{"id":73198,"date":"2026-11-15T14:50:22","date_gmt":"2026-11-15T06:50:22","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73198"},"modified":"2026-11-15T14:50:22","modified_gmt":"2026-11-15T06:50:22","slug":"macs3-frag-count-barcode-bedpe-peak-coordinate-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/macs3-frag-count-barcode-bedpe-peak-coordinate-practice\/","title":{"rendered":"MACS3 \u5b9e\u8dd1\u590d\u76d8\uff0c255 \u884c\u7247\u6bb5\u6587\u4ef6\u4e3a\u4ec0\u4e48\u4f1a\u6309 500 \u4e2a\u7247\u6bb5\u8c03\u7528\u5cf0"},"content":{"rendered":"
\n

\u540c\u4e00\u4efd\u7247\u6bb5\u6587\u4ef6\uff0c\u6587\u4ef6\u884c\u6570\u6ca1\u6709\u53d8\uff0cMACS3 \u65e5\u5fd7\u4e2d\u7684\u7247\u6bb5\u603b\u91cf\u5374\u53ef\u4ee5\u4e0d\u540c\uff0c\u6700\u7ec8\u5cf0\u5217\u8868\u4e5f\u4f1a\u6539\u53d8\u3002\u539f\u56e0\u4e0d\u4e00\u5b9a\u662f\u7a0b\u5e8f\u91cd\u590d\u8bfb\u53d6\u4e86\u8f93\u5165\uff0c\u800c\u53ef\u80fd\u662f\u6307\u5b9a\u683c\u5f0f\u540e\u662f\u5426\u4f7f\u7528\u6b21\u6570\u5217\u3002\u672c\u6b21\u7528\u4e00\u4efd\u4e94\u5217\u4eba\u5de5\u7247\u6bb5\u6587\u4ef6\uff0c\u5b9e\u9645\u8fd0\u884c\u56db\u7ec4\u5cf0\u8c03\u7528\uff0c\u628a\u6587\u4ef6\u884c\u6570\u3001\u6b21\u6570\u8d21\u732e\u3001\u6761\u7801\u7b5b\u9009\u548c\u8f93\u51fa\u5750\u6807\u9010\u9879\u6838\u5bf9\u3002<\/p>\n

\u8fd9\u7bc7\u6587\u7ae0\u9002\u5408\u9700\u8981\u6574\u7406\u7247\u6bb5\u6587\u4ef6\u3001\u6309\u7ec6\u80de\u96c6\u5408\u8c03\u7528\u5cf0\u6216\u590d\u67e5\u5cf0\u8868\u7684\u8bfb\u8005\u3002\u5b9e\u9a8c\u4f7f\u7528\u5b8c\u5168\u865a\u6784\u7684\u5750\u6807\u548c\u6761\u7801\uff0c\u6ca1\u6709\u771f\u5b9e\u7ec6\u80de\u3001\u6bd4\u5bf9\u6216\u6d4b\u5e8f\u8d28\u91cf\u4fe1\u606f\u3002\u5b83\u5c55\u793a\u683c\u5f0f\u4e0e\u5904\u7406\u89c4\u5219\u5982\u4f55\u5f71\u54cd\u7ed3\u679c\uff0c\u4e0d\u8bc4\u4ef7\u771f\u5b9e\u6837\u672c\u7684\u68c0\u51fa\u7387\uff0c\u4e5f\u4e0d\u628a\u4eba\u5de5\u5cf0\u5f53\u4f5c\u751f\u7269\u5b66\u53d1\u73b0\u3002<\/p>\n

\"\u6587\u4ef6\u884c\u6570\u4e0d\u7b49\u4e8e\u7247\u6bb5\u6b21\u6570\u3002\u540c\u4e00\u4efd\u4eba\u5de5\u6587\u4ef6
\u540c\u4e00\u4efd\u4eba\u5de5\u6587\u4ef6 \u00b7 \u683c\u5f0f\u3001\u6b21\u6570\u4e0e\u6761\u7801\u7684\u56db\u7ec4\u5bf9\u7167<\/figcaption><\/figure>\n

\u5148\u786e\u8ba4\u8f6f\u4ef6\u628a\u4e00\u884c\u7406\u89e3\u6210\u4ec0\u4e48<\/h2>\n

\u672c\u6b21\u5b89\u88c5\u5e76\u8fd0\u884c MACS3 3.1.0\uff0c\u660e\u786e\u6307\u5b9a\u8f93\u5165\u683c\u5f0f\u3002\u4e94\u5217\u6587\u4ef6\u4f9d\u6b21\u5305\u542b\u67d3\u8272\u4f53\u3001\u8d77\u70b9\u3001\u7ec8\u70b9\u3001\u6761\u7801\u548c\u6b21\u6570\u3002\u4f7f\u7528 FRAG \u683c\u5f0f\u65f6\uff0c\u7a0b\u5e8f\u4f1a\u8bfb\u53d6\u6761\u7801\u4e0e\u6b21\u6570\uff0c\u800c\u7b80\u5316\u7684 BEDPE \u6a21\u5f0f\u4e3b\u8981\u6309\u7247\u6bb5\u533a\u95f4\u8bfb\u53d6\u3002\u5bf9\u4e8e\u8fd9\u79cd\u6709\u989d\u5916\u4fe1\u606f\u7684\u8f93\u5165\uff0c\u6587\u4ef6\u540e\u7f00\u76f8\u540c\u5e76\u4e0d\u4fdd\u8bc1\u89e3\u91ca\u65b9\u5f0f\u76f8\u540c\u3002<\/p>\n

\u8fd8\u8981\u6ce8\u610f\uff0cMACS3 \u6240\u8bf4\u7684\u7b80\u5316 BEDPE\uff0c\u4e0e\u53e6\u4e00\u4e9b\u533a\u95f4\u5de5\u5177\u4f7f\u7528\u7684\u53cc\u533a\u95f4\u683c\u5f0f\u5e76\u4e0d\u662f\u540c\u4e00\u5957\u5b57\u6bb5\u5b9a\u4e49\u3002\u4e0d\u80fd\u770b\u5230\u540d\u5b57\u76f8\u540c\u5c31\u4e0d\u68c0\u67e5\u5217\u3002\u5b98\u65b9\u683c\u5f0f\u53ca\u9009\u9879\u8981\u6c42\u89c1 MACS3 \u5cf0\u8c03\u7528\u8bf4\u660e<\/a>\u3002\u672c\u5b9e\u9a8c\u901a\u8fc7\u8fd0\u884c\u65e5\u5fd7\u9a8c\u8bc1\u5b9e\u9645\u8bfb\u53d6\u6570\u91cf\uff0c\u800c\u4e0d\u662f\u4ec5\u6839\u636e\u6587\u6863\u63a8\u7b97\u9884\u671f\u503c\u3002<\/p>\n

\u8fd9\u79cd\u68c0\u67e5\u5e94\u8be5\u53d1\u751f\u5728\u5206\u6790\u5f00\u59cb\u65f6\u3002\u5148\u62bd\u770b\u6587\u4ef6\u5934\u3001\u786e\u8ba4\u5217\u6570\uff0c\u518d\u5c06\u65e5\u5fd7\u4e2d\u7684\u603b\u91cf\u4e0e\u81ea\u5df1\u6309\u5b9a\u4e49\u8ba1\u7b97\u7684\u6570\u91cf\u5bf9\u7167\u3002\u82e5\u4e24\u8005\u4e0d\u540c\uff0c\u5e94\u5148\u89e3\u51b3\u8ba1\u6570\u53e3\u5f84\u95ee\u9898\uff0c\u4e0d\u8981\u7acb\u5373\u8c03\u6574\u663e\u8457\u6027\u9608\u503c\u3002\u5426\u5219\u53ef\u80fd\u5728\u9519\u8bef\u7406\u89e3\u7684\u8f93\u5165\u4e0a\uff0c\u9010\u6b65\u8c03\u51fa\u4e00\u4efd\u770b\u8d77\u6765\u5408\u7406\u7684\u5cf0\u5217\u8868\u3002<\/p>\n

\u4eba\u5de5\u6587\u4ef6\u4e2d\u5b89\u6392\u4e86\u4e09\u4e2a\u5bcc\u96c6\u533a<\/h2>\n

\u6211\u4eec\u53ea\u4f7f\u7528\u4e00\u6761\u4eba\u5de5\u67d3\u8272\u4f53\uff0c\u5e76\u5c06\u6709\u6548\u57fa\u56e0\u7ec4\u5927\u5c0f\u8bbe\u4e3a\u5341\u4e07\u3002\u6240\u6709\u7247\u6bb5\u957f\u5ea6\u5747\u4e3a\u4e00\u767e\u4e94\u5341\u78b1\u57fa\u3002\u8fd9\u4e2a\u6570\u503c\u662f\u5b9e\u9a8c\u6761\u4ef6\uff0c\u4e0d\u5bf9\u5e94\u4efb\u4f55\u771f\u5b9e\u7269\u79cd\u3002\u771f\u5b9e\u5206\u6790\u9700\u8981\u91c7\u7528\u9002\u5408\u53c2\u8003\u7248\u672c\u548c\u5b9e\u9a8c\u95ee\u9898\u7684\u6709\u6548\u57fa\u56e0\u7ec4\u5927\u5c0f\uff0c\u4e0d\u80fd\u4e3a\u4e86\u5f97\u5230\u66f4\u591a\u5cf0\u7167\u642c\u8fd9\u91cc\u7684\u5c0f\u5206\u6bcd\u3002<\/p>\n

\u7532\u6761\u7801\u5728\u4e00\u4e07\u9644\u8fd1\u6709\u4e8c\u5341\u4e94\u4e2a\u4e0d\u540c\u533a\u95f4\uff0c\u6bcf\u884c\u6b21\u6570\u4e3a\u56db\u3002\u4e59\u6761\u7801\u5728\u4e09\u4e07\u9644\u8fd1\u4e5f\u6709\u4e8c\u5341\u4e94\u4e2a\u4e0d\u540c\u533a\u95f4\uff0c\u6bcf\u884c\u6b21\u6570\u4e3a\u56db\u3002\u53e6\u5728\u4e00\u4e07\u4e94\u5343\u9644\u8fd1\u4e3a\u7532\u6761\u7801\u653e\u7f6e\u4e94\u4e2a\u533a\u95f4\uff0c\u6bcf\u884c\u6b21\u6570\u4e3a\u4e8c\u5341\u3002\u6b64\u5916\uff0c\u6bcf\u4e2a\u6761\u7801\u5404\u6709\u4e00\u767e\u884c\u5206\u6563\u80cc\u666f\uff0c\u6b21\u6570\u5747\u4e3a\u4e00\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n\n
\u8f93\u5165\u90e8\u5206<\/th>\n\u6587\u4ef6\u884c\u6570<\/th>\n\u6bcf\u884c\u6b21\u6570<\/th>\n\u6b21\u6570\u8d21\u732e\u5408\u8ba1<\/th>\n<\/tr>\n<\/thead>\n
\u7532\u6761\u7801\u7b2c\u4e00\u533a\u57df<\/td>\n25<\/td>\n4<\/td>\n100<\/td>\n<\/tr>\n
\u7532\u6761\u7801\u7b2c\u4e8c\u533a\u57df<\/td>\n5<\/td>\n20<\/td>\n100<\/td>\n<\/tr>\n
\u4e59\u6761\u7801\u533a\u57df<\/td>\n25<\/td>\n4<\/td>\n100<\/td>\n<\/tr>\n
\u4e24\u4e2a\u6761\u7801\u7684\u80cc\u666f<\/td>\n200<\/td>\n1<\/td>\n200<\/td>\n<\/tr>\n
\u5408\u8ba1<\/td>\n255<\/td>\n\u4e0d\u540c<\/td>\n500<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u8fd9\u6837\uff0c\u4e8c\u767e\u4e94\u5341\u4e94\u884c\u5bf9\u5e94\u4e94\u767e\u7684\u6b21\u6570\u8d21\u732e\uff0c\u7532\u6761\u7801\u5355\u72ec\u5360\u4e00\u767e\u4e09\u5341\u884c\u3001\u4e09\u767e\u7684\u6b21\u6570\u8d21\u732e\u3002\u6240\u6709\u6570\u5b57\u90fd\u53ef\u4ee5\u4ece\u751f\u6210\u811a\u672c\u76f4\u63a5\u6838\u5bf9\u3002\u4e24\u4e2a\u7532\u533a\u57df\u867d\u7136\u603b\u6b21\u6570\u76f8\u540c\uff0c\u4f46\u4e00\u4e2a\u7531\u4e8c\u5341\u4e94\u884c\u8d21\u732e\uff0c\u53e6\u4e00\u4e2a\u53ea\u7531\u4e94\u884c\u8d21\u732e\uff0c\u9002\u5408\u89c2\u5bdf\u9650\u5236\u6b21\u6570\u540e\u7684\u5dee\u522b\u3002<\/p>\n

\u6b21\u6570\u5217\u5728\u672c\u5b9e\u9a8c\u4e2d\u53ea\u662f\u4eba\u4e3a\u8bbe\u7f6e\u7684\u8f93\u5165\u6743\u91cd\uff0c\u4e0d\u80fd\u81ea\u52a8\u89e3\u91ca\u4e3a\u72ec\u7acb\u5206\u5b50\u6570\u3002\u771f\u5b9e\u7247\u6bb5\u6587\u4ef6\u8fd8\u9700\u8981\u7ed3\u5408\u5176\u751f\u6210\u6d41\u7a0b\u7406\u89e3\u8fd9\u4e00\u5217\uff0c\u5305\u62ec\u662f\u5426\u805a\u5408\u4e86\u91cd\u590d\u89c2\u6d4b\u3002\u628a\u6b21\u6570\u76f8\u52a0\u5f97\u5230\u7684\u91cf\uff0c\u548c\u53bb\u91cd\u540e\u7684\u72ec\u7acb\u8bc1\u636e\u6570\u91cf\uff0c\u4e0d\u80fd\u4ec5\u56e0\u4e3a\u90fd\u88ab\u79f0\u4e3a\u7247\u6bb5\u5c31\u6df7\u4e3a\u4e00\u8c08\u3002<\/p>\n

\u56db\u7ec4\u8c03\u7528\u4fdd\u6301\u4e86\u54ea\u4e9b\u6761\u4ef6<\/h2>\n

\u56db\u7ec4\u4f7f\u7528\u540c\u4e00\u6587\u4ef6\u3001\u76f8\u540c\u6709\u6548\u57fa\u56e0\u7ec4\u5927\u5c0f\u548c\u96f6\u70b9\u96f6\u4e94\u7684\u6821\u6b63\u663e\u8457\u6027\u95e8\u69db\uff0c\u5747\u4fdd\u5b58\u4fe1\u53f7\u8f68\u9053\u548c\u7a84\u5cf0\u7ed3\u679c\u3002\u7b2c\u4e00\u7ec4\u6309 FRAG \u8bfb\u53d6\u5168\u90e8\u6761\u7801\uff0c\u7b2c\u4e8c\u7ec4\u53ea\u9009\u7532\u6761\u7801\uff0c\u7b2c\u4e09\u7ec4\u628a\u6bcf\u884c\u6b21\u6570\u4e0a\u9650\u8bbe\u4e3a\u4e00\uff0c\u7b2c\u56db\u7ec4\u6309\u7b80\u5316 BEDPE \u8bfb\u53d6\u5e76\u4fdd\u7559\u6240\u6709\u8bb0\u5f55\u3002<\/p>\n

\u8fd9\u91cc\u6ca1\u6709\u8f93\u5165\u5bf9\u7167\u6837\u672c\uff0c\u6ca1\u6709\u8fd0\u884c\u5bbd\u5cf0\u5206\u6790\u6216 HMMATAC\uff0c\u4e5f\u6ca1\u6709\u8fdb\u884c\u63d2\u5165\u4f4d\u70b9\u79fb\u52a8\u3002\u7a0b\u5e8f\u5728\u7247\u6bb5\u6a21\u5f0f\u4e0b\u4f7f\u7528\u5b9e\u9645\u533a\u95f4\uff0c\u65e5\u5fd7\u4e2d\u7684\u7247\u6bb5\u957f\u5ea6\u4e3a\u4e00\u767e\u4e94\u5341\uff0c\u5e76\u8df3\u8fc7\u4e86\u5e38\u89c4\u5cf0\u6a21\u578b\u6784\u5efa\u3002\u4e0d\u80fd\u628a\u672c\u6b21\u8fd0\u884c\u63cf\u8ff0\u6210\u5df2\u7ecf\u9a8c\u8bc1\u4e86\u4ece\u771f\u5b9e\u53cc\u7aef\u6bd4\u5bf9\u5f00\u59cb\u7684\u5168\u90e8\u5904\u7406\u3002<\/p>\n

macs3 callpeak -t fragments.tsv -f FRAG \\\r\n  -g 100000 -q 0.05 -n frag_all \\\r\n  --outdir frag_all --threads 2 -B\r\n\r\nmacs3 callpeak -t fragments.tsv -f FRAG \\\r\n  -g 100000 -q 0.05 -n frag_cap1 \\\r\n  --outdir frag_cap1 --threads 2 -B --max-count 1\r\n<\/code><\/pre>\n
\u672c\u6b21\u4f7f\u7528\u56fa\u5b9a\u955c\u50cf\uff0c\u5e76\u8bb0\u5f55\u8f6f\u4ef6\u7248\u672c\u3001\u5b8c\u6574\u5e2e\u52a9\u4fe1\u606f\u4e0e\u6bcf\u7ec4\u547d\u4ee4\u3002\u4e24\u7ebf\u7a0b\u662f\u672c\u5b9e\u9a8c\u7684\u8fd0\u884c\u9884\u7b97\uff0c\u4e0d\u80fd\u636e\u6b64\u63a8\u7b97\u771f\u5b9e\u9879\u76ee\u541e\u5410\u3002\u65e5\u5fd7\u8fd8\u663e\u793a\uff0c\u8fd9\u79cd\u5355\u67d3\u8272\u4f53\u5c0f\u8f93\u5165\u7684\u5cf0\u626b\u63cf\u5b9e\u9645\u91c7\u7528\u4e32\u884c\u8def\u5f84\u3002\u56e0\u6b64\uff0c\u4e0d\u80fd\u4ec5\u56e0\u4e3a\u547d\u4ee4\u91cc\u5199\u4e86\u7ebf\u7a0b\u53c2\u6570\uff0c\u5c31\u58f0\u79f0\u5df2\u7ecf\u6d4b\u5f97\u591a\u7ebf\u7a0b\u52a0\u901f\u6548\u679c\u3002<\/p>\n
\u5cf0\u6570\u91cf\u600e\u6837\u968f\u8ba1\u6570\u53e3\u5f84\u6539\u53d8<\/h2>\n
\u6309 FRAG \u4f7f\u7528\u5168\u90e8\u6b21\u6570\u65f6\uff0c\u4e09\u4e2a\u8bbe\u8ba1\u533a\u57df\u90fd\u8fdb\u5165\u5cf0\u8868\uff0c\u5cf0\u9876\u5904\u7684\u5806\u53e0\u9ad8\u5ea6\u5747\u4e3a\u4e00\u767e\u3002\u628a\u6bcf\u884c\u6b21\u6570\u9650\u5236\u4e3a\u4e00\u540e\uff0c\u65e5\u5fd7\u4e2d\u7684\u603b\u91cf\u53d8\u4e3a\u4e8c\u767e\u4e94\u5341\u4e94\uff0c\u53ea\u4fdd\u7559\u4e00\u4e07\u548c\u4e09\u4e07\u9644\u8fd1\u7684\u4e24\u4e2a\u533a\u57df\uff0c\u4e24\u5904\u5cf0\u9876\u5806\u53e0\u9ad8\u5ea6\u90fd\u53d8\u4e3a\u4e8c\u5341\u4e94\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
\u8fd0\u884c\u6761\u4ef6<\/th>\n\u4f7f\u7528\u7684\u6b21\u6570\u8d21\u732e<\/th>\n\u5cf0\u6570\u91cf<\/th>\n\u4e3b\u8981\u53d8\u5316<\/th>\n<\/tr>\n<\/thead>\n
\u5168\u90e8 FRAG<\/td>\n500<\/td>\n3<\/td>\n\u4e09\u4e2a\u533a\u57df\u4fdd\u7559<\/td>\n<\/tr>\n
\u4ec5\u7532\u6761\u7801<\/td>\n300<\/td>\n2<\/td>\n\u4e59\u6761\u7801\u533a\u57df\u6d88\u5931<\/td>\n<\/tr>\n
\u6bcf\u884c\u6b21\u6570\u4e0a\u9650\u4e3a\u4e00<\/td>\n255<\/td>\n2<\/td>\n\u4e94\u884c\u7ec4\u6210\u7684\u533a\u57df\u6d88\u5931<\/td>\n<\/tr>\n
\u7b80\u5316 BEDPE\uff0c\u4fdd\u7559\u6240\u6709\u884c<\/td>\n255<\/td>\n2<\/td>\n\u4e0e\u4e0a\u9650\u4e3a\u4e00\u7684\u7ed3\u679c\u4e00\u81f4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u5728\u672c\u6b21\u8f93\u5165\u4e0a\uff0c\u7b80\u5316 BEDPE \u4e0e\u6b21\u6570\u4e0a\u9650\u4e3a\u4e00\u7684\u5cf0\u533a\u95f4\u548c\u6570\u503c\u4e00\u81f4\uff0c\u53ea\u662f\u7ed3\u679c\u540d\u79f0\u4e0d\u540c\u3002\u8fd9\u662f\u56e0\u4e3a\u4e24\u8005\u90fd\u8ba9\u6bcf\u884c\u8d21\u732e\u4e00\u6b21\uff0c\u4e14\u5b9e\u9a8c\u533a\u95f4\u6ca1\u6709\u989d\u5916\u7684\u91cd\u590d\u884c\u3002\u4e0d\u80fd\u63a8\u5e7f\u6210\u8fd9\u4e24\u79cd\u5904\u7406\u5728\u6240\u6709\u771f\u5b9e\u6587\u4ef6\u4e2d\u90fd\u7b49\u4ef7\uff0c\u5c24\u5176\u5f53\u8f93\u5165\u5305\u542b\u5176\u4ed6\u91cd\u590d\u7ed3\u6784\u6216\u683c\u5f0f\u5dee\u5f02\u65f6\u3002<\/p>\n
\u4e94\u884c\u7ec4\u6210\u7684\u533a\u57df\u5728\u9650\u5236\u6b21\u6570\u540e\u6ca1\u6709\u8fdb\u5165\u6700\u7ec8\u5cf0\u8868\u3002\u6211\u4eec\u6ca1\u6709\u5c06\u539f\u56e0\u5355\u72ec\u5f52\u4e3a\u67d0\u4e00\u4e2a\u672a\u7ecf\u9694\u79bb\u9a8c\u8bc1\u7684\u95e8\u69db\uff0c\u56e0\u4e3a\u5cf0\u7ed3\u679c\u540c\u65f6\u53d7\u4fe1\u53f7\u3001\u80cc\u666f\u3001\u663e\u8457\u533a\u95f4\u957f\u5ea6\u548c\u5408\u5e76\u89c4\u5219\u5f71\u54cd\u3002\u53ef\u4ee5\u786e\u5b9a\u7684\u662f\uff0c\u4fee\u6539\u6b21\u6570\u5904\u7406\u6539\u53d8\u4e86\u8f93\u5165\u8d21\u732e\uff0c\u7ee7\u800c\u6539\u53d8\u4e86\u5b8c\u6574\u8c03\u7528\u8f93\u51fa\u3002\u82e5\u60f3\u5224\u65ad\u5177\u4f53\u7531\u54ea\u6761\u89c4\u5219\u6dd8\u6c70\uff0c\u8fd8\u9700\u8fdb\u4e00\u6b65\u68c0\u67e5\u76f8\u5e94\u4e2d\u95f4\u8f68\u9053\u548c\u6761\u4ef6\u3002<\/p>\n
\u8fd9\u4e2a\u5bf9\u7167\u6700\u76f4\u63a5\u7684\u7ecf\u9a8c\u662f\uff0c\u4e0d\u8981\u5728\u770b\u5230\u5c11\u4e00\u4e2a\u5cf0\u540e\uff0c\u9a6c\u4e0a\u8ba4\u5b9a\u8f6f\u4ef6\u6f0f\u68c0\u3002\u5148\u6bd4\u8f83\u7a0b\u5e8f\u771f\u6b63\u4f7f\u7528\u4e86\u591a\u5c11\u8bb0\u5f55\u8d21\u732e\uff0c\u4ee5\u53ca\u6539\u53d8\u7684\u662f\u8f93\u5165\u3001\u80cc\u666f\u8fd8\u662f\u8fc7\u6ee4\u89c4\u5219\u3002\u53ea\u7edf\u8ba1\u6587\u4ef6\u884c\u6570\uff0c\u65e0\u6cd5\u89e3\u91ca\u672c\u5b9e\u9a8c\u4e2d\u4e94\u767e\u548c\u4e8c\u767e\u4e94\u5341\u4e94\u4e4b\u95f4\u7684\u5dee\u989d\u3002<\/p>\n
\u6761\u7801\u7b5b\u9009\u8981\u770b\u7b5b\u9009\u4e4b\u540e\u7684\u65e5\u5fd7<\/h2>\n
\u53ea\u9009\u62e9\u7532\u6761\u7801\u65f6\uff0c\u65e5\u5fd7\u5148\u62a5\u544a\u8bfb\u5165\u7684\u603b\u8d21\u732e\u4e3a\u4e94\u767e\uff0c\u968f\u540e\u660e\u786e\u5199\u51fa\u63d0\u53d6\u4e86\u4e09\u767e\u3002\u82e5\u53ea\u641c\u7d22\u7b2c\u4e00\u5904\u603b\u7247\u6bb5\u6570\u91cf\uff0c\u5c31\u4f1a\u8bef\u8ba4\u4e3a\u7b5b\u9009\u6ca1\u6709\u751f\u6548\u3002\u5b8c\u6574\u65e5\u5fd7\u8fd8\u663e\u793a\uff0c\u6b64\u65f6\u7528\u4e8e\u626b\u63cf\u7684\u533a\u95f4\u4f4d\u7f6e\u6570\u91cf\u662f\u4e00\u767e\u4e09\u5341\uff0c\u4e0e\u7532\u6761\u7801\u7684\u6587\u4ef6\u884c\u6570\u5bf9\u5e94\u3002<\/p>\n
\u6700\u7ec8\u8868\u683c\u4e2d\u53ea\u5269\u7532\u6761\u7801\u7684\u4e24\u4e2a\u533a\u57df\uff0c\u4e59\u6761\u7801\u5bf9\u5e94\u7684\u533a\u57df\u6d88\u5931\u3002\u8fd9\u9a8c\u8bc1\u4e86\u672c\u6b21\u6761\u7801\u5217\u8868\u786e\u5b9e\u53c2\u4e0e\u4e86\u7b5b\u9009\u3002\u4f46\u7b5b\u9009\u6210\u529f\u4e0d\u7b49\u4e8e\u7ec6\u80de\u8eab\u4efd\u6b63\u786e\uff0c\u672c\u5b9e\u9a8c\u7684\u6761\u7801\u540d\u79f0\u90fd\u662f\u4eba\u5de5\u6307\u5b9a\uff0c\u6ca1\u6709\u8fdb\u884c\u7ec6\u80de\u8bc6\u522b\u3001\u53cc\u7ec6\u80de\u68c0\u6d4b\u6216\u5206\u7fa4\u3002<\/p>\n
\u5b9e\u9645\u4f7f\u7528\u7ec6\u80de\u5206\u7fa4\u7ed3\u679c\u65f6\uff0c\u5e94\u6838\u5bf9\u6761\u7801\u662f\u5426\u4e0e\u7247\u6bb5\u6587\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u5305\u62ec\u53ef\u80fd\u9644\u52a0\u7684\u6837\u672c\u524d\u7f00\u6216\u540e\u7f00\u3002\u591a\u4e2a\u6837\u672c\u4e4b\u95f4\u82e5\u590d\u7528\u4e86\u76f8\u540c\u6761\u7801\u5b57\u7b26\u4e32\uff0c\u8fd8\u9700\u8981\u786e\u8ba4\u4e0a\u6e38\u5982\u4f55\u533a\u5206\u6837\u672c\u6765\u6e90\u3002\u53ea\u770b\u6761\u7801\u5217\u8868\u957f\u5ea6\uff0c\u4e0d\u80fd\u4fdd\u8bc1\u9009\u5230\u7684\u662f\u9884\u671f\u7684\u90a3\u4e00\u7ec4\u7ec6\u80de\u3002<\/p>\n
macs3 callpeak -t fragments.tsv -f FRAG \\\r\n  --barcodes barcodes_A.txt \\\r\n  -g 100000 -q 0.05 -n frag_CellA \\\r\n  --outdir frag_CellA --threads 2 -B\r\n<\/code><\/pre>\n
\u672c\u6b21\u65e5\u5fd7\u8fd8\u63d0\u793a FRAG \u6a21\u5f0f\u4f1a\u4fdd\u7559\u6b21\u6570\u8d21\u732e\u3002\u82e5\u8981\u6d4b\u8bd5\u8be5\u6a21\u5f0f\u4e2d\u7684\u6b21\u6570\u4e0a\u9650\uff0c\u5e94\u4f7f\u7528\u5bf9\u5e94\u9009\u9879\uff0c\u800c\u4e0d\u662f\u5047\u8bbe\u5176\u4ed6\u683c\u5f0f\u7684\u91cd\u590d\u5904\u7406\u53c2\u6570\u5177\u6709\u540c\u6837\u6548\u679c\u3002\u662f\u5426\u9650\u5236\u6b21\u6570\u5e94\u7531\u6587\u4ef6\u5b9a\u4e49\u548c\u5206\u6790\u76ee\u6807\u51b3\u5b9a\uff0c\u4e0d\u80fd\u56e0\u4e3a\u672c\u4f8b\u4e2d\u5cf0\u66f4\u5c11\uff0c\u5c31\u628a\u67d0\u4e2a\u8bbe\u7f6e\u7edf\u4e00\u79f0\u4f5c\u66f4\u4e25\u683c\u6216\u66f4\u6b63\u786e\u3002<\/p>\n
\u540c\u4e00\u5904\u5cf0\u7684\u9ad8\u5ea6\u76f8\u540c\uff0c\u6821\u6b63\u503c\u4ecd\u53ef\u80fd\u4e0d\u540c<\/h2>\n
\u7532\u6761\u7801\u7b2c\u4e00\u4e2a\u533a\u57df\u5728\u5168\u90e8\u8f93\u5165\u548c\u6761\u7801\u5b50\u96c6\u4e2d\u90fd\u4fdd\u7559\uff0c\u5cf0\u9876\u5806\u53e0\u9ad8\u5ea6\u540c\u4e3a\u4e00\u767e\uff0c\u5176\u8d1f\u5bf9\u6570\u539f\u59cb P \u503c\u4e5f\u76f8\u540c\u3002\u4f46\u8d1f\u5bf9\u6570\u6821\u6b63\u503c\u4ece\u7ea6\u4e00\u767e\u4e8c\u5341\u4e8c\u70b9\u4e09\u4e94\u4e09\u53d8\u4e3a\u4e00\u767e\u4e8c\u5341\u70b9\u4e94\u4e8c\u4e03\u3002\u5019\u9009\u4f4d\u7f6e\u672a\u53d8\uff0c\u4e0d\u4ee3\u8868\u6240\u6709\u7edf\u8ba1\u5217\u5fc5\u987b\u9010\u4f4d\u76f8\u540c\u3002<\/p>\n
\u8fd9\u63d0\u9192\u6211\u4eec\uff0c\u6821\u6b63\u7ed3\u679c\u4e0e\u5f53\u524d\u5206\u6790\u8303\u56f4\u548c\u7edf\u8ba1\u80cc\u666f\u6709\u5173\u3002\u4e0d\u80fd\u628a\u4e0d\u540c\u7ec6\u80de\u96c6\u5408\u5206\u522b\u8c03\u7528\u5cf0\u540e\u5f97\u5230\u7684\u6821\u6b63\u503c\uff0c\u76f4\u63a5\u5f53\u4f5c\u540c\u4e00\u6807\u5c3a\u4e0a\u7684\u7ec6\u80de\u6d3b\u6027\u5f3a\u5f31\u3002\u5373\u4f7f\u67d0\u4e00\u5c40\u90e8\u4fe1\u53f7\u6ca1\u6709\u53d8\u5316\uff0c\u7ed3\u679c\u63d0\u53d6\u7684\u6574\u4f53\u60c5\u5883\u4e5f\u53ef\u80fd\u6539\u53d8\u3002<\/p>\n
\u672c\u5b9e\u9a8c\u6ca1\u6709\u8fdb\u4e00\u6b65\u8fd0\u884c\u5dee\u5f02\u53ef\u53ca\u6027\u5206\u6790\uff0c\u56e0\u6b64\u4e0d\u80fd\u6839\u636e\u4e24\u4e2a\u5cf0\u8868\u7684\u6709\u65e0\u6216\u6821\u6b63\u503c\u5927\u5c0f\uff0c\u5f97\u51fa\u7532\u4e59\u4e24\u7c7b\u7ec6\u80de\u4e4b\u95f4\u5b58\u5728\u7edf\u8ba1\u5b66\u5dee\u5f02\u7684\u7ed3\u8bba\u3002\u9700\u8981\u6bd4\u8f83\u6761\u4ef6\u65f6\uff0c\u5e94\u660e\u786e\u6837\u672c\u6216\u751f\u7269\u5b66\u91cd\u590d\u3001\u5171\u540c\u68c0\u6d4b\u533a\u57df\u53ca\u8ba1\u6570\u6a21\u578b\uff0c\u4e0d\u80fd\u628a\u72ec\u7acb\u8c03\u7528\u7684\u4e24\u4e2a\u95e8\u69db\u7b80\u5355\u76f8\u51cf\u3002<\/p>\n
\u540c\u7406\uff0c\u6781\u5c0f\u7684\u6821\u6b63\u503c\u53ea\u63cf\u8ff0\u5f53\u524d\u6a21\u578b\u4e0b\u7684\u4fe1\u53f7\u4e0e\u80cc\u666f\u5173\u7cfb\uff0c\u4e0d\u4ee3\u8868\u8fd9\u4e00\u533a\u57df\u5df2\u7ecf\u9a8c\u8bc1\u4e86\u67d0\u79cd\u8c03\u63a7\u529f\u80fd\u3002\u4eba\u5de5\u6570\u636e\u4e2d\u7684\u9ad8\u5ea6\u5bcc\u96c6\u662f\u7531\u6211\u4eec\u8bbe\u8ba1\u51fa\u6765\u7684\uff0c\u6070\u597d\u63d0\u9192\u8bfb\u8005\u4e0d\u8981\u628a\u6570\u503c\u6781\u7aef\u4e0e\u751f\u7269\u5b66\u610f\u4e49\u5f3a\u753b\u7b49\u53f7\u3002<\/p>\n
\u8f93\u51fa\u5750\u6807\u9700\u8981\u7528\u540c\u4e00\u5957\u89c4\u5219\u89e3\u91ca<\/h2>\n
\u672c\u6b21\u540c\u4e00\u6761\u5cf0\u5728\u5236\u8868\u7b26\u7ed3\u679c\u4e2d\u7684\u8d77\u70b9\u662f\u4e00\u4e07\u96f6\u56db\uff0c\u5728\u7a84\u5cf0\u6587\u4ef6\u4e2d\u7684\u8d77\u70b9\u662f\u4e00\u4e07\u96f6\u4e09\uff0c\u800c\u7ec8\u70b9\u5747\u4e3a\u4e00\u4e07\u96f6\u4e8c\u767e\u4e00\u5341\u516d\u3002\u524d\u8005\u91c7\u7528\u4ece\u4e00\u5f00\u59cb\u7684\u95ed\u533a\u95f4\u8868\u793a\uff0c\u540e\u8005\u91c7\u7528\u4ece\u96f6\u5f00\u59cb\u7684\u5de6\u95ed\u53f3\u5f00\u8868\u793a\u3002\u4e24\u79cd\u5199\u6cd5\u5bf9\u5e94\u540c\u4e00\u6bb5\u533a\u57df\uff0c\u4e0d\u662f\u7a0b\u5e8f\u628a\u8d77\u70b9\u79fb\u52a8\u4e86\u4e00\u78b1\u57fa\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
\u6838\u5bf9\u5185\u5bb9<\/th>\n\u672c\u6b21\u8f93\u51fa<\/th>\n\u5982\u4f55\u6838\u5bf9<\/th>\n<\/tr>\n<\/thead>\n
\u5236\u8868\u7b26\u5cf0\u533a\u95f4<\/td>\n10004 \u5230 10216<\/td>\n\u957f\u5ea6 213<\/td>\n<\/tr>\n
\u7a84\u5cf0\u533a\u95f4<\/td>\n10003 \u5230 10216<\/td>\n\u7ec8\u70b9\u51cf\u8d77\u70b9\u4e3a 213<\/td>\n<\/tr>\n
\u7a84\u5cf0\u5185\u5cf0\u9876\u504f\u79fb<\/td>\n108<\/td>\n\u52a0\u5230\u96f6\u8d77\u70b9\u5f97\u5230 10111<\/td>\n<\/tr>\n
\u5cf0\u9876\u6587\u4ef6\u533a\u95f4<\/td>\n10111 \u5230 10112<\/td>\n\u8868\u793a\u4e00\u4e2a\u78b1\u57fa\u4f4d\u7f6e<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u5750\u6807\u8f6c\u6362\u6700\u9002\u5408\u7528\u8fd9\u6837\u7684\u5c0f\u4f8b\u5b50\u9a8c\u8bc1\u3002\u628a\u4e24\u4e2a\u8d77\u70b9\u76f4\u63a5\u6bd4\u8f83\u4f1a\u770b\u5230\u5dee\u4e00\uff0c\u4f46\u540c\u65f6\u68c0\u67e5\u533a\u95f4\u957f\u5ea6\u548c\u5cf0\u9876\u4f4d\u7f6e\uff0c\u5c31\u80fd\u786e\u8ba4\u662f\u5426\u53ea\u662f\u5750\u6807\u4f53\u7cfb\u4e0d\u540c\u3002\u82e5\u540e\u7eed\u8981\u4e0e\u57fa\u56e0\u6ce8\u91ca\u6c42\u4ea4\uff0c\u5e94\u5148\u7edf\u4e00\u53c2\u8003\u7248\u672c\u548c\u5750\u6807\u5b9a\u4e49\uff0c\u518d\u89e3\u91ca\u91cd\u53e0\u6570\u91cf\u3002<\/p>\n
\u6587\u4ef6\u540d\u540e\u7f00\u4e5f\u5bb9\u6613\u8bef\u5bfc\u3002\u672c\u6b21\u5e26\u8868\u683c\u540e\u7f00\u7684\u7ed3\u679c\u5b9e\u9645\u662f\u542b\u6ce8\u91ca\u884c\u7684\u5236\u8868\u7b26\u6587\u672c\uff0c\u4e0d\u80fd\u56e0\u4e3a\u540e\u7f00\u770b\u8d77\u6765\u50cf\u7535\u5b50\u8868\u683c\uff0c\u5c31\u6309\u4e8c\u8fdb\u5236\u5de5\u4f5c\u7c3f\u8bfb\u53d6\u3002\u8bfb\u53d6\u65f6\u5e94\u5904\u7406\u5f00\u5934\u8bf4\u660e\u884c\uff0c\u5e76\u4fdd\u7559\u5176\u4e2d\u7684\u7248\u672c\u548c\u53c2\u6570\u4fe1\u606f\uff0c\u4e0d\u5b9c\u53ea\u7559\u4e0b\u6570\u636e\u533a\u540e\u4e22\u5931\u5206\u6790\u6761\u4ef6\u3002<\/p>\n
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python3 run.py run161\r\n<\/code><\/pre>\n
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