{"id":73296,"date":"2026-11-15T16:05:22","date_gmt":"2026-11-15T08:05:22","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=73296"},"modified":"2026-11-15T16:05:22","modified_gmt":"2026-11-15T08:05:22","slug":"juicer-tools-pre-mapq-intrafrag-weighted-counts-practice","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/juicer-tools-pre-mapq-intrafrag-weighted-counts-practice\/","title":{"rendered":"Juicer Tools \u5b9e\u8dd1\u6307\u5357\u2014\u2014\u63a5\u89e6\u53bb\u91cd\u3001MAPQ\u3001\u7247\u6bb5\u8fc7\u6ee4\u4e0e\u5206\u8fa8\u7387\uff0c\u54ea\u4e9b\u6b65\u9aa4\u771f\u7684\u6539\u53d8\u4e86\u77e9\u9635"},"content":{"rendered":"
\n

\u62ff\u5230\u4e00\u4e2a\u80fd\u591f\u6253\u5f00\u7684 Hi-C \u6587\u4ef6\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u6bd4\u5bf9\u3001\u53bb\u91cd\u548c\u8d28\u91cf\u8fc7\u6ee4\u5df2\u7ecf\u5168\u90e8\u5b8c\u6210\u3002\u77e9\u9635\u521b\u5efa\u7a0b\u5e8f\u53ef\u4ee5\u5fe0\u5b9e\u5730\u6c47\u603b\u8f93\u5165\uff0c\u5374\u65e0\u6cd5\u66ff\u5206\u6790\u8005\u4fdd\u8bc1\u8f93\u5165\u4ee3\u8868\u72ec\u7acb\u5206\u5b50\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u540c\u4e00\u4efd\u63a5\u89e6\u8bb0\u5f55\u5728\u4e0d\u540c\u5206\u8fa8\u7387\u6216\u8fc7\u6ee4\u6761\u4ef6\u4e0b\u770b\u8d77\u6765\u5f88\u4e0d\u4e00\u6837\uff0c\u4e5f\u672a\u5fc5\u610f\u5473\u7740\u51fa\u73b0\u4e86\u65b0\u7684\u67d3\u8272\u4f53\u7ed3\u6784\u3002<\/p>\n

\u672c\u6587\u4e0b\u8f7d\u5b89\u88c5\u5b98\u65b9\u53d1\u5e03\u7684 Juicer Tools 2.20.00 \u6587\u4ef6\uff0c\u5b9e\u9645\u8fd0\u884c pre \u548c dump\uff0c\u7528\u5341\u4e8c\u6761\u4eba\u5de5\u63a5\u89e6\u8bb0\u5f55\u68c0\u67e5\u77e9\u9635\u6784\u5efa\u4e0e\u5bfc\u51fa\u3002\u8fd9\u91cc\u6d4b\u8bd5\u7684\u662f Juicer \u4f53\u7cfb\u4e2d\u7684\u77e9\u9635\u5de5\u5177\uff0c\u6ca1\u6709\u6267\u884c\u4ece FASTQ \u5f00\u59cb\u7684\u5b8c\u6574 Juicer \u6d41\u7a0b\uff0c\u4e5f\u6ca1\u6709\u8fdb\u884c\u771f\u5b9e\u8bfb\u6bb5\u6bd4\u5bf9\u3001\u91cd\u590d\u8bc6\u522b\u3001\u73af\u68c0\u6d4b\u6216\u6027\u80fd\u6bd4\u8f83\u3002\u6bcf\u6761\u8f93\u5165\u90fd\u7531\u811a\u672c\u6784\u9020\uff0c\u4ee5\u4fbf\u9010\u9879\u6838\u5bf9\u8f93\u51fa\u3002<\/p>\n

\"\u5efa\u597d\u63a5\u89e6\u56fe\uff0c\u8fd8\u8981\u6838\u5bf9\u8ba1\u6570\u3002Juicer
Juicer Tools 2.20.00 \u00b7 pre \u4e0e dump \u5b9e\u8dd1<\/figcaption><\/figure>\n

\u4e00\u3001\u5148\u8bf4\u6e05\u695a\u8fd9\u6b21\u8fd0\u884c\u5230\u54ea\u4e00\u5c42<\/h2>\n

\u5b98\u65b9\u5feb\u901f\u5165\u95e8<\/a>\u5c55\u793a\u4e86\u4f7f\u7528 pre \u751f\u6210\u63a5\u89e6\u6587\u4ef6\u3001\u4f7f\u7528 dump \u63d0\u53d6\u77e9\u9635\u7684\u57fa\u672c\u8def\u5f84\u3002\u5b83\u4e0e\u5904\u7406\u539f\u59cb\u6d4b\u5e8f\u8bfb\u6bb5\u7684\u6574\u5957\u6d41\u7a0b\u5b58\u5728\u4e0d\u540c\u8f93\u5165\u8fb9\u754c\u3002\u82e5\u8f93\u5165\u5df2\u7ecf\u662f\u63a5\u89e6\u8bb0\u5f55\uff0c\u5c31\u5e94\u8bf4\u660e\u4e0a\u6e38\u5982\u4f55\u4ea7\u751f\u8fd9\u4e9b\u8bb0\u5f55\uff0c\u4ee5\u53ca\u54ea\u4e9b\u8d28\u91cf\u63a7\u5236\u5df2\u7ecf\u6267\u884c\u3002<\/p>\n

\u672c\u4f8b\u4f7f\u7528\u5341\u4e00\u5b57\u6bb5\u7684\u4e2d\u7b49\u957f\u5ea6\u683c\u5f0f\uff0c\u8bb0\u5f55\u540d\u79f0\u3001\u4e24\u7aef\u65b9\u5411\u3001\u67d3\u8272\u4f53\u3001\u4f4d\u7f6e\u3001\u7247\u6bb5\u7f16\u53f7\u4e0e\u4e24\u7aef\u6bd4\u5bf9\u8d28\u91cf\u3002\u4e24\u6761\u865a\u6784\u67d3\u8272\u4f53\u5206\u522b\u957f\u4e94\u4e07\u548c\u56db\u4e07\u78b1\u57fa\uff0c\u4f7f\u7528\u81ea\u7f16\u7684\u67d3\u8272\u4f53\u957f\u5ea6\u6587\u4ef6\u3002\u8fd9\u6837\u4e0d\u4f9d\u8d56\u771f\u5b9e\u53c2\u8003\uff0c\u4e5f\u4e0d\u4f1a\u628a\u793a\u4f8b\u4f4d\u7f6e\u9519\u8bef\u5bf9\u5e94\u5230\u67d0\u4e2a\u57fa\u56e0\u3002<\/p>\n

\u5341\u4e8c\u6761\u8bb0\u5f55\u4e2d\u5305\u62ec\u4f4d\u7f6e\u76f8\u540c\u7684\u4e24\u6761\u3001\u4ea4\u6362\u4e24\u7aef\u4f4d\u7f6e\u7684\u4e00\u6761\u3001\u843d\u5728\u540c\u4e00\u5206\u7bb1\u5185\u7684\u4e00\u6761\u3001\u4e09\u6761\u542b\u4f4e\u8d28\u91cf\u7aef\u7684\u8bb0\u5f55\uff0c\u4ee5\u53ca\u67d3\u8272\u4f53\u5185\u548c\u67d3\u8272\u4f53\u95f4\u63a5\u89e6\u3002\u6211\u4eec\u8fd8\u5355\u72ec\u6784\u9020\u4e86\u7247\u6bb5\u4fe1\u606f\u5bf9\u7167\u548c\u5e26\u6743\u91cd\u7684\u8f93\u5165\uff0c\u7528\u6765\u68c0\u67e5\u5b83\u4eec\u662f\u5426\u6539\u53d8\u8ba1\u6570\u542b\u4e49\u3002<\/p>\n

\u521b\u5efa\u524d\u5148\u6309\u67d3\u8272\u4f53\u7ec4\u5408\u7ec4\u7ec7\u8f93\u5165\uff0c\u5e76\u4fdd\u5b58\u5b57\u6bb5\u5b9a\u4e49\u3002\u683c\u5f0f\u76f8\u4f3c\u4e0d\u7b49\u4e8e\u5217\u987a\u5e8f\u76f8\u540c\uff0c\u5c24\u5176\u662f\u4ece\u5176\u4ed6 pairs \u6587\u4ef6\u8f6c\u6362\u65f6\uff0c\u4e0d\u80fd\u53ea\u5220\u53bb\u8868\u5934\u5c31\u5f53\u4f5c\u53e6\u4e00\u79cd\u683c\u5f0f\u3002\u539f\u59cb\u4f4d\u7f6e\u7684\u5750\u6807\u7ea6\u5b9a\u3001\u67d3\u8272\u4f53\u547d\u540d\u548c\u4e24\u7aef\u8d28\u91cf\u6240\u5728\u5217\uff0c\u90fd\u5e94\u5728\u8f6c\u6362\u540e\u7528\u5c11\u91cf\u8bb0\u5f55\u9a8c\u8bc1\u3002<\/p>\n

\u4e8c\u3001pre \u6c47\u603b\u540c\u4e00\u50cf\u7d20\uff0c\u4e0d\u7b49\u4e8e\u8bc6\u522b\u91cd\u590d\u5206\u5b50<\/h2>\n

\u4e24\u6761\u4eba\u5de5\u8bb0\u5f55\u7684\u4e24\u7aef\u4f4d\u7f6e\u5747\u4e3a\u4e00\u5343\u4e00\u767e\u548c\u56db\u5343\u4e00\u767e\uff0c\u7b2c\u4e09\u6761\u4ea4\u6362\u4e86\u8fd9\u4e24\u4e2a\u4f4d\u7f6e\u3002\u4ee5\u4e00\u5343\u78b1\u57fa\u5206\u7bb1\u540e\uff0c\u4e09\u6761\u8bb0\u5f55\u90fd\u843d\u5728\u540c\u4e00\u4e2a\u65e0\u5411\u63a5\u89e6\u5355\u5143\uff0c\u8f93\u51fa\u8ba1\u6570\u4e3a\u4e09\u3002\u5b83\u4eec\u6ca1\u6709\u56e0\u4e3a\u4f4d\u7f6e\u76f8\u540c\u6216\u4e24\u7aef\u4ea4\u6362\u800c\u81ea\u52a8\u53d8\u6210\u4e00\u6b21\u63a5\u89e6\u3002<\/p>\n

\u8fd9\u4e2a\u7ed3\u679c\u6709\u4e24\u4e2a\u542b\u4e49\u3002\u9996\u5148\uff0c\u67d3\u8272\u4f53\u5185\u63a5\u89e6\u7684\u4e24\u7aef\u4ea4\u6362\u4e0d\u4f1a\u521b\u9020\u53e6\u4e00\u4e2a\u72ec\u7acb\u77e9\u9635\u5355\u5143\u3002\u5176\u6b21\uff0c\u5355\u5143\u8ba1\u6570\u589e\u52a0\u5e76\u4e0d\u80fd\u8bf4\u660e\u589e\u52a0\u4e86\u72ec\u7acb\u5206\u5b50\uff0c\u56e0\u4e3a\u77e9\u9635\u6784\u5efa\u8fc7\u7a0b\u53ef\u4ee5\u628a\u91cd\u590d\u8f93\u5165\u4e00\u8d77\u76f8\u52a0\u3002\u8bfb\u6bb5\u5c42\u9762\u7684\u91cd\u590d\u8bc6\u522b\u5e94\u7531\u80fd\u591f\u4f7f\u7528\u76f8\u5e94\u539f\u59cb\u4fe1\u606f\u7684\u6b65\u9aa4\u5b8c\u6210\u3002<\/p>\n

java -Xmx2g -jar juicer_tools.2.20.00.jar pre \\\r\n  -n -r 1000,5000 contacts.txt all.hic chrom.sizes\r\njava -Xmx2g -jar juicer_tools.2.20.00.jar dump \\\r\n  observed NONE all.hic chr1 chr1 BP 1000 chr1_1000.tsv\r\n<\/code><\/pre>\n
\u8fd9\u91cc\u4f7f\u7528\u4e0d\u8ba1\u7b97\u5f52\u4e00\u5316\u7684\u6784\u5efa\u9009\u9879\uff0c\u5e76\u5728\u5bfc\u51fa\u65f6\u660e\u786e\u9009\u62e9\u672a\u5f52\u4e00\u5316\u7684\u89c2\u5bdf\u503c\u3002\u8fd9\u6837\u53ef\u4ee5\u5c06\u8f93\u51fa\u9010\u9879\u5bf9\u5e94\u5230\u4eba\u5de5\u8f93\u5165\uff0c\u4e0d\u628a\u6743\u91cd\u4f30\u8ba1\u4e0e\u539f\u59cb\u6c47\u603b\u540c\u65f6\u6df7\u8fdb\u8fd9\u4e2a\u5b9e\u9a8c\u3002\u5bf9\u771f\u5b9e\u6570\u636e\uff0c\u4e5f\u5efa\u8bae\u5148\u786e\u8ba4\u539f\u59cb\u77e9\u9635\u7684\u8ba1\u6570\u5173\u7cfb\uff0c\u518d\u68c0\u67e5\u540e\u7eed\u5f52\u4e00\u5316\u7ed3\u679c\u3002<\/p>\n
\u6587\u4ef6\u540d\u5305\u542b\u201c\u53bb\u91cd\u590d\u201d\u5b57\u6837\u5e76\u4e0d\u662f\u8bc1\u636e\u3002\u4e00\u4e2a\u63a5\u89e6\u8868\u662f\u5426\u53bb\u91cd\uff0c\u9700\u8981\u67e5\u770b\u4ea7\u751f\u5b83\u7684\u6d41\u7a0b\u548c\u5b9e\u9645\u8bb0\u5f55\uff0c\u800c\u4e0d\u662f\u6839\u636e\u547d\u540d\u63a8\u65ad\u3002\u82e5\u5df2\u7ecf\u4e22\u5931\u8bfb\u6bb5\u4f4d\u7f6e\u7ec6\u8282\u6216\u5206\u5b50\u6807\u8bc6\uff0c\u4e8b\u540e\u4ec5\u51ed\u4e00\u4e2a\u8f83\u5927\u7684\u77e9\u9635\u50cf\u7d20\u4e5f\u65e0\u6cd5\u5224\u65ad\u5b83\u7531\u771f\u5b9e\u9ad8\u9891\u63a5\u89e6\u8fd8\u662f\u91cd\u590d\u5206\u5b50\u8d21\u732e\u3002<\/p>\n
\u4e09\u3001MAPQ \u95e8\u69db\u4f5c\u7528\u4e8e\u4e24\u7aef\u4e2d\u7684\u8f83\u4f4e\u503c<\/h2>\n
\u6211\u4eec\u5206\u522b\u653e\u5165\u7b2c\u4e00\u7aef\u8d28\u91cf\u4e3a\u4e94\u3001\u7b2c\u4e8c\u7aef\u8d28\u91cf\u4e3a\u4e94\uff0c\u4ee5\u53ca\u67d3\u8272\u4f53\u95f4\u63a5\u89e6\u4e2d\u4e00\u7aef\u8d28\u91cf\u4e3a\u4e94\u7684\u8bb0\u5f55\uff0c\u53e6\u4e00\u7aef\u8d28\u91cf\u5747\u4e3a\u516d\u5341\u3002\u53e6\u6709\u4e00\u6761\u8bb0\u5f55\u7684\u4e24\u7aef\u8d28\u91cf\u6070\u597d\u90fd\u662f\u4e09\u5341\uff0c\u7528\u6765\u68c0\u67e5\u95e8\u69db\u8fb9\u754c\u3002<\/p>\n
\u672a\u8bbe\u7f6e\u8d28\u91cf\u95e8\u69db\u65f6\uff0c\u5168\u90e8\u5341\u4e8c\u6761\u8bb0\u5f55\u8fdb\u5165\u672c\u4f8b\u77e9\u9635\u3002\u5c06\u95e8\u69db\u8bbe\u4e3a\u4e09\u5341\u540e\uff0c\u4e09\u6761\u542b\u4f4e\u8d28\u91cf\u7aef\u7684\u8bb0\u5f55\u88ab\u6392\u9664\uff0c\u7559\u4e0b\u4e5d\u6761\u3002\u4e24\u7aef\u90fd\u6070\u597d\u4e3a\u4e09\u5341\u7684\u8bb0\u5f55\u4fdd\u7559\uff0c\u7b26\u5408\u672c\u6b21\u7248\u672c\u7684\u5927\u4e8e\u7b49\u4e8e\u95e8\u69db\u884c\u4e3a\u3002\u4e0d\u80fd\u53ea\u770b\u4e00\u7aef\u8d28\u91cf\u5f88\u9ad8\uff0c\u5c31\u8ba4\u4e3a\u6574\u5bf9\u8bb0\u5f55\u80fd\u591f\u901a\u8fc7\u3002<\/p>\n
java -Xmx2g -jar juicer_tools.2.20.00.jar pre \\\r\n  -n -r 1000,5000 -q 30 contacts.txt q30.hic chrom.sizes\r\n<\/code><\/pre>\n
\u8fd9\u9879\u68c0\u67e5\u9002\u5408\u8fc1\u79fb\u5230\u683c\u5f0f\u8f6c\u6362\u4e2d\u3002\u5982\u679c\u8bef\u628a\u7b2c\u4e8c\u7aef\u8d28\u91cf\u5199\u5230\u4e86\u5176\u4ed6\u5217\uff0c\u8fc7\u6ee4\u6570\u91cf\u53ef\u80fd\u4ecd\u7136\u770b\u4f3c\u5408\u7406\uff0c\u5374\u5df2\u7ecf\u4e0d\u518d\u6309\u9884\u671f\u7b5b\u9009\u3002\u8bbe\u8ba1\u4e00\u6761\u53ea\u6709\u7b2c\u4e00\u7aef\u4f4e\u8d28\u91cf\u3001\u4e00\u6761\u53ea\u6709\u7b2c\u4e8c\u7aef\u4f4e\u8d28\u91cf\u7684\u5bf9\u7167\uff0c\u6bd4\u53ea\u51c6\u5907\u4e24\u7aef\u90fd\u5f88\u9ad8\u6216\u90fd\u5f88\u4f4e\u7684\u8bb0\u5f55\u66f4\u5bb9\u6613\u53d1\u73b0\u8fd9\u79cd\u9519\u8bef\u3002<\/p>\n
\u8d28\u91cf\u9608\u503c\u8fd8\u4f9d\u8d56\u4e0a\u6e38\u6bd4\u5bf9\u5668\u5982\u4f55\u5b9a\u4e49\u548c\u8f93\u51fa\u6bd4\u5bf9\u8d28\u91cf\u3002\u672c\u6587\u7684\u8d28\u91cf\u6570\u5b57\u662f\u4eba\u5de5\u5199\u5165\uff0c\u4e0d\u80fd\u636e\u6b64\u8ba4\u5b9a\u771f\u5b9e\u6570\u636e\u4e2d\u4e09\u5341\u5c31\u662f\u666e\u904d\u6700\u4f18\u95e8\u69db\u3002\u6b63\u5f0f\u6d41\u7a0b\u5e94\u7ed3\u5408\u8bfb\u6bb5\u7c7b\u578b\u3001\u6bd4\u5bf9\u7b56\u7565\u548c\u7814\u7a76\u76ee\u6807\u786e\u5b9a\u6807\u51c6\uff0c\u518d\u8bb0\u5f55\u88ab\u6392\u9664\u6570\u91cf\u53ca\u5176\u5728\u6837\u672c\u95f4\u7684\u5dee\u5f02\u3002<\/p>\n
\u56db\u3001\u53ea\u4fdd\u7559\u67d3\u8272\u4f53\u5185\u63a5\u89e6\uff0c\u4f1a\u6539\u53d8\u6bd4\u4f8b\u7684\u5206\u6bcd<\/h2>\n
\u5728\u8d28\u91cf\u95e8\u69db\u4e09\u5341\u57fa\u7840\u4e0a\uff0c\u518d\u542f\u7528\u4ec5\u4fdd\u7559\u67d3\u8272\u4f53\u5185\u63a5\u89e6\u7684\u9009\u9879\uff0c\u8ba1\u6570\u4ece\u4e5d\u53d8\u6210\u4e03\uff0c\u56e0\u4e3a\u5269\u4e0b\u4e24\u6761\u67d3\u8272\u4f53\u95f4\u63a5\u89e6\u88ab\u6392\u9664\u3002\u8fd9\u91cc\u9009\u9879\u4e2d\u7684\u201c\u5bf9\u89d2\u201d\u6307\u67d3\u8272\u4f53\u7ec4\u5408\u7684\u5bf9\u89d2\u5757\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u53ea\u4fdd\u7559\u5206\u7bb1\u77e9\u9635\u7684\u4e3b\u5bf9\u89d2\u7ebf\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u672c\u6b21\u6784\u5efa\u8bbe\u7f6e<\/th>\n\u67d3\u8272\u4f53\u5185\u8ba1\u6570<\/th>\n\u67d3\u8272\u4f53\u95f4\u8ba1\u6570<\/th>\n\u5408\u8ba1<\/th>\n<\/tr>\n<\/thead>\n
\u65e0\u989d\u5916\u8d28\u91cf\u95e8\u69db<\/td>\n9<\/td>\n3<\/td>\n12<\/td>\n<\/tr>\n
\u4e24\u7aef\u8d28\u91cf\u81f3\u5c11 30<\/td>\n7<\/td>\n2<\/td>\n9<\/td>\n<\/tr>\n
\u8d28\u91cf\u81f3\u5c11 30\uff0c\u4e14\u4ec5\u67d3\u8272\u4f53\u5185<\/td>\n7<\/td>\n0<\/td>\n7<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u6309\u8fd9\u4e9b\u8f93\u51fa\u5206\u522b\u8ba1\u7b97\uff0c\u67d3\u8272\u4f53\u5185\u63a5\u89e6\u5360\u6bd4\u4ece\u767e\u5206\u4e4b\u4e03\u5341\u4e94\u53d8\u4e3a\u7ea6\u767e\u5206\u4e4b\u4e03\u5341\u4e03\u70b9\u4e03\u516b\uff0c\u518d\u53d8\u6210\u767e\u5206\u4e4b\u767e\u3002\u6700\u540e\u7684\u767e\u5206\u4e4b\u767e\u5b8c\u5168\u6765\u81ea\u4e3b\u52a8\u6392\u9664\u67d3\u8272\u4f53\u95f4\u8bb0\u5f55\uff0c\u4e0d\u80fd\u5c06\u5b83\u89e3\u91ca\u4e3a\u6587\u5e93\u8d28\u91cf\u63d0\u5347\u3002\u6bd4\u8f83\u4e0d\u540c\u6837\u672c\u7684\u8fd9\u7c7b\u6307\u6807\u65f6\uff0c\u5fc5\u987b\u4f7f\u7528\u76f8\u540c\u8fc7\u6ee4\u9636\u6bb5\u548c\u5206\u6bcd\u3002<\/p>\n
\u7c7b\u4f3c\u95ee\u9898\u4e5f\u4f1a\u51fa\u73b0\u5728\u77ed\u8ddd\u79bb\u4e0e\u957f\u8ddd\u79bb\u63a5\u89e6\u5360\u6bd4\u4e2d\u3002\u5148\u5220\u9664\u67d0\u7c7b\u8bb0\u5f55\uff0c\u518d\u62ff\u5269\u4f59\u6570\u636e\u8ba1\u7b97\u8be5\u7c7b\u4e4b\u5916\u7684\u6bd4\u4f8b\uff0c\u4f1a\u673a\u68b0\u5730\u6539\u53d8\u6570\u5b57\u3002\u8d28\u63a7\u6307\u6807\u5e94\u5c3d\u91cf\u5bf9\u5e94\u6e05\u695a\u7684\u8f93\u5165\u9636\u6bb5\uff0c\u4fdd\u7559\u7b5b\u9009\u524d\u540e\u7684\u8ba1\u6570\uff0c\u4f7f\u8bfb\u8005\u80fd\u591f\u533a\u5206\u6570\u636e\u672c\u8eab\u7684\u6027\u8d28\u4e0e\u5206\u6790\u8005\u65bd\u52a0\u7684\u6761\u4ef6\u3002<\/p>\n
\u4e94\u3001\u7247\u6bb5\u6587\u4ef6\u4e0e\u7247\u6bb5\u8fc7\u6ee4\u4e0d\u662f\u540c\u4e00\u4e2a\u5f00\u5173<\/h2>\n
pre \u7684\u5b98\u65b9\u8bf4\u660e<\/a>\u540c\u65f6\u4ecb\u7ecd\u4e86\u9650\u5236\u6027\u7247\u6bb5\u5b57\u6bb5\u548c\u7247\u6bb5\u56fe\u9009\u9879\uff0c\u4f46\u5176\u4e2d\u5173\u4e8e\u540c\u7247\u6bb5\u8bb0\u5f55\u7684\u63d0\u793a\u4e0d\u80fd\u4ee3\u66ff\u5bf9\u5b89\u88c5\u7248\u672c\u7684\u6838\u9a8c\u3002\u6211\u4eec\u6700\u521d\u6309\u201c\u540c\u7247\u6bb5\u9ed8\u8ba4\u88ab\u6392\u9664\u201d\u8bbe\u8ba1\u9884\u671f\u7ed3\u679c\uff0c\u5b9e\u9645\u8f93\u51fa\u5374\u4fdd\u7559\u4e86\u90a3\u6761\u8bb0\u5f55\uff0c\u56e0\u6b64\u8fdb\u4e00\u6b65\u589e\u52a0\u4e86\u5bf9\u7167\u3002<\/p>\n
\u9996\u5148\uff0c\u628a\u5168\u90e8\u4eba\u5de5\u7247\u6bb5\u7f16\u53f7\u90fd\u6539\u4e3a\u96f6\uff0c\u4f46\u4e0d\u542f\u7528\u7247\u6bb5\u8fc7\u6ee4\uff0c\u8f93\u51fa\u4ecd\u7136\u662f\u5341\u4e8c\u6b21\u63a5\u89e6\u3002\u5176\u6b21\uff0c\u6784\u9020\u6bcf\u4e94\u5343\u78b1\u57fa\u4e00\u4e2a\u5207\u70b9\u7684\u865a\u62df\u7247\u6bb5\u8868\uff0c\u5e76\u6309\u4f4d\u7f6e\u91cd\u65b0\u8ba1\u7b97\u4e24\u7aef\u7247\u6bb5\u7f16\u53f7\u3002\u63d0\u4f9b\u8fd9\u4e2a\u7247\u6bb5\u6587\u4ef6\u540e\uff0c\u8f93\u51fa\u4e5f\u4ecd\u7136\u4fdd\u7559\u5341\u4e8c\u6b21\u63a5\u89e6\u3002\u8bf4\u660e\u5728\u672c\u6b21\u4e8c\u70b9\u4e8c\u96f6\u7248\u672c\u548c\u8fd9\u4e9b\u547d\u4ee4\u4e0b\uff0c\u63d0\u4f9b\u7247\u6bb5\u4fe1\u606f\u672c\u8eab\u6ca1\u6709\u5b8c\u6210\u540c\u7247\u6bb5\u6392\u9664\u3002<\/p>\n
\u968f\u540e\u663e\u5f0f\u542f\u7528\u540c\u7247\u6bb5\u6392\u9664\u9009\u9879\u3002\u5728\u6309\u4e94\u5343\u78b1\u57fa\u5207\u70b9\u8ba1\u7b97\u7f16\u53f7\u7684\u8f93\u5165\u4e2d\uff0c\u4e94\u6761\u67d3\u8272\u4f53\u5185\u540c\u7247\u6bb5\u8bb0\u5f55\u88ab\u79fb\u9664\uff0c\u5269\u4e0b\u4e03\u6761\u3002\u67d3\u8272\u4f53\u95f4\u4e24\u7aef\u5373\u4f7f\u7247\u6bb5\u7f16\u53f7\u76f8\u540c\uff0c\u4e5f\u4e0d\u4f1a\u56e0\u8fd9\u4e2a\u6761\u4ef6\u88ab\u5f53\u4f5c\u540c\u4e00\u7247\u6bb5\u3002\u8fd9\u4e9b\u7ed3\u679c\u5747\u7531\u5b9e\u9645\u5bfc\u51fa\u77e9\u9635\u9010\u9879\u6838\u5bf9\u3002<\/p>\n
java -Xmx2g -jar juicer_tools.2.20.00.jar pre \\\r\n  -n -r 1000,5000 \\\r\n  -f restriction.txt --skip-intra-frag \\\r\n  fragment_contacts.txt fragment_skip.hic chrom.sizes\r\n<\/code><\/pre>\n
\u6211\u4eec\u8fd8\u5bf9\u6700\u521d\u90a3\u4efd\u4eba\u5de5\u7f16\u53f7\u8fd0\u884c\u540c\u4e00\u6392\u9664\u9009\u9879\uff0c\u53ea\u53bb\u6389\u4e00\u4e2a\u7f16\u53f7\u76f8\u540c\u7684\u67d3\u8272\u4f53\u5185\u8bb0\u5f55\uff0c\u7559\u4e0b\u5341\u4e00\u6761\u3002\u5b83\u4e0e\u6309\u5207\u70b9\u91cd\u65b0\u8ba1\u7b97\u7f16\u53f7\u540e\u7684\u4e03\u6761\u4e0d\u540c\uff0c\u8bf4\u660e\u8fc7\u6ee4\u4f9d\u636e\u4f9d\u8d56\u8f93\u5165\u7247\u6bb5\u7f16\u53f7\u3002\u771f\u5b9e\u9879\u76ee\u82e5\u7247\u6bb5\u7f16\u53f7\u4e0d\u53ef\u4fe1\uff0c\u5373\u4f7f\u5f00\u5173\u8bbe\u7f6e\u6b63\u786e\uff0c\u4e5f\u65e0\u6cd5\u5f97\u5230\u53ef\u4fe1\u7684\u7247\u6bb5\u8fc7\u6ee4\u7ed3\u679c\u3002<\/p>\n
\u5b98\u65b9\u53c2\u6570\u89e3\u6790\u5b9e\u73b0<\/a>\u5305\u542b\u8fd9\u4e00\u72ec\u7acb\u5f00\u5173\u3002\u672c\u6587\u8bb0\u5f55\u7684\u662f\u56fa\u5b9a\u53d1\u5e03\u6587\u4ef6\u7684\u5b9e\u8dd1\u884c\u4e3a\uff0c\u4e0d\u5c06\u5b83\u65e0\u6761\u4ef6\u63a8\u5e7f\u5230\u6240\u6709\u5386\u53f2\u7248\u672c\u3002\u8fc1\u79fb\u65e7\u6d41\u7a0b\u65f6\uff0c\u5e94\u628a\u8f6f\u4ef6\u6587\u4ef6\u6821\u9a8c\u548c\u4e0e\u5c0f\u89c4\u6a21\u5bf9\u7167\u4e00\u8d77\u4fdd\u7559\uff0c\u907f\u514d\u53ea\u6cbf\u7528\u4e00\u6bb5\u53ef\u80fd\u5df2\u7ecf\u8fc7\u65f6\u7684\u8bf4\u660e\u3002<\/p>\n
\u516d\u3001\u5206\u8fa8\u7387\u53d8\u7c97\uff0c\u50cf\u7d20\u53d8\u5316\u4e0d\u7b49\u4e8e\u63a5\u89e6\u53d8\u5316<\/h2>\n
\u5728\u4e00\u5343\u78b1\u57fa\u5206\u8fa8\u7387\u4e0b\uff0c\u90a3\u4e09\u6761\u91cd\u590d\u4f4d\u7f6e\u8bb0\u5f55\u4f4d\u4e8e\u4e00\u5343\u4e0e\u56db\u5343\u5bf9\u5e94\u7684\u975e\u5bf9\u89d2\u5355\u5143\uff0c\u53e6\u5916\u4e00\u6761\u4e24\u7aef\u90fd\u5728\u4e00\u5343\u5230\u4e24\u5343\u8303\u56f4\u5185\u7684\u63a5\u89e6\u4f4d\u4e8e\u4e3b\u5bf9\u89d2\u7ebf\u3002\u6539\u6210\u4e94\u5343\u78b1\u57fa\u5206\u8fa8\u7387\u540e\uff0c\u56db\u6761\u8bb0\u5f55\u90fd\u8fdb\u5165\u96f6\u5230\u4e94\u5343\u7684\u540c\u4e00\u4e2a\u5bf9\u89d2\u5355\u5143\uff0c\u8ba1\u6570\u4e3a\u56db\u3002<\/p>\n
\u539f\u6765\u5206\u6563\u7684\u4fe1\u53f7\u53d8\u6210\u4e00\u4e2a\u66f4\u5f3a\u7684\u5bf9\u89d2\u50cf\u7d20\uff0c\u4f46\u63a5\u89e6\u8bb0\u5f55\u6ca1\u6709\u589e\u52a0\u3002\u5bf9\u4e8e\u6574\u6761\u865a\u6784\u7b2c\u4e00\u67d3\u8272\u4f53\uff0c\u4e0d\u52a0\u8d28\u91cf\u95e8\u69db\u65f6\uff0c\u4e00\u5343\u4e0e\u4e94\u5343\u5206\u8fa8\u7387\u7684\u8ba1\u6570\u603b\u548c\u90fd\u4e3a\u516b\u3002\u7b2c\u4e8c\u6761\u67d3\u8272\u4f53\u4e0e\u67d3\u8272\u4f53\u95f4\u90e8\u5206\u7684\u76f8\u5e94\u603b\u548c\u4e5f\u5206\u522b\u4fdd\u6301\u4e3a\u4e00\u548c\u4e09\u3002<\/p>\n
\u8fd9\u7c7b\u53d8\u5316\u8bf4\u660e\uff0c\u4e0d\u540c\u5206\u8fa8\u7387\u4e0b\u7684\u50cf\u7d20\u6570\u3001\u975e\u96f6\u6bd4\u4f8b\u548c\u5bf9\u89d2\u7ebf\u8ba1\u6570\u4e0d\u80fd\u76f4\u63a5\u9010\u9879\u6bd4\u8f83\u3002\u4e00\u4e2a\u7ec6\u5206\u8fa8\u7387\u4e0b\u4f4d\u4e8e\u975e\u5bf9\u89d2\u7ebf\u7684\u63a5\u89e6\uff0c\u5728\u7c97\u5206\u8fa8\u7387\u4e0b\u53ef\u80fd\u8fdb\u5165\u5bf9\u89d2\u7ebf\u3002\u82e5\u67d0\u4e2a\u540e\u7eed\u6b65\u9aa4\u4f1a\u6392\u9664\u5bf9\u89d2\u50cf\u7d20\uff0c\u6539\u53d8\u5206\u8fa8\u7387\u8fd8\u4f1a\u8fdb\u4e00\u6b65\u6539\u53d8\u53c2\u4e0e\u5206\u6790\u7684\u6570\u636e\u96c6\u5408\u3002<\/p>\n
\u77e9\u9635\u80fd\u751f\u6210\u5f88\u7ec6\u7684\u5206\u7bb1\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u6570\u636e\u652f\u6301\u540c\u6837\u7cbe\u7ec6\u7684\u7ed3\u6784\u5224\u65ad\u3002\u5206\u7bb1\u8d8a\u7ec6\uff0c\u540c\u6837\u6570\u91cf\u7684\u63a5\u89e6\u5206\u6563\u5230\u66f4\u591a\u5355\u5143\uff0c\u6bcf\u4e2a\u5355\u5143\u53ef\u7528\u8bc1\u636e\u53ef\u80fd\u66f4\u5c11\u3002\u5e94\u7ed3\u5408\u8986\u76d6\u3001\u91cd\u590d\u95f4\u4e00\u81f4\u6027\u548c\u5f85\u56de\u7b54\u7684\u95ee\u9898\u9009\u62e9\u5206\u8fa8\u7387\uff0c\u800c\u4e0d\u662f\u53ea\u6bd4\u8f83\u56fe\u50cf\u653e\u5927\u540e\u770b\u8d77\u6765\u662f\u5426\u66f4\u7ec6\u3002<\/p>\n
\u4e03\u3001NONE \u4e0d\u4fdd\u8bc1\u6570\u503c\u5c31\u662f\u539f\u59cb\u8bfb\u6bb5\u6570<\/h2>\n
\u6211\u4eec\u53e6\u5916\u6784\u9020\u4e09\u884c\u5e26\u5206\u6570\u7684\u77ed\u683c\u5f0f\u8f93\u5165\uff0c\u540c\u4e00\u63a5\u89e6\u4f4d\u7f6e\u5206\u522b\u8d4b\u503c\u4e8c\u70b9\u4e94\u548c\u4e00\u70b9\u4e8c\u4e94\uff0c\u53e6\u4e00\u4f4d\u7f6e\u8d4b\u503c\u56db\u3002\u5b9e\u9645\u6784\u5efa\u518d\u4ee5\u672a\u5f52\u4e00\u5316\u65b9\u5f0f\u5bfc\u51fa\u540e\uff0c\u7b2c\u4e00\u4e2a\u5355\u5143\u4e3a\u4e09\u70b9\u4e03\u4e94\uff0c\u6240\u6709\u5355\u5143\u603b\u548c\u4e3a\u4e03\u70b9\u4e03\u4e94\u3002<\/p>\n
\u8fd9\u8bf4\u660e\u5bfc\u51fa\u65f6\u4e0d\u5e94\u7528\u5f52\u4e00\u5316\uff0c\u53ea\u8868\u793a\u6ca1\u6709\u5728\u8bfb\u53d6\u65f6\u6267\u884c\u6240\u9009\u7684\u77e9\u9635\u5f52\u4e00\u5316\uff0c\u5e76\u4e0d\u80fd\u4fdd\u8bc1\u8f93\u5165\u6700\u521d\u5c31\u662f\u6bcf\u6761\u8bb0\u5f55\u6743\u91cd\u4e3a\u4e00\u7684\u539f\u59cb\u63a5\u89e6\u3002\u82e5\u8f93\u5165\u5df2\u7ecf\u5305\u542b\u67d0\u79cd\u5206\u6570\u3001\u7f29\u653e\u503c\u6216\u9884\u5904\u7406\u540e\u7684\u6570\u503c\uff0c\u5bfc\u51fa\u4ecd\u4f1a\u4fdd\u7559\u8fd9\u4e9b\u6765\u6e90\u7279\u5f81\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u5e26\u6743\u8f93\u5165<\/th>\n\u6c47\u603b\u5230\u7684\u5355\u5143<\/th>\n\u5b9e\u9645\u5bfc\u51fa\u503c<\/th>\n<\/tr>\n<\/thead>\n
\u540c\u4e00\u4f4d\u7f6e\u7684 2.5 \u4e0e 1.25<\/td>\n\u4e00\u5343\u4e0e\u56db\u5343\u7684\u5206\u7bb1\u5bf9<\/td>\n3.75<\/td>\n<\/tr>\n
\u53e6\u4e00\u4f4d\u7f6e\u7684 4<\/td>\n\u516b\u5343\u4e0e\u4e00\u4e07\u4e00\u5343\u7684\u5206\u7bb1\u5bf9<\/td>\n4.00<\/td>\n<\/tr>\n
\u4e09\u884c\u5408\u8ba1<\/td>\n\u4e24\u4e2a\u5355\u5143<\/td>\n7.75<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u56e0\u6b64\uff0c\u4ea4\u63a5\u63a5\u89e6\u6587\u4ef6\u65f6\u5e94\u8bf4\u660e\u6570\u503c\u5217\u7684\u6765\u6e90\uff0c\u800c\u4e0d\u662f\u53ea\u5199\u67d0\u4e2a\u5f52\u4e00\u5316\u540d\u79f0\u3002\u7279\u522b\u662f\u5728\u591a\u5de5\u5177\u683c\u5f0f\u8f6c\u6362\u4e2d\uff0c\u4e00\u5f20\u5df2\u7ecf\u53d8\u6362\u8fc7\u7684\u77e9\u9635\u5982\u679c\u88ab\u518d\u6b21\u5f53\u4f5c\u539f\u59cb\u8ba1\u6570\u5f52\u4e00\u5316\uff0c\u53ef\u80fd\u6539\u53d8\u540e\u7eed\u89e3\u91ca\u3002\u6587\u4ef6\u5bb9\u5668\u8d1f\u8d23\u4fdd\u5b58\u6570\u503c\uff0c\u6570\u503c\u4ee3\u8868\u4ec0\u4e48\u4ecd\u7136\u9700\u8981\u5206\u6790\u8bb0\u5f55\u8bf4\u660e\u3002<\/p>\n
\u516b\u3001\u8fd9\u6b21\u6ca1\u6709\u8fd0\u884c\u7684\u5206\u6790\uff0c\u4e0d\u80fd\u4ece\u77e9\u9635\u76f4\u63a5\u63a8\u65ad<\/h2>\n
\u672c\u6587\u6240\u6709\u4e3b\u8981\u6784\u5efa\u90fd\u663e\u5f0f\u5173\u95ed\u5f52\u4e00\u5316\uff0c\u5bfc\u51fa\u4f7f\u7528\u672a\u5f52\u4e00\u5316\u89c2\u5bdf\u503c\uff0c\u6ca1\u6709\u8ba1\u7b97\u6216\u9a8c\u8bc1 KR \u7b49\u6743\u91cd\uff0c\u4e5f\u6ca1\u6709\u8fdb\u884c\u663e\u8457\u63a5\u89e6\u3001\u67d3\u8272\u4f53\u73af\u6216\u7ed3\u6784\u57df\u68c0\u6d4b\u3002\u56e0\u6b64\uff0c\u793a\u4f8b\u4e2d\u51fa\u73b0\u4e00\u4e2a\u9ad8\u8ba1\u6570\u50cf\u7d20\uff0c\u53ea\u80fd\u8bf4\u660e\u4eba\u5de5\u8f93\u5165\u5728\u8be5\u5355\u5143\u805a\u96c6\uff0c\u4e0d\u80fd\u5c06\u5b83\u547d\u540d\u4e3a\u771f\u5b9e\u67d3\u8272\u4f53\u73af\u3002<\/p>\n
Juicer Tools \u7684\u4f18\u52bf\u662f\u63d0\u4f9b\u6210\u719f\u7684\u63a5\u89e6\u6587\u4ef6\u6784\u5efa\u4e0e\u63d0\u53d6\u63a5\u53e3\uff0c\u4fbf\u4e8e\u5c06\u6570\u636e\u4ea4\u7ed9\u540e\u7eed\u6d4f\u89c8\u548c\u5206\u6790\u3002\u5b83\u7684\u4f7f\u7528\u4ee3\u4ef7\uff0c\u662f\u5fc5\u987b\u660e\u786e\u8f93\u5165\u683c\u5f0f\u3001\u8fc7\u6ee4\u9636\u6bb5\u3001\u5206\u8fa8\u7387\u548c\u6570\u503c\u8bed\u4e49\u3002\u76f8\u540c\u6587\u4ef6\u6269\u5c55\u540d\u4e0d\u8db3\u4ee5\u4fdd\u8bc1\u4e24\u4efd\u77e9\u9635\u53ef\u4ee5\u76f4\u63a5\u6bd4\u8f83\uff0c\u5c24\u5176\u5f53\u5b83\u4eec\u7684\u4e0a\u6e38\u53bb\u91cd\u6216\u8d28\u91cf\u7b5b\u9009\u4e0d\u540c\u3002<\/p>\n
\u5bf9\u4e8e\u5b9e\u9645\u9879\u76ee\uff0c\u53ef\u4ee5\u5148\u6838\u5bf9\u5404\u67d3\u8272\u4f53\u7ec4\u5408\u7684\u8ba1\u6570\u603b\u548c\uff0c\u518d\u62bd\u53d6\u5c11\u91cf\u5df2\u77e5\u63a5\u89e6\u68c0\u67e5\u5206\u7bb1\u4f4d\u7f6e\uff0c\u6700\u540e\u8fdb\u5165\u5f52\u4e00\u5316\u548c\u7ed3\u6784\u5206\u6790\u3002\u6bcf\u589e\u52a0\u4e00\u4e2a\u5904\u7406\u9636\u6bb5\uff0c\u90fd\u8bf4\u660e\u5b83\u6539\u53d8\u4e86\u54ea\u4e9b\u6570\u636e\u6216\u6570\u503c\uff0c\u80fd\u8ba9\u6700\u7ec8\u53d1\u73b0\u6709\u6e05\u695a\u7684\u8bc1\u636e\u94fe\uff0c\u800c\u4e0d\u662f\u53ea\u4f9d\u8d56\u4e00\u5f20\u89c6\u89c9\u4e0a\u9c9c\u660e\u7684\u70ed\u56fe\u3002<\/p>\n
\u4e5d\u3001\u590d\u73b0\u5b9e\u8dd1\u4e0e\u8fd9\u6b21\u6392\u67e5\u7684\u7ecf\u9a8c<\/h2>\n
\u4e0b\u8f7d\u672c\u7ec4\u5b8c\u6574\u811a\u672c\u4e0e\u5b9e\u8dd1\u8bb0\u5f55<\/a>\uff0c\u5728\u672c\u7bc7\u76ee\u5f55\u6267\u884c\u4e0b\u9762\u547d\u4ee4\u5373\u53ef\u751f\u6210\u63a5\u89e6\u8868\u4e0e\u77e9\u9635\u3002\u811a\u672c\u4f7f\u7528\u5b98\u65b9\u56fa\u5b9a\u53d1\u5e03\u5730\u5740\uff0c\u5e76\u6821\u9a8c JAR \u6587\u4ef6\u6458\u8981\u3002\u5982\u679c\u8fd0\u884c\u73af\u5883\u65e0\u6cd5\u8fde\u63a5\u4e0b\u8f7d\u7ad9\u70b9\uff0c\u53ef\u5148\u4ece\u5b98\u65b9\u5730\u5740\u83b7\u53d6\u540c\u540d\u6587\u4ef6\u653e\u5230\u8f93\u51fa\u76ee\u5f55\uff0c\u518d\u8fd0\u884c\u811a\u672c\u5b8c\u6210\u6821\u9a8c\u548c\u5206\u6790\u3002<\/p>\n
python3 run.py output\r\n<\/code><\/pre>\n
\u672c\u6b21\u9047\u5230\u7684\u4e0b\u8f7d\u4e0d\u53ef\u8fbe\u95ee\u9898\u901a\u8fc7\u5728\u53ef\u8fde\u63a5\u7684\u73af\u5883\u4e0b\u8f7d\u540e\u4f20\u5165\u89e3\u51b3\uff0c\u6ca1\u6709\u66f4\u6362\u8f6f\u4ef6\u6765\u6e90\u3002\u811a\u672c\u53e6\u5916\u56fa\u5b9a\u4e86 Java \u8fd0\u884c\u5bb9\u5668\uff0c\u9650\u5236\u5bb9\u5668\u4e24\u6838\u3001\u56db GiB \u5185\u5b58\uff0c\u5e76\u4fdd\u7559\u5404\u6761\u547d\u4ee4\u4e0e\u65e5\u5fd7\u3002\u8fd9\u4e9b\u9650\u5236\u53ea\u662f\u6559\u5b66\u6267\u884c\u73af\u5883\uff0c\u6ca1\u6709\u8fdb\u884c\u5927\u89c4\u6a21\u8017\u65f6\u6bd4\u8f83\uff0c\u4e5f\u6ca1\u6709\u636e\u6b64\u63a8\u65ad\u5b8c\u6574 Hi-C \u6d41\u7a0b\u7684\u8d44\u6e90\u9700\u6c42\u3002<\/p>\n
\u6700\u503c\u5f97\u4fdd\u7559\u7684\u7ecf\u9a8c\u662f\u7528\u660e\u786e\u7684\u6b63\u53cd\u4f8b\u6838\u5bf9\u8fc7\u6ee4\u884c\u4e3a\u3002\u91cd\u590d\u4f4d\u7f6e\u3001\u5355\u7aef\u4f4e\u8d28\u91cf\u3001\u95e8\u69db\u8fb9\u754c\u548c\u540c\u7247\u6bb5\u8bb0\u5f55\u5206\u522b\u68c0\u67e5\uff0c\u53ef\u4ee5\u5b9a\u4f4d\u7a76\u7adf\u662f\u54ea\u6761\u89c4\u5219\u9020\u6210\u6570\u91cf\u53d8\u5316\u3002\u53ea\u770b\u6700\u540e\u7684\u6587\u4ef6\u5927\u5c0f\u3001\u6210\u529f\u63d0\u793a\u6216\u63a5\u89e6\u603b\u6570\uff0c\u65e0\u6cd5\u533a\u5206\u8fd9\u4e9b\u539f\u56e0\u3002<\/p>\n
\u5f53\u9700\u8981\u6279\u91cf\u6784\u5efa\u548c\u5ba1\u9605\u591a\u6837\u672c\u3001\u591a\u5206\u8fa8\u7387\u63a5\u89e6\u77e9\u9635\u65f6\uff0c\u53ef\u901a\u8fc7\u4e91\u751f\u4fe1\u751f\u7269\u5206\u6790\u670d\u52a1\u5668\u670d\u52a1<\/a>\u7edf\u4e00\u4fdd\u5b58\u5904\u7406\u73af\u5883\u4e0e\u5206\u6790\u8bb0\u5f55\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u5b9e\u8dd1 Juicer Tools \u7684\u77e9\u9635\u521b\u5efa\u4e0e\u63d0\u53d6\uff0c\u6838\u9a8c\u91cd\u590d\u63a5\u89e6\u3001MAPQ\u3001\u540c\u7247\u6bb5\u8fc7\u6ee4\u3001\u5206\u8fa8\u7387\u805a\u5408\u4e0e\u5e26\u6743\u8f93\u5165\u7684\u542b\u4e49\u3002<\/p>\n","protected":false},"author":1,"featured_media":73295,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[1064,1435,1436,82],"class_list":["post-73296","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-hi-c","tag-juicer","tag-juicer-tools","tag-82"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/juicer-tools-pre-mapq-intrafrag-weighted-counts-practice-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/73296","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=73296"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/73296\/revisions"}],"predecessor-version":[{"id":73300,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/73296\/revisions\/73300"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/73295"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=73296"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=73296"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=73296"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}