{"id":73277,"date":"2026-11-15T15:51:00","date_gmt":"2026-11-15T07:51:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73277"},"modified":"2026-11-15T15:51:00","modified_gmt":"2026-11-15T07:51:00","slug":"methylkit-coverage-unite-weighted-effect-pooling-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/methylkit-coverage-unite-weighted-effect-pooling-practice\/","title":{"rendered":"methylKit \u5b9e\u8dd1\u6307\u5357\u2014\u2014\u8986\u76d6\u7b5b\u9009\u3001\u52a0\u6743\u5dee\u5f02\u4e0e\u5408\u5e76\u91cd\u590d\uff0c\u4e3a\u4ec0\u4e48\u4f1a\u6539\u53d8\u7ed3\u679c"},"content":{"rendered":"
\n

\u7532\u57fa\u5316\u5dee\u5f02\u5206\u6790\u4e2d\uff0c\u4e00\u4e2a\u4f4d\u70b9\u4e3a\u4ec0\u4e48\u6d88\u5931\uff0c\u5f80\u5f80\u6bd4\u5b83\u6700\u540e\u7684\u663e\u8457\u6027\u66f4\u503c\u5f97\u5148\u95ee\u3002\u5b83\u53ef\u80fd\u5728\u8bfb\u53d6\u65f6\u5c31\u88ab\u8df3\u8fc7\uff0c\u53ef\u80fd\u5728\u67d0\u4e2a\u6837\u672c\u4e2d\u8fc7\u6ee4\u6389\uff0c\u4e5f\u53ef\u80fd\u56e0\u4e3a\u8981\u6c42\u6240\u6709\u6837\u672c\u5171\u540c\u8986\u76d6\u800c\u65e0\u6cd5\u8fdb\u5165\u68c0\u9a8c\u3002\u82e5\u53ea\u6bd4\u8f83\u6700\u7ec8\u5dee\u5f02\u4f4d\u70b9\u7684\u6570\u91cf\uff0c\u8fd9\u4e9b\u6027\u8d28\u5b8c\u5168\u4e0d\u540c\u7684\u53d8\u5316\u5f88\u5bb9\u6613\u6df7\u5728\u4e00\u8d77\u3002<\/p>\n

\u672c\u6587\u5b9e\u9645\u5b89\u88c5 methylKit 1.36.0\uff0c\u5728 R 4.5.2 \u4e2d\u5b8c\u6210\u8ba1\u6570\u8868\u8bfb\u53d6\u3001\u8986\u76d6\u7b5b\u9009\u3001\u4f4d\u70b9\u5408\u5e76\u3001\u8986\u76d6\u6807\u51c6\u5316\u3001\u5dee\u5f02\u68c0\u9a8c\u548c\u78c1\u76d8\u6570\u636e\u5e93\u64cd\u4f5c\u3002\u6570\u636e\u7531\u811a\u672c\u751f\u6210\uff0c\u5305\u62ec\u4e24\u7ec4\u4e09\u4e2a\u91cd\u590d\u3001\u4e94\u767e\u4e2a\u5019\u9009 CpG\uff0c\u4ee5\u53ca\u53ef\u8ffd\u6eaf\u7684\u7f3a\u5931\u548c\u5dee\u5f02\u8bbe\u8ba1\u3002\u8fd9\u91cc\u6ca1\u6709\u771f\u5b9e\u6d4b\u5e8f\u6837\u672c\uff0c\u4e5f\u6ca1\u6709\u8fd0\u884c\u6bd4\u5bf9\u6216\u7532\u57fa\u5316\u63d0\u53d6\uff0c\u76ee\u7684\u5728\u4e8e\u6838\u9a8c\u5206\u6790\u89c4\u5219\uff0c\u4e0d\u662f\u6bd4\u8f83\u5de5\u5177\u51c6\u786e\u7387\u3002<\/p>\n

\"\u7532\u57fa\u5316\u5dee\u5f02\uff0c\u8981\u4fdd\u7559\u91cd\u590d\u3002methylKit
methylKit 1.36.0 \u00b7 \u516d\u4e2a\u6a21\u62df\u6837\u672c\u5b8c\u6574\u5b9e\u8dd1<\/figcaption><\/figure>\n

\u4e00\u3001\u5148\u8ba9\u8ba1\u6570\u8868\u4fdd\u7559\u5b9e\u9a8c\u4fe1\u606f<\/h2>\n

methylKit \u53ef\u4ee5\u63a5\u6536\u591a\u79cd\u7532\u57fa\u5316\u7ed3\u679c\u683c\u5f0f\uff0c\u5b98\u65b9\u6559\u7a0b<\/a>\u4ecb\u7ecd\u4e86\u4ece\u8ba1\u6570\u5bfc\u5165\u5230\u5dee\u5f02\u5206\u6790\u7684\u4e3b\u8981\u6b65\u9aa4\u3002\u672c\u4f8b\u9009\u62e9 Bismark coverage \u683c\u5f0f\uff0c\u6bcf\u884c\u4fdd\u7559\u67d3\u8272\u4f53\u3001\u4f4d\u7f6e\u3001\u767e\u5206\u6bd4\u3001\u7532\u57fa\u5316\u8ba1\u6570\u548c\u975e\u7532\u57fa\u5316\u8ba1\u6570\u3002\u8fd9\u91cc\u7684\u6587\u4ef6\u7531\u6211\u4eec\u7f16\u5199\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u672c\u6b21\u8fd0\u884c\u4e86 Bismark\u3002<\/p>\n

\u516d\u4e2a\u6837\u672c\u4f9d\u6b21\u4e3a\u4e09\u4e2a\u5bf9\u7167\u548c\u4e09\u4e2a\u5904\u7406\uff0c\u5206\u7ec4\u5411\u91cf\u4e0e\u6587\u4ef6\u987a\u5e8f\u4e00\u4e00\u5bf9\u5e94\u3002\u666e\u901a\u4f4d\u70b9\u7684\u8986\u76d6\u6570\u6309\u6837\u672c\u8bbe\u7f6e\u4e0d\u540c\u500d\u6570\uff0c\u540c\u65f6\u52a0\u5165\u6837\u672c\u95f4\u6bd4\u4f8b\u6ce2\u52a8\uff0c\u518d\u6309\u8986\u76d6\u6570\u751f\u6210\u6574\u6570\u8ba1\u6570\u3002\u8fd9\u6837\uff0c\u540c\u4e00\u7ec4\u5185\u4e0d\u4f1a\u88ab\u4eba\u4e3a\u8bbe\u6210\u5b8c\u5168\u4e00\u81f4\uff0c\u540e\u7eed\u6a21\u578b\u4e5f\u6709\u5b9e\u9645\u9700\u8981\u5904\u7406\u7684\u91cd\u590d\u95f4\u53d8\u5316\u3002<\/p>\n

\u6211\u4eec\u53e6\u5916\u8bbe\u7f6e\u4e86\u51e0\u7c7b\u53ef\u6838\u5bf9\u7684\u5f02\u5e38\u3002\u5341\u4e2a\u4f4d\u70b9\u5728\u5168\u90e8\u5904\u7406\u6837\u672c\u4e2d\u7f3a\u5931\uff0c\u4e8c\u5341\u4e2a\u4f4d\u70b9\u5728\u7b2c\u4e09\u4e2a\u5bf9\u7167\u548c\u7b2c\u4e09\u4e2a\u5904\u7406\u6837\u672c\u4e2d\u7f3a\u5931\u3002\u53e6\u6709\u5341\u4e2a\u4f4d\u70b9\u5728\u7b2c\u4e8c\u4e2a\u5bf9\u7167\u4e2d\u53ea\u6709\u4e09\u6b21\u8986\u76d6\uff0c\u4ee5\u53ca\u4e00\u4e2a\u4f4d\u70b9\u5728\u7b2c\u4e00\u4e2a\u5bf9\u7167\u4e2d\u8fbe\u5230\u5341\u4e07\u6b21\u8986\u76d6\u3002\u5b83\u4eec\u7684\u4f4d\u7f6e\u4e92\u4e0d\u91cd\u53e0\uff0c\u56e0\u6b64\u6bcf\u4e00\u6b65\u51cf\u5c11\u591a\u5c11\u884c\u90fd\u53ef\u4ee5\u72ec\u7acb\u7b97\u6e05\u3002<\/p>\n

\u8f93\u5165\u68c0\u67e5\u5e94\u5f53\u540c\u65f6\u8986\u76d6\u975e\u8d1f\u6574\u6570\u8ba1\u6570\u3001\u4f4d\u7f6e\u552f\u4e00\u6027\u548c\u5206\u7ec4\u5bf9\u5e94\u5173\u7cfb\u3002\u6837\u672c\u5217\u9519\u4f4d\u53ef\u80fd\u4e0d\u4f1a\u8ba9\u6a21\u578b\u62a5\u9519\uff0c\u5374\u4f1a\u6539\u53d8\u7814\u7a76\u95ee\u9898\u3002\u767e\u5206\u6bd4\u8fd8\u5e94\u4e0e\u4e24\u79cd\u8ba1\u6570\u4e4b\u548c\u5339\u914d\uff0c\u4e0d\u80fd\u4e00\u8fb9\u4f7f\u7528\u67d0\u5957\u8fc7\u6ee4\u540e\u7684\u767e\u5206\u6bd4\uff0c\u4e00\u8fb9\u642d\u914d\u53e6\u4e00\u5957\u672a\u8fc7\u6ee4\u8ba1\u6570\u3002\u4fdd\u7559\u539f\u59cb\u63d0\u53d6\u6587\u4ef6\u4e0e\u5bfc\u5165\u53c2\u6570\uff0c\u901a\u5e38\u6bd4\u4e8b\u540e\u8ffd\u67e5\u4e00\u5f20\u6700\u7ec8\u7ed3\u679c\u8868\u66f4\u7701\u529b\u3002<\/p>\n

\u4e8c\u3001\u8fd9\u6b21\u771f\u6b63\u9047\u5230\u7684\u95ee\u9898\u662f\u9996\u884c\u88ab\u8df3\u8fc7<\/h2>\n

\u6700\u521d\u8bfb\u53d6\u65e0\u8868\u5934\u6587\u4ef6\u65f6\uff0c\u6211\u4eec\u6ca1\u6709\u663e\u5f0f\u6307\u5b9a\u662f\u5426\u5b58\u5728\u8868\u5934\u3002\u7a0b\u5e8f\u80fd\u591f\u5b8c\u6210\u8bfb\u53d6\uff0c\u4f46\u516d\u4e2a\u6587\u4ef6\u5404\u5c11\u4e86\u4e00\u884c\uff0c\u9996\u4e2a\u5750\u6807\u5728\u6240\u6709\u6837\u672c\u4e2d\u6d88\u5931\u3002\u8fd9\u4e2a\u9519\u8bef\u6ca1\u6709\u8868\u73b0\u4e3a\u65e0\u6cd5\u89e3\u6790\u6587\u4ef6\uff0c\u53cd\u800c\u4ea7\u751f\u4e86\u770b\u8d77\u6765\u6b63\u5e38\u7684\u5bf9\u8c61\uff0c\u968f\u540e\u8986\u76d6\u6570\u6838\u5bf9\u624d\u66b4\u9732\u51fa\u4e0d\u4e00\u81f4\u3002<\/p>\n

\u663e\u5f0f\u8bbe\u5b9a\u6ca1\u6709\u8868\u5934\u540e\uff0c\u516d\u4e2a\u6587\u4ef6\u5206\u522b\u8bfb\u5165\u4e94\u767e\u3001\u4e94\u767e\u3001\u56db\u767e\u516b\u5341\u3001\u56db\u767e\u4e5d\u5341\u3001\u56db\u767e\u4e5d\u5341\u548c\u56db\u767e\u4e03\u5341\u884c\u3002\u6211\u4eec\u53c8\u5c06\u9ed8\u8ba4\u8bfb\u53d6\u4e0e\u660e\u786e\u65e0\u8868\u5934\u8bfb\u53d6\u4f5c\u4e3a\u4e24\u6b21\u72ec\u7acb\u5b9e\u9a8c\uff0c\u786e\u8ba4\u5dee\u5f02\u6b63\u597d\u662f\u6bcf\u4e2a\u6587\u4ef6\u7684\u7b2c\u4e00\u884c\u3002\u8fd9\u4e2a\u7ec6\u8282\u8bf4\u660e\uff0c\u6587\u4ef6\u683c\u5f0f\u540d\u79f0\u4e0d\u80fd\u4ee3\u66ff\u6240\u6709\u5bfc\u5165\u53c2\u6570\u3002<\/p>\n

raw <- methRead(\r\n  as.list(files),\r\n  sample.id = as.list(ids),\r\n  assembly = "toy",\r\n  treatment = c(0, 0, 0, 1, 1, 1),\r\n  context = "CpG",\r\n  pipeline = "bismarkCoverage",\r\n  header = FALSE,\r\n  mincov = 1\r\n)\r\n<\/code><\/pre>\n
\u4e0a\u9762\u7684\u4f4e\u8bfb\u53d6\u95e8\u69db\u53ea\u662f\u4e3a\u4e86\u4fdd\u7559\u672c\u4f8b\u6545\u610f\u8bbe\u7f6e\u7684\u4f4e\u8986\u76d6\u8bb0\u5f55\uff0c\u4fbf\u4e8e\u89c2\u5bdf\u4e0b\u4e00\u6b65\u7b5b\u9009\u3002\u771f\u5b9e\u4efb\u52a1\u4e2d\uff0c\u8bfb\u53d6\u9636\u6bb5\u5df2\u7ecf\u5254\u9664\u7684\u4f4d\u70b9\u4e0d\u4f1a\u5728\u540e\u7eed\u653e\u5bbd\u53c2\u6570\u65f6\u81ea\u52a8\u56de\u6765\u3002\u56e0\u6b64\uff0c\u62a5\u544a\u8986\u76d6\u8fc7\u6ee4\u89c4\u5219\u65f6\uff0c\u65e2\u8981\u8bb0\u5f55\u5bfc\u5165\u95e8\u69db\uff0c\u4e5f\u8981\u8bb0\u5f55\u4e4b\u540e\u7684\u663e\u5f0f\u7b5b\u9009\uff0c\u4e0d\u80fd\u53ea\u5199\u6700\u540e\u4e00\u4e2a\u53c2\u6570\u3002<\/p>\n
\u4e09\u3001\u9010\u6837\u672c\u8fc7\u6ee4\u4e0e\u5171\u540c\u4f4d\u70b9\u7b5b\u9009\u8981\u5206\u5f00\u7edf\u8ba1<\/h2>\n
\u672c\u4f8b\u4f7f\u7528\u6700\u4f4e\u8986\u76d6\u5341\u3001\u6700\u9ad8\u8986\u76d6\u4e94\u767e\u8fdb\u884c\u7b5b\u9009\u3002\u7b2c\u4e00\u4e2a\u5bf9\u7167\u56e0\u6781\u7aef\u9ad8\u8986\u76d6\u51cf\u5c11\u4e00\u884c\uff0c\u7b2c\u4e8c\u4e2a\u5bf9\u7167\u56e0\u4f4e\u8986\u76d6\u51cf\u5c11\u5341\u884c\uff0c\u5176\u4f59\u6837\u672c\u4e0d\u53d8\u3002\u8fd9\u4e00\u6b65\u662f\u5728\u5404\u6837\u672c\u5185\u90e8\u5b8c\u6210\u7684\uff0c\u8fd8\u6ca1\u6709\u8981\u6c42\u67d0\u4e2a\u4f4d\u70b9\u5728\u5176\u4ed6\u6837\u672c\u4e2d\u5b58\u5728\u3002<\/p>\n
filt <- filterByCoverage(raw, lo.count = 10, hi.count = 500)\r\nstrict <- unite(filt, destrand = FALSE)\r\nrelaxed <- unite(filt, destrand = FALSE, min.per.group = 2L)\r\n<\/code><\/pre>\n
\u9ed8\u8ba4\u8981\u6c42\u6240\u6709\u516d\u4e2a\u6837\u672c\u5171\u540c\u8986\u76d6\u65f6\uff0c\u6700\u7ec8\u7559\u4e0b\u56db\u767e\u4e94\u5341\u4e5d\u4e2a\u4f4d\u70b9\u3002\u8ba1\u7b97\u8fc7\u7a0b\u662f\u4ece\u4e94\u767e\u4e2a\u5019\u9009\u4e2d\uff0c\u53bb\u6389\u6574\u7ec4\u7f3a\u5931\u7684\u5341\u4e2a\u3001\u4e24\u4e2a\u6837\u672c\u7f3a\u5931\u7684\u4e8c\u5341\u4e2a\u3001\u4e00\u4e2a\u6837\u672c\u4f4e\u8986\u76d6\u7684\u5341\u4e2a\uff0c\u4ee5\u53ca\u4e00\u4e2a\u6837\u672c\u6781\u9ad8\u8986\u76d6\u7684\u4e00\u4e2a\u3002\u8fd9\u4e0d\u662f\u4e94\u767e\u51cf\u53bb\u6240\u6709\u6837\u672c\u635f\u5931\u884c\u6570\u7684\u7b80\u5355\u603b\u548c\uff0c\u56e0\u4e3a\u540c\u4e00\u4f4d\u7f6e\u53ef\u80fd\u5728\u591a\u4e2a\u6837\u672c\u4e2d\u7f3a\u5931\u3002<\/p>\n
\n\n\n\n\n\n\n\n\n
\u5904\u7406\u9636\u6bb5<\/th>\n\u4fdd\u7559\u6570\u91cf<\/th>\n\u5b9e\u9645\u542b\u4e49<\/th>\n<\/tr>\n<\/thead>\n
\u7b2c\u4e00\u4e2a\u5bf9\u7167\u8fc7\u6ee4\u540e<\/td>\n499<\/td>\n\u53bb\u6389\u4e00\u4e2a\u6781\u9ad8\u8986\u76d6\u4f4d\u7f6e<\/td>\n<\/tr>\n
\u7b2c\u4e8c\u4e2a\u5bf9\u7167\u8fc7\u6ee4\u540e<\/td>\n490<\/td>\n\u53bb\u6389\u5341\u4e2a\u4f4e\u8986\u76d6\u4f4d\u7f6e<\/td>\n<\/tr>\n
\u8981\u6c42\u516d\u4e2a\u6837\u672c\u5747\u8986\u76d6<\/td>\n459<\/td>\n\u8fdb\u5165\u4e25\u683c\u5171\u540c\u96c6\u5408<\/td>\n<\/tr>\n
\u6bcf\u7ec4\u81f3\u5c11\u4e24\u4e2a\u6837\u672c\u8986\u76d6<\/td>\n490<\/td>\n\u5141\u8bb8\u90e8\u5206\u6837\u672c\u4fdd\u7559\u7f3a\u5931\u503c<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u6bcf\u7ec4\u81f3\u5c11\u4e24\u4e2a\u6837\u672c\u8986\u76d6\u65f6\uff0c\u53ea\u6709\u5168\u90e8\u5904\u7406\u6837\u672c\u7f3a\u5931\u7684\u5341\u4e2a\u4f4d\u70b9\u65e0\u6cd5\u8fdb\u5165\uff0c\u6700\u7ec8\u7559\u4e0b\u56db\u767e\u4e5d\u5341\u4e2a\u4f4d\u7f6e\u3002\u5176\u4ed6\u4f4d\u7f6e\u4e2d\u7f3a\u5931\u7684\u6837\u672c\u4ecd\u7136\u662f\u7f3a\u5931\u503c\uff0c\u5e76\u6ca1\u6709\u8865\u6210\u96f6\u7532\u57fa\u5316\u3002\u8fd9\u4e2a\u533a\u522b\u76f4\u63a5\u5f71\u54cd\u89e3\u91ca\uff0c\u56e0\u4e3a\u6ca1\u6709\u8bfb\u6bb5\u8bc1\u636e\u4e0e\u89c2\u5bdf\u5230\u5168\u90e8\u672a\u7532\u57fa\u5316\u7684\u8bfb\u6bb5\uff0c\u5e76\u4e0d\u662f\u540c\u4e00\u79cd\u6570\u636e\u3002<\/p>\n
\u653e\u5bbd\u5171\u540c\u8986\u76d6\u8981\u6c42\u53ef\u4ee5\u4fdd\u7559\u66f4\u591a\u4f4d\u7f6e\uff0c\u4f46\u4e0d\u540c\u4f4d\u70b9\u4f7f\u7528\u7684\u6709\u6548\u6837\u672c\u6570\u4e5f\u4f1a\u4e0d\u540c\u3002\u771f\u5b9e\u9879\u76ee\u5e94\u68c0\u67e5\u7f3a\u5931\u662f\u5426\u96c6\u4e2d\u4e8e\u67d0\u7ec4\u3001\u67d0\u6279\u6b21\u6216\u7279\u5b9a\u533a\u57df\u3002\u82e5\u7f3a\u5931\u672c\u8eab\u4e0e\u5904\u7406\u6709\u5173\uff0c\u589e\u52a0\u4fdd\u7559\u6570\u91cf\u5e76\u4e0d\u80fd\u6d88\u9664\u9009\u62e9\u504f\u5dee\u3002\u672c\u6587\u540e\u7eed\u4e3b\u8981\u68c0\u9a8c\u4f7f\u7528\u4e25\u683c\u96c6\u5408\uff0c\u907f\u514d\u628a\u4e0d\u540c\u4f4d\u70b9\u6837\u672c\u6570\u53d8\u5316\u4e0e\u5176\u4ed6\u5b9e\u9a8c\u540c\u65f6\u6df7\u5408\u3002<\/p>\n
\u56db\u3001\u8986\u76d6\u6807\u51c6\u5316\u4e0d\u662f\u8ba9\u7532\u57fa\u5316\u6bd4\u4f8b\u53d8\u5f97\u76f8\u540c<\/h2>\n
\u6211\u4eec\u5bf9\u8fc7\u6ee4\u540e\u7684\u516d\u4e2a\u6837\u672c\u72ec\u7acb\u8fd0\u884c\u4e2d\u4f4d\u6570\u8986\u76d6\u6807\u51c6\u5316\u3002\u5904\u7406\u524d\u7684\u4e2d\u4f4d\u8986\u76d6\u4f9d\u6b21\u4e3a\u4e09\u5341\u3001\u4e94\u5341\u516b\u3001\u4e00\u767e\u4e8c\u5341\u3001\u4e09\u5341\u3001\u516d\u5341\u4e00\u548c\u4e00\u767e\u4e00\u5341\u516d\uff0c\u5904\u7406\u540e\u5747\u53d8\u4e3a\u4e00\u767e\u4e8c\u5341\u3002\u8fd9\u4e2a\u64cd\u4f5c\u6539\u53d8\u7684\u662f\u8ba1\u6570\u7684\u5c3a\u5ea6\uff0c\u5e76\u4e0d\u8981\u6c42\u6bcf\u4e2a\u6837\u672c\u7684\u7532\u57fa\u5316\u6bd4\u4f8b\u5206\u5e03\u4e00\u81f4\u3002<\/p>\n
\u7b2c\u4e00\u4e2a\u5bf9\u7167\u7684\u793a\u4f8b\u4f4d\u70b9\u539f\u6765\u662f\u5341\u6b21\u8986\u76d6\uff0c\u5176\u4e2d\u4e00\u6b21\u7532\u57fa\u5316\u3002\u6807\u51c6\u5316\u540e\u53d8\u6210\u56db\u5341\u6b21\u8986\u76d6\u3001\u56db\u6b21\u7532\u57fa\u5316\uff0c\u6bd4\u4f8b\u4ecd\u4e3a\u767e\u5206\u4e4b\u5341\u3002\u7b2c\u4e8c\u4e2a\u5bf9\u7167\u7684\u540c\u4e00\u4f4d\u70b9\u539f\u6765\u662f\u767e\u5206\u4e4b\u4e5d\u5341\uff0c\u7ecf\u8fc7\u975e\u6574\u6570\u500d\u7f29\u653e\u548c\u53d6\u6574\u540e\uff0c\u8986\u76d6\u4e3a\u4e8c\u767e\u96f6\u4e03\u3001\u7532\u57fa\u5316\u8ba1\u6570\u4e3a\u4e00\u767e\u516b\u5341\u516d\uff0c\u6bd4\u4f8b\u51fa\u73b0\u5c0f\u5e45\u53d8\u5316\u3002<\/p>\n
\u672c\u6b21\u6700\u5927\u6bd4\u4f8b\u53d8\u5316\u4e0d\u5230\u4e00\u4e2a\u767e\u5206\u70b9\uff0c\u6765\u81ea\u8ba1\u6570\u53d6\u6574\u3002\u8fd8\u6709\u4e2a\u522b\u4f4d\u7f6e\u7684\u603b\u8986\u76d6\u4e0e\u4e24\u7c7b\u8ba1\u6570\u5206\u522b\u53d6\u6574\u540e\u7684\u548c\u76f8\u5dee\u4e00\u3002\u8fd9\u63d0\u9192\u6211\u4eec\uff0c\u6807\u51c6\u5316\u540e\u7684\u6574\u6570\u662f\u7528\u4e8e\u5206\u6790\u7684\u53d8\u6362\u7ed3\u679c\uff0c\u4e0d\u5e94\u91cd\u65b0\u5305\u88c5\u6210\u539f\u59cb\u6d4b\u5e8f\u5206\u5b50\u6570\uff0c\u4e5f\u4e0d\u5b9c\u4e0e\u672a\u7ecf\u53d8\u6362\u7684\u8ba1\u6570\u6df7\u5408\u540e\u518d\u9001\u5165\u53e6\u4e00\u5957\u6d41\u7a0b\u3002<\/p>\n
\u66f4\u5173\u952e\u7684\u662f\uff0c\u6807\u51c6\u5316\u4e0d\u80fd\u589e\u52a0\u5b9e\u9a8c\u89c2\u6d4b\uff0c\u4e5f\u4e0d\u80fd\u5236\u9020\u751f\u7269\u5b66\u91cd\u590d\u3002\u672c\u6587\u5c06\u5b83\u4f5c\u4e3a\u72ec\u7acb\u6f14\u793a\uff0c\u540e\u9762\u7684\u5dee\u5f02\u68c0\u9a8c\u4ecd\u4f7f\u7528\u672a\u505a\u6b64\u7f29\u653e\u7684\u4e25\u683c\u5171\u540c\u96c6\u5408\u3002\u82e5\u81ea\u5df1\u7684\u6d41\u7a0b\u91c7\u7528\u6807\u51c6\u5316\uff0c\u5e94\u8be5\u660e\u786e\u8bb0\u5f55\u5b83\u53d1\u751f\u5728\u4ec0\u4e48\u9636\u6bb5\uff0c\u5e76\u5728\u5408\u7406\u8303\u56f4\u5185\u68c0\u67e5\u7ed3\u679c\u5bf9\u8be5\u9009\u62e9\u662f\u5426\u654f\u611f\u3002<\/p>\n
\u4e94\u3001\u52a0\u6743\u5747\u503c\u4e0e\u6837\u672c\u5747\u503c\u56de\u7b54\u7684\u95ee\u9898\u4e0d\u540c<\/h2>\n
\u6211\u4eec\u4e13\u95e8\u8bbe\u7f6e\u4e00\u4e2a\u5f02\u8d28\u4f4d\u70b9\u3002\u4e09\u4e2a\u5bf9\u7167\u7684\u7532\u57fa\u5316\u8ba1\u6570\u4e0e\u603b\u8986\u76d6\u5206\u522b\u4e3a\u4e00\u9664\u4ee5\u5341\u3001\u4e5d\u5341\u9664\u4ee5\u4e00\u767e\u3001\u4e5d\u5341\u9664\u4ee5\u4e00\u767e\uff0c\u4e09\u4e2a\u5904\u7406\u5219\u5206\u522b\u4e3a\u4e94\u9664\u4ee5\u5341\u3001\u4e94\u5341\u9664\u4ee5\u4e00\u767e\u3001\u4e94\u5341\u9664\u4ee5\u4e00\u767e\u3002\u5904\u7406\u7ec4\u5728\u6bcf\u4e2a\u6837\u672c\u4e2d\u90fd\u662f\u767e\u5206\u4e4b\u4e94\u5341\uff0c\u5bf9\u7167\u7ec4\u5219\u662f\u767e\u5206\u4e4b\u5341\u3001\u4e5d\u5341\u548c\u4e5d\u5341\u3002<\/p>\n
\u82e5\u6309\u8986\u76d6\u52a0\u6743\uff0c\u5bf9\u7167\u7ec4\u662f\u603b\u7532\u57fa\u5316\u8ba1\u6570\u4e00\u767e\u516b\u5341\u4e00\u9664\u4ee5\u603b\u8986\u76d6\u4e8c\u767e\u4e00\u5341\uff0c\u7ea6\u4e3a\u767e\u5206\u4e4b\u516b\u5341\u516d\u70b9\u4e00\u4e5d\u3002\u5904\u7406\u51cf\u5bf9\u7167\u5f97\u5230\u8d1f\u4e09\u5341\u516d\u70b9\u4e00\u4e5d\u4e2a\u767e\u5206\u70b9\u3002\u5982\u679c\u5148\u7b97\u6bcf\u4e2a\u6837\u672c\u6bd4\u4f8b\uff0c\u518d\u5bf9\u4e09\u4e2a\u6837\u672c\u53d6\u666e\u901a\u5e73\u5747\uff0c\u5bf9\u7167\u7ec4\u7ea6\u4e3a\u767e\u5206\u4e4b\u516d\u5341\u4e09\u70b9\u4e09\u4e09\uff0c\u5dee\u5f02\u5c31\u6210\u4e3a\u8d1f\u5341\u4e09\u70b9\u4e09\u4e09\u4e2a\u767e\u5206\u70b9\u3002<\/p>\n
weighted <- calculateDiffMeth(\r\n  strict, overdispersion = "MN",\r\n  test = "F", adjust = "BH", effect = "wmean", mc.cores = 1\r\n)\r\naverage <- calculateDiffMeth(\r\n  strict, overdispersion = "MN",\r\n  test = "F", adjust = "BH", effect = "mean", mc.cores = 1\r\n)\r\n<\/code><\/pre>\n
\u4e24\u6b21\u8fd0\u884c\u7684 P \u503c\u548c\u6821\u6b63\u503c\u9010\u9879\u76f8\u540c\uff0c\u6539\u53d8\u7684\u662f\u62a5\u544a\u7684\u5dee\u5f02\u5e45\u5ea6\u3002\u5bf9\u4e8e\u8fd9\u4e2a\u4f4d\u70b9\uff0c\u6821\u6b63\u503c\u7ea6\u4e3a\u96f6\u70b9\u4e09\u516b\u56db\uff0c\u4e24\u79cd\u6548\u5e94\u5b9a\u4e49\u90fd\u6ca1\u6709\u901a\u8fc7\u672c\u4f8b\u7684\u663e\u8457\u6027\u95e8\u69db\u3002\u4e0d\u80fd\u56e0\u4e3a\u52a0\u6743\u5dee\u5f02\u8d85\u8fc7\u4e8c\u5341\u4e94\u4e2a\u767e\u5206\u70b9\uff0c\u5c31\u628a\u5b83\u5355\u72ec\u8ba4\u5b9a\u4e3a\u53ef\u9760\u5dee\u5f02\uff0c\u66f4\u4e0d\u80fd\u5c06\u6548\u5e94\u5b9a\u4e49\u53d8\u5316\u8bef\u5199\u6210\u7edf\u8ba1\u6a21\u578b\u91cd\u65b0\u8bc1\u660e\u4e86\u5dee\u5f02\u3002<\/p>\n
\u767e\u5206\u6bd4\u4e4b\u5dee\u7684\u5355\u4f4d\u662f\u767e\u5206\u70b9\u3002\u7531\u767e\u5206\u4e4b\u4e8c\u5341\u53d8\u4e3a\u767e\u5206\u4e4b\u4e09\u5341\uff0c\u5dee\u5341\u4e2a\u767e\u5206\u70b9\uff0c\u4e0e\u76f8\u5bf9\u589e\u52a0\u767e\u5206\u4e4b\u4e94\u5341\u662f\u4e24\u79cd\u8868\u8fbe\u3002\u8bbe\u7f6e\u5dee\u5f02\u5e45\u5ea6\u9608\u503c\u65f6\u5fc5\u987b\u786e\u8ba4\u8f6f\u4ef6\u4f7f\u7528\u54ea\u4e00\u79cd\u3002\u5b9e\u9645\u62a5\u544a\u4e5f\u5e94\u8bf4\u660e\u5904\u7406\u51cf\u5bf9\u7167\u7684\u65b9\u5411\uff0c\u907f\u514d\u8bfb\u8005\u628a\u8d1f\u53f7\u89e3\u91ca\u6210\u76f8\u53cd\u7684\u751f\u7269\u5b66\u53d8\u5316\u3002<\/p>\n
\u516d\u3001\u5148\u5408\u5e76\u91cd\u590d\uff0c\u4f1a\u4e22\u5931\u7ec4\u5185\u6ce2\u52a8\u4fe1\u606f<\/h2>\n
\u63a5\u7740\u6211\u4eec\u5c06\u6bcf\u7ec4\u7684\u4e09\u4e2a\u91cd\u590d\u5408\u5e76\u8ba1\u6570\uff0c\u518d\u8fd0\u884c\u9002\u5408\u6bcf\u7ec4\u4e00\u4e2a\u5408\u5e76\u6837\u672c\u7684 Fisher \u68c0\u9a8c\u3002\u5f02\u8d28\u4f4d\u70b9\u7684\u52a0\u6743\u5dee\u5f02\u4ecd\u7136\u662f\u8d1f\u4e09\u5341\u516d\u70b9\u4e00\u4e5d\u4e2a\u767e\u5206\u70b9\uff0c\u4f46\u6821\u6b63\u503c\u4ece\u7ea6\u96f6\u70b9\u4e09\u516b\u56db\u53d8\u6210\u7ea6\u516b\u4e58\u5341\u7684\u8d1f\u5341\u4e94\u6b21\u65b9\u3002\u5dee\u5f02\u5e45\u5ea6\u6ca1\u6709\u6539\u53d8\uff0c\u7edf\u8ba1\u8bc1\u636e\u5374\u770b\u8d77\u6765\u5f3a\u4e86\u8bb8\u591a\u3002<\/p>\n
\u539f\u56e0\u53ef\u4ee5\u76f4\u63a5\u4ece\u8f93\u5165\u7406\u89e3\u3002\u5408\u5e76\u540e\u53ea\u5269\u5bf9\u7167\u7ec4\u4e00\u767e\u516b\u5341\u4e00\u6bd4\u4e8c\u5341\u4e5d\u3001\u5904\u7406\u7ec4\u4e00\u767e\u96f6\u4e94\u6bd4\u4e00\u767e\u96f6\u4e94\u8fd9\u4e24\u7ec4\u8ba1\u6570\uff0c\u539f\u6765\u5bf9\u7167\u6837\u672c\u4e4b\u95f4\u767e\u5206\u4e4b\u5341\u4e0e\u767e\u5206\u4e4b\u4e5d\u5341\u7684\u5dee\u522b\u5df2\u7ecf\u770b\u4e0d\u89c1\u3002\u6a21\u578b\u83b7\u5f97\u4e86\u66f4\u591a\u6c47\u603b\u8bfb\u6bb5\uff0c\u5374\u5931\u53bb\u4e86\u54ea\u4e9b\u8bfb\u6bb5\u6765\u81ea\u54ea\u4e2a\u751f\u7269\u5b66\u91cd\u590d\u7684\u4fe1\u606f\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u5b9e\u9645\u8fd0\u884c\u65b9\u5f0f<\/th>\n\u5dee\u5f02\u5e45\u5ea6<\/th>\n\u5f02\u8d28\u4f4d\u70b9\u7684\u6821\u6b63\u503c<\/th>\n<\/tr>\n<\/thead>\n
\u4fdd\u7559\u91cd\u590d\uff0c\u8986\u76d6\u52a0\u6743\u6548\u5e94<\/td>\n\u221236.19 \u4e2a\u767e\u5206\u70b9<\/td>\n\u7ea6 0.384<\/td>\n<\/tr>\n
\u4fdd\u7559\u91cd\u590d\uff0c\u6837\u672c\u5747\u503c\u6548\u5e94<\/td>\n\u221213.33 \u4e2a\u767e\u5206\u70b9<\/td>\n\u7ea6 0.384<\/td>\n<\/tr>\n
\u5408\u5e76\u91cd\u590d\u540e Fisher \u68c0\u9a8c<\/td>\n\u221236.19 \u4e2a\u767e\u5206\u70b9<\/td>\n\u7ea6 8.08 \u00d7 10\u207b\u00b9\u2075<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u5728\u672c\u4f8b\u9884\u8bbe\u7684\u516d\u5341\u4e2a\u5927\u5e45\u53d8\u5316\u4f4d\u70b9\u4e2d\uff0c\u4fdd\u7559\u91cd\u590d\u7684\u6d41\u7a0b\u6309\u6821\u6b63\u503c\u5c0f\u4e8e\u96f6\u70b9\u96f6\u4e94\u4e14\u7edd\u5bf9\u5dee\u5f02\u8d85\u8fc7\u4e8c\u5341\u4e94\u4e2a\u767e\u5206\u70b9\u7b5b\u51fa\u4e94\u5341\u516b\u4e2a\u3002\u5408\u5e76\u6d41\u7a0b\u9009\u51fa\u516d\u5341\u4e2a\u9884\u8bbe\u4f4d\u7f6e\uff0c\u8fd8\u989d\u5916\u9009\u4e2d\u4e0a\u8ff0\u5f02\u8d28\u4f4d\u70b9\u3002\u8fd9\u4e9b\u6570\u91cf\u53ea\u63cf\u8ff0\u672c\u6b21\u6784\u9020\u6570\u636e\uff0c\u4e0d\u80fd\u636e\u6b64\u8ba4\u5b9a\u67d0\u79cd\u65b9\u6cd5\u666e\u904d\u66f4\u654f\u611f\u6216\u66f4\u51c6\u786e\u3002<\/p>\n
\u5408\u5e76\u6280\u672f\u91cd\u590d\u4e0e\u5408\u5e76\u72ec\u7acb\u751f\u7269\u5b66\u91cd\u590d\u4e5f\u9700\u8981\u533a\u522b\u3002\u524d\u8005\u662f\u5426\u5408\u7406\uff0c\u5e94\u7ed3\u5408\u5b9e\u9a8c\u8bbe\u8ba1\u4e0e\u6587\u5e93\u5173\u7cfb\u5224\u65ad\uff0c\u540e\u8005\u4f1a\u76f4\u63a5\u538b\u7f29\u7ec4\u5185\u53d8\u5f02\u4fe1\u606f\u3002\u5206\u6790\u65f6\u4e0d\u5e94\u4e3a\u4e86\u589e\u52a0\u663e\u8457\u4f4d\u70b9\u6570\u91cf\u800c\u628a\u72ec\u7acb\u91cd\u590d\u63d0\u524d\u5408\u6210\u4e00\u4e2a\u6837\u672c\u3002\u66f4\u6709\u4ef7\u503c\u7684\u505a\u6cd5\u662f\u4fdd\u7559\u91cd\u590d\u8eab\u4efd\uff0c\u5e76\u68c0\u67e5\u79bb\u7fa4\u6837\u672c\u3001\u6279\u6b21\u53ca\u6a21\u578b\u5047\u8bbe\u3002<\/p>\n
\u4e03\u3001\u78c1\u76d8\u6570\u636e\u5e93\u6a21\u5f0f\u6539\u53d8\u5b58\u50a8\u65b9\u5f0f\uff0c\u4e0d\u66ff\u4ee3\u7ed3\u679c\u6838\u9a8c<\/h2>\n
\u6211\u4eec\u8fd8\u628a\u540c\u4e00\u6279\u8f93\u5165\u5199\u4e3a tabix \u652f\u6301\u7684\u78c1\u76d8\u5bf9\u8c61\uff0c\u518d\u6267\u884c\u76f8\u540c\u8fc7\u6ee4\u4e0e\u5171\u540c\u4f4d\u70b9\u5408\u5e76\u3002\u7ed3\u679c\u5f97\u5230\u56db\u767e\u4e94\u5341\u4e5d\u884c\uff0c\u9010\u5217\u4e0e\u5185\u5b58\u6a21\u5f0f\u4e00\u81f4\u3002\u8fd9\u4e2a\u68c0\u67e5\u8bc1\u660e\u672c\u6b21\u4e24\u6761\u5904\u7406\u8def\u5f84\u5728\u7ed9\u5b9a\u8f93\u5165\u548c\u53c2\u6570\u4e0b\u4ea7\u751f\u76f8\u540c\u6570\u636e\uff0c\u800c\u4e0d\u662f\u8bc1\u660e\u6570\u636e\u5e93\u6a21\u5f0f\u5728\u4efb\u4f55\u89c4\u6a21\u4e0b\u90fd\u66f4\u5feb\u3002<\/p>\n
\u78c1\u76d8\u65b9\u5f0f\u9002\u5408\u5728\u6570\u636e\u89c4\u6a21\u8f83\u5927\u65f6\u6309\u5757\u5904\u7406\uff0c\u4f46\u538b\u7f29\u6587\u4ef6\u3001\u7d22\u5f15\u548c\u5bf9\u8c61\u5f15\u7528\u9700\u8981\u4e00\u8d77\u7ba1\u7406\u3002\u5982\u679c\u53ea\u590d\u5236\u5bf9\u8c61\u800c\u6ca1\u6709\u4fdd\u7559\u5b83\u5f15\u7528\u7684\u6570\u636e\u6587\u4ef6\uff0c\u6362\u4e00\u4e2a\u76ee\u5f55\u6216\u73af\u5883\u540e\u53ef\u80fd\u65e0\u6cd5\u7ee7\u7eed\u4f7f\u7528\u3002\u5f52\u6863\u65f6\u5e94\u628a\u8fd9\u4e9b\u4f9d\u8d56\u4f5c\u4e3a\u540c\u4e00\u4e2a\u5206\u6790\u4ea7\u7269\u4fdd\u5b58\uff0c\u5e76\u7528\u4e00\u6b21\u5b9e\u9645\u8bfb\u53d6\u68c0\u67e5\u5f52\u6863\u5b8c\u6574\u6027\u3002<\/p>\n
\u6bcf\u6b21\u6f14\u793a\u4f7f\u7528\u65b0\u76ee\u5f55\u4e5f\u6709\u610f\u4e49\u3002\u6cbf\u7528\u65e7\u6570\u636e\u5e93\u6587\u4ef6\u540d\u53ef\u80fd\u8ba9\u4eba\u96be\u4ee5\u5206\u8fa8\u8bfb\u53d6\u7684\u662f\u672c\u6b21\u751f\u6210\u7684\u6570\u636e\u8fd8\u662f\u5df2\u6709\u6587\u4ef6\u3002\u6211\u4eec\u4fdd\u5b58\u4e86\u8f93\u51fa\u6587\u4ef6\u6e05\u5355\uff0c\u4fbf\u4e8e\u6838\u5bf9\u5b9e\u9645\u4ea7\u751f\u7684\u538b\u7f29\u6587\u4ef6\u4e0e\u7d22\u5f15\u3002\u8fd9\u4e2a\u4f8b\u5b50\u89c4\u6a21\u5f88\u5c0f\uff0c\u6ca1\u6709\u6d4b\u91cf\u5185\u5b58\u5cf0\u503c\u6216\u8005\u541e\u5410\u91cf\uff0c\u56e0\u6b64\u4e0d\u628a\u5b58\u50a8\u6a21\u5f0f\u7684\u9009\u62e9\u5199\u6210\u672a\u7ecf\u6d4b\u91cf\u7684\u6027\u80fd\u7ed3\u8bba\u3002<\/p>\n
\u516b\u3001\u5de5\u5177\u7684\u4f18\u52bf\u4e0e\u4f7f\u7528\u4ee3\u4ef7<\/h2>\n
methylKit \u7684\u4f18\u52bf\u662f\u4ece\u6837\u672c\u8ba1\u6570\u3001\u8986\u76d6\u8fc7\u6ee4\u5230\u5dee\u5f02\u68c0\u9a8c\u63d0\u4f9b\u8fde\u8d2f\u7684\u6570\u636e\u7ed3\u6784\uff0c\u4e5f\u5141\u8bb8\u4fdd\u7559\u91cd\u590d\u3001\u52a0\u5165\u534f\u53d8\u91cf\u5e76\u4f7f\u7528\u78c1\u76d8\u5bf9\u8c61\u3002\u5bf9\u9700\u8981\u9010\u6b65\u5ba1\u9605\u7532\u57fa\u5316\u6d41\u7a0b\u7684\u8bfb\u8005\uff0c\u8fd9\u79cd\u660e\u786e\u7684\u4e2d\u95f4\u5bf9\u8c61\u5f88\u6709\u5e2e\u52a9\u3002\u5b83\u7684\u4f7f\u7528\u4ee3\u4ef7\uff0c\u662f\u5f88\u591a\u770b\u4f3c\u7b80\u5355\u7684\u53c2\u6570\u5176\u5b9e\u4f1a\u6539\u53d8\u5206\u6790\u96c6\u5408\u6216\u8005\u6548\u5e94\u542b\u4e49\u3002<\/p>\n
\u771f\u5b9e\u9879\u76ee\u4e2d\uff0c\u8986\u76d6\u95e8\u69db\u3001\u5171\u540c\u4f4d\u70b9\u89c4\u5219\u3001\u5dee\u5f02\u5e45\u5ea6\u5b9a\u4e49\u548c\u591a\u91cd\u68c0\u9a8c\u8303\u56f4\u5e94\u5728\u5206\u6790\u8bb0\u5f55\u4e2d\u4e00\u8d77\u8bf4\u660e\u3002\u672c\u6587\u663e\u5f0f\u4f7f\u7528 BH \u6821\u6b63\uff0c\u6821\u6b63\u6240\u5bf9\u5e94\u7684\u662f\u8fdb\u5165\u672c\u6b21\u68c0\u9a8c\u7684\u56db\u767e\u4e94\u5341\u4e5d\u4e2a\u4f4d\u70b9\u3002\u82e5\u53ea\u5bfc\u51fa\u663e\u8457\u4f4d\u7f6e\u518d\u91cd\u65b0\u6821\u6b63\uff0c\u6216\u8005\u4e0d\u540c\u65b9\u6cd5\u5404\u81ea\u5728\u4e0d\u540c\u4f4d\u70b9\u96c6\u5408\u4e0a\u6821\u6b63\u540e\u76f4\u63a5\u6bd4\u8f83\u6570\u5b57\uff0c\u5f97\u5230\u7684\u7ed3\u679c\u4e0d\u518d\u56de\u7b54\u540c\u4e00\u4e2a\u95ee\u9898\u3002<\/p>\n
\u4e5d\u3001\u590d\u73b0\u540e\u6700\u5e94\u8be5\u4fdd\u7559\u54ea\u4e9b\u7ecf\u9a8c<\/h2>\n
\u4e0b\u8f7d\u672c\u7ec4\u5b8c\u6574\u811a\u672c\u4e0e\u5b9e\u8dd1\u8bb0\u5f55<\/a>\uff0c\u5728\u672c\u7bc7\u76ee\u5f55\u8fd0\u884c\u4e0b\u9762\u547d\u4ee4\uff0c\u53ef\u91cd\u65b0\u751f\u6210\u516d\u4e2a\u8ba1\u6570\u6587\u4ef6\uff0c\u5e76\u5b8c\u6210\u5185\u5b58\u548c\u78c1\u76d8\u4e24\u6761\u8def\u5f84\u3002\u811a\u672c\u56fa\u5b9a\u955c\u50cf\u6458\u8981\u4e0e\u968f\u673a\u79cd\u5b50\uff0c\u4fdd\u5b58 R \u4ee3\u7801\u3001\u7248\u672c\u3001\u8bfb\u53d6\u6570\u91cf\u3001\u7edf\u8ba1\u7ed3\u679c\u548c\u539f\u59cb\u65e5\u5fd7\u3002\u6267\u884c\u73af\u5883\u9650\u5236\u4e3a\u4e24\u6838\u3001\u56db GiB \u5185\u5b58\uff0c\u672a\u636e\u6b64\u63a8\u65ad\u771f\u5b9e\u9879\u76ee\u7684\u8d44\u6e90\u9700\u6c42\u3002<\/p>\n
python3 run.py output\r\n<\/code><\/pre>\n
\u590d\u73b0\u65f6\u5148\u6838\u5bf9\u9996\u884c\u5750\u6807\u4e0e\u8f93\u5165\u884c\u6570\uff0c\u518d\u6838\u5bf9\u9010\u6837\u672c\u8fc7\u6ee4\u6570\u91cf\u3001\u5171\u540c\u96c6\u5408\u5927\u5c0f\u548c\u793a\u4f8b\u4f4d\u70b9\u7684\u624b\u7b97\u5dee\u5f02\u3002\u6700\u540e\u518d\u770b\u663e\u8457\u4f4d\u70b9\u5217\u8868\u3002\u8fd9\u4e2a\u987a\u5e8f\u80fd\u628a\u6587\u4ef6\u8bfb\u53d6\u3001\u6837\u672c\u7f3a\u5931\u3001\u6548\u5e94\u5b9a\u4e49\u548c\u7edf\u8ba1\u63a8\u65ad\u5206\u5f00\u68c0\u67e5\uff0c\u4e5f\u80fd\u8ba9\u5206\u6790\u62a5\u544a\u89e3\u91ca\u7ed3\u679c\u4e3a\u4ec0\u4e48\u6539\u53d8\uff0c\u800c\u4e0d\u662f\u53ea\u5c55\u793a\u54ea\u6b21\u8fd0\u884c\u5f97\u5230\u4e86\u66f4\u591a\u4f4d\u70b9\u3002<\/p>\n
\u5f53\u6837\u672c\u89c4\u6a21\u6269\u5927\uff0c\u9700\u8981\u6301\u7eed\u4fdd\u5b58\u591a\u6279\u8ba1\u6570\u3001\u6a21\u578b\u4e0e\u590d\u73b0\u8bb0\u5f55\u65f6\uff0c\u53ef\u901a\u8fc7\u4e91\u751f\u4fe1\u751f\u7269\u5206\u6790\u670d\u52a1\u5668\u670d\u52a1<\/a>\u7ec4\u7ec7\u7edf\u4e00\u7684\u5206\u6790\u73af\u5883\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u5b9e\u9645\u9a8c\u8bc1 methylKit \u7684\u8868\u5934\u8bfb\u53d6\u3001\u8986\u76d6\u7b5b\u9009\u3001\u5747\u503c\u5b9a\u4e49\u3001\u91cd\u590d\u5408\u5e76\u53ca tabix \u6570\u636e\u5e93\uff0c\u89e3\u91ca\u5dee\u5f02\u4f4d\u70b9\u6570\u91cf\u4e3a\u4f55\u53d8\u5316\u3002<\/p>\n","protected":false},"author":1,"featured_media":73276,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[786,1424,1430,82],"class_list":["post-73277","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-methylkit","tag-wgbs","tag-1430","tag-82"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/methylkit-coverage-unite-weighted-effect-pooling-practice-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/73277","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=73277"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/73277\/revisions"}],"predecessor-version":[{"id":73281,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/73277\/revisions\/73281"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/73276"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=73277"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=73277"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=73277"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}