{"id":73173,"date":"2026-11-15T14:33:36","date_gmt":"2026-11-15T06:33:36","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73173"},"modified":"2026-11-15T14:33:36","modified_gmt":"2026-11-15T06:33:36","slug":"star-fusion-ffpm-estimated-count-denominator-threshold-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/star-fusion-ffpm-estimated-count-denominator-threshold-practice\/","title":{"rendered":"STAR-Fusion \u7ed3\u679c\u5904\u7406\u5b9e\u8dd1\uff0cFFPM \u7684\u5206\u5b50\u3001\u5206\u6bcd\u4e0e\u9608\u503c\u600e\u6837\u5f71\u54cd\u5019\u9009\u540d\u5355"},"content":{"rendered":"
\n

\u4e24\u4e2a\u878d\u5408\u5019\u9009\u7684\u539f\u59cb\u652f\u6301\u6570\u5b8c\u5168\u4e00\u6837\uff0c\u6700\u7ec8\u7684\u6807\u51c6\u5316\u6570\u503c\u5374\u76f8\u5dee\u4e94\u500d\uff0c\u662f\u5426\u8bf4\u660e\u7a0b\u5e8f\u7b97\u9519\u4e86\u3002\u53e6\u4e00\u6761\u5019\u9009\u6ca1\u6709\u5206\u88c2\u8bfb\u6bb5\uff0c\u53ea\u51ed\u8de8\u63a5\u7247\u6bb5\u5c31\u901a\u8fc7\u4e86\u67d0\u4e2a\u6570\u503c\u9608\u503c\uff0c\u662f\u5426\u8bf4\u660e\u5b83\u5df2\u7ecf\u88ab\u8bc1\u5b9e\u3002\u8fd9\u4e24\u7c7b\u95ee\u9898\u7ecf\u5e38\u51fa\u73b0\u5728\u7ed3\u679c\u6574\u7406\u9636\u6bb5\uff0c\u6839\u6e90\u662f\u628a\u4e0d\u540c\u5b57\u6bb5\u3001\u4e0d\u540c\u8fc7\u6ee4\u9636\u6bb5\u548c\u4e0d\u540c\u8bc1\u636e\u5f3a\u5ea6\u6df7\u5728\u4e86\u4e00\u8d77\u3002<\/p>\n

\u672c\u6b21\u5b89\u88c5 STAR-Fusion 1.15.1 \u7684\u5b98\u65b9\u7ed3\u679c\u5904\u7406\u811a\u672c\uff0c\u5728\u660e\u786e\u865a\u6784\u7684\u5019\u9009\u8868\u4e0a\u8fd0\u884c\u6807\u51c6\u5316\u8ba1\u7b97\u548c\u9608\u503c\u8fc7\u6ee4\u3002\u91cd\u70b9\u662f\u7406\u89e3\u7ed3\u679c\u5904\u7406\u7684\u7b97\u672f\u4e0e\u8fb9\u754c\u3002\u6211\u4eec\u6ca1\u6709\u8fdb\u884c\u771f\u5b9e\u8bfb\u6bb5\u6bd4\u5bf9\uff0c\u6ca1\u6709\u5efa\u7acb\u53c2\u8003\u5e93\uff0c\u6ca1\u6709\u8fd0\u884c\u878d\u5408\u53d1\u73b0\u3001\u6b67\u4e49\u5206\u914d\u6216 FusionInspector\uff0c\u4e5f\u6ca1\u6709\u628a\u811a\u672c\u6d4b\u8bd5\u79f0\u4e3a\u5b8c\u6574\u878d\u5408\u68c0\u6d4b\u5b9e\u6d4b\u3002<\/p>\n

\"\u540c\u6837\u7684\u539f\u59cb\u652f\u6301\uff0cFFPM
\u5b98\u65b9\u7ed3\u679c\u811a\u672c\u5b9e\u8dd1 \u00b7 \u8f93\u5165\u8ba1\u6570\u4e0e\u5206\u6bcd\u5747\u4e3a\u4eba\u5de5\u8bbe\u5b9a<\/figcaption><\/figure>\n

\u5148\u5206\u6e05\u5b8c\u6574\u6d41\u7a0b\u548c\u4e00\u4e2a\u7ed3\u679c\u5b57\u6bb5<\/h2>\n

STAR-Fusion \u7684\u5b8c\u6574\u5de5\u4f5c\u6d89\u53ca\u6bd4\u5bf9\u3001\u5019\u9009\u751f\u6210\u3001\u6ce8\u91ca\u4e0e\u8fc7\u6ee4\u3002\u5b83\u65e2\u53ef\u4ee5\u4ece\u8bfb\u6bb5\u6587\u4ef6\u5f00\u59cb\uff0c\u4e5f\u53ef\u4ee5\u63a5\u6536\u5df2\u6709\u7684\u5d4c\u5408\u8fde\u63a5\u6587\u4ef6\uff0c\u4f46\u540e\u8005\u4ecd\u9700\u8981\u4e0e\u6d41\u7a0b\u5339\u914d\u7684\u53c2\u8003\u8d44\u6e90\u3002\u5b98\u65b9\u8fd8\u8981\u6c42\u76f8\u5e94\u6bd4\u5bf9\u8f93\u51fa\u5e26\u6709\u8ba1\u7b97\u6240\u9700\u7684\u7edf\u8ba1\u4fe1\u606f\u3002\u5165\u53e3\u4e0e\u53c2\u6570\u8bf4\u660e\u89c1 STAR-Fusion \u5b98\u65b9\u5de5\u4f5c\u6d41\u7a0b<\/a>\u3002<\/p>\n

\u5bf9\u4f7f\u7528\u8005\u800c\u8a00\uff0c\u6700\u9700\u8981\u907f\u514d\u7684\u662f\u628a\u67d0\u4e2a\u540e\u5904\u7406\u811a\u672c\u7684\u6210\u529f\u5f53\u6210\u6574\u6761\u6d41\u7a0b\u6210\u529f\u3002\u80fd\u591f\u7ed9\u4e00\u5f20\u8868\u52a0\u4e0a\u6807\u51c6\u5316\u6570\u5b57\uff0c\u53ea\u80fd\u8bc1\u660e\u8fd9\u5f20\u8868\u88ab\u5f53\u524d\u811a\u672c\u5904\u7406\u4e86\uff0c\u4e0d\u80fd\u8bc1\u660e\u8868\u4e2d\u7684\u5019\u9009\u6765\u81ea\u771f\u5b9e\u6bd4\u5bf9\uff0c\u66f4\u4e0d\u80fd\u8bc1\u660e\u5019\u9009\u901a\u8fc7\u4e86\u5168\u90e8\u4e0a\u6e38\u8fc7\u6ee4\u3002\u5b9e\u9645\u9879\u76ee\u5e94\u8bb0\u5f55\u6bcf\u4e2a\u7ed3\u679c\u6587\u4ef6\u7684\u751f\u6210\u6b65\u9aa4\uff0c\u800c\u4e0d\u662f\u4ec5\u51ed\u6587\u4ef6\u540d\u91cc\u5e26\u6709\u6700\u7ec8\u7ed3\u679c\u5b57\u6837\u6765\u5224\u65ad\u3002<\/p>\n

\u672c\u5b9e\u9a8c\u9009\u62e9\u8f83\u5c0f\u7684\u5207\u5165\u70b9\uff0c\u662f\u4e3a\u4e86\u628a\u8f93\u5165\u4e0e\u8f93\u51fa\u4e4b\u95f4\u7684\u5173\u7cfb\u5b8c\u5168\u5c55\u793a\u51fa\u6765\u3002\u6240\u6709\u5019\u9009\u540d\u79f0\u90fd\u662f\u4eba\u5de5\u547d\u540d\uff0c\u8ba1\u6570\u548c\u6587\u5e93\u5206\u6bcd\u4e5f\u662f\u4eba\u4e3a\u8bbe\u5b9a\u3002\u8fd9\u6837\u53ef\u4ee5\u4e3b\u52a8\u6539\u53d8\u5355\u4e2a\u6761\u4ef6\uff0c\u68c0\u67e5\u811a\u672c\u5230\u5e95\u8bfb\u53d6\u54ea\u4e00\u5217\u3001\u5982\u4f55\u820d\u5165\u4ee5\u53ca\u600e\u6837\u6bd4\u8f83\u9608\u503c\u3002\u5b9e\u9a8c\u7ed3\u8bba\u9002\u7528\u4e8e\u8fd9\u4e9b\u5df2\u6267\u884c\u7684\u5904\u7406\u6b65\u9aa4\uff0c\u4e0d\u80fd\u5916\u63a8\u4e3a\u67d0\u4e2a\u7248\u672c\u7684\u6574\u4f53\u7075\u654f\u5ea6\u8bc4\u4ef7\u3002<\/p>\n

\u4e94\u6761\u5019\u9009\u6545\u610f\u5b89\u6392\u4e86\u4e0d\u540c\u95ee\u9898<\/h2>\n

\u8f93\u5165\u8868\u4fdd\u7559\u539f\u59cb\u5206\u88c2\u652f\u6301\u3001\u539f\u59cb\u8de8\u63a5\u652f\u6301\uff0c\u4ee5\u53ca\u4e24\u79cd\u4f30\u8ba1\u652f\u6301\u3002\u524d\u4e24\u5217\u540d\u4e3a JunctionReadCount<\/code> \u548c SpanningFragCount<\/code>\uff0c\u540e\u4e24\u5217\u4e3a est_J<\/code> \u548c est_S<\/code>\u3002\u4f30\u8ba1\u503c\u5728\u672c\u5b9e\u9a8c\u4e2d\u76f4\u63a5\u624b\u5de5\u6307\u5b9a\uff0c\u6ca1\u6709\u901a\u8fc7\u6a21\u578b\u62df\u5408\u5f97\u5230\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n\n
\u5019\u9009\u4ee3\u53f7<\/th>\n\u539f\u59cb\u5206\u88c2\uff0f\u8de8\u63a5<\/th>\n\u4f30\u8ba1\u5206\u88c2\uff0f\u8de8\u63a5<\/th>\n\u7528\u6765\u68c0\u67e5\u7684\u95ee\u9898<\/th>\n<\/tr>\n<\/thead>\n
\u7532<\/td>\n8\uff0f12<\/td>\n8\uff0f12<\/td>\n\u4e24\u79cd\u53e3\u5f84\u4e00\u81f4<\/td>\n<\/tr>\n
\u4e59<\/td>\n8\uff0f12<\/td>\n2.5\uff0f1.5<\/td>\n\u539f\u59cb\u6570\u76f8\u540c\uff0c\u4f30\u8ba1\u6570\u4e0d\u540c<\/td>\n<\/tr>\n
\u4e19<\/td>\n0\uff0f6<\/td>\n0\uff0f6<\/td>\n\u7f3a\u5c11\u5206\u88c2\u8bc1\u636e<\/td>\n<\/tr>\n
\u4e01<\/td>\n1\uff0f0<\/td>\n1\uff0f0<\/td>\n\u652f\u6301\u5f88\u5c11<\/td>\n<\/tr>\n
\u620a<\/td>\n2\uff0f2<\/td>\n1.9992\uff0f1.9992<\/td>\n\u63a5\u8fd1\u9608\u503c\u7684\u820d\u5165<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u7532\u548c\u4e59\u662f\u6700\u91cd\u8981\u7684\u4e00\u5bf9\u5bf9\u7167\u3002\u82e5\u76f4\u63a5\u628a\u539f\u59cb\u4e24\u5217\u76f8\u52a0\uff0c\u4e24\u8005\u90fd\u662f\u4e8c\u5341\u3002\u82e5\u76f8\u52a0\u4f30\u8ba1\u4e24\u5217\uff0c\u5219\u5206\u522b\u662f\u4e8c\u5341\u548c\u56db\u3002\u6587\u7ae0\u4e2d\u7684\u5dee\u5f02\u6765\u81ea\u8fd9\u4e2a\u4eba\u5de5\u8bbe\u5b9a\uff0c\u4e0d\u662f\u8f6f\u4ef6\u81ea\u52a8\u8bc6\u522b\u51fa\u4e86\u54ea\u4e2a\u5019\u9009\u5b58\u5728\u6b67\u4e49\u3002\u8fd9\u6837\u7684\u8bf4\u660e\u5f88\u91cd\u8981\uff0c\u5426\u5219\u8bfb\u8005\u4f1a\u8bef\u4ee5\u4e3a\u6211\u4eec\u5df2\u7ecf\u9a8c\u8bc1\u4e86\u6a21\u578b\u5206\u914d\u8fc7\u7a0b\u3002<\/p>\n

\u4e19\u7528\u4e8e\u68c0\u67e5\u6570\u503c\u7b5b\u9009\u4e0e\u8bc1\u636e\u7c7b\u578b\u662f\u5426\u88ab\u6df7\u6dc6\u3002\u4e00\u4e2a\u4ec5\u68c0\u67e5\u6807\u51c6\u5316\u6570\u503c\u7684\u811a\u672c\uff0c\u5e76\u4e0d\u4f1a\u56e0\u4e3a\u67d0\u7c7b\u8bc1\u636e\u7f3a\u5931\u5c31\u81ea\u52a8\u8865\u4e0a\u53e6\u4e00\u5957\u5224\u65ad\u89c4\u5219\u3002\u620a\u5219\u7528\u4e8e\u68c0\u67e5\u63a5\u8fd1\u8fb9\u754c\u65f6\u8f93\u51fa\u7cbe\u5ea6\u7684\u5f71\u54cd\u3002\u628a\u8fd9\u4e9b\u7279\u6b8a\u8f93\u5165\u653e\u5728\u540c\u4e00\u4e2a\u5c0f\u8868\u91cc\uff0c\u53ef\u4ee5\u6bd4\u53ea\u68c0\u67e5\u4e00\u6761\u666e\u901a\u5019\u9009\u66f4\u5feb\u53d1\u73b0\u4f7f\u7528\u4e0a\u7684\u8bef\u89e3\u3002<\/p>\n

\u5b9e\u9645\u8ba1\u7b97\u8bfb\u7684\u662f\u4f30\u8ba1\u652f\u6301\u5217<\/h2>\n

\u8fd0\u884c\u7684\u7b2c\u4e00\u4e2a\u5b98\u65b9\u811a\u672c\u662f incorporate_FFPM_into_final_report.pl<\/code>\u3002\u5728\u56fa\u5b9a\u7248\u672c\u7684\u6e90\u7801\u4e2d\uff0c\u5b83\u8bfb\u53d6\u4f30\u8ba1\u5206\u88c2\u4e0e\u4f30\u8ba1\u8de8\u63a5\u4e24\u5217\uff0c\u5404\u81ea\u6362\u7b97\u4e3a\u6bcf\u767e\u4e07\u7247\u6bb5\u7684\u6570\u503c\uff0c\u518d\u76f8\u52a0\u5199\u51fa\u3002\u5b83\u5e76\u6ca1\u6709\u76f4\u63a5\u628a\u90a3\u4e24\u5217\u539f\u59cb\u652f\u6301\u6570\u76f8\u52a0\u3002\u5177\u4f53\u884c\u4e3a\u53ef\u6838\u5bf9 \u56fa\u5b9a\u7248\u672c\u8ba1\u7b97\u811a\u672c<\/a>\u3002<\/p>\n

\u5148\u5ffd\u7565\u4fdd\u7559\u5c0f\u6570\u4f4d\u7684\u7ec6\u8282\uff0c\u53ef\u4ee5\u628a\u672c\u5b9e\u9a8c\u4e2d\u7684\u6362\u7b97\u7406\u89e3\u4e3a\uff0c\u4f30\u8ba1\u652f\u6301\u5408\u8ba1\u9664\u4ee5\u8bbe\u5b9a\u7684\u6587\u5e93\u7247\u6bb5\u603b\u6570\uff0c\u518d\u4e58\u4ee5\u4e00\u767e\u4e07\u3002\u5b83\u63cf\u8ff0\u7684\u662f\u76f8\u5bf9\u4e8e\u5206\u6bcd\u7684\u652f\u6301\u5f3a\u5ea6\uff0c\u4e0d\u662f\u6982\u7387\u3002\u6570\u503c\u5927\u4e8e\u4e00\u4e0d\u77db\u76fe\uff0c\u6570\u503c\u4e3a\u96f6\u70b9\u4e8c\u4e5f\u4e0d\u80fd\u8bfb\u6210\u5019\u9009\u4e3a\u771f\u7684\u6982\u7387\u662f\u767e\u5206\u4e4b\u4e8c\u5341\u3002<\/p>\n

\u5728\u771f\u5b9e\u7ed3\u679c\u5ba1\u9605\u4e2d\uff0c\u9996\u5148\u5e94\u8ffd\u95ee\u4f30\u8ba1\u652f\u6301\u4ece\u54ea\u91cc\u6765\uff0c\u5206\u6bcd\u4ece\u54ea\u91cc\u6765\u3002\u53ea\u6709\u628a\u8fd9\u4e24\u4e2a\u6765\u6e90\u89e3\u91ca\u6e05\u695a\uff0c\u6570\u503c\u624d\u5177\u6709\u53ef\u6bd4\u8f83\u7684\u542b\u4e49\u3002\u628a\u5176\u4ed6\u8f6f\u4ef6\u7684\u4e00\u5217\u652f\u6301\u6570\u6539\u540d\u6210\u8fd9\u91cc\u9700\u8981\u7684\u5217\u540d\uff0c\u867d\u7136\u53ef\u80fd\u8ba9\u811a\u672c\u8fd0\u884c\uff0c\u5374\u4e0d\u4ee3\u8868\u4e24\u79cd\u5b9a\u4e49\u76f8\u540c\u3002\u5c24\u5176\u662f\u8de8\u63a5\u7247\u6bb5\u3001\u5355\u7aef\u8bfb\u6bb5\u548c\u5206\u914d\u540e\u7684\u652f\u6301\u6743\u91cd\uff0c\u4e0d\u80fd\u53ea\u56e0\u4e3a\u90fd\u770b\u8d77\u6765\u50cf\u6570\u91cf\u5c31\u6df7\u7528\u3002<\/p>\n

\u5206\u6bcd\u7ffb\u500d\uff0c\u5019\u9009\u540d\u5355\u4e3a\u4ec0\u4e48\u7f29\u77ed<\/h2>\n

\u6211\u4eec\u7ed9\u540c\u4e00\u5f20\u5019\u9009\u8868\u5206\u522b\u4f20\u5165\u4e24\u5343\u4e07\u548c\u56db\u5343\u4e07\u7684\u5206\u6bcd\u3002\u8fd9\u662f\u4e24\u79cd\u4eba\u4e3a\u8bbe\u5b9a\u7684\u7b97\u672f\u60c5\u5883\uff0c\u5e76\u6ca1\u6709\u771f\u7684\u751f\u6210\u6216\u6d4b\u5e8f\u8fd9\u4e48\u591a\u7247\u6bb5\u3002\u5206\u6bcd\u53d8\u5316\u65f6\uff0c\u5206\u5b50\u3001\u5019\u9009\u540d\u79f0\u548c\u4f30\u8ba1\u503c\u5168\u90e8\u4e0d\u53d8\uff0c\u56e0\u6b64\u53ef\u4ee5\u5355\u72ec\u89c2\u5bdf\u6807\u51c6\u5316\u7684\u5f71\u54cd\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n\n
\u5019\u9009\u4ee3\u53f7<\/th>\n\u5206\u6bcd\u4e24\u5343\u4e07<\/th>\n\u5206\u6bcd\u56db\u5343\u4e07<\/th>\n\u9608\u503c\u96f6\u70b9\u4e8c\u4e0b\u7684\u53d8\u5316<\/th>\n<\/tr>\n<\/thead>\n
\u7532<\/td>\n1<\/td>\n0.5<\/td>\n\u4e24\u6b21\u4fdd\u7559<\/td>\n<\/tr>\n
\u4e59<\/td>\n0.2<\/td>\n0.1<\/td>\n\u7b2c\u4e8c\u6b21\u4e0d\u4fdd\u7559<\/td>\n<\/tr>\n
\u4e19<\/td>\n0.3<\/td>\n0.15<\/td>\n\u7b2c\u4e8c\u6b21\u4e0d\u4fdd\u7559<\/td>\n<\/tr>\n
\u4e01<\/td>\n0.05<\/td>\n0.025<\/td>\n\u4e24\u6b21\u90fd\u4e0d\u4fdd\u7559<\/td>\n<\/tr>\n
\u620a<\/td>\n0.2<\/td>\n0.1<\/td>\n\u7b2c\u4e8c\u6b21\u4e0d\u4fdd\u7559<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u76f8\u540c\u8ba1\u6570\u5728\u66f4\u5927\u7684\u6587\u5e93\u5206\u6bcd\u4e2d\u6240\u5360\u6bd4\u4f8b\u66f4\u4f4e\uff0c\u56e0\u6b64\u8fd9\u7ec4\u7ed3\u679c\u7b26\u5408\u6362\u7b97\u5173\u7cfb\u3002\u4f46\u4e0d\u80fd\u636e\u6b64\u8bf4\u589e\u52a0\u6d4b\u5e8f\u6df1\u5ea6\u4f1a\u964d\u4f4e\u771f\u5b9e\u68c0\u51fa\u7387\u3002\u672c\u5b9e\u9a8c\u6ca1\u6709\u8ba9\u8bfb\u6bb5\u548c\u5206\u6bcd\u4e00\u8d77\u589e\u52a0\uff0c\u4e5f\u6ca1\u6709\u6a21\u62df\u53d6\u6837\u8fc7\u7a0b\u3002\u5b83\u53ea\u8bf4\u660e\uff0c\u5f53\u6574\u7406\u4eba\u5458\u9009\u9519\u5206\u6bcd\u6216\u8005\u6df7\u7528\u4e0d\u540c\u53e3\u5f84\u7684\u5206\u6bcd\u65f6\uff0c\u5019\u9009\u662f\u5426\u8de8\u8fc7\u95e8\u69db\u4f1a\u6539\u53d8\u3002<\/p>\n

\u4e00\u4e2a\u5e38\u89c1\u7684\u6838\u67e5\u70b9\u662f\u53cc\u7aef\u6570\u636e\u7684\u5355\u4f4d\u3002\u82e5\u4e00\u4e2a\u7247\u6bb5\u5bf9\u5e94\u4e24\u7aef\u8bfb\u6bb5\uff0c\u628a\u4e24\u7aef\u6587\u4ef6\u4e2d\u7684\u8bfb\u6bb5\u6570\u7b80\u5355\u76f8\u52a0\u4f5c\u4e3a\u7247\u6bb5\u6570\uff0c\u5c31\u53ef\u80fd\u628a\u5206\u6bcd\u6269\u5927\u4e00\u500d\u3002\u5b9e\u9645\u6d41\u7a0b\u8be5\u4f7f\u7528\u54ea\u4efd\u7edf\u8ba1\uff0c\u5e94\u6309\u8be5\u7248\u672c\u5de5\u4f5c\u6d41\u7a0b\u548c\u6587\u4ef6\u5143\u6570\u636e\u786e\u5b9a\uff0c\u4e0d\u80fd\u4e3a\u4e86\u590d\u73b0\u67d0\u4e2a\u5e0c\u671b\u770b\u5230\u7684\u6570\u503c\uff0c\u4e34\u65f6\u9009\u62e9\u770b\u8d77\u6765\u66f4\u5408\u9002\u7684\u5206\u6bcd\u3002<\/p>\n

\u8de8\u6837\u672c\u6bd4\u8f83\u8fd8\u9700\u8981\u786e\u8ba4\u524d\u5904\u7406\u662f\u5426\u4e00\u81f4\u3002\u4e00\u4e2a\u6837\u672c\u7528\u539f\u59cb\u8f93\u5165\u603b\u91cf\uff0c\u53e6\u4e00\u4e2a\u6837\u672c\u7528\u8fc7\u6ee4\u540e\u7684\u603b\u91cf\uff0c\u4e24\u8005\u867d\u7136\u90fd\u53eb\u6bcf\u767e\u4e07\uff0c\u6240\u4ee3\u8868\u7684\u80cc\u666f\u5df2\u7ecf\u4e0d\u540c\u3002\u5efa\u8bae\u5c06\u5206\u6bcd\u7684\u6570\u503c\u3001\u5355\u4f4d\u3001\u6765\u6e90\u6587\u4ef6\u548c\u7edf\u8ba1\u9636\u6bb5\u5355\u72ec\u4fdd\u5b58\u3002\u8fd9\u6837\u53d1\u73b0\u7ed3\u679c\u4e0d\u4e00\u81f4\u65f6\uff0c\u53ef\u4ee5\u5148\u68c0\u67e5\u53e3\u5f84\uff0c\u800c\u4e0d\u662f\u7acb\u5373\u5f52\u56e0\u4e8e\u751f\u7269\u5dee\u5f02\u3002<\/p>\n

\u901a\u8fc7\u6570\u503c\u95e8\u69db\u4e0d\u4ee3\u8868\u901a\u8fc7\u5b8c\u6574\u68c0\u6d4b<\/h2>\n

\u7b2c\u4e8c\u4e2a\u5b9e\u9645\u6267\u884c\u7684\u811a\u672c\u4e3a filter_by_min_FFPM.pl<\/code>\u3002\u6211\u4eec\u4eba\u4e3a\u9009\u62e9\u96f6\u70b9\u4e8c\u4f5c\u4e3a\u6f14\u793a\u9608\u503c\uff0c\u5b83\u4e0d\u662f\u672c\u6587\u63a8\u8350\u7684\u901a\u7528\u7b5b\u9009\u6807\u51c6\u3002\u56fa\u5b9a\u7248\u672c\u4f7f\u7528\u5927\u4e8e\u6216\u7b49\u4e8e\u8fdb\u884c\u6bd4\u8f83\uff0c\u56e0\u6b64\u6070\u597d\u7b49\u4e8e\u9608\u503c\u7684\u4e59\u4f1a\u4fdd\u7559\u3002\u5206\u6bcd\u4e3a\u4e24\u5343\u4e07\u65f6\u7559\u4e0b\u56db\u6761\uff0c\u5206\u6bcd\u4e3a\u56db\u5343\u4e07\u65f6\u53ea\u7559\u4e0b\u7532\u3002\u903b\u8f91\u89c1 \u56fa\u5b9a\u7248\u672c\u8fc7\u6ee4\u811a\u672c<\/a>\u3002<\/p>\n

\u4e19\u6ca1\u6709\u5206\u88c2\u652f\u6301\uff0c\u4f46\u5728\u7b2c\u4e00\u79cd\u60c5\u5883\u4e2d\u6570\u503c\u4e3a\u96f6\u70b9\u4e09\uff0c\u4ecd\u7136\u901a\u8fc7\u8fd9\u4e2a\u811a\u672c\u3002\u8fd9\u5e76\u4e0d\u8bf4\u660e\u5b83\u4f1a\u901a\u8fc7 STAR-Fusion \u5b8c\u6574\u6d41\u7a0b\u3002\u539f\u56e0\u5f88\u7b80\u5355\uff0c\u6211\u4eec\u53ea\u6267\u884c\u4e86\u4e00\u4e2a\u6570\u503c\u7b5b\u9009\uff0c\u6ca1\u6709\u6267\u884c\u5176\u4ed6\u6709\u5173\u5019\u9009\u7ed3\u6784\u548c\u8bc1\u636e\u7684\u6b65\u9aa4\u3002\u7ed3\u679c\u6587\u4ef6\u91cc\u7684\u901a\u8fc7\uff0c\u53ea\u80fd\u5728\u8fd9\u4e2a\u5177\u4f53\u6b65\u9aa4\u7684\u8303\u56f4\u5185\u89e3\u91ca\u3002<\/p>\n

\u7531\u6b64\u53ef\u4ee5\u6574\u7406\u51fa\u4e00\u4e2a\u66f4\u7a33\u59a5\u7684\u5ba1\u9605\u987a\u5e8f\u3002\u5148\u786e\u8ba4\u5019\u9009\u6765\u81ea\u54ea\u4e2a\u6d41\u7a0b\u9636\u6bb5\uff0c\u518d\u68c0\u67e5\u5206\u88c2\u548c\u8de8\u63a5\u8bc1\u636e\u5206\u522b\u662f\u4ec0\u4e48\uff0c\u7136\u540e\u6838\u5bf9\u6807\u51c6\u5316\u6570\u503c\uff0c\u6700\u540e\u624d\u8ba8\u8bba\u7b5b\u9009\u9608\u503c\u3002\u82e5\u987a\u5e8f\u53cd\u8fc7\u6765\uff0c\u5148\u7528\u4e00\u4e2a\u9608\u503c\u5220\u8868\uff0c\u518d\u53bb\u89e3\u91ca\u5269\u4f59\u5019\u9009\uff0c\u5f88\u5bb9\u6613\u628a\u5c1a\u672a\u9a8c\u8bc1\u7684\u8bc1\u636e\u8981\u6c42\u5f53\u6210\u7a0b\u5e8f\u5df2\u7ecf\u66ff\u81ea\u5df1\u68c0\u67e5\u8fc7\u3002<\/p>\n

\u63a5\u8fd1\u8fb9\u754c\u65f6\uff0c\u4fdd\u7559\u4f4d\u6570\u4f1a\u53c2\u4e0e\u51b3\u5b9a<\/h2>\n

\u620a\u7684\u4f30\u8ba1\u652f\u6301\u5408\u8ba1\u4e3a\u4e09\u70b9\u4e5d\u4e5d\u516b\u56db\u3002\u7528\u4e24\u5343\u4e07\u4f5c\u4e3a\u5206\u6bcd\uff0c\u672a\u820d\u5165\u7684\u5408\u8ba1\u6570\u503c\u4e3a\u96f6\u70b9\u4e00\u4e5d\u4e5d\u4e5d\u4e8c\uff0c\u7565\u5c0f\u4e8e\u6f14\u793a\u95e8\u69db\u3002\u4f46\u662f\u5b9e\u9645\u811a\u672c\u5148\u5206\u522b\u8ba1\u7b97\u4e24\u90e8\u5206\uff0c\u5e76\u5c06\u6bcf\u90e8\u5206\u4fdd\u7559\u56db\u4f4d\u5c0f\u6570\u3002\u4e24\u90e8\u5206\u5404\u81ea\u6210\u4e3a\u96f6\u70b9\u4e00\uff0c\u76f8\u52a0\u5199\u51fa\u7684\u7ed3\u679c\u5c31\u662f\u96f6\u70b9\u4e8c\uff0c\u968f\u540e\u901a\u8fc7\u5305\u542b\u7b49\u53f7\u7684\u7b5b\u9009\u3002<\/p>\n

\u8fd9\u4e0d\u662f\u6211\u4eec\u5047\u8bbe\u7684\u6d6e\u70b9\u98ce\u9669\uff0c\u800c\u662f\u672c\u6b21\u8fd0\u884c\u786e\u5b9e\u5f97\u5230\u7684\u8f93\u51fa\u3002\u9700\u8981\u6ce8\u610f\uff0c\u5b9e\u9a8c\u9a8c\u8bc1\u7684\u662f\u6307\u5b9a\u811a\u672c\u548c\u6307\u5b9a\u53c2\u6570\u7684\u7ec4\u5408\uff0c\u4e0d\u80fd\u76f4\u63a5\u63a8\u5e7f\u6210\u6574\u4e2a\u8f6f\u4ef6\u6240\u6709\u6b65\u9aa4\u90fd\u7528\u76f8\u540c\u7cbe\u5ea6\u3002\u9605\u8bfb\u7ed3\u679c\u65f6\uff0c\u5e94\u628a\u663e\u793a\u6570\u503c\u3001\u5185\u90e8\u6570\u503c\u548c\u4e0b\u6e38\u5b9e\u9645\u8bfb\u53d6\u7684\u6570\u503c\u5206\u5f00\uff0c\u7279\u522b\u662f\u5c06\u6587\u672c\u8868\u683c\u518d\u6b21\u8f93\u5165\u5176\u4ed6\u7a0b\u5e8f\u7684\u65f6\u5019\u3002<\/p>\n

\u5904\u7406\u8fb9\u754c\u5019\u9009\uff0c\u4e0d\u5b9c\u901a\u8fc7\u6539\u53d8\u5c0f\u6570\u4f4d\u8ba9\u5b83\u5f97\u5230\u9884\u60f3\u4e2d\u7684\u5f52\u7c7b\u3002\u66f4\u6709\u7528\u7684\u505a\u6cd5\u662f\u4fdd\u7559\u4e0a\u6e38\u7cbe\u5ea6\u3001\u8bb0\u5f55\u6bcf\u4e00\u6b65\u8f6c\u6362\uff0c\u5e76\u628a\u975e\u5e38\u63a5\u8fd1\u95e8\u69db\u7684\u6761\u76ee\u4f5c\u4e3a\u8fb9\u754c\u6848\u4f8b\u67e5\u770b\u3002\u5982\u679c\u91cd\u7b97\u516c\u5f0f\u4e0e\u5bfc\u51fa\u7684\u7ed3\u679c\u76f8\u5dee\u5f88\u5c0f\uff0c\u5e94\u5148\u68c0\u67e5\u662f\u5206\u6b65\u820d\u5165\u8fd8\u662f\u6700\u7ec8\u820d\u5165\uff0c\u518d\u5224\u65ad\u662f\u5426\u6709\u771f\u6b63\u7684\u8ba1\u6570\u9519\u8bef\u3002<\/p>\n

\u4e00\u4e2a\u5b9e\u9645\u8e29\u5230\u7684\u683c\u5f0f\u95ee\u9898<\/h2>\n

\u9996\u6b21\u751f\u6210\u8f93\u5165\u65f6\uff0c\u6211\u4eec\u4f7f\u7528\u4e86\u5e26\u56de\u8f66\u6362\u884c\u7684\u5236\u8868\u7b26\u6587\u4ef6\u3002\u5b98\u65b9\u8bfb\u53d6\u6a21\u5757\u6ca1\u6709\u53bb\u6389\u672b\u5217\u8868\u5934\u4e2d\u7684\u56de\u8f66\uff0c\u968f\u540e\u53c8\u7ecf\u8fc7\u672c\u9a71\u52a8\u811a\u672c\u7684\u6587\u672c\u6355\u83b7\u4e0e\u6362\u884c\u8f6c\u6362\uff0c\u8ba1\u7b97\u8f93\u51fa\u51fa\u73b0\u4e86\u5f02\u5e38\u5206\u884c\u3002\u8ba1\u7b97\u6b65\u9aa4\u867d\u7136\u8fd4\u56de\u6210\u529f\uff0c\u540e\u7eed\u8fc7\u6ee4\u5374\u62a5\u51fa\u5b57\u6bb5\u6570\u91cf\u4e0d\u7b26\u3002\u6539\u4e3a\u660e\u786e\u4f7f\u7528\u5355\u4e00\u6362\u884c\u7b26\u540e\uff0c\u4e24\u7ec4\u8ba1\u7b97\u548c\u8fc7\u6ee4\u5747\u901a\u8fc7\u3002<\/p>\n

\u8fd9\u91cc\u4e0d\u80fd\u7b80\u5355\u5199\u6210\u8f6f\u4ef6\u4e0d\u652f\u6301\u6240\u6709 Windows \u6587\u4ef6\uff0c\u56e0\u4e3a\u6211\u4eec\u590d\u73b0\u7684\u662f\u7279\u5b9a\u8f93\u5165\u52a0\u4e0a\u7279\u5b9a\u6355\u83b7\u65b9\u5f0f\u7684\u5b8c\u6574\u94fe\u6761\u3002\u66f4\u51c6\u786e\u7684\u7ecf\u9a8c\u662f\uff0c\u8de8\u7cfb\u7edf\u751f\u6210\u8868\u683c\u65f6\uff0c\u5e94\u68c0\u67e5\u4e0d\u53ef\u89c1\u5b57\u7b26\u3001\u672b\u5217\u8868\u5934\u4ee5\u53ca\u6807\u51c6\u9519\u8bef\u8f93\u51fa\u3002\u547d\u4ee4\u8fd4\u56de\u6210\u529f\u4ec5\u4ec5\u662f\u4e00\u4e2a\u68c0\u67e5\u70b9\uff0c\u4e0d\u80fd\u66ff\u4ee3\u5bf9\u8f93\u51fa\u5217\u6570\u548c\u5173\u952e\u5b57\u6bb5\u7684\u6838\u9a8c\u3002<\/p>\n

\u9a71\u52a8\u811a\u672c\u4e2d\u73b0\u5728\u660e\u786e\u6307\u5b9a\u6362\u884c\u65b9\u5f0f\uff0c\u5e76\u4fdd\u7559\u4e86\u8fd9\u4e2a\u5931\u8d25\u60c5\u5883\u7684\u8bca\u65ad\u8bb0\u5f55\u3002\u8bfb\u8005\u53ef\u4ee5\u91cd\u73b0\u5dee\u5f02\uff0c\u4e0d\u5fc5\u9760\u622a\u56fe\u731c\u6d4b\u8f93\u5165\u662f\u5426\u76f8\u540c\u3002\u5904\u7406\u6b63\u5f0f\u7ed3\u679c\u65f6\uff0c\u4fee\u590d\u683c\u5f0f\u5e94\u5f53\u4ea7\u751f\u4e00\u4efd\u65b0\u6587\u4ef6\uff0c\u5e76\u4fdd\u7559\u539f\u59cb\u6587\u4ef6\uff0c\u907f\u514d\u628a\u8f93\u5165\u4fee\u590d\u548c\u751f\u7269\u5b66\u8fc7\u6ee4\u6df7\u6210\u4e00\u4e2a\u4e0d\u53ef\u8ffd\u6eaf\u7684\u64cd\u4f5c\u3002<\/p>\n

with open("candidates.tsv", "w", newline="") as f:\r\n    writer = csv.writer(f, delimiter="\\t", lineterminator="\\n")\r\n    writer.writerow(columns)\r\n    writer.writerows(rows)\r\n<\/code><\/pre>\n
\u600e\u6837\u590d\u73b0\u8fd9\u6b21\u7ed3\u679c\u5904\u7406<\/h2>\n
\u5b8c\u6574\u811a\u672c\u548c\u5b9e\u9645\u7ed3\u679c\u89c1 \u672c\u7ec4\u5b9e\u8dd1\u8d44\u6599<\/a>\u3002\u7a0b\u5e8f\u4e0b\u8f7d\u56fa\u5b9a\u7248\u672c\u7684\u5b98\u65b9\u6e90\u7801\uff0c\u6821\u9a8c\u538b\u7f29\u5305\uff0c\u5b89\u88c5\u672c\u5b9e\u9a8c\u9700\u8981\u7684\u4e24\u4e2a\u811a\u672c\u53ca\u5176\u8bfb\u53d6\u6a21\u5757\uff0c\u5728\u56fa\u5b9a\u8fd0\u884c\u955c\u50cf\u4e2d\u6267\u884c\u3002\u5b83\u6ca1\u6709\u5b89\u88c5\u5e76\u8fd0\u884c\u5b8c\u6574\u7684\u4eba\u7c7b\u878d\u5408\u53d1\u73b0\u5de5\u4f5c\u6d41\uff0c\u538b\u7f29\u5305\u4e2d\u7684\u5b9e\u9a8c\u8303\u56f4\u4e5f\u5bf9\u6b64\u4f5c\u4e86\u6807\u6ce8\u3002<\/p>\n
\u4e0b\u9762\u662f\u6838\u5fc3\u5904\u7406\u6b65\u9aa4\u3002\u8f93\u5165\u4e2d\u7684\u4f30\u8ba1\u652f\u6301\u5217\u7531\u7ec3\u4e60\u9a71\u52a8\u811a\u672c\u521b\u5efa\uff0c\u4e0d\u80fd\u7701\u7565\u540e\u76f4\u63a5\u5957\u5728\u4efb\u610f\u5019\u9009\u8868\u4e0a\u3002\u8f93\u51fa\u6587\u4ef6\u540d\u53ea\u662f\u672c\u6b21\u7ec3\u4e60\u7684\u7ea6\u5b9a\uff0c\u4f7f\u7528\u81ea\u5df1\u7684\u6570\u636e\u65f6\u5e94\u5f53\u5148\u68c0\u67e5\u5217\u540d\u662f\u5426\u4e0e\u811a\u672c\u8981\u6c42\u4e00\u81f4\u3002<\/p>\n
perl STAR-Fusion\/util\/incorporate_FFPM_into_final_report.pl \\\r\n  fictional_candidates.tsv 20000000 > denom_20000000.tsv\r\n\r\nperl STAR-Fusion\/util\/filter_by_min_FFPM.pl \\\r\n  denom_20000000.tsv 0.2\r\n<\/code><\/pre>\n
\u590d\u73b0\u4e4b\u540e\uff0c\u53ef\u4ee5\u5c1d\u8bd5\u53ea\u4fee\u6539\u4e00\u9879\u8f93\u5165\u3002\u4f8b\u5982\u4fdd\u6301\u4f30\u8ba1\u652f\u6301\u4e0d\u53d8\uff0c\u53ea\u6539\u539f\u59cb\u652f\u6301\u5217\uff0c\u518d\u67e5\u770b\u6807\u51c6\u5316\u6570\u503c\u662f\u5426\u53d8\u5316\u3002\u4e5f\u53ef\u4ee5\u56fa\u5b9a\u5206\u6bcd\uff0c\u5c06\u4e00\u4e2a\u5019\u9009\u653e\u5728\u95e8\u69db\u7684\u4e24\u4fa7\uff0c\u68c0\u67e5\u6bd4\u8f83\u7b26\u53f7\u3002\u6bcf\u6b21\u53ea\u6539\u4e00\u4e2a\u56e0\u7d20\uff0c\u624d\u80fd\u660e\u786e\u7ed3\u679c\u53d8\u5316\u7531\u54ea\u91cc\u4ea7\u751f\u3002\u8fd9\u7c7b\u7ec3\u4e60\u9002\u5408\u68c0\u67e5\u81ea\u7f16\u5408\u5e76\u8868\u683c\u7684\u7a0b\u5e8f\uff0c\u5c24\u5176\u662f\u66ff\u6362\u5217\u540d\u548c\u91cd\u65b0\u6807\u51c6\u5316\u7684\u6b65\u9aa4\u3002<\/p>\n
\u5b9e\u9645\u6574\u5408\u591a\u4e2a\u6837\u672c\u65f6\uff0c\u8fd8\u5e94\u628a\u5019\u9009\u7684\u8eab\u4efd\u5b9a\u4e49\u6e05\u695a\u3002\u4ec5\u51ed\u4e24\u4e2a\u57fa\u56e0\u540d\u79f0\u5408\u5e76\uff0c\u53ef\u80fd\u628a\u4e0d\u540c\u65ad\u70b9\u6216\u4e0d\u540c\u8fde\u63a5\u5f62\u5f0f\u538b\u6210\u540c\u4e00\u884c\u3002\u672c\u6587\u6ca1\u6709\u6267\u884c\u65ad\u70b9\u5408\u5e76\uff0c\u56e0\u6b64\u4e0d\u63d0\u4f9b\u4e00\u4e2a\u672a\u7ecf\u9a8c\u8bc1\u7684\u5408\u5e76\u8ddd\u79bb\u3002\u5bf9\u4e8e\u9700\u8981\u6c47\u603b\u7684\u9879\u76ee\uff0c\u5e94\u5148\u786e\u5b9a\u62a5\u544a\u5bf9\u8c61\u662f\u57fa\u56e0\u5bf9\u8fd8\u662f\u5177\u4f53\u8fde\u63a5\uff0c\u518d\u9009\u62e9\u76f8\u5e94\u7684\u952e\u503c\uff0c\u4fdd\u7559\u4e0d\u80fd\u76f4\u63a5\u5408\u5e76\u7684\u8bc1\u636e\u3002<\/p>\n
\u4f18\u70b9\u3001\u5c40\u9650\u4e0e\u9009\u7528\u7ecf\u9a8c<\/h2>\n
STAR-Fusion \u7684\u5b9e\u7528\u4ef7\u503c\u5728\u4e8e\u6709\u660e\u786e\u7684\u5de5\u4f5c\u6d41\u7a0b\u548c\u53ef\u68c0\u67e5\u7684\u4e2d\u95f4\u5904\u7406\u6b65\u9aa4\u3002\u516c\u5f00\u7684\u811a\u672c\u4f7f\u8ba1\u6570\u6765\u6e90\u3001\u6807\u51c6\u5316\u548c\u9608\u503c\u884c\u4e3a\u80fd\u591f\u88ab\u8ffd\u8e2a\u3002\u672c\u6b21\u53d1\u73b0\u539f\u59cb\u6570\u4e0e\u4f30\u8ba1\u6570\u4e0d\u80fd\u4e92\u6362\uff0c\u6b63\u662f\u901a\u8fc7\u9605\u8bfb\u56fa\u5b9a\u7248\u672c\u4ee3\u7801\u5e76\u8fd0\u884c\u5bf9\u7167\u5f97\u5230\u7684\uff0c\u800c\u4e0d\u662f\u51ed\u5b57\u6bb5\u540d\u79f0\u731c\u6d4b\u3002<\/p>\n
\u5c40\u9650\u540c\u6837\u9700\u8981\u8bf4\u6e05\u3002\u6700\u7ec8\u8868\u683c\u4f1a\u538b\u7f29\u8bb8\u591a\u4e0a\u6e38\u4fe1\u606f\uff0c\u6807\u51c6\u5316\u6570\u5b57\u4e0d\u80fd\u8868\u8fbe\u5168\u90e8\u8bc1\u636e\u7ed3\u6784\uff0c\u5355\u4e2a\u9608\u503c\u4e5f\u4e0d\u80fd\u5904\u7406\u6240\u6709\u5f02\u5e38\u3002\u5b8c\u6574\u68c0\u6d4b\u9700\u8981\u9002\u914d\u7684\u53c2\u8003\u8d44\u6e90\u548c\u8f93\u5165\u6761\u4ef6\uff0c\u540e\u5904\u7406\u7ec3\u4e60\u5e76\u672a\u9a8c\u8bc1\u8fd9\u4e9b\u90e8\u5206\u3002\u65b9\u6cd5\u6574\u4f53\u8868\u73b0\u5e94\u53c2\u8003\u539f\u59cb\u7814\u7a76\u53ca\u4e0e\u5b9e\u9645\u6570\u636e\u76f8\u7b26\u7684\u9a8c\u8bc1\u8bbe\u8ba1\uff0c\u800c\u4e0d\u80fd\u7528\u672c\u7bc7\u7684\u4e94\u6761\u4eba\u5de5\u5019\u9009\u4ee3\u66ff\uff0c\u76f8\u5173\u7814\u7a76\u89c1 STAR-Fusion \u65b9\u6cd5\u8bba\u6587<\/a>\u3002<\/p>\n
\u771f\u6b63\u503c\u5f97\u4fdd\u7559\u7684\u5de5\u4f5c\u4e60\u60ef\uff0c\u662f\u628a\u6bcf\u4e2a\u6570\u503c\u8fd8\u539f\u6210\u5206\u5b50\u3001\u5206\u6bcd\u3001\u7cbe\u5ea6\u548c\u6765\u6e90\u3002\u9047\u5230\u5019\u9009\u540d\u5355\u53d8\u5316\u65f6\uff0c\u5148\u68c0\u67e5\u8fd9\u56db\u4ef6\u4e8b\uff0c\u5f80\u5f80\u6bd4\u53cd\u590d\u8c03\u9608\u503c\u66f4\u6709\u6548\u3002\u4e00\u4e2a\u80fd\u591f\u8bf4\u660e\u4e3a\u4ec0\u4e48\u7559\u4e0b\u6216\u5220\u9664\u67d0\u6761\u5019\u9009\u7684\u6d41\u7a0b\uff0c\u4e5f\u66f4\u5bb9\u6613\u88ab\u5176\u4ed6\u5206\u6790\u4eba\u5458\u590d\u6838\u548c\u7ee7\u7eed\u4f7f\u7528\u3002<\/p>\n
\u5f53\u5df2\u6838\u5bf9\u7684\u878d\u5408\u5206\u6790\u6d41\u7a0b\u9700\u8981\u6269\u5c55\u5230\u66f4\u591a\u6837\u672c\u65f6\uff0c\u53ef\u7ed3\u5408\u6570\u636e\u89c4\u6a21\u4e86\u89e3 \u4e91\u751f\u4fe1\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u670d\u52a1\u5668\u670d\u52a1<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
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