{"id":73152,"date":"2026-11-15T14:14:03","date_gmt":"2026-11-15T06:14:03","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73152"},"modified":"2026-11-15T14:14:03","modified_gmt":"2026-11-15T06:14:03","slug":"leafcutter-junction-filter-cluster-denominator-regtools-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/leafcutter-junction-filter-cluster-denominator-regtools-practice\/","title":{"rendered":"LeafCutter \u5b9e\u8dd1\uff0c\u5220\u6389\u4e00\u6761\u4f4e\u652f\u6301\u8fde\u63a5\uff0c\u4e3a\u4ec0\u4e48\u5176\u4ed6\u5185\u542b\u5b50\u7684\u6bd4\u4f8b\u4e5f\u53d8\u4e86"},"content":{"rendered":"
\n

\u526a\u63a5\u5206\u6790\u4e2d\uff0c\u8fc7\u6ee4\u4f4e\u652f\u6301\u8fde\u63a5\u901a\u5e38\u88ab\u7406\u89e3\u4e3a\u5220\u6389\u51e0\u6761\u4e0d\u53ef\u9760\u8bb0\u5f55\u3002\u4f46\u5982\u679c\u8fd9\u6761\u8fde\u63a5\u6070\u597d\u628a\u4e24\u4e2a\u5c40\u90e8\u7ed3\u6784\u8fde\u5728\u4e00\u8d77\uff0c\u5220\u9664\u5b83\u8fd8\u53ef\u80fd\u62c6\u5f00\u526a\u63a5\u7c07\uff0c\u8fdb\u800c\u6539\u53d8\u5176\u4f59\u5185\u542b\u5b50\u7684\u6bd4\u4f8b\u5206\u6bcd\u3002\u6b64\u65f6\u67d0\u4e2a\u6bd4\u4f8b\u660e\u663e\u4e0a\u5347\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u5bf9\u5e94\u7684\u539f\u59cb\u652f\u6301\u589e\u52a0\u4e86\u3002<\/p>\n

\u672c\u7bc7\u5b9e\u9645\u5b89\u88c5\u7ecf\u5178 LeafCutter \u9879\u76ee\u7684\u805a\u7c7b\u811a\u672c\u53ca RegTools\uff0c\u6784\u9020\u56db\u4efd\u53ef\u624b\u7b97\u7684\u6bd4\u5bf9\u8f93\u5165\uff0c\u8fd0\u884c\u8fde\u63a5\u63d0\u53d6\u548c\u4e09\u7ec4\u805a\u7c7b\u3002\u6211\u4eec\u4ece\u539f\u59cb\u8ba1\u6570\u8ffd\u8e2a\u5230\u7c07\u7ed3\u6784\u4e0e\u5206\u6bcd\u53d8\u5316\uff0c\u89e3\u91ca\u4e3a\u4ec0\u4e48\u8fc7\u6ee4\u89c4\u5219\u4e5f\u662f\u5206\u6790\u5bf9\u8c61\u5b9a\u4e49\u7684\u4e00\u90e8\u5206\u3002<\/p>\n

\"\u5220\u6389\u4e00\u6761\u8fde\u63a5\uff0c\u5206\u6bcd\u4e5f\u4f1a\u6539\u53d8\u3002\u56db\u4efd\u4eba\u5de5\u8f93\u5165\uff0c\u5b9e\u8dd1\u8fde\u63a5\u63d0\u53d6\u4e0e\u4e09\u7ec4\u805a\u7c7b\u30021
\u56db\u4efd\u4eba\u5de5\u8f93\u5165\uff0c\u5b9e\u8dd1\u8fde\u63a5\u63d0\u53d6\u4e0e\u4e09\u7ec4\u805a\u7c7b<\/figcaption><\/figure>\n

\u5148\u786e\u8ba4\u5b89\u88c5\u7684\u662f\u54ea\u4e00\u79cd\u5b9e\u73b0<\/h2>\n

\u8f6f\u4ef6\u540d\u79f0\u76f8\u540c\uff0c\u4e0d\u4ee3\u8868\u5305\u5185\u5bb9\u3001\u5165\u53e3\u548c\u7edf\u8ba1\u5b9e\u73b0\u5b8c\u5168\u76f8\u540c\u3002\u672c\u6b21\u68c0\u67e5\u65f6\uff0cBioconda \u7684\u540c\u540d\u5305\u5df2\u7ecf\u6307\u5411\u65b0\u7684 Python \u9879\u76ee\uff0c\u800c\u7ecf\u5178\u6587\u6863\u4ecd\u4ecb\u7ecd\u805a\u7c7b\u811a\u672c\u53ca\u914d\u5957 R \u5206\u6790\u3002\u5b89\u88c5\u524d\u5e94\u5148\u6838\u5bf9\u5305\u6765\u6e90\uff0c\u4e0d\u80fd\u53ea\u6bd4\u8f83\u540d\u79f0\u3002<\/p>\n

\u672c\u5b9e\u9a8c\u9009\u62e9\u7ecf\u5178\u9879\u76ee\u7684\u4e00\u4e2a\u56fa\u5b9a\u63d0\u4ea4\uff0c\u4e0b\u8f7d\u5e76\u6821\u9a8c\u805a\u7c7b\u811a\u672c\u6458\u8981\uff0c\u5728 Python \u4e09\u70b9\u5341\u70b9\u5341\u4e8c\u73af\u5883\u6267\u884c\u3002\u5b9e\u9645\u6ca1\u6709\u5b89\u88c5\u6216\u6d4b\u8bd5\u65b0\u7684\u540c\u540d\u5b9e\u73b0\uff0c\u4e5f\u6ca1\u6709\u8fd0\u884c\u7ecf\u5178 R \u5dee\u5f02\u526a\u63a5\u6a21\u5757\u3002<\/p>\n

\u5b98\u65b9\u5b89\u88c5\u8bf4\u660e\u5c06\u53ea\u9700\u805a\u7c7b\u8ba1\u6570\u7684\u4f7f\u7528\u573a\u666f\u4e0e\u9700\u8981 R \u6a21\u5757\u7684\u5dee\u5f02\u5206\u6790\u5206\u5f00\u3002\u672c\u7bc7\u5b8c\u6210\u524d\u4e00\u79cd\u5de5\u4f5c\uff0c\u518d\u7528\u624b\u7b97\u89e3\u91ca\u7ed3\u679c\uff0c\u4e0d\u628a\u83b7\u5f97\u8ba1\u6570\u77e9\u9635\u5199\u6210\u5df2\u7ecf\u5b8c\u6210\u663e\u8457\u6027\u68c0\u9a8c\u3002LeafCutter \u5b89\u88c5\u8bf4\u660e<\/a><\/p>\n

\u7ecf\u5178\u9879\u76ee\u63d0\u4ea4\r\n2c9907ef66adf0bfb3092f0ceb6886ee5c046fbb\r\n\r\n\u805a\u7c7b\u811a\u672c SHA256\r\n4228ef4ff41625a0bc3d004cff85bd7a6eb82e44b8f64c021e997077d587293f\r\n\r\nRegTools \u5b9e\u9645\u7248\u672c\r\n1.0.0\r\n<\/code><\/pre>\n
\u8f93\u5165\u5305\u542b\u4e00\u6761\u5bb9\u6613\u88ab\u5ffd\u7565\u7684\u6865\u63a5\u8fde\u63a5<\/h2>\n
\u6211\u4eec\u8bbe\u8ba1\u516d\u79cd\u5185\u542b\u5b50\u533a\u95f4\uff0c\u56db\u4efd\u8f93\u5165\u6bcf\u4efd\u4e00\u767e\u4e8c\u5341\u4e8c\u6761\u6bd4\u5bf9\u8bb0\u5f55\u3002\u5176\u4e2d\u4e94\u79cd\u901a\u8fc7\u5171\u4eab\u8d77\u70b9\u6216\u7ec8\u70b9\u6784\u6210\u76f8\u8fde\u5173\u7cfb\uff0c\u53e6\u4e00\u79cd\u5b64\u7acb\u5b58\u5728\uff0c\u6ca1\u6709\u5176\u4ed6\u5019\u9009\u4e0e\u5b83\u7ec4\u6210\u53ef\u53d8\u9009\u62e9\u3002<\/p>\n
\u524d\u4e24\u4e2a\u5185\u542b\u5b50\u5171\u4eab\u5de6\u7aef\uff0c\u540e\u4e24\u4e2a\u5171\u4eab\u53f3\u7aef\u3002\u4e2d\u95f4\u7684\u4f4e\u652f\u6301\u5185\u542b\u5b50\u540c\u65f6\u8fde\u63a5\u524d\u4e00\u90e8\u5206\u7684\u4e00\u4e2a\u7aef\u70b9\u548c\u540e\u4e00\u90e8\u5206\u7684\u53e6\u4e00\u4e2a\u7aef\u70b9\uff0c\u56e0\u6b64\u628a\u539f\u672c\u53ef\u80fd\u5206\u5f00\u7684\u4e24\u4e2a\u96c6\u5408\u4e32\u4e86\u8d77\u6765\u3002\u8fd9\u91cc\u79f0\u5b83\u4e3a\u6865\u63a5\u8fde\u63a5\uff0c\u53ea\u63cf\u8ff0\u56fe\u4e0a\u7684\u4f5c\u7528\uff0c\u4e0d\u6697\u793a\u5b83\u4e00\u5b9a\u662f\u4f2a\u5f71\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n\n\n
\u8bbe\u8ba1\u5185\u542b\u5b50<\/th>\nS1\u3001S2 \u5404\u81ea\u8ba1\u6570<\/th>\nS3\u3001S4 \u5404\u81ea\u8ba1\u6570<\/th>\n<\/tr>\n<\/thead>\n
A1<\/td>\n30<\/td>\n10<\/td>\n<\/tr>\n
A2<\/td>\n10<\/td>\n30<\/td>\n<\/tr>\n
\u6865\u63a5<\/td>\n2<\/td>\n2<\/td>\n<\/tr>\n
B1<\/td>\n20<\/td>\n20<\/td>\n<\/tr>\n
B2<\/td>\n20<\/td>\n20<\/td>\n<\/tr>\n
\u5b64\u7acb\u5185\u542b\u5b50<\/td>\n40<\/td>\n40<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
A1\u3001A2 \u7684\u4f7f\u7528\u6bd4\u4f8b\u5728\u524d\u540e\u4e24\u5bf9\u8f93\u5165\u4e2d\u4e0d\u540c\uff0cB1\u3001B2 \u5219\u4fdd\u6301\u76f8\u7b49\u3002\u6865\u63a5\u8fde\u63a5\u6bcf\u4efd\u53ea\u6709\u4e24\u6761\uff0c\u4f46\u5408\u5e76\u56db\u4efd\u4ee5\u540e\u4e3a\u516b\u6761\u3002\u5b64\u7acb\u5185\u542b\u5b50\u7684\u652f\u6301\u5f88\u9ad8\uff0c\u7528\u6765\u68c0\u67e5\u4e0d\u8fdb\u5165\u53ef\u53d8\u7c07\u662f\u5426\u4e00\u5b9a\u610f\u5473\u7740\u8986\u76d6\u4e0d\u8db3\u3002<\/p>\n
\u8fd9\u4e9b\u8f93\u5165\u4e0d\u662f\u751f\u7269\u5b66\u91cd\u590d\uff0c\u4e5f\u6ca1\u6709\u771f\u5b9e\u6d4b\u5e8f\u8fc7\u7a0b\u3002SAM \u7684 CIGAR\u3001\u94fe\u6807\u7b7e\u548c\u652f\u6301\u6761\u6570\u7531\u6211\u4eec\u660e\u786e\u5199\u5165\uff0c\u5e8f\u5217\u4f7f\u7528\u683c\u5f0f\u6d4b\u8bd5\u5360\u4f4d\u503c\u3002\u5b9e\u9645\u8fd0\u884c\u7684\u662f\u8fd9\u4e9b\u7ed3\u6784\u8bb0\u5f55\u7684\u63d0\u53d6\u4e0e\u805a\u7c7b\uff0c\u4e0d\u5305\u542b\u4e0a\u6e38\u6bd4\u5bf9\u51c6\u786e\u7387\u8bc4\u4f30\u3002<\/p>\n
\u8fde\u63a5\u63d0\u53d6\u5b9e\u9645\u6267\u884c\u4e86\u54ea\u4e9b\u6761\u4ef6<\/h2>\n
\u6bcf\u6761\u8bbe\u8ba1\u8bb0\u5f55\u957f\u5ea6\u4e00\u767e\uff0c\u8de8\u4e00\u4e2a\u5185\u542b\u5b50\uff0c\u4e24\u4fa7\u5404\u4e94\u5341\u78b1\u57fa\u3002\u6211\u4eec\u7528 Samtools \u5b8c\u6210\u6392\u5e8f\u548c\u7d22\u5f15\uff0c\u518d\u6267\u884c RegTools \u63d0\u53d6\u3002\u94fe\u65b9\u5411\u6765\u81ea\u8f93\u5165\u4e2d\u660e\u786e\u8bbe\u7f6e\u7684\u6807\u7b7e\uff0c\u6240\u6709\u8bbe\u8ba1\u8fde\u63a5\u90fd\u5728\u6240\u8bbe\u957f\u5ea6\u8303\u56f4\u5185\u3002<\/p>\n
.\/regtools junctions extract \\\r\n  -a 8 -m 50 -M 500000 -s XS \\\r\n  -o S1.junc S1.bam\r\n<\/code><\/pre>\n
\u56db\u4efd\u8f93\u5165\u5404\u63d0\u53d6\u516d\u6761\u8fde\u63a5\u8bb0\u5f55\uff0c\u8ba1\u6570\u4e0e\u8bbe\u8ba1\u4e00\u81f4\u3002\u8f93\u51fa\u662f\u5305\u542b\u4e24\u4fa7\u951a\u5b9a\u533a\u6bb5\u7684 BED12\uff0c\u4e0d\u80fd\u53ea\u62ff\u5916\u5c42\u8d77\u6b62\u5750\u6807\u5f53\u4f5c\u5185\u542b\u5b50\u8fb9\u754c\uff0c\u9700\u8981\u7ed3\u5408\u533a\u6bb5\u957f\u5ea6\u3002<\/p>\n
\u5b89\u88c5\u65f6\uff0c\u5bb9\u5668\u955c\u50cf\u62c9\u53d6\u5904\u4e8e\u6392\u961f\u72b6\u6001\u3002\u6211\u4eec\u6539\u7528\u6821\u9a8c\u8fc7\u6458\u8981\u7684 Bioconda \u4e8c\u8fdb\u5236\u5305\uff0c\u5728\u56fa\u5b9a\u8f85\u52a9\u5bb9\u5668\u4e2d\u8fd0\u884c\uff0c\u5e76\u663e\u5f0f\u6307\u5b9a\u5df2\u6709\u5e93\u8def\u5f84\u3002\u4e3b\u7a0b\u5e8f\u7248\u672c\u68c0\u67e5\u548c\u56db\u6b21\u63d0\u53d6\u5747\u6210\u529f\uff0c\u5b8c\u6574\u9a71\u52a8\u5df2\u7ecf\u5305\u542b\u8fd9\u9879\u5b89\u88c5\u65b9\u5f0f\u3002<\/p>\n
\u8fd9\u4e2a\u64cd\u4f5c\u53ea\u5f71\u54cd\u72ec\u7acb\u5b9e\u9a8c\u76ee\u5f55\uff0c\u6ca1\u6709\u6539\u5199\u7cfb\u7edf\u73af\u5883\u3002\u8bfb\u8005\u91cd\u8dd1\u65f6\u76f4\u63a5\u6267\u884c\u9a71\u52a8\u5373\u53ef\uff0c\u5de5\u5177\u5305\u6458\u8981\u3001\u4e8c\u8fdb\u5236\u6458\u8981\u548c\u8fd0\u884c\u73af\u5883\u90fd\u5728\u7ed3\u679c\u8bb0\u5f55\u4e2d\uff0c\u4fbf\u4e8e\u68c0\u67e5\u5b9e\u9645\u4f7f\u7528\u7684\u7a0b\u5e8f\u662f\u5426\u4e00\u81f4\u3002<\/p>\n
RegTools \u7684\u951a\u5b9a\u957f\u5ea6\u3001\u94fe\u6765\u6e90\u4e0e\u5185\u542b\u5b50\u957f\u5ea6\u5206\u522b\u63a7\u5236\u4e0d\u540c\u6761\u4ef6\uff0c\u4e0d\u80fd\u628a\u5176\u4e2d\u4e00\u4e2a\u53c2\u6570\u5f53\u6210\u603b\u4f53\u8d28\u91cf\u5206\u6570\u3002\u672c\u6b21\u56fa\u5b9a\u8fd9\u4e9b\u6761\u4ef6\uff0c\u53ea\u6bd4\u8f83\u540e\u7eed\u805a\u7c7b\u89c4\u5219\u3002RegTools \u8fde\u63a5\u63d0\u53d6\u8bf4\u660e<\/a><\/p>\n
\u4e09\u7ec4\u805a\u7c7b\u53ea\u6539\u53d8\u4e24\u4e2a\u7b5b\u9009\u6761\u4ef6<\/h2>\n
\u7b2c\u4e00\u7ec4\u4f7f\u7528\u8f83\u4f4e\u7684\u6bd4\u4f8b\u95e8\u69db\u548c\u652f\u6301\u95e8\u69db\u3002\u7b2c\u4e8c\u7ec4\u53ea\u63d0\u9ad8\u6bd4\u4f8b\u95e8\u69db\uff0c\u7b2c\u4e09\u7ec4\u5219\u4fdd\u6301\u4f4e\u6bd4\u4f8b\u95e8\u69db\u3001\u63d0\u9ad8\u652f\u6301\u6570\u91cf\u95e8\u69db\u3002\u8f93\u5165\u8fde\u63a5\u6587\u4ef6\u53ca\u5176\u987a\u5e8f\u59cb\u7ec8\u76f8\u540c\u3002<\/p>\n
python3 leafcutter_cluster_regtools.py \\\r\n  -j junctions.txt -o toy -r permissive \\\r\n  -l 500000 -p 0.001 -m 3\r\n\r\npython3 leafcutter_cluster_regtools.py \\\r\n  -j junctions.txt -o toy -r ratio_filter \\\r\n  -l 500000 -p 0.05 -m 3\r\n\r\npython3 leafcutter_cluster_regtools.py \\\r\n  -j junctions.txt -o toy -r support_filter \\\r\n  -l 500000 -p 0.001 -m 10\r\n<\/code><\/pre>\n
\u8fd9\u4e9b\u6570\u503c\u7528\u4e8e\u653e\u5927\u4e00\u4e2a\u53ef\u4ee5\u89e3\u91ca\u7684\u53d8\u5316\uff0c\u4e0d\u662f\u5927\u89c4\u6a21\u961f\u5217\u63a8\u8350\u914d\u7f6e\u3002\u4e09\u4e2a\u8f93\u51fa\u76ee\u5f55\u5206\u522b\u4fdd\u5b58\u6c47\u603b\u5019\u9009\u3001\u7cbe\u70bc\u540e\u7684\u7c07\u3001\u6574\u6570\u8ba1\u6570\u548c\u5e26\u5206\u6bcd\u7684\u8ba1\u6570\u6587\u4ef6\uff0c\u65b9\u4fbf\u9010\u6b65\u5b9a\u4f4d\u5dee\u5f02\u3002<\/p>\n
\u4e09\u7ec4\u5b9e\u9645\u8fd0\u884c\u90fd\u6b63\u5e38\u9000\u51fa\u3002\u7b2c\u4e00\u7ec4\u5f97\u5230\u4e00\u4e2a\u542b\u4e94\u4e2a\u5185\u542b\u5b50\u7684\u7c07\uff0c\u540e\u4e24\u7ec4\u5747\u5f97\u5230\u4e24\u4e2a\u7c07\uff0c\u6bcf\u4e2a\u5305\u542b\u4e24\u4e2a\u5185\u542b\u5b50\u3002\u5b64\u7acb\u5185\u542b\u5b50\u6ca1\u6709\u8fdb\u5165\u8fd9\u4e9b\u6700\u7ec8\u53ef\u53d8\u7c07\u3002<\/p>\n
\u7b2c\u4e00\u7ec4\uff0c\u4e00\u4e2a\u7c07\u91cc\u7684\u5206\u6bcd\u4e3a\u4ec0\u4e48\u662f\u516b\u5341\u4e8c<\/h2>\n
\u4f4e\u95e8\u69db\u7ec4\u4fdd\u7559\u6865\u63a5\u8fde\u63a5\uff0c\u4e94\u79cd\u76f8\u8fde\u5185\u542b\u5b50\u7ec4\u6210\u540c\u4e00\u4e2a\u7c07\u3002\u6bcf\u4efd\u8f93\u5165\u5728\u8fd9\u4e2a\u7c07\u4e2d\u7684\u8ba1\u6570\u548c\u4e3a\u516b\u5341\u4e8c\uff0c\u6765\u81ea\u56db\u5341\u6761 A \u90e8\u5206\u3001\u56db\u5341\u6761 B \u90e8\u5206\u548c\u4e24\u6761\u6865\u63a5\u652f\u6301\u3002<\/p>\n
\u56e0\u6b64\uff0c\u7b2c\u4e00\u4efd\u8f93\u5165\u7684 A1 \u8bb0\u5f55\u4e3a\u4e09\u5341\u9664\u4ee5\u516b\u5341\u4e8c\uff0cA2 \u4e3a\u5341\u9664\u4ee5\u516b\u5341\u4e8c\uff0cB1 \u4e0e B2 \u5404\u4e3a\u4e8c\u5341\u9664\u4ee5\u516b\u5341\u4e8c\u3002\u7b2c\u4e09\u4efd\u8f93\u5165\u7684 A1\u3001A2 \u5bf9\u8c03\uff0c\u4f46\u5206\u6bcd\u4ecd\u4e3a\u516b\u5341\u4e8c\u3002<\/p>\n
\u4f4e\u95e8\u69db\u7ec4 S1\r\nA1        30\/82\r\nA2        10\/82\r\n\u6865\u63a5       2\/82\r\nB1        20\/82\r\nB2        20\/82\r\n<\/code><\/pre>\n
\u8fd9\u4e2a\u5206\u6bcd\u4e0d\u662f\u6837\u672c\u603b\u8bfb\u6bb5\u6570\u3002\u4e00\u767e\u4e8c\u5341\u4e8c\u6761\u8f93\u5165\u4e2d\u7684\u56db\u5341\u6761\u5b64\u7acb\u5185\u542b\u5b50\u652f\u6301\u6ca1\u6709\u8fdb\u5165\u8be5\u7c07\uff0c\u56e0\u6b64\u4e0d\u5728\u5206\u6bcd\u4e2d\u3002\u5b83\u4e5f\u4e0d\u662f\u6574\u4e2a\u57fa\u56e0\u7684\u8f6c\u5f55\u672c\u6570\uff0c\u56e0\u4e3a\u5b9e\u9a8c\u6839\u672c\u6ca1\u6709\u63d0\u4f9b\u57fa\u56e0\u6ce8\u91ca\u3002<\/p>\n
\u6bd4\u4f8b\u89e3\u91ca\u5fc5\u987b\u5e26\u7740\u5b83\u6240\u5c5e\u7684\u96c6\u5408\u3002\u53ea\u5199 A1 \u4f7f\u7528\u6bd4\u4f8b\u7ea6\u4e3a\u767e\u5206\u4e4b\u4e09\u5341\u516d\u70b9\u516d\uff0c\u5374\u7701\u7565\u7c07\u5b9a\u4e49\uff0c\u8bfb\u8005\u5c31\u65e0\u6cd5\u5224\u65ad\u8fd9\u4e2a\u6570\u503c\u4e0e\u53e6\u4e00\u5957\u5206\u6790\u662f\u5426\u5728\u6bd4\u8f83\u540c\u4e00\u4ef6\u4e8b\u3002<\/p>\n
\u63d0\u9ad8\u6bd4\u4f8b\u95e8\u69db\uff0c\u5220\u9664\u7684\u8fde\u63a5\u8fd8\u6539\u53d8\u4e86\u7c07\u7ed3\u6784<\/h2>\n
\u5408\u5e76\u56db\u4efd\u8f93\u5165\u540e\uff0c\u6865\u63a5\u8fde\u63a5\u603b\u652f\u6301\u4e3a\u516b\uff0c\u5176\u4ed6\u56db\u79cd\u76f8\u8fde\u5185\u542b\u5b50\u5404\u4e3a\u516b\u5341\uff0c\u603b\u8ba1\u4e09\u767e\u4e8c\u5341\u516b\u3002\u6865\u63a5\u5360\u6bd4\u7ea6\u4e3a\u767e\u5206\u4e4b\u4e8c\u70b9\u56db\u56db\uff0c\u8d85\u8fc7\u7b2c\u4e00\u7ec4\u7684\u4f4e\u95e8\u69db\uff0c\u4f46\u4f4e\u4e8e\u7b2c\u4e8c\u7ec4\u7684\u767e\u5206\u4e4b\u4e94\u3002<\/p>\n
\u6865\u63a5\u88ab\u8fc7\u6ee4\u540e\uff0c\u524d\u4e00\u90e8\u5206\u4e0e\u540e\u4e00\u90e8\u5206\u4e0d\u518d\u901a\u8fc7\u5171\u4eab\u7aef\u70b9\u76f8\u8fde\uff0c\u4e8e\u662f\u7cbe\u70bc\u8fc7\u7a0b\u628a\u5b83\u4eec\u62c6\u6210\u4e24\u4e2a\u7c07\u3002A1 \u4e0e A2 \u4e00\u7ec4\uff0cB1 \u4e0e B2 \u53e6\u4e00\u7ec4\u3002\u539f\u6765\u7684\u56db\u79cd\u4e3b\u8981\u5185\u542b\u5b50\u8ba1\u6570\u90fd\u6ca1\u6709\u6539\u53d8\u3002<\/p>\n
\u8fd9\u65f6\uff0c\u7b2c\u4e00\u4efd\u8f93\u5165\u7684 A1 \u53d8\u6210\u4e09\u5341\u9664\u4ee5\u56db\u5341\uff0c\u4e5f\u5c31\u662f\u96f6\u70b9\u4e03\u4e94\uff0c\u539f\u5148\u7ea6\u4e3a\u96f6\u70b9\u4e09\u516d\u516d\u3002\u770b\u8d77\u6765\u589e\u957f\u4e86\u5f88\u591a\uff0c\u4f46\u5206\u5b50\u4e00\u76f4\u90fd\u662f\u4e09\u5341\uff0c\u53d8\u5316\u5168\u90e8\u6765\u81ea\u5206\u6bcd\u4ece\u516b\u5341\u4e8c\u53d8\u6210\u56db\u5341\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
S1 \u4e2d\u7684\u5bf9\u8c61<\/th>\n\u4f4e\u95e8\u69db\u7ec4<\/th>\n\u8fc7\u6ee4\u6865\u63a5\u540e<\/th>\n<\/tr>\n<\/thead>\n
A1 \u539f\u59cb\u8ba1\u6570<\/td>\n30<\/td>\n30<\/td>\n<\/tr>\n
A1 \u7684\u6bd4\u4f8b<\/td>\n30\/82<\/td>\n30\/40<\/td>\n<\/tr>\n
B1 \u539f\u59cb\u8ba1\u6570<\/td>\n20<\/td>\n20<\/td>\n<\/tr>\n
B1 \u7684\u6bd4\u4f8b<\/td>\n20\/82<\/td>\n20\/40<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u5982\u679c\u628a\u4e24\u6b21\u8fd0\u884c\u5f53\u6210\u4e24\u4e2a\u751f\u7269\u5b66\u6761\u4ef6\uff0c\u76f4\u63a5\u6bd4\u8f83\u6bd4\u4f8b\uff0c\u5c31\u4f1a\u5f97\u5230\u4e00\u4e2a\u4eba\u4e3a\u5236\u9020\u7684\u53d8\u5316\u3002\u53c2\u6570\u654f\u611f\u6027\u68c0\u67e5\u5e94\u8be5\u89e3\u91ca\u5206\u6790\u96c6\u5408\u5982\u4f55\u6539\u53d8\uff0c\u4e0d\u80fd\u628a\u53c2\u6570\u9020\u6210\u7684\u5206\u6bcd\u53d8\u5316\u5305\u88c5\u6210\u8868\u8fbe\u54cd\u5e94\u3002<\/p>\n
\u652f\u6301\u6570\u91cf\u95e8\u69db\u4e5f\u53ef\u80fd\u5728\u7c07\u5185\u90e8\u8d77\u4f5c\u7528<\/h2>\n
\u7b2c\u4e09\u7ec4\u4fdd\u6301\u4f4e\u6bd4\u4f8b\u95e8\u69db\uff0c\u628a\u652f\u6301\u6570\u91cf\u6761\u4ef6\u4ece\u4e09\u6539\u4e3a\u5341\u3002\u6865\u63a5\u8fde\u63a5\u5408\u5e76\u540e\u53ea\u6709\u516b\u6761\uff0c\u56e0\u6b64\u4e5f\u88ab\u53bb\u6389\uff0c\u5f97\u5230\u4e0e\u7b2c\u4e8c\u7ec4\u76f8\u540c\u7684\u4e24\u4e2a\u7c07\u3002<\/p>\n
\u8fd9\u4e00\u7ed3\u679c\u63d0\u9192\u6211\u4eec\uff0c\u53c2\u6570\u540d\u79f0\u6216\u5e2e\u52a9\u4e2d\u7684\u7b80\u77ed\u63cf\u8ff0\u4e0d\u4e00\u5b9a\u8db3\u4ee5\u8bf4\u660e\u5168\u90e8\u4f5c\u7528\u4f4d\u7f6e\u3002\u5728\u672c\u6b21\u56fa\u5b9a\u811a\u672c\u91cc\uff0c\u8be5\u6570\u91cf\u95e8\u69db\u65e2\u53c2\u4e0e\u5019\u9009\u7c07\u7b5b\u9009\uff0c\u4e5f\u88ab\u4f20\u5165\u5185\u90e8\u8fde\u63a5\u7cbe\u70bc\u3002\u539f\u59cb\u7c07\u603b\u652f\u6301\u8fdc\u9ad8\u4e8e\u5341\uff0c\u5e76\u4e0d\u80fd\u4fdd\u8bc1\u7c07\u5185\u6bcf\u6761\u8fde\u63a5\u90fd\u88ab\u4fdd\u7559\u3002<\/p>\n
\u6211\u4eec\u540c\u65f6\u6838\u5bf9\u4e86\u5b9e\u9645\u8f93\u51fa\u548c\u56fa\u5b9a\u7248\u672c\u6e90\u7801\uff0c\u786e\u8ba4\u7b2c\u4e09\u7ec4\u7684\u53d8\u5316\u53d1\u751f\u5728\u8fde\u63a5\u7b5b\u9009\u4e4b\u540e\u3002\u8bfb\u8005\u82e5\u9047\u5230\u603b\u652f\u6301\u5f88\u9ad8\u5374\u6709\u8fde\u63a5\u6d88\u5931\uff0c\u5e94\u68c0\u67e5\u95e8\u69db\u662f\u5426\u8fd8\u4f5c\u7528\u4e8e\u5355\u6761\u8fde\u63a5\uff0c\u800c\u4e0d\u662f\u76f4\u63a5\u5224\u65ad\u7a0b\u5e8f\u4e22\u6570\u636e\u3002\u672c\u6b21\u4f7f\u7528\u7684\u7ecf\u5178\u805a\u7c7b\u811a\u672c<\/a><\/p>\n
\u8fd9\u4e5f\u8bf4\u660e\uff0c\u4e0d\u80fd\u53ea\u8bb0\u5f55\u53c2\u6570\u503c\u800c\u4e0d\u8bb0\u5f55\u7248\u672c\u3002\u540c\u4e00\u4e2a\u9009\u9879\u5728\u4e0d\u540c\u5b9e\u73b0\u4e2d\u7684\u4f5c\u7528\u8303\u56f4\uff0c\u53ef\u80fd\u9700\u8981\u91cd\u65b0\u6838\u5bf9\u3002\u672c\u7bc7\u7ed3\u8bba\u9650\u5b9a\u4e8e\u6240\u5217\u63d0\u4ea4\u548c\u5b9e\u9645\u8fd0\u884c\uff0c\u4e0d\u5ef6\u4f38\u4e3a\u6240\u6709\u540c\u540d\u5305\u7684\u7edf\u4e00\u884c\u4e3a\u3002<\/p>\n
\u9ad8\u652f\u6301\u5b64\u7acb\u5185\u542b\u5b50\u6ca1\u6709\u8f93\u51fa\uff0c\u4e0d\u7b49\u4e8e\u6ca1\u6709\u8868\u8fbe<\/h2>\n
\u5b64\u7acb\u5185\u542b\u5b50\u6bcf\u4efd\u6709\u56db\u5341\u6761\u652f\u6301\uff0c\u5408\u8ba1\u4e00\u767e\u516d\u5341\uff0c\u6bd4\u6865\u63a5\u8fde\u63a5\u9ad8\u5f97\u591a\u3002RegTools \u786e\u5b9e\u63d0\u53d6\u5230\u4e86\u5b83\uff0c\u4f46\u672c\u6b21\u9ed8\u8ba4\u53ef\u53d8\u805a\u7c7b\u8f93\u51fa\u6ca1\u6709\u4fdd\u7559\u5b83\u3002<\/p>\n
\u539f\u56e0\u5728\u4e8e\u5b83\u6ca1\u6709\u5f62\u6210\u9700\u8981\u6bd4\u8f83\u7684\u591a\u5206\u652f\u96c6\u5408\u3002\u6700\u7ec8\u77e9\u9635\u7684\u76ee\u7684\u4e0d\u662f\u5217\u51fa\u6240\u6709\u89c2\u6d4b\u5230\u7684\u8fde\u63a5\uff0c\u66f4\u4e0d\u662f\u63d0\u4f9b\u5168\u90e8\u57fa\u56e0\u7684\u8868\u8fbe\u8ba1\u6570\u3002\u56e0\u6b64\uff0c\u6700\u7ec8\u6587\u4ef6\u4e2d\u4e0d\u5b58\u5728\u67d0\u6761\u5185\u542b\u5b50\uff0c\u4e0d\u80fd\u53cd\u63a8\u539f\u59cb\u6570\u636e\u6ca1\u6709\u652f\u6301\u3002<\/p>\n
\u5b9a\u4f4d\u7f3a\u5931\u9879\u65f6\uff0c\u5e94\u5148\u68c0\u67e5\u8fde\u63a5\u63d0\u53d6\u6587\u4ef6\uff0c\u518d\u770b\u6c47\u603b\u5019\u9009\u548c\u7cbe\u70bc\u7ed3\u679c\u3002\u8fd9\u6837\u53ef\u4ee5\u533a\u5206\u6ca1\u6709\u88ab\u63d0\u53d6\u3001\u6ca1\u6709\u8fdb\u5165\u5019\u9009\u7c07\u3001\u88ab\u95e8\u69db\u8fc7\u6ee4\u4ee5\u53ca\u6ca1\u6709\u6784\u6210\u53ef\u53d8\u96c6\u5408\u7b49\u4e0d\u540c\u60c5\u51b5\u3002<\/p>\n
\u53ea\u4fdd\u7559\u6700\u7ec8\u77e9\u9635\u800c\u5220\u9664\u4e2d\u95f4\u7ed3\u6784\uff0c\u4f1a\u8ba9\u8fd9\u4e9b\u539f\u56e0\u6df7\u5728\u4e00\u8d77\u3002\u5c24\u5176\u5728\u6ce8\u91ca\u4e4b\u5916\u7684\u5019\u9009\u5206\u6790\u4e2d\uff0c\u80fd\u591f\u8ffd\u8e2a\u4e00\u6761\u8fde\u63a5\u5728\u54ea\u4e00\u6b65\u6d88\u5931\uff0c\u5f80\u5f80\u6bd4\u53ea\u77e5\u9053\u6700\u7ec8\u5269\u4e0b\u591a\u5c11\u884c\u66f4\u6709\u7528\u3002<\/p>\n
\u5750\u6807\u8f6c\u6362\u9700\u8981\u770b\u5b9e\u9645\u5b9a\u4e49<\/h2>\n
\u672c\u5b9e\u9a8c A1 \u7684\u5185\u542b\u5b50\u6309\u96f6\u57fa\u5236\u534a\u5f00\u533a\u95f4\u5199\u4f5c\u4e00\u5343\u5230\u4e8c\u5343\u3002RegTools \u7684\u5916\u5c42\u533a\u95f4\u8fd8\u5305\u542b\u4e24\u4fa7\u5404\u4e94\u5341\u78b1\u57fa\u7684\u951a\u5b9a\u90e8\u5206\uff0c\u7ecf\u5178\u805a\u7c7b\u811a\u672c\u8fdb\u4e00\u6b65\u8f6c\u6362\u540e\uff0c\u8f93\u51fa\u6807\u8bc6\u4e2d\u7684\u53f3\u7aef\u4e3a\u4e8c\u5343\u96f6\u4e00\u3002<\/p>\n
\u8fd9\u4e2a\u5dee\u4e00\u78b1\u57fa\u4e0d\u662f\u968f\u610f\u504f\u79fb\u3002\u5bf9\u5e94\u7684\u8fde\u63a5\u53ef\u4ee5\u7406\u89e3\u4e3a\u5de6\u4fa7\u5916\u663e\u5b50\u672b\u7aef\u5230\u53f3\u4fa7\u5916\u663e\u5b50\u8d77\u59cb\uff0c\u8f93\u51fa\u7aef\u70b9\u5b9a\u4e49\u4e0e\u539f\u59cb\u5185\u542b\u5b50\u534a\u5f00\u533a\u95f4\u4e0d\u540c\u3002\u628a\u5b83\u76f4\u63a5\u5f53\u6210\u666e\u901a BED \u533a\u95f4\u4f7f\u7528\uff0c\u5c31\u53ef\u80fd\u5339\u914d\u4e0d\u5230\u6b63\u786e\u4f4d\u7f6e\u3002<\/p>\n
\u5728\u672c\u6b21\u811a\u672c\u4e2d\uff0c\u5de6\u7aef\u7531\u5916\u5c42\u8d77\u70b9\u52a0\u5de6\u533a\u6bb5\u957f\u5ea6\u5f97\u5230\uff0c\u53f3\u7aef\u7531\u5916\u5c42\u7ec8\u70b9\u51cf\u53f3\u533a\u6bb5\u957f\u5ea6\u518d\u52a0\u4e00\u5f97\u5230\u3002\u5b9e\u9645\u6574\u7406\u6ce8\u91ca\u65f6\uff0c\u5e94\u628a\u8fd9\u4e2a\u8f6c\u6362\u5199\u8fdb\u4ee3\u7801\u4e0e\u8bf4\u660e\uff0c\u800c\u4e0d\u662f\u51ed\u89c2\u5bdf\u4e34\u65f6\u7ed9\u6240\u6709\u5750\u6807\u52a0\u4e00\u6216\u51cf\u4e00\u3002<\/p>\n
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python3 155-leafcutter\/run.py results\/leafcutter\r\n<\/code><\/pre>\n
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