{"id":73095,"date":"2026-11-15T13:42:51","date_gmt":"2026-11-15T05:42:51","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73095"},"modified":"2026-11-15T13:42:51","modified_gmt":"2026-11-15T05:42:51","slug":"flair-splice-correction-junction-support-collapse-filter-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/flair-splice-correction-junction-support-collapse-filter-practice\/","title":{"rendered":"FLAIR \u5b9e\u8dd1\uff0c\u526a\u63a5\u6821\u6b63\u901a\u8fc7\u540e\uff0c\u4e3a\u4ec0\u4e48\u5f02\u6784\u4f53\u4ecd\u4f1a\u88ab\u8fc7\u6ee4"},"content":{"rendered":"
\n

\u957f\u8bfb\u957f RNA \u6570\u636e\u770b\u8d77\u6765\u80fd\u591f\u76f4\u63a5\u8bfb\u51fa\u6574\u6761\u8f6c\u5f55\u672c\uff0c\u4f46\u6bd4\u5bf9\u4f4d\u7f6e\u3001\u526a\u63a5\u8fb9\u754c\u3001\u622a\u77ed\u548c\u652f\u6301\u6570\u91cf\u4ecd\u4f1a\u5f71\u54cd\u6700\u7ec8\u7ed3\u679c\u3002\u4e00\u4e2a\u8bfb\u6bb5\u901a\u8fc7\u526a\u63a5\u6821\u6b63\uff0c\u4e0d\u4ee3\u8868\u5b83\u5bf9\u5e94\u7684\u5f02\u6784\u4f53\u4e00\u5b9a\u8fdb\u5165\u6700\u7ec8\u6a21\u578b\u3002\u53cd\u8fc7\u6765\uff0c\u6700\u7ec8\u6ca1\u6709\u67d0\u4e2a\u6a21\u578b\uff0c\u4e5f\u4e0d\u610f\u5473\u7740\u539f\u59cb\u6570\u636e\u4e2d\u5b8c\u5168\u6ca1\u6709\u76f8\u5173\u8bfb\u6bb5\u3002<\/p>\n

\u672c\u7bc7\u5b9e\u9645\u8fd0\u884c FLAIR \u4e09\u70b9\u96f6\u70b9\u4e00\u7684\u6bd4\u5bf9\u3001\u6821\u6b63\u548c\u5f52\u5e76\u6b65\u9aa4\uff0c\u4f7f\u7528\u4e8c\u5341\u6761\u4eba\u5de5\u957f\u8bfb\u6bb5\uff0c\u5206\u522b\u68c0\u67e5\u6821\u6b63\u7a97\u53e3\u3001\u8865\u5145\u8fde\u63a5\u8868\u548c\u6700\u5c11\u652f\u6301\u6570\u91cf\u3002\u6240\u6709\u8fd0\u884c\u7ed3\u679c\u90fd\u4fdd\u7559\u5230\u9644\u4ef6\u4e2d\u3002\u91cd\u70b9\u662f\u8ffd\u8e2a\u8bc1\u636e\u5728\u54ea\u4e00\u6b65\u88ab\u63a5\u53d7\u6216\u6392\u9664\uff0c\u5e2e\u52a9\u8bfb\u8005\u89e3\u91ca\u6700\u7ec8\u6a21\u578b\u6570\u91cf\u4e3a\u4ec0\u4e48\u53d8\u5316\u3002<\/p>\n

\"\u6821\u6b63\u901a\u8fc7\uff0c\u8fd8\u8981\u6838\u5bf9\u6700\u7ec8\u6a21\u578b\u3002\u4e8c\u5341\u6761\u8bbe\u8ba1\u8bfb\u6bb5\uff0c\u8ffd\u8e2a\u6821\u6b63\u4e0e\u652f\u6301\u95e8\u69db\u300212
\u4e8c\u5341\u6761\u8bbe\u8ba1\u8bfb\u6bb5\uff0c\u8ffd\u8e2a\u6821\u6b63\u4e0e\u652f\u6301\u95e8\u69db<\/figcaption><\/figure>\n

\u628a\u4e09\u4e2a\u9636\u6bb5\u5206\u5f00\u770b<\/h2>\n

\u6bd4\u5bf9\u9636\u6bb5\u628a\u8bfb\u6bb5\u653e\u5230\u57fa\u56e0\u7ec4\u4e0a\uff0c\u5e76\u5f62\u6210\u5305\u542b\u5916\u663e\u5b50\u533a\u6bb5\u7684\u8bb0\u5f55\u3002\u6821\u6b63\u9636\u6bb5\u7ed3\u5408\u6ce8\u91ca\u6216\u8865\u5145\u526a\u63a5\u4f4d\u70b9\u4fe1\u606f\uff0c\u5904\u7406\u504f\u79fb\u7684\u8fb9\u754c\u5e76\u8fc7\u6ee4\u65e0\u6cd5\u6838\u9a8c\u7684\u8bb0\u5f55\u3002\u5f52\u5e76\u9636\u6bb5\u518d\u628a\u76f8\u5bb9\u7684\u8bc1\u636e\u7ec4\u7ec7\u6210\u5f02\u6784\u4f53\u6a21\u578b\uff0c\u5e76\u68c0\u67e5\u652f\u6301\u6570\u91cf\u4e0e\u5176\u4ed6\u89c4\u5219\u3002<\/p>\n

\u56e0\u6b64\uff0c\u6bcf\u4e2a\u9636\u6bb5\u90fd\u6709\u81ea\u5df1\u7684\u8f93\u5165\u5bf9\u8c61\u548c\u8f93\u51fa\u96c6\u5408\u3002\u539f\u59cb\u8bfb\u6bb5\u6570\u3001\u6821\u6b63\u4fdd\u7559\u6570\u3001\u5019\u9009\u6a21\u578b\u6570\u548c\u6700\u7ec8\u6a21\u578b\u6570\u4e0d\u80fd\u4e92\u76f8\u4ee3\u66ff\u3002\u82e5\u5206\u6790\u53ea\u7559\u4e0b\u6700\u540e\u4e00\u5f20\u8868\uff0c\u67d0\u6761\u5019\u9009\u4e3a\u4ec0\u4e48\u6d88\u5931\uff0c\u5f80\u5f80\u5c31\u65e0\u6cd5\u89e3\u91ca\u3002<\/p>\n

FLAIR \u8fd8\u63d0\u4f9b\u5176\u4ed6\u5206\u6790\u8def\u5f84\uff0c\u672c\u6b21\u53ea\u8fd0\u884c\u4e0a\u8ff0\u4e09\u4e2a\u6a21\u5757\uff0c\u6ca1\u6709\u6267\u884c\u5dee\u5f02\u8868\u8fbe\u3001\u878d\u5408\u5206\u6790\u6216\u53e6\u4e00\u5957\u6574\u5408\u6d41\u7a0b\u3002\u6a21\u5757\u8bf4\u660e\u4e2d\u7684\u6027\u80fd\u6bd4\u8f83\u4e5f\u4e0d\u662f\u8fd9\u6b21\u5b9e\u9a8c\u6d4b\u91cf\u7684\u7ed3\u679c\uff0c\u4e0d\u80fd\u76f4\u63a5\u62ff\u6765\u4f5c\u4e3a\u5f53\u524d\u8f93\u5165\u7684\u5b9e\u6d4b\u7ed3\u8bba\u3002FLAIR \u5b98\u65b9\u6a21\u5757\u8bf4\u660e<\/a><\/p>\n

\u4e8c\u5341\u6761\u8bfb\u6bb5\u5206\u522b\u6d4b\u8bd5\u4ec0\u4e48<\/h2>\n

\u4eba\u5de5\u53c2\u8003\u5305\u542b\u4e09\u4e2a\u5916\u663e\u5b50\uff0c\u4e00\u57fa\u5236\u5750\u6807\u5206\u522b\u4e3a\u4e00\u5343\u96f6\u4e00\u5230\u4e00\u5343\u56db\u767e\u3001\u4e8c\u5343\u4e94\u767e\u96f6\u4e00\u5230\u4e8c\u5343\u4e5d\u767e\u3001\u56db\u5343\u96f6\u4e00\u5230\u56db\u5343\u56db\u767e\u3002\u53c2\u8003\u6ce8\u91ca\u53ea\u5199\u5165\u5b8c\u6574\u7684\u4e09\u5916\u663e\u5b50\u6a21\u578b\u3002\u6211\u4eec\u4ece\u8fd9\u4e2a\u533a\u57df\u751f\u6210\u4e09\u7c7b\u65e0\u6d4b\u5e8f\u9519\u8bef\u7684\u957f\u8bfb\u6bb5\u3002<\/p>\n

\u7b2c\u4e00\u7c7b\u5305\u542b\u4e09\u4e2a\u5916\u663e\u5b50\uff0c\u5171\u5341\u4e8c\u6761\u3002\u7b2c\u4e8c\u7c7b\u8df3\u8fc7\u4e2d\u95f4\u5916\u663e\u5b50\uff0c\u8fde\u63a5\u7b2c\u4e00\u6bb5\u4e0e\u7b2c\u4e09\u6bb5\uff0c\u5171\u56db\u6761\u3002\u7b2c\u4e09\u7c7b\u628a\u7b2c\u4e00\u5916\u663e\u5b50\u672b\u7aef\u5ef6\u957f\u4e94\u5341\u78b1\u57fa\uff0c\u518d\u63a5\u5230\u7b2c\u4e09\u6bb5\uff0c\u5171\u56db\u6761\u3002\u7b2c\u4e09\u7c7b\u4f7f\u7528\u4e86\u53c2\u8003\u6ce8\u91ca\u4e4b\u5916\u7684\u65b0\u4f9b\u4f53\u4f4d\u7f6e\uff0c\u4e0e\u7b2c\u4e8c\u7c7b\u7684\u5df2\u6709\u4f4d\u70b9\u65b0\u7ec4\u5408\u4e0d\u540c\u3002<\/p>\n

\u6240\u6709\u4e8c\u5341\u6761\u5e8f\u5217\u9996\u5148\u5b9e\u9645\u5b8c\u6210\u57fa\u56e0\u7ec4\u6bd4\u5bf9\u3002\u968f\u540e\uff0c\u6211\u4eec\u5728\u5176\u4e2d\u56db\u6761\u5df2\u77e5\u7ed3\u6784\u8bfb\u6bb5\u7684 BED \u8bb0\u5f55\u91cc\uff0c\u6545\u610f\u628a\u7b2c\u4e00\u5916\u663e\u5b50\u5757\u957f\u5ea6\u589e\u52a0\u4e09\u4e2a\u78b1\u57fa\uff0c\u6a21\u62df\u4e00\u4e2a\u53ef\u4ee5\u7cbe\u786e\u8ffd\u8e2a\u7684\u8fb9\u754c\u504f\u79fb\u3002\u539f\u59cb\u6bd4\u5bf9\u4e0e\u4fee\u6539\u8bb0\u5f55\u540c\u65f6\u4fdd\u5b58\uff0c\u56e0\u6b64\u8fd9\u4e9b\u504f\u79fb\u4e0d\u662f\u672c\u6b21\u6bd4\u5bf9\u5668\u81ea\u7136\u4ea7\u751f\u7684\u9519\u8bef\u3002<\/p>\n

\u8fd9\u79cd\u8bbe\u8ba1\u80fd\u591f\u6d4b\u8bd5\u6821\u6b63\u89c4\u5219\uff0c\u4f46\u4e0d\u80fd\u4f30\u8ba1\u771f\u5b9e\u957f\u8bfb\u957f\u5e73\u53f0\u7684\u9519\u8bef\u7387\u3002\u91cd\u590d\u751f\u6210\u7684\u76f8\u540c\u5e8f\u5217\u4e5f\u4e0d\u80fd\u5f53\u6210\u72ec\u7acb\u751f\u7269\u5b66\u91cd\u590d\u3002\u8bfb\u8005\u5e94\u628a\u5b83\u7406\u89e3\u6210\u4e00\u4e2a\u77e5\u9053\u8f93\u5165\u771f\u503c\u7684\u6d41\u7a0b\u68c0\u67e5\uff0c\u800c\u4e0d\u662f\u65b0\u7684\u526a\u63a5\u53d1\u73b0\u6216\u5de5\u5177\u51c6\u786e\u7387\u6392\u540d\u3002<\/p>\n

\u5b89\u88c5\u540e\u5148\u770b\u5f53\u524d\u6a21\u5757\u5e2e\u52a9<\/h2>\n

\u672c\u6b21\u5b89\u88c5\u7684\u5bb9\u5668\u5b9e\u9645\u62a5\u544a FLAIR \u4e09\u70b9\u96f6\u70b9\u4e00\uff0c\u590d\u73b0\u811a\u672c\u9501\u5b9a\u955c\u50cf\u6458\u8981\u3002\u8f6f\u4ef6\u5e2e\u52a9\u548c\u56fa\u5b9a\u7248\u672c\u6e90\u7801\u4e2d\uff0c\u8865\u5145\u8fde\u63a5\u8868\u53c2\u6570\u4e3a --junction_bed<\/code> \u6216 --junction_tab<\/code>\uff0c\u8fde\u63a5\u652f\u6301\u95e8\u69db\u4e3a --junction_support<\/code>\u3002\u8fd9\u4e9b\u5165\u53e3\u4e0e\u90e8\u5206\u65e7\u6559\u7a0b\u7684\u5199\u6cd5\u4e0d\u5b8c\u5168\u76f8\u540c\u3002<\/p>\n

docker pull quay.io\/biocontainers\/flair:3.0.1--pyhdfd78af_0\r\ndocker run --rm quay.io\/biocontainers\/flair:3.0.1--pyhdfd78af_0 \\\r\n  flair --version\r\n\r\n# \u89e3\u538b\u590d\u73b0\u5305\u540e\uff0c\u5728\u5bf9\u5e94\u5de5\u5177\u76ee\u5f55\u6267\u884c\r\npython3 run.py .\/practice\r\n<\/code><\/pre>\n
\u672c\u6b21\u6821\u6b63\u6a21\u5757\u4e0d\u63a5\u6536\u65e7\u793a\u4f8b\u4e2d\u5e38\u89c1\u7684\u57fa\u56e0\u7ec4\u53c2\u6570\uff0c\u800c\u6bd4\u5bf9\u548c\u5f52\u5e76\u6b65\u9aa4\u4ecd\u7136\u9700\u8981\u53c2\u8003\u5e8f\u5217\u3002\u9047\u5230\u53c2\u6570\u4e0d\u8bc6\u522b\u65f6\uff0c\u5e94\u5148\u6838\u5bf9\u5f53\u524d\u6a21\u5757\u5e2e\u52a9\uff0c\u4e0d\u8981\u628a\u53e6\u4e00\u4e2a\u6a21\u5757\u7684\u53c2\u6570\u76f4\u63a5\u642c\u8fc7\u6765\u3002\u672c\u6b21\u6838\u5bf9\u7684\u56fa\u5b9a\u7248\u672c\u6821\u6b63\u5165\u53e3<\/a><\/p>\n
\u540c\u4e00\u4e2a\u77ed\u9009\u9879\u5728\u4e0d\u540c\u6a21\u5757\u91cc\u4e5f\u53ef\u80fd\u542b\u4e49\u4e0d\u540c\u3002\u672c\u6b21\u7248\u672c\u4e2d\uff0c\u6821\u6b63\u6a21\u5757\u7684\u7a97\u53e3\u9009\u9879\u7528\u4e8e\u526a\u63a5\u4f4d\u70b9\u6821\u6b63\uff0c\u5f52\u5e76\u6a21\u5757\u5bf9\u5e94\u77ed\u9009\u9879\u5219\u7528\u4e8e\u8f6c\u5f55\u7aef\u70b9\u6bd4\u8f83\u3002\u8bb0\u5f55\u547d\u4ee4\u65f6\u5c3d\u91cf\u4fdd\u7559\u5b8c\u6574\u53c2\u6570\u540d\u548c\u6a21\u5757\u540d\uff0c\u80fd\u591f\u51cf\u5c11\u590d\u7528\u811a\u672c\u65f6\u7684\u8bef\u89e3\u3002<\/p>\n
\u4e24\u4e2a\u7a97\u53e3\u600e\u6837\u5904\u7406\u4e09\u4e2a\u78b1\u57fa\u7684\u504f\u79fb<\/h2>\n
\u4ec5\u63d0\u4f9b\u65e7\u6ce8\u91ca\u65f6\uff0c\u6211\u4eec\u5206\u522b\u8bbe\u7f6e\u4e24\u4e2a\u78b1\u57fa\u548c\u516d\u4e2a\u78b1\u57fa\u7684\u6821\u6b63\u7a97\u53e3\u3002\u5c0f\u7a97\u53e3\u7ec4\u4fdd\u7559\u5341\u4e8c\u6761\u3001\u6392\u9664\u516b\u6761\u3002\u88ab\u6392\u9664\u7684\u516b\u6761\u5305\u62ec\u56db\u6761\u4eba\u4e3a\u504f\u79fb\u8bb0\u5f55\uff0c\u4ee5\u53ca\u56db\u6761\u5177\u6709\u65b0\u4f9b\u4f53\u4f4d\u7f6e\u7684\u8bb0\u5f55\u3002<\/p>\n
\u7a97\u53e3\u6269\u5927\u5230\u516d\u540e\uff0c\u56db\u6761\u504f\u79fb\u8bb0\u5f55\u88ab\u6821\u6b63\u56de\u6ce8\u91ca\u8fb9\u754c\uff0c\u4fdd\u7559\u6570\u53d8\u6210\u5341\u516d\u3002\u68c0\u67e5\u8f93\u51fa\u53ef\u89c1\uff0c\u7b2c\u4e00\u5916\u663e\u5b50\u5757\u6062\u590d\u4e3a\u56db\u767e\u78b1\u57fa\uff0c\u540e\u9762\u7684\u5916\u663e\u5b50\u533a\u6bb5\u4fdd\u6301\u9884\u671f\u4f4d\u7f6e\u3002\u65b0\u4f9b\u4f53\u4f4d\u7f6e\u8ddd\u79bb\u65e7\u8fb9\u754c\u4e94\u5341\u78b1\u57fa\uff0c\u4ecd\u7136\u6ca1\u6709\u88ab\u8fd9\u6b21\u7a97\u53e3\u53d8\u5316\u63a5\u7eb3\u3002<\/p>\n
flair align -g genome.fa -r reads.fa -o aligned -t 2\r\n\r\n# perturbed.bed \u7531\u590d\u73b0\u811a\u672c\u5728\u5b9e\u9645\u6bd4\u5bf9\u540e\u751f\u6210\r\nflair correct -q perturbed.bed -f known.gtf \\\r\n  --ss_window 2 -t 2 -o window2\r\nflair correct -q perturbed.bed -f known.gtf \\\r\n  --ss_window 6 -t 2 -o window6\r\n<\/code><\/pre>\n
\u8fd9\u4e0d\u610f\u5473\u7740\u7a97\u53e3\u8d8a\u5927\u8d8a\u597d\u3002\u672c\u6b21\u77e5\u9053\u56db\u6761\u8bb0\u5f55\u88ab\u4eba\u4e3a\u79fb\u52a8\u4e86\u4e09\u4e2a\u78b1\u57fa\uff0c\u6240\u4ee5\u80fd\u591f\u5224\u65ad\u6062\u590d\u6b63\u786e\u3002\u771f\u5b9e\u6570\u636e\u4e2d\uff0c\u76f8\u90bb\u7684\u526a\u63a5\u4f4d\u70b9\u4e5f\u53ef\u80fd\u90fd\u5177\u6709\u751f\u7269\u5b66\u610f\u4e49\uff0c\u8fc7\u5927\u7684\u6821\u6b63\u8303\u56f4\u53ef\u80fd\u628a\u5b83\u4eec\u5408\u5728\u4e00\u8d77\u3002\u53c2\u6570\u7684\u5408\u7406\u6027\u9700\u8981\u7ed3\u5408\u8bef\u5dee\u5206\u5e03\u3001\u90bb\u8fd1\u4f4d\u70b9\u548c\u72ec\u7acb\u652f\u6301\u5224\u65ad\uff0c\u4e0d\u80fd\u53ea\u4ee5\u4fdd\u7559\u6761\u6570\u589e\u52a0\u4e3a\u76ee\u6807\u3002<\/p>\n
\u540c\u6837\uff0c\u4fdd\u7559\u6761\u6570\u51cf\u5c11\u4e5f\u4e0d\u81ea\u52a8\u4ee3\u8868\u5206\u6790\u66f4\u53ef\u9760\u3002\u82e5\u771f\u5b9e\u4f4d\u70b9\u672a\u8fdb\u5165\u53c2\u8003\u6216\u8005\u8865\u5145\u8bc1\u636e\uff0c\u5408\u7406\u8bfb\u6bb5\u4e5f\u53ef\u80fd\u88ab\u6392\u9664\u3002\u91cd\u8981\u7684\u662f\u6392\u9664\u7684\u5bf9\u8c61\u662f\u4ec0\u4e48\uff0c\u7f3a\u7684\u662f\u54ea\u4e00\u7c7b\u8bc1\u636e\uff0c\u800c\u4e0d\u662f\u628a\u67d0\u4e00\u4e2a\u6bd4\u4f8b\u5f53\u6210\u666e\u904d\u7684\u8d28\u91cf\u5206\u6570\u3002<\/p>\n
\u65b0\u4f4d\u70b9\u4e0e\u5df2\u77e5\u4f4d\u70b9\u7684\u65b0\u7ec4\u5408\u4e0d\u662f\u4e00\u56de\u4e8b<\/h2>\n
\u5728\u6ca1\u6709\u8865\u5145\u8fde\u63a5\u8868\u7684\u4e24\u7ec4\u4e2d\uff0c\u56db\u6761\u8df3\u8fc7\u4e2d\u95f4\u5916\u663e\u5b50\u7684\u8bfb\u6bb5\u90fd\u88ab\u4fdd\u7559\u3002\u8fd9\u4e9b\u8bfb\u6bb5\u8fde\u63a5\u4e86\u5df2\u77e5\u7b2c\u4e00\u5916\u663e\u5b50\u7684\u672b\u7aef\u548c\u5df2\u77e5\u7b2c\u4e09\u5916\u663e\u5b50\u7684\u8d77\u70b9\uff0c\u4e24\u4e2a\u8fb9\u754c\u672c\u8eab\u5df2\u7ecf\u80fd\u591f\u5728\u53c2\u8003\u4e2d\u627e\u5230\uff0c\u53d8\u5316\u7684\u662f\u5b83\u4eec\u7684\u7ec4\u5408\u3002<\/p>\n
\u53e6\u5916\u56db\u6761\u8bfb\u6bb5\u628a\u4f9b\u4f53\u4f4d\u7f6e\u5411\u540e\u79fb\u52a8\u4e86\u4e94\u5341\u78b1\u57fa\uff0c\u8fd9\u4e2a\u65b0\u8fb9\u754c\u4e0d\u5728\u539f\u53c2\u8003\u4e2d\uff0c\u56e0\u6b64\u4ecd\u7136\u88ab\u6392\u9664\u3002\u4e24\u79cd\u60c5\u51b5\u90fd\u53ef\u4ee5\u4ea7\u751f\u53c2\u8003\u4e4b\u5916\u7684\u8f6c\u5f55\u7ed3\u6784\uff0c\u5374\u4e0d\u5177\u6709\u76f8\u540c\u7684\u4f4d\u70b9\u8bc1\u636e\u6761\u4ef6\u3002<\/p>\n
\u8fd9\u4e2a\u533a\u522b\u80fd\u591f\u5e2e\u52a9\u7406\u89e3\u5de5\u5177\u5173\u4e8e\u65b0\u4f4d\u70b9\u652f\u6301\u7684\u8981\u6c42\u3002\u4e0d\u80fd\u628a\u201c\u672a\u63d0\u4f9b\u989d\u5916\u4f4d\u70b9\u8bc1\u636e\u201d\u7b80\u5355\u7b49\u540c\u4e8e\u201c\u7edd\u4e0d\u53ef\u80fd\u8f93\u51fa\u4efb\u4f55\u53c2\u8003\u4e4b\u5916\u7684\u8f6c\u5f55\u672c\u201d\u3002\u9605\u8bfb\u5019\u9009\u65f6\u5e94\u5206\u522b\u6807\u6ce8\u5df2\u6709\u4f4d\u70b9\u7684\u65b0\u8fde\u63a5\u3001\u65b0\u4f9b\u4f53\u6216\u53d7\u4f53\u4f4d\u7f6e\u3001\u7aef\u70b9\u53d8\u5316\u7b49\u60c5\u51b5\uff0c\u518d\u51b3\u5b9a\u9700\u8981\u600e\u6837\u7684\u9a8c\u8bc1\u3002<\/p>\n
\u8865\u5145\u8868\u4e2d\u7684\u5206\u6570\u4e5f\u662f\u8fc7\u6ee4\u6761\u4ef6<\/h2>\n
\u4e3a\u4e86\u5355\u72ec\u6d4b\u8bd5\u8fde\u63a5\u8868\u7684\u5904\u7406\uff0c\u6211\u4eec\u4eba\u4e3a\u5199\u5165\u4e00\u4e2a\u65b0\u4f9b\u4f53\u8fde\u63a5\uff0c\u5206\u6570\u5b57\u6bb5\u56fa\u5b9a\u4e3a\u5341\u3002\u8fd9\u5f20\u8868\u662f\u89c4\u5219\u6d4b\u8bd5\u8f93\u5165\uff0c\u6ca1\u6709\u6765\u81ea\u771f\u5b9e\u77ed\u8bfb\u957f\u6d4b\u5e8f\u7684\u8bc1\u636e\uff0c\u4e0d\u80fd\u79f0\u4e3a\u72ec\u7acb\u5b9e\u9a8c\u9a8c\u8bc1\u3002\u771f\u5b9e\u9879\u76ee\u5e94\u4f7f\u7528\u53ef\u9760\u7684\u6d4b\u5e8f\u8fde\u63a5\u8bc1\u636e\u751f\u6210\u76f8\u5e94\u6587\u4ef6\u3002<\/p>\n
\u5728\u8fd9\u4efd\u8868\u4e0a\uff0c\u5c06\u8fde\u63a5\u652f\u6301\u95e8\u69db\u8bbe\u4e3a\u5341\u65f6\uff0c\u65b0\u4f4d\u70b9\u4fe1\u606f\u88ab\u63a5\u53d7\uff0c\u5168\u90e8\u4e8c\u5341\u6761\u8bb0\u5f55\u901a\u8fc7\u6821\u6b63\u3002\u628a\u95e8\u69db\u6539\u4e3a\u5341\u4e00\uff0c\u8868\u4e2d\u8fd9\u6761\u8fde\u63a5\u4e0d\u518d\u6ee1\u8db3\u6761\u4ef6\uff0c\u4fdd\u7559\u6570\u56de\u5230\u5341\u516d\uff0c\u65b0\u4f9b\u4f53\u76f8\u5173\u56db\u6761\u518d\u6b21\u88ab\u6392\u9664\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
\u6821\u6b63\u6761\u4ef6<\/th>\n\u4fdd\u7559<\/th>\n\u6392\u9664<\/th>\n<\/tr>\n<\/thead>\n
\u7a97\u53e3\u4e3a 2<\/td>\n12<\/td>\n8<\/td>\n<\/tr>\n
\u7a97\u53e3\u4e3a 6<\/td>\n16<\/td>\n4<\/td>\n<\/tr>\n
\u8868\u95e8\u69db 10<\/td>\n20<\/td>\n0<\/td>\n<\/tr>\n
\u8868\u95e8\u69db 11<\/td>\n16<\/td>\n4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
# \u4ec5\u4e3a\u89c4\u5219\u7ec3\u4e60\u6784\u9020\u8868\uff0c\u5206\u6570 10 \u4e0d\u662f\u5b9e\u6d4b\u77ed\u8bfb\u6bb5\u652f\u6301\u6570\r\nprintf 'chrToy\\t1450\\t4000\\tfixture_new_donor\\t10\\t+\\n' \\\r\n  > designed_junctions.bed\r\n\r\nflair correct -q perturbed.bed -f known.gtf \\\r\n  --ss_window 6 --junction_bed designed_junctions.bed \\\r\n  --junction_support 10 -t 2 -o junction10\r\n<\/code><\/pre>\n
\u8be5\u8868\u91c7\u7528\u96f6\u57fa\u5236\u534a\u5f00\u533a\u95f4\u3002\u8d77\u70b9\u4e00\u5343\u56db\u767e\u4e94\u5341\uff0c\u5bf9\u5e94\u524d\u9762\u5916\u663e\u5b50\u7684\u4e00\u57fa\u5236\u672b\u7aef\u4e00\u5343\u56db\u767e\u4e94\u5341\uff0c\u800c\u5185\u542b\u5b50\u7b2c\u4e00\u4e2a\u78b1\u57fa\u4e3a\u4e00\u5343\u56db\u767e\u4e94\u5341\u4e00\u3002\u5750\u6807\u4f53\u7cfb\u6df7\u7528\u65f6\uff0c\u54ea\u6015\u53ea\u5dee\u4e00\u4e2a\u78b1\u57fa\uff0c\u4e5f\u53ef\u80fd\u4f7f\u672c\u6765\u6e05\u695a\u7684\u4f4d\u70b9\u5173\u7cfb\u53d8\u5f97\u5f02\u5e38\u3002\u751f\u6210\u8f93\u5165\u524d\uff0c\u5e94\u5bf9\u7167\u4e00\u4e2a\u5df2\u77e5\u8fde\u63a5\u624b\u7b97\u8f6c\u6362\u662f\u5426\u6b63\u786e\u3002<\/p>\n
\u8fd8\u5e94\u533a\u5206\u8fde\u63a5\u8868\u7684\u5206\u6570\u3001\u6bd4\u5bf9\u8d28\u91cf\u548c\u6700\u7ec8\u5f02\u6784\u4f53\u652f\u6301\u6570\u3002\u5b83\u4eec\u5904\u5728\u4e0d\u540c\u9636\u6bb5\uff0c\u4ee3\u8868\u4e0d\u540c\u8bc1\u636e\u3002\u672c\u6b21\u8868\u95e8\u69db\u5341\u4e0e\u540e\u9762\u7684\u6a21\u578b\u95e8\u69db\u4e09\u6216\u4e94\uff0c\u5e76\u4e0d\u662f\u540c\u4e00\u79cd\u91cf\uff0c\u4e0d\u80fd\u4e3a\u4e86\u8ba9\u7ed3\u679c\u901a\u8fc7\u800c\u76f8\u4e92\u66ff\u6362\u3002<\/p>\n
\u4e8c\u5341\u6761\u901a\u8fc7\u6821\u6b63\u540e\uff0c\u53ea\u7559\u4e0b\u4e00\u4e2a\u6a21\u578b\u4e5f\u53ef\u80fd\u6b63\u5e38<\/h2>\n
\u6211\u4eec\u628a\u4fdd\u7559\u4e8c\u5341\u6761\u7684\u6821\u6b63\u7ed3\u679c\u9001\u5165\u5f52\u5e76\u6b65\u9aa4\uff0c\u542f\u7528\u5b8c\u6574\u8986\u76d6\u548c\u526a\u63a5\u68c0\u67e5\uff0c\u5e76\u751f\u6210\u8bfb\u6bb5\u5230\u6a21\u578b\u7684\u5bf9\u5e94\u6587\u4ef6\u3002\u7b2c\u4e00\u7ec4\u8981\u6c42\u81f3\u5c11\u4e09\u6761\u652f\u6301\uff0c\u7b2c\u4e8c\u7ec4\u8981\u6c42\u81f3\u5c11\u4e94\u6761\uff0c\u5176\u4ed6\u8bbe\u7f6e\u4fdd\u6301\u4e00\u81f4\u3002<\/p>\n
\u95e8\u69db\u4e3a\u4e09\u65f6\uff0c\u6700\u7ec8\u4fdd\u7559\u5df2\u77e5\u4e09\u5916\u663e\u5b50\u6a21\u578b\u3001\u8df3\u8dc3\u6a21\u578b\u548c\u65b0\u4f9b\u4f53\u6a21\u578b\uff0c\u5171\u4e09\u4e2a\u3002\u5bf9\u5e94\u6587\u4ef6\u4e2d\uff0c\u5b83\u4eec\u5206\u522b\u6709\u5341\u4e8c\u3001\u56db\u3001\u56db\u6761\u8bfb\u6bb5\u3002\u95e8\u69db\u4e3a\u4e94\u65f6\uff0c\u53ea\u5269\u5177\u6709\u5341\u4e8c\u6761\u652f\u6301\u7684\u5df2\u77e5\u6a21\u578b\uff0c\u53e6\u5916\u4e24\u4e2a\u6a21\u578b\u4e0d\u518d\u8fdb\u5165\u6700\u7ec8\u96c6\u5408\u3002<\/p>\n
flair collapse -g genome.fa \\\r\n  -q junction10_all_corrected.bed -r reads.fa -f known.gtf \\\r\n  -t 2 -o support3 --support 3 \\\r\n  --stringent --check_splice --generate_map \\\r\n  --no_redundant longest --no_gtf_end_adjustment\r\n<\/code><\/pre>\n
\n\n\n\n\n\n\n
\u6a21\u578b\u95e8\u69db<\/th>\n\u6700\u7ec8\u6a21\u578b\u6570<\/th>\n\u6700\u7ec8\u6620\u5c04\u8bfb\u6bb5\u6570<\/th>\n<\/tr>\n<\/thead>\n
3<\/td>\n3<\/td>\n20<\/td>\n<\/tr>\n
5<\/td>\n1<\/td>\n12<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8fd9\u8bf4\u660e\u901a\u8fc7\u6821\u6b63\u53ea\u662f\u8fdb\u5165\u4e0b\u4e00\u9636\u6bb5\u7684\u6761\u4ef6\u4e4b\u4e00\u3002\u6700\u7ec8\u96c6\u5408\u53d7\u5230\u6a21\u578b\u652f\u6301\u95e8\u69db\u5f71\u54cd\uff0c\u6ca1\u6709\u62a5\u544a\u7684\u6a21\u578b\u4ecd\u7136\u53ef\u80fd\u5728\u8f93\u5165\u4e2d\u6709\u5c11\u91cf\u8bfb\u6bb5\u3002\u8fd9\u91cc\u7684\u652f\u6301\u6570\u4e5f\u4e0d\u80fd\u66ff\u4ee3\u751f\u7269\u5b66\u91cd\u590d\uff0c\u66f4\u4e0d\u80fd\u76f4\u63a5\u5f62\u6210\u5dee\u5f02\u526a\u63a5\u7684\u7edf\u8ba1\u7ed3\u8bba\u3002<\/p>\n
\u672c\u6b21\u8fd8\u660e\u786e\u5173\u95ed\u53c2\u8003\u7aef\u70b9\u8c03\u6574\uff0c\u5e76\u4e3a\u540c\u4e00\u526a\u63a5\u94fe\u9009\u62e9\u6700\u957f\u7aef\u70b9\u7ec4\u5408\uff0c\u76ee\u7684\u662f\u8ba9\u4eba\u5de5\u8bbe\u8ba1\u7684\u9996\u5c3e\u4f4d\u7f6e\u5bb9\u6613\u6838\u5bf9\u3002\u771f\u5b9e\u6570\u636e\u4e2d\u7684\u622a\u77ed\u3001\u964d\u89e3\u548c\u7aef\u70b9\u5f02\u8d28\u6027\u9700\u8981\u53e6\u884c\u8bc4\u4f30\uff0c\u4e0d\u5e94\u628a\u8fd9\u4e9b\u9009\u9879\u5f53\u6210\u6240\u6709\u5b9e\u9a8c\u901a\u7528\u7684\u6700\u4f18\u7ec4\u5408\u3002<\/p>\n
\u8ba1\u6570\u6587\u4ef6\u8fd8\u5217\u7740\u4e09\u6761\uff0c\u4e3a\u4f55\u6700\u7ec8\u53ea\u6709\u4e00\u6761<\/h2>\n
\u5728\u95e8\u69db\u4e3a\u4e94\u7684\u8fd0\u884c\u4e2d\uff0c\u8fd8\u6709\u4e00\u4e2a\u5bb9\u6613\u8bef\u8bfb\u7684\u7ec6\u8282\u3002\u4ea7\u751f\u7684\u5f02\u6784\u4f53\u8ba1\u6570\u6587\u4ef6\u4ecd\u7136\u5217\u51fa\u5341\u4e8c\u3001\u56db\u3001\u56db\u8fd9\u4e09\u7ec4\u5019\u9009\u652f\u6301\uff0c\u4f46\u6700\u7ec8 BED\u3001\u5e8f\u5217\u6587\u4ef6\u548c\u8bfb\u6bb5\u5bf9\u5e94\u6587\u4ef6\u53ea\u4fdd\u7559\u5df2\u77e5\u6a21\u578b\u3002<\/p>\n
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