{"id":73171,"date":"2026-11-15T14:33:34","date_gmt":"2026-11-15T06:33:34","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73171"},"modified":"2026-11-15T14:35:24","modified_gmt":"2026-11-15T06:35:24","slug":"arriba-fusion-duplicate-support-coverage-filter-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/arriba-fusion-duplicate-support-coverage-filter-practice\/","title":{"rendered":"Arriba \u5b9e\u8dd1\u590d\u76d8\uff0c\u4ece 40 \u5bf9\u91cd\u590d\u8bfb\u6bb5\u770b\u61c2\u878d\u5408\u652f\u6301\u6570\u3001\u8986\u76d6\u5ea6\u4e0e\u8fc7\u6ee4\u8bb0\u5f55"},"content":{"rendered":"<div class=\"ysx-method-guide\" style=\"max-width:820px;min-width:0;margin:0 auto;color:#26374a;line-height:1.86;overflow-wrap:anywhere\">\n<p>\u4e00\u5f20\u878d\u5408\u7ed3\u679c\u8868\u91cc\uff0c\u652f\u6301\u6570\u589e\u52a0\u901a\u5e38\u8ba9\u4eba\u66f4\u6709\u4fe1\u5fc3\u3002\u4f46\u589e\u52a0\u7684\u7a76\u7adf\u662f\u65b0\u7684\u72ec\u7acb\u7247\u6bb5\uff0c\u8fd8\u662f\u539f\u6709\u7247\u6bb5\u7684\u91cd\u590d\u526f\u672c\uff0c\u5fc5\u987b\u5206\u6e05\u3002\u672c\u6b21\u5728\u540c\u4e00\u6bb5\u4eba\u5de5\u878d\u5408\u5e8f\u5217\u4e0a\u589e\u52a0\u56db\u5341\u5bf9\u91cd\u590d\u8bfb\u6bb5\uff0c\u5b9e\u9645\u8fd0\u884c\u6bd4\u5bf9\u548c\u878d\u5408\u68c0\u6d4b\u540e\uff0c\u6709\u6548\u652f\u6301\u6570\u6ca1\u6709\u589e\u52a0\uff0c\u65ad\u70b9\u9644\u8fd1\u7684\u4e00\u4fa7\u8986\u76d6\u5ea6\u5374\u660e\u663e\u5347\u9ad8\u3002\u5982\u679c\u53ea\u6839\u636e\u8986\u76d6\u6df1\u5ea6\u6392\u5e8f\uff0c\u5c31\u4f1a\u628a\u8fd9\u6b21\u4eba\u4e3a\u91cd\u590d\u5f53\u6210\u66f4\u5f3a\u7684\u8bc1\u636e\u3002<\/p>\n<p>\u8fd9\u7bc7\u6587\u7ae0\u7528\u4e00\u4e2a\u53ef\u4ee5\u5b8c\u6574\u91cd\u5efa\u7684\u5c0f\u5b9e\u9a8c\uff0c\u8bf4\u660e\u5982\u4f55\u4ece\u539f\u59cb\u8bfb\u6bb5\u8ffd\u5230\u6bd4\u5bf9\u65e5\u5fd7\uff0c\u518d\u8ffd\u5230\u878d\u5408\u7ed3\u679c\u4e2d\u7684\u8ba1\u6570\u4e0e\u8fc7\u6ee4\u539f\u56e0\u3002\u5b83\u89e3\u51b3\u7684\u662f\u7ed3\u679c\u5ba1\u9605\u95ee\u9898\u3002\u5b9e\u9a8c\u91cc\u7684\u57fa\u56e0\u3001\u53c2\u8003\u5e8f\u5217\u548c\u8bfb\u6bb5\u5168\u90e8\u7531\u811a\u672c\u751f\u6210\uff0c\u4e0d\u6d89\u53ca\u771f\u5b9e\u6837\u672c\uff0c\u4e5f\u4e0d\u80fd\u636e\u6b64\u4f30\u7b97\u771f\u5b9e\u9879\u76ee\u7684\u68c0\u51fa\u7387\u6216\u5047\u9633\u6027\u7387\u3002<\/p>\n<figure class=\"guide-figure\" style=\"margin:24px 0 28px\"><img loading=\"lazy\" decoding=\"async\" src=\"\/wordpress\/wp-content\/uploads\/2026\/11\/arriba-fusion-duplicate-support-coverage-filter-practice-guide.png\" alt=\"\u91cd\u590d\u591a\u4e86\uff0c\u8bc1\u636e\u672a\u5fc5\u66f4\u5f3a\u300240 \u5bf9\u526f\u672c\u8ba9\u8986\u76d6\u5347\u9ad8\uff0c\u6709\u6548\u652f\u6301\u4ecd\u4e3a 37\u300237 \u2192 37\uff0c\u4fdd\u7559\u91cd\u590d\u8fc7\u6ee4\uff0c\u65b0\u589e\u526f\u672c\u88ab\u8bb0\u5f55\u4e3a duplicates(40)\u300254 \u2192 94\uff0c\u4e59\u4fa7\u8986\u76d6\u5ea6\u5347\u9ad8\uff0c\u8986\u76d6\u5ea6\u4e0e\u6709\u6548\u652f\u6301\u91c7\u7528\u4e0d\u540c\u53e3\u5f84\u300237 \u2192 77\uff0c\u5173\u95ed\u91cd\u590d\u8fc7\u6ee4\uff0c\u652f\u6301\u589e\u52a0\u4e0d\u7b49\u4e8e\u51fa\u73b0\u65b0\u7684\u72ec\u7acb\u7247\u6bb5\u3002\" width=\"1200\" height=\"840\" style=\"display:block;width:100%;height:auto;border-radius:10px\"><figcaption style=\"font-size:14px;line-height:1.6;margin:9px 2px 0;color:#475569\">40 \u5bf9\u526f\u672c\u8ba9\u8986\u76d6\u5347\u9ad8\uff0c\u6709\u6548\u652f\u6301\u4ecd\u4e3a 37<\/figcaption><\/figure>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5148\u660e\u786e Arriba \u63a5\u6536\u7684\u8bc1\u636e<\/h2>\n<p>Arriba \u4ece\u6bd4\u5bf9\u7ed3\u679c\u4e2d\u5bfb\u627e\u5f02\u5e38\u8fde\u63a5\u3002\u771f\u6b63\u8de8\u8d8a\u8fde\u63a5\u4f4d\u7f6e\u7684\u5206\u88c2\u6bd4\u5bf9\uff0c\u548c\u4e00\u5bf9\u8bfb\u6bb5\u5206\u522b\u843d\u5728\u4e24\u4e2a\u533a\u57df\u7684\u8de8\u63a5\u8bc1\u636e\uff0c\u63d0\u4f9b\u7684\u4fe1\u606f\u7cbe\u5ea6\u4e0d\u540c\u3002\u524d\u8005\u80fd\u5e2e\u52a9\u5b9a\u4f4d\u8fde\u63a5\u8fb9\u754c\uff0c\u540e\u8005\u63d0\u4f9b\u7247\u6bb5\u5c42\u9762\u7684\u7a7a\u95f4\u7ea6\u675f\u3002\u5ba1\u9605\u65f6\u5e94\u5206\u522b\u68c0\u67e5\uff0c\u4e0d\u5b9c\u628a\u6240\u6709\u652f\u6301\u538b\u6210\u4e00\u4e2a\u6570\u5b57\u540e\u5c31\u505c\u6b62\u8ffd\u8e2a\u3002<\/p>\n<p>\u5c24\u5176\u8981\u786e\u8ba4\u4f20\u7ed9\u7a0b\u5e8f\u7684\u6bd4\u5bf9\u6587\u4ef6\u786e\u5b9e\u542b\u6709\u5d4c\u5408\u6bd4\u5bf9\u3002\u666e\u901a\u8868\u8fbe\u5b9a\u91cf\u6d41\u7a0b\u4ea7\u751f\u7684\u6587\u4ef6\uff0c\u5373\u4f7f\u6587\u4ef6\u540d\u76f8\u540c\uff0c\u4e5f\u4e0d\u4fdd\u8bc1\u5177\u6709\u878d\u5408\u68c0\u6d4b\u9700\u8981\u7684\u4fe1\u606f\u3002\u5b98\u65b9\u8f93\u5165\u8bf4\u660e\u533a\u5206\u4e86\u628a\u5d4c\u5408\u8bb0\u5f55\u5199\u5165\u4e3b\u6587\u4ef6\u4e0e\u53e6\u5b58\u6587\u4ef6\u7684\u65b9\u5f0f\uff0c\u672c\u6b21\u4f7f\u7528\u524d\u4e00\u79cd\u3002\u76f8\u5173\u8981\u6c42\u89c1 <a style=\"color:#1671b8;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/suhrig\/arriba\/wiki\/04-Input-files\">Arriba \u8f93\u5165\u6587\u4ef6\u8bf4\u660e<\/a>\u3002<\/p>\n<p>\u5b9e\u8df5\u4e2d\u53ef\u4ee5\u5148\u4fdd\u5b58\u6bd4\u5bf9\u547d\u4ee4\u548c\u65e5\u5fd7\uff0c\u518d\u4fdd\u5b58\u878d\u5408\u547d\u4ee4\u3002\u8fd9\u6837\uff0c\u5f53\u7ed3\u679c\u4e3a\u7a7a\u65f6\uff0c\u53ef\u4ee5\u5224\u65ad\u95ee\u9898\u53d1\u751f\u5728\u5019\u9009\u751f\u6210\u4e4b\u524d\uff0c\u8fd8\u662f\u53d1\u751f\u5728\u540e\u7eed\u8fc7\u6ee4\u9636\u6bb5\u3002\u4ec5\u7559\u4e0b\u6700\u7ec8\u8868\u683c\uff0c\u4f1a\u8ba9\u4e24\u79cd\u5b8c\u5168\u4e0d\u540c\u7684\u5931\u8d25\u770b\u8d77\u6765\u4e00\u6837\u3002\u5bf9\u4e8e\u9700\u8981\u590d\u67e5\u7684\u5019\u9009\uff0c\u8bfb\u6bb5\u540d\u79f0\u8fd8\u80fd\u5e2e\u52a9\u56de\u5230\u539f\u59cb\u6570\u636e\uff0c\u4f46\u540d\u79f0\u5217\u8868\u672c\u8eab\u672a\u5fc5\u7b49\u540c\u4e8e\u8fc7\u6ee4\u540e\u7684\u6709\u6548\u6570\u91cf\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5b9e\u9a8c\u600e\u6837\u6784\u9020<\/h2>\n<p>\u6211\u4eec\u5b89\u88c5\u5e76\u8fd0\u884c\u4e86 Arriba 2.5.1\uff0c\u914d\u5408 STAR 2.7.11b\u3002\u53c2\u8003\u57fa\u56e0\u7ec4\u53ea\u6709\u4e24\u6761\u5404\u4e00\u4e07\u78b1\u57fa\u7684\u4eba\u5de5\u67d3\u8272\u4f53\uff0c\u6bcf\u6761\u5404\u653e\u7f6e\u4e00\u4e2a\u53cc\u5916\u663e\u5b50\u8f6c\u5f55\u672c\u3002\u878d\u5408\u8f6c\u5f55\u672c\u53d6\u7532\u57fa\u56e0\u7684\u7b2c\u4e00\u4e2a\u5916\u663e\u5b50\uff0c\u63a5\u4e0a\u4e59\u57fa\u56e0\u7684\u7b2c\u4e8c\u4e2a\u5916\u663e\u5b50\uff0c\u9884\u8bbe\u8fde\u63a5\u5750\u6807\u4e3a <code>chr1:1600<\/code> \u4e0e <code>chr2:4001<\/code>\u3002<\/p>\n<p>\u811a\u672c\u751f\u6210\u4e00\u767e\u516d\u5341\u5bf9\u6b63\u5e38\u8f6c\u5f55\u672c\u8bfb\u6bb5\u548c\u56db\u5341\u4e00\u5bf9\u878d\u5408\u8bfb\u6bb5\u3002\u6bcf\u7aef\u957f\u5ea6\u4e3a\u4e00\u767e\u4e94\u5341\u78b1\u57fa\uff0c\u7247\u6bb5\u957f\u5ea6\u4e3a\u4e09\u767e\u4e94\u5341\u78b1\u57fa\uff0c\u4e0d\u52a0\u5165\u6d4b\u5e8f\u9519\u8bef\u3002\u878d\u5408\u7247\u6bb5\u6cbf\u8fde\u63a5\u4f4d\u7f6e\u9519\u5f00\u8d77\u70b9\uff0c\u4f7f\u5b83\u4eec\u5177\u6709\u4e0d\u540c\u7684\u8986\u76d6\u65b9\u5f0f\u3002\u7b2c\u4e8c\u4efd\u8f93\u5165\u5728\u57fa\u7840\u6570\u636e\u4e0a\u989d\u5916\u52a0\u5165\u56db\u5341\u5bf9\u526f\u672c\uff0c\u8fd9\u4e9b\u526f\u672c\u6765\u81ea\u540c\u4e00\u6761\u878d\u5408\u7247\u6bb5\uff0c\u53ea\u6539\u540d\u79f0\uff0c\u4e0d\u6539\u5e8f\u5217\u53ca\u7247\u6bb5\u4f4d\u7f6e\u3002<\/p>\n<p>\u8fd9\u79cd\u8bbe\u8ba1\u628a\u53d8\u91cf\u9650\u5b9a\u5f97\u5f88\u6e05\u695a\u3002\u6b63\u5e38\u80cc\u666f\u3001\u53c2\u8003\u6587\u4ef6\u3001\u6bd4\u5bf9\u53c2\u6570\u548c\u539f\u6765\u7684\u878d\u5408\u7247\u6bb5\u5168\u90e8\u4fdd\u7559\uff0c\u65b0\u589e\u5185\u5bb9\u53ea\u662f\u91cd\u590d\u3002\u5b83\u4e0d\u6a21\u62df\u771f\u5b9e\u5b9e\u9a8c\u4e2d\u6240\u6709\u91cd\u590d\u6765\u6e90\uff0c\u4e5f\u4e0d\u8ba8\u8bba\u91cd\u590d\u4e00\u5b9a\u6765\u81ea\u6269\u589e\u8fd8\u662f\u9ad8\u8868\u8fbe\u3002\u6211\u4eec\u8981\u9a8c\u8bc1\u7684\u53ea\u662f\uff0c\u5728\u8fd9\u5957\u660e\u786e\u8f93\u5165\u4e0b\uff0c\u7a0b\u5e8f\u5982\u4f55\u8ba1\u6570\u4ee5\u53ca\u8f93\u51fa\u54ea\u4e9b\u8bca\u65ad\u4fe1\u606f\u3002<\/p>\n<p>\u672c\u6b21\u6ca1\u6709\u4f7f\u7528\u4eba\u7c7b\u9ed1\u540d\u5355\u3001\u5df2\u77e5\u878d\u5408\u5e93\u3001\u86cb\u767d\u7ed3\u6784\u57df\u6216\u57fa\u56e0\u7ec4\u7ed3\u6784\u53d8\u5f02\u3002\u4eba\u5de5\u53c2\u8003\u4e0e\u90a3\u4e9b\u8d44\u6e90\u6ca1\u6709\u5bf9\u5e94\u5173\u7cfb\uff0c\u56e0\u6b64\u5b9e\u9a8c\u5173\u95ed\u9ed1\u540d\u5355\u8fc7\u6ee4\uff0c\u5176\u4f59\u8fc7\u6ee4\u4fdd\u6301\u9ed8\u8ba4\uff0c\u53e6\u5916\u5b89\u6392\u4e00\u7ec4\u4e13\u95e8\u5173\u95ed\u91cd\u590d\u8fc7\u6ee4\u7684\u5bf9\u7167\u3002\u8fd9\u4e00\u8bbe\u7f6e\u53ea\u9002\u7528\u4e8e\u89e3\u91ca\u672c\u5b9e\u9a8c\uff0c\u4e0d\u80fd\u7167\u642c\u4e3a\u771f\u5b9e\u4eba\u7c7b\u6837\u672c\u7684\u9ed8\u8ba4\u65b9\u6848\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5b89\u88c5\u6210\u529f\u8981\u4ee5\u53ef\u6267\u884c\u548c\u7248\u672c\u8f93\u51fa\u4e3a\u51c6<\/h2>\n<p>\u9996\u6b21\u68c0\u67e5 STAR \u65f6\u9047\u5230\u4e00\u4e2a\u5bb9\u6613\u5ffd\u7565\u7684\u95ee\u9898\u3002\u5305\u5185\u540d\u4e3a STAR \u7684\u6587\u4ef6\u662f\u542f\u52a8\u5305\u88c5\u5668\uff0c\u5355\u72ec\u53d6\u51fa\u5b83\u4f1a\u56e0\u627e\u4e0d\u5230\u540c\u76ee\u5f55\u7684\u5b9e\u9645\u7a0b\u5e8f\u800c\u5931\u8d25\u3002\u672c\u6b21\u6539\u4e3a\u63d0\u53d6\u5305\u5185\u901a\u7528\u7248\u672c\u4e8c\u8fdb\u5236\uff0c\u5e76\u5728\u8bb0\u5f55\u4e2d\u6ce8\u660e\u5b9e\u9645\u6210\u5458\u3002\u968f\u540e\u5728\u56fa\u5b9a\u955c\u50cf\u63d0\u4f9b\u7684\u4f9d\u8d56\u73af\u5883\u4e2d\u8fd0\u884c\u7248\u672c\u67e5\u8be2\u3001\u7d22\u5f15\u751f\u6210\u3001\u6bd4\u5bf9\u53ca\u878d\u5408\u68c0\u6d4b\uff0c\u5168\u90e8\u6210\u529f\u3002<\/p>\n<p>\u8fd9\u4e00\u7ecf\u9a8c\u9002\u7528\u4e8e\u624b\u52a8\u63d0\u53d6\u8f6f\u4ef6\u5305\u7684\u573a\u666f\u3002\u4e0b\u8f7d\u5b8c\u6210\u4e0d\u7b49\u4e8e\u5b89\u88c5\u5b8c\u6210\uff0c\u770b\u5230\u53ef\u6267\u884c\u6587\u4ef6\u4e5f\u4e0d\u7b49\u4e8e\u4f9d\u8d56\u5b8c\u6574\u3002\u68c0\u67e5\u5e94\u81f3\u5c11\u5305\u542b\u7248\u672c\u8f93\u51fa\u548c\u4e00\u6b21\u80fd\u591f\u4ea7\u751f\u9884\u671f\u7ed3\u6784\u6587\u4ef6\u7684\u5c0f\u4efb\u52a1\u3002\u76f4\u63a5\u628a\u4e00\u4e2a\u6587\u4ef6\u590d\u5236\u5230\u7cfb\u7edf\u76ee\u5f55\u3001\u518d\u6839\u636e\u6587\u4ef6\u540d\u5224\u65ad\u7248\u672c\uff0c\u5bb9\u6613\u9057\u6f0f\u5305\u88c5\u5668\u3001\u52a8\u6001\u5e93\u548c\u4f34\u968f\u6570\u636e\u4e4b\u95f4\u7684\u5173\u7cfb\u3002<\/p>\n<p>\u672c\u6b21\u7684\u4e0b\u8f7d\u5730\u5740\u3001\u538b\u7f29\u5305\u6821\u9a8c\u503c\u3001\u4e8c\u8fdb\u5236\u6821\u9a8c\u503c\u548c\u8fd0\u884c\u955c\u50cf\u5747\u4fdd\u5b58\u5728\u7ed3\u679c\u8bb0\u5f55\u4e2d\u3002\u5b9e\u9a8c\u4f7f\u7528\u4e24\u4e2a\u8ba1\u7b97\u7ebf\u7a0b\u548c\u56db\u5409\u5b57\u8282\u7684\u5bb9\u5668\u4e0a\u9650\u3002\u8fd9\u53ea\u662f\u9694\u79bb\u7ec3\u4e60\u7684\u8fd0\u884c\u6761\u4ef6\uff0c\u4e0d\u80fd\u7528\u4e8e\u63a8\u7b97\u771f\u5b9e\u4eba\u7c7b\u6570\u636e\u7684\u5bb9\u91cf\u9700\u6c42\u3002\u5c0f\u53c2\u8003\u4e0a\u8fd1\u4e4e\u77ac\u65f6\u5b8c\u6210\u7684\u6b65\u9aa4\uff0c\u4e5f\u6ca1\u6709\u4f5c\u4e3a\u6027\u80fd\u6d4b\u8bd5\u53d1\u8868\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u6bd4\u5bf9\u65e5\u5fd7\u5148\u56de\u7b54\u7b2c\u4e00\u5c42\u95ee\u9898<\/h2>\n<p>\u57fa\u7840\u8f93\u5165\u5171\u6709\u4e8c\u767e\u96f6\u4e00\u5bf9\u8bfb\u6bb5\uff0c\u5176\u4e2d\u4eba\u4e3a\u6784\u9020\u7684\u878d\u5408\u7247\u6bb5\u4e3a\u56db\u5341\u4e00\u5bf9\u3002STAR \u65e5\u5fd7\u62a5\u544a\u7684\u5d4c\u5408\u8bfb\u6bb5\u6570\u4e3a\u4e09\u5341\u4e03\uff0cArriba \u968f\u540e\u8bfb\u53d6\u5230\u7684\u5d4c\u5408\u8bb0\u5f55\u4e5f\u662f\u4e09\u5341\u4e03\u3002\u6700\u7ec8\u4fdd\u7559\u7684\u76ee\u6807\u878d\u5408\u6070\u597d\u6709\u4e09\u5341\u4e03\u4e2a\u6709\u6548\u652f\u6301\u7247\u6bb5\u3002<\/p>\n<p>\u8fd9\u4e2a\u8fc7\u7a0b\u63d0\u793a\u6211\u4eec\uff0c\u77e5\u9053\u751f\u6210\u4e86\u591a\u5c11\u878d\u5408\u7247\u6bb5\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u4e0b\u6e38\u4e00\u5b9a\u63a5\u6536\u5230\u540c\u6837\u6570\u91cf\u7684\u878d\u5408\u8bc1\u636e\u3002\u6bd4\u5bf9\u5bf9\u951a\u5b9a\u957f\u5ea6\u3001\u4f4d\u7f6e\u548c\u5d4c\u5408\u62a5\u544a\u65b9\u5f0f\u6709\u81ea\u5df1\u7684\u9650\u5236\u3002\u8fd9\u91cc\u6ca1\u6709\u628a\u5c11\u6389\u7684\u56db\u4e2a\u7247\u6bb5\u5f52\u548e\u4e8e\u67d0\u4e00\u4e2a\u672a\u7ecf\u9010\u6761\u9a8c\u8bc1\u7684\u539f\u56e0\uff0c\u4e5f\u6ca1\u6709\u5199\u6210 Arriba \u4e22\u6389\u4e86\u56db\u4e2a\u771f\u9633\u6027\uff0c\u56e0\u4e3a\u65e5\u5fd7\u5df2\u7ecf\u663e\u793a\u5dee\u5f02\u53d1\u751f\u5728\u5b83\u63a5\u6536\u5019\u9009\u4e4b\u524d\u3002<\/p>\n<p>\u7b2c\u4e8c\u4efd\u8f93\u5165\u5171\u6709\u4e8c\u767e\u56db\u5341\u4e00\u5bf9\u8bfb\u6bb5\u3002Arriba \u8bfb\u53d6\u7684\u5d4c\u5408\u8bb0\u5f55\u589e\u52a0\u5230\u4e03\u5341\u4e03\uff0c\u6267\u884c\u91cd\u590d\u8fc7\u6ee4\u540e\u53c8\u56de\u5230\u4e09\u5341\u4e03\u3002\u8fd9\u4e2a\u4e2d\u95f4\u65e5\u5fd7\u628a\u56e0\u679c\u94fe\u8865\u9f50\u4e86\u3002\u82e5\u53ea\u6bd4\u8f83\u6700\u7ec8\u4e24\u5f20\u8868\uff0c\u53ea\u80fd\u770b\u5230\u8ba1\u6570\u76f8\u540c\u3002\u8fde\u540c\u6bd4\u5bf9\u53ca\u8fc7\u6ee4\u65e5\u5fd7\u4e00\u8d77\u67e5\u770b\uff0c\u624d\u80fd\u786e\u8ba4\u65b0\u589e\u526f\u672c\u786e\u5b9e\u8fdb\u5165\u4e86\u6d41\u7a0b\uff0c\u53c8\u88ab\u54ea\u4e2a\u6b65\u9aa4\u8bc6\u522b\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u540c\u4e00\u5019\u9009\u7684\u4e09\u7ec4\u7ed3\u679c<\/h2>\n<p>\u4e09\u7ec4\u5747\u4fdd\u7559\u4e00\u6761\u7532\u57fa\u56e0\u5230\u4e59\u57fa\u56e0\u7684\u76ee\u6807\u878d\u5408\uff0c\u65ad\u70b9\u4e0e\u9884\u8bbe\u4f4d\u7f6e\u4e00\u81f4\u3002\u4e0b\u9762\u53ea\u6458\u53d6\u89e3\u91ca\u91cd\u590d\u6548\u5e94\u6240\u9700\u7684\u5b57\u6bb5\u3002\u4e24\u7c7b\u5206\u88c2\u652f\u6301\u5206\u522b\u5bf9\u5e94\u7ed3\u679c\u4e2d\u7684 <code>split_reads1<\/code> \u548c <code>split_reads2<\/code>\uff0c\u8de8\u63a5\u652f\u6301\u5bf9\u5e94 <code>discordant_mates<\/code>\u3002<\/p>\n<div class=\"guide-table\" style=\"max-width:100%;overflow-x:auto;margin:18px 0\">\n<table style=\"display:table;width:100%;max-width:100%;min-width:0;table-layout:fixed;border-collapse:collapse;font-size:.92em;line-height:1.6\">\n<thead>\n<tr>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eef7ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u8fd0\u884c\u6761\u4ef6<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eef7ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u5206\u88c2\u652f\u6301\u7532\uff0f\u4e59<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eef7ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u8de8\u63a5\u652f\u6301<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eef7ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u6709\u6548\u652f\u6301\u5408\u8ba1<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u57fa\u7840\u8f93\u5165<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">12\uff0f17<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">8<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">37<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u589e\u52a0\u526f\u672c\u5e76\u8fc7\u6ee4<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">12\uff0f17<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">8<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">37<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u589e\u526f\u672c\u4e0d\u53bb\u91cd<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">12\uff0f57<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">8<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">77<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>\u5173\u95ed\u91cd\u590d\u8fc7\u6ee4\u540e\uff0c\u589e\u52a0\u7684\u56db\u5341\u4e2a\u652f\u6301\u5168\u90e8\u8fdb\u5165\u4e59\u4fa7\u5206\u88c2\u652f\u6301\u680f\u3002\u65ad\u70b9\u6ca1\u6709\u56e0\u6b64\u53d8\u5f97\u66f4\u7cbe\u786e\uff0c\u53c2\u8003\u5e8f\u5217\u4e5f\u6ca1\u6709\u589e\u52a0\u65b0\u4fe1\u606f\u3002\u8fd9\u662f\u4e00\u4e2a\u76f4\u63a5\u7684\u53cd\u4f8b\uff0c\u8bf4\u660e\u652f\u6301\u6570\u53d8\u5316\u5fc5\u987b\u7ed3\u5408\u9884\u5904\u7406\u548c\u8ba1\u6570\u89c4\u5219\u89e3\u91ca\u3002\u540c\u4e00\u4e2a\u9608\u503c\u7528\u4e8e\u4e24\u5957\u8fc7\u6ee4\u89c4\u5219\u4e0d\u540c\u7684\u7ed3\u679c\u65f6\uff0c\u542b\u4e49\u53ef\u80fd\u5df2\u7ecf\u6539\u53d8\u3002<\/p>\n<div class=\"guide-table\" style=\"max-width:100%;overflow-x:auto;margin:18px 0\">\n<table style=\"display:table;width:100%;max-width:100%;min-width:0;table-layout:fixed;border-collapse:collapse;font-size:.92em;line-height:1.6\">\n<thead>\n<tr>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eef7ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u8fd0\u884c\u6761\u4ef6<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eef7ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u7532\u4fa7\u8986\u76d6\u5ea6<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eef7ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u4e59\u4fa7\u8986\u76d6\u5ea6<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eef7ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u8fc7\u6ee4\u680f<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u57fa\u7840\u8f93\u5165<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">47<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">54<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u65e0<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u589e\u52a0\u526f\u672c\u5e76\u8fc7\u6ee4<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">47<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">94<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u91cd\u590d\u526f\u672c 40<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u589e\u52a0\u526f\u672c\u4f46\u4e0d\u53bb\u91cd<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">47<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">94<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u65e0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>\u8986\u76d6\u5ea6\u4e0e\u6709\u6548\u652f\u6301\u6570\u9075\u5faa\u4e0d\u540c\u7684\u8ba1\u7b97\u53e3\u5f84\uff0c\u5b98\u65b9\u4e5f\u63d0\u9192\u8986\u76d6\u5ea6\u8ba1\u7b97\u5305\u542b\u91cd\u590d\u7247\u6bb5\u3002\u5b57\u6bb5\u5b9a\u4e49\u53ef\u67e5 <a style=\"color:#1671b8;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/suhrig\/arriba\/wiki\/05-Output-files\">Arriba \u8f93\u51fa\u8bf4\u660e<\/a>\u3002\u5728\u672c\u6b21\u5bf9\u7167\u4e2d\uff0c\u53ea\u6709\u4e59\u4fa7\u8986\u76d6\u5ea6\u589e\u52a0\uff0c\u4e0d\u80fd\u8fdb\u4e00\u6b65\u63a8\u5e7f\u6210\u6bcf\u4e2a\u91cd\u590d\u7247\u6bb5\u90fd\u4f1a\u540c\u65f6\u589e\u52a0\u4e24\u4fa7\u8986\u76d6\u5ea6\u3002\u5e94\u6839\u636e\u5b9e\u9645\u8f93\u51fa\u548c\u6bd4\u5bf9\u4f4d\u7f6e\u89e3\u91ca\u5177\u4f53\u53d8\u5316\u3002<\/p>\n<p>\u7531\u6b64\u8fd8\u53ef\u4ee5\u68c0\u9a8c\u4e00\u79cd\u5e38\u89c1\u4f46\u4e0d\u4e25\u8c28\u7684\u505a\u6cd5\u3002\u5982\u679c\u5c06\u6709\u6548\u652f\u6301\u9664\u4ee5\u4e24\u4fa7\u8986\u76d6\u5ea6\u4e4b\u548c\uff0c\u57fa\u7840\u8f93\u5165\u5f97\u5230\u7ea6\u96f6\u70b9\u4e09\u516d\u516d\uff0c\u52a0\u5165\u526f\u672c\u5e76\u8fc7\u6ee4\u540e\u7ea6\u4e3a\u96f6\u70b9\u4e8c\u516d\u4e8c\u3002\u878d\u5408\u5e8f\u5217\u5e76\u672a\u6539\u53d8\uff0c\u6bd4\u503c\u5374\u4e0b\u964d\u4e86\u3002\u8fd9\u4e2a\u6bd4\u503c\u4e0d\u80fd\u672a\u7ecf\u5b9a\u4e49\u5c31\u547d\u540d\u4e3a\u80bf\u7624\u7ec6\u80de\u6bd4\u4f8b\u3001\u878d\u5408\u7b49\u4f4d\u57fa\u56e0\u9891\u7387\u6216\u771f\u5b9e\u8f6c\u5f55\u672c\u5360\u6bd4\uff0c\u56e0\u4e3a\u5206\u5b50\u548c\u5206\u6bcd\u5e76\u4e0d\u662f\u540c\u4e00\u79cd\u8ba1\u6570\u5bf9\u8c61\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u540d\u79f0\u5217\u8868\u4e5f\u9700\u8981\u6838\u5bf9\u53e3\u5f84<\/h2>\n<p>\u66f4\u5bb9\u6613\u6f0f\u770b\u7684\u73b0\u8c61\u51fa\u73b0\u5728\u8bfb\u6bb5\u540d\u79f0\u680f\u3002\u57fa\u7840\u8f93\u5165\u7684\u540d\u79f0\u5217\u8868\u6709\u4e09\u5341\u4e03\u9879\uff0c\u589e\u52a0\u526f\u672c\u5e76\u6267\u884c\u8fc7\u6ee4\u540e\uff0c\u5217\u8868\u4ecd\u5305\u542b\u4e03\u5341\u4e03\u4e2a\u540d\u79f0\uff0c\u800c\u6709\u6548\u652f\u6301\u6570\u4fdd\u6301\u4e09\u5341\u4e03\u3002\u4e5f\u5c31\u662f\u8bf4\uff0c\u672c\u6b21\u8f93\u51fa\u4e2d\u7684\u540d\u79f0\u5217\u8868\u4fdd\u7559\u4e86\u88ab\u6807\u8bb0\u91cd\u590d\u7684\u76f8\u5173\u8bfb\u6bb5\uff0c\u5e76\u4e0d\u662f\u4e00\u4efd\u5df2\u7ecf\u6392\u9664\u5168\u90e8\u8fc7\u6ee4\u8bc1\u636e\u7684\u72ec\u7acb\u7247\u6bb5\u6e05\u5355\u3002<\/p>\n<p>\u56e0\u6b64\uff0c\u5199\u7edf\u8ba1\u811a\u672c\u65f6\u4e0d\u8981\u7b80\u5355\u5bf9\u9017\u53f7\u5206\u9694\u540e\u7684\u540d\u79f0\u8ba1\u6570\uff0c\u518d\u628a\u7ed3\u679c\u8986\u76d6\u5230\u652f\u6301\u6570\u5b57\u6bb5\u3002\u90a3\u6837\u4f1a\u628a\u7a0b\u5e8f\u5df2\u7ecf\u5904\u7406\u8fc7\u7684\u526f\u672c\u91cd\u65b0\u7b97\u8fdb\u53bb\u3002\u540d\u79f0\u66f4\u9002\u5408\u4f5c\u4e3a\u56de\u67e5\u5165\u53e3\u3002\u8981\u7edf\u8ba1\u6700\u7ec8\u8bc1\u636e\uff0c\u5e94\u5148\u660e\u786e\u6240\u7528\u5b57\u6bb5\u4e0e\u8fc7\u6ee4\u72b6\u6001\uff0c\u5e76\u5728\u5c11\u91cf\u5019\u9009\u4e0a\u505a\u4eba\u5de5\u5bf9\u7167\uff0c\u786e\u8ba4\u81ea\u7f16\u4ee3\u7801\u6ca1\u6709\u6084\u6084\u6539\u53d8\u53e3\u5f84\u3002<\/p>\n<p>\u8fd9\u4e2a\u6838\u5bf9\u8fd8\u53ef\u4ee5\u5e2e\u52a9\u53d1\u73b0\u53e6\u4e00\u4e2a\u95ee\u9898\u3002\u6700\u7ec8\u5019\u9009\u53ea\u6709\u4e00\u884c\uff0c\u4e0d\u4ee3\u8868\u4e2d\u95f4\u53ea\u751f\u6210\u8fc7\u4e00\u4e2a\u5019\u9009\u3002\u672c\u6b21\u65e5\u5fd7\u4e00\u5ea6\u8bb0\u5f55\u4e5d\u4e2a\u5019\u9009\uff0c\u7ecf\u8fc7\u76f8\u5bf9\u652f\u6301\u8fc7\u6ee4\u540e\u5269\u4e0b\u4e00\u6761\u3002\u88ab\u4e22\u5f03\u8868\u4fdd\u7559\u4e86\u5ba1\u67e5\u5165\u53e3\u3002\u6574\u7406\u7ed3\u679c\u65f6\u540c\u65f6\u4fdd\u7559\u4e3b\u8868\u4e0e\u88ab\u4e22\u5f03\u8868\uff0c\u6bd4\u53ea\u590d\u5236\u901a\u8fc7\u7684\u90a3\u4e00\u884c\u66f4\u6709\u5229\u4e8e\u89e3\u91ca\u4e3a\u4ec0\u4e48\u67d0\u4e2a\u90bb\u8fd1\u65ad\u70b9\u6ca1\u6709\u8fdb\u5165\u6700\u7ec8\u7ed3\u679c\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u9ad8\u7f6e\u4fe1\u5ea6\u548c\u7f3a\u5931\u5b57\u6bb5\u5206\u522b\u610f\u5473\u7740\u4ec0\u4e48<\/h2>\n<p>\u4e09\u7ec4\u76ee\u6807\u878d\u5408\u90fd\u6807\u4e3a\u9ad8\u7f6e\u4fe1\u5ea6\uff0c\u5173\u95ed\u91cd\u590d\u8fc7\u6ee4\u5e76\u6ca1\u6709\u8ba9\u8fd9\u4e2a\u6807\u7b7e\u53d1\u751f\u53d8\u5316\u3002\u6807\u7b7e\u662f\u79bb\u6563\u5206\u7ea7\uff0c\u4e0d\u662f\u6bcf\u589e\u52a0\u4e00\u4e2a\u8bfb\u6bb5\u5c31\u8fde\u7eed\u53d8\u5316\u7684\u6570\u503c\u3002\u4e5f\u4e0d\u80fd\u628a\u9ad8\u7f6e\u4fe1\u5ea6\u7406\u89e3\u4e3a\u6709\u7279\u5b9a\u81f4\u75c5\u4f5c\u7528\u3002\u5b98\u65b9\u660e\u786e\u533a\u5206\u5019\u9009\u53ef\u4fe1\u7a0b\u5ea6\u4e0e\u80bf\u7624\u751f\u7269\u5b66\u610f\u4e49\uff0c\u53c2\u89c1 <a style=\"color:#1671b8;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/suhrig\/arriba\/wiki\/08-Interpretation-of-results\">Arriba \u7ed3\u679c\u89e3\u91ca<\/a>\u3002<\/p>\n<p>\u672c\u6b21\u6ce8\u91ca\u6ca1\u6709\u63d0\u4f9b\u7f16\u7801\u533a\u548c\u7ed3\u6784\u57df\u4fe1\u606f\uff0c\u56e0\u6b64\u9605\u8bfb\u6846\u3001\u80bd\u5e8f\u5217\u548c\u7ed3\u6784\u57df\u76f8\u5173\u5b57\u6bb5\u6ca1\u6709\u7ed9\u51fa\u6709\u6548\u9884\u6d4b\u3002\u9762\u5bf9\u8fd9\u6837\u7684\u7a7a\u503c\uff0c\u6b63\u786e\u7ed3\u8bba\u662f\u5f53\u524d\u8f93\u5165\u4e0e\u8fd0\u884c\u6ca1\u6709\u63d0\u4f9b\u76f8\u5e94\u5224\u65ad\u4f9d\u636e\u3002\u5b83\u4e0d\u80fd\u6539\u5199\u4e3a\u4e0d\u7f16\u7801\u86cb\u767d\u3001\u6ca1\u6709\u529f\u80fd\u7ed3\u6784\u57df\uff0c\u6216\u4e00\u5b9a\u4e0d\u80fd\u5f62\u6210\u878d\u5408\u86cb\u767d\u3002\u7a7a\u503c\u7684\u89e3\u91ca\u5e94\u5f53\u56de\u5230\u8f93\u5165\u6761\u4ef6\uff0c\u800c\u4e0d\u662f\u4ec5\u51ed\u8868\u683c\u4e2d\u7684\u4e00\u4e2a\u70b9\u53f7\u4e0b\u7ed3\u8bba\u3002<\/p>\n<p>\u540c\u7406\uff0c\u4e24\u7aef\u843d\u5728\u5916\u663e\u5b50\u8fb9\u754c\u53ea\u80fd\u8bf4\u660e\u4e0e\u672c\u6b21\u6ce8\u91ca\u4e2d\u7684\u4f4d\u7f6e\u543b\u5408\u3002\u82e5\u8981\u8fdb\u4e00\u6b65\u7814\u7a76\u529f\u80fd\uff0c\u9700\u8981\u5177\u4f53\u8f6c\u5f55\u672c\u3001\u7f16\u7801\u76f8\u4f4d\u53ca\u8fde\u63a5\u540e\u5e8f\u5217\u7b49\u4fe1\u606f\u3002\u82e5\u8981\u9a8c\u8bc1\u771f\u5b9e\u6837\u672c\u4e2d\u7684\u4e8b\u4ef6\uff0c\u8fd8\u9700\u8981\u72ec\u7acb\u8bc1\u636e\u3002\u628a\u68c0\u6d4b\u3001\u6ce8\u91ca\u548c\u751f\u7269\u5b66\u89e3\u91ca\u5206\u5f00\uff0c\u80fd\u8ba9\u7ed3\u679c\u62a5\u544a\u66f4\u51c6\u786e\uff0c\u4e5f\u4fbf\u4e8e\u540e\u7eed\u77e5\u9053\u4e0b\u4e00\u6b65\u5e94\u8865\u4ec0\u4e48\u6570\u636e\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u600e\u6837\u590d\u73b0\u5e76\u8fc1\u79fb\u5230\u81ea\u5df1\u7684\u6d41\u7a0b<\/h2>\n<p>\u5b8c\u6574\u811a\u672c\u4f1a\u751f\u6210\u53c2\u8003\u4e0e\u8bfb\u6bb5\u3001\u5b89\u88c5\u6240\u9700\u53ef\u6267\u884c\u7a0b\u5e8f\u3001\u5efa\u7acb\u7d22\u5f15\u3001\u5b8c\u6210\u4e24\u4efd\u8f93\u5165\u7684\u6bd4\u5bf9\u5e76\u6267\u884c\u4e09\u7ec4 Arriba \u5bf9\u7167\u3002\u4e0b\u8f7d <a style=\"color:#1671b8;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/ysx-bioinformatics-practice-156-158-497c88eef8e3.zip\">\u672c\u7ec4\u5b9e\u8dd1\u811a\u672c\u4e0e\u7ed3\u679c\u8bb0\u5f55<\/a> \u540e\uff0c\u5728\u6709 Docker \u7684 Linux \u73af\u5883\u8fd0\u884c\u5bf9\u5e94\u76ee\u5f55\u4e2d\u7684 run.py \u9a71\u52a8\u811a\u672c\u5373\u53ef\u3002\u538b\u7f29\u5305\u4e2d\u7684\u8bb0\u5f55\u5305\u542b\u5b9e\u9645\u8f93\u51fa\uff0c\u800c\u4e0d\u662f\u53ea\u6709\u9884\u671f\u7b54\u6848\u3002<\/p>\n<pre style=\"max-width:100%;box-sizing:border-box;overflow-x:auto;margin:18px 0;padding:14px 16px;background:#f3f6fa;color:#1a293c;border:1px solid #d8e1ea;border-radius:8px;line-height:1.55;white-space:pre;overflow-wrap:normal;word-break:normal\"><code class=\"language-bash\">python3 run.py run156\r\n<\/code><\/pre>\n<p>\u5b9e\u9a8c\u91cc Arriba \u7684\u5173\u952e\u5bf9\u7167\u547d\u4ee4\u5982\u4e0b\uff0c\u8f93\u5165\u6587\u4ef6\u7531\u9a71\u52a8\u811a\u672c\u751f\u6210\u3002\u771f\u5b9e\u6570\u636e\u5e94\u5148\u6309\u81ea\u5df1\u7684\u7269\u79cd\u3001\u53c2\u8003\u548c\u6ce8\u91ca\u51c6\u5907\u8d44\u6e90\uff0c\u4e0d\u8981\u76f4\u63a5\u6cbf\u7528\u8fd9\u91cc\u5173\u95ed\u9ed1\u540d\u5355\u7684\u6559\u5b66\u8bbe\u7f6e\u3002<\/p>\n<pre style=\"max-width:100%;box-sizing:border-box;overflow-x:auto;margin:18px 0;padding:14px 16px;background:#f3f6fa;color:#1a293c;border:1px solid #d8e1ea;border-radius:8px;line-height:1.55;white-space:pre;overflow-wrap:normal;word-break:normal\"><code class=\"language-bash\">.\/arriba -x duplicates\/Aligned.out.bam \\\r\n  -a genome.fa -g genes.gtf \\\r\n  -o duplicates_filtered.tsv \\\r\n  -O duplicates_filtered.discarded.tsv -f blacklist\r\n\r\n.\/arriba -x duplicates\/Aligned.out.bam \\\r\n  -a genome.fa -g genes.gtf \\\r\n  -o duplicates_keep_duplicates.tsv \\\r\n  -O duplicates_keep_duplicates.discarded.tsv \\\r\n  -f blacklist,duplicates\r\n<\/code><\/pre>\n<p>\u8fc1\u79fb\u6d41\u7a0b\u65f6\uff0c\u5efa\u8bae\u5148\u7528\u4e00\u4efd\u5df2\u77e5\u6765\u6e90\u7684\u5c0f\u8f93\u5165\u68c0\u67e5\u5b57\u6bb5\u53ca\u8ba1\u6570\u5173\u7cfb\uff0c\u518d\u6269\u5927\u6570\u636e\u91cf\u3002\u68c0\u67e5\u76ee\u6807\u5e94\u662f\u7ed3\u679c\u80fd\u5426\u8ffd\u6eaf\uff0c\u800c\u4e0d\u53ea\u662f\u7a0b\u5e8f\u662f\u5426\u8fd4\u56de\u6210\u529f\u3002\u81f3\u5c11\u8981\u80fd\u56de\u7b54\u8f93\u5165\u542b\u591a\u5c11\u7247\u6bb5\u3001\u4e0a\u6e38\u62a5\u544a\u591a\u5c11\u5d4c\u5408\u8bc1\u636e\u3001\u4e0b\u6e38\u4fdd\u7559\u591a\u5c11\u6709\u6548\u652f\u6301\u3001\u54ea\u4e9b\u8fc7\u6ee4\u89e3\u91ca\u4e86\u5dee\u989d\uff0c\u4ee5\u53ca\u6700\u7ec8\u5019\u9009\u5bf9\u5e94\u54ea\u4e00\u7248\u53c2\u8003\u4e0e\u6ce8\u91ca\u3002<\/p>\n<p>\u672c\u6b21\u8fd8\u51fa\u73b0\u4e86\u65e0\u6cd5\u7528\u5c11\u91cf\u5d4c\u5408\u8bfb\u6bb5\u4f30\u8ba1\u7247\u6bb5\u95f4\u9694\u5206\u5e03\u7684\u63d0\u793a\uff0c\u7a0b\u5e8f\u4f7f\u7528\u4e86\u9ed8\u8ba4\u503c\u3002\u6211\u4eec\u4fdd\u7559\u4e86\u8fd9\u4e2a\u63d0\u793a\uff0c\u6ca1\u6709\u56e0\u4e3a\u4efb\u52a1\u5b8c\u6210\u5c31\u628a\u5b83\u5ffd\u7565\u3002\u5b83\u63d0\u9192\u8bfb\u8005\uff0c\u5c0f\u5b9e\u9a8c\u53ef\u4ee5\u9a8c\u8bc1\u5b57\u6bb5\u548c\u63a7\u5236\u53d8\u91cf\uff0c\u5374\u4e0d\u80fd\u8986\u76d6\u5927\u6837\u672c\u4e2d\u7684\u5168\u90e8\u4f30\u8ba1\u8fc7\u7a0b\u3002\u5c06\u8fd9\u7c7b\u9650\u5236\u5199\u5728\u7ed3\u679c\u65c1\u8fb9\uff0c\u6bd4\u628a\u4e00\u6761\u6210\u529f\u547d\u4ee4\u5305\u88c5\u6210\u901a\u7528\u53c2\u6570\u66f4\u6709\u7528\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u4f18\u70b9\u3001\u5c40\u9650\u4e0e\u5b9e\u9645\u9009\u7528<\/h2>\n<p>Arriba \u7684\u5b9e\u7528\u4f18\u70b9\u662f\u80fd\u591f\u628a\u5019\u9009\u3001\u652f\u6301\u7c7b\u578b\u548c\u8fc7\u6ee4\u539f\u56e0\u653e\u5728\u53ef\u8ffd\u6eaf\u7684\u8f93\u51fa\u4e2d\u3002\u672c\u6b21\u91cd\u590d\u5bf9\u7167\u6b63\u662f\u9760\u8fd9\u4e9b\u4fe1\u606f\u89e3\u91ca\u6e05\u695a\u7684\u3002\u5b83\u9002\u5408\u5df2\u6709\u5408\u9002\u6bd4\u5bf9\u6587\u4ef6\u3001\u53c8\u9700\u8981\u9010\u6761\u5ba1\u9605\u878d\u5408\u5019\u9009\u7684\u6d41\u7a0b\u3002\u5b98\u65b9\u63d0\u4f9b\u7684\u5b8c\u6574\u6d41\u7a0b\u53ef\u4ee5\u4f5c\u4e3a\u914d\u7f6e\u5165\u53e3\uff0c\u89c1 <a style=\"color:#1671b8;text-decoration:underline;text-underline-offset:2px\" 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