{"id":73148,"date":"2026-11-15T14:14:00","date_gmt":"2026-11-15T06:14:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73148"},"modified":"2026-11-15T14:14:00","modified_gmt":"2026-11-15T06:14:00","slug":"rmats-psi-effective-length-replicates-missing-value-result-review","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/rmats-psi-effective-length-replicates-missing-value-result-review\/","title":{"rendered":"rMATS-turbo \u7ed3\u679c\u600e\u4e48\u8bfb\uff0c\u5305\u542b\u8bfb\u6bb5\u5360\u4e09\u5206\u4e4b\u4e8c\uff0c\u4e3a\u4ec0\u4e48 PSI \u53ea\u6709\u4e00\u534a"},"content":{"rendered":"
\n

\u5dee\u5f02\u526a\u63a5\u5206\u6790\u6700\u5bb9\u6613\u88ab\u7b80\u5316\u6210\u4e00\u9879\u7b5b\u9009\u64cd\u4f5c\uff0c\u6253\u5f00\u7ed3\u679c\u8868\uff0c\u4fdd\u7559\u663e\u8457\u884c\uff0c\u518d\u628a\u5bf9\u5e94\u57fa\u56e0\u9001\u53bb\u5bcc\u96c6\u3002\u4f46\u5982\u679c\u5305\u542b\u6c34\u5e73\u3001\u7ec4\u522b\u65b9\u5411\u3001\u91cd\u590d\u4e4b\u95f4\u7684\u5dee\u5f02\u548c\u8ba1\u6570\u53e3\u5f84\u6ca1\u6709\u8bfb\u6e05\u695a\uff0c\u7b5b\u51fa\u6765\u7684\u5217\u8868\u53ef\u80fd\u5728\u7b2c\u4e00\u6b65\u5c31\u5e26\u7740\u9519\u8bef\u89e3\u91ca\u3002<\/p>\n

\u672c\u7bc7\u56f4\u7ed5 rMATS-turbo \u7684\u7ed3\u679c\u542b\u4e49\uff0c\u4f7f\u7528\u56db\u7ec4\u539f\u521b\u865a\u6784\u8ba1\u6570\u7ec3\u4e60\uff0c\u5b9e\u9645\u8fd0\u884c\u72ec\u7acb Python \u811a\u672c\u6838\u5bf9\u8ba1\u7b97\u3002\u6ca1\u6709\u5b89\u88c5\u6216\u6267\u884c rMATS-turbo\uff0c\u4e5f\u6ca1\u6709\u8fdb\u884c\u4e8b\u4ef6\u53d1\u73b0\u548c\u7edf\u8ba1\u62df\u5408\u3002\u6587\u4e2d\u7684\u6821\u6b63\u663e\u8457\u6027\u6570\u503c\u5747\u4e3a\u8bfb\u8868\u8bad\u7ec3\u800c\u624b\u5de5\u8bbe\u7f6e\uff0c\u4e0d\u80fd\u4f5c\u4e3a\u8f6f\u4ef6\u5b9e\u6d4b\u7ed3\u679c\u3002<\/p>\n

\"\u516d\u6210\u4e03\u7684\u8bfb\u6bb5\uff0c\u4e0d\u7b49\u4e8e\u516d\u6210\u4e03
\u56db\u7ec4\u539f\u521b\u8ba1\u6570\u7ec3\u4e60\uff0c\u6838\u5bf9\u6bd4\u4f8b\u3001\u91cd\u590d\u548c\u7f3a\u5931<\/figcaption><\/figure>\n

\u5148\u786e\u8ba4\u5bf9\u8c61\u662f\u526a\u63a5\u4e8b\u4ef6<\/h2>\n

\u4e00\u4e2a\u57fa\u56e0\u53ef\u4ee5\u6709\u591a\u4e2a\u53ef\u53d8\u533a\u57df\uff0c\u540c\u4e00\u53ef\u53d8\u533a\u57df\u4e5f\u53ef\u80fd\u53c2\u4e0e\u4e0d\u540c\u7ed3\u6784\u5173\u7cfb\u3002\u4e8b\u4ef6\u7ea7\u7ed3\u679c\u56e0\u6b64\u4e0d\u7b49\u4e8e\u57fa\u56e0\u7ea7\u8868\u8fbe\u7ed3\u679c\u3002\u67d0\u4e2a\u4e8b\u4ef6\u7684\u5305\u542b\u6c34\u5e73\u53d8\u5316\uff0c\u4e0d\u80fd\u76f4\u63a5\u6539\u5199\u6210\u6574\u4e2a\u57fa\u56e0\u8868\u8fbe\u4e0a\u8c03\u6216\u4e0b\u8c03\u3002<\/p>\n

\u4f8b\u5982\uff0c\u4e00\u4e2a\u5916\u663e\u5b50\u8df3\u8dc3\u4e8b\u4ef6\u7684\u5305\u542b\u5f62\u5f0f\u4fdd\u7559\u76ee\u6807\u5916\u663e\u5b50\uff0c\u53e6\u4e00\u79cd\u5f62\u5f0f\u8de8\u8fc7\u5b83\u3002\u5305\u542b\u6bd4\u4f8b\u589e\u52a0\uff0c\u63cf\u8ff0\u7684\u662f\u8fd9\u4e24\u7c7b\u5c40\u90e8\u9009\u62e9\u7684\u76f8\u5bf9\u53d8\u5316\u3002\u603b\u8f6c\u5f55\u91cf\u53ef\u80fd\u589e\u52a0\u3001\u4e0b\u964d\u6216\u8005\u4fdd\u6301\u4e0d\u53d8\uff0c\u5355\u72ec\u7684\u6bd4\u4f8b\u5e76\u4e0d\u80fd\u56de\u7b54\u3002<\/p>\n

\u8fdb\u4e00\u6b65\u505a\u57fa\u56e0\u6c47\u603b\u65f6\uff0c\u4e5f\u8981\u5148\u660e\u786e\u4e00\u884c\u4e8b\u4ef6\u662f\u5426\u4ee3\u8868\u72ec\u7acb\u8bc1\u636e\u3002\u540c\u4e00\u57fa\u56e0\u51fa\u73b0\u591a\u884c\uff0c\u4e0d\u5e94\u76f4\u63a5\u7d2f\u8ba1\u6210\u591a\u4e2a\u72ec\u7acb\u9a8c\u8bc1\u3002\u5982\u679c\u53ea\u9700\u8981\u4e00\u4e2a\u5019\u9009\u57fa\u56e0\u5217\u8868\uff0c\u53ef\u4ee5\u53bb\u91cd\uff0c\u4f46\u540c\u65f6\u5e94\u4fdd\u7559\u4e8b\u4ef6\u5750\u6807\u548c\u53d8\u5316\u65b9\u5411\u4f9b\u56de\u67e5\u3002<\/p>\n

rMATS \u7684\u65b9\u6cd5\u8bbe\u8ba1\u5c06\u8bfb\u6bb5\u62bd\u6837\u4e0d\u786e\u5b9a\u6027\u548c\u91cd\u590d\u4e4b\u95f4\u7684\u53d8\u5316\u7eb3\u5165\u8003\u8651\u3002\u539f\u59cb\u7814\u7a76\u4e5f\u8ba8\u8bba\u4e86\u7b80\u5355\u5408\u5e76\u91cd\u590d\u4e0d\u80fd\u66ff\u4ee3\u91cd\u590d\u5c42\u9762\u5efa\u6a21\u7684\u95ee\u9898\u3002rMATS \u539f\u59cb\u7814\u7a76<\/a><\/p>\n

\u8fd9\u91cc\u4e3a\u4ec0\u4e48\u91c7\u7528\u72ec\u7acb\u8bfb\u8868\u7ec3\u4e60<\/h2>\n

\u9879\u76ee\u5f53\u524d\u8bb8\u53ef\u8bf4\u660e\u9762\u5411\u5b66\u672f\u548c\u975e\u8425\u5229\u7814\u7a76\uff0c\u5e76\u8981\u6c42\u5546\u4e1a\u5b9e\u4f53\u4f7f\u7528\u65f6\u53e6\u884c\u8054\u7cfb\u3002\u5728\u6ca1\u6709\u786e\u8ba4\u8986\u76d6\u672c\u6b21\u7528\u9014\u7684\u6388\u6743\u524d\uff0c\u6211\u4eec\u53ea\u67e5\u9605\u516c\u5f00\u8d44\u6599\uff0c\u8fd0\u884c\u81ea\u5df1\u7f16\u5199\u7684\u7b97\u672f\u68c0\u67e5\uff0c\u4e0d\u4e0b\u8f7d\u8f6f\u4ef6\u7ed5\u8fc7\u8bb8\u53ef\uff0c\u4e5f\u4e0d\u628a\u7ec3\u4e60\u79f0\u4f5c\u5b89\u88c5\u5b9e\u8dd1\u3002rMATS-turbo \u5b98\u65b9\u8bb8\u53ef<\/a><\/p>\n

\u8fd9\u4e0d\u5f71\u54cd\u5b66\u4e60\u7ed3\u679c\u89e3\u91ca\u3002\u4e0b\u9762\u6bcf\u4e2a\u4f8b\u5b50\u7684\u8f93\u5165\u90fd\u516c\u5f00\uff0c\u8bfb\u8005\u53ef\u4ee5\u624b\u7b97\uff0c\u4e5f\u53ef\u4ee5\u8fd0\u884c\u9644\u4ef6\u811a\u672c\u590d\u6838\u3002\u7ec3\u4e60\u4e0d\u4f1a\u590d\u73b0 rMATS \u7684\u7edf\u8ba1\u6a21\u578b\uff0c\u4e0d\u751f\u6210\u771f\u5b9e\u68c0\u9a8c\u6982\u7387\uff0c\u66f4\u4e0d\u80fd\u66ff\u4ee3\u6b63\u5f0f\u5206\u6790\u6240\u9700\u7684\u6bd4\u5bf9\u548c\u751f\u7269\u5b66\u91cd\u590d\u3002<\/p>\n

\u6211\u4eec\u56fa\u5b9a\u5305\u542b\u5f62\u5f0f\u7684\u6709\u6548\u957f\u5ea6\u4e3a\u4e8c\u767e\uff0c\u8df3\u8dc3\u5f62\u5f0f\u4e3a\u4e00\u767e\uff0c\u7528\u6765\u9694\u79bb\u957f\u5ea6\u5f52\u4e00\u5316\u7684\u5f71\u54cd\u3002\u8fd9\u4e9b\u957f\u5ea6\u662f\u7ec3\u4e60\u8bbe\u5b9a\uff0c\u4e0d\u80fd\u76f4\u63a5\u590d\u5236\u5230\u81ea\u5df1\u7684\u6240\u6709\u4e8b\u4ef6\u4e2d\u3002\u771f\u5b9e\u6587\u4ef6\u5e94\u4f7f\u7528\u5bf9\u5e94\u4e8b\u4ef6\u548c\u8ba1\u6570\u65b9\u5f0f\u7ed9\u51fa\u7684\u5b9e\u9645\u957f\u5ea6\u3002<\/p>\n

\u7b2c\u4e00\u8bfe\uff0c\u8bfb\u6bb5\u6bd4\u4f8b\u4e0d\u7b49\u4e8e\u5305\u542b\u6c34\u5e73<\/h2>\n

\u7b2c\u4e00\u7ec4\u7ec3\u4e60\u7684\u4e00\u4e2a\u6837\u672c\u6709\u56db\u5341\u6761\u5305\u542b\u652f\u6301\u548c\u4e8c\u5341\u6761\u8df3\u8dc3\u652f\u6301\u3002\u5982\u679c\u76f4\u63a5\u7b97\u56db\u5341\u9664\u4ee5\u516d\u5341\uff0c\u5f97\u5230\u7ea6\u4e09\u5206\u4e4b\u4e8c\u3002\u4f46\u4e24\u79cd\u5f62\u5f0f\u63d0\u4f9b\u88ab\u8ba1\u6570\u673a\u4f1a\u7684\u6709\u6548\u957f\u5ea6\u4e0d\u540c\uff0c\u9700\u8981\u5148\u6821\u6b63\u8fd9\u4e2a\u5dee\u522b\u3002<\/p>\n

\u628a\u56db\u5341\u9664\u4ee5\u4e8c\u767e\uff0c\u5f97\u5230\u96f6\u70b9\u4e8c\u3002\u628a\u4e8c\u5341\u9664\u4ee5\u4e00\u767e\uff0c\u4e5f\u5f97\u5230\u96f6\u70b9\u4e8c\u3002\u968f\u540e\u7528\u524d\u8005\u9664\u4ee5\u4e24\u8005\u4e4b\u548c\uff0c\u7ed3\u679c\u4e3a\u96f6\u70b9\u4e94\u3002\u4e5f\u5c31\u662f\u8bf4\uff0c\u539f\u59cb\u5305\u542b\u8bfb\u6bb5\u6bd4\u4f8b\u7ea6\u4e3a\u516d\u6210\u4e03\uff0c\u7ecf\u8fc7\u8fd9\u7ec4\u957f\u5ea6\u8bbe\u5b9a\u6821\u6b63\u540e\u7684\u5305\u542b\u6c34\u5e73\u662f\u4e00\u534a\u3002<\/p>\n

\u5305\u542b\u6807\u51c6\u5316\u652f\u6301 = I \/ L_I\r\n\u8df3\u8dc3\u6807\u51c6\u5316\u652f\u6301 = S \/ L_S\r\nPSI = (I \/ L_I) \/ ((I \/ L_I) + (S \/ L_S))\r\n\r\n\u672c\u4f8b\r\nI = 40\uff0cS = 20\uff0cL_I = 200\uff0cL_S = 100\r\nPSI = 0.2 \/ (0.2 + 0.2) = 0.5\r\n<\/code><\/pre>\n
\u8fd9\u9879\u8ba1\u7b97\u5e76\u4e0d\u662f\u628a\u8bfb\u6bb5\u6570\u968f\u610f\u6253\u6298\uff0c\u800c\u662f\u5728\u6bd4\u8f83\u4e24\u79cd\u5f62\u5f0f\u7684\u76f8\u5bf9\u652f\u6301\u65f6\u8003\u8651\u673a\u4f1a\u7a7a\u95f4\u3002\u82e5\u4e24\u4e2a\u957f\u5ea6\u6070\u597d\u76f8\u540c\uff0c\u539f\u59cb\u6bd4\u4f8b\u4e0e\u5f52\u4e00\u5316\u6bd4\u4f8b\u624d\u4f1a\u4e00\u81f4\u3002\u4e0d\u80fd\u56e0\u4e3a\u67d0\u4e00\u4e2a\u4f8b\u5b50\u4e2d\u4e24\u8005\u63a5\u8fd1\uff0c\u5c31\u6c38\u4e45\u7701\u7565\u957f\u5ea6\u9879\u3002<\/p>\n
\u4e5f\u4e0d\u8981\u628a\u6709\u6548\u957f\u5ea6\u7406\u89e3\u4e3a\u76ee\u6807\u5916\u663e\u5b50\u7684\u7269\u7406\u957f\u5ea6\u3002\u5b83\u670d\u52a1\u4e8e\u5f53\u524d\u8ba1\u6570\u6a21\u578b\uff0c\u548c\u4e8b\u4ef6\u7c7b\u578b\u3001\u8bfb\u957f\u53ca\u8ba1\u6570\u533a\u57df\u6709\u5173\u3002\u68c0\u67e5\u7ed3\u679c\u65f6\uff0c\u5e94\u5c06\u5305\u542b\u8ba1\u6570\u3001\u8df3\u8dc3\u8ba1\u6570\u548c\u4e24\u79cd\u6709\u6548\u957f\u5ea6\u4f5c\u4e3a\u4e00\u7ec4\u8bfb\u53d6\u3002<\/p>\n
\u91cd\u590d\u5217\u5fc5\u987b\u4fdd\u6301\u539f\u6765\u7684\u6837\u672c\u987a\u5e8f<\/h2>\n
\u7b2c\u4e00\u7ec4\u865a\u6784\u4e8b\u4ef6\u6709\u4e09\u4efd\u7ec4\u4e00\u8f93\u5165\uff0c\u5305\u542b\u4e0e\u8df3\u8dc3\u8ba1\u6570\u4f9d\u6b21\u4e3a\u56db\u5341\u4e0e\u4e8c\u5341\u3001\u56db\u5341\u56db\u4e0e\u4e8c\u5341\u4e8c\u3001\u4e09\u5341\u516d\u4e0e\u5341\u516b\u3002\u7ec4\u4e8c\u5206\u522b\u4e3a\u4e8c\u5341\u4e0e\u56db\u5341\u3001\u4e8c\u5341\u4e8c\u4e0e\u56db\u5341\u56db\u3001\u5341\u516b\u4e0e\u4e09\u5341\u516d\u3002<\/p>\n
\u4e09\u4efd\u7ec4\u4e00\u7684\u5f52\u4e00\u5316\u5305\u542b\u6c34\u5e73\u90fd\u4e3a\u96f6\u70b9\u4e94\uff0c\u4e09\u4efd\u7ec4\u4e8c\u90fd\u4e3a\u96f6\u70b9\u4e8c\u3002\u7ec3\u4e60\u4e2d\u7ec4\u4e00\u51cf\u7ec4\u4e8c\u5f97\u5230\u6b63\u96f6\u70b9\u4e09\uff0c\u8868\u793a\u7ec4\u4e00\u7684\u5c40\u90e8\u5305\u542b\u6c34\u5e73\u9ad8\u4e09\u5341\u4e2a\u767e\u5206\u70b9\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
\u7ec3\u4e60\u6837\u672c<\/th>\n\u5305\u542b\u8ba1\u6570<\/th>\n\u8df3\u8dc3\u8ba1\u6570<\/th>\nPSI<\/th>\n<\/tr>\n<\/thead>\n
\u7ec4\u4e00\uff0c\u7b2c 1 \u4efd<\/td>\n40<\/td>\n20<\/td>\n0.50<\/td>\n<\/tr>\n
\u7ec4\u4e00\uff0c\u7b2c 2 \u4efd<\/td>\n44<\/td>\n22<\/td>\n0.50<\/td>\n<\/tr>\n
\u7ec4\u4e8c\uff0c\u7b2c 1 \u4efd<\/td>\n20<\/td>\n40<\/td>\n0.20<\/td>\n<\/tr>\n
\u7ec4\u4e8c\uff0c\u7b2c 2 \u4efd<\/td>\n22<\/td>\n44<\/td>\n0.20<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u771f\u5b9e\u7ed3\u679c\u4e2d\uff0c\u91cd\u590d\u503c\u4ee5\u5217\u8868\u5f62\u5f0f\u4fdd\u5b58\u65f6\uff0c\u5fc5\u987b\u4e0e\u8f93\u5165\u5206\u7ec4\u6587\u4ef6\u7684\u987a\u5e8f\u5bf9\u5e94\u3002\u7ed9\u5217\u52a0\u6837\u672c\u540d\u79f0\u4e4b\u524d\uff0c\u5e94\u5148\u4fdd\u5b58\u4e00\u4efd\u7f16\u53f7\u5230\u6837\u672c\u7684\u6620\u5c04\uff0c\u907f\u514d\u56e0\u4e3a\u6392\u5e8f\u65b9\u5f0f\u53d8\u5316\u800c\u628a\u652f\u6301\u91cf\u5173\u8054\u5230\u53e6\u4e00\u4e2a\u6837\u672c\u3002<\/p>\n
\u5f53\u524d\u5b98\u65b9\u8f93\u51fa\u5b9a\u4e49\u7684\u5dee\u503c\u65b9\u5411\u662f\u7ec4\u4e00\u5e73\u5747\u5305\u542b\u6c34\u5e73\u51cf\u53bb\u7ec4\u4e8c\u5e73\u5747\u5305\u542b\u6c34\u5e73\u3002\u6539\u53d8\u4e24\u7ec4\u987a\u5e8f\u4f1a\u6539\u53d8\u7b26\u53f7\uff0c\u4f46\u4e0d\u81ea\u52a8\u6539\u53d8\u6548\u5e94\u7edd\u5bf9\u5927\u5c0f\u3002\u5199\u7ed3\u679c\u65f6\u5e94\u6ce8\u660e\u54ea\u4e2a\u751f\u7269\u5b66\u6761\u4ef6\u5bf9\u5e94\u7ec4\u4e00\uff0c\u800c\u4e0d\u662f\u53ea\u5199\u6b63\u5411\u6216\u8d1f\u5411\u3002rMATS-turbo \u8f93\u51fa\u8bf4\u660e<\/a><\/p>\n
\u7b2c\u4e8c\u8bfe\uff0c\u5408\u5e76\u8ba1\u6570\u4e0e\u5e73\u5747\u6bd4\u4f8b\u4e0d\u662f\u540c\u4e00\u4e2a\u95ee\u9898<\/h2>\n
\u7b2c\u4e8c\u4e2a\u865a\u6784\u4e8b\u4ef6\u6545\u610f\u8bbe\u7f6e\u4e0d\u5747\u8861\u8986\u76d6\u3002\u7ec4\u4e09\u4efd\u6837\u672c\u7684\u5305\u542b\u4e0e\u8df3\u8dc3\u8ba1\u6570\u5206\u522b\u4e3a\u4e8c\u4e0e\u96f6\u3001\u96f6\u4e0e\u5341\u516b\u3001\u96f6\u4e0e\u5341\u516b\u3002\u5f52\u4e00\u5316\u6bd4\u4f8b\u5206\u522b\u662f\u4e00\u3001\u96f6\u3001\u96f6\uff0c\u4e09\u4e2a\u6837\u672c\u7684\u7b97\u672f\u5e73\u5747\u4e3a\u4e09\u5206\u4e4b\u4e00\u3002<\/p>\n
\u5982\u679c\u5148\u5408\u5e76\u8ba1\u6570\uff0c\u5f97\u5230\u5305\u542b\u4e8c\u3001\u8df3\u8dc3\u4e09\u5341\u516d\u3002\u7528\u540c\u6837\u7684\u6709\u6548\u957f\u5ea6\u8ba1\u7b97\uff0c\u5408\u5e76\u6bd4\u4f8b\u7ea6\u4e3a\u96f6\u70b9\u96f6\u4e8c\u4e03\u3002\u4e09\u5206\u4e4b\u4e00\u548c\u767e\u5206\u4e4b\u4e8c\u70b9\u4e03\u5dee\u5f97\u5f88\u8fdc\uff0c\u4f46\u4e24\u6b21\u7b97\u672f\u90fd\u6ca1\u6709\u7b97\u9519\uff0c\u5b83\u4eec\u7ed9\u4e0d\u540c\u6837\u672c\u5206\u914d\u4e86\u4e0d\u540c\u6743\u91cd\u3002<\/p>\n
\u524d\u8005\u628a\u6bcf\u4efd\u6837\u672c\u7684\u6bd4\u4f8b\u7b49\u6743\u5e73\u5747\uff0c\u540e\u8005\u8ba9\u652f\u6301\u91cf\u8f83\u5927\u7684\u6837\u672c\u5728\u6574\u4f53\u6bd4\u4f8b\u4e2d\u5360\u66f4\u591a\u6743\u91cd\u3002\u8bfb\u8005\u5fc5\u987b\u5148\u77e5\u9053\u81ea\u5df1\u8981\u63cf\u8ff0\u7684\u662f\u5178\u578b\u6837\u672c\uff0c\u8fd8\u662f\u5408\u5e76\u8bc1\u636e\u96c6\u5408\uff0c\u800c\u4e0d\u80fd\u628a\u4e24\u79cd\u7ed3\u679c\u5f53\u6210\u53ef\u4e92\u6362\u7684\u7edf\u8ba1\u91cf\u3002<\/p>\n
\u672c\u7ec3\u4e60\u7684\u7ec4\u4e8c\u4e09\u4efd\u6837\u672c\u90fd\u4e3a\u96f6\u70b9\u4e94\u3002\u56e0\u6b64\uff0c\u6309\u6837\u672c\u5e73\u5747\u8ba1\u7b97\u7684\u5dee\u503c\u7ea6\u4e3a\u8d1f\u96f6\u70b9\u4e00\u516d\u4e03\uff0c\u5408\u5e76\u540e\u8ba1\u7b97\u7ea6\u4e3a\u8d1f\u96f6\u70b9\u56db\u4e03\u4e09\u3002\u4e00\u4e2a\u6c47\u603b\u65b9\u6cd5\u7684\u53d8\u5316\uff0c\u5c31\u80fd\u660e\u663e\u6539\u53d8\u5bf9\u6548\u5e94\u5927\u5c0f\u7684\u76f4\u89c2\u5224\u65ad\u3002<\/p>\n
\u8fd9\u5e76\u4e0d\u610f\u5473\u7740\u5e94\u5f53\u76f2\u76ee\u63a5\u53d7\u4f4e\u8986\u76d6\u6837\u672c\u7684\u6781\u7aef\u6bd4\u4f8b\u3002\u7b2c\u4e00\u4efd\u53ea\u6709\u4e24\u6761\u603b\u652f\u6301\uff0c\u6bd4\u4f8b\u4e3a\u4e00\u4f9d\u7136\u7f3a\u5c11\u7a33\u5b9a\u8bc1\u636e\u3002\u6b63\u786e\u505a\u6cd5\u662f\u540c\u65f6\u5c55\u793a\u652f\u6301\u91cf\u548c\u91cd\u590d\u5206\u5e03\uff0c\u518d\u4f7f\u7528\u5408\u9002\u7684\u6b63\u5f0f\u6a21\u578b\uff0c\u800c\u4e0d\u662f\u901a\u8fc7\u5408\u5e76\u628a\u95ee\u9898\u9690\u85cf\u8d77\u6765\u3002<\/p>\n
\u7b2c\u4e09\u8bfe\uff0c\u6ca1\u6709\u652f\u6301\u4e0e\u5b8c\u5168\u8df3\u8dc3\u5fc5\u987b\u5206\u5f00<\/h2>\n
\u7b2c\u4e09\u4e2a\u865a\u6784\u4e8b\u4ef6\u7684\u7ec4\u4e00\u7b2c\u4e00\u4efd\u6837\u672c\uff0c\u5305\u542b\u548c\u8df3\u8dc3\u8ba1\u6570\u90fd\u4e3a\u96f6\u3002\u6b64\u65f6\u8ba1\u7b97\u5f0f\u7684\u5206\u5b50\u4e0e\u5206\u6bcd\u90fd\u662f\u96f6\uff0c\u5305\u542b\u6c34\u5e73\u6ca1\u6709\u5b9a\u4e49\u3002\u5b83\u65e2\u4e0d\u662f\u5b8c\u5168\u5305\u542b\uff0c\u4e5f\u4e0d\u662f\u5b8c\u5168\u8df3\u8dc3\u3002<\/p>\n
\u5269\u4f59\u4e24\u4efd\u6837\u672c\u7684\u6bd4\u4f8b\u90fd\u662f\u96f6\u70b9\u4e94\u3002\u82e5\u53ea\u63cf\u8ff0\u6709\u89c2\u5bdf\u503c\u7684\u6837\u672c\uff0c\u5747\u503c\u662f\u96f6\u70b9\u4e94\u3002\u82e5\u628a\u7f3a\u5931\u503c\u586b\u6210\u96f6\u518d\u5e73\u5747\uff0c\u5747\u503c\u4f1a\u964d\u5230\u4e09\u5206\u4e4b\u4e00\u3002\u540e\u8005\u628a\u6ca1\u6709\u89c2\u5bdf\u5230\u4e8b\u4ef6\u7684\u4fe1\u606f\uff0c\u6539\u5199\u6210\u89c2\u5bdf\u5230\u4e86\u5b8c\u5168\u8df3\u8dc3\u3002<\/p>\n
\u672c\u7ec3\u4e60\u660e\u786e\u91c7\u7528\u5bf9\u5df2\u89c2\u5bdf\u503c\u8ba1\u7b97\u5747\u503c\u7684\u7ea6\u5b9a\uff0c\u540c\u65f6\u53e6\u5916\u6807\u8bb0\u662f\u5426\u6240\u6709\u91cd\u590d\u90fd\u6709\u7ed3\u679c\u3002\u8fd9\u4e2a\u7ea6\u5b9a\u4ec5\u7528\u4e8e\u5c55\u793a\u7b97\u672f\u5dee\u5f02\uff0c\u5e76\u4e0d\u662f\u58f0\u79f0 rMATS \u5728\u6240\u6709\u7248\u672c\u548c\u573a\u666f\u4e0b\u90fd\u4f1a\u8fd9\u6837\u5904\u7406\u7f3a\u5931\u503c\u3002<\/p>\n
\u6b63\u5f0f\u6574\u7406\u7ed3\u679c\u65f6\uff0c\u5e94\u4fdd\u7559\u7f3a\u5931\u72b6\u6001\uff0c\u6838\u5bf9\u8986\u76d6\u3001\u53ef\u7528\u8bfb\u6bb5\u548c\u6d41\u7a0b\u65e5\u5fd7\u3002\u6ca1\u6709\u652f\u6301\u53ef\u80fd\u4e0e\u5c40\u90e8\u8868\u8fbe\u4f4e\u3001\u6bd4\u5bf9\u6216\u8fc7\u6ee4\u7b49\u56e0\u7d20\u6709\u5173\uff0c\u4e0d\u80fd\u53ea\u7528\u4e00\u4e2a\u96f6\u5c31\u628a\u539f\u56e0\u62b9\u6389\u3002<\/p>\n
\u5982\u679c\u4e0b\u6e38\u7ed8\u56fe\u6216\u7b5b\u9009\u51fd\u6570\u4e0d\u63a5\u53d7\u7f3a\u5931\u503c\uff0c\u5e94\u660e\u786e\u9009\u62e9\u5904\u7406\u89c4\u5219\uff0c\u5e76\u62a5\u544a\u54ea\u4e9b\u4e8b\u4ef6\u56e0\u6b64\u88ab\u6392\u9664\u3002\u6280\u672f\u4e0a\u8ba9\u51fd\u6570\u987a\u5229\u6267\u884c\uff0c\u4e0d\u7b49\u4e8e\u8fd9\u4e2a\u7f3a\u5931\u503c\u5df2\u7ecf\u83b7\u5f97\u5408\u7406\u7684\u751f\u7269\u5b66\u89e3\u91ca\u3002<\/p>\n
\u7b2c\u56db\u8bfe\uff0c\u663e\u8457\u6027\u548c\u6548\u5e94\u5927\u5c0f\u9700\u8981\u4e00\u8d77\u8bfb<\/h2>\n
\u7b2c\u56db\u4e2a\u865a\u6784\u4e8b\u4ef6\u7ed9\u4e24\u7ec4\u8bbe\u7f6e\u5f88\u9ad8\u7684\u652f\u6301\u91cf\uff0c\u4f46\u5e73\u5747\u5305\u542b\u6c34\u5e73\u53ea\u4ece\u96f6\u70b9\u4e94\u53d8\u6210\u96f6\u70b9\u4e94\u4e00\uff0c\u5dee\u5f02\u4e3a\u4e00\u4e2a\u767e\u5206\u70b9\u3002\u6211\u4eec\u624b\u5de5\u7ed9\u5b83\u8bbe\u7f6e\u4e00\u4e2a\u5f88\u5c0f\u7684\u6821\u6b63\u663e\u8457\u6027\u503c\uff0c\u7528\u6765\u7ec3\u4e60\u8bc6\u522b\u53d8\u5316\u5f88\u5c0f\u4f46\u7edf\u8ba1\u6807\u7b7e\u5f88\u9192\u76ee\u7684\u60c5\u5f62\u3002<\/p>\n
\u8fd9\u4e2a\u6570\u503c\u4e0d\u662f\u4ece\u5f53\u524d\u8ba1\u6570\u8ba1\u7b97\u51fa\u6765\u7684\uff0c\u4e5f\u6ca1\u6709\u7ecf\u8fc7\u8f6f\u4ef6\u62df\u5408\u3002\u7ec3\u4e60\u7684\u76ee\u7684\uff0c\u662f\u8ba9\u8bfb\u8005\u533a\u5206\u53d8\u5316\u5e45\u5ea6\u548c\u4e0d\u786e\u5b9a\u6027\u8fd9\u4e24\u4e2a\u95ee\u9898\u3002\u771f\u5b9e\u5206\u6790\u4e2d\uff0c\u662f\u5426\u503c\u5f97\u8fdb\u4e00\u6b65\u9a8c\u8bc1\uff0c\u8fd8\u9700\u8981\u7ed3\u5408\u7814\u7a76\u76ee\u6807\u548c\u53ef\u68c0\u6d4b\u7684\u53d8\u5316\u8303\u56f4\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
\u865a\u6784\u4e8b\u4ef6<\/th>\n\u5e73\u5747\u5dee\u503c<\/th>\n\u9884\u8bbe FDR<\/th>\n\u672c\u7ec3\u4e60\u68c0\u67e5\u91cd\u70b9<\/th>\n<\/tr>\n<\/thead>\n
\u957f\u5ea6\u5f52\u4e00\u5316<\/td>\n0.300<\/td>\n0.04<\/td>\n\u652f\u6301\u5b8c\u6574\uff0c\u53d8\u5316\u660e\u786e<\/td>\n<\/tr>\n
\u8986\u76d6\u4e0d\u5747\u8861<\/td>\n\u22120.167<\/td>\n0.30<\/td>\n\u6781\u4f4e\u8986\u76d6\u6837\u672c<\/td>\n<\/tr>\n
\u5b58\u5728\u7f3a\u5931<\/td>\n0.300<\/td>\n\u672a\u8bbe\u7f6e<\/td>\n\u6709\u6837\u672c\u6ca1\u6709\u4e8b\u4ef6\u652f\u6301<\/td>\n<\/tr>\n
\u53d8\u5316\u5f88\u5c0f<\/td>\n0.010<\/td>\n0.0001<\/td>\n\u663e\u8457\u6807\u7b7e\u4e0d\u4ee3\u8868\u5927\u5e45\u53d8\u5316<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u7ec3\u4e60\u89c4\u5219\u8981\u6c42\u6240\u6709\u91cd\u590d\u6709\u5b9a\u4e49\u7684\u6bd4\u4f8b\u3001\u6bcf\u4efd\u603b\u652f\u6301\u81f3\u5c11\u5341\u3001\u9884\u8bbe FDR \u4e0d\u8d85\u8fc7\u96f6\u70b9\u96f6\u4e94\uff0c\u5e76\u4e14\u5dee\u503c\u7edd\u5bf9\u503c\u81f3\u5c11\u96f6\u70b9\u4e00\u3002\u6700\u7ec8\u53ea\u6709\u7b2c\u4e00\u79cd\u4e8b\u4ef6\u901a\u8fc7\u3002\u8fd9\u91cc\u7684\u6570\u5b57\u7528\u4e8e\u6559\u5b66\uff0c\u4e0d\u662f\u9002\u7528\u4e8e\u6240\u6709\u9879\u76ee\u7684\u901a\u7528\u9608\u503c\u3002<\/p>\n
\u4e0d\u8981\u628a\u67d0\u6761\u7ed3\u679c\u7684 FDR \u76f4\u63a5\u89e3\u91ca\u6210\u8be5\u4e8b\u4ef6\u4e3a\u5047\u7684\u6982\u7387\u3002\u5b83\u6d89\u53ca\u68c0\u9a8c\u96c6\u5408\u4e0e\u591a\u91cd\u6bd4\u8f83\u63a7\u5236\u7684\u542b\u4e49\u3002\u66f4\u4e0d\u80fd\u5728\u6311\u51fa\u611f\u5174\u8da3\u4e8b\u4ef6\u540e\uff0c\u53ea\u5bf9\u8fd9\u4e2a\u5f88\u5c0f\u7684\u5b50\u96c6\u91cd\u65b0\u89e3\u91ca\u539f\u59cb\u7edf\u8ba1\u9608\u503c\uff0c\u800c\u4e0d\u4ea4\u4ee3\u9009\u62e9\u8fc7\u7a0b\u3002<\/p>\n
\u4e0d\u540c\u8ba1\u6570\u53e3\u5f84\u4e0d\u80fd\u76f4\u63a5\u62fc\u6210\u4e00\u5217<\/h2>\n
rMATS-turbo \u63d0\u4f9b\u53ea\u4f7f\u7528\u8fde\u63a5\u652f\u6301\uff0c\u4ee5\u53ca\u540c\u65f6\u4f7f\u7528\u8fde\u63a5\u548c\u5916\u663e\u5b50\u533a\u57df\u652f\u6301\u7684\u7ed3\u679c\u3002\u4e24\u79cd\u53e3\u5f84\u4f1a\u6539\u53d8\u8ba1\u6570\u6765\u6e90\u548c\u76f8\u5e94\u5f52\u4e00\u5316\u6761\u4ef6\u3002\u6bd4\u8f83\u65f6\u5e94\u4fdd\u7559\u6587\u4ef6\u7c7b\u578b\uff0c\u4e0d\u80fd\u628a\u4e00\u4efd\u6837\u672c\u7684\u67d0\u79cd\u53e3\u5f84\u4e0e\u53e6\u4e00\u4efd\u6837\u672c\u7684\u53e6\u4e00\u79cd\u53e3\u5f84\u76f4\u63a5\u62fc\u63a5\u3002<\/p>\n
\u9047\u5230\u540c\u4e00\u4e2a\u5019\u9009\u5728\u4e24\u7c7b\u7ed3\u679c\u4e2d\u8868\u73b0\u4e0d\u540c\uff0c\u53ef\u4ee5\u5148\u68c0\u67e5\u989d\u5916\u533a\u57df\u662f\u5426\u5177\u6709\u8986\u76d6\u652f\u6301\uff0c\u518d\u89c2\u5bdf\u8fde\u63a5\u5c42\u9762\u7684\u8bc1\u636e\u662f\u5426\u4e00\u81f4\u3002\u8fd9\u6837\u7684\u5dee\u5f02\u503c\u5f97\u89e3\u91ca\uff0c\u4f46\u4e0d\u80fd\u7b80\u5355\u628a\u6570\u503c\u66f4\u663e\u8457\u7684\u4e00\u4efd\u6311\u51fa\u6765\u5f53\u6210\u552f\u4e00\u7ed3\u679c\u3002<\/p>\n
\u5b9e\u9645\u8f93\u5165\u8fd8\u53d7\u5230\u8bfb\u957f\u3001\u94fe\u65b9\u5411\u3001\u6bd4\u5bf9\u5904\u7406\u548c\u4e8b\u4ef6\u5b9a\u4e49\u5f71\u54cd\u3002\u653e\u5bbd\u8bfb\u6bb5\u63a5\u7eb3\u6761\u4ef6\u4e0e\u6539\u53d8\u6709\u6548\u957f\u5ea6\u4e0d\u662f\u540c\u4e00\u4ef6\u4e8b\u3002\u89e3\u91ca\u53c2\u6570\u524d\uff0c\u5e94\u5bf9\u7167\u5f53\u524d\u7248\u672c\u7684\u5e2e\u52a9\u548c\u8f93\u51fa\uff0c\u800c\u4e0d\u662f\u628a\u591a\u5e74\u4ee5\u524d\u7684\u547d\u4ee4\u539f\u6837\u5f53\u6210\u6c38\u4e45\u89c4\u5219\u3002<\/p>\n
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\u600e\u6837\u8fd0\u884c\u8fd9\u4efd\u7ec3\u4e60<\/h2>\n
\u9644\u4ef6\u811a\u672c\u53ea\u4f9d\u8d56 Python \u6807\u51c6\u5e93\uff0c\u4f1a\u751f\u6210\u865a\u6784\u8ba1\u6570\u8868\u3001\u8ba1\u7b97\u7ed3\u679c\u53ca\u9010\u9879\u68c0\u67e5\u8bb0\u5f55\u3002\u811a\u672c\u548c\u7ed3\u679c\u5747\u660e\u786e\u6807\u8bb0\u6ca1\u6709\u6267\u884c rMATS\uff0c\u4e0d\u9700\u8981\u76f8\u5e94\u8f6f\u4ef6\u73af\u5883\uff0c\u4e5f\u4e0d\u4f1a\u628a\u624b\u5de5\u8bbe\u7f6e\u7684 FDR \u5305\u88c5\u6210\u7edf\u8ba1\u8f93\u51fa\u3002<\/p>\n
python3 153-rmats-turbo\/run.py results\/rmats-review\r\n<\/code><\/pre>\n
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def psi(inclusion, skipping, inclusion_length, skipping_length):\r\n    assert inclusion >= 0 and skipping >= 0\r\n    assert inclusion_length > 0 and skipping_length > 0\r\n    inc = inclusion \/ inclusion_length\r\n    skip = skipping \/ skipping_length\r\n    return inc \/ (inc + skip) if inc + skip else None\r\n<\/code><\/pre>\n
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