4e09\u5341\u4e5d\u70b9\u4e8c\u4e07\u3002\u5f15\u5bfc\u7ec4\u88c5\u7ec4\u5206\u522b\u4e3a\u516d\u5341\u4e07\u4e03\u5343\u4e5d\u767e\u4e94\u5341\u4e09\u70b9\u4e00\u516b\u548c\u4e09\u5341\u4e5d\u4e07\u4e8c\u5343\u96f6\u56db\u5341\u516d\u70b9\u516b\u4e8c\uff0c\u5b8c\u6574\u53c2\u8003\u5b9a\u91cf\u7ec4\u5206\u522b\u4e3a\u516d\u5341\u4e07\u4e03\u5343\u516b\u767e\u4e94\u5341\u4e00\u70b9\u516b\u4e00\u548c\u4e09\u5341\u4e5d\u4e07\u4e8c\u5343\u4e00\u767e\u56db\u5341\u516b\u70b9\u4e00\u4e5d\u3002\u4e24\u7ec4\u63a5\u8fd1\uff0c\u4f46\u5e76\u975e\u9010\u4f4d\u76f8\u540c\u3002<\/p>\n
\u8fd9\u4e9b\u5dee\u5f02\u4e0d\u5e94\u8be5\u88ab\u76f4\u63a5\u89e3\u91ca\u4e3a\u8868\u8fbe\u53d1\u751f\u4e86\u53d8\u5316\uff0c\u56e0\u4e3a\u8f93\u5165\u6bd4\u5bf9\u6587\u4ef6\u5b8c\u5168\u76f8\u540c\u3002\u6539\u53d8\u7684\u662f\u6a21\u578b\u8303\u56f4\u548c\u4f30\u8ba1\u8fc7\u7a0b\u3002\u65e7\u53c2\u8003\u7ec4\u5c06\u53ef\u89e3\u91ca\u7684\u5171\u4eab\u533a\u57df\u8bc1\u636e\u653e\u5728\u66f4\u53d7\u9650\u7684\u6a21\u578b\u4e2d\uff0c\u7ed3\u679c\u4e5f\u4e0d\u4f1a\u544a\u8bc9\u4f60\u53c2\u8003\u4e4b\u5916\u7684\u7ed3\u6784\u4e0d\u5b58\u5728\u3002\u82e5\u4e00\u4e2a\u6837\u672c\u7528\u65e7\u53c2\u8003\u3001\u53e6\u4e00\u4e2a\u6837\u672c\u7528\u6269\u5145\u53c2\u8003\uff0c\u4e30\u5ea6\u8868\u9762\u4e0a\u7684\u5dee\u5f02\u53ef\u80fd\u9996\u5148\u53cd\u6620\u5206\u6bcd\u548c\u5206\u914d\u65b9\u5f0f\u6539\u53d8\u3002<\/p>\n
\u8fd8\u5e94\u907f\u514d\u62ff\u4eba\u5de5\u751f\u6210\u7684\u8bfb\u6bb5\u4efd\u6570\u76f4\u63a5\u68c0\u9a8c TPM \u6bd4\u4f8b\u3002\u4e24\u6761\u8f6c\u5f55\u672c\u957f\u5ea6\u4e0d\u540c\uff0c\u53d6\u6837\u4f4d\u7f6e\u7684\u6570\u91cf\u4e0d\u540c\uff0c\u8fb9\u7f18\u8986\u76d6\u548c\u5171\u4eab\u533a\u57df\u4e5f\u4e0d\u540c\u3002\u8bfb\u6bb5\u6765\u6e90\u6bd4\u4f8b\u3001\u5e73\u5747\u8986\u76d6\u548c\u6309\u6709\u6548\u4fe1\u606f\u4f30\u8ba1\u7684\u76f8\u5bf9\u4e30\u5ea6\uff0c\u5404\u6709\u81ea\u5df1\u7684\u7edf\u8ba1\u542b\u4e49\u3002\u672c\u6b21\u8bbe\u8ba1\u9002\u5408\u89c2\u5bdf\u53c2\u6570\u9020\u6210\u7684\u53d8\u5316\uff0c\u5e76\u4e0d\u662f\u7ecf\u8fc7\u6821\u51c6\u7684\u8868\u8fbe\u771f\u503c\u57fa\u51c6\u3002<\/p>\n
\u4e34\u65f6\u7f16\u53f7\u76f8\u540c\uff0c\u4e0d\u4ee3\u8868\u8de8\u6837\u672c\u7ed3\u6784\u76f8\u540c<\/h2>\n
\u5f15\u5bfc\u7ec4\u88c5\u7ec4\u4e3a\u4e24\u6761\u8f93\u51fa\u751f\u6210\u4e34\u65f6\u7f16\u53f7\uff0c\u5e76\u7ed9\u5df2\u77e5\u7ed3\u6784\u9644\u4e0a\u53c2\u8003\u5bf9\u5e94\u4fe1\u606f\u3002\u9650\u5236\u5b9a\u91cf\u7ec4\u5219\u4f7f\u7528\u53c2\u8003\u4e2d\u7684\u8f6c\u5f55\u672c\u7f16\u53f7\u3002\u6574\u7406\u7ed3\u679c\u65f6\uff0c\u5e94\u4fdd\u5b58\u53c2\u8003\u5bf9\u5e94\u5c5e\u6027\u548c\u5b8c\u6574\u5916\u663e\u5b50\u94fe\uff0c\u800c\u4e0d\u662f\u53ea\u4fdd\u7559\u4e00\u4e2a\u770b\u8d77\u6765\u7b80\u6d01\u7684\u540d\u79f0\u3002<\/p>\n
\u57fa\u56e0\u4e30\u5ea6\u8868\u4e5f\u9700\u8981\u4ed4\u7ec6\u770b\u3002\u6b64\u6b21\u5f15\u5bfc\u7ec4\u88c5\u8f93\u51fa\u540c\u65f6\u5305\u542b\u53c2\u8003\u57fa\u56e0\u7f16\u53f7\u4e0e\u7ec4\u88c5\u57fa\u56e0\u7f16\u53f7\uff0c\u5b83\u4eec\u4f4d\u4e8e\u540c\u4e00\u6761\u67d3\u8272\u4f53\u3001\u540c\u4e00\u94fe\u548c\u540c\u4e00\u8303\u56f4\u3002\u82e5\u4ec5\u628a\u4e0d\u540c\u7f16\u53f7\u5f53\u6210\u4e0d\u540c\u57fa\u56e0\u76f8\u52a0\uff0c\u5c31\u4f1a\u628a\u4e00\u4e2a\u533a\u57df\u91cd\u590d\u89e3\u91ca\u6210\u4e24\u4e2a\u72ec\u7acb\u5bf9\u8c61\u3002\u8fd9\u4e2a\u73b0\u8c61\u63d0\u9192\u6211\u4eec\u5148\u6838\u5bf9\u8bb0\u5f55\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u518d\u6c47\u603b\u6570\u91cf\u6216\u7ed8\u56fe\u3002<\/p>\n
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