{"id":73234,"date":"2026-11-15T15:18:37","date_gmt":"2026-11-15T07:18:37","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73234"},"modified":"2026-11-15T15:18:37","modified_gmt":"2026-11-15T07:18:37","slug":"tobias-scorebigwig-negative-signal-zero-footprint-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/tobias-scorebigwig-negative-signal-zero-footprint-practice\/","title":{"rendered":"TOBIAS \u5b9e\u8dd1\u590d\u76d8\uff0c\u8db3\u8ff9\u5206\u6570\u5f88\u9ad8\uff0c\u5c31\u4e00\u5b9a\u770b\u5230\u4e86\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408\u5417"},"content":{"rendered":"
\n

\u5728 ATAC-seq \u8db3\u8ff9\u5206\u6790\u4e2d\uff0c\u6700\u5bb9\u6613\u8bef\u89e3\u7684\u8bcd\u53ef\u80fd\u5c31\u662f\u201c\u8db3\u8ff9\u5206\u6570\u201d\u3002\u4e0d\u5c11\u8bfb\u8005\u76f4\u89c9\u4e0a\u628a\u5b83\u5f53\u4f5c\u7ed3\u5408\u6982\u7387\uff0c\u6216\u8005\u8ba4\u4e3a\u9ad8\u5206\u5904\u5fc5\u7136\u6709\u4e00\u4e2a\u6f02\u4eae\u7684\u4e2d\u592e\u51f9\u9677\u3002\u672c\u6b21\u8fd0\u884c TOBIAS \u7684\u8ba1\u5206\u6a21\u5757\u540e\uff0c\u4e00\u6bb5\u5904\u5904\u4e3a\u5341\u3001\u6ca1\u6709\u4efb\u4f55\u4e2d\u592e\u4e0b\u964d\u7684\u4eba\u5de5\u4fe1\u53f7\uff0c\u4ecd\u7136\u5728\u4e2d\u5fc3\u5f97\u5230\u5341\u5206\u3002\u8fd9\u4e2a\u7ed3\u679c\u4e0d\u662f\u7a0b\u5e8f\u7b97\u9519\u4e86\uff0c\u800c\u662f\u63d0\u9192\u6211\u4eec\uff0c\u5148\u7406\u89e3\u5206\u6570\u7531\u4ec0\u4e48\u7ec4\u6210\uff0c\u518d\u8ba8\u8bba\u5b83\u652f\u6301\u4ec0\u4e48\u7ed3\u8bba\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u8fd0\u884c TOBIAS 0.17.5 \u7684 ScoreBigwig\uff0c\u8f93\u5165\u662f\u81ea\u884c\u7f16\u5199\u7684\u8fde\u7eed\u4fe1\u53f7\u8f68\u9053\u3002\u6211\u4eec\u6ca1\u6709\u8fd0\u884c\u504f\u597d\u6821\u6b63\u3001\u771f\u5b9e\u5e8f\u5217\u626b\u63cf\u6216\u8005\u5dee\u5f02\u7ed3\u5408\u5206\u6790\uff0c\u4e5f\u6ca1\u6709\u4f7f\u7528\u771f\u5b9e\u6d4b\u5e8f\u6837\u672c\u3002\u4e0b\u9762\u7684\u6570\u503c\u7528\u6765\u89e3\u91ca\u8ba1\u5206\u4e0e\u6587\u4ef6\u8bfb\u5199\uff0c\u4e0d\u4ee3\u8868\u67d0\u4e2a\u8f6c\u5f55\u56e0\u5b50\u7684\u5b9e\u9645\u7ed3\u5408\u5f3a\u5ea6\u3002\u9879\u76ee\u6574\u4f53\u529f\u80fd\u53ef\u67e5\u9605\u5b98\u65b9\u4ed3\u5e93<\/a>\u3002<\/p>\n

\"\u6ca1\u6709\u4e2d\u592e\u51f9\u9677\uff0c\u4e5f\u80fd\u5f97\u5230\u9ad8\u5206\u3002\u5b9e\u9645\u8fd0\u884c
\u5b9e\u9645\u8fd0\u884c ScoreBigwig \u00b7 \u56db\u79cd\u4eba\u5de5\u4fe1\u53f7<\/figcaption><\/figure>\n

\u4e00\u3001\u5148\u628a\u4e09\u4e2a\u95ee\u9898\u5206\u5f00<\/h2>\n

\u4e00\u6761\u539f\u59cb\u5207\u5272\u8f68\u9053\u3001\u4e00\u6761\u6821\u6b63\u540e\u8f68\u9053\u548c\u4e00\u6761\u8db3\u8ff9\u8ba1\u5206\u8f68\u9053\uff0c\u867d\u7136\u90fd\u53ef\u80fd\u4fdd\u5b58\u6210\u540c\u6837\u7684\u6587\u4ef6\u683c\u5f0f\uff0c\u4f46\u5e76\u4e0d\u662f\u540c\u4e00\u4e2a\u91cf\u3002\u524d\u8005\u8bb0\u5f55\u5207\u5272\u4fe1\u53f7\uff0c\u6821\u6b63\u6b65\u9aa4\u5c1d\u8bd5\u5904\u7406\u5e8f\u5217\u76f8\u5173\u7684\u5207\u5272\u504f\u597d\uff0c\u8ba1\u5206\u6b65\u9aa4\u518d\u6839\u636e\u90bb\u57df\u5f62\u6001\u4ea7\u751f\u65b0\u7684\u6570\u503c\u3002\u6587\u4ef6\u6269\u5c55\u540d\u4e00\u81f4\uff0c\u5e76\u4e0d\u4ee3\u8868\u5355\u4f4d\u4e0e\u89e3\u91ca\u4e5f\u4e00\u81f4\u3002<\/p>\n

TOBIAS \u4e2d\uff0cATACorrect \u8d1f\u8d23\u76f8\u5e94\u7684\u6821\u6b63\u5de5\u4f5c\uff0cScoreBigwig \u5728\u7ed9\u5b9a\u533a\u57df\u5185\u8ba1\u7b97\u8fde\u7eed\u5206\u6570\uff0cBINDetect \u5219\u8fdb\u4e00\u6b65\u7ed3\u5408\u57fa\u5e8f\u7b49\u4fe1\u606f\u5f00\u5c55\u4f4d\u70b9\u4e0e\u6761\u4ef6\u6bd4\u8f83\u3002\u8fd9\u51e0\u4e2a\u73af\u8282\u8981\u5206\u5f00\u8bb0\u5f55\u8f93\u5165\u6765\u6e90\uff0c\u4e0d\u80fd\u628a\u201c\u6210\u529f\u751f\u6210\u4e00\u6761\u8ba1\u5206\u8f68\u9053\u201d\u5199\u6210\u201c\u5df2\u7ecf\u8bc1\u5b9e\u5dee\u5f02\u7ed3\u5408\u201d\u3002\u6821\u6b63\u73af\u8282\u7684\u8f93\u5165\u4e0e\u8f93\u51fa\u53ef\u5bf9\u7167ATACorrect \u6587\u6863<\/a>\uff0c\u672c\u6587\u53ea\u5b9e\u9645\u6d4b\u8bd5\u4e2d\u95f4\u7684\u8ba1\u5206\u73af\u8282\u3002<\/p>\n

\u672c\u6b21\u76f4\u63a5\u7f16\u5199\u8f68\u9053\uff0c\u662f\u4e3a\u4e86\u8ba9\u6bcf\u4e2a\u4f4d\u7f6e\u7684\u503c\u90fd\u5df2\u77e5\u3002\u5b83\u4e0d\u662f\u901a\u8fc7\u6821\u6b63\u8f6f\u4ef6\u5f97\u5230\u7684\u771f\u5b9e\u6821\u6b63\u7ed3\u679c\uff0c\u66f4\u4e0d\u80fd\u501f\u7528\u6821\u6b63\u5de5\u5177\u7684\u540d\u5b57\u4e3a\u5b83\u589e\u52a0\u5b9e\u9a8c\u53ef\u4fe1\u5ea6\u3002\u8fd9\u6837\u7684\u6784\u9020\u9002\u5408\u56de\u7b54\u201c\u628a\u8d1f\u6570\u622a\u6210\u96f6\u4f1a\u53d1\u751f\u4ec0\u4e48\u201d\uff0c\u5374\u4e0d\u80fd\u56de\u7b54\u771f\u5b9e\u6837\u672c\u4e2d\u7684\u5207\u5272\u504f\u597d\u6709\u6ca1\u6709\u88ab\u5145\u5206\u6d88\u9664\u3002\u628a\u95ee\u9898\u8fb9\u754c\u5199\u6e05\u695a\uff0c\u8bfb\u8005\u624d\u80fd\u77e5\u9053\u54ea\u4e9b\u7ecf\u9a8c\u53ef\u4ee5\u8fc1\u79fb\uff0c\u54ea\u4e9b\u7ed3\u8bba\u4ecd\u9700\u8981\u771f\u6b63\u7684\u5b9e\u9a8c\u6570\u636e\u3002<\/p>\n

\u4e8c\u3001\u56db\u79cd\u4fe1\u53f7\uff0c\u53ea\u6539\u53d8\u4e2d\u5fc3\u548c\u4e24\u4fa7<\/h2>\n

\u6211\u4eec\u6784\u9020\u4e00\u6761\u957f\u5ea6\u4e24\u5343\u4e8c\u767e\u78b1\u57fa\u7684\u865a\u6784\u67d3\u8272\u4f53\uff0c\u5728\u56db\u4e2a\u4e92\u4e0d\u91cd\u53e0\u7684\u533a\u57df\u5199\u5165\u9010\u78b1\u57fa\u6570\u503c\u3002\u6bcf\u6bb5\u4e09\u767e\u78b1\u57fa\uff0c\u4e2d\u5fc3\u7279\u6b8a\u533a\u95f4\u5bbd\u4e8c\u5341\u78b1\u57fa\uff0c\u5176\u4ed6\u4f4d\u7f6e\u7ef4\u6301\u5404\u81ea\u7684\u80cc\u666f\u3002\u4e3a\u4e86\u51cf\u5c11\u8fb9\u7f18\u622a\u65ad\u5bf9\u6f14\u793a\u7684\u5e72\u6270\uff0c\u8bf7\u6c42\u8ba1\u5206\u7684\u533a\u57df\u76f8\u5bf9\u8f93\u5165\u4e24\u7aef\u5404\u5411\u5185\u6536\u7f29\u4e94\u5341\u78b1\u57fa\uff0c\u5e76\u5173\u95ed\u989d\u5916\u533a\u57df\u5ef6\u4f38\u3002<\/p>\n

\u7b2c\u4e00\u6bb5\u5168\u90e8\u4e3a\u5341\uff0c\u79f0\u4e3a\u5e73\u5766\u6bb5\u3002\u7b2c\u4e8c\u6bb5\u4e24\u4fa7\u4e3a\u5341\u3001\u4e2d\u592e\u4e3a\u8d1f\u4e94\uff0c\u79f0\u4e3a\u8d1f\u503c\u51f9\u9677\u6bb5\u3002\u7b2c\u4e09\u6bb5\u4e24\u4fa7\u4e3a\u96f6\u3001\u4e2d\u592e\u4e3a\u8d1f\u4e94\uff0c\u79f0\u4e3a\u5b89\u9759\u80cc\u666f\u51f9\u9677\u6bb5\u3002\u7b2c\u56db\u6bb5\u4e24\u4fa7\u4e3a\u5341\u3001\u4e2d\u592e\u4e3a\u96f6\uff0c\u79f0\u4e3a\u96f6\u503c\u51f9\u9677\u6bb5\u3002\u8f93\u5165\u6587\u4ef6\u4e2d\u8fd9\u4e9b\u96f6\u503c\u662f\u771f\u6b63\u5199\u5165\u7684\u6761\u76ee\uff0c\u4e0d\u662f\u6ca1\u6709\u8986\u76d6\u8be5\u4f4d\u7f6e\uff0c\u6240\u4ee5\u5b83\u4eec\u7684\u751f\u6210\u542b\u4e49\u975e\u5e38\u660e\u786e\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n
\u4eba\u5de5\u533a\u57df<\/th>\n\u4e24\u4fa7\u4fe1\u53f7<\/th>\n\u4e2d\u592e\u4fe1\u53f7<\/th>\n\u4e2d\u5fc3\u8db3\u8ff9\u5206\u6570<\/th>\n<\/tr>\n<\/thead>\n
\u5e73\u5766\u6bb5<\/td>\n10<\/td>\n10<\/td>\n10<\/td>\n<\/tr>\n
\u8d1f\u503c\u51f9\u9677\u6bb5<\/td>\n10<\/td>\n-5<\/td>\n15<\/td>\n<\/tr>\n
\u5b89\u9759\u80cc\u666f\u51f9\u9677\u6bb5<\/td>\n0<\/td>\n-5<\/td>\n5<\/td>\n<\/tr>\n
\u96f6\u503c\u51f9\u9677\u6bb5<\/td>\n10<\/td>\n0<\/td>\n10<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u8fd9\u91cc\u6700\u503c\u5f97\u505c\u4e0b\u6765\u60f3\u7684\u662f\u7b2c\u4e00\u884c\u548c\u7b2c\u56db\u884c\u3002\u89c6\u89c9\u4e0a\uff0c\u4e00\u6bb5\u6ca1\u6709\u51f9\u9677\uff0c\u53e6\u4e00\u6bb5\u4e2d\u592e\u4e0b\u964d\u5230\u4e86\u96f6\uff0c\u4e24\u8005\u7684\u4e2d\u5fc3\u5206\u6570\u5374\u76f8\u540c\u3002\u8fd9\u8bf4\u660e\u201c\u770b\u8d77\u6765\u6709\u51f9\u9677\u201d\u548c\u201c\u5f97\u5230\u66f4\u9ad8\u7684\u5f53\u524d\u8ba1\u5206\u201d\u4e0d\u662f\u540c\u4e49\u8bcd\u3002\u5206\u6570\u7684\u8bbe\u8ba1\u540c\u65f6\u8003\u8651\u90bb\u57df\u53ef\u53ca\u6027\u4e0e\u8d1f\u5411\u4fe1\u53f7\uff0c\u4e0d\u80fd\u7528\u4e00\u6761\u76f4\u89c9\u4e0a\u7684\u8c37\u6df1\u516c\u5f0f\u66ff\u4ee3\u8f6f\u4ef6\u771f\u6b63\u6267\u884c\u7684\u89c4\u5219\u3002<\/p>\n

\u7b2c\u4e09\u884c\u4e5f\u503c\u5f97\u6ce8\u610f\u3002\u5b83\u6709\u8d1f\u5411\u4e2d\u5fc3\uff0c\u4f46\u4e24\u4fa7\u6ca1\u6709\u6b63\u5411\u4fe1\u53f7\uff0c\u7ed3\u679c\u53ea\u6709\u4e94\u5206\uff0c\u4f4e\u4e8e\u5e73\u5766\u9ad8\u4fe1\u53f7\u6bb5\u7684\u5341\u5206\u3002\u82e5\u628a\u5206\u6570\u76f4\u63a5\u53eb\u4f5c\u7ed3\u5408\u6982\u7387\uff0c\u5c31\u65e0\u6cd5\u89e3\u91ca\u4e3a\u4ec0\u4e48\u4e00\u4e2a\u6ca1\u6709\u4e2d\u592e\u8c37\u7684\u533a\u57df\u53cd\u800c\u66f4\u9ad8\u3002\u5206\u6570\u5728\u8fd9\u91cc\u7efc\u5408\u4e86\u591a\u79cd\u7279\u5f81\uff0c\u4e0d\u662f\u53ea\u56de\u7b54\u4e2d\u592e\u662f\u5426\u4e0b\u964d\u3002<\/p>\n

\u4e09\u3001\u56fa\u5b9a\u7a97\u53e3\u4ee5\u540e\uff0c\u624d\u80fd\u8bf4\u6e05\u53c2\u6570\u6539\u4e86\u4ec0\u4e48<\/h2>\n

\u672c\u4f8b\u628a\u8db3\u8ff9\u7a97\u53e3\u548c\u4e24\u4fa7\u7a97\u53e3\u7684\u6700\u5c0f\u3001\u6700\u5927\u957f\u5ea6\u90fd\u8bbe\u4e3a\u4e8c\u5341\uff0c\u4f7f\u6bd4\u8f83\u66f4\u5bb9\u6613\u7406\u89e3\u3002\u5b9e\u9645\u8fd0\u884c\u7684\u57fa\u7840\u547d\u4ee4\u5982\u4e0b\u3002\u5b8c\u6574\u811a\u672c\u8bb0\u5f55\u4e86\u8f6f\u4ef6\u5e2e\u52a9\u4fe1\u606f\u4e0e\u955c\u50cf\u6458\u8981\uff0c\u5e76\u628a\u6bcf\u4e2a\u5bb9\u5668\u9650\u5236\u4e3a\u4e24\u6838\u3001\u56db GiB \u5185\u5b58\u3002<\/p>\n

TOBIAS ScoreBigwig \\\r\n  --signal signal.bw --regions regions.bed \\\r\n  --output footprint.bw --score footprint \\\r\n  --extend 0 \\\r\n  --fp-min 20 --fp-max 20 \\\r\n  --flank-min 20 --flank-max 20 \\\r\n  --cores 2 --split 1\r\n<\/code><\/pre>\n
\u5728\u672c\u6b21\u4f7f\u7528\u7684\u5b9e\u73b0\u4e2d\uff0c\u8db3\u8ff9\u8ba1\u5206\u7ed3\u5408\u4e24\u4fa7\u6b63\u5411\u4fe1\u53f7\u7684\u5747\u503c\u548c\u4e2d\u5fc3\u8d1f\u5411\u90e8\u5206\uff0c\u5e76\u5728\u5019\u9009\u7a97\u53e3\u5bf9\u4f4d\u7f6e\u7684\u8986\u76d6\u5173\u7cfb\u4e2d\u53d6\u76f8\u5e94\u7684\u6700\u5927\u5206\u6570\u3002\u5bf9\u4e8e\u5e73\u5766\u6bb5\uff0c\u6ca1\u6709\u8d1f\u5411\u4e2d\u5fc3\u8d21\u732e\uff0c\u90bb\u57df\u4fe1\u53f7\u4ecd\u7136\u53ef\u4ee5\u4ea7\u751f\u9ad8\u5206\u3002\u56e0\u6b64\uff0c\u65e0\u660e\u663e\u51f9\u9677\u4e5f\u53ef\u80fd\u51fa\u73b0\u8f83\u9ad8\u5f97\u5206\uff0c\u6b63\u662fScoreBigwig \u5b98\u65b9\u8bf4\u660e<\/a>\u5f3a\u8c03\u7684\u89e3\u91ca\u8fb9\u754c\u3002<\/p>\n
\u7406\u89e3\u8fd9\u4e00\u70b9\u4ee5\u540e\uff0c\u68c0\u67e5\u7ed3\u679c\u5c31\u6709\u4e86\u5177\u4f53\u65b9\u6cd5\u3002\u5148\u753b\u8f93\u5165\u8f68\u9053\uff0c\u786e\u8ba4\u8d1f\u5411\u503c\u7a76\u7adf\u4ece\u54ea\u91cc\u6765\uff0c\u518d\u770b\u8f93\u51fa\u5206\u6570\u662f\u5426\u968f\u7a97\u53e3\u957f\u5ea6\u53d8\u5316\u3002\u5982\u679c\u53ea\u4fdd\u5b58\u6700\u7ec8\u5206\u6570\uff0c\u6ca1\u6709\u4fdd\u7559\u4e0a\u6e38\u8f68\u9053\u548c\u53c2\u6570\uff0c\u4e00\u65e6\u7ed3\u679c\u4e0e\u9884\u671f\u4e0d\u4e00\u81f4\uff0c\u5c31\u5f88\u96be\u533a\u5206\u662f\u4fe1\u53f7\u672c\u8eab\u53d8\u4e86\u3001\u7a97\u53e3\u6539\u53d8\u4e86\uff0c\u8fd8\u662f\u6362\u4e86\u4e00\u79cd\u8ba1\u5206\u65b9\u5f0f\u3002<\/p>\n
\u672c\u6b21\u540c\u4e00\u8f93\u5165\u8fd8\u8fd0\u884c\u4e86\u5747\u503c\u6a21\u5f0f\u548c\u4e0d\u53d8\u6362\u6a21\u5f0f\u3002\u5728\u56db\u4e2a\u4e2d\u5fc3\uff0c\u4e8c\u8005\u90fd\u5206\u522b\u8bfb\u51fa\u5341\u3001\u8d1f\u4e94\u3001\u8d1f\u4e94\u3001\u96f6\uff0c\u56e0\u4e3a\u4e2d\u5fc3\u9644\u8fd1\u4e8c\u5341\u78b1\u57fa\u6b63\u597d\u662f\u5e38\u503c\u3002\u8db3\u8ff9\u6a21\u5f0f\u8f93\u51fa\u7684\u5341\u5206\u3001\u5341\u4e94\u5206\u3001\u4e94\u5206\u548c\u5341\u5206\uff0c\u4e0e\u8fd9\u4e9b\u503c\u4e0d\u662f\u76f8\u540c\u91cf\u7eb2\u4e0b\u7684\u7b80\u5355\u66ff\u4ee3\u3002\u6bd4\u8f83\u4e0d\u540c\u6a21\u5f0f\u65f6\uff0c\u5e94\u91cd\u65b0\u6807\u6ce8\u56fe\u4f8b\u548c\u7eb5\u8f74\uff0c\u800c\u4e0d\u662f\u6cbf\u7528\u540c\u4e00\u540d\u79f0\u540e\u76f4\u63a5\u6bd4\u8f83\u9ad8\u4f4e\u3002<\/p>\n
\u56db\u3001\u8d1f\u6570\u4e0d\u662f\u574f\u6570\u636e\uff0c\u5148\u95ee\u5b83\u7684\u6765\u6e90<\/h2>\n
\u5e38\u89c1\u7684\u9884\u5904\u7406\u4e60\u60ef\u662f\u628a\u8d1f\u503c\u88c1\u526a\u6210\u96f6\uff0c\u7406\u7531\u662f\u6d4b\u5e8f\u7247\u6bb5\u6570\u4e0d\u53ef\u80fd\u4e3a\u8d1f\u3002\u4f46\u6821\u6b63\u540e\u8fde\u7eed\u4fe1\u53f7\u4e0e\u539f\u59cb\u7247\u6bb5\u8ba1\u6570\u4e0d\u540c\uff0c\u8d1f\u5411\u503c\u53ef\u80fd\u6b63\u662f\u540e\u7eed\u8db3\u8ff9\u8ba1\u5206\u4f7f\u7528\u7684\u4fe1\u606f\u3002\u5148\u5165\u4e3a\u4e3b\u5730\u6e05\u9664\u8d1f\u6570\uff0c\u53ef\u80fd\u7834\u574f\u6b63\u5728\u7814\u7a76\u7684\u7279\u5f81\u3002<\/p>\n
\u6211\u4eec\u5728\u76f8\u540c\u8f93\u5165\u4e0a\u589e\u52a0\u4e0b\u9650\u4e3a\u96f6\u7684\u53c2\u6570\u91cd\u65b0\u8fd0\u884c\u3002\u8d1f\u503c\u51f9\u9677\u6bb5\u7684\u4e2d\u5fc3\u5206\u6570\u4ece\u5341\u4e94\u4e0b\u964d\u5230\u5341\uff0c\u5b89\u9759\u80cc\u666f\u51f9\u9677\u6bb5\u4ece\u4e94\u4e0b\u964d\u5230\u96f6\uff0c\u5176\u4ed6\u4e24\u4e2a\u4e2d\u5fc3\u4fdd\u6301\u5341\u5206\u3002\u6bcf\u4e00\u884c\u8f93\u5165\u7684\u5750\u6807\u3001\u533a\u57df\u5217\u8868\u548c\u7a97\u53e3\u957f\u5ea6\u90fd\u6ca1\u6709\u6539\u53d8\uff0c\u53d8\u5316\u6765\u81ea\u8fdb\u5165\u8ba1\u7b97\u7684\u6570\u503c\u88ab\u622a\u65ad\u3002\u8fd9\u662f\u53ef\u4ee5\u9010\u9879\u590d\u73b0\u7684\u53c2\u6570\u6548\u5e94\uff0c\u800c\u4e0d\u662f\u4e24\u6b21\u6570\u636e\u4e4b\u95f4\u7684\u751f\u7269\u5b66\u5dee\u5f02\u3002<\/p>\n
TOBIAS ScoreBigwig \\\r\n  --signal signal.bw --regions regions.bed \\\r\n  --output clipped.bw --score footprint \\\r\n  --extend 0 --fp-min 20 --fp-max 20 \\\r\n  --flank-min 20 --flank-max 20 \\\r\n  --cores 2 --split 1 --min-limit 0\r\n<\/code><\/pre>\n
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\u4e94\u3001\u8bfb\u51fa\u6765\u662f\u7f3a\u5931\uff0c\u4e3a\u4ec0\u4e48\u672c\u4f8b\u53ef\u4ee5\u5224\u4e3a\u96f6<\/h2>\n
\u8fd9\u4e00\u8f6e\u8fd8\u6709\u4e00\u4e2a\u6bd4\u8ba1\u5206\u516c\u5f0f\u66f4\u5bb9\u6613\u9020\u6210\u4e0b\u6e38\u9519\u8bef\u7684\u7ec6\u8282\u3002\u67d0\u4e9b\u5df2\u7ecf\u8ba1\u7b97\u4e14\u7ed3\u679c\u4e3a\u96f6\u7684\u4f4d\u7f6e\uff0c\u7528\u8bfb\u53d6\u5e93\u76f4\u63a5\u53d6\u503c\u65f6\u8fd4\u56de\u4e86\u975e\u6570\u503c\u3002\u53ea\u770b\u8fd9\u4e2a\u8fd4\u56de\u7ed3\u679c\uff0c\u5bb9\u6613\u8ba4\u4e3a\u8f6f\u4ef6\u6f0f\u7b97\u4e86\u8be5\u4f4d\u7f6e\uff0c\u6216\u8005\u8ba4\u4e3a\u8fd9\u4e0e\u539f\u59cb\u8f93\u5165\u91cc\u7684\u672a\u77e5\u533a\u57df\u5b8c\u5168\u4e00\u6837\u3002<\/p>\n
\u6211\u4eec\u68c0\u67e5\u4e86\u672c\u6b21\u5b89\u88c5\u7248\u672c\u5b9e\u9645\u4f7f\u7528\u7684\u5199\u51fa\u51fd\u6570\uff0c\u786e\u8ba4\u5b83\u53ea\u628a\u975e\u96f6\u4f4d\u7f6e\u5199\u5165\u8f93\u51fa\u8f68\u9053\u3002\u811a\u672c\u540c\u65f6\u8bb0\u5f55\u4e86\u51fd\u6570\u5185\u5bb9\u7684\u54c8\u5e0c\u4e0e\u68c0\u67e5\u7ed3\u679c\uff0c\u56e0\u6b64\u8fd9\u4e2a\u5224\u65ad\u6765\u81ea\u5f53\u524d\u8fd0\u884c\u7684\u5b9e\u73b0\uff0c\u800c\u4e0d\u53ea\u662f\u63a8\u6d4b\u3002\u539f\u59cb\u8bfb\u53d6\u7ed3\u679c\u5728\u8bb0\u5f55\u4e2d\u4fdd\u7559\u4e3a\u7f3a\u5931\u5f62\u5f0f\uff0c\u53e6\u52a0\u4e00\u4efd\u53ea\u9488\u5bf9\u5df2\u77e5\u8bf7\u6c42\u533a\u57df\u4e2d\u5fc3\u7684\u96f6\u503c\u6062\u590d\u7ed3\u679c\uff0c\u4e8c\u8005\u6ca1\u6709\u6df7\u5199\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u8bfb\u53d6\u60c5\u5f62<\/th>\n\u672c\u4f8b\u4e2d\u5df2\u77e5\u7684\u4e8b\u5b9e<\/th>\n\u53ef\u4ee5\u91c7\u53d6\u7684\u5904\u7406<\/th>\n<\/tr>\n<\/thead>\n
\u5df2\u8bf7\u6c42\u8ba1\u5206\u7684\u4e2d\u5fc3\u8fd4\u56de\u7a7a\u503c<\/td>\n\u5199\u51fa\u51fd\u6570\u7701\u7565\u96f6\u503c\u6761\u76ee<\/td>\n\u53ef\u6309\u672c\u4f8b\u8bed\u4e49\u89e3\u91ca\u4e3a\u96f6<\/td>\n<\/tr>\n
\u8bf7\u6c42\u533a\u57df\u4ee5\u5916\u8fd4\u56de\u7a7a\u503c<\/td>\n\u672a\u9a8c\u8bc1\u8be5\u5904\u5df2\u7ecf\u8ba1\u7b97<\/td>\n\u4e0d\u80fd\u636e\u6b64\u8ba4\u5b9a\u4e3a\u96f6<\/td>\n<\/tr>\n
\u6765\u6e90\u4e0d\u660e\u7684\u5176\u4ed6\u8f68\u9053\u8fd4\u56de\u7a7a\u503c<\/td>\n\u4e0d\u77e5\u9053\u751f\u6210\u4e0e\u5199\u51fa\u89c4\u5219<\/td>\n\u5148\u67e5\u6765\u6e90\u518d\u51b3\u5b9a<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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BINDetect \u6587\u6863<\/a>\u5206\u522b\u63cf\u8ff0\u57fa\u5e8f\u5339\u914d\u3001\u6761\u4ef6\u5206\u6570\u3001\u7ed3\u5408\u5224\u5b9a\u4e0e\u53d8\u5316\u76f8\u5173\u8f93\u51fa\u3002\u8bfb\u8868\u65f6\u5e94\u6309\u7167\u5b57\u6bb5\u542b\u4e49\u89e3\u91ca\uff0c\u4e0d\u80fd\u628a\u57fa\u5e8f\u5339\u914d\u5206\u6570\u3001\u8db3\u8ff9\u5206\u6570\u548c\u6700\u7ec8\u5206\u7c7b\u6df7\u4e3a\u4e00\u5217\u3002\u672c\u6587\u6ca1\u6709\u8fd0\u884c\u8be5\u6a21\u5757\uff0c\u56e0\u6b64\u4e5f\u6ca1\u6709\u7ed9\u51fa\u4efb\u4f55\u771f\u5b9e\u7684\u7ed3\u5408\u6216\u5dee\u5f02\u7ed3\u5408\u5224\u5b9a\u3002<\/p>\n
TOBIAS \u7684\u4f18\u70b9\u662f\u628a\u504f\u597d\u6821\u6b63\u3001\u8fde\u7eed\u8ba1\u5206\u4e0e\u540e\u7eed\u4f4d\u70b9\u89e3\u91ca\u7ec4\u7ec7\u4e3a\u53ef\u8ffd\u8e2a\u7684\u5de5\u5177\u94fe\uff0c\u4fbf\u4e8e\u9010\u9636\u6bb5\u68c0\u67e5\u3002\u5b83\u7684\u9650\u5236\u5728\u4e8e\uff0c\u6bcf\u4e2a\u9636\u6bb5\u4f9d\u8d56\u524d\u9762\u7684\u6570\u636e\u548c\u5047\u8bbe\uff0c\u9ad8\u5206\u5e76\u4e0d\u81ea\u52a8\u7b49\u4e8e\u786e\u5b9a\u7ed3\u5408\uff0c\u57fa\u5e8f\u76f8\u4f3c\u4e5f\u4e0d\u81ea\u52a8\u786e\u5b9a\u5177\u4f53\u5bb6\u65cf\u6210\u5458\u3002\u4e00\u4e2a\u6709\u4ef7\u503c\u7684\u5206\u6790\u62a5\u544a\u5e94\u80fd\u8ba9\u8bfb\u8005\u6cbf\u7740\u7ed3\u8bba\u56de\u5230\u57fa\u5e8f\u3001\u8f68\u9053\u548c\u539f\u59cb\u5904\u7406\u8bb0\u5f55\uff0c\u800c\u4e0d\u53ea\u662f\u5c55\u793a\u6700\u540e\u4e00\u5f20\u70ed\u56fe\u3002<\/p>\n
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\u4e0b\u8f7d\u672c\u7ec4\u590d\u73b0\u811a\u672c\u4e0e\u7ed3\u679c\u8bb0\u5f55<\/a>\uff0c\u5728\u5bf9\u5e94\u76ee\u5f55\u6267\u884c\u4e0b\u9762\u547d\u4ee4\uff0c\u53ef\u91cd\u65b0\u751f\u6210\u56db\u7c7b\u4eba\u5de5\u8f68\u9053\u5e76\u8fd0\u884c\u516d\u79cd\u8ba1\u5206\u8bbe\u7f6e\u3002\u811a\u672c\u8bb0\u5f55 Python 3.11.15\u3001pyBigWig 0.3.25\u3001\u5de5\u5177\u7248\u672c\u3001\u955c\u50cf\u6458\u8981\u3001\u5404\u6b21\u547d\u4ee4\u4e0e\u8f93\u51fa\u4e2d\u5fc3\u503c\u3002\u9700\u8981\u652f\u6301 Docker \u7684 Linux \u73af\u5883\uff0c\u5efa\u8bae\u6bcf\u6b21\u4f7f\u7528\u65b0\u7684\u8f93\u51fa\u76ee\u5f55\u3002<\/p>\n
python3 run.py output\r\n<\/code><\/pre>\n
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