{"id":73275,"date":"2026-11-15T15:50:59","date_gmt":"2026-11-15T07:50:59","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73275"},"modified":"2026-11-15T15:50:59","modified_gmt":"2026-11-15T07:50:59","slug":"methyldackel-overlap-quality-cpg-merge-depth-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/methyldackel-overlap-quality-cpg-merge-depth-practice\/","title":{"rendered":"MethylDackel \u5b9e\u8dd1\u6307\u5357\u2014\u2014\u91cd\u53e0\u8bfb\u6bb5\u3001\u8d28\u91cf\u9608\u503c\u4e0e CpG \u5408\u5e76\uff0c\u600e\u6837\u6539\u53d8\u7532\u57fa\u5316\u8ba1\u6570"},"content":{"rendered":"
\n

\u62ff\u5230\u7532\u57fa\u5316\u63d0\u53d6\u7ed3\u679c\u540e\uff0c\u6700\u5bb9\u6613\u76f4\u63a5\u67e5\u770b\u7684\u662f\u767e\u5206\u6bd4\u3002\u7136\u800c\uff0c\u540c\u6837\u4e00\u4e2a\u767e\u5206\u4e4b\u767e\uff0c\u53ef\u4ee5\u6765\u81ea\u4e00\u4e2a\u901a\u8fc7\u7b5b\u9009\u7684\u7247\u6bb5\uff0c\u4e5f\u53ef\u4ee5\u6765\u81ea\u5927\u91cf\u72ec\u7acb\u7247\u6bb5\u3002\u5b83\u4eec\u5728\u6570\u503c\u4e0a\u76f8\u540c\uff0c\u8bc1\u636e\u5f3a\u5ea6\u5374\u76f8\u5dee\u5f88\u5927\u3002\u82e5\u8fde\u5206\u6bcd\u4e2d\u7684\u201c\u4e00\u4e2a\u201d\u4ee3\u8868\u4ec0\u4e48\u90fd\u6ca1\u6709\u786e\u5b9a\uff0c\u540e\u7eed\u5dee\u5f02\u68c0\u9a8c\u5c31\u53ef\u80fd\u5efa\u7acb\u5728\u9519\u8bef\u7684\u8ba1\u6570\u5355\u4f4d\u4e0a\u3002<\/p>\n

MethylDackel \u4f4d\u4e8e\u6bd4\u5bf9\u4e0e\u7edf\u8ba1\u5206\u6790\u4e4b\u95f4\uff0c\u8d1f\u8d23\u6839\u636e\u53c2\u8003\u5e8f\u5217\u3001\u6bd4\u5bf9\u8bb0\u5f55\u548c\u8fc7\u6ee4\u6761\u4ef6\u6c47\u603b\u7532\u57fa\u5316\u8bc1\u636e\u3002\u672c\u6587\u5b89\u88c5\u5e76\u8fd0\u884c\u4e86 0.6.2 \u7248\u672c\uff0c\u7528\u5341\u516d\u6761\u4eba\u5de5\u6784\u9020\u7684\u6bd4\u5bf9\u8bb0\u5f55\u5206\u522b\u68c0\u67e5\u914d\u5bf9\u91cd\u53e0\u3001\u78b1\u57fa\u8d28\u91cf\u3001\u6bd4\u5bf9\u8d28\u91cf\u3001\u91cd\u590d\u6807\u8bb0\u548c\u94fe\u5408\u5e76\u3002\u8fd9\u91cc\u6ca1\u6709\u8fd0\u884c\u8bfb\u6bb5\u6bd4\u5bf9\u5668\uff0c\u6ca1\u6709\u771f\u5b9e\u6837\u672c\uff0c\u4e5f\u4e0d\u8bc4\u4ef7\u7532\u57fa\u5316\u68c0\u6d4b\u51c6\u786e\u7387\u3002\u5c0f\u6570\u636e\u7684\u4ef7\u503c\uff0c\u662f\u8ba9\u6bcf\u4e00\u6761\u8f93\u51fa\u90fd\u80fd\u8ffd\u6eaf\u5230\u660e\u786e\u7684\u8f93\u5165\u6761\u4ef6\u3002<\/p>\n

\"\u7532\u57fa\u5316\u8ba1\u6570\uff0c\u5148\u5f04\u6e05\u5206\u6bcd\u3002MethylDackel
MethylDackel 0.6.2 \u00b7 \u4eba\u5de5\u6bd4\u5bf9\u8bb0\u5f55\u5b9e\u8dd1<\/figcaption><\/figure>\n

\u4e00\u3001\u5148\u628a\u8f93\u5165\u8fb9\u754c\u68c0\u67e5\u6e05\u695a<\/h2>\n

\u5b98\u65b9\u9879\u76ee\u8bf4\u660e<\/a>\u8981\u6c42\u63d0\u4f9b\u6309\u5750\u6807\u6392\u5e8f\u5e76\u5efa\u7acb\u7d22\u5f15\u7684\u6bd4\u5bf9\u6587\u4ef6\uff0c\u4ee5\u53ca\u5bf9\u5e94\u7684\u53c2\u8003\u5e8f\u5217\u7d22\u5f15\u3002\u5b9e\u9645\u5de5\u4f5c\u4e2d\uff0c\u6587\u4ef6\u80fd\u591f\u6253\u5f00\u53ea\u662f\u7b2c\u4e00\u6b65\uff0c\u8fd8\u8981\u6838\u5bf9\u67d3\u8272\u4f53\u540d\u79f0\u3001\u957f\u5ea6\u3001\u53c2\u8003\u7248\u672c\u548c\u6bd4\u5bf9\u5668\u4f7f\u7528\u7684\u8f6c\u6362\u94fe\u4fe1\u606f\u3002\u53c2\u8003\u7248\u672c\u9519\u8bef\u672a\u5fc5\u603b\u4f1a\u5bfc\u81f4\u7a0b\u5e8f\u9000\u51fa\uff0c\u5374\u53ef\u80fd\u8ba9\u4f4d\u70b9\u4e0a\u4e0b\u6587\u53d1\u751f\u53d8\u5316\u3002<\/p>\n

\u672c\u4f8b\u6784\u9020\u516b\u6761\u5404\u4e09\u767e\u78b1\u57fa\u7684\u53c2\u8003\u5e8f\u5217\uff0c\u6bcf\u6761\u53ea\u6709\u4e00\u4e2a\u7528\u4e8e\u68c0\u67e5\u7684 CpG\u3002\u6b63\u94fe\u80de\u5627\u5576\u7684\u96f6\u8d77\u70b9\u5750\u6807\u662f\u7b2c\u4e00\u767e\u4f4d\uff0c\u76f8\u90bb\u9e1f\u560c\u5464\u4f4d\u4e8e\u7b2c\u4e00\u767e\u96f6\u4e00\u4f4d\u3002\u4e03\u4e2a\u573a\u666f\u5404\u653e\u5165\u4e00\u5bf9\u8bfb\u6bb5\uff0c\u7b2c\u516b\u4e2a\u573a\u666f\u653e\u5165\u4e24\u4e2a\u6765\u81ea\u76f8\u53cd\u8f6c\u6362\u94fe\u7684\u72ec\u7acb\u5355\u7aef\u8bb0\u5f55\u3002\u56e0\u6b64\uff0c\u8f93\u5165\u5171\u6709\u4e03\u5bf9\u52a0\u4e24\u4e2a\u5355\u7aef\u8bb0\u5f55\uff0c\u800c\u4e0d\u662f\u516b\u5bf9\u53cc\u7aef\u6570\u636e\u3002<\/p>\n

\u6bd4\u5bf9\u6587\u4ef6\u4e2d\u7684\u4f4d\u7f6e\u3001\u914d\u5bf9\u6807\u8bb0\u3001\u8d28\u91cf\u503c\u548c\u8f6c\u6362\u94fe\u6807\u7b7e\u5747\u7531\u811a\u672c\u660e\u786e\u5199\u5165\uff0c\u518d\u5b9e\u9645\u4f7f\u7528 Samtools \u6392\u5e8f\u548c\u5efa\u7acb\u7d22\u5f15\u3002\u8fd9\u4e2a\u8bbe\u8ba1\u523b\u610f\u7ed5\u5f00\u6bd4\u5bf9\u4e0d\u786e\u5b9a\u6027\uff0c\u53ea\u89c2\u5bdf\u63d0\u53d6\u7a0b\u5e8f\u5982\u4f55\u5904\u7406\u5df2\u7ed9\u5b9a\u7684\u8bb0\u5f55\u3002\u5c06\u8fd9\u79cd\u7ed3\u679c\u89e3\u91ca\u6210\u67d0\u79cd\u6bd4\u5bf9\u6d41\u7a0b\u7684\u51c6\u786e\u7387\uff0c\u8d85\u51fa\u4e86\u5b9e\u9a8c\u80fd\u652f\u6301\u7684\u8303\u56f4\u3002<\/p>\n

samtools faidx reference.fa\r\nsamtools sort -o input.bam input.sam\r\nsamtools index input.bam\r\nMethylDackel extract -@ 2 -o default reference.fa input.bam\r\n<\/code><\/pre>\n
\u4e8c\u3001\u5148\u770b\u5b9e\u9645\u5217\u6570\uff0c\u518d\u5199\u8bfb\u53d6\u811a\u672c<\/h2>\n
\u672c\u6b21\u9ed8\u8ba4 CpG \u6587\u4ef6\u5b9e\u9645\u6709\u516d\u5217\uff0c\u4f9d\u6b21\u662f\u67d3\u8272\u4f53\u3001\u96f6\u8d77\u70b9\u533a\u95f4\u8d77\u70b9\u3001\u533a\u95f4\u7ec8\u70b9\u3001\u7532\u57fa\u5316\u767e\u5206\u6bd4\u3001\u7532\u57fa\u5316\u8ba1\u6570\u3001\u975e\u7532\u57fa\u5316\u8ba1\u6570\u3002\u5bf9\u4e8e\u4e00\u4e2a\u672a\u5408\u5e76\u7684\u80de\u5627\u5576\uff0c\u533a\u95f4\u662f\u4e00\u78b1\u57fa\u957f\u3002\u4e0b\u9762\u4ee5\u7a7a\u683c\u5c55\u793a\u5b57\u6bb5\u5173\u7cfb\uff0c\u771f\u5b9e\u6587\u4ef6\u4f7f\u7528\u5236\u8868\u7b26\u5206\u9694\u3002<\/p>\n
agree 100 101 100 1 0\r\nstrand_pair 100 101 100 1 0\r\nstrand_pair 101 102 0 0 1\r\n<\/code><\/pre>\n
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\n\n\n\n\n\n\n\n\n
\u4eba\u5de5\u573a\u666f<\/th>\n\u9ed8\u8ba4\u63d0\u53d6\u7ed3\u679c<\/th>\n\u9700\u8981\u907f\u514d\u7684\u8bef\u89e3<\/th>\n<\/tr>\n<\/thead>\n
\u91cd\u53e0\u4e24\u7aef\u5747\u4e3a C\uff0c\u8d28\u91cf\u76f8\u540c<\/td>\n\u7532\u57fa\u5316\u8ba1\u6570\u4e3a 1<\/td>\n\u4e24\u6761\u8bb0\u5f55\u7b49\u4e8e\u4e24\u4e2a\u5206\u5b50<\/td>\n<\/tr>\n
\u91cd\u53e0\u4e24\u7aef C\/T \u51b2\u7a81\uff0c\u8d28\u91cf\u76f8\u540c<\/td>\n\u65e0\u5408\u683c\u8c03\u7528<\/td>\n\u51b2\u7a81\u5c31\u7b49\u4e8e 50% \u7532\u57fa\u5316<\/td>\n<\/tr>\n
C \u7684\u8d28\u91cf 40\uff0cT \u7684\u8d28\u91cf 35<\/td>\n\u9ed8\u8ba4\u4fdd\u7559\uff0c\u95e8\u69db 10 \u65f6\u6d88\u5931<\/td>\n\u53ea\u770b\u539f\u59cb\u8d28\u91cf\u5373\u53ef<\/td>\n<\/tr>\n
\u4e0d\u91cd\u53e0\u914d\u5bf9\uff0c\u53ea\u6709\u4e00\u7aef\u8986\u76d6\u76ee\u6807<\/td>\n\u7532\u57fa\u5316\u8ba1\u6570\u4e3a 1<\/td>\n\u6240\u6709\u914d\u5bf9\u90fd\u5e94\u8d21\u732e\u4e24\u6b21<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8fd9\u91cc\u7684\u7ecf\u9a8c\u4e0d\u662f\u4e00\u5f8b\u628a\u95e8\u69db\u8c03\u4f4e\uff0c\u800c\u662f\u9047\u5230\u8986\u76d6\u6570\u51cf\u5c11\u65f6\uff0c\u5148\u68c0\u67e5\u51cf\u5c11\u53d1\u751f\u5728\u54ea\u4e2a\u73af\u8282\u3002\u539f\u59cb\u8bfb\u6bb5\u8986\u76d6\u3001\u6bd4\u5bf9\u7b5b\u9009\u540e\u8986\u76d6\u3001\u91cd\u53e0\u5904\u7406\u540e\u6709\u6548\u8c03\u7528\u53ef\u4ee5\u4e0d\u540c\u3002\u628a\u8fd9\u51e0\u4e2a\u91cf\u5206\u522b\u8bb0\u5f55\uff0c\u624d\u80fd\u89e3\u91ca\u4e3a\u4ec0\u4e48\u4e00\u4e2a\u770b\u8d77\u6765\u6df1\u5ea6\u5145\u8db3\u7684\u4f4d\u7f6e\uff0c\u5728\u7532\u57fa\u5316\u8868\u91cc\u53ea\u5269\u5f88\u5c11\u8bc1\u636e\u3002<\/p>\n
\u56db\u3001\u6bd4\u5bf9\u8d28\u91cf\u3001\u78b1\u57fa\u8d28\u91cf\u548c\u91cd\u590d\u6807\u8bb0\u5404\u7ba1\u4e00\u4ef6\u4e8b<\/h2>\n
\u6211\u4eec\u53e6\u8bbe\u4e09\u4e2a\u573a\u666f\uff0c\u5206\u522b\u964d\u4f4e\u6bd4\u5bf9\u8d28\u91cf\u3001\u964d\u4f4e\u76ee\u6807\u78b1\u57fa\u8d28\u91cf\uff0c\u6216\u8005\u7ed9\u8bfb\u6bb5\u8bbe\u7f6e\u91cd\u590d\u6807\u8bb0\u3002\u5b83\u4eec\u5728\u9ed8\u8ba4\u7ed3\u679c\u4e2d\u5747\u6ca1\u6709\u7559\u4e0b\u76ee\u6807\u4f4d\u70b9\u3002\u968f\u540e\u6bcf\u6b21\u53ea\u4fee\u6539\u4e00\u79cd\u6761\u4ef6\uff0c\u89c2\u5bdf\u54ea\u4e00\u4e2a\u573a\u666f\u91cd\u65b0\u51fa\u73b0\uff0c\u4ece\u800c\u907f\u514d\u540c\u65f6\u653e\u5bbd\u591a\u4e2a\u53c2\u6570\u540e\u65e0\u6cd5\u5224\u65ad\u539f\u56e0\u3002<\/p>\n
\u6bd4\u5bf9\u8d28\u91cf\u4e3a\u4e94\u7684\u8bb0\u5f55\uff0c\u5728\u8bbe\u7f6e\u6700\u4f4e\u6bd4\u5bf9\u8d28\u91cf\u4e3a\u96f6\u540e\u8fdb\u5165\u7ed3\u679c\u3002\u76ee\u6807\u78b1\u57fa\u8d28\u91cf\u4e3a\u4e09\u7684\u8bb0\u5f55\uff0c\u5728\u6700\u4f4e\u78b1\u57fa\u8d28\u91cf\u6539\u4e3a\u4e00\u540e\u8fdb\u5165\u7ed3\u679c\u3002\u5e26\u91cd\u590d\u6807\u8bb0\u7684\u8bb0\u5f55\uff0c\u5219\u5728\u542f\u7528\u4fdd\u7559\u91cd\u590d\u7684\u9009\u9879\u540e\u8fdb\u5165\u7ed3\u679c\u3002\u4e09\u4e2a\u53d8\u5316\u5bf9\u5e94\u4e09\u4e2a\u4e0d\u540c\u7684\u7b5b\u9009\u5c42\u6b21\uff0c\u4e0d\u80fd\u7528\u4e00\u4e2a\u201c\u6570\u636e\u8d28\u91cf\u8f83\u4f4e\u201d\u6982\u62ec\u6240\u6709\u539f\u56e0\u3002<\/p>\n
MethylDackel extract -q 0 -o mapq0 reference.fa input.bam\r\nMethylDackel extract -p 1 -o base1 reference.fa input.bam\r\nMethylDackel extract --keepDupes -o keepdupes reference.fa input.bam\r\n<\/code><\/pre>\n
\u4fdd\u7559\u91cd\u590d\u6807\u8bb0\u7684\u9009\u9879\uff0c\u5e76\u4e0d\u8d1f\u8d23\u5224\u65ad\u54ea\u4e9b\u8bb0\u5f55\u5e94\u8be5\u88ab\u6807\u8bb0\u4e3a\u91cd\u590d\u3002\u672c\u6587\u4e5f\u6ca1\u6709\u8fd0\u884c\u91cd\u590d\u8bc6\u522b\u7a0b\u5e8f\uff0c\u53ea\u662f\u5728\u4eba\u5de5\u8bb0\u5f55\u4e0a\u8bbe\u7f6e\u4e86\u8fd9\u4e2a\u6807\u5fd7\u3002\u771f\u5b9e\u9879\u76ee\u9700\u8981\u5148\u8bf4\u660e\u91cd\u590d\u6807\u8bb0\u4ece\u54ea\u91cc\u6765\u3001\u662f\u5426\u7b26\u5408\u6587\u5e93\u7c7b\u578b\uff0c\u518d\u51b3\u5b9a\u63d0\u53d6\u65f6\u662f\u5426\u6392\u9664\uff0c\u4e0d\u80fd\u628a\u201c\u4fdd\u7559\u91cd\u590d\u201d\u5199\u6210\u67d0\u79cd\u589e\u52a0\u771f\u5b9e\u8986\u76d6\u7684\u901a\u7528\u6280\u5de7\u3002<\/p>\n
\u672c\u6b21\u8fd8\u4e13\u95e8\u5c1d\u8bd5\u628a\u6700\u4f4e\u78b1\u57fa\u8d28\u91cf\u8bbe\u4e3a\u96f6\u3002\u7a0b\u5e8f\u6ca1\u6709\u4ee5\u975e\u96f6\u72b6\u6001\u9000\u51fa\uff0c\u800c\u662f\u8f93\u51fa\u63d0\u793a\uff0c\u5c06\u8be5\u503c\u91cd\u8bbe\u4e3a\u4e00\uff0c\u5e76\u7ee7\u7eed\u5b8c\u6210\u8fd0\u884c\u3002\u7ed3\u679c\u4e0e\u663e\u5f0f\u8bbe\u4e3a\u4e00\u76f8\u540c\u3002\u8fd9\u8bf4\u660e\u68c0\u67e5\u4efb\u52a1\u6210\u529f\u4e0e\u5426\u4e0d\u80fd\u4ec5\u770b\u9000\u51fa\u7801\uff0c\u8fd8\u5e94\u4fdd\u5b58\u6807\u51c6\u9519\u8bef\u5e76\u6838\u5bf9\u5b9e\u9645\u751f\u6548\u53c2\u6570\u3002\u81ea\u52a8\u5316\u4efb\u52a1\u82e5\u53ea\u5728\u9000\u51fa\u5931\u8d25\u65f6\u901a\u77e5\uff0c\u53ef\u80fd\u6f0f\u6389\u8fd9\u79cd\u53c2\u6570\u88ab\u4fee\u6b63\u7684\u60c5\u51b5\u3002<\/p>\n
\u4e94\u3001CpG \u94fe\u5408\u5e76\u4f1a\u6539\u53d8\u6df1\u5ea6\u7684\u8ba1\u7b97\u5bf9\u8c61<\/h2>\n
\u7b2c\u516b\u4e2a\u573a\u666f\u5305\u542b\u4e24\u4e2a\u72ec\u7acb\u5355\u7aef\u8bb0\u5f55\u3002\u4e00\u4e2a\u652f\u6301\u6b63\u94fe\u80de\u5627\u5576\u7532\u57fa\u5316\uff0c\u53e6\u4e00\u4e2a\u652f\u6301\u76f8\u53cd\u94fe\u5bf9\u5e94\u80de\u5627\u5576\u672a\u7532\u57fa\u5316\u3002\u8fd9\u91cc\u4e0d\u662f\u4e00\u5bf9\u91cd\u53e0\u8bfb\u6bb5\uff0c\u800c\u662f\u4e24\u4e2a\u72ec\u7acb\u8bb0\u5f55\uff0c\u5fc5\u987b\u4e0e\u524d\u9762\u7684\u914d\u5bf9\u51b2\u7a81\u573a\u666f\u533a\u5206\u5f00\u3002<\/p>\n
\u9ed8\u8ba4\u8f93\u51fa\u5c06\u4e24\u6761\u94fe\u5bf9\u5e94\u7684\u80de\u5627\u5576\u5206\u522b\u5217\u51fa\uff0c\u6bcf\u6761\u7684\u6709\u6548\u6df1\u5ea6\u90fd\u662f\u4e00\u3002\u542f\u7528 CpG \u4e0a\u4e0b\u6587\u5408\u5e76\u540e\uff0c\u8f93\u51fa\u533a\u95f4\u8986\u76d6\u76f8\u90bb\u4e24\u78b1\u57fa\uff0c\u7532\u57fa\u5316\u4e0e\u975e\u7532\u57fa\u5316\u8ba1\u6570\u5404\u4e3a\u4e00\uff0c\u767e\u5206\u6bd4\u53d8\u6210\u4e94\u5341\u3002\u53d8\u5316\u6765\u81ea\u8ba1\u6570\u5bf9\u8c61\u7531\u5355\u4e2a\u80de\u5627\u5576\u8f6c\u6210\u5408\u5e76\u540e\u7684 CpG\uff0c\u800c\u4e0d\u662f\u539f\u59cb\u6d4b\u5e8f\u6570\u636e\u589e\u52a0\u4e86\u3002<\/p>\n
MethylDackel extract --mergeContext -o merged reference.fa input.bam\r\nMethylDackel extract -d 2 -o depth2 reference.fa input.bam\r\nMethylDackel extract --mergeContext -d 2 -o merged_depth2 reference.fa input.bam\r\n<\/code><\/pre>\n
\u672a\u5408\u5e76\u65f6\uff0c\u6700\u4f4e\u6df1\u5ea6\u8bbe\u4e3a\u4e8c\uff0c\u6574\u4e2a\u7ed3\u679c\u6ca1\u6709\u4efb\u4f55\u6570\u636e\u884c\u3002\u5408\u5e76\u540e\u518d\u8bbe\u540c\u6837\u95e8\u69db\uff0c\u7b2c\u516b\u4e2a\u573a\u666f\u4fdd\u7559\u4e00\u884c\uff0c\u56e0\u4e3a\u4e24\u6761\u94fe\u5408\u8d77\u6765\u8fbe\u5230\u4e24\u4e2a\u6709\u6548\u8c03\u7528\u3002\u672c\u6b21\u7ed3\u679c\u4e0e\u5b89\u88c5\u7248\u5e2e\u52a9\u4e2d\u6240\u8ff0\u7684\u5408\u5e76\u540e\u5e94\u7528\u6df1\u5ea6\u95e8\u69db\u4e00\u81f4\u3002\u6bd4\u8f83\u4e24\u4e2a\u9879\u76ee\u7684\u201c\u81f3\u5c11\u4e24\u6b21\u8986\u76d6\u201d\u65f6\uff0c\u5982\u679c\u94fe\u5408\u5e76\u8bbe\u7f6e\u4e0d\u540c\uff0c\u5b9e\u9645\u4e0a\u6bd4\u8f83\u7684\u53ef\u80fd\u4e0d\u662f\u540c\u4e00\u4e2a\u8fc7\u6ee4\u6807\u51c6\u3002<\/p>\n
\u8fd8\u8981\u6ce8\u610f\uff0c\u5408\u5e76\u540e\u4e24\u6761\u94fe\u5404\u4e00\u6b21\u652f\u6301\uff0c\u4e0e\u540c\u4e00\u6761\u94fe\u6709\u4e24\u4e2a\u72ec\u7acb\u7247\u6bb5\u5e76\u4e0d\u662f\u5b8c\u5168\u76f8\u540c\u7684\u5b9e\u9a8c\u8986\u76d6\u7ed3\u6784\u3002\u662f\u5426\u9002\u5408\u5408\u5e76\uff0c\u5e94\u7ed3\u5408\u6240\u5173\u6ce8\u7684\u7532\u57fa\u5316\u4e0a\u4e0b\u6587\u548c\u540e\u7eed\u6a21\u578b\u8981\u6c42\u51b3\u5b9a\u3002\u82e5\u5206\u6790\u9700\u8981\u4fdd\u7559\u94fe\u95f4\u4e0d\u5bf9\u79f0\u4fe1\u606f\uff0c\u8fc7\u65e9\u5408\u5e76\u4f1a\u628a\u8fd9\u79cd\u5dee\u5f02\u538b\u7f29\u6210\u4e00\u4e2a\u5e73\u5747\u503c\u3002<\/p>\n
\u516d\u3001\u7f3a\u5931\u884c\u3001\u96f6\u6bd4\u4f8b\u548c\u96f6\u8986\u76d6\u8981\u5206\u522b\u89e3\u91ca<\/h2>\n
\u9ed8\u8ba4\u8f93\u51fa\u4e2d\u6ca1\u6709\u67d0\u4e2a\u4f4d\u70b9\uff0c\u53ef\u80fd\u662f\u6ca1\u6709\u8bfb\u6bb5\u8986\u76d6\uff0c\u4e5f\u53ef\u80fd\u662f\u8bfb\u6bb5\u88ab\u6bd4\u5bf9\u8d28\u91cf\u3001\u78b1\u57fa\u8d28\u91cf\u6216\u6807\u8bb0\u7b5b\u6389\uff0c\u8fd8\u53ef\u80fd\u662f\u914d\u5bf9\u51b2\u7a81\u5bfc\u81f4\u6ca1\u6709\u5408\u683c\u8c03\u7528\u3002\u5355\u51ed\u8f93\u51fa\u8868\u7f3a\u4e00\u884c\uff0c\u65e0\u6cd5\u5224\u5b9a\u8fd9\u4e2a\u4f4d\u70b9\u5b8c\u5168\u672a\u7532\u57fa\u5316\uff0c\u66f4\u4e0d\u80fd\u5728\u8de8\u6837\u672c\u5408\u5e76\u65f6\u76f4\u63a5\u8865\u6210\u767e\u5206\u4e4b\u96f6\u3002<\/p>\n
\u672c\u6b21\u80de\u5627\u5576\u62a5\u544a\u6a21\u5f0f\u8f93\u51fa\u5341\u516d\u884c\uff0c\u5bf9\u5e94\u516b\u4e2a CpG \u7684\u4e24\u6761\u94fe\u3002\u5176\u4e2d\u6709\u4e9b\u884c\u7684\u7532\u57fa\u5316\u8ba1\u6570\u4e0e\u975e\u7532\u57fa\u5316\u8ba1\u6570\u90fd\u662f\u96f6\u3002\u8fd9\u6837\u7684\u96f6\u9664\u4ee5\u96f6\u6ca1\u6709\u53ef\u89e3\u91ca\u7684\u7532\u57fa\u5316\u6bd4\u4f8b\uff0c\u5e94\u5f53\u4f5c\u4e3a\u7f3a\u4e4f\u6709\u6548\u89c2\u6d4b\u5904\u7406\u3002\u4e0e\u4e4b\u4e0d\u540c\uff0c\u4e00\u4e2a\u7532\u57fa\u5316\u8ba1\u6570\u4e3a\u96f6\u3001\u975e\u7532\u57fa\u5316\u8ba1\u6570\u4e3a\u4e00\u7684\u4f4d\u7f6e\uff0c\u624d\u5177\u6709\u4e00\u6b21\u652f\u6301\u672a\u7532\u57fa\u5316\u7684\u8c03\u7528\u3002<\/p>\n
\u6bd4\u4f8b\u6a21\u5f0f\u53c8\u5e26\u6765\u53e6\u4e00\u79cd\u533a\u522b\u3002\u542f\u7528\u6bd4\u4f8b\u8f93\u51fa\u540e\uff0c\u672c\u6b21\u6587\u4ef6\u53d8\u4e3a\u56db\u5217\uff0c\u7b2c\u56db\u5217\u4f7f\u7528\u4e00\u4e0e\u96f6\uff0c\u4e0d\u518d\u4fdd\u7559\u4e24\u79cd\u8ba1\u6570\u3002\u8fd9\u6837\u7684\u6587\u4ef6\u9002\u5408\u67d0\u4e9b\u5c55\u793a\u7528\u9014\uff0c\u4f46\u82e5\u540e\u7eed\u7edf\u8ba1\u9700\u8981\u8ba1\u6570\u53ca\u8986\u76d6\u4fe1\u606f\uff0c\u4ec5\u4fdd\u7559\u6bd4\u4f8b\u4f1a\u635f\u5931\u5fc5\u8981\u8f93\u5165\u3002\u767e\u5206\u6bd4\u3001\u4e00\u4f4d\u5c0f\u6570\u7684\u6bd4\u4f8b\u548c\u6574\u6570\u8ba1\u6570\u4e4b\u95f4\uff0c\u4e0d\u80fd\u9760\u7b80\u5355\u6539\u5217\u540d\u4e92\u76f8\u66ff\u4ee3\u3002<\/p>\n
\u4e03\u3001M-bias \u56fe\u5e94\u8be5\u670d\u52a1\u4e8e\u5177\u4f53\u7684\u4fee\u526a\u51b3\u5b9a<\/h2>\n
\u672c\u6b21\u5b9e\u9645\u8fd0\u884c\u4e86 M-bias \u5b50\u547d\u4ee4\u5e76\u751f\u6210\u76f8\u5e94\u56fe\u6587\u4ef6\uff0c\u4f46\u5341\u516d\u6761\u4eba\u5de5\u8bb0\u5f55\u4e0d\u8db3\u4ee5\u4f30\u8ba1\u771f\u5b9e\u6587\u5e93\u7684\u672b\u7aef\u504f\u501a\uff0c\u6240\u4ee5\u672c\u6587\u4e0d\u4ece\u8fd9\u4e9b\u56fe\u63a8\u5bfc\u4fee\u526a\u957f\u5ea6\u3002\u5b9e\u8dd1\u4e00\u4e2a\u547d\u4ee4\uff0c\u4e0e\u5f97\u5230\u8db3\u591f\u652f\u6301\u67d0\u9879\u5b9e\u9a8c\u5224\u65ad\u7684\u6570\u636e\uff0c\u662f\u4e24\u4ef6\u9700\u8981\u5206\u522b\u8bf4\u660e\u7684\u4e8b\u3002<\/p>\n
\u771f\u5b9e\u6570\u636e\u4e2d\u7684\u672b\u7aef\u5f02\u5e38\uff0c\u5e94\u540c\u65f6\u68c0\u67e5\u8f6c\u6362\u94fe\u3001\u8bfb\u6bb5\u7aef\u522b\u3001\u8986\u76d6\u6570\u91cf\u548c\u504f\u79bb\u662f\u5426\u5728\u591a\u4e2a\u6837\u672c\u4e2d\u4e00\u81f4\u3002\u53ea\u6709\u6781\u5c11\u6570\u8bfb\u6bb5\u8d21\u732e\u7684\u672b\u7aef\u6ce2\u52a8\uff0c\u4e0e\u5927\u91cf\u8986\u76d6\u4e0b\u6301\u7eed\u51fa\u73b0\u7684\u7cfb\u7edf\u504f\u79fb\uff0c\u5904\u7406\u4f9d\u636e\u4e0d\u540c\u3002\u628a\u67d0\u7bc7\u6559\u7a0b\u4e2d\u7684\u56fa\u5b9a\u4fee\u526a\u957f\u5ea6\u76f4\u63a5\u5957\u5230\u6240\u6709\u6587\u5e93\uff0c\u53ef\u80fd\u79fb\u9664\u6b63\u5e38\u4fe1\u53f7\uff0c\u4e5f\u53ef\u80fd\u7559\u4e0b\u771f\u6b63\u53d7\u5f71\u54cd\u7684\u4f4d\u7f6e\u3002<\/p>\n
\u63d0\u53d6\u524d\u540e\u7684\u4f4d\u70b9\u6570\u91cf\u53ef\u4ee5\u5e2e\u52a9\u5224\u65ad\u4fee\u526a\u5f71\u54cd\uff0c\u4f46\u4e0d\u80fd\u53ea\u8ffd\u6c42\u4fdd\u7559\u66f4\u591a\u3002\u66f4\u597d\u7684\u505a\u6cd5\u662f\u9884\u5148\u8bb0\u5f55\u4fee\u526a\u4f9d\u636e\uff0c\u4f7f\u7528\u540c\u4e00\u5957\u660e\u786e\u89c4\u5219\u5904\u7406\u53ef\u6bd4\u6837\u672c\uff0c\u7136\u540e\u68c0\u67e5\u53d8\u5316\u662f\u5426\u96c6\u4e2d\u4e8e\u9884\u671f\u4f4d\u7f6e\u3002\u82e5\u4e0d\u540c\u6279\u6b21\u9700\u8981\u4e0d\u540c\u5904\u7406\uff0c\u4e5f\u5e94\u4fdd\u5b58\u7406\u7531\uff0c\u907f\u514d\u5728\u770b\u5230\u5dee\u5f02\u7ed3\u679c\u540e\u624d\u53cd\u5411\u6311\u9009\u53c2\u6570\u3002<\/p>\n
\u516b\u3001\u8fd9\u4e2a\u5de5\u5177\u9002\u5408\u89e3\u51b3\u4ec0\u4e48\u95ee\u9898<\/h2>\n
MethylDackel \u7684\u5b9e\u7528\u4e4b\u5904\uff0c\u662f\u628a\u91cd\u53e0\u3001\u8d28\u91cf\u548c\u4e0a\u4e0b\u6587\u5408\u5e76\u7b49\u5173\u952e\u89c4\u5219\u96c6\u4e2d\u5230\u660e\u786e\u7684\u63d0\u53d6\u6b65\u9aa4\u4e2d\uff0c\u4fbf\u4e8e\u5efa\u7acb\u53ef\u68c0\u67e5\u7684\u4f4d\u70b9\u8ba1\u6570\u8868\u3002\u5b83\u4e5f\u63d0\u4f9b\u4e86\u591a\u79cd\u8f93\u51fa\u65b9\u5f0f\uff0c\u53ef\u4ee5\u9002\u914d\u4e0d\u540c\u540e\u7eed\u4efb\u52a1\u3002\u4ee3\u4ef7\u662f\u7528\u6237\u9700\u8981\u771f\u6b63\u7406\u89e3\u6bcf\u79cd\u8f93\u51fa\u7684\u5355\u4f4d\uff0c\u4e0d\u80fd\u4ee5\u4e3a\u6240\u6709\u4ee5\u7532\u57fa\u5316\u4e3a\u540d\u7684\u8868\u90fd\u80fd\u76f4\u63a5\u4e92\u6362\u3002<\/p>\n
\u5b83\u4e0d\u4f1a\u66ff\u4ee3\u6587\u5e93\u8bbe\u8ba1\u3001\u53c2\u8003\u6838\u9a8c\u3001\u91cd\u590d\u8bc6\u522b\u6216\u8005\u5dee\u5f02\u7edf\u8ba1\u3002\u4e00\u4e2a\u683c\u5f0f\u5b8c\u6574\u7684\u7ed3\u679c\uff0c\u4e5f\u4e0d\u4f1a\u81ea\u52a8\u8bf4\u660e\u8f6c\u6362\u5145\u5206\u3001\u6837\u672c\u53ef\u6bd4\u6216\u751f\u7269\u5b66\u91cd\u590d\u8db3\u591f\u3002\u9009\u5de5\u5177\u65f6\uff0c\u5148\u786e\u8ba4\u4e0a\u6e38\u6bd4\u5bf9\u683c\u5f0f\u53d7\u652f\u6301\u3001\u94fe\u4fe1\u606f\u6b63\u786e\u3001\u4e0b\u6e38\u9700\u8981\u8ba1\u6570\u8fd8\u662f\u6bd4\u4f8b\uff0c\u518d\u8003\u8651\u5177\u4f53\u53c2\u6570\uff0c\u6bd4\u5148\u5217\u51fa\u4e00\u957f\u4e32\u4f18\u5316\u5f00\u5173\u66f4\u6709\u6548\u3002<\/p>\n
\u4e5d\u3001\u600e\u6837\u590d\u73b0\u5e76\u8fc1\u79fb\u5230\u81ea\u5df1\u7684\u6570\u636e<\/h2>\n
\u4e0b\u8f7d\u672c\u7ec4\u5b8c\u6574\u811a\u672c\u4e0e\u5b9e\u8dd1\u8bb0\u5f55<\/a>\uff0c\u5728\u672c\u7bc7\u76ee\u5f55\u6267\u884c\u4e0b\u9762\u547d\u4ee4\u5373\u53ef\u91cd\u65b0\u6784\u9020\u53c2\u8003\u548c\u6bd4\u5bf9\u8bb0\u5f55\u3002\u811a\u672c\u56fa\u5b9a\u5bb9\u5668\u955c\u50cf\u6458\u8981\uff0c\u4fdd\u5b58\u6bcf\u6761\u5b9e\u9645\u547d\u4ee4\u3001\u6807\u51c6\u8f93\u51fa\u3001\u6807\u51c6\u9519\u8bef\u548c\u63d0\u53d6\u7ed3\u679c\u3002\u5bb9\u5668\u9650\u5236\u4e3a\u4e24\u6838\u3001\u56db GiB \u5185\u5b58\uff0c\u8fd9\u662f\u672c\u4f8b\u7684\u6267\u884c\u8fb9\u754c\uff0c\u4e0d\u6784\u6210\u5168\u57fa\u56e0\u7ec4\u6570\u636e\u7684\u8d44\u6e90\u5efa\u8bae\u3002<\/p>\n
python3 run.py output\r\n<\/code><\/pre>\n
\u8fc1\u79fb\u5230\u771f\u5b9e\u9879\u76ee\u65f6\uff0c\u53ef\u4ee5\u5148\u62bd\u53d6\u5c11\u91cf\u5df2\u77e5\u4f4d\u70b9\uff0c\u4eba\u5de5\u6838\u5bf9\u8bfb\u6bb5\u91cd\u53e0\u548c\u8fc7\u6ee4\u539f\u56e0\uff0c\u518d\u6269\u5c55\u5230\u6574\u4e2a\u6837\u672c\u3002\u91cd\u70b9\u4fdd\u7559\u7248\u672c\u3001\u53c2\u8003\u6765\u6e90\u3001\u91cd\u590d\u5904\u7406\u3001\u8d28\u91cf\u9608\u503c\u3001\u94fe\u5408\u5e76\u72b6\u6001\u548c\u8f93\u51fa\u5355\u4f4d\u3002\u8fd9\u4e9b\u8bb0\u5f55\u80fd\u8ba9\u540e\u7eed\u5dee\u5f02\u5206\u6790\u660e\u786e\u77e5\u9053\u8f93\u5165\u4e2d\u7684\u4e00\u4e2a\u8ba1\u6570\u5230\u5e95\u610f\u5473\u7740\u4ec0\u4e48\uff0c\u4e5f\u80fd\u5728\u7ed3\u679c\u4e0d\u4e00\u81f4\u65f6\u7f29\u5c0f\u6392\u67e5\u8303\u56f4\u3002<\/p>\n
\u5f53\u8fd9\u5957\u63d0\u53d6\u4e0e\u6838\u9a8c\u6d41\u7a0b\u9700\u8981\u8986\u76d6\u591a\u6279\u5168\u57fa\u56e0\u7ec4\u6837\u672c\u65f6\uff0c\u53ef\u901a\u8fc7\u4e91\u751f\u4fe1\u751f\u7269\u5206\u6790\u670d\u52a1\u5668\u670d\u52a1<\/a>\u7edf\u4e00\u8fd0\u884c\u73af\u5883\u4e0e\u6279\u91cf\u4efb\u52a1\u8bb0\u5f55\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u5b9e\u8dd1 MethylDackel \u7684\u91cd\u53e0\u5904\u7406\u3001\u8d28\u91cf\u8fc7\u6ee4\u3001\u91cd\u590d\u6807\u8bb0\u4e0e CpG \u94fe\u5408\u5e76\uff0c\u660e\u786e\u7f3a\u5931\u3001\u96f6\u6bd4\u4f8b\u548c\u96f6\u8986\u76d6\u7684\u533a\u522b\u3002<\/p>\n","protected":false},"author":1,"featured_media":73274,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[1429,1424,82,1423],"class_list":["post-73275","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-methyldackel","tag-wgbs","tag-82","tag-1423"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/methyldackel-overlap-quality-cpg-merge-depth-practice-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/73275","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=73275"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/73275\/revisions"}],"predecessor-version":[{"id":73280,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/73275\/revisions\/73280"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/73274"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=73275"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=73275"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=73275"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}