{"id":73219,"date":"2026-11-15T15:03:42","date_gmt":"2026-11-15T07:03:42","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73219"},"modified":"2026-11-15T15:03:42","modified_gmt":"2026-11-15T07:03:42","slug":"diffbind-consensus-minoverlap-count-normalized-report-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/diffbind-consensus-minoverlap-count-normalized-report-practice\/","title":{"rendered":"DiffBind \u5b9e\u8dd1\u590d\u76d8\uff0c\u5171\u8bc6\u5cf0\u95e8\u69db\u63d0\u9ad8\u540e\uff0c\u4e3a\u4ec0\u4e48\u5355\u7ec4\u7279\u6709\u7684\u4fe1\u53f7\u6d88\u5931\u4e86"},"content":{"rendered":"
\n

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\u672c\u6587\u5b9e\u9645\u5b89\u88c5\u5e76\u8fd0\u884c DiffBind 3.20.0\uff0c\u6784\u9020\u516d\u4e2a\u6837\u672c\u7684\u5355\u7aef\u6bd4\u5bf9\u6587\u4ef6\u548c\u5cf0\u5217\u8868\uff0c\u5b8c\u6210\u5171\u8bc6\u533a\u57df\u6bd4\u8f83\u3001\u91cd\u65b0\u8ba1\u6570\u3001\u5f52\u4e00\u5316\u3001\u8bbe\u8ba1\u5bf9\u6bd4\u4e0e\u5dee\u5f02\u5206\u6790\u3002\u5e95\u5c42\u4f7f\u7528 DESeq2 1.50.2\uff0c\u8fd0\u884c\u73af\u5883\u4e3a R 4.5.2\u3002\u6240\u6709\u6570\u636e\u5747\u4e3a\u6559\u5b66\u6a21\u62df\uff0c\u6ca1\u6709\u771f\u5b9e\u6837\u672c\uff0c\u6ca1\u6709\u5b9e\u9645\u8c03\u7528\u5cf0\uff0c\u4e5f\u6ca1\u6709\u505a\u5b9e\u9a8c\u5c42\u9762\u7684\u9a8c\u8bc1\u3002<\/p>\n

\"\u95e8\u69db\u63d0\u9ad8\uff0c\u54ea\u4e9b\u4fe1\u53f7\u88ab\u63d0\u524d\u6392\u9664\u4e86\u3002\u516d\u4e2a\u6a21\u62df\u6837\u672c
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\u6a21\u62df\u4e00\u5171\u5b9a\u4e49\u4e09\u767e\u4e2a\u5019\u9009\u533a\u95f4\uff0c\u6bcf\u4e2a\u957f\u4e94\u767e\u78b1\u57fa\uff0c\u76f8\u90bb\u533a\u95f4\u5145\u5206\u5206\u5f00\u3002\u524d\u4e8c\u767e\u516d\u5341\u4e2a\u533a\u95f4\u5728\u6240\u6709\u6837\u672c\u7684\u5cf0\u5217\u8868\u4e2d\u51fa\u73b0\u3002\u968f\u540e\u4e8c\u5341\u4e2a\u53ea\u5199\u5165\u5904\u7406\u7ec4\u7684\u4e09\u4e2a\u5cf0\u5217\u8868\uff0c\u6700\u540e\u4e8c\u5341\u4e2a\u53ea\u5199\u5165\u5bf9\u7167\u7ec4\u7684\u4e09\u4e2a\u5cf0\u5217\u8868\u3002\u56e0\u6b64\uff0c\u6bcf\u4e2a\u5355\u72ec\u7684\u5cf0\u6587\u4ef6\u90fd\u6709\u4e8c\u767e\u516b\u5341\u884c\uff0c\u4f46\u5408\u8d77\u6765\u7684\u5019\u9009\u96c6\u5408\u662f\u4e09\u767e\u4e2a\u3002<\/p>\n

\u6bd4\u5bf9\u6570\u91cf\u7531\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\u751f\u6210\u3002\u540e\u56db\u5341\u4e2a\u533a\u57df\u5206\u522b\u8bbe\u7f6e\u4e86\u5904\u7406\u7ec4\u5347\u9ad8\u6216\u964d\u4f4e\u7684\u5747\u503c\uff0c\u5176\u4f59\u533a\u57df\u4e0d\u8bbe\u7f6e\u7ec4\u95f4\u5747\u503c\u6539\u53d8\u3002\u968f\u540e\u6839\u636e\u6bcf\u4e2a\u533a\u57df\u7684\u8ba1\u6570\u5199\u5165\u76f8\u5e94\u6570\u91cf\u7684\u4eba\u5de5\u6bd4\u5bf9\uff0c\u53e6\u5916\u7ed9\u6bcf\u4e2a\u6837\u672c\u52a0\u5165\u4e00\u5343\u6761\u4f4d\u4e8e\u6240\u6709\u5cf0\u4e4b\u5916\u7684\u80cc\u666f\u8bb0\u5f55\u3002\u6211\u4eec\u56e0\u6b64\u540c\u65f6\u77e5\u9053\u8f93\u5165\u6570\u91cf\u548c\u5e94\u5f53\u88ab\u7edf\u8ba1\u7684\u533a\u57df\u5185\u6570\u91cf\u3002<\/p>\n

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\u4e8c\u3001\u81f3\u5c11\u4e24\u4e2a\u6837\u672c\u6709\u5cf0\uff0c\u4e0d\u8868\u793a\u4e24\u4e2a\u6761\u4ef6\u90fd\u5fc5\u987b\u6709\u5cf0<\/h2>\n

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\n\n\n\n\n\n\n\n\n
\u533a\u57df\u7c7b\u522b<\/th>\n\u652f\u6301\u5cf0\u96c6\u6570<\/th>\n\u81f3\u5c11\u4e24\u4e2a<\/th>\n\u81f3\u5c11\u56db\u4e2a<\/th>\n<\/tr>\n<\/thead>\n
\u4e24\u7ec4\u5171\u540c\u51fa\u73b0<\/td>\n6<\/td>\n\u4fdd\u7559 260<\/td>\n\u4fdd\u7559 260<\/td>\n<\/tr>\n
\u4ec5\u5904\u7406\u7ec4\u51fa\u73b0<\/td>\n3<\/td>\n\u4fdd\u7559 20<\/td>\n\u6392\u9664 20<\/td>\n<\/tr>\n
\u4ec5\u5bf9\u7167\u7ec4\u51fa\u73b0<\/td>\n3<\/td>\n\u4fdd\u7559 20<\/td>\n\u6392\u9664 20<\/td>\n<\/tr>\n
\u5408\u8ba1<\/td>\n\u4e0d\u540c<\/td>\n300<\/td>\n260<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
cons2 <- dba.peakset(d, 1:6, minOverlap=2, bRetrieve=TRUE)\r\ncons4 <- dba.peakset(d, 1:6, minOverlap=4, bRetrieve=TRUE)\r\n<\/code><\/pre>\n
\u8fd9\u91cc\u7684\u95e8\u69db\u65bd\u52a0\u5728\u4f20\u5165\u7684\u5168\u90e8\u5cf0\u96c6\u4e0a\uff0c\u4e0d\u4f1a\u81ea\u52a8\u6539\u5199\u6210\u6bcf\u4e2a\u6761\u4ef6\u5185\u90e8\u7684\u91cd\u590d\u8981\u6c42\u3002\u5728\u516d\u4e2a\u6837\u672c\u7684\u6574\u4f53\u96c6\u5408\u4e2d\u8981\u6c42\u56db\u4efd\u652f\u6301\uff0c\u672c\u4f8b\u4e2d\u7684\u4efb\u4f55\u5355\u7ec4\u7279\u6709\u533a\u57df\u90fd\u4e0d\u53ef\u80fd\u901a\u8fc7\uff0c\u5373\u4f7f\u5b83\u5728\u8be5\u7ec4\u7684\u4e09\u4e2a\u91cd\u590d\u4e2d\u5168\u90e8\u88ab\u8c03\u7528\u4e3a\u5cf0\u3002<\/p>\n
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\u6211\u4eec\u5728\u4e09\u767e\u4e2a\u5171\u8bc6\u533a\u57df\u4e0a\u5bf9\u516d\u4efd\u6bd4\u5bf9\u91cd\u65b0\u8ba1\u6570\uff0c\u5e76\u9010\u4e2a\u68c0\u67e5\u5f97\u5230\u7684\u6574\u6570\u4e0e\u751f\u6210\u65f6\u4fdd\u5b58\u7684\u6570\u91cf\u77e9\u9635\u5b8c\u5168\u4e00\u81f4\u3002\u8fd9\u4e00\u6b65\u6db5\u76d6\u4e00\u5343\u516b\u767e\u4e2a\u533a\u57df\u4e0e\u6837\u672c\u7ec4\u5408\u3002\u6838\u9a8c\u5bf9\u8c61\u662f\u6bcf\u4e00\u683c\u539f\u59cb\u8ba1\u6570\uff0c\u800c\u4e0d\u662f\u53ea\u68c0\u67e5\u603b\u548c\u76f8\u7b49\uff0c\u56e0\u4e3a\u884c\u987a\u5e8f\u9519\u4f4d\u65f6\uff0c\u603b\u548c\u4ecd\u53ef\u80fd\u770b\u8d77\u6765\u6b63\u5e38\u3002<\/p>\n
d <- dba.count(\r\n  d, minOverlap=2, score=DBA_SCORE_READS,\r\n  summits=FALSE, filter=0,\r\n  bUseSummarizeOverlaps=FALSE, fragmentSize=50,\r\n  bRemoveDuplicates=FALSE, bSubControl=FALSE,\r\n  bParallel=FALSE\r\n)\r\nraw <- as.data.frame(dba.peakset(d, bRetrieve=TRUE))\r\n<\/code><\/pre>\n
\u672c\u4f8b\u4f7f\u7528\u5185\u90e8\u8ba1\u6570\u8def\u5f84\uff0c\u8bfb\u6bb5\u548c\u6307\u5b9a\u7247\u6bb5\u957f\u5ea6\u5747\u4e3a\u4e94\u5341\uff0c\u4e14\u90fd\u653e\u5728\u76ee\u6807\u533a\u95f4\u5185\u90e8\uff0c\u4e0d\u8fdb\u884c\u5cf0\u9876\u91cd\u65b0\u5b9a\u4f4d\u3002\u8fd9\u4e9b\u8bbe\u7f6e\u662f\u4e3a\u4e86\u8ba9\u8f93\u5165\u8ba1\u6570\u53ef\u6838\u5bf9\uff0c\u4e0d\u662f\u5bf9\u771f\u5b9e\u53cc\u7aef\u6570\u636e\u3001\u91cd\u590d\u8fc7\u6ee4\u6216\u5bbd\u5cf0\u5206\u6790\u7684\u7edf\u4e00\u5efa\u8bae\u3002\u5c24\u5176\u4e0d\u80fd\u5c06\u672c\u4f8b\u7684\u5355\u7aef\u8bfb\u53d6\u884c\u4e3a\u76f4\u63a5\u5f53\u6210\u771f\u5b9e\u53cc\u7aef\u7247\u6bb5\u8ba1\u6570\u7684\u9a8c\u8bc1\u3002<\/p>\n
\u56db\u3001\u5f52\u4e00\u5316\u7684\u5206\u6bcd\u8981\u80fd\u8ffd\u6eaf\u5230\u8f93\u5165<\/h2>\n
\u8ba1\u6570\u540e\uff0c\u6211\u4eec\u660e\u786e\u9009\u62e9\u6309\u5b8c\u6574\u6587\u5e93\u5927\u5c0f\u8fdb\u884c\u7f29\u653e\uff0c\u8bb0\u5f55\u6bcf\u4e2a\u6837\u672c\u7528\u4e8e\u5f52\u4e00\u5316\u7684\u603b\u8bfb\u6bb5\u6570\u3002\u672c\u6b21\u524d\u4e24\u4e2a\u5bf9\u7167\u6837\u672c\u5206\u522b\u4e3a 32440 \u548c 32504\uff0c\u7b2c\u4e00\u4efd\u5904\u7406\u6837\u672c\u4e3a 36754\u3002\u8fd9\u4e9b\u6570\u5b57\u4e0e\u751f\u6210\u65f6\u533a\u57df\u5185\u8bfb\u6bb5\u603b\u6570\u52a0\u4e0a\u4e00\u5343\u6761\u80cc\u666f\u8bb0\u5f55\u76f8\u7b49\u3002<\/p>\n
\u5904\u7406\u7ec4\u6587\u5e93\u5927\u5c0f\u8f83\u5927\uff0c\u4e0d\u662f\u56e0\u4e3a\u5b83\u7684\u5cf0\u5217\u8868\u884c\u6570\u66f4\u591a\u3002\u4e24\u7ec4\u6bcf\u4e2a\u5cf0\u5217\u8868\u90fd\u662f\u4e8c\u767e\u516b\u5341\u884c\uff0c\u800c\u6bd4\u5bf9\u6570\u91cf\u53d7\u5230\u5404\u533a\u95f4\u6a21\u62df\u8ba1\u6570\u7684\u5f71\u54cd\u3002\u628a\u5cf0\u6570\u91cf\u5f53\u4f5c\u6d4b\u5e8f\u6df1\u5ea6\u5206\u6bcd\uff0c\u4f1a\u6df7\u6dc6\u4e8c\u503c\u7684\u5cf0\u8c03\u7528\u7ed3\u679c\u4e0e\u8fde\u7eed\u7684\u6587\u5e93\u4fe1\u606f\u3002<\/p>\n
d <- dba.contrast(\r\n  d, design="~Condition",\r\n  contrast=c("Condition", "treated", "control")\r\n)\r\nd <- dba.normalize(\r\n  d, method=DBA_DESEQ2,\r\n  normalize=DBA_NORM_LIB,\r\n  library=DBA_LIBSIZE_FULL,\r\n  background=FALSE\r\n)\r\nnorm <- dba.normalize(d, method=DBA_DESEQ2, bRetrieve=TRUE)\r\nd <- dba.analyze(d, method=DBA_DESEQ2, bParallel=FALSE)\r\n<\/code><\/pre>\n
\u5f52\u4e00\u5316\u8bb0\u5f55\u8fd8\u4fdd\u5b58\u4e86\u5b9e\u9645\u7f29\u653e\u56e0\u5b50\uff0c\u516d\u4e2a\u503c\u7ea6\u5728\u96f6\u70b9\u4e5d\u56db\u5230\u4e00\u70b9\u96f6\u516b\u4e4b\u95f4\u3002\u8fd9\u6837\uff0c\u53d1\u73b0\u67d0\u4e2a\u6837\u672c\u5f02\u5e38\u65f6\uff0c\u53ef\u4ee5\u4ece\u5206\u6bcd\u548c\u56e0\u5b50\u5165\u624b\u6392\u67e5\uff0c\u800c\u4e0d\u662f\u4ec5\u51ed\u6700\u7ec8\u663e\u8457\u533a\u57df\u7684\u6570\u91cf\u9009\u62e9\u65b9\u6cd5\u3002\u672c\u6b21\u6ca1\u6709\u8fd0\u884c\u5916\u6e90\u53c2\u7167\u6216\u80cc\u666f\u5206\u7bb1\u5f52\u4e00\u5316\uff0c\u4e5f\u6ca1\u6709\u6bd4\u8f83\u4e0d\u540c\u5f52\u4e00\u5316\u65b9\u6cd5\u7684\u4f18\u52a3\u3002<\/p>\n
\u5982\u679c\u771f\u5b9e\u5b9e\u9a8c\u9884\u671f\u5b58\u5728\u6574\u4f53\u4fe1\u53f7\u5347\u964d\uff0c\u5355\u7eaf\u4f9d\u9760\u603b\u91cf\u7f29\u653e\u662f\u5426\u9002\u5408\uff0c\u5c31\u9700\u8981\u7ed3\u5408\u5b9e\u9a8c\u8bbe\u8ba1\u4e0e\u53c2\u7167\u4fe1\u606f\u8ba8\u8bba\u3002\u672c\u6587\u7684\u5c0f\u6570\u636e\u65e0\u6cd5\u56de\u7b54\u8fd9\u4e00\u95ee\u9898\u3002\u5b83\u80fd\u7ed9\u51fa\u7684\u5b9e\u7528\u7ecf\u9a8c\u662f\uff0c\u9009\u62e9\u65b9\u6cd5\u540e\u5e94\u628a\u65b9\u6cd5\u540d\u3001\u5206\u6bcd\u6765\u6e90\u53ca\u5b9e\u9645\u56e0\u5b50\u4e00\u8d77\u4fdd\u5b58\uff0c\u4f7f\u7ed3\u8bba\u4f9d\u8d56\u7684\u5c3a\u5ea6\u5047\u8bbe\u80fd\u591f\u88ab\u68c0\u67e5\u3002<\/p>\n
\u4e94\u3001\u660e\u786e\u6bd4\u8f83\u65b9\u5411\uff0c\u518d\u89e3\u91ca\u6b63\u8d1f\u53f7<\/h2>\n
\u672c\u6b21\u8bbe\u8ba1\u663e\u5f0f\u5199\u6210\u6761\u4ef6\u56e0\u7d20\uff0c\u5e76\u660e\u786e\u6bd4\u8f83\u201c\u5904\u7406\u76f8\u5bf9\u4e8e\u5bf9\u7167\u201d\u3002\u5bfc\u51fa\u7684\u5bf9\u6bd4\u6458\u8981\u663e\u793a\uff0c\u4e24\u8fb9\u5404\u6709\u4e09\u4e2a\u6837\u672c\u3002\u8fd9\u4e00\u6b65\u503c\u5f97\u5355\u72ec\u68c0\u67e5\uff0c\u56e0\u4e3a\u6837\u672c\u8868\u4e2d\u7684\u7ec4\u540d\u5b58\u5728\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u7ed3\u679c\u8868\u7684\u7b2c\u4e00\u7ec4\u5fc5\u5b9a\u662f\u4f60\u60f3\u8c61\u7684\u5904\u7406\u7ec4\u3002<\/p>\n
\u5728\u5f52\u4e00\u5316\u62a5\u544a\u4e2d\uff0cP261 \u7684\u6548\u5e94\u7ea6\u4e3a\u6b63\u4e00\u70b9\u4e8c\uff0cP281 \u7ea6\u4e3a\u8d1f\u4e8c\u70b9\u4e09\u4e03\u3002\u524d\u8005\u5728\u5904\u7406\u7ec4\u66f4\u9ad8\uff0c\u540e\u8005\u5728\u5bf9\u7167\u7ec4\u66f4\u9ad8\u3002\u6211\u4eec\u53c8\u5efa\u7acb\u53cd\u5411\u5bf9\u6bd4\uff0c\u4f7f\u7528\u76f8\u540c\u8ba1\u6570\u8303\u56f4\u548c\u5f52\u4e00\u5316\uff0c\u91cd\u65b0\u8fd0\u884c\u5206\u6790\u3002\u7ed3\u679c\u4e2d\u6240\u6709\u533a\u57df\u7684\u6548\u5e94\u7b26\u53f7\u53cd\u8f6c\uff0c\u800c\u539f\u59cb\u6982\u7387\u503c\u4fdd\u6301\u4e00\u81f4\uff0c\u811a\u672c\u5bf9\u8fd9\u4e00\u5173\u7cfb\u4f5c\u4e86\u9010\u884c\u6838\u5bf9\u3002<\/p>\n
\u8fd9\u91cc\u7684\u6570\u503c\u662f\u4ee5\u4e8c\u4e3a\u5e95\u7684\u5bf9\u6570\u6548\u5e94\uff0c\u4e0d\u662f\u666e\u901a\u500d\u6570\u3002\u4e00\u70b9\u4e8c\u5bf9\u5e94\u7684\u500d\u6570\u9700\u8981\u901a\u8fc7\u4e8c\u7684\u76f8\u5e94\u6b21\u65b9\u8fd8\u539f\uff0c\u4e0d\u80fd\u5199\u6210\u201c\u63d0\u9ad8\u4e00\u70b9\u4e8c\u500d\u201d\u3002\u5224\u65ad\u65b9\u5411\u65f6\uff0c\u4e5f\u4e0d\u8981\u53ea\u770b\u5217\u540d\u4e2d\u6709\u6b63\u8d1f\u53f7\uff0c\u800c\u5e94\u628a\u5bf9\u6bd4\u5b9a\u4e49\u3001\u7ec4\u522b\u6d53\u5ea6\u548c\u4e00\u4e2a\u5df2\u77e5\u65b9\u5411\u7684\u533a\u57df\u653e\u5728\u4e00\u8d77\u68c0\u67e5\u3002<\/p>\n
\u5bfc\u51fa\u5305\u542b\u6837\u672c\u8ba1\u6570\u7684\u62a5\u544a\u65f6\uff0c\u6837\u672c\u5217\u6309\u6bd4\u8f83\u4e24\u7ec4\u7ec4\u7ec7\u3002\u6211\u4eec\u7684\u539f\u59cb\u77e9\u9635\u5148\u653e\u5bf9\u7167\u3001\u518d\u653e\u5904\u7406\uff0c\u800c\u6b63\u5411\u62a5\u544a\u5148\u5217\u5904\u7406\u3001\u518d\u5217\u5bf9\u7167\u3002\u5982\u679c\u540e\u7eed\u7528\u56fa\u5b9a\u5217\u53f7\u6311\u9009\u6837\u672c\uff0c\u5f88\u5bb9\u6613\u5728\u6ca1\u6709\u62a5\u9519\u7684\u60c5\u51b5\u4e0b\u4ea4\u6362\u5206\u7ec4\u3002\u6309\u660e\u786e\u7684\u6837\u672c\u540d\u79f0\u53d6\u5217\uff0c\u901a\u5e38\u6bd4\u4f9d\u9760\u5217\u4f4d\u7f6e\u66f4\u7a33\u59a5\u3002<\/p>\n
\u516d\u3001\u62a5\u544a\u539f\u59cb\u8ba1\u6570\u65f6\uff0cFold \u5217\u4e5f\u4f1a\u6539\u53d8<\/h2>\n
\u5b9e\u9645\u6838\u5bf9\u4e2d\uff0c\u4e00\u4e2a\u5bb9\u6613\u5ffd\u7565\u7684\u7ec6\u8282\u662f\uff0c\u5173\u95ed\u62a5\u544a\u5f52\u4e00\u5316\u5e76\u4e0d\u53ea\u6539\u53d8\u672b\u5c3e\u7684\u6837\u672c\u8ba1\u6570\u5217\u3002\u672c\u6b21\u540c\u65f6\u5bfc\u51fa\u5f52\u4e00\u5316\u62a5\u544a\u548c\u539f\u59cb\u5c3a\u5ea6\u62a5\u544a\uff0cP261 \u7684 Fold \u4ece\u7ea6\u4e00\u70b9\u4e8c\u53d8\u4e3a\u4e00\u70b9\u4e09\u516b\u4e5d\uff0cP281 \u4ece\u7ea6\u8d1f\u4e8c\u70b9\u4e09\u4e03\u56db\u53d8\u4e3a\u8d1f\u4e8c\u70b9\u4e8c\u4e94\u516b\u3002\u4e24\u4efd\u62a5\u544a\u5bf9\u5e94\u533a\u57df\u7684\u539f\u59cb\u6982\u7387\u503c\u548c\u6821\u6b63\u503c\u5374\u9010\u4e00\u76f8\u540c\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u533a\u57df<\/th>\n\u5f52\u4e00\u5316\u62a5\u544a\u6548\u5e94<\/th>\n\u539f\u59cb\u5c3a\u5ea6\u62a5\u544a\u6548\u5e94<\/th>\n<\/tr>\n<\/thead>\n
P001<\/td>\n0.075<\/td>\n0.379<\/td>\n<\/tr>\n
P261<\/td>\n1.200<\/td>\n1.389<\/td>\n<\/tr>\n
P281<\/td>\n\u22122.374<\/td>\n\u22122.258<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
normalized <- dba.report(\r\n  d, th=1, bCounts=TRUE, bNormalized=TRUE,\r\n  bCalled=TRUE, precision=0\r\n)\r\nraw_scale <- dba.report(\r\n  d, th=1, bCounts=TRUE, bNormalized=FALSE,\r\n  bCalled=TRUE, precision=0\r\n)\r\n<\/code><\/pre>\n
\u539f\u56e0\u5728\u4e8e\u539f\u59cb\u5c3a\u5ea6\u62a5\u544a\u6839\u636e\u539f\u59cb\u8ba1\u6570\u8ba1\u7b97\u7ec4\u522b\u6d53\u5ea6\u53ca\u5176\u5bf9\u6570\u5dee\uff0c\u800c\u5f52\u4e00\u5316\u62a5\u544a\u4f7f\u7528\u5e95\u5c42\u5206\u6790\u63d0\u4f9b\u7684\u6548\u5e94\u3002\u56e0\u6b64\uff0c\u5bfc\u51fa\u539f\u59cb\u5c3a\u5ea6\u4e4b\u540e\uff0c\u4e0d\u80fd\u628a\u5176\u4e2d\u7684\u6548\u5e94\u7ee7\u7eed\u63cf\u8ff0\u6210\u4e0e\u53e6\u4e00\u5f20\u8868\u5b8c\u5168\u76f8\u540c\u7684\u6a21\u578b\u6548\u5e94\u3002\u7edf\u8ba1\u663e\u8457\u6027\u4ecd\u53d6\u81ea\u65e2\u6709\u7684\u5f52\u4e00\u5316\u5206\u6790\uff0c\u5e76\u6ca1\u6709\u56e0\u4e3a\u663e\u793a\u539f\u59cb\u8ba1\u6570\u800c\u91cd\u65b0\u8fdb\u884c\u672a\u5f52\u4e00\u5316\u68c0\u9a8c\u3002<\/p>\n
\u4ee5 P261 \u4e3a\u4f8b\uff0c\u5904\u7406\u7ec4\u539f\u59cb\u8ba1\u6570\u5747\u503c\u7ea6\u4e8c\u767e\u56db\u5341\u4e8c\u70b9\u4e03\uff0c\u5bf9\u7167\u7ec4\u7ea6\u4e5d\u5341\u4e8c\u70b9\u4e03\uff0c\u4e24\u8005\u4e4b\u6bd4\u53d6\u4ee5\u4e8c\u4e3a\u5e95\u7684\u5bf9\u6570\uff0c\u5f97\u5230\u7ea6\u4e00\u70b9\u4e09\u516b\u4e5d\uff0c\u4e0e\u539f\u59cb\u5c3a\u5ea6\u62a5\u544a\u5bf9\u5e94\u3002\u8fd9\u4e2a\u53ef\u4ee5\u624b\u7b97\u7684\u6838\u5bf9\uff0c\u6bd4\u4ec5\u51ed\u8868\u5934\u542b\u6709\u540c\u4e00\u4e2a Fold \u540d\u79f0\u5c31\u8ba4\u4e3a\u542b\u4e49\u76f8\u540c\u66f4\u53ef\u9760\u3002<\/p>\n
\u5728\u4ea4\u4ed8\u4e2d\uff0c\u53ef\u4ee5\u540c\u65f6\u4fdd\u7559\u4e00\u5f20\u7528\u4e8e\u6838\u5bf9\u539f\u59cb\u8ba1\u6570\u7684\u8868\u548c\u4e00\u5f20\u7528\u4e8e\u89e3\u91ca\u6a21\u578b\u6548\u5e94\u7684\u8868\uff0c\u5e76\u5728\u6587\u4ef6\u540d\u4e2d\u6807\u660e\u5c3a\u5ea6\u3002\u6bd4\u8f83\u4e24\u4e2a\u5206\u6790\u7248\u672c\u65f6\uff0c\u4e5f\u5e94\u786e\u8ba4\u62a5\u544a\u53c2\u6570\u4e00\u81f4\u3002\u5426\u5219\uff0c\u4e00\u6b21\u5355\u7eaf\u6539\u53d8\u5bfc\u51fa\u9009\u9879\u7684\u64cd\u4f5c\uff0c\u5c31\u53ef\u80fd\u88ab\u8bef\u5199\u6210\u91cd\u65b0\u5f52\u4e00\u5316\u4e4b\u540e\u6548\u5e94\u53d1\u751f\u53d8\u5316\u3002<\/p>\n
\u4e03\u3001\u901a\u8fc7\u7edf\u8ba1\u95e8\u69db\uff0c\u4ecd\u7136\u4e0d\u662f\u6a21\u62df\u771f\u503c\u7684\u8bc1\u660e<\/h2>\n
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\u8fd9\u53ea\u662f\u4e00\u5957\u56fa\u5b9a\u968f\u673a\u6570\u636e\u548c\u4e00\u4e2a\u660e\u786e\u5206\u6790\u8bbe\u7f6e\u7684\u7ed3\u679c\uff0c\u4e0d\u662f\u591a\u6b21\u6a21\u62df\u7684\u8bef\u5dee\u7387\u8bc4\u4f30\u3002\u4e0d\u80fd\u7528\u4e00\u6b21\u5b9e\u9a8c\u4e2d\u56db\u5341\u56db\u8fd9\u4e2a\u6570\u5b57\u5ba3\u79f0\u5de5\u5177\u51c6\u786e\u7387\uff0c\u4e5f\u4e0d\u80fd\u628a\u663e\u8457\u6027\u9608\u503c\u7406\u89e3\u6210\u6bcf\u6b21\u7ed3\u679c\u540d\u5355\u4e2d\u5fc5\u5b9a\u53ea\u6709\u56fa\u5b9a\u6bd4\u4f8b\u7684\u9519\u8bef\u3002\u82e5\u8981\u8bc4\u4ef7\u7edf\u8ba1\u6027\u8d28\uff0c\u9700\u8981\u53e6\u884c\u8bbe\u8ba1\u91cd\u590d\u6a21\u62df\u3001\u53c2\u6570\u8303\u56f4\u4e0e\u8bc4\u4ef7\u6807\u51c6\u3002<\/p>\n
\u6211\u4eec\u8fd8\u4fdd\u7559\u4e86\u5168\u90e8\u4e09\u767e\u4e2a\u533a\u57df\u7684\u62a5\u544a\uff0c\u800c\u4e0d\u53ea\u4fdd\u5b58\u901a\u8fc7\u9608\u503c\u7684\u56db\u5341\u56db\u884c\u3002\u8fd9\u6837\u53ef\u4ee5\u533a\u5206\u6548\u5e94\u5c0f\u3001\u53d8\u5f02\u5927\u3001\u6821\u6b63\u540e\u8bc1\u636e\u4e0d\u8db3\uff0c\u4ee5\u53ca\u538b\u6839\u6ca1\u6709\u8fdb\u5165\u5019\u9009\u96c6\u5408\u7b49\u60c5\u51b5\u3002\u672c\u6b21\u56db\u652f\u6301\u95e8\u69db\u7684\u4e8c\u767e\u516d\u5341\u4e2a\u533a\u57df\u53ea\u7528\u4e8e\u5171\u8bc6\u96c6\u5408\u5bf9\u7167\uff0c\u6ca1\u6709\u5728\u90a3\u4e2a\u96c6\u5408\u4e0a\u91cd\u65b0\u505a\u4e00\u5957\u5dee\u5f02\u68c0\u9a8c\uff0c\u56e0\u6b64\u4e0d\u80fd\u58f0\u79f0\u5176\u6700\u7ec8\u663e\u8457\u533a\u57df\u6570\u662f\u591a\u5c11\u3002<\/p>\n
\u516b\u3001\u628a\u5206\u6790\u62c6\u6210\u80fd\u591f\u68c0\u67e5\u7684\u51e0\u5c42<\/h2>\n
\u8fd9\u6b21\u5b9e\u8dd1\u5f62\u6210\u4e86\u4e00\u4e2a\u53ef\u8fc1\u79fb\u7684\u68c0\u67e5\u987a\u5e8f\u3002\u5148\u68c0\u67e5\u6837\u672c\u8868\uff0c\u786e\u8ba4\u54ea\u4e2a\u91cd\u590d\u5c5e\u4e8e\u54ea\u4e2a\u6761\u4ef6\u3002\u518d\u68c0\u67e5\u5171\u8bc6\u8303\u56f4\uff0c\u786e\u8ba4\u7814\u7a76\u5173\u5fc3\u7684\u5355\u7ec4\u533a\u57df\u662f\u5426\u4ecd\u5728\u3002\u968f\u540e\u68c0\u67e5\u539f\u59cb\u8ba1\u6570\uff0c\u786e\u8ba4\u6ca1\u6709\u628a\u672a\u8c03\u7528\u5cf0\u9519\u8bef\u586b\u96f6\u3002\u6700\u540e\u68c0\u67e5\u5f52\u4e00\u5316\u3001\u6bd4\u8f83\u65b9\u5411\u53ca\u62a5\u544a\u5c3a\u5ea6\uff0c\u518d\u9605\u8bfb\u663e\u8457\u533a\u57df\u540d\u5355\u3002<\/p>\n
DiffBind \u7684\u4f18\u52bf\u662f\u628a\u5cf0\u96c6\u3001\u6bd4\u5bf9\u8ba1\u6570\u3001\u5b9e\u9a8c\u8bbe\u8ba1\u548c\u5dee\u5f02\u7ed3\u679c\u7ec4\u7ec7\u5728\u540c\u4e00\u6d41\u7a0b\u4e2d\uff0c\u80fd\u591f\u4fdd\u7559\u539f\u59cb\u5cf0\u662f\u5426\u51fa\u73b0\u7684\u4fe1\u606f\u3002\u5b83\u7684\u5c40\u9650\u4e5f\u5728\u4e8e\u524d\u9762\u7684\u9009\u62e9\u4f1a\u6301\u7eed\u4f20\u5230\u540e\u9762\u7684\u7ed3\u679c\u3002\u4e00\u4e2a\u533a\u57df\u88ab\u9009\u5165\uff0c\u8868\u793a\u5b83\u6709\u8d44\u683c\u63a5\u53d7\u5f53\u524d\u5206\u6790\uff0c\u4e0d\u8868\u793a\u5b83\u5fc5\u5b9a\u5dee\u5f02\u663e\u8457\u3002\u4e00\u4e2a\u533a\u57df\u88ab\u6392\u9664\uff0c\u4e5f\u4e0d\u8868\u793a\u5df2\u7ecf\u8bc1\u660e\u4e24\u7ec4\u76f8\u540c\u3002<\/p>\n
\u672c\u4f8b\u6ca1\u6709\u5339\u914d\u7684\u8f93\u5165\u5bf9\u7167\u6bd4\u5bf9\u6587\u4ef6\uff0c\u4e5f\u6ca1\u6709\u9002\u7528\u4e8e\u865a\u6784\u67d3\u8272\u4f53\u7684\u9ed1\u540d\u5355\u6216\u7070\u540d\u5355\uff0c\u56e0\u6b64\u5173\u95ed\u4e86\u76f8\u5e94\u81ea\u52a8\u5904\u7406\u3002\u539f\u59cb\u8ba1\u6570\u4e0e\u751f\u6210\u6570\u91cf\u5b8c\u5168\u4e00\u81f4\uff0c\u662f\u672c\u4f8b\u8ba1\u6570\u6838\u9a8c\u7684\u8bc1\u636e\u3002\u7ed3\u679c\u8bb0\u5f55\u4e2d\u7684\u67d0\u4e2a\u914d\u7f6e\u5b57\u6bb5\u4e0d\u80fd\u72ec\u7acb\u8bc1\u660e\u505a\u8fc7\u67d0\u9879\u5b9e\u9a8c\u5904\u7406\uff0c\u4f8b\u5982\u672c\u6b21\u5f52\u4e00\u5316\u5143\u6570\u636e\u4e2d\u7684\u63a7\u5236\u6263\u9664\u6807\u8bb0\u4e3a\u771f\uff0c\u4e5f\u4e0d\u80fd\u636e\u6b64\u5ba3\u79f0\u5206\u6790\u5b9e\u9645\u4f7f\u7528\u4e86\u4e0d\u5b58\u5728\u7684\u8f93\u5165\u5bf9\u7167\u3002<\/p>\n
\u4e0b\u8f7d\u672c\u7ec4\u4e09\u7bc7\u590d\u73b0\u811a\u672c\u4e0e\u5b9e\u6d4b\u8bb0\u5f55<\/a>\uff0c\u5728\u6709 Docker \u7684 Linux \u73af\u5883\u8fd0\u884c\u4e0b\u9762\u7684\u547d\u4ee4\uff0c\u5373\u53ef\u751f\u6210\u516d\u4efd\u6bd4\u5bf9\u4e0e\u5cf0\u5217\u8868\uff0c\u91cd\u8dd1\u5168\u90e8\u6b65\u9aa4\u3002\u811a\u672c\u56fa\u5b9a\u955c\u50cf\u6458\u8981\uff0c\u5e76\u4fdd\u5b58\u6837\u672c\u8868\u3001\u9884\u671f\u8ba1\u6570\u3001\u5171\u8bc6\u96c6\u5408\u3001\u539f\u59cb\u8ba1\u6570\u3001\u4e24\u79cd\u62a5\u544a\u5c3a\u5ea6\u3001\u53cd\u5411\u5bf9\u6bd4\u548c\u73af\u5883\u4fe1\u606f\u3002\u56fa\u5b9a\u73af\u5883\u7528\u4e8e\u590d\u73b0\u672c\u6b21\u7ed3\u679c\uff0c\u4e0d\u4ee3\u8868\u6700\u65b0\u8f6f\u4ef6\u7ec4\u5408\u3002<\/p>\n
python3 run.py output\r\n<\/code><\/pre>\n
\u8fdb\u4e00\u6b65\u53ef\u67e5\u9605 \u5bf9\u5e94 Bioconductor \u7248\u672c\u7684\u8f6f\u4ef6\u9875<\/a>\u3001DiffBind \u5206\u6790\u6559\u7a0b<\/a> \u548c \u53c2\u6570\u624b\u518c<\/a>\u3002\u540e\u4e24\u4efd\u53d1\u5e03\u7248\u6587\u6863\u53ef\u80fd\u7ee7\u7eed\u66f4\u65b0\uff0c\u672c\u6587\u7684\u5b9e\u6d4b\u6570\u5b57\u4ee5\u968f\u9644\u7248\u672c\u548c\u8bb0\u5f55\u4e3a\u51c6\u3002<\/p>\n
\u5f53\u5019\u9009\u533a\u57df\u3001\u8ba1\u6570\u89c4\u5219\u548c\u6bd4\u8f83\u8bbe\u8ba1\u786e\u5b9a\u540e\uff0c\u82e5\u8981\u5f00\u5c55\u66f4\u591a\u6837\u672c\u7684\u91cd\u590d\u8ba1\u6570\u4e0e\u5dee\u5f02\u5206\u6790\uff0c\u53ef\u643a\u5e26\u6837\u672c\u8868\u3001\u5cf0\u6587\u4ef6\u683c\u5f0f\u548c\u4efb\u52a1\u89c4\u6a21\uff0c\u901a\u8fc7\u4e91\u751f\u4fe1\u751f\u7269\u5206\u6790\u670d\u52a1\u5668\u670d\u52a1<\/a>\u8bc4\u4f30\u8fd0\u884c\u73af\u5883\u4e0e\u6279\u91cf\u5904\u7406\u5b89\u6392\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
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