{"id":73129,"date":"2026-11-15T13:59:41","date_gmt":"2026-11-15T05:59:41","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73129"},"modified":"2026-11-15T13:59:41","modified_gmt":"2026-11-15T05:59:41","slug":"sqanti3-fsm-nic-nnc-reference-coding-na-interpretation-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/sqanti3-fsm-nic-nnc-reference-coding-na-interpretation-practice\/","title":{"rendered":"SQANTI3 \u5b9e\u8dd1\uff0cFSM\u3001NIC\u3001NNC \u80fd\u544a\u8bc9\u4f60\u4ec0\u4e48\uff0c\u53c8\u4e0d\u80fd\u8bc1\u660e\u4ec0\u4e48"},"content":{"rendered":"
\n

\u957f\u8bfb\u957f\u8f6c\u5f55\u7ec4\u7ed3\u679c\u91cc\uff0c\u7ecf\u5e38\u51fa\u73b0\u5b8c\u6574\u526a\u63a5\u5339\u914d\u3001\u5df2\u77e5\u4f4d\u70b9\u65b0\u7ec4\u5408\u4ee5\u53ca\u5305\u542b\u65b0\u4f4d\u70b9\u7b49\u5206\u7c7b\u3002\u5b83\u4eec\u770b\u8d77\u6765\u50cf\u4e00\u5957\u4ece\u53ef\u9760\u5230\u53ef\u7591\u7684\u8d28\u91cf\u7b49\u7ea7\uff0c\u4f46\u5b9e\u9645\u56de\u7b54\u7684\u9996\u5148\u662f\u8f93\u5165\u7ed3\u6784\u4e0e\u5f53\u524d\u53c2\u8003\u6ce8\u91ca\u7684\u5173\u7cfb\u3002\u5982\u679c\u628a\u5206\u7c7b\u6807\u7b7e\u76f4\u63a5\u7b49\u540c\u4e8e\u771f\u5047\uff0c\u5c31\u5bb9\u6613\u628a\u91cd\u8981\u5019\u9009\u8bef\u5220\uff0c\u4e5f\u53ef\u80fd\u7ed9\u7f3a\u5c11\u9a8c\u8bc1\u7684\u7ed3\u679c\u8fc7\u5ea6\u80cc\u4e66\u3002<\/p>\n

\u672c\u7bc7\u5b9e\u9645\u5b89\u88c5 SQANTI3 \u516d\u70b9\u4e00\uff0c\u6784\u9020\u516b\u79cd\u53ef\u4ee5\u624b\u5de5\u6838\u5bf9\u7684\u8f6c\u5f55\u672c\u7ed3\u6784\uff0c\u5206\u522b\u5bf9\u7167\u4e24\u5957\u53c2\u8003\u6ce8\u91ca\u8fd0\u884c\u8d28\u91cf\u6ce8\u91ca\u3002\u6211\u4eec\u91cd\u70b9\u68c0\u67e5\u526a\u63a5\u7c7b\u522b\u3001\u7aef\u70b9\u5dee\u5f02\u3001\u5178\u578b\u8fde\u63a5\u548c\u7f3a\u5931\u8bc1\u636e\uff0c\u540c\u65f6\u8bb0\u5f55\u5b89\u88c5\u4e0e\u6267\u884c\u4e2d\u9047\u5230\u7684\u95ee\u9898\uff0c\u8ba9\u8bfb\u8005\u80fd\u591f\u7406\u89e3\u6bcf\u4e00\u5217\u5230\u5e95\u6765\u81ea\u4ec0\u4e48\u5206\u6790\u3002<\/p>\n

\"\u7ed3\u6784\u7c7b\u522b\uff0c\u4e0d\u80fd\u76f4\u63a5\u5f53\u4f5c\u771f\u5047\u3002\u516b\u79cd\u8bbe\u8ba1\u7ed3\u6784\uff0c\u4e24\u5957\u53c2\u8003\uff0c\u9010\u5217\u68c0\u67e5\u8bc1\u636e\u3002NIC
\u516b\u79cd\u8bbe\u8ba1\u7ed3\u6784\uff0c\u4e24\u5957\u53c2\u8003\uff0c\u9010\u5217\u68c0\u67e5\u8bc1\u636e<\/figcaption><\/figure>\n

\u672c\u6b21\u68c0\u67e5\u7684\u662f\u7ed3\u6784\uff0c\u4e0d\u662f\u8868\u8fbe\u53d1\u73b0<\/h2>\n

\u8f93\u5165\u7531\u4eba\u5de5\u57fa\u56e0\u7ec4\u548c\u624b\u5de5\u8bbe\u8ba1\u7684 GTF \u6784\u6210\uff0c\u6ca1\u6709\u771f\u5b9e\u6d4b\u5e8f\u6837\u672c\u3002\u53c2\u8003\u6ce8\u91ca\u9996\u5148\u53ea\u5305\u542b\u4e00\u4e2a\u4e09\u5916\u663e\u5b50\u8f6c\u5f55\u672c\uff0c\u7b2c\u4e8c\u5957\u53c2\u8003\u518d\u589e\u52a0\u4e00\u4e2a\u8df3\u8fc7\u4e2d\u95f4\u5916\u663e\u5b50\u7684\u6a21\u578b\u3002\u4e24\u6b21\u8fd0\u884c\u7684\u516b\u6761\u67e5\u8be2\u7ed3\u6784\u5b8c\u5168\u76f8\u540c\u3002<\/p>\n

\u6211\u4eec\u6ca1\u6709\u63d0\u4f9b\u77ed\u8bfb\u6bb5\u8986\u76d6\u3001\u8868\u8fbe\u77e9\u9635\u3001CAGE \u5cf0\u3001\u72ec\u7acb\u591a\u805a\u817a\u82f7\u4f4d\u70b9\u6216\u771f\u5b9e\u5168\u957f\u8bfb\u6bb5\u8ba1\u6570\uff0c\u4e5f\u6ca1\u6709\u8fd0\u884c ORF \u9884\u6d4b\u3001\u89c4\u5219\u8fc7\u6ee4\u548c\u5019\u9009\u6551\u56de\u3002\u56e0\u6b64\uff0c\u672c\u6587\u7ed3\u679c\u80fd\u591f\u68c0\u9a8c\u7ed3\u6784\u5206\u7c7b\u4e0e\u5b57\u6bb5\u884c\u4e3a\uff0c\u4e0d\u80fd\u8bc1\u660e\u65b0\u7684\u751f\u7269\u5b66\u8f6c\u5f55\u672c\uff0c\u4e5f\u4e0d\u80fd\u8bc4\u4f30\u6574\u5957\u53d1\u73b0\u6d41\u7a0b\u7684\u51c6\u786e\u7387\u3002<\/p>\n

\u8f93\u5165\u76f4\u63a5\u91c7\u7528 GTF\uff0c\u6240\u4ee5\u6ca1\u6709\u6267\u884c\u5e8f\u5217\u5230\u57fa\u56e0\u7ec4\u7684\u6bd4\u5bf9\u3002\u65e5\u5fd7\u8fd8\u660e\u786e\u8bf4\u660e\uff0c\u8fd9\u79cd\u5165\u53e3\u6ca1\u6709\u8ba1\u7b97\u6bd4\u5bf9\u63d2\u5165\u7f3a\u5931\u4fe1\u606f\u3002\u540e\u7eed\u770b\u5230\u76f8\u5173\u5b57\u6bb5\u4e3a\u7a7a\uff0c\u5e94\u5148\u8054\u7cfb\u5b9e\u9645\u8f93\u5165\u65b9\u5f0f\uff0c\u800c\u4e0d\u662f\u9a6c\u4e0a\u63a8\u65ad\u8be5\u8f6c\u5f55\u672c\u6ca1\u6709\u4efb\u4f55\u6bd4\u5bf9\u9519\u8bef\u3002<\/p>\n

SQANTI3 \u53ef\u4ee5\u6574\u5408\u591a\u79cd\u72ec\u7acb\u8bc1\u636e\uff0c\u4f46\u8fd9\u79cd\u80fd\u529b\u53ea\u6709\u5728\u76f8\u5e94\u6570\u636e\u548c\u5206\u6790\u6b65\u9aa4\u5b9e\u9645\u5b58\u5728\u65f6\u624d\u4f1a\u4f53\u73b0\u5728\u7ed3\u679c\u4e2d\u3002\u914d\u7f6e\u6587\u4ef6\u91cc\u6709\u4e00\u4e2a\u53ef\u9009\u5165\u53e3\uff0c\u4e0d\u7b49\u4e8e\u5f53\u524d\u9879\u76ee\u5df2\u7ecf\u83b7\u5f97\u4e86\u90a3\u4e00\u7c7b\u652f\u6301\u3002SQANTI3 \u8d28\u91cf\u68c0\u67e5\u8bf4\u660e<\/a><\/p>\n

\u516b\u79cd\u7ed3\u6784\u5206\u522b\u63a7\u5236\u4ec0\u4e48\u53d8\u91cf<\/h2>\n

\u53c2\u8003\u7684\u4e09\u4e2a\u5916\u663e\u5b50\u5206\u522b\u4f4d\u4e8e\u4e00\u5343\u96f6\u4e00\u5230\u4e00\u5343\u56db\u767e\u3001\u4e8c\u5343\u4e94\u767e\u96f6\u4e00\u5230\u4e8c\u5343\u4e5d\u767e\u3001\u56db\u5343\u96f6\u4e00\u5230\u56db\u5343\u56db\u767e\u3002\u6240\u6709\u5750\u6807\u6784\u9020\u548c\u968f\u673a\u79cd\u5b50\u90fd\u4fdd\u5b58\u5728\u811a\u672c\u91cc\uff0c\u65b9\u4fbf\u68c0\u67e5\u6bcf\u4e00\u79cd\u53d8\u5316\u3002<\/p>\n

\u7b2c\u4e00\u6761\u67e5\u8be2\u4fdd\u7559\u5168\u90e8\u5185\u90e8\u8fde\u63a5\uff0c\u4f46\u8d77\u70b9\u5411\u540e\u79fb\u52a8\u4e8c\u5341\u78b1\u57fa\uff0c\u7ec8\u70b9\u5411\u524d\u79fb\u52a8\u4e8c\u5341\u78b1\u57fa\u3002\u7b2c\u4e8c\u6761\u5220\u9664\u6700\u524d\u9762\u7684\u5916\u663e\u5b50\u3002\u7b2c\u4e09\u6761\u8df3\u8fc7\u4e2d\u95f4\u5916\u663e\u5b50\uff0c\u53ea\u8fde\u63a5\u7b2c\u4e00\u6bb5\u548c\u7b2c\u4e09\u6bb5\u3002<\/p>\n

\u7b2c\u56db\u6761\u628a\u7b2c\u4e00\u5916\u663e\u5b50\u7684\u4f9b\u4f53\u5ef6\u957f\u4e94\u5341\u78b1\u57fa\uff0c\u5e76\u5728\u65b0\u4f4d\u7f6e\u8bbe\u7f6e\u5178\u578b\u8fde\u63a5\u5e8f\u5217\u3002\u7b2c\u4e94\u6761\u518d\u4f7f\u7528\u53e6\u4e00\u4e2a\u65b0\u4f9b\u4f53\u4f4d\u7f6e\uff0c\u6545\u610f\u8bbe\u7f6e\u975e\u5178\u578b\u8fde\u63a5\u3002\u6700\u540e\u4e09\u6761\u5206\u522b\u4f4d\u4e8e\u53c2\u8003\u7684\u53cd\u4e49\u94fe\u3001\u4e00\u4e2a\u5185\u542b\u5b50\u5185\u90e8\u548c\u57fa\u56e0\u95f4\u533a\u3002<\/p>\n

\u57fa\u56e0\u95f4\u533a\u67e5\u8be2\u7684\u7ec8\u70b9\u4e0b\u6e38\u4e8c\u5341\u78b1\u57fa\u88ab\u5168\u90e8\u8bbe\u4e3a\u817a\u560c\u5464\u3002\u8fd9\u662f\u7528\u4e8e\u68c0\u9a8c\u5b57\u6bb5\u5c3a\u5ea6\u7684\u4eba\u5de5\u6781\u7aef\u6761\u4ef6\uff0c\u4e0d\u662f\u6211\u4eec\u5728\u771f\u5b9e\u6837\u672c\u91cc\u53d1\u73b0\u4e86\u5185\u90e8\u5f15\u7269\u8d77\u59cb\u4e8b\u4ef6\u3002\u533a\u5206\u6d4b\u8bd5\u6784\u9020\u548c\u751f\u7269\u5b66\u89c2\u5bdf\uff0c\u80fd\u907f\u514d\u5b9e\u9a8c\u7ed3\u8bba\u88ab\u8bef\u8bfb\u3002<\/p>\n

\u5b89\u88c5\u6210\u529f\u540e\u4ecd\u8981\u6267\u884c\u4e00\u4e2a\u5c0f\u68c0\u67e5<\/h2>\n

\u672c\u6b21\u5bb9\u5668\u5185\u8d28\u91cf\u68c0\u67e5\u7a0b\u5e8f\u5b9e\u9645\u62a5\u544a\u516d\u70b9\u4e00\u3002\u7edf\u4e00\u5305\u88c5\u5165\u53e3\u4e0d\u63a5\u53d7\u6211\u4eec\u6700\u521d\u5c1d\u8bd5\u7684\u7248\u672c\u53c2\u6570\uff0c\u8d28\u91cf\u68c0\u67e5\u5165\u53e3\u53ef\u4ee5\u6b63\u5e38\u62a5\u544a\u7248\u672c\u3002\u5148\u770b\u5177\u4f53\u5b50\u7a0b\u5e8f\u5e2e\u52a9\uff0c\u6bd4\u53ea\u8bb0\u4f4f\u8f6f\u4ef6\u603b\u540d\u79f0\u66f4\u53ef\u9760\u3002<\/p>\n

IMAGE=quay.io\/biocontainers\/sqanti3@sha256:998119e65224de4cbaa8ef4a43e42e88705d8c76436509ec031b3a9bcac2581f\r\ndocker pull "$IMAGE"\r\ndocker run --rm "$IMAGE" sqanti3_qc.py --version\r\ndocker run --rm "$IMAGE" sqanti3_qc.py --help\r\n<\/code><\/pre>\n
\u7b2c\u4e00\u8f6e\u6d41\u7a0b\u5728\u6ce8\u91ca\u683c\u5f0f\u8f6c\u6362\u5904\u5931\u8d25\u3002\u5177\u4f53\u65e5\u5fd7\u663e\u793a\uff0c\u5185\u7f6e\u8f6c\u6362\u7a0b\u5e8f\u8bf7\u6c42\u7684\u5e93\u540d\u79f0\u4e0d\u5b58\u5728\uff0c\u800c\u955c\u50cf\u91cc\u5df2\u7ecf\u5305\u542b\u540c\u7cfb\u5217\u7684\u5b9e\u9645\u5e93\u6587\u4ef6\u3002\u6211\u4eec\u6ca1\u6709\u4fee\u6539\u57fa\u7840\u955c\u50cf\uff0c\u800c\u662f\u5728\u5b9e\u9a8c\u76ee\u5f55\u4e2d\u7ed9\u5df2\u6709\u5e93\u589e\u52a0\u6240\u9700\u522b\u540d\uff0c\u53ea\u5bf9\u672c\u6b21\u5bb9\u5668\u8c03\u7528\u8bbe\u7f6e\u5e93\u641c\u7d22\u8def\u5f84\u3002<\/p>\n
gtfToGenePred-linux-x86_64\r\nerror while loading shared libraries:\r\nlibbz2.so.1: cannot open shared object file\r\n<\/code><\/pre>\n
\u4fee\u590d\u540e\uff0c\u53e6\u4e00\u4e2a\u53ef\u9009\u7684\u5339\u914d\u660e\u7ec6\u8f93\u51fa\u89e6\u53d1\u51fd\u6570\u53c2\u6570\u6570\u91cf\u9519\u8bef\u3002\u7531\u4e8e\u5b83\u4e0d\u5c5e\u4e8e\u672c\u5b9e\u9a8c\u6240\u9700\u7684\u5206\u7c7b\u8868\u548c\u8fde\u63a5\u8868\uff0c\u6211\u4eec\u53bb\u6389\u8be5\u53ef\u9009\u5f00\u5173\uff0c\u5728\u5168\u65b0\u7ed3\u679c\u76ee\u5f55\u91cd\u65b0\u6267\u884c\u4e24\u7ec4\u5206\u6790\uff0c\u4e24\u7ec4\u5747\u6b63\u5e38\u9000\u51fa\u3002<\/p>\n
\u53ef\u9009\u53c2\u6570 --isoform_hits\r\nTypeError: write_isoform_hits() takes 2 positional arguments but 3 were given\r\n<\/code><\/pre>\n
\u9644\u4ef6\u9a71\u52a8\u5305\u542b\u8fd9\u6b21\u56fa\u5b9a\u955c\u50cf\u7684\u5c40\u90e8\u5e93\u517c\u5bb9\u5904\u7406\uff0c\u5e76\u4fdd\u7559\u539f\u59cb\u548c\u4fee\u590d\u540e\u7684\u4f9d\u8d56\u68c0\u67e5\u7ed3\u679c\u3002\u8fd9\u4e2a\u7ecf\u9a8c\u4e0d\u80fd\u63a8\u5e7f\u4e3a\u9047\u5230\u5e93\u9519\u8bef\u5c31\u968f\u610f\u5efa\u7acb\u8f6f\u94fe\u63a5\uff0c\u5e94\u8be5\u5148\u6838\u5bf9\u7f3a\u5931\u540d\u79f0\u3001\u5b9e\u9645\u5e93\u7248\u672c\u4e0e\u8fd0\u884c\u7ed3\u679c\u3002\u66f4\u6362\u955c\u50cf\u540e\uff0c\u4e5f\u8981\u91cd\u65b0\u786e\u8ba4\u95ee\u9898\u662f\u5426\u4ecd\u7136\u5b58\u5728\u3002<\/p>\n
\u6700\u7ec8\u5b9e\u9645\u6267\u884c\u7684\u547d\u4ee4<\/h2>\n
\u4e0b\u9762\u662f\u5c40\u90e8\u517c\u5bb9\u73af\u5883\u51c6\u5907\u5b8c\u6210\u540e\uff0c\u5bb9\u5668\u5185\u6267\u884c\u7684\u6838\u5fc3\u547d\u4ee4\u3002\u7b2c\u4e8c\u7ec4\u53ea\u628a\u53c2\u8003\u6587\u4ef6\u6362\u6210\u589e\u52a0\u8df3\u8dc3\u6a21\u578b\u7684\u7248\u672c\uff0c\u5e76\u4f7f\u7528\u53e6\u4e00\u4e2a\u8f93\u51fa\u76ee\u5f55\u3002\u5b8c\u6574\u9a71\u52a8\u4f1a\u81ea\u52a8\u751f\u6210\u5168\u90e8\u8f93\u5165\uff0c\u4e0d\u8981\u6c42\u8bfb\u8005\u624b\u5de5\u62fc\u63a5 GTF\u3002<\/p>\n
sqanti3_qc.py --isoforms queries.gtf \\\r\n  --refGTF reference.gtf --refFasta genome.fa \\\r\n  --cpus 2 --report skip \\\r\n  --output toy --dir baseline\r\n\r\nsqanti3_qc.py --isoforms queries.gtf \\\r\n  --refGTF expanded.gtf --refFasta genome.fa \\\r\n  --cpus 2 --report skip \\\r\n  --output toy --dir expanded\r\n<\/code><\/pre>\n
\u5f53\u524d\u7248\u672c\u9700\u8981\u663e\u5f0f\u5f00\u542f ORF \u9884\u6d4b\uff0c\u672c\u6b21\u6ca1\u6709\u5f00\u542f\u3002\u7f51\u9875\u62a5\u544a\u4e5f\u88ab\u660e\u786e\u8df3\u8fc7\uff0c\u4f46\u5206\u7c7b\u8868\u548c\u8fde\u63a5\u8868\u90fd\u5df2\u751f\u6210\u3002\u62a5\u544a\u662f\u5426\u6f02\u4eae\u4e0e\u5e95\u5c42\u8868\u683c\u662f\u5426\u6b63\u786e\u662f\u4e24\u4ef6\u4e8b\uff0c\u590d\u73b0\u65f6\u5e94\u5148\u6838\u5bf9\u6570\u636e\u6587\u4ef6\u3002<\/p>\n
python3 151-sqanti3\/run.py results\/sqanti3\r\n<\/code><\/pre>\n
FSM \u5e76\u4e0d\u8981\u6c42\u4e24\u4e2a\u7aef\u70b9\u5b8c\u5168\u76f8\u540c<\/h2>\n
\u7b2c\u4e00\u6761\u67e5\u8be2\u6bd4\u53c2\u8003\u4e24\u7aef\u5404\u77ed\u4e8c\u5341\u78b1\u57fa\uff0c\u4f46\u5185\u90e8\u526a\u63a5\u8fde\u63a5\u5b8c\u5168\u4e00\u81f4\uff0c\u5b9e\u9645\u5206\u7c7b\u4ecd\u662f\u5b8c\u6574\u526a\u63a5\u5339\u914d\u3002\u7ed3\u679c\u4e2d\u7684\u4e24\u9879\u7aef\u70b9\u5dee\u5f02\u90fd\u4e3a\u8d1f\u4e8c\u5341\uff0c\u800c\u67e5\u8be2\u957f\u5ea6\u662f\u4e00\u5343\u4e00\u767e\u516d\u5341\uff0c\u53c2\u8003\u957f\u5ea6\u662f\u4e00\u5343\u4e8c\u767e\u3002<\/p>\n
\u56e0\u6b64\uff0c\u5b8c\u6574\u526a\u63a5\u5339\u914d\u63cf\u8ff0\u7684\u662f\u526a\u63a5\u7ed3\u6784\u7684\u5339\u914d\u5173\u7cfb\uff0c\u4e0d\u80fd\u81ea\u52a8\u89e3\u91ca\u6210\u6574\u6761\u8f6c\u5f55\u672c\u4ece\u8d77\u70b9\u5230\u7ec8\u70b9\u90fd\u4e0e\u53c2\u8003\u5b8c\u5168\u76f8\u540c\u3002\u82e5\u7814\u7a76\u95ee\u9898\u6d89\u53ca\u8f6c\u5f55\u8d77\u59cb\u6216\u7ec8\u6b62\u4f4d\u7f6e\uff0c\u5fc5\u987b\u7ee7\u7eed\u68c0\u67e5\u7aef\u70b9\u5b57\u6bb5\u548c\u72ec\u7acb\u8bc1\u636e\u3002<\/p>\n
\u7b2c\u4e8c\u6761\u7f3a\u5c11\u6700\u524d\u9762\u7684\u5916\u663e\u5b50\uff0c\u88ab\u5206\u7c7b\u4e3a\u4e0d\u5b8c\u6574\u526a\u63a5\u5339\u914d\uff0c\u5e76\u6807\u8bb0\u4e3a\u4e09\u7aef\u7247\u6bb5\u3002\u8fd9\u4e2a\u6807\u7b7e\u8bf4\u660e\u5b83\u8986\u76d6\u53c2\u8003\u7684\u4e00\u90e8\u5206\u7ed3\u6784\uff0c\u4e0d\u80fd\u5355\u72ec\u5224\u5b9a\u5b83\u6765\u81ea\u964d\u89e3\uff0c\u4e5f\u4e0d\u80fd\u76f4\u63a5\u8bc1\u660e\u5b83\u662f\u7a33\u5b9a\u5b58\u5728\u7684\u77ed\u8f6c\u5f55\u672c\u3002<\/p>\n
\u628a\u8fd9\u4e24\u6761\u653e\u5728\u4e00\u8d77\u770b\uff0c\u53ef\u4ee5\u533a\u5206\u526a\u63a5\u94fe\u5b8c\u6574\u7a0b\u5ea6\u4e0e\u7aef\u70b9\u4e00\u81f4\u7a0b\u5ea6\u3002\u524d\u8005\u5b8c\u6574\u4e0d\u4ee3\u8868\u7aef\u70b9\u4e00\u81f4\uff0c\u540e\u8005\u4e0d\u5b8c\u6574\u4e5f\u4e0d\u610f\u5473\u7740\u5269\u4f59\u8fde\u63a5\u5c31\u4e00\u5b9a\u6709\u95ee\u9898\u3002\u5b98\u65b9\u5206\u7c7b\u8bf4\u660e\u540c\u6837\u5c06\u8fd9\u4e9b\u5173\u7cfb\u5206\u5f00\u5b9a\u4e49\u3002SQANTI3 \u7ed3\u6784\u7c7b\u522b<\/a><\/p>\n
NIC \u4e0e NNC \u7684\u533a\u522b\u843d\u5728\u4ec0\u4e48\u4f4d\u7f6e<\/h2>\n
\u8df3\u8fc7\u4e2d\u95f4\u5916\u663e\u5b50\u7684\u7b2c\u4e09\u6761\u67e5\u8be2\uff0c\u4f7f\u7528\u7684\u4f9b\u4f53\u548c\u53d7\u4f53\u90fd\u5df2\u5b58\u5728\u4e8e\u53c2\u8003\uff0c\u4f46\u4e8c\u8005\u5f62\u6210\u7684\u76f4\u63a5\u8fde\u63a5\u4e0d\u5728\u53c2\u8003\u6a21\u578b\u4e2d\u3002\u5b9e\u9645\u5f97\u5230 NIC\uff0c\u5b50\u7c7b\u6307\u51fa\u8fd9\u662f\u5df2\u6709\u526a\u63a5\u4f4d\u70b9\u7684\u65b0\u7ec4\u5408\u3002<\/p>\n
\u7b2c\u56db\u6761\u67e5\u8be2\u4f7f\u7528\u65b0\u7684\u4f9b\u4f53\uff0c\u5c3d\u7ba1\u8fde\u63a5\u5e8f\u5217\u4ecd\u4e3a\u5178\u578b\u7ec4\u5408\uff0c\u4e5f\u5f97\u5230 NNC\u3002\u7b2c\u4e94\u6761\u540c\u6837\u4f7f\u7528\u65b0\u4f9b\u4f53\uff0c\u5e76\u4e14\u8fde\u63a5\u5e8f\u5217\u975e\u5178\u578b\uff0c\u540c\u6837\u5f97\u5230 NNC\u3002\u4e24\u8005\u7ed3\u6784\u7c7b\u522b\u76f8\u540c\uff0c\u8fde\u63a5\u5178\u578b\u6027\u5374\u4e0d\u540c\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n\n
\u67e5\u8be2\u8bbe\u8ba1<\/th>\n\u5b9e\u9645\u7c7b\u522b<\/th>\n\u8fde\u63a5\u60c5\u51b5<\/th>\n<\/tr>\n<\/thead>\n
\u5185\u90e8\u94fe\u4e00\u81f4\uff0c\u4e24\u7aef\u7f29\u77ed<\/td>\nFSM<\/td>\n\u5178\u578b<\/td>\n<\/tr>\n
\u7f3a\u9996\u4e2a\u5916\u663e\u5b50<\/td>\nISM<\/td>\n\u5178\u578b<\/td>\n<\/tr>\n
\u5df2\u6709\u4f4d\u70b9\u7684\u65b0\u8fde\u63a5<\/td>\nNIC<\/td>\n\u5178\u578b<\/td>\n<\/tr>\n
\u65b0\u4f9b\u4f53\uff0c\u8bbe\u7f6e GTAG<\/td>\nNNC<\/td>\n\u5178\u578b<\/td>\n<\/tr>\n
\u65b0\u4f9b\u4f53\uff0c\u8bbe\u7f6e TTAG<\/td>\nNNC<\/td>\n\u975e\u5178\u578b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u7531\u6b64\u53ef\u89c1\uff0c\u662f\u5426\u4e3a\u65b0\u4f4d\u70b9\u548c\u662f\u5426\u7b26\u5408\u5178\u578b\u8fde\u63a5\u6a21\u5f0f\u4e0d\u662f\u540c\u4e00\u4e2a\u7ef4\u5ea6\u3002\u4e0d\u80fd\u628a\u6240\u6709 NNC \u90fd\u5f53\u6210\u975e\u5178\u578b\u8fde\u63a5\uff0c\u4e5f\u4e0d\u80fd\u56e0\u4e3a\u4e00\u4e2a\u8fde\u63a5\u7b26\u5408\u5e38\u89c1\u6a21\u5f0f\uff0c\u5c31\u628a\u5b83\u5f53\u4f5c\u5df2\u7ecf\u88ab\u771f\u5b9e\u6837\u672c\u9a8c\u8bc1\u7684\u65b0\u7ed3\u6784\u3002<\/p>\n
\u8fde\u63a5\u8868\u8fd8\u80fd\u628a\u8fd9\u4e2a\u5dee\u522b\u8fdb\u4e00\u6b65\u62c6\u5f00\u3002\u7b2c\u4e09\u6761\u67e5\u8be2\u7684\u8fde\u63a5\u672c\u8eab\u6807\u8bb0\u4e3a\u65b0\uff0c\u4f46\u4e24\u7aef\u4f4d\u70b9\u90fd\u6807\u8bb0\u4e3a\u5df2\u77e5\u3002\u7b2c\u56db\u6761\u5219\u662f\u4e00\u7aef\u65b0\u3001\u4e00\u7aef\u5df2\u77e5\u3002\u6bd4\u8d77\u53ea\u4fdd\u7559\u603b\u7c7b\u522b\uff0c\u8fd9\u79cd\u5206\u89e3\u66f4\u80fd\u5e2e\u52a9\u89e3\u91ca\u5019\u9009\u5230\u5e95\u65b0\u5728\u54ea\u91cc\u3002<\/p>\n
\u53cd\u4e49\u3001\u5185\u542b\u5b50\u548c\u57fa\u56e0\u95f4\u533a\u4e5f\u4e0d\u662f\u8d28\u91cf\u7b49\u7ea7<\/h2>\n
\u53cd\u4e49\u94fe\u67e5\u8be2\u88ab\u5f52\u5165\u53cd\u4e49\u7c7b\u522b\uff0c\u5185\u542b\u5b50\u5185\u90e8\u7684\u5355\u5916\u663e\u5b50\u67e5\u8be2\u88ab\u5f52\u5165\u5185\u542b\u5b50\u533a\u57df\u7c7b\u522b\uff0c\u57fa\u56e0\u95f4\u533a\u5355\u5916\u663e\u5b50\u67e5\u8be2\u5219\u5f52\u5165\u57fa\u56e0\u95f4\u7c7b\u522b\u3002\u8fd9\u4e9b\u6807\u7b7e\u9996\u5148\u63cf\u8ff0\u76f8\u5bf9\u4e8e\u5f53\u524d\u53c2\u8003\u7684\u4f4d\u7f6e\u5173\u7cfb\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u67e5\u8be2\u8bbe\u8ba1<\/th>\n\u5b9e\u9645\u7c7b\u522b<\/th>\n\u672c\u6b21\u9700\u8981\u6ce8\u610f<\/th>\n<\/tr>\n<\/thead>\n
\u76f8\u540c\u533a\u57df\uff0c\u53cd\u5411\u94fe<\/td>\nantisense<\/td>\n\u65b9\u5411\u6539\u53d8\uff0c\u8fde\u63a5\u89e3\u91ca\u4e5f\u53d8\u5316<\/td>\n<\/tr>\n
\u4f4d\u4e8e\u53c2\u8003\u5185\u542b\u5b50\u5185\u90e8<\/td>\ngenic_intron<\/td>\n\u6ca1\u6709\u526a\u63a5\u8fde\u63a5<\/td>\n<\/tr>\n
\u4f4d\u4e8e\u53c2\u8003\u4e4b\u5916<\/td>\nintergenic<\/td>\n\u4e0b\u6e38\u4eba\u5de5\u8bbe\u7f6e\u4e3a\u5168 A<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u5355\u5916\u663e\u5b50\u67e5\u8be2\u6ca1\u6709\u53ef\u68c0\u67e5\u7684\u526a\u63a5\u8fde\u63a5\uff0c\u56e0\u6b64\u5178\u578b\u6027\u5b57\u6bb5\u4e3a\u7a7a\u3002\u8fd9\u4e2a\u7a7a\u503c\u4e0d\u80fd\u5f53\u6210\u975e\u5178\u578b\uff0c\u4e5f\u4e0d\u80fd\u5f53\u6210\u5df2\u7ecf\u901a\u8fc7\u5178\u578b\u8fde\u63a5\u68c0\u67e5\u3002\u6ca1\u6709\u76f8\u5e94\u5bf9\u8c61\u53ef\u8ba1\u7b97\uff0c\u4e0e\u8ba1\u7b97\u540e\u5f97\u5230\u67d0\u79cd\u7ed3\u679c\uff0c\u662f\u4e0d\u540c\u60c5\u51b5\u3002<\/p>\n
\u5b9e\u9645\u6570\u636e\u51fa\u73b0\u8fd9\u4e9b\u7c7b\u522b\u65f6\uff0c\u5e94\u68c0\u67e5\u6ce8\u91ca\u8986\u76d6\u7a0b\u5ea6\u3001\u94fe\u7279\u5f02\u4fe1\u606f\u3001\u6bd4\u5bf9\u4e0e\u8868\u8fbe\u652f\u6301\u3002\u53c2\u8003\u4e4b\u5916\u7684\u7ed3\u6784\u53ef\u80fd\u503c\u5f97\u7814\u7a76\uff0c\u4e5f\u53ef\u80fd\u6765\u81ea\u6280\u672f\u95ee\u9898\uff0c\u4ec5\u9760\u4f4d\u7f6e\u7c7b\u522b\u65e0\u6cd5\u5b8c\u6210\u771f\u5047\u5224\u65ad\u3002<\/p>\n
\u53ea\u52a0\u4e00\u6761\u53c2\u8003\uff0cNIC \u5c31\u53d8\u6210 FSM<\/h2>\n
\u7b2c\u4e8c\u7ec4\u6ca1\u6709\u6539\u52a8\u67e5\u8be2\u5e8f\u5217\u3001\u7ed3\u6784\u548c\u57fa\u56e0\u7ec4\uff0c\u53ea\u5728\u53c2\u8003\u4e2d\u589e\u52a0\u4e86\u7b2c\u4e09\u6761\u67e5\u8be2\u5bf9\u5e94\u7684\u8df3\u8dc3\u6a21\u578b\u3002\u8be5\u67e5\u8be2\u4ece NIC \u53d8\u4e3a\u5b8c\u6574\u526a\u63a5\u5339\u914d\uff0c\u5173\u8054\u8f6c\u5f55\u672c\u4e5f\u4ece\u65b0\u6a21\u578b\u6807\u8bb0\u53d8\u6210\u65b0\u589e\u7684\u53c2\u8003\u540d\u79f0\u3002<\/p>\n
\u8fd9\u4e2a\u53d8\u5316\u6ca1\u6709\u589e\u52a0\u4efb\u4f55\u5b9e\u9a8c\u652f\u6301\u3002\u5b83\u53ea\u6539\u53d8\u4e86\u67e5\u8be2\u76f8\u5bf9\u4e8e\u53c2\u8003\u96c6\u5408\u7684\u8eab\u4efd\u3002\u56e0\u6b64\uff0c\u6bd4\u8f83\u4e0d\u540c\u9879\u76ee\u7684\u65b0\u8f6c\u5f55\u672c\u6bd4\u4f8b\u65f6\uff0c\u5fc5\u987b\u540c\u65f6\u6838\u5bf9\u53c2\u8003\u7248\u672c\u3001\u7eb3\u5165\u7684\u8f6c\u5f55\u672c\u8303\u56f4\u548c\u5904\u7406\u6d41\u7a0b\u3002<\/p>\n
\u5982\u679c\u4e00\u4e2a\u9879\u76ee\u4f7f\u7528\u66f4\u4e30\u5bcc\u7684\u53c2\u8003\u96c6\u5408\uff0c\u65b0\u7c7b\u522b\u6bd4\u4f8b\u53ef\u80fd\u4e0b\u964d\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u6d4b\u5e8f\u6570\u636e\u66f4\u4fdd\u5b88\u3002\u53cd\u8fc7\u6765\uff0c\u4f7f\u7528\u8f83\u65e7\u6216\u8f83\u7cbe\u7b80\u7684\u6ce8\u91ca\uff0c\u53ef\u80fd\u628a\u5df2\u6709\u7ed3\u6784\u91cd\u65b0\u6807\u8bb0\u4e3a\u65b0\u3002\u5355\u72ec\u6bd4\u8f83\u6bd4\u4f8b\uff0c\u5bb9\u6613\u628a\u6ce8\u91ca\u5dee\u5f02\u8bb2\u6210\u751f\u7269\u5b66\u5dee\u5f02\u3002<\/p>\n
\u5b9e\u9645\u5f52\u6863\u65f6\uff0c\u5e94\u4fdd\u5b58\u53c2\u8003\u6587\u4ef6\u672c\u8eab\u6216\u53ef\u6838\u9a8c\u7684\u7248\u672c\u4e0e\u6458\u8981\uff0c\u4e0d\u80fd\u53ea\u5199\u4e00\u4e2a\u7269\u79cd\u540d\u79f0\u3002\u540e\u7eed\u5347\u7ea7\u53c2\u8003\uff0c\u518d\u8ffd\u8e2a\u54ea\u4e9b\u5019\u9009\u53d1\u751f\u7c7b\u522b\u53d8\u5316\uff0c\u6bd4\u76f4\u63a5\u8986\u76d6\u65e7\u7ed3\u679c\u66f4\u6709\u52a9\u4e8e\u89e3\u91ca\u7ed3\u8bba\u6f14\u53d8\u3002<\/p>\n
non_coding \u53ef\u80fd\u610f\u5473\u7740\u672c\u6b21\u6839\u672c\u6ca1\u6709\u9884\u6d4b<\/h2>\n
\u672c\u5b9e\u9a8c\u6ca1\u6709\u5f00\u542f ORF \u9884\u6d4b\uff0c\u516b\u6761\u67e5\u8be2\u7684\u7f16\u7801\u5b57\u6bb5\u5374\u5168\u90e8\u5199\u6210\u975e\u7f16\u7801\uff0c\u76f8\u5173\u957f\u5ea6\u3001\u4f4d\u7f6e\u548c\u8bc4\u5206\u5b57\u6bb5\u5219\u4e3a\u7a7a\u3002\u65e5\u5fd7\u540c\u65f6\u660e\u786e\u5199\u51fa\u8df3\u8fc7\u9884\u6d4b\u3002\u8fd9\u662f\u4e00\u4e2a\u4e0d\u80fd\u5ffd\u7565\u7684\u7ed3\u679c\u89e3\u91ca\u9677\u9631\u3002<\/p>\n
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